eChapters

The average productivity of pulses (Table 1) is only 460 kg/ha but it ranges from 199 kg/ha (Moth bean) to 1719 kg/ha (Field pea). Year to year fluctuation in productivity of different pulses was also observed. The highest productivity i.e. 1067 kg/ha was observed during the year 2006-07 & the lowest productivity i.e. 497 kg/ha was recorded during the year 2008-09. The trend of area under pulse crop during the last ten years has also shown a wide fluctuation. Year to year and crop to crop variations are most pronounced.  The crop wise major pulse growing districts in Rajasthan are as follows.

Endometritis is one of the leading causes of reproductive inefficiency, infertility and decreased milk yield in high yielding dairy cows and buffaloes as well as in other species of animals and are associate with severe economic loss. Indiscriminate use of antimicrobial agents has resulted in development of resistance to the synthetic antimicrobials. Plant-derived active principles have promising potential to act as an alternative to conventional antimicrobial agents. In the present study, an attempt was made to investigate the mechanism of action of Prosopis juliflora leaves (PJ) extract against clinical isolates of E. coli.. Out of 42 clinical samples of uterine discharges and 23 isolates from cattle and buffaloes having history of uterine infection, we identified two isolates as virulent E. coli based on the cultural, biochemical and genotypic characterization. Ethanolic PJ extract showed presence of large quantity of total phenolic acid and flavonoid contents as well as promising in vitro antioxidant and radical scavenging activity. PJ extract exhibited a marked in vitro antibacterial effect against S4 isolate and reference strain (ATCC 25922) as evidenced by agar well diffusion test with MIC value of 0.39 mg/ml. This antibacterial action of PJ extract was shown to initiate at 6 h post-exposure while the complete bactericidal action was achieved at 12 h post-exposure.

Endometritis is one of the leading causes of reproductive inefficiency, infertility and decreased milk yield in high yielding dairy cows and buffaloes as well as in other species of animals and are associate with severe economic loss. Indiscriminate use of antimicrobial agents has resulted in development of resistance to the available antimicrobials. Plant-derived active principles have promising potential to act as an alternative to conventional antimicrobial agents. In the present study, an attempt was made to investigate the mechanism of action of Eucalyptus robusta leaves (ER) extract against resistant bacterial isolates. Out of 70 clinical samples of uterine discharges from cattle and buffaloes having history of uterine infection, we have identified one isolate as methicillin-resistant Staphylococcus. As per the CLSI guideline, this isolate (S4) was found to be catalase +ve, oxidase –ve and possess mecA and coaA genes along with shown resistance to cefoxitin and MIC breakpoint for oxacillin was >4 µg/ml. Methanolic extract of ER reconstituted in water showed presence of eucalyptol (marker compound) along with other major bioactive phytoconstituents following GC-MS analysis. ER extract exhibited marked in vitro antibacterial effect against S4 isolate and MRSA (ATCC 33591) as evidenced by agar well diffusion test with MIC value of 0.3125 mg/ml.

The Ramayana written by sage Valmiki is one of the great epics of the Sanskrit language. It is most religiously regarded and popular book in Hinduism. The book is believed to be complied between 300 B.C.E. – 200 AD. However, the original story could be as old 500 B.C.E. (Goldman, 1984). There are many other versions of the epic in various regional languages found in India and South Asia (Raghunathan, 1981; Arya,1998; Dutt, 2004; Mukherjee, 2015; Sharma and Arya, 2022).

As per literature references, the name ‘Ramanaya’ is composed of two words, ‘Rama’ and ‘Aya?a’. Rama, the name of the main character of the Epic, has two contextual meanings. In the Atharvaveda, it means 'dark’, dark-coloured, ‘black' and is related to the word ‘ratri’ (night) which means 'darkness of night'. The other meaning, which can be found in the Mahabharata, is 'pleasing’, pleasant, charming, lovely, beautiful. The word ‘Ayana’ means travel or journey. Thus, Ramaya?a means ‘Rama's Journey’ from his kingdom to various places, forests during his life time (Anonymous, 1960; Tulsidas, 1966; Murthy, 2003; Kishore, 2007; Rajagopalachari, 2013).

Ramayana contains over 24,000 couplet verses, in its full form, which are divided into following seven ‘Ka??as’ (booklets) - Balaka??a, Ayodhyaka??a, Ara?yaka??a, Ki?kindhaka??a, Sundaraka??a, Yuddhaka??a, Uttaraka??a and about 500 sargas (chapters). It is regarded as one of the longest epic poems written in history (Keshavadas, 1988; Debroy, 2017; Gupta, 2021).

morally right man in the world? Narada replied that such a man is Rama, son of King Dasharatha of Ayodhya. Valmiki wanted to know more about Rama and proceeded to take bath when he saw two birds in playful mood and was happy. Suddenly, a hunter hiding in the bush shot one of the two birds. Valmiki became painful and chanted some dialogues in curse which ultimately created a new form of sloka. This was the beginning of the main narrative (Goldman, 1984; Sachithanantham, 2004).

The rambutan is native to Malaysia and commonly cultivated throughout the archipelago and southeast Asia. Though a close relative of the lychee and an equally desirable fruit, this member of the Sapindaceae is not nearly as well-known. Botanically, it is Nephelium lappaceum L. (syns. Euphoria nephelium DC.; Dimocarpus crinita Lour.). In the vernacular, it is generally called rambutan (in French, ramboutan or ramboutanier; in Dutch, ramboetan); occasionally in India, ramboostan.

Genus : Nephelium 
Species : lappaceum L.
Family : Sapindaceae
Chromosome No. : 2n = 22

Rambutan is one fruit from the Malay Archipelago which has become very popular in its place of origin and is well distributed here. Besides, rambutan a number of other valuable tropical fruits are present which are yet to be cultivated extensively in other tropical and subtropical parts of the world. In Singapore and Penang the fruit grows in abundance. Though very popular in Malay Archipelago, Sri Lanka, however it is unknown in India, Madagascar, Mauritius etc. The fruits common name has been derived from Malayan word rambut referring to hairs by which the fruit surface is covered with. The fruit is also known by other names like Rambustan, rambotang, ramboetan etc. The crop is strictly tropical in climate requirements and is very sensitive to frost. For best performance it likes a hot and moist climate and is a major factor why the crop has not succeeded in most other places.

A. Diseases

1. Alternaria leaf spot

Occurrence & severity status

In the years 2012 and 2013, black leaf spot disease was observed on ramie plants in Hunan and Hubei Provinces, China (Yu et al., 2016). Yu et al. (2016) claimed that their report was the first report of Alternaria leaf spot of ramie in China.

Symptoms

In the field, the symptoms of this disease included dark green to black big spots on leaves (Fig. 20A), often resulting in upwardly curled leaf margins (Yu et al., 2016).

Ramsar sites, designated under the Ramsar Convention on Wetlands, play a crucial role in global biodiversity conservation by providing vital habitats for numerous species and maintaining ecological processes. These sites are often located within biodiversity hotspots, regions characterized by high levels of endemic species and significant habitat loss. The intersection of Ramsar sites and biodiversity hotspots highlights the importance of conserving wetlands, which serve as critical ecosystems for both terrestrial and aquatic life. This paper explores the ecological significance of Ramsar sites, their contributions to sustaining biodiversity, and the challenges they face due to climate change, pollution, and urbanization. Through effective management and conservation strategies, Ramsar sites can enhance global efforts to preserve biodiversity and mitigate the impacts of environmental degradation, ensuring a sustainable future for both wildlife and human communities.

RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR reaction, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (8-12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from a RAPD reaction.

No knowledge of the DNA sequence for the targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. This makes the method popular for comparing the DNA of biological systems that have not had the attention of the scientific community, or in a system in which relatively few DNA sequences are compared (it is not suitable for forming a DNA databank). Because it relies on a large, intact DNA template sequence, it has some limitations in the use of degraded DNA samples. Its resolving power is much lower than targeted, species specific DNA comparison methods, such as short tandem repeats. In recent years, RAPD is used to characterize, and trace, the phylogeny of diverse plant and animal species.

RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR reaction, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (8-12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from a RAPD reaction.

No knowledge of the DNA sequence for the targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. This makes the method popular for comparing the DNA of biological systems that have not had the attention of the scientific community, or in a system in which relatively few DNA sequences are compared (it is not suitable for forming a DNA databank). Because it relies on a large, intact DNA template sequence, it has some limitations in the use of degraded DNA samples. Its resolving power is much lower than targeted, species specific DNA comparison methods, such as short tandem repeats. In recent years, RAPD is used to characterize, and trace, the phylogeny of diverse plant and animal species.

Section-A : Write True / False

Q.1 A random variable (r.v) is a real number x connected with the outcomes of a random experiment.

Q.2 In tossing of a balanced die at one time, the number coming on the upper most face of the die is a random variable x which takes any one out of 6 values taken as 1, 2, 3, 4, 5, 6.

Q.3 Let s be the sample space associated with a given random experiment. A real valued function defned on s and taking values in R (-∞,∞) is called one dimensional random variable.

Q.4 In general a n dimensional random variable is simply a function whose domain is S and whose range is a collection of n-tuples of real members (vectors in n-space).

Introduction

In any experiment, for instance, a varietal trial or a treatment trial, the main purpose of the experimental design would be to detect the differences among the varieties or treatments. This assumes, as an obvious prerequisite that the experimental units, such as plots or animals, be as alike as possible so that differences observed in responses, such as yield in varietal trial, could be attributable to the varietal or treatment differences. But more often than not, this condition is not realized to a satisfactory degree in practice due to heterogeneity present in experimental units. In a field experiment, for instance, a big piece of land might be quite heterogeneous regarding soil fertility, moisture content, salinity, etc; so that experimental plots marked on this land would differ widely among themselves, though plots nearer to each other would be more alike or homogeneous than the plots scattered distant apart; also in the case of animal experiments, animals forming the experimental units might be heterogeneous regarding their ages , weights, genetic composition, etc. With such experimental units in the experiment, the variability produced in the observations will be comparatively high so that only large treatment differences are detectable, since small differences are obsecured by large error variability.

The randomized complete block design (RCBD), is perhaps the most widely used design of all experimental designs, mainly for its simplicity, flexibility and validity. The main objective of the design is either to control, remove or reduce the variability due to heterogeneity in experimental material, which is quite irrelevant for treatment comparisons. In CRD, the error variability as estimated from the residual sum of squares will be considerably high. The RCBD is a device introduced in this design for separating out the variability due to heterogeneity among experimental units from the variability due to all extraneous sources, thus purifying in one sense. The design is especially suitable for field experiments where the number of treatments is not large and the experimental area has a predictable productivity gradient.The primary distinguishing feature of the RCBD is the presence of blocks of equal size, each of which contains all the treatments.

This chapter provides a comprehensive overview of the randomized block design (RBD), detailing its theoretical concept, layout, and a detailed segment on pooled ANOVA concepts. It explains the mathematical models for RBD and how to interpret the results from ANOVA tables in this context. The chapter also offers step-by-step guidance for analyzing RBD, Factorial RBD, and pooled RBD ANOVA using R.

Randomized Block Design

The RBD is a widely used experimental design in statistics and research, particularly when dealing with certain specific conditions:

• Homogeneity within blocks: RBD is ideal when there is inherent variability in the experimental units, but this variability can be grouped into homogenous blocks. Each block contains a complete set of treatments, and the blocks themselves account for the variability.

• Principles of experimental design: RBD adheres to the core principles of experimental design, namely randomization, replication, and local control. Randomization is used within blocks to assign treatments, ensuring that the comparison of treatments is fair.

• Replication across blocks: In RBD, each treatment is replicated across different blocks. This replication enhances the reliability and robustness of the experimental results.

• Managing uncontrolled variability: RBD is particularly effective in field experiments or situations where external variability (e.g., soil type, light conditions) cannot be completely controlled. By grouping similar units into blocks, RBD effectively reduces the impact of this uncontrolled variability on the treatment effects.

• Improvement in experimental accuracy: The use of blocks to organize the experimental units usually leads to a reduction in the error variance compared to CRD. This enhances the accuracy and precision of the experiment.

Objective: To amplify DNA using RAPD primers and study on agarose gel.

Background information:

RAPD markers are decamer (10 nucleotide length) DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals. RAPD is applied to analyze the genetic diversity by random primers. Unlike traditional PCR analysis, RAPD does not require any prior DNA sequence information of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions complementary with primers' sequence. For example, no fragment is produced if primers annealed too far apart or 3' ends of the primers are not facing each other. Therefore, if a mutation has occurred in the templ that was earlier complementary with primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. RAPD is a dominant marker means it cannot distinguish between heterozygous; single copy and homozygous; two copies. Rarely RAPD markers may be Co-dominant where different fragment length of DNA is amplified from the single locus. PCR is a molecular technique used for amplification of DNA, a complete enzyme based chemical reaction, therefore, the concentration and quality of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible. Mismatches between the primer and the template may result in the total absence of PCR product or decreased amplified product. Thus, the RAPD results can be difficult to interpret.

1. ‘Agrostoogical’ refers to cultivation of_____________. (Grasses)
2. Land on which the potential plant community is composed periodically of native grass, forbs and shrubs valuable for forage and in sufficient quantity to justifying grazing use is called as_____________. (Rangeland)
3. The basic object of range management is to_____________ and_____________ the rangeland for higher production of fodder, rich in nutritive value and palatability. (Improve & maintain)
4. ICAR had carried a grassland survey of India in 1954 by Wnyte, Venkatramanan and Dabadghao had found_____________ types of grass land in different environment. (8)
5. The main reason for the enormous increase in livestock population is_____________. (Social attitude of the people)
6. All the vegetation consumed by grazing animals including fruits and twigs of trees and shrubs is called_____________. (Pasturage & forage)
7. The practice by which the gap can be bridged between the requirement and availability of animal feeds and fodder is known as_____________. (Grass land techniques)
8. The scientists_____________ have recognised five type of grass covers in India. (Dabadghao & Shankar Narayan, 1979)
9. The maximum area of about 2.8 milloin square kilometre is covered under_____________ type of grassland in India. (Phragmites Saccharum type)

14.1 Introduction

The theory of simple correlation (r_xy), regression coefficients (b_yx & b_xy) and their test of significance have been discucsed in chapter 13. The test of significance of observed partial correlation coefficient p₁₂₃(k+2) and multiple correlation coefficient R₁₂₃, have also been discussed in sections 13.4 and 13.5 respectively. Now in this chapter mainly the process of how to calculate spearman’s rank correlation coefficient, partial correlation and regression coefficients and multiple correlation coefficient using their fundamental definitions as well as appropriate statistical formule in terms of pairwise simple correlation coefficient r_ij (i = j = 1, 2, 3) in trivariate populations have been provided to make the students and its users to be familiar with basic and crystal clear concepts of the subject matter with the help of an empirical studies based on some original data on (X₁, X₂, X₃). Here X₁ has been considered as study variate and (X₂, X₃) as covariates.

This family is represented by about 62 genera and 2,252 species, chiefly distributed in the temperate regions of the Northern Hemisphere. Eastern Asia and eastern North America are the regions of their most common occurrence. The common genera, of which over 150 or more species have so far been reported, include Ranunculus (400), Aconitum (300), Delphinium (250), Clematis (250), Anemone (150) and Thalictrum (150). Some species occur in Arctic and Alpine zones. However, they are rare in tropics. Twenty genera and over 165 species of this family have so far been reported from India. Plants of this family flower mainly during winter in our country. Selected Indian Genera and Species
• Ornamentals: Many Ranunculaceae have showy flowers and are grown as ornamentals in gardens. Some of such ornamentals are Aconitum napellus (aconite), Anemone parviflora (wind flower), Aquilegia coerulea (columbine), Caltha palustris (Marsh marigold), Clematis virginiana (traveller’s joy), Delphinium ajacis (larkspur), Helleborus (Christmas rose), Nigella damascena (love-in-a-mist), Paeonia emodi (Peony), Ranunculus aquatilis, R. seleratus (cedery peaved crow foot).

ABSTRACT

Random Amplified Polymorphic DNA (RAPD) markers were used for characterizing eleven banana (Musa AAB Plantain Subgroup) clones using 40 decamer primers. Eight primers, which produced reproducible banding patterns, yielded 42 scorable bands with an average of 5.25 bands per primer. The amplification products ranged in size from 400 to 1500 bp. A genetic similarity matrix was constructed using the Jaccard’s coeffecient method. The pair wise similarity coefficient values varied between 0.3333 and 0.9355. Based on the coefficients, distances between the clones were computed using SYSTAT software package. In the dendrogram constructed by the nearest neighbour (single- link) method (Krzanowski, 1988), all the eleven clones were found grouped under five clusters. Attu Nendran, Changanasseri Nendran, Changazhikodan, Kaliethan, Koonoor Ethan and Quintal Banana formed the largest cluster. Manjeri Nendran and Myndoli formed the second cluster. Padalamurian, Mysore Ethan and Zanzibar formed three separate clusters. Zanzibar, belonging to the Horn Plantain group was different from the rest of the clones.

ABSTRACT

There is a wide variability in the contents of the commercially important chemical compounds of Centella asiatica depending on the source / ecotypes of the plants. The glycosides found in Indian plants include brahmoside, brahminoside and minor amounts of asiaticosides, which vary in their relative contents depending on the ecotypes. An RAPD marker assisted study of ten accessions, collected from different geographical locations of Kerala, viz., Parumala (Kottayam District), Kottayam, Nagercoil (Tamil Nadu), Mananthala (Trivandrum District), Vlathankara (Trivandrum District), Ponmudi (High range, Trivandrum District), Angamaly (Ernakulam District), Punalur (Kollam district), Pattambi (Palakkad district) and Calicut, was conducted. DNA amplification of the accessions was studied using forty decamer primers, involving 43 cycles. Based on the number of bands four primers were selected. The least similarity coefficient of 0.71 was shown by the Pattambi accession, which formed a separate cluster with all other accessions. Nagercoil and Ponmudi accessions showed the highest similarity coefficient of 0.88 with each other.