eChapters

Introduction

Electrophoresis is the migration of charged molecules in solution in response to an electric field. Their rate of migration depends on the strength of the field; on the net charge, size and shape of the molecules and also on the ionic strength, viscosity and temperature of the medium in which the molecules are moving. As an analytical tool, electrophoresis is simple, rapid and highly sensitive. It is used analytically to study the properties of a single charged species, and as a separation technique. SDS-PAGE is the most widely used method for qualitatively analyzing any protein mixtures. The method is based on the separation of proteins according to their size and then locating by binding them to a dye. It is particularly useful for monitoring protein purity and determines their relative mass. The SDS-PAGE enables the students to understand the theory behind the techniques in checking the purity of the protein and identifying the protein in a test sample.

Separation of Proteins

Proteins are amphoteric compounds; their net charge therefore is determined by the pH of the medium in which they are suspended. In a solution with a pH above its isoelectric point, a protein has a net negative charge and migrates towards the anode in an electrical field. Below its isoelectric point, the protein is positively charged and migrates towards the cathode. The net charge carried by a protein is in addition independent of its size - i.e.: the charge carried per unit mass (or length, given proteins and nucleic acids are linear macromolecules) of molecule differs from protein to protein. At a given pH therefore, and under non-denaturing conditions, the electrophoretic separation of proteins is determined by both size and charge of the molecules.

Minisatellite is a section of DNA that consists of a short series of bases 10– 60bp. These occur at more than 1000 locations in the human genome. Some minisatellites contain a central (or “core”) sequence of letters “GGGCAGGANG” (where N can be any base) or more generally a strand bias with purines (Adenosine (A) and Guanine (G)) on one strand and pyrimidines (Cytosine (C) and Thymine (T)) on the other. It has been proposed that this sequence per se encourages chromosomes to swap DNA. In alternative models, it is the presence of a neighbouring cis-acting meiotic double-strand break hotspot which is the primary cause of minisatellite repeat copy number variations. Somatic changes are suggested to result from replication difficulties (which might include replication slippage, among other phenomena). When such events occur, mistakes are made, this causes minisatellites at over 1000 locations in a persons genome to have slightly different numbers of repeats, thereby making them unique. The most highly mutable minisatellite locus described so far is CEB1 (D2S90) described by Vergnaud.

Soil microorganisms (Flora & Fauna) are just like higher plants depends entirely on soil for their nutrition, growth and activity. The major soil factors which influence the microbial population, distribution and their activity in the soil

Pulses may be defined as the dried edible seeds of cultivated legumes. They belong to the family of peas, beans and lentils (Family: Fabaceae). English word pulse is taken from the Latin word ‘Puls’, meaning pouage or thick pap. Pulses are a large family and various species are capable of surviving in very different climates and soils. Pulses are cultivated in all parts of the world and they occupy an important place in human diet.

Diet plays an important role in the management of diseases. It is very much true in the management of dogs with heart failure also. Managing the diet of a dog with heart failure is depending on the severity and stage of heart failure and presence / absence of concurrent diseases as well deficiencies.

Introduction

The ber is a vigorous-growing, small-spreading tree with almost vinelike drooping branches. The round to oval reddish brown has 5.4–8.0% sugar and 85–95 mg of ascorbic acid (Vit. C) per 100g. The tree is a host plant for rearing lac insect (Tachardia lacca). Lac insect rearing helps in the production of lac. The powder of ber roots has very many medicinal properties, such as cure for ulcer, fevers and wounds. The stem bark powder is a remedy for diarrhea. Ber also shows strong self-incompatibility (gametophytic). Madhya Pradesh, Gujarat and Chhattisgarh is a leading state in “ber” production.

The key characteristic of monsoon based agriculture of India is variability of availability of forage and fodder resources for livestock. In monsoon seasons, fodder availability will be at its peak, and the same will be scarce in dry months. Considering that, preservation of fodder during monsoon months especially through rainfed farming is essential to ensure a fodder supply throughout the year. Preservation of fodder is process of preserving the excess available fodder in a proper form without the loss of nutrients and its palatability. 

Herbicides have received wide acceptance from farmers mainly due to ease of application and effectiveness. After application, herbicide molecules should penetrate plant tissue and get translocated to the target site. Once they reach the target protein they bind to them and disturb metabolic processes in plants by disrupting biosynthetic pathways and damaging cell structures by generating cytotoxic molecules that ultimately kill the plants. In this, the first step is penetration of herbicide into the plant system.

Synergists are chemicals that significantly increase the toxicity of an insecticide while being nearly non-toxic on their own. Thus, non-toxic chemicals that contribute to the increased toxicity of insecticides are referred to as synergists. Many chemical substances have the potential to synergize with poisons. Some enzyme inhibitors work well synergistically by inhibiting enzymes that metabolize pesticides, particularly in insecticide-resistant strains. When mixed with other insecticides, some insecticides increase the toxicity of the latter at non-toxic dosages. When a mixture is more harmful than expected based on the sum of its separate efficacies when administered alone, the interaction between substances is known as synergy. Antagonistic interactions among components reduce a mixture’s efficacy.

Synergism can also develop between two or more poisons. Several statistical methods have been proposed to quantify the synergy of both non-toxic and hazardous substances. Toxin interactions are assessed using bioassays, which involve mixing toxicants (two or more) in a specific ratio. The combination is then used to prepare serial dilutions. Alternatively, the poisons can be supplied sequentially in the same amounts as in the mixture. Bioassays are also used to determine the dose-mortality response of each toxin on the same insect strain. The data are subjected to probit analysis, and the toxin interactions can be evaluated using the statistical methods listed below.

ABSTRACT

An in vitro evaluation was carried out to find out the efficiency of bacterial and fungal antagonists in checking the growth of pathogens of Kacholam. Out of the two methods tried to find out the effectiveness of Pseudomonas fluorescens against the two isolates of Ralstonia solanacearum, cross streaking produced lysis at the juncture and point inoculation produced clear inhibition zones. Among the two fungal antagonists tested Trichoderma viride was more efficient than Aspergillus niger because of its faster growth over the pathogen. However, both the antagonists showed complete overgrowth and dense sporulation on the area where the pathogen was streaked. To find out the efficacy of Pseudomonas fluorescens against leaf spot pathogens like Colletotrichum gloeosporioides and C. capsici, streaking on both sides yielded more than 50 per cent reduction in growth of both the fungal pathogens. The fungal antagonist T. viride showed a faster growth over pathogens than A. niger eventhough both recorded an antagonism index of 1500. Dense sporulation was seen over the pathogens in case of T. viride. The ability of fungal antagonists to produce volatiles and their effect on fungal pathogens were also assessed. It was noticed that the volatiles produced by T. viride was more effective than that of A. niger. Even though the extent of inhibition was less compared to direct action, this could also be one of the mechanisms of action of both the antagonists. The study revealed that all the three antagonists were effective against both bacterial and fungal pathogens and they could be utilized under field conditions.

Since the mid 1980s, genome identification and selection has progressed rapidly with the help of PCR technology. A large number of marker protocols that are rapid and require only small quantities of DNA have been developed. Three widely-used PCR-based markers are RAPDs (Williams et al., 1990), SSRs or microsatellites (Tautz, 1989), and AFLPs (Vos et al., 1995). Each marker technique has its own advantages and disadvantages. RAPD markers are very quick and easy to develop (because of the arbitrary sequence of the primers) but lack reproducibility (Karp et al., 1997; Hansen et al., 1998; Jones et al., 1999; Virk et al., 2000). AFLP has medium reproducibility but is labour intensive and has high operational and development costs (Karp et al., 1997). Microsatellites are specific and highly polymorphous (Karp et al., 1997, Jones et al., 1999), but they require knowledge of the genomic sequence to design specific primers and, thus, are limited primarily to economically important species.

The choice of a molecular marker technique depends on its reproducibility and simplicity. The best markers for genome mapping, marker assisted selection, phylogenic studies, and crop conservation have low cost and labour requirements and high reliability. Since 1994, a new molecular marker technique called inter simple sequence repeat (ISSR) has been available (Zietkiewicz et al., 1994). ISSRs are semiarbitrary markers amplified by PCR in the presence of one primer complementary to a target microsatellite. Amplification in the presence of nonanchored primers also has been called microsatellite-primed PCR, or MP-PCR, (Meyer et al., 1993).

Soil microbial biomass is an important parameter linking the plants to soil. It comprises about 2-3% of the total organic carbon in the soil and recognized as an important source of nutrients to plants. It is also a dynamic component of the soil associated with several functional properties of the terrestrial ecosystem. It represents the fraction of the soil responsible for the energy and nutrient cycling and the regulation of organic matter transformation.

Microbial biomass is a small but labile and living component of soil organic matter that is involved in most bio-geo-chemical processes in the terrestrial ecosystem. It interacts with crop productivity by regulating nutrient availability as a source; determining soil carbon storage- sink; and contributing to the atmospheric CO₂ from respiration. Thus it is the main agent that controls the flow of carbon and cycling of nutrient elements.

There is a close relationship between soil microbial biomass, decomposition rate, and nitrogen mineralization. It is also positively correlated with grain yield in organic farming and also contributes to soil structure and stabilization. Thus, there is the immense importance of microbial biomass in soil fertility as well as in agriculture.

Cereal grain contains 60 to 70% of starch and is excellent energy rich foods for humans. In almost every country and region, cereals provide the staple food. In the world as a whole, only 5% of starchy staple food comes from root crops (mainly cassava, potato, and yams, depending on climate), whereas the rest is from cereal. Cereals are an excellent source of fat-soluble vitamin E, which is an essential antioxidant. Whole cereal grains contain 20 to 30% of the daily requirements of the minerals such as selenium, calcium, zinc and copper.

The word cardiac arrest (CA) or heart attack is frightening. In animals heart attack does not occur in the manner as it occurs in humans. In humans the main cause of cardiac arrest or heart attack is coronary infarct. The prevalence of cardiac arrest or cardiopulmonary arrest (CPA) or heart attack in animals is relatively very low as coronary infarct is not a common disease in animals.

The goat rearing on extensive rearing system has been practiced since ancestry for livelihood and nutritional security of poor, landless and marginal farmers of our country with the flock size ranging from 2-10 goats. However, in the recent past, goat rearing is shifting towards semi-intensive and intensive rearing systems for commercial purposes with flock size ranging from 25 to 500. The goats reared in small flocks needs minimal housing facilities following the scientific principles with low-cost structures. On the other hand, elaborate housing is generally recommended for goats raised under large scale commercial production systems. Separate housing arrangements for different categories of animals viz. growers, pregnant females, lactating females, breeding males, etc. are to be ensured for rearing goats scientifically for harnessing maximum output sustainably.

In a cropping systems there may be simultaneous or sequential application of herbicides, insecticides, fungicides, fertilizers, PGR during a single cropping season. The different chemicals will undergo physical and chemical changes as they interact with plant, soil and other chemical substances already applied to the plant or soil. The interaction effect of such chemical substances will be visible only at the later end of the cropping season. The responses observed in the plants can be.

The actinomycetes comprise a heterologous group of prokaryotes that have the ability to form gram positive, branching filaments of less than 1μm in d.m. The main animal pathogens in the actinomycetes are in the genera Actinomyces, Arcanobacterium, Actinobaculum, Nocardia and Dermatophilus. Nonpathogenic, prolific producers of antimicrobial substances – streptomyces are also included in Actinomycetes.
Actinomyces
Natural Habitat
The actinomyces species are present on mucous membrane of the host animal, often in the oral cavity, tonsils, and nasopharynx. The soil is the natural habitat of many actinomyces species.
History
The generic name Actinomyces was first used by Harz (1879). Boestrom (1891) isolated Actinomyces bovis. Cummins (1962) clearly demonstrated Actinomyces were bacteria and they are distinct from other branching genera.

Morphology
The organisms show considerable pleomorphism. Actinomyces species are usually long and fialmentousalthough short V, Y, and T configuration also occur. In lesions of actinomycosis, the pus contains small pale yellow granules referred as sulfur granules. The sulphur granule is composed of bacterial filaments and mineralized calcium phosphate of host origin. When the granules are crushed and gram stained, a mass of gram-positive branching filaments about 1μm in width, short rods, and cocci are evident. Around this mass, a circle of club shaped bodies with their narrow ends pointing towards
the centre-staining gram negative. Hence, called ray fungus. They are nonacid fast, non-spore forming, nonmotile, non-capsulated and do not form endospores or conidia.

8.1. Meaning
The Law of Demand states that when the price of a good increases, its quantity demanded decreases, assuming all other factors remain constant. However, the law does not specify how much the quantity demanded will decrease in response to a price increase or how sensitive demand is to price changes. This information is crucial for businesses and policymakers. To address this, Alfred Marshall developed the concept of elasticity of demand, which measures the responsiveness of quantity demanded to changes in factors such as price, income, and the price of related goods.
8.2. Type of Elasticity of Demand
There are three types of elasticity of demand viz., 01) Price Elasticity of Demand 02) Income Elasticity of Demand 03) Cross elasticity of Demand
1. Price elasticity of Demand
Price elasticity measures the responsiveness of the quantity demanded of a commodity to changes in its price, while keeping all other factors constant (ceteris paribus).

ABSTRACT

Soil samples from pepper growing tracts of six districts of Kerala viz., Ernakulam, Idukki, Kollam, Kottayam, Thrissur and Pathanamthitta were assessed for total microbial population. Thirty two fungi, 17 bacteria and three actinomycetes were isolated and studied for their antagonistic potential against the foot rot pathogen, Phytophthora capsici. All the 32 fungal isolates were found antagonistic to the pathogen and majority of them belonged to the genera Trichoderma, Aspergillus, Penicillium, Fusarium, Pythium, Cunninghamella and Rhizopus. Among these maximum inhibition was recorded by Trichoderma spp. which included T. pseudokoningii, T. longibrachiatum, T. polysporum and T. harzianum. The laboratory studies were followed by field trials laid out in farmers’ fields (newly planted pepper gardens) in six districts of Kerala. Effect of biocontrol agents in wilt incidence revealed that the two-time treatment of combined application of Trichoderma local isolate and potassium phosphate was superior in checking the foot rot incidence. The treatment, Trichoderma local isolate + Bordeaux mixture applied twice came in the second position. Data on biometric observations revealed a significant increase in the number of laterals in plots treated with Trichoderma local antagonist and potassium phosphate as compared to plots treated with Bordeaux mixture and copper oxychloride. Results of quality analysis of berries revealed that oleoresin content ranged from 6.98 - 11.84 per cent and piperine content from 3.61 - 5.92 per cent and the quality remain unchanged due to various treatments. The study has provided an ‘eco and health friendly’ biological control for the management of Phytophthora foot rot, the most devastating disease of pepper.

Nutrition is the key factor affecting the production as well as the reproduction efficiency in all the animals therefore proper and balanced nutrition is the prerequisite to attain and utilize the full reproductive potential of the animals. All the nutrients like energy, protein, minerals and vitamins need to be properly balanced in the diet of animals. Energy and protein are the major nutrients required in the greatest amounts and should be in the topmost priority in order to optimize reproduction. Although minerals and vitamins are required in low amount, but very much essential as a cofactor and coenzymes in the reproductive process of the animals and deficiency of these nutrients might affect the overall reproductive efficiency of the animals.