eChapters

Objective: To study peroxidase isozyme variation in rice seedlings by electrophoresis.

Background information

For electrophoresis and PAGE please see practical no. 4. For peroxidase please see practical no. 7.(Page 17-22)

Isozymes (isoenzymes or more generally as multiple forms of enzymes) are the enzymes that are differing in the sequence of amino acids but they work on same substrate or in other words catalyze same biochemical reaction. The isozymes have difference in K_M values and regulatory properties. Isozymes are very useful for proper tuning of metabolic activities and to meet the specific need of particular tissue or developmental stage, e.g. lactate dehydrogenase (LDH). Isozymes are coded by homologous genes that have diversity during evolutionary process. Although, strictly speaking, allozymes represent enzymes from different alleles of the same gene, and isozymes represent enzymes from different genes that process or catalyse the same reaction, the two words are usually used interchangeably.

Vegetable is an important constituent of Indian diet. India is the second largest producer of vegetables which contributes 16.7% to global vegetable area with a production of 156.33 million tons (15.4%) (Table 1). The level of productivity of vegetable17.4 tons/ha is quite low (Rai et al., 2014). Majority of Indians are vegetarian, with a per capita consumption 135 g per day as against the recommended 300 g per day. It is still very less than recommended diet level. In near future, there is a need of around 5-6 million tons of food to feed our 1.3 billion Indian population expected by the year 2020 (Paroda 1999). The major biotic stress in vegetable production is due to increased insect pest. In many cases, there is 100 per cent yield loss due to viral diseases vectored by insects. Vegetables are more prone to insect pests and diseases mainly due to their tenderness and softness as compared to other crops and virtual absence of resistance characters because of intensive hybrid cultivation. The insect pests inflict crop losses to the tune of 10-30 per cent in vegetable production (Table 2). To overcome these losses farmers used indiscriminate doses of toxic chemical on vegetable. It accounts for 13-14 per cent of total pesticides consumption, as against 2.6 per cent of cropped area (Sardana, 2005). For the benefit of the farmer and other, application of BIPM is must. Bio intensive pest management involves every part of management that keeps pest below economic injury level so that pest does not cause any economic loss to the farmers or growers.

Poultry industry in India has registered a phenomenal growth during the last four decades making it one among the world leaders in poultry production. The development of organized poultry has in fact masked the contribution of backyard poultry or house-hold poultry of rural sector. Backyard poultry production in India is characterized by small flock size consisting of 5-10 predominantly non-descript birds maintained in extensive system under zero input conditions but fetch the owners much needed animal protein and supplementary income. Importance of backyard poultry is well recognized by Government of India and special programmes are formulated for its promotion.

Non-descript Chicken

Maintaining small flocks of local non-descript fowls under free range condition by landless poor, small and marginal farmers are a common sight in rural areas. These birds are very popular due to their adaptability to local agro climatic conditions and management practices with prominent brooding behaviour and mothering ability. But these birds are small in size, poor layers (40-50 eggs/ annum), with small clutch size and intense brooding behavior, thus making them not suitable for commercial exploitation.

Starting from the middle of the 20^th Century, information has become a commodity. Those best able to access information will be more successful than their peers in their future careers. Technological competence will be as important as medical competence to practice at the highest levels. Advances in computer technology have made access to veterinary and medical databases much simpler than in the past. It is quite likely that in the future, it will be a malpractice for practitioners not to access online information essential to the treatment of their patients. Wireless digital transfer of information will make access to necessary information independent of time and place.

Veterinary medicine and educational delivery are becoming an increasingly international market. Those who are prepared to deliver material in this manner will have a competitive advantage. Use of digital technologies will allow veterinary colleges to share information, including class material, more efficiently. This will enable each college to market specific educational information for students at other veterinary colleges or continuing education modules to graduate veterinarians around the world. On-line certificate programmes in specific veterinary topics will be developed. Use of computer technologies can enrich the teaching environment. By digitizing library, classroom, and laboratory visual images, students can access these materials at any time or place, freeing them from library and classroom schedules. Medical colleges have found that students heavily use these image resources. Computer technologies provide both horizontal and vertical integration of the professional curriculum, allowing students to explore information to the depth at which they are comfortable, without regard to their “year of study” in a traditional “linear” curriculum. Allowing students to see future applications of current learning material provides additional incentive to master basic material. Computers are a tool, just like a textbook, notebook, or calculator.

7.1. Rural Poverty in Nigeria

The Federal Republic of Nigeria has a population of more than 160 million - the largest in Africa - and a fast-growing economy. Agriculture is the mainstay of the economy, contributing about 40 per cent of GDP. The agriculture sector employs approximately two-thirds of the country’s total labour force and provides a livelihood for about 90 per cent of the rural population. Nigeria is the world’s largest producer of cassava, yam and cowpea - all staple foods in sub-Saharan Africa. It is also a major producer of fish. Yet it is a food-deficit nation and imports large amounts of grain, livestock products and fish. Nigeria’s huge agricultural resource base offers great potential for growth. Recent government policies have started to show results: The agricultural sector reportedly grew by 7 per cent a year between 2003 and 2007, and at a slightly lower rate in recent years. Still, the area of land under cultivation could be doubled. Of an estimated 71 million hectares of arable land, only about half is presently under production. And there is substantial scope for an increase in irrigation, which now covers only 7 per cent of irrigable land. Irrigation and other inputs would substantially increase average yields for major staple crops, which are below those in other developing countries.

Despite Nigeria’s plentiful agricultural resources and oil wealth, poverty is widespread in the country and has increased since the late 1990s. Some 70 per cent of Nigerians live on less than US$1.25 a day. Poverty is especially severe in rural areas, where up to 80 per cent of the population lives below the poverty line, and social services and infrastructure are limited. The country’s poor rural women and men depend on agriculture for food and income. About 90 per cent of Nigeria’s food is produced by small-scale farmers who cultivate small plots of land and depend on rainfall rather than irrigation systems. The poorest groups eke out a subsistence living but often go short of food, particularly during the pre-harvest period. The productivity of the rural population is also hindered by ill health, particularly HIV/AIDS, tuberculosis and malaria.

Introduction

Oil palm is recognized as the highest and cheapest edible oil-yielding crop. Palm oil is extracted from the fruit’s fleshy mesocarp, which contains 45-55 percent oil. The crop is unique in producing 2 distinct types of oil palm oil and palm kernel oil, which are used for culinary and industrial purposes. Being rich in beta carotene, palm oil contributes substantially to the nutritional and energy requirements of the masses. It is also grown in India Andhra Pradesh, Karnataka, Tamil Nadu, Mizorum, Orissa.

India has experienced rapid urbanization as well as industrialization since independence. Though, the country has achieved considerable progress in industrial growth as well as expansion of urban and town spread, the land available in urban periphery as well as near big rivers are being utilized for non agriculture purposes. Similarly, for the development of road, rail as well as irrigation infrastructure, the government reserved huge land, which was under cultivation of various crops. The non agricultural businesses became more remunerative near cities and has increased the value of land near cities; which is not affordable for agriculture production. Considering the limitation of land for production purposes in urban areas, the concept of Urban Horticulture was conceived.

Mycotoxins are naturally occurring toxic secondary metabolites produced by molds. More than 25% of world’s food crops are contaminated with mycotoxins (Eskola et al., 2020). Like other environmental pollutants, mycotoxins also harm the health and productivity of animals and poultry. Mycotoxin development typically occurs in the field, during feed processing and storage under unfavourable conditions. Factors such as temperature changes, storage time, and conditions play a crucial role in fungal growth and aflatoxin synthesis.

Bovine
 
1.    PPR    Eye, mouth, rectal swab in PBS on ice, 10 ml blood  in anti-coagulant at height of temperature, Prescapular lymph node, spleen, piece of intestine on ice, lung, liver, spleen, tonsil in 10% neutral formol saline solution.

Objective: To isolate DNA from plant sample by CTAB method and quantification by spectrophotometer.

Background information:

DNA isolation from different plant tissues viz., leaf, stem, root, flower and seed is a challenging task due to hindrance of variety of variety of biochemical compounds present and further problem increases with diverge plant species. In case of animal tissues the diversity in cellular bio-molecules in different species is much lower compared to plants. Two major phyto bio-molecules that have wide variation between species and pose serious problems in DNA isolation are: polyphenols and polysaccharides. These phyto bio-molecules if contaminated in DNA then may hinder further down applications like PCR, restriction digestion, sequencing, etc.

Several methods have been developed and reported that can remove polyphenols and polysaccharides efficiently during DNA isolation from plants. For this purpose two different chemicals are widely used,the first one is CTAB (cetyl trimethyl ammonium bromide) that is a cationic detergent and helps in removal of polysaccharides and the second one is polyvinylpyrrolidone that helps in removal of polyphenols. Due to above properties the CTAB based DNA extraction buffer is very common for plant DNA extraction.

Significance of different types of chemicals found in CTAB based plant DNA extraction method is explained as under:

Mixed farming exists in many forms depending on external and internal factors. External factors are weather patterns, market prices, technological developments, etc. Internal factors relate to local soil characteristics, composition of the family and farmers’ ingenuity. Farmers can decide to opt for mixed enterprises when they want to save resources by interchanging them on the farm - because these permit wider crop rotations and thus reduce dependence on chemicals, because they consider mixed systems closer to nature, or because they allow diversification for better risk management. There is wide variation in mixed systems. Even pastoralists practice a form of mixed farming since their livelihood depends on the management of different feed resources and animal species. Mixed farms are systems that consist of different parts, which together should act as a whole. They thus need to be studied in their entirety and not as separate parts in order to understand the system and the factors that drive farmers and influence their decisions. It may be the most important principle to achieve increased production in mixed systems, together with the awareness that crops and animals have multiple functions.

IDENTIFICATION OF ANIMALS

It has been in practice since long, not recent item in livestock farming. In earlier day’s in Egyptian era, people used to mark the animal to identify their animals. But in recent days, due to development in bio-medical science where usage of animal has increased within turn has become necessary to establish a suitable identification method for various species of animal. There are many methods available to identified animal, however the methods employed should be simple, economical and easy to identify animal and should be harmless to animal, quickly applicable and should be suitable to experiment conducted.

Computers take on an increasingly important role in today’s world. In this regard, their utility as tools for imparting high quality professional knowledge to veterinarians is much needed. e-learning covers a wide set of applications and processes, including computer-based learning, web-based learning, virtual classrooms, and digital collaboration. The delivery of content could be via electronic media, including the internet, intranets, extranets, satellite broadcast, audio/video tape, TV and CD-ROM. Information and Communication Technology (ICT) in the form of on-line teaching, face-to face video-cases and video-performances has recently been applied in the veterinary profession all over the world. ‘m-learning’ is the new buzz word almost indicating learning “anywhere” using mobile phones and Bluetooth or WiFi technologies. However, in the veterinary profession, connectivity is an issue as high quality network service is lacking in most remote areas. Some other terms frequently interchanged with e-learning are online learning, online education, distance education, distance learning, technology-based training, web-based training and even computer-based training (generally thought of as learning from a CD-ROM). e-learning is a broad term used to describe learning done at a computer.

9.0. Introduction

Enzymes are organic biocatalysts which initiate and control biological reactions. A catalyst is an agent affecting the velocity of a chemical reaction without appearing among the final products of the reaction. Unlike general catalysts, enzymes are specific in nature and function at a temperature of approximately 37^o C and pH close to neutrality. All enzymes are proteins but all proteins are not enzymes. These are distributed among the cell and tissues of plants and animals. Use of malted barley in brewing, yeast in the production of alcoholic beverages and wrapping of meat in the bruised leaves of papaya tree for tenderizing meat are techniques that have been used for centuries. In bread and cheese making also, enzymes have been exploited from generation to generation. Thus, enzymes have been used without knowing the nature of enzyme as catalysts and reactions involved. However, it is only recently that nature and action of enzyme is known and its importance in food industry is realized.

Aim

To measure horizontal distance on the level ground using chain

Materials required

Chain

Ranging rods

Chain pins

Field note book

Bone healing can be modified by variety of endogenous or exogenous factors that affect the metabolic function of bone cells. The Food and Drug Administration is approved the use of low-intensity ultrasound for the accelerated healing of fresh fractures in October 1994 and for the treatment of established nonunion in February 2002. The first regulatory approval is based primarily upon two rigorous, double - blind, placebo controlled clinical trials, which shows that the rate of healing of fresh fracture is accelerated by treatment with ultrasound. Therapeutic ultrasound has been able to improve functional activity, reduce post-ambulatory period, alleviate suffering and restore the animal’s health early. It has been used frequently for acceleration of healing in various musculotendinous injuries in animal. However, the role of ultrasound therapy in different dose levels in fracture healing has not been elucidated properly. In view of the above fact, this chapter is undertaken to investigate the effects of therapeutic ultrasound in two different doses, on cortical bone healing in rabbits.

Twelve clinically healthy adult New Zealand white rabbits of either sex are divided equally into three groups A, B and C, and kept on standard diet and uniform management. In all animals, mid-shaft simple transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia. All the animals are administered broad-spectrum antibiotic and analgesic and surgical wounds are dressed daily with povidone iodine and antibiotic cream for 4 days postoperatively. Group A animal is received pulsed ultrasound therapy @ lW/ cm² (1:1) whereas, group C animals is received continuous ultrasound therapy @ 3 W/cm², starting on day 4 after the creation of fracture. Ten treatments, each consisting of 5 min duration, are given on alternate days. Group B animals are served as control. In both the test groups (A and C), the transducer head of the ultrasound machine is moved in a linear fashion along the length of the bone after applying ultrasound gel.

Carbon is the cornerstone of all life on Earth. Plant tissues and microbial cells contain approximately 40 to 50% of carbon on dry weight basis. Yet, the ultimate source is the CO₂ of the atmosphere. The CO₂ is converted into organic carbon by the action of photoautotrophic organisms (higher plants and green algae). It has been estimated that the vegetation on earth’s surface consume some 1.3 x 10¹⁴ kg of CO₂ per annum which is about V₂₀ of the total CO₂ supply of the atmosphere or ¹/₁₀₀₀ of that in the ocean.

Various indicators like range of behavioural, physiological, pathological and carcass-quality parameters can be used to assess the welfare of animals. Whenever, animals undergo painful handling or any other managerial operation, they are likely to face short-term stress. In this condition an increased physiological responses, behavioural responses, injury or mortality are most commonly used; however, some animals which are kept in improper housing with less floor space may cause chronic stress leading to increased disease incidence and suppression of normal development. Therefore, the following techniques can be used to assess the animal welfare at farm level in any given situation.

Processing

On arrival in the laboratory, the specimens are processed immediately or refrigerated until processed. For inoculation into animal/cell culture/embryonated eggs (EE), swabs are shaken in fluid medium, faeces are dispersed in fluid medium and tissue specimens are homogenized in a high speed blender/pestle and mortar with the help of an abrasive like powdered glass or sand. Good quality hard sand should be properly washed in distilled water, treated with HCl, washed thoroughly, dried and sterilized before use. Generally the infective tissue is homogenized in a few ml of buffer usually having a pH of 7.2 to 7.3 to make about 10% tissue suspension. The suspension is centrifuged at about 1000g for 10-15 minutes to sediment the tissue debris and coarse materials.

Abstract

Gastrointestinal parasitic infections particularly Haemonchus contortus infection in goats is very common and found as major nematode infections in farm condition. To chalk out the control programme and to prevent the initiation of anthelmintic resistant parasites, it is essential to know well in advance the prevalence of H. contortus infection. During the year 2009, a study was undertaken on H. contortus infection in the goat farm of ICAR Research Complex for NEH Region, Umiam, Meghalaya, which is a subtropical hill area and bioclimatograph was prepared to predict the prevalence of H. contortus infection in goats of this area. Meteorological data of this area was collected to visualize the effect of temperature, rainfall and humidity. Bioclimatographs were prepared in which total rainfall (TRF) or relative humidity (RH) is plotted against the maximum temperature (T-max) or minimum temperature (T-min) for the months and the resultant points are joined by a closed curve. The lines constituted the windows on these bioclimatographs, indicating the limit of the climatic conditions most suitable for development and survival of exogenous stages of gastrointestinal nematodes.