eChapters

Soybean [Glycine max (L.) Merrill] is the leading oilseed crop in the world. Its area and production are far ahead of other oilseeds at the global level (Table 1). The commercial cultivation of this crop in India started in the early 1970’s. Within a span of 4 decades its spread has been phenomenal and today it occupies around 9.67 million hectares with a production of about 10.22 million with an average productivity of around 1006 kg ha^-1 (DSR, 2010) (Table 2). It is now the fourth largest among field crops after rice, wheat and maize (GOI, 2007) in India and has become the leading oil seed crop surpassing groundnut and mustard and rapeseed. It is contributing nearly 37% to the total oilseeds produced in India. The central India comprising the states of Madhya Pradesh, Maharashtra and Rajasthan account for more than 95% of the total area under this crop. Chhattisgarh, Karnataka, Andhra Pradesh, Uttar Pradesh etc account or the bulk ofthe rest ofthe area. It is predominantly a rainfed crop with only about 3% of the total area getting some assured irrigation facility. Further, it is concentrated in areas which receive lesser rainfall in its early vegetative phase. A large number of crops have been replaced by soybean to a variable extent in its march towards achieving its present position. Badal et al. (2000) have reported that a large number farmers switched over to this crop from sorghum, groundnut, maize, cotton etc in the state of Madhya Pradesh owing to greater yield potential even under adverse conditions (Table 3). Further, it has become an integral part of several cropping systems including both intercropping and-sequential cropping systems in different parts of the country.

The apparent reasons for undesirable productivity under intensively cultivated areas are depletion of native nutrient reserves, emergence of multinutrient deficiencies, and consequent decline in factor productivity of applied nutrient (Dwivedi et al., 2006; Yadav, 1998; Yadav, 2003). Surveys conducted at PDFSR in different agro-climatic regions of Indo- Gangetic Plain indicated that in order to achieve previously attained yield levels, farmers have started applying greater doses of N than the recommended ones (Dwivedi et al., 2001; Sharma, 2003). Such indiscriminate use of N is likely to further worsen the nutrient imbalance in soil-plant systems, besides increasing the pest incidence, cost of production and environmental problems (Dwivedi et al., 2001). The annual productivity of rice - wheat could hardly exceed 9 t ha^-1 in the multilocation experiments on yield maximization (Dwivedi and Singh, 2011). On the other hand, long-term experiments (Singh and Mishra, 2010) and other studies indicate that crop productivity can be sustained with balanced fertilization, taking into account the emerging deficiencies of secondary and micronutrients (Tiwari et al., 2006)

Nutrient management is a major component of a soil and crop management system. It becomes even more important in intensive high yielding cropping systems because of the higher investments at stake. The SSNM is applying those concepts to areas within a field that are known to require different management options from the field average. Site specific nutrient management is a concept that can be applied to any field and any crop. Yet, it is most often thought of in relation to use of computer and satellite equipment, and does not require large farming operation. The technology tools certainly expand the capabilities for using site-specific management. This is really a “repackaging“ of management concepts that have been developed and promoted for many years.

India is a leading vegetable producing country in the world. Presently it occupies 7.981 million hectare area with the annual production 129.077million tones (2008-09). The country being belessed with the unique gift of nature of diverse climate and distinct seasons, make it possible to grow an array of vegetables number exceeding more than hundred types. However, potato being the staple food and easy to mix in several preparations ranks first (26.6%) in total production of vetetables followed by onion (10.5%) and other important Solanaceous vegetables like tomato (8.6%), brinjal (8.0%). Cauliflower and cabbage are most preferred winter vegetables and their total share in the country’s vegetable production is 5.1 and 5.3%, respectively. Other important vegetables, which are primarily grown in the country, are okra, onion, vegetable peas and a good range of cucurbits (Fig. 1)

There is miss-match in area production and productivity of vegetables among various states of the country. The West Bengal ranks first in the total production of vegetables in the country and its production during the year 2008-09 was in tune of more than 22.704 million tones, contributing the more than one-fifth of total country’s vegetable product ion. Uttar Pradesh, Bihar and Orissa in north India and Maharashtra, Tamil Nadu and Karanataka in south India are the other leading vegetable producing states in the country. In last one decade, there has been considerable progress in enchancing the productivity of vegetables which is presently 16.2 tonnes hectare^-1. Incessant growth of urbanization, ceaseless fragmentation ofland holdings and depleting natural resources are the major challenges before the expansion of any agricultural commodity either cereals or vegetables. Hence, our attention must be focused on vertical expansion strengthened with the boon ofthe technology instead of horizontal expansion just by increasing the crop area. To ensure the nutrition security of the burgeoning population of the country, it is estimated that up to 2020, the country’s vegetable demand would be around 155 million tones (Rai et al., 2004; Pandey et al., 2009). To achieve this target, it is sine qua non to integrate the various technologies right from production to post-harvest.

Protected cultivation of horticultural crops offers the best choice for diversification from traditional agriculture production system for a number of reasons. Production of crops under protected structures has great potential in augmenting production and quality of vegetables, flowers and in some fruit crops in main and off-season and maximizing water and nutrient use efficiency under varied agro-climatic conditions of the country. This becomes relevant to growers in India who have small land holding. They would be interested in a technology, which helps them to produce more crops each year from their land, particularly during off-season when prices are higher. The commonly protected structures are polyhouses, poly-tunnels, floating row covers, clotches, shade-net, insect-proof net and plastic-mulches (Chandra et al., 2000 ; Singh et al. 2010). These protected structures act as physical barrier and play a key role in integrated pest management by preventing spread of insect-pests and viruses which causes severe damage to the crop (Singh et al., 2003). This technology has great potential especially in periurban agriculture in near future, since it can be profitably used for growing high value vegetable crops like tomato, cheery-tomato, coloured peppers, parthenocarpic cucumbers, cut-flowers like rose, carnation, Berbera, lilium and fruits like strawberry, grapes etc. and for off-season cultivation of vegetables and their healthy and virus-free seedling production.

Protected cultivation is intended to mean some level of control over plant micro-climate to alleviate one or more of abiotic stresses for optimum plant growth. The microclimatic parameters being referred here are temperature, light, air-composition and the nature of rooting medium.

Indian horticulture has shown impressive impact on production, productivity and profitability. Currently, the total production of horticultural produce is 214.7 million tones from the 9% of total cultivated area in India, which contribute 30.4% to GDP of agriculture (Singh, 2010). In world perspective, India is second largest producer of fruits (68.5 mt) from 6.10 m ha area and contributes 11.2% in global fruit production. Vegetable crops occupying 8.0 m ha have the production of 129.1 mt (Table 1). However; India is the largest producer, consumer and exporter of spices and spice products in the world and produce more than 50 spices. The spice production in India is the order of 4.14 mt from an area of about 2.63 m ha. India is also the leading producer of plantation crops in the world and contributes 22.34% in coconut, 25% in cashewnut and 55% in arcanut. Commercial floriculture sector has recorded fast pace of growth during the last decade. During the last decade area under floriculture has expended to 1,67,000 ha with production of 9,87,000 MT of loose flower and 4.8 million cut flowers (Singh, 2010). The over all percent share of fruits and vegetables to their total production and contribution of different states for production of fruits and vegetables are repicted in Fig. 1 & 2. From the above fact, it is indicated that the production of horticulture produce has increased manifold but the gap in demand and supply continues simultaneously and is hardly sufficient and meets only 46% of national demand. Hence, there is a strong need to increase the production and productivity through crop diversification.

Agriculture in India is one of the most important sectors of its economy. It provides livelihood to almost two thirds of the work force in the country and accounts for 18% of India’s GDP. About 43 % of India’s geographical area is used for agricultural activity and large number of production systems are in practice in different parts ofthe country. In pre-independence era (before the 1950s), agriculture in India was a system of harnessing nature for the sustenance of human beings, similar to the presently defined organic farming. Some countries have moved away from inorganic to organic farming yet it is not possible for India to depend entirely upon this system of farming due to large population. Total availability of plant nutrients from organic sources is projected to be 7.75 million tonnes by 2025 (Tandon, 1997) against the total requirement of37.50 million tonnes (Katyal, 2001) for 1504 million population (Sekhon, 1997). Thus nutrients supply through organic sources alone would result in deficiency of about 30 million tonnes of nutrients. Hence, it is imperative to use both organic and inorganic sources of plant nutrients enhance food production for the increasing population (Mahajan et al., 2007). In India the contribution of organic sources was 80-100 per cent in 1949-50 and reduced to about 32 per cent in 1993-94 and 20-25 per cent in 2004-05. So, it is imperative to enhance organic sources to meet total nutrient consumption. Following independence, rapid population growth in India placed great pressure on land and on these traditional farming systems; huge demands for food grains led to increased use of fertilizers and pesticides to boost production. Still a negative balance of about 8 million tones of NPK is foreseen in 2020, even if we continue to use chemical fertilizers, maintaining present growth rates of production and consumption. The most optimistic estimates at present, show that only about 25-30 per cent nutrient needs of Indian agriculture can be met by utilizing various organic sources. Many of the gains in production during the last 4-5 decades resulted from the “Green Revolution,” a campaign of technological interventions in agriculture widely adopted by farmers in developing countries. Expansion of irrigation to cover rain fed areas, popularization of hybrids/ transgenic varieties of crops, and use ofsynthetic chemical fertilizers and pesticides were the maj or technologies promoted. This paid rich dividends in India, quadrupling food grain production from 50 million metric tons in 1950-51 to 211 million metric tons in 2001-02, and enabling India to become self-sufficient in food grain. Now a second green revolution is also in the offing, to boost agricultural production and meet the estimated requirement of 337 million metric tons.

Rice-wheat cropping system is the most important food production system in India. This system has enabled the country to attain food security during last three decades and provided livelihood to millions of farming families in recent times. The productivity of this system has now started showing signs of decline or stagnation at many places. There are also concerns of declining soil health and other related environmental problems. At the same time, food demand is rapidly increasing due to increasing population and income growth. During the past 30 years, agricultural production has been able to keep pace with population demand for food. This came about through significant area and yield growth. Area growth was a result of new lands being farmed and through increases in cropping intensity, from a single crop to double or even triple crops per calendar year. Area growth will be less important in its contribution to production growth in the future as more land is used for urban areas and industry. Yield growth will have to be the mainstay for providing the means for meeting future food demands unless food imports start to play a major role. Total factor productivity is declining and farmers have to apply more fertilizer to obtain the same yields. There is, therefore, a huge challenge ahead to meet future food demands without damaging the natural resource base on which agriculture depends, producing food at a cost that is affordable by the poor, and with incentives to farmers that allow them to improve their livelihoods. Water will become a major limiting factor for sustained production in the next decade. Rapidly growing urban areas and industry will compete with agriculture for good quality water. There are already reports of declining water tables in some areas leading to more costly pumping of groundwater and increased costs of production.

This chapter describes various conservation agriculture technologies to attain the goal of increasing productivity and meeting food security needs while at the same time efficiently using natural resources, including water, providing environmental benefits and improving the rural livelihoods of farmers. The major success in the last few years has been the development and deployment of conservation agriculture technologies (CATs) with farmers in the rice-wheat systems. CATs range from simple surface seeding, where wheat seed is broadcast on the non-ploughed soil; no-till with a special opener for placing seed in the soil also without ploughing; reduced tillage and bed planting. This has benefited farmers through less cost, more yields and more income. CATs are defined as any practice that will result in improvement of the efficiency of natural resources. These technologies are rapidly gaining popularity among farmers as they result in higher production at less cost with significant benefits to the environment and more efficient use of natural resources. This ultimately results in higher profits, cheaper food, and improved farmer livelihoods. Crop diversification is also easier as less land is needed to produce staple cereals, freeing up land for other crops.

Biodiversity is the variety of life in different microbes, animals, plants and their genes present in the ecosystem. Soil provides a vital habitat and environment primarily for microflora such as bactria, actinomycetes, fungi as well as microfauna viz. protozoa and nematodes, mesofauna such as microarthopods, enchytraeids, macrofauna such as earthworms, beetles, termites and millipedes (Gaur 2006a).

Biodiversity and soil are interlinked which interact closely with wider biosphere. Conversely, biological activity is ofprime importance in influencing the physical and chemical conditions of soil (Bardgett 2005). The level of soil biological activity is, therefore, affected by soil type and also depends on the use of management practices which contribute to crop productivity.

Soil biodiversity and soil processes

A majority of soil organisms participate in various processes which are essential for the biosphere. The main ecological function is to decompose organic matter largely derived from the plant and animals residues Which consist of celluloses, hemicelluloses and lignin, and to supply plant nutrients to support growth. These organisms participate in various nutrient cycles and processes such as nitrogen mineralization, nitrification, nitrogen fixation, transformations ofphosphorus, potassium, sulphur, calcium, magnesium, iron and micronutrients (Pankhurst et al 1997). They participate in bioremediation of harmful compounds such as heavy metals and pesticide residues (Gaur 2006 b). They not only maintain physical and chemical conditions of soil, but also regulate emissions / assimilations of green house gases and suppress soil borne diseases and pests

A nutrient budget is a valuable indicator of the long term sustainability of a cropping system. It indicates where fertilizer application is inadequate and leading to decline in the soil nutrient status. Conversely, it can indicate excessive inputs which result in a nutrient surplus and greater potential for losses to the environment and waterways. (Combs et al., 1996) Thus nutrient budget is the comparison between all the sources of nutrients available to the producer and the requirement of nutrients to meet the crop and soil needs. The sources can either be from on the farm, such as livestock manure or credits from legumes, or from off the farm, such as purchased fertilizer or irrigation water. The requirement is the amount of nutrients needed by the crop to obtain the expected yields.

Most values for nutrients available from different sources (credits) and crop nutrient requirements are calculated from estimates taken from many years of long historical averages and field research. There is no “real time” method of calculating exactly what neither the crop’s nutrient requirement or the nutrients available are at any one time. More closely, both the nutrient requirements and availability are based on past performance for the climatic and soil condition. These values are given with some surety that the crop grown will be supplied with adequate nutrient during the growing season and the crops will not be limited in its growth. All incidental environment losses, such as runoff and leaching, have been accounted for. Climatic conditions, particularly temperature and soil moisture, greatly influence both the crop performance and the soil’ s capacity to provide nutrients to the plant. During any growing season, there can be many changes in the climate conditions that will affect both the crop growth and soil delivery of nutrients to the crop.

Although a nutrient budget is not an exact formula for supplying nutrients, it is one method for organizing the nutrient needs ofthe crop with the nutrients available on the farm. Nutrient budgets can easily determine if there is a gross imbalance between the nutrients that are available vs. the amount required. Nutrient budgets are one of the best methods to see the over all supply of crop nutrients available, compared to the estimated crop needs as given by historic records and field research. Continued use of soil testing, plant and water analyses and yield monitoring are essential to maintain good nutrient balance with desired results.

Organic C in agricultural soils contributes positively to soil fertility, soil tilth, crop production, and overall soil sustainability. Changes in agricultural management can potentially increase the accumulation rate of soil organic Cd (SOC), thereby sequesteringCO₂fromtheatmosphere.Optimizingagricultural management for accumulation of SOC can result in the sequestration of atmospheric CO₂, thereby partially mitigating the current increase in atmospheric Carbon dioxide. In addition to the environmental benefits of soil C sequestration, Carbon dioxide consideration has also been given to the implementation of a C credit trading system which may provide economic incentives for C sequestration initiatives.

Changes in agricultural practices for the purpose of increasing SOC must either increase organic matter inputs to the soil, decrease’decomposition of soil organic matter (SOM) and oxidation of SOC, or a combination thereof. These practices include, but are not limited to, reducing tillage intensity, decreasing or ceasing the fallow period, using a winter cover crop, changing from monoculture to rotation cropping, or altering soil inputs to increase primary production (e.g., fertilizers, pesticides, and irrigation). Implementing practices that sequester C can reverse the loss of SOC that may have occurred under intensive cultivation thereby increasing SOC to a new equilibrium

Balanced crop nutrition is a most reaJized critical factor in allowing crops to give their full yield potential. The application of macro and micronutrients through manures and fertilizers to achieve this balance is an integral practice in modern crop production system. Macro and microelements have long been recognized as being associated with changes in the level of many diseases and yield and /or quality of crops. Three major elements N, P, and K are the most studied nutrients which chiefly affect relative resistance of crop plants to various diseases. Others macro/micro/beneficial nutrients including Ca, Mg, S, Mn,Te, Zn, Mo, Si, Bo and Cu have shown varying degrees of disease suppression/induction on many crop species under various environmental conditions. Crop nutrition influence plant health through: i. affecting the growth, reproduction, virulence, and survival of pathogens; ii. resistance and tolerance, predisposition and escape in the host crop; and iii. root exudates and microbial interactions facilitatingbiological control. Balanced crop nutrition can protect crops from pathogens by: i. avoiding plant stress, which may allow crops to better withstand the attack of pathogens and ii. manipulating nutrients to the advantage of crops and disadvantage of the pathogen. The modern intensive agriculture which mainly relies on chemical inputs (fertilizers and pesticides) and fertilizer responsive HYVs (high yielding varieties) has led the self sufficiency in food production in India and several other countries of the world. But, the indiscriminate and imbalance use of chemical fertilizers along with exhaustive cropping initiated several second generation problems including widespread micronutrient deficiencies and increased attack of several insect-pests and pathogenic diseases. The excessive use of nitrogen and no or reduced application of phosphorus, potash and other micronutrients is creating conducive conditions for several crop pathogens and their insect vectors. This situation frequently results in serious crop losses during favourable environmental conditions for disease development. The specific role of macro and micronutrients in maintaining crop health, effect of their dose and form of application on suppression /induction of various diseases and interaction with other soil/ environmental factor (s) will be discussed in following sections.

Cost is the value of the factors of production used in producing and distributing goods and services. The cost of a factor unit equals the maximum amount which the factor could earn in alternative employment. Broadly speaking, cost is the measure of what has to be given up in order to achieve something. Production costs play an important role in the decision of the farmers. Cost of production here means the exogenesis incurred per unit of output. In general, at given level of prices, a farmer can increase his farm income in two ways i.e. (i) by increasing production or (ii) by reducing the cost of production. The cost of production involves both cash cost and non cash cost items. Cash costs are incurred when resources are purchased and used immediately in the production process such as casual labour, fertilizer fuel etc. And non cash cost like depreciation on farm building and equipment and payment made to farmer himself or family labour, management and owned capital. Before estimating the cost of cultivation/production, we should know the concepts/terms are used in estimating the cost. Concept means idea underlying or general notion.

Cost concepts and items of cost

The cost of production of a crop is considered at three different levels viz., cost A, cost B and cost C. The concept of nine costs such as is followed by the Directorate of Economics and Statistics, Ministry of Agricultures Government of india in their cost studies are given below.

Introduction

Seed spices include group of annuals whose dried fruit or seeds are used as spices. These are characterised by pungency, strong odour, sweet or bitter taste. Coriander, cumin, fennel and fenugreek occupy largest area among the seed spices. However, ajowan, dil, celery, anise, nigella and caraway having secondary place as far as area and production is concerned. Major area under seed spices is in Rajasthan and Gujarat, and other seed spices producing states are Chattisgarh, Madhya Pradesh, Andhra Pradesh, Tamil Nadu and Uttar Pradesh. The seed spices account for about 36% and 17% of the total area and production of spices in country. The export of seed spices from India during 2008-09 was 4.71 thousand tonnes worth Rs. 54914.8 million rupees. The per cent export growth rate during 1995-96, 2000-01, 2005-06, 2007-08 and 2008-09 was 12.0, 11.0, 8.0, 36.0 and 19.0% in terms of value and 13.0, 3.0, 8, 13.0 and 6.0 in respect of quantity, respectively.

Integrated plant nutrient management includes use of organic manure/ compost, bio-fertilizer, chemical fertilizer, green manuring, residue management, legume based cropping system, use of nutrient-responsive varieties, proper method and time oforganic manure and fertilizer application, soil and water management to minimize the nutrient losses occurring through volatilization, denitrification, runoff and leaching. Application of plant nutrients in proper balance form is also a part of Integrated Plant Nutrient Management (IPNS) system. Supply of nutrients to seed spices in appropriate quantities and at the correct times is essential for economically and environmentally sustainable agriculture. Soil organic matter, crop residues and manures play a vital role in the supply of macro and micronutrients and the transformation between the various organic and inorganic forms often control availability, both for plant uptake and loss to the environment (Aishwath and Vashishtha, 2008; Lal, et al., 2009).

Agricultural systems are complex, and understanding this complexity requires systematic research under shrinking agricultural research resources. Field experimentation can only be used to investigate a very limited number of variables under a few site-specific conditions. The crop growth simulation models simulate crop growth, development and yield taking into account the effects of weather, genetics, soil water, carbon and nitrogen, planting, irrigation and fertilizer (e.g. Nitrogen, Phosphorus) management. Ofthe many factors that potentially affect the growth of plants, the two that are most responsive to management are water and nitrogen. It is therefore not surprising that most crop models include routines to handle responses to water and nitrogen. Less frequently do models attempt to describe limitations due to other soil constraints (Probert and Keating, 2000). In the context of integrated nutrient management, the performance of a crop model depends mainly on its ability to adequately describe the release of nutrients from diverse inputs and their uptake by the crop.

Origins and genesis of a crop simulation model

Crop simulation model approach was started near about 50 years ago, in early 1960s when early simple crop models were developed for estimation of transpiration and photosynthesis. The first step towards crop modeling was the development of simple models to estimate light interception and photosynthesis (Loomis and williams, 1963; De wit, 1965 and Duncan et al., 1967). There are many crop growth simulation models. Some are more generic in nature while others are built for specific purposes. Most of these models simulate crop growth and soil processes using daily time steps. All models are developed with some assumptions and hypotheses, and all have strengths, weaknesses and limitations for appropriate application. Well-known crop modelling groups across the world include IBSNAT/IFDC (International Benchmark Sites Network for Agro technology Transfer/International Fertilizer Development Centre) in USA, WAU/AB-DLO (Wageningen Agriculture University/Centre for Agro-biological Research) in the Netherlands, and APSRU (Agriculture Production Systems Research Unit) in Australia. The IBSNAT project was initiated in 1982, and over the past 20 years it has developed more than 15 models, including CERES (Crop Estimation through Resource and Environment Synthesis) Rice and CERES Wheat, which are available either as stand-alone models or within the DSSAT (Decision Support System for Agrotechnology Transfer) shell (Uehara and Tsuji, 1993).

Carbon is found in all living organisms and is the major building block for life on earth. It exists in many forms, predominantly as plant biomass, soil organic matter, and as the gas CO₂ in the atmosphere and dissolved in sea water. Carbon cycles globally among five distinct pools among which the largest oceanic poolis estimated at 38000 Pg (Pg= petagram =1x10¹⁵g=billion tonnes) and is increasingat the rate of 2.3 Pg C yr^-1 (Fig. 1). The geological C pool, comprising fossil fuels, is estimated at 4130 Pg, pedologic, estimated at 2500 Pg to 1m depth, atmospheric pool comprising 760 Pg of C with nearly all of it as CO₂, and the smallest among theglobal C pools is the biotic pool estimated at 560 Pg. The pedologic and biotic C pools together are called the terrestrial C pool estimated at approximately 2860 Pg.

Objective: To identify drought tolerant genotypes by PEG method in laboratory.

Background information:

Among different abiotic stresses, drought is considered to be most detrimental for agricultural production. A drought is a period of below-average precipitation in a given climatic region that results in prolonged shortages in water availability, whether atmospheric, surface water or ground water. A drought can last for several months or years. Drought has substantial impact on ecosystem and agricultural productivity.

Some plant species are better able to tolerate drought stress than others. Genetic variability for drought tolerance also exists within many plant species (Blum, et al. 1980). The efficient exploitation of that variability for crop improvement would be facilitated by a fast and accurate screening method for identifying drought tolerant genotypes (Clark and Mc Craig, 1982). Several methods utilizing seedlings to screen for drought tolerance have been developed using an osmoticum such as polyethylene glycol (PEG) (Singh, et al. 1984). The other synonyms of PEG are Poly ethylene oxide (PEO) and Poly oxyethylene (POE); these other chemicals depend on the molecular weight. Chemical structure of PEG may be written as H”(O”CH₂”CH₂)_n”OH (Kahovec, et al. 2002). PEG is used in certain abiotic stress experiments to produce high degree of osmotic pressure, normally in the order of 'tens of atmosphere' PEG produces osmotic pressure due to its flexible nature and water soluble polymer. PEG also makes certain specific interactions with bio-molecules. Due to above properties PEG is used as a specific chemical to produce osmotic pressure in several biochemical and bio-membrane related experiments. For some genotypes, the seedling response apparently is a good indicator of the plant's reaction to drought stress later in its life cycle. However, early screening of seed plants via seedlings tests must still be considered as a method for identifying genotypes that will tolerate drought stress in the vegetative stage (Andersen, et al. 1987).

Objective: To identify salt tolerant genotypes by Glycine Betaine (GB) method in laboratory.

Background information:

Salt or salinity is a biggest danger that causes reduction in crop productivity worldwide. Salinity reduces the germination percentage, plant vigour and ultimately loss in final crop yield. There are different physiological parameters that are affected by high level of salinity viz., oxidative stress, physical alteration of bio-membrane, nutritional imbalance, loss of cell division & cell expansion, water stress, genotoxicity and ion toxicity. Together, these effects reduce plant growth, development and survival. During the onset and development of salt stress within a plant, all the major processes such as photosynthesis, protein synthesis and energy and lipid metabolism are affected. During initial exposure to salinity, plants experience water stress, which in turn reduces leaf expansion (Petronia, et al. 2011).

The water-soluble compounds which, are low in molecular weight, accumulate at certain concentration known as “compatible solutes” or “osmolytes” is the common strategy adopted by many organisms to combat the abiotic stresses. Some common compatible solutes are betaines, sugars (mannitol, sorbitol and trehalose), polyols, polyamines and amino acid (proline). Their accumulation is favored under water-deficit or salt stress as they provide stress tolerance to cell without interfering cellular machinery. The tolerant or sensitive species show difference in the stress tolerance level based on levels of accumulation of these compounds during abiotic stress condition. Accumulation of osmolytes like glycinebetaine (GB) in cells is known to protect organisms against abiotic stresses via osmoregulation or osmoprotection. Other roles of GB like cellular macromolecule protection and ROS detoxification have been suggested as mechanisms responsible for abiotic stress tolerance. In addition, GB also influences expression of several endogenous genes in plants.

Objective: To estimate total protein in the plant sample by Folin-Lowry's method.

Background information:

Protein assay can be done to determine the level of protein in solution by the Folin-Lowry biochemical method. The concentration of protein leads to change in color of the solution and based on different color in different protein concentration solution the change in color may be digitized by colorimeter or spectrophotometer. This technique was developed by Oliver H. Lowry in the year 1940s. The tyrosine and trytophan amino acid residues in a protein sample produces purple or blue color in the absorption maxima at 660 nm by using Folin-Ciocalteau (having phosphate and sodium tungstate molybdate) chemical reagent. The color intensity of protein sample depends on the concentration of above aromatic amino acids (tyrosine and tryptophan), hence every sample will vary for color. For protein estimation the BSA (Bovine serum albumin) is widely used as standard protein molecule due to its cost effectiveness, purity and easy availability. This method of protein estimation is very sensitive and can detect up to 10 μg/ml. This is a relative method of protein estimation and may be interfere by salts, Tris buffer, nonionic & cationic detergents, EDTA, lipids and carbohydrates. For reproducible assay the incubation time, pH (9-10.5) is critical in this method (Lowry, et al. 1951).

Objective: To separate protein fragments by using SDS-PAGE method.

Background information

Electrophoresis is the motion of dispersed particles relative to a fluid under the effect of limited uniform field of electricity (Dukhin, and Derjaguin, 1974). Ferdin and Frederic Reuss (Reuss, 1809) first time observed such phenomena of electrokinetic nature in 1807. They found that the clay particles move in the water by giving constant supply of current. It happened due to interface of charged particles surface and the surrounding media. The technique is used widely in chemistry, biochemistry and molecular biology to separate different molecules based on difference in charge, binding affinity and size. Electrophoresis may be of two types viz., Anaphoresis and Cataphoresis. Anaphoresis is the electrophoresis of anions (-ve charged ions) while, Cataphoresis is electrophoresis of cations (+ve charged ions). Electrophoresis is a major analytical technique in biochemistry that is used for separation of macromolecules like protein, enzymes and nucleic acids. Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size.

PAGE is abbreviated for Polyacrylamide gel electrophoresis that describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Mobility is a function of the length, conformation and charge of the molecule.

Objective: To extract and perform enzyme assay of polyphenol oxidase from plant.

Background information:

Polyphenol oxidase (PPO or monophenol monooxygenase or polyphenol oxidase I, chloroplastic) is a tetramer that contains four atoms of copper per molecule, and binding sites for two aromatic compounds and oxygen (www.worthington-biochem.com). The enzyme catalyses the o-hydroxylation of monophenol molecules in which the benzene ring contains a single hydroxyl substituent to o-diphenols (phenol molecules containing two hydroxyl substituents). It can also further catalyse the oxidation of o-diphenols to produce o-quinones.

PPO causes the rapid polymerization of o-quinones to produce black, brown or red pigments (polyphenols) that cause fruit browning. The amino acid tyrosine contains a single phenolic ring that may be oxidised by the action of PPOs to form o-quinone. Hence, PPOs are some time referred as tyrosinases (Mayer, 2006).

Jolley et al. (1974) referred to PPO as 4 electron-transferring phenol oxidase and oxygen. It is responsible for browning reactions throughout the phylogenetic scale.

Objective: To extract and perform enzyme assay of catalase from plant.

Background information

Catalase is a commonly distributed enzyme and present in approximately all organisms exposed to oxygen (such as bacteria, plants and animals). Catalysis of H₂O₂ into H₂O and O₂ is done by catalase enzyme (Goodsell, 2004).

Objective: To extract and perform enzyme assay of guaiacol peroxidase from plant.

Background information:

Peroxidases are a large family of enzymes that typically catalyze a reaction of the form:

Objective: To extract and perform enzyme assay of Superoxide dismutase from plant.

Background information:

Superoxide dismutase (SOD, EC 1.15.1.1) is the enzyme that alternately catalyzes the dismutation (or partitioning) of the superoxide (O₂^-) radical into either ordinary molecular oxygen (O₂) or hydrogen peroxide (H₂O₂). Superoxide is produced as a by-product of oxygen metabolism and, if not regulated, may cause cell damage (Hayyan, et al. 2016). Hydrogen peroxide is also responsible for cell damage and may be degraded by other enzymes like catalase. Thus, SOD is an important antioxidant defense in nearly all living cells exposed to oxygen. One exception is Lactobacillus plantarum and related lactobacilli, which use a different mechanism to prevent damage from reactive O₂^-.

Objective: To study peroxidase isozyme variation in rice seedlings by electrophoresis.

Background information

For electrophoresis and PAGE please see practical no. 4. For peroxidase please see practical no. 7.(Page 17-22)

Isozymes (isoenzymes or more generally as multiple forms of enzymes) are the enzymes that are differing in the sequence of amino acids but they work on same substrate or in other words catalyze same biochemical reaction. The isozymes have difference in K_M values and regulatory properties. Isozymes are very useful for proper tuning of metabolic activities and to meet the specific need of particular tissue or developmental stage, e.g. lactate dehydrogenase (LDH). Isozymes are coded by homologous genes that have diversity during evolutionary process. Although, strictly speaking, allozymes represent enzymes from different alleles of the same gene, and isozymes represent enzymes from different genes that process or catalyse the same reaction, the two words are usually used interchangeably.

Objective: To isolate DNA from plant sample by CTAB method and quantification by spectrophotometer.

Background information:

DNA isolation from different plant tissues viz., leaf, stem, root, flower and seed is a challenging task due to hindrance of variety of variety of biochemical compounds present and further problem increases with diverge plant species. In case of animal tissues the diversity in cellular bio-molecules in different species is much lower compared to plants. Two major phyto bio-molecules that have wide variation between species and pose serious problems in DNA isolation are: polyphenols and polysaccharides. These phyto bio-molecules if contaminated in DNA then may hinder further down applications like PCR, restriction digestion, sequencing, etc.

Several methods have been developed and reported that can remove polyphenols and polysaccharides efficiently during DNA isolation from plants. For this purpose two different chemicals are widely used,the first one is CTAB (cetyl trimethyl ammonium bromide) that is a cationic detergent and helps in removal of polysaccharides and the second one is polyvinylpyrrolidone that helps in removal of polyphenols. Due to above properties the CTAB based DNA extraction buffer is very common for plant DNA extraction.

Significance of different types of chemicals found in CTAB based plant DNA extraction method is explained as under:

Objective: To check quality of genomic DNA on agarose gel electrophoresis.

Background information

Agarose gel electrophoresis is a common method of gel based electrophoresis technique used in biochemistry, molecular biology, genetics and clinical chemistry for separation of Protein and nucleic acids from a mixture of macromolecules. Agarose is a major component of agar that is used as a matrix in gel baseelectrophoresis. The nucleic acids (DNA and RNA) are separated based on length of fragments while, proteins are separated based on size and/or charge (Kryndushkin, et al. 2003). For separation of bio-molecules the electric field is applied that leads to movement of charged particles via agarose gel matrix and bio-molecul s are separated based on difference in size in the agarose gel matrix (Sambrook and Russel, 2001).

Gel casting with agarose is easy and has very few charged groups, hence found most suitable for nucleic acid separation in most of the biochemistry, molecular biology and biotechnology laboratories. Separated DNA/RNA ma be seen and recorded with stain under UV light. Further DNA fragments can be eluted from agarose gel with an ease for further down applications. The percentage of agarose gel used is in the range of 0.7 - 2. Lower percent of agarose gel is used for long DNA fragments while, higher percent of agarose gel is used for short DNA fragments with suitable running buffer.

Objective: To amplify DNA using RAPD primers and study on agarose gel.

Background information:

RAPD markers are decamer (10 nucleotide length) DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals. RAPD is applied to analyze the genetic diversity by random primers. Unlike traditional PCR analysis, RAPD does not require any prior DNA sequence information of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions complementary with primers' sequence. For example, no fragment is produced if primers annealed too far apart or 3' ends of the primers are not facing each other. Therefore, if a mutation has occurred in the templ that was earlier complementary with primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. RAPD is a dominant marker means it cannot distinguish between heterozygous; single copy and homozygous; two copies. Rarely RAPD markers may be Co-dominant where different fragment length of DNA is amplified from the single locus. PCR is a molecular technique used for amplification of DNA, a complete enzyme based chemical reaction, therefore, the concentration and quality of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible. Mismatches between the primer and the template may result in the total absence of PCR product or decreased amplified product. Thus, the RAPD results can be difficult to interpret.

Objective:To transfer DNA on nylon membrane by Southern blotting.

Background information

A Southern blot is a method used in molecular biology to detect a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

The British biologist Edwin Southern (Southern, 1975) discovered this technique the technique is known by his name. Southern blotting technique uses digested genomic DNA with restriction enzymes. Southern blotting technique may be used to identify the gene copies number in a genome. A probe that hybridizes only to a single DNA segment that is uncut with restriction enzyme will produce a single band on a Southern blot, whereas multiple bands will likely be observed when the probe hybridizes to several highly similar sequences (e.g., may be due to sequence duplication).

In the southern blotting technique a short DNA probe is used that matches based on complementary DNA sequence fragment on filter membrane. It proves that the DNA fragment on membrane contain specific sequence for which DNA probe is prepared. When DNA is transferred on the membrane from gel it permits binding of radioactive/Biotin labeled hybridization probe to the restricted DNA. Southern blotting is also important for autoradiography.

Southern blotting is an important molecular biology technique that may be used for homology based cloning, screening a DNA library, screening of cloned DNA fragments and to identify methylated sites in genes.

Objective: To isolate RNA from plants using TRIzol reagent and its quantification by spectrophotometer.

Background information

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is necessary in all the steps of protein synthesis in all living cells and carries the genetic information for many viruses.

The isolation of RNA with high quality is a crucial step required to perform various molecular biology experiment. TRIzol Reagent is a ready-to-use reagent used for RNA isolation from cells and tissues (Chomczynski, 1993).TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. Addition of chloroform, after the centrifugation, separates the solution into aqueous and organic phases. Aqueous phase contains RNA.

After transferring the aqueous phase, RNA can be recovered by precipitation with isopropyl alcohol. DNA and proteins can be recovered by sequential separation after the removal of aqueous phase. Ethanol precipitation needs DNA from the interphase, and an additional precipitation with isopropyl alcohol requires proteins from the organic phase. Total RNA extracted by TRIzol Reagent is free from the contamination of protein and DNA The isolated RNA can be further used in Northern blot analysis, in vitro translation, poly (A) selection, RNase protection assay and molecular cloning.

Objective: To check the integrity of isolated RNA on agarose gel.

Background information:

The most common method used to assess the integrity of total RNA is to run RNA sample on agarose gel (denaturing) stained with ethidium bromide (EtBr). While native (non-denaturing) gels can be used, the results can be difficult to interpret. The secondary structure of RNA alters its migration pattern in nativegels so that it will not migrate according to its true size. Nondenaturing conditions also result in bands that are not as sharp, and even multiple bands representing different structures of a single RNA species.

Intact total RNA run on a denaturing gel will have sharp, clear 28S and 18S rRNA bands (eukaryotic samples). The 28S rRNA band should be approximately twice as intense as the 18S rRNA band. This 2:1 ratio (28S:18S) is a good indication that the RNA is completely intact. Partially degraded RNA will have a smeared appearance, will lack the sharp rRNA bands, or will not exhibit the 2:1 ratio of high quality RNA. Completely degraded RNA will appear as a smear (having very low mw). Inclusion of RNA size markers on the gel will determine the band size or smears size and will also serve as a good control for ensuring proper gel run. Note: Poly selected samples will not contain strong rRNA bands and will appear as a smear from approximately 6 kb to 0.5 kb (resulting from the population of mRNAs, and depending on exposure times and conditions), with the area between 1.5 and 2 kb being the most intense (this smear is sometimes apparent in total RNA samples as well).

Objective: To synthesize cDNA from isolated plant total RNA.

Background information:

Complementary DNA (cDNA) is double-stranded DNA made from template of single stranded RNA, [e.g., mRNA or microRNA] by reverse transcriptase enzyme. Mostly cDNA is used for cloning of eukaryotic genes in the prokaryotes. For the expression of a specific protein in a cell where the expression of that protein is not found (i.e., heterologous expression), the cDNA will be transferred that codes for the protein to the recipient cells. Retroviruses (e.g. HIV-1, HIV-2, simian immunodeficiency virus, etc.) also produce cDNA naturally and integrate in host's genome and further creates provirus (Croy, 1998). Major use of cDNA is in gene cloning or as gene probes or in the creation of a cDNA library. When scientists transfer a gene from one cell into another cell for the expression of new genetic material as a protein in the recipient cell, the cDNA will be added to the recipient (rather than the entire gene), because the DNA for an entire gene may have certain DNA that may not code for the protein or that interrupts the coding sequences of the protein (e.g., introns). Partial sequences of cDNAs are often obtained as expressed sequence tags.

Objective: To quantify aflatoxin content in given sample by Indirect competitive ELIS

Background information:

Enzyme linked immuno sorbent assay (ELISA) is a common biochemical technique that uses the principle of solid-phase enzyme immunoassay (EIA) for detection of substances mainly antigens in a liquid or wet sample.

ELISA is a very popular technique that is used for diagnostic purpose in health sector, plant protection and also for quality test in food and other industries. This technique applies the principle of antigen-antibody reaction on a solid surface. The attached antibody is linked to certain enzymes and in the end of reaction by addition of substrate that performs enzyme-substrate reaction and makes product that has some signal mostly color change and based on intensity of color the qualitative as well as quantitative estimation of antigens can be done using ELISA reader coupled with spectrophotometer.

In ELISA technique one antibody should be specific to certain antigen. For reaction of ELISA the ELISA plates are used that is also known as microtiter plate made-up of polystyrene. The sample to be tested for ELISA contain unknown amount of antigen to be detected, this sample containing antigen is immobiliz d on a solid support like polystyrene microtiter plate in two different ways; first is non-specific where antigens adsorbed to the surface and second is specific where the antigens of sample are captured by specific antibody to the antigen, like used in sandwich ELIS Once the antigens are immobilized non-specifically or specifically the detection antibody should be added that makes precise antigen-antibody complex. The detection antibody may be either covalently linked to certain enzyme or may be detected by another secondary antibody linked to bioconjugated enzyme. During ELISA steps several proteins or antibodies may non-specifically bound that can be removed by washing with mild detergent solution in between of each ELISA step. For washing of ELISA plates, ELISA plate washer may be used that mechanically wash the ELISA plates. At the end of ELISA the final wash is given and substrate is added that produces visible signal in the form of color change that indicates the presence of antigens in the sample and further quantification may be done using ELISA reader coupled with spectrophotometer.

1.Which one is the example of abiotic stress?

a.Drought

b.Salinity

c.Flood

d.All of these

2.Which one is the example of osmoticum?

a.NaCl

b.PEG

c.H₂O

1. Introduction

Vedic system of medicine, also called Hindu medicine, Aryan median, Indian system of medicine and in modern era as science of life (Ayurveda). It traces origin from mythical, through a semi-mythical, to historical beginning. Clues on uses of medicine are although obtainable since Indus civilization, it was propounded more in the Vedic period and also in post Vedic era. It is claimed to be the oldest medicinal system and heritage of India. The ancient Indians were first to cultivate astronomical science, mathematics, chemistry, grammar, lexicography, architecture and Dhanur-veda. Another compartment of human life is medicine, in which Indians never lagged behind. Vedic medicine is not born within four walls of a laboratory. It is nurtured in nature, human minds and hearts. The Vedic wisdom is engendered by trial and errors, experimentation, natural observations and timely criticism by the learned exponents. Obviously, there is no denial to the fact that the torch of learning was handed down to the other civilizations by the ancient Indians. Vedic medicine is not confined to the boundary of Indian nation but percolated in other cultures. Careful and critical examination of such a great heritage is a task of not of ordinary men. Still I took interest to review it, although almost impossible to justify it within the limits of few pages and time. The title of my paper amply suggests that Vedic medicine is a mine of information but I have tried to put on record it, in a nutshell. To tap down information in historical perspective, relevant literature has been borrowed and consulted to pinpoint the events, concepts wisdom of ancient Indians. These literary sources are enlisted under references.

1. Introduction

Plant is probably the greatest gift of nature. Plants provide us with food, cloth, house, papers and many others. Some of the plants have medicinal values and are known as ‘medicinal plants’. Our ancestors used these plants not only for protection from diseases but also for the treatment of several ailments. China has the highest number of the medicinal plants used (about 11,500 species) followed by India (about 7500 species). People of other countries also use medicinal plants in varying amount (Rafieian-Kopaei, 2013). Tradition is still going on. It is reported that even today 80% of the world population relies on medicinal plants in their medication (Hamilton, 2004). Few plants secrete essential oils full of aroma and are called ‘aromatic plants’. These plants are being utilized in industry for the production of essence and many other products. Medicinal and aromatic plants (MAPs), therefore, are in continuous use from past to present. In future also there will be tremendous scope of utilization of MAPs in drug discovery, aromatherapy, as cosmeceuticals, nutraceuticals as well as in animal husbandry. Present review will focus utilization of Medicinal and Aromatic Plants (MAPs) in past and present and its possible prospect in future.

1. Introduction

Man (Homo sapiens) since his appearance on the sylvan Blue Planet started his search for healthcare and beautycare, besides food and shelter. Eventually, he also became conscious about his mind and attempted at ‘mindcare’. He employed leaves, flowers, fruits, etc. or some plant products altering his mind to remain aloof from his heavy worldly affairs, stress or even incurable diseases. He also endeavoured to use plants for his amusement. He exerted more pressure on local biodiversity, modified his study and observations on biodiversity which resulted into ‘cultural biodiversity’. He intensified his method of ‘trial and errors’ to search new sources or biodynamic chemicals. In recent times, he is emphasizing value-addition of the products in hand for his welfare. After food, medicine was his next priority to sustain life in primitive societies. Naturally, his study and research laid emphasis more on food and medicine in comparison to mind-altering bioresources. Even the modern man also paid more attention on the former rather that the latter. These trends have perforce led in partial negligence of research for psychoactive plants in his ambience. The present author, therefore, extended efforts to prepare an inventory of the mind-altering vis-à-vis psychoactive plants in the state of Maharashtra.

1. Introduction

The global population is very cautious about gaining weight and diabetics. Calorie less and sugar free substances are gaining momentum in food sector. The miracle plant stevia is very fit in this scenario as it is both zero calorie and sugar free and one more additional benefit that it is completely natural. Stevia rebaudiana Bert. belonging to the family asteraceae has been used in many countries for thousands of years as a source of natural sweetener eg., in Paraguay and Brazil Central America, Thailand, Korea and China (Megeji et al., 2005, Sivaram and Mukundan, 2003; Jain et al., 2009). It is also known as sweet herb, sweet leaf, honey leaf, candy leaf, honey yerba (Carakostas et al., 2008) and meeti tulsi. It possesses an extensive root system and brittle stems producing small, elliptic leaves. In natural conditions S. rebaudiana grows in a form of a shrub and reaches even 1 m of height. Leaves are the major economic part. They are sessile and oppositely arranged, lanceolate to oblancoelate in shape, and are serrated above the middle. Trichome structures on the leaf surface are of two different sizes, one large and one small (Shaffert and Chetobar 1994). The white florets are, borne in small corymbs of 2–6 in number. Corymbs are arranged in panicles. Dr. Moises Santiago Bertoni discovered this plant in 1888 at Paraguay. In 1905, the plant was scientifically named as S. rebaudiana after a Paraguayan chemist Dr. Rebaudi. (Gupta et al., 2013). The active components of Stevia i.e., steviol glycosides (SGs) includes rebaudioside A (Reb A), rebaudioside C and stevioside (ST) that are 250-300 times sweeter than sugar (Chalapathi & Thimmegowda 1997). Stevia is a short-day plant and its flowering is induced when days are shorter. Long photoperiods stimulates leaf growth and steviol glycoside content. The plant can survive only mild winters of its native regions. It cannot survive in waterlogged areas. Stevia extracts can be used in a wide variety of food and beverage applications. Individual usages and use levels may vary depending on the country. They act synergistically when used in combination with other sweeteners. The higher market demand favors the cultivation and commercialization of this particular species.

1. Introduction

The Aquilaria malaccensis (Agarwood) is an Appendix II species of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES, 1994). The species is also red listed under International Union for Conservation of Nature and Natural Resources (IUCN) and is classified as ‘Vulnerable’ globally but ‘Critically Endangered’ in India (Oldfield et al., 1998; IUCN, 2009) and almost ‘Extinct in wild’ in Assam (Anon., 2003; Saikia and Khan, 2012a). The species due to its multipurpose uses is overexploited which is threatening its existence from the wild (Lee and Mohamed, 2016; Talucder et al.,2016). There are 21 reported Aquilaria species, of which 13 are fragrant resin producers, while the rest are yet to be studied (Lee and Mohamed, 2016; Talucder et al.,2016). Eleven of the domesticated species are A. malaccensis, A. crassna, A. subintegra, A. hirta, A.rostrata, A. beccariana, A. filaria, A. khasiana, A.microcarpa, A. grandiflora and A. sinensis (Zuhaidi, 2016). Species reported from Asia viz., A. apiculata, A. baillonii, A. banaense, A. beccariana, A. brachyantha, A. cumingiana, A. filaria, A. hirta, A. khasiana, A. malaccensis, A. microcarpa, A. rostrata, A. sinensis, A. subintegra and A. crassna are known to produce essential oils (Sulaiman et al., 2015). Only three species i.e. Aquilaria khasiana, A. macrophylla and A. malaccensis has centre of origin in India with A. macrophylla endemic to Nicobar Islands (Anon., 2003; Giri, 2003) and A. khasiana endemic to Khasi Hills of Meghalaya and surroundings (Kanjilal et al., 1982), while A. malaccensis is native to evergreen rain forests in the foothills of northeastern India (Chakrabarty et al., 1994; Saikia and Khan, 2012b) and West Bengal (Barden et al., 2000).

1. Introduction

Medicinal plants are used to be the valuable sources of new drugs (Nalawade et al., 2003; Chen et al., 2010;Chacko et al., 2010; Hamilton 2004). In Europe, about 1300 plants were used as medicinal plant, of which 90% are collected from wild resources. In United States, about 118 out of 150 prescribed drugs made up of natural sources (Balunas and Kinghorn 2005)

Moreover in developing countries, about 80 % people are totally reliant on herbal drugs and over 25 % of prescribed medicines derivative from wild plant species (Hamilton 2004). With the increasing demand for herbal drugs, natural health products, and secondary metabolites of medicinal plants, the use of medicinal plants is growing rapidly throughout the world (Nalawade et al., 2003). Between 50,000 and 80,000 flowering plant species are using for medicinal purposes worldwide according to the International Union for Conservation of Nature and the World Wildlife Fund (Bentley 2010). However, 20 % of the wild resources have already been exhausted with the increasing human population (Ross, 2005). Worldwide loss of species and habitat destruction has increased the extinction rate of medicinal plants, especially in China (Nalawade et al., 2003; Heywood and Iriondo, 2003), India (Heywood and Iriondo, 2003; Hamilton, 2008), Nepal (Hamilton, 2008), Kenya (Hamilton, 2008), Uganda (Zerabruk and Yirga, 2012) and Tanzania (Zerabruk and Yirga, 2012). China and India used highest numbers of medicinal plants i.e. 11,146 and 7500 species respectively (Rafieian-Kopaei, 2013). So, it is now necessary to find out new medicinally important plants or to utilize the well known medicinal plant sustainably. There are many plants in India that contains different medicinal and nutritional components, which can be exploited much more efficiently to establish a new era of medical science.

1. Introduction

There are a large number of indigenous rice varieties in India, which are still grown by the tribal people and small farmers of the remote areas where the modern agricultural practices, sufficient foods as well as healthcare systems are a dream. Nature has provided them some alternative ways. They have different indigenous rice varieties with its nutritional and medicinal values. Until recently, rice was considered only a starchy food and a source of carbohydrates and some amount of protein. Rice protein, though small in amount, is of high nutritional value (Chaudhary and Tran, 2001). It has extensive curative properties known from the ancient days. In fact, specific rice varieties with medicinal properties were cultivated and used in the treatment of some ailments in different counties of South East Asia.

During recent times, both at national level and international levels, the land races are being preserved in the gene banks. The scientists said that they were also checking these land races for some such characteristics which were not available in the existing varieties so as to incorporate the same in the present varieties (Sultan and Subba Rao, 2013). Traditional varieties are experiencing an increasing trend among consumers, due to their incredible health benefits for example the traditional varieties possess higher amount of glutamic acid, fiber and vitamins. Some people also credit traditional varieties with other health benefits, such as giving sensations of cooling in the body, improves vocal clarity, eyesight, fertility and mitigating rashes (Caius, 1999).

While reviewing the status of education and research on medicinal plants in Bangladesh, it was found that there are 27 government and private educational institutions currently engaged in offering Bachelor or Diploma degree on Unani and Ayurvedic systems and training of traditional medicine practitioners in Bangladesh. Several other public and private universities have included different aspects of medicinal plants in their syllabus with other degrees at undergraduate and post graduate level. At present, there are 277 Unani, 201 Ayurvedic and 32 herbal drug manufacturers in Bangladesh with 6498 Unani, 4058 Ayurvedic and 537 herbal products registered by the Directorate General of Drug Administration. About 500 species of plants are identified which possess medicinal properties and are distributed throughout the country. There is no distinctive research organizations at present for conducting planned research solely on medicinal plants; though, the Bangladesh Forest Research Institute have some research programme on medicinal plants. Hamdard University- a private university is offering degree on Unani and Ayurvedic systems and conducting some research including drug manufacturing. Bangladesh Agricultural Research Institute with other public and private universities are conducting some hectic research on medicinal plants including some high yielding variety development. The Government of Bangladesh (GoB) have taken some initiatives for development of Unani and Ayurvedic medicine sector like making ‘Bangladesh Unani and Ayurvedic Practitioners Ordinance, 1983’, ‘National Drug Policy 2016’, establishment of ‘Board of Unani and Ayurvedic Systems of Medicines (BUASM)’, etc. But proper implementation of the ordnance, strengthening the activities of BUASM and taking organized research programme on medicinal plants are very much essential for having skilled professionals, quality drugs and research.

1. Introduction

Pummelo is considered as largest fruit among all the Citrus species from the Rutaceae family. It is also known as shaddock, papanus or chakotra. Although C. grandis (L.) Osbeck is more frequently used, however C. maxima (Burm.) Merr. is considered as correct under the International Code of Botanical Nomenclature (Singh and Navneet, 2017). It is considered as one of the four true species (natural, non-hybrid) of Citrus. The ltypical large size, thick rind is one of the causes for slow acceptance of pummelo in international market. However, it is presently considered as important fruit due to presence of various bio-active compounds related with human health and nutrition. The word ‘pummelo’ was originally derived from the Dutch word ‘pompelmoes’. The fruit is also named as ‘Shaddock’ after a Captain Shaddock who was believed to have introduced the fruit in Barbados on an East India Company ship on its way to England in the 17^th century. It is known by different names by different communities residing in India such as chakotra (Hindi), batabi lebu or jambura (Bengali), robab tenga (Assamese), nobab (Manipuri), ser trawk (Mizo), soh myngor or soh bah (Khasi), rabab tasing (Adi), papanas (Gujarati), chakota (Kannada), kambili naranga (Malayalam), pamparapanasa (Telugu), metukki or pampalimaku (Tamil) and bampara (Marathi) [Anon, 2019]. Pummelo can be distinguished from grapefruit by its larger size fruit, thicker albedo and the bunch bearing habit of grapefruit can also be considered for distinguishing it from pummelo (Scora et al., 1982). The crop is believed to have originated from South and Southeast Asia and it is now cultivated in many tropical and semi tropical countries. The top ten world producers of pummelo and grapefruit are USA, China, Mexio, South Africa, India, Argentina, Turkey, Cuba, Brazil and Tunisia (FAOSTAT, 2010). It is also cultivated in in Bangladesh, Cambodia, Chile, Philippines, Vietnam, Sri Lanka and Nepal. There is no report of its commercial cultivation in India till date; rather it is mainly grown in homestate gardens across the country including UP, Punjab, Maharashtra, Tamil Nadu and Karnataka. However, maximum genetic diversity in pummelo germplasm is found in North Eastern Region, West Bengal and Bihar (Singh et al., 2015).

1. Introduction

The interest in the uses of medicinal plants has grown considerably from the mid of 20^th century with the introduction of phytochemical analysis of natural products from various plants parts (Matos, 1996). Most studies survey specific local communities to examine patterns of use of plant part for treating various aliments along with the influence of economic and social variables on plant use (Albuquerque, 2006). Medicinal plants are the base for the development of new drug, which is the basic mode of survival of human and livestock from many diseases to maintain their healthcare (Uddin et al., 2014). Number of studies has been carried out globally on ethnomedicinal uses of plants among various indigenous communities (Alexiades and Sheldon, 1996; Basualdo et al., 1995; Martin, 1995). Particularly from the region Karbi Anglong, Assam (Teron and Borthakur, 2012, 2013; Terangpiet al., 2014; Teronpi et al., 2015) have also conducted many notable work on ethnomedicinal. While in all these studies qualitative approaches have not been adapted to document ethnobotanical information. The quantitative approaches on ethnobotany have grown steadily in the last two decades. Today researchers have developed and applied quantitative methods to the various ethnobotanical data to test different hypotheses about the relation between plant species and humans (Reyes-García et al., 2006). Quantitative ethnobotanical indices were applied to determine how important these plants are to ethnic and indigenous cultures whether, as food, veterinary medicine, human medicine or economic value from various non-timber forest products (Pieroni, 2001; Upadhyay et al., 2011; Reyes-García et al., 2006). Therefore, the aim of this study was to document the various uses of the medicinal plant Geophila repens (L.) I.M. Johnst by the Karbi tribe and to apply quantitative methods to determine the specific importance of these plants for treating particular ailment. G. repens is a very rare plant with high medicinal potential (Roy et al., 2011) and is global distribution is reported from India (Assam, Meghalaya, Andaman Islands and Western Peninsula); Bangladesh; Bhutan; Sri Lanka; Nepal; Malaysia; South China; Fiji; Tropical America; and Africa.

1. Introduction

Jackfruit (Artocarpus heterophyllus) is one of the most significant trees in tropical and subtropical homegardens and perhaps the most widespread and useful tree in the important genus Artocarpus. Jackfruit belongs to the family Moraceae. The word “jackfruit” comes from Portuguese jaca, which is derived from the Malayalam language term, Chakka. Jack fruit native to India and seen abundant in Western Ghats (Wangchu et al.,2013). Besides India, jackfruit is commonly grown in home gardens of tropical and sub-tropical countries especially Sri Lanka; Bangladesh, Burma, Philippines, Indonesia, Thailand, Malaysia and Brazil. In India, it widely distributed in the states of Assam, West Bengal, Uttar Pradesh, Maharashtra, Kerala, Tamil Nadu and Karnataka and considered to be the “Poor mans food” (Prakash et al., 2009). In Malayalam, jack fruit is called as “Chakka” while the ancient Indian language Sanskrit refers as Atibruhatphala (Baliga et al., 2011). The morphology of the tree varies with 10-30 m tall; with long tap root and dense crown producing the largest tree born fruit in the world. The fruit weight up to 50 kg, but average weigh is considered to be 10 kg, while only 30-35% of the bulb is edible. Jack fruit is considered as national fruit in Bangladesh and highly appreciated in India due to cheap and availability in summer seasons were food is scarce (Muralidharan et al., 1997). It is a monoecious tree and both male and female inflorescences are found on the same tree. The fertilization is by cross-pollination and the propagation is mostly through seeds. The complete fruit development process takes about three to seven months from the pollination, varying in different countries. Jackfruit consists of lower fleshy edible region (bulb), middle fused region (syncarp) and out spiney region (spike). When ripe the fruit get fleshy, outer spines widened and flesh get soft and yellow. Except the thorny outer bark and axis are not edible (Baliga et al., 2011). The jackfruits were classified based on their phonotypical and organoleptic characteristics with variation in bulb colour as well as shape, size, odour, flake size, flake colour and period of maturity. Two types of ecotypes are recognized, one with soft and spongy flakes or bulbs while other with firm carpels.

Dioscorea L. commonly known as yam is the largest genus in the family Dioscoreaceae with 602 species distributed in the tropical and subtropical regions. It is an important food crop next to cereals and grains due to high yield storage of carbohydrates which form an important place in the dietary habits of small and marginal farmers especially in the food security of tribal population. The tubers are rich source of various secondary metabolites like Diosgenin, a commercially important bioactive sapogenin used as a precursor in the manufacturing of sex hormones, oral contraceptives and other pharmaceutically important steroidal drugs. Despite of its huge diversity only few species are recognized to be useful for medicinal and edible purposes. About 50 species of Dioscorea are distributed in India. The North East India, one of the hotspot of biodiversity is particularly rich in tropical root and tuber crops. Dioscorea species are in luxuriant growth in wild habitat in this region. The present study mainly addressed to explore the ethno medicinal uses, phytochemical analysis and also focused on safe conservation of sixteen species of Dioscorea found in this region.

1. Introduction

The North east India (located between 87Ú32’E to 97Ú52’E latitude and 21Ú34’N to 29Ú50’ N latitude) forms a distinct part of the Indo-Burma hotspot which rank the 6th among 25 biodiversity hotspot of the world is climatically, ecologically and ethnically very diverse. The region is largely dominated by tribals such as Naga, Khasi, Mizo, Miri, Adi, Aka, Apatani, Maripa, Mushard, Garo, Tagin Naga and Singhpho who are sociocultural different. The North east region, besides having large floral biodiversity, is yet not fully explored. The forest resources available in this area are most often ill managed due to lack of awareness and anthropogenic activities. Various wild roots and tuber crops are consumed by the indigenous group of North East India which have very less scientific documentation although. As wild tuber crops available in North East India, Dioscorea species play a prime role in providing food and medicinal requirements for the indigenous communities of this region. Hence the present paper focuses on the ethnobotanical importance and qualitative screening of few important phytochemicals present in the various species of Dioscorea, their conservation strategies and sustainable utilization in North East India.

1. Introduction

Valeriana jatamansi Jones is considered to be highly endangered medicinal plant found mainly in northern part of Himalayan region. Although it is also found in all temperate regions of world except in Australia (Jain, 1968; Polunin and Stainton, 1987; Bennet, 1987). It is grown in Himachal Pradesh, northern part of U.P., Sikkim and newly introduced in West Bengal Darjeeling region by us. It is enlisted in the endangered category by IUCN (Kaul and Honda, 2000) and it is enlisted as an endangered species in the national medicinal plant board, New Delhi (www.nmbp.nic.in). The plant was reported to be found in fragmented populations in different places of Kashmir Himalayan hilly region like Dara, Sonamarg, Harwan, Gulmarg, Ferozpur, Pahelgum, Yusmarg, Shajnar (Naqashi and Dar, 1982-86). It is found to be in the margin of extinction from the world though it is widespread in the temperate regions of whole world except Australia (Nayar and Sastri, 1998). Indiscriminate collection of rhizomes from the plants made potential threat of its existence in some previously founded localities (Wyatt, 1981; Nayar and Sastri, 1998; Airi et al, 2000; Nautiyal et al, 2003, CAMP, 2003).

Ethnobotany deals with different aspects of man-plant relationship with special emphasis on ethnic community of a region, nay country or any geographical territory. This perhaps gives us a new dimension, orientation and objectives to make it complete with vivid information on people and their resource use pattern including science background behind scientific use. The new form of ethno-botany is now reformed and re-oriented as beta-ethnobotany. Earlier taxonomists, anthropologists and social workers have made their work on a wide field of man-plant relationship which was very loose in the sense that no specification or special focus was there which has its origin so called alpha-ethnobotany. Beta-ethnobotany deals with objective based oriented studies, divine, dynamic and quantitative ethno-botany with special emphasis to assessment of credibility of folk claims, ethno pharmacology, ethno-pharmacotherapy and ethno-medicine. Presently scientists estimated that out of over 5000 plants have been used in ethno-medicine much less than 1000 are utilized in herbal drug industry in different indigenous systems of medicine like ayurveda, homoeopathy, unani and siddha. A wide variation of use and conservation strategies on that medicine or the raw sources of these materials are available in the lower tract of Chotanagpur plateau in red lateritic part of West Bengal so called the ‘ rarh’ region. Tribal people like santal, kol, sabar, kheria, oraon, munda etc. inhabiting the rarh region use the medicine as ethno-medicine since time immemorial and conserve the plants and similar plant propagules in their home gardens and nearby sal (Shorea robusta) dominated forests for future generation. Wide application and conservation strategies of a large number of ethno-medicine and ethno-medicinal plants have been presented here with special emphasis on landscape conservation to make the ecosystem pristine rather than degraded in near future.

This study deals with the ethnomedicinal prospects of common weeds thriving in the wide array of terraced paddy fields used for rhizipisciculture by the Apatanis, a unique tribe indigenous to the remote Ziro valley in the Eastern Himalayas. Surveys were conducted in agricultural fields surrounding three villages in order to record the paddy field flora present on the partition bunds. Elderly villagers were also interviewed with the help of an interpreter to document their traditional knowledge about the medicinal uses of these weeds. A total number of 36 species of indigenous medicinal plants were recorded along with the local name, the plant parts used, the modes of preparation and administration of traditional herbal remedies and the illnesses treated. This easily accessible medicinal plant resource, ameliorating a wide range of common ailments, was found to be a reliable means for managing the primary healthcare of this indigenous agrarian community of the Eastern Himalayas.

For the first time, the Quality Planting Material (QPM) production from aerial stem node cutting and its comprehensive growth parameters are being reported in Valeriana jatamansi (Jones) (family: Valerianaceae), a highly endangered medicinal plant and a natural habitat in North Eastern Himalayan Region in the high altitude ranging from 1000 to 8000m Above Sea Level (ASL). The present communication identified a novel avenue exploring the unused plant part, aerial node cutting for producing QPM in a continuous and exponential rate without scarifying the rhizome and root, enriched with active ingredients like Valerenic acid and Valepotriates. This method also assures 100% plant emergence and more than 91±5.56% plant survival in the different field conditions without requiring hardening process as an additional merit. This first-time report for QPM production of an endangered medicinal plant, Valeriana jatamansi through aerial node cutting without following the hormone supplemented MS based tissue culture, a high cost process required sophisticated instruments and aseptic environment. This present process will have potentiality to explore commercially for mass clonal-propagation even at farmer level.

1. Introduction

Cucurbits are group of plants belongs to family Cucurbitaceae which are mostly creeping or crawling in nature and distributed in tropical and subtropical zone of the globe. The family cucurbitaceae has around 120 genera and more than 800 species (Swarup, 2016). It holds the highest rank amongst all other plant families considering number and percentage of species used for human consumption either as fruit of the tender leaves and stems. Cucurbits have diverse daily use such as salad (cucumber and long melon); dessert (watermelon and muskmelon), curry or boil (bottle gourd, bitter gourd, sponge gourd, ridge gourd, summer squash, squash melon, pumpkin etc.); candy (wax gourd); pickles (pickling cucumber, pointed gourd); jam (pumpkin); utensil (bottle gourd), musical instruments (bottle gourd). From plant breeding point of view, the crops are cross pollinated and entomophilies.