eChapters

ABSTRACT
The global energy demand is increasing day by day. Now-a-days, the supply of global energy is highly dependent on fossil fuels (viz., crude oil, lignite, hard coal, natural gas). But, on one hand the source of fossil fuel is decreasing at a rapid rate, and on the other hand the burning of fossil fuel is also degrading the environment due to huge Greenhouse Gas (GHG) emission (FAO/CMS, 2011). Hence, there is an urgent need of an alternate clean energy source that can sustain the future development undeterred while still protecting the environment. Biomass is considered as a sustainable source of clean energy that is useful in steam, fuel and chemicals production. It can be digested in controlled environment to produce biogas. The biogas is a clean, non-polluting and smoke-free fuel (Weiland, 2010) which can be used for applications that include cooking, space cooling/refrigeration, heating, electricity generation and gaseous fuel (CNG) for vehicles (Weiland, 2003). This paper analyses possible alternatives to process and utilise biogas for energy at industrial scale as a petroleum fuels replacement.

ABSTRACT
The rapid industrialisation and speedy progress towards urbanisation across the world have created the huge demand for energy moreover creating enormous quantity of wastes. The latter is in charge for polluting the environment in an exponential manner, resulting out of the harmful and poisonous compounds released by them. In recent years, there has been a pattern change from ‘waste-to-wealth’, considering the value of high organic fraction accessible in the wastes. The best-accomplished methods are that of anaerobic digestion; contribute to the production of methane (CH₄). Similar biotransformation has partial net energy harvests. Recently, fermentation industries steering their interest towards the production of value added products, viz., biohydrogen (H₂), biopolymers, ethanol, vitamins, acetic acid, enzymes, butanediols, etc. from carbon rich organic waste like cellulosic substrates have been envisioned to flourish in a multi-step process, such as the ‘Biorefinery’. A contemporary development in fermentation technology holds the key to overcome the constraints for such processes that may lead to a sustainable economy. One related technology is electrofermentation that has attracted significant attention recently owing to its capacity to boost the metabolic activities of microbes by means of extracellular electron transfer.

ABSTRACT
Biomethanation is the process of generating energy from anaerobic digestion (AD) of organic refuse. This helps reduce dependency on fossil fuels while reducing CO₂ emission. Organically-rich kitchen refuse (KR) generated daily from the households all over the world could be a good candidate to biogasify, though the quantity and quality of biogas depends on the KR proximate. To enhance biogasification, KR is subjected to physical, chemical and/or biological pretreatment. Physical pretreatments include chopping, grinding, ultrasonication and steam explosion, whereas chemical pretreatments could be by an alkali or a base. Biological treatment involves digestion of the organic matters by individual microbe or its consortium, although it takes place naturally. For biological treatment, standardising the substrate and inoculum ratio is essential. Candidate strains must be mutually compatible to formulate a microbial consortium. Bacillius seems to be a potential candidate as it is a facultative anaerobe exhibiting multiple essential enzyme activities (viz., amylase, cellulase and lipase). Maintaining an optimal pH of 7.0 and temperature of 37°C before and during anaerobic digestion is suggested. Other important factors in digestion include organic loading rate, retention time, C/N ratio, volatile fatty acids (VFA) concentration, etc. VFA content is vital as its accumulation during acidogenesis adversely affects the AD by dropping pH values to unfavourable levels. Acetogens convert VFA to acetate, CO₂ and H₂ in acetogenesis, finally converting it to CH4 in methanogenesis. For effective methanation,

ABSTRACT
The annual accumulating organic wastes generated through milling, brewing and various other agro-industrial and agri-processing activities in India amount to about 500 MMT. Most of these contain three major structural biopolymers, viz., cellulose, hemicellulose and lignin, a large proportion of which is carbohydrate and phenolic in nature. Massive accumulations of this kind of recalcitrant biomass not only deteriorate the environment, but also generate huge amount of potentially useful materials. Biological transformation has globally become increasingly popular among researchers and academicians in treating agricultural, industrial, organic and toxic wastes these days. About 60% by weight of the original pineapple fruit in forms of peeled skin, core, crown end, etc. resulting from the pineapple processing remains unutilised being discharged as waste, causing disposal and pollution owes. Of the 10% dry matter content of such waste, about 96% is organic and 4% is inorganic. Being rich in cellulose and hemicellulose, it can be a valuable resource for numerous value-added products by bioconverting it to valuable products, including bioenergy. Biomethane is one such energy resource that can be produced by valorizing pineapple wastes by employing various bioconversion technologies. This chapter focuses on the bioconverstion technologies to produce biomethane from pineapple wastes.

ABSTRACT
There is a quantum global growth in both number and size of dairy industries in recent times. These industries discharge thousands of liters of wastewater characterised by a heavy chemical oxygen demand (COD), biological oxygen demand (BOD), nutrients, inorganic salts, besides detergents and sanitisers each day (Kushwaha et al., 2011). High carbohydrates, fats and proteins contents in such waste streams originating from milk pose challenge to conventional municipal sewage treatment systems (Demirel et al., 2005). The easily biodegradable dairy effluent leads to precipitation of casein which is toxic and has a strong odour. Without adequate treatment, these effluents could pose serious threats to the receiving water bodies and disturb the ecosystem (Satpathy et al., 2017). For such heavily loaded organic effluents, biogasification through anaerobic digestion seems a promising strategy. This cost-effective energy generation process is also effective in environmental management compared to its other eco-friendly renewable source counterparts, such as, hydro, solar and wind (Satpathy, 2017). The Upflow Anaerobic Sludge Blanket (UASB) reactors are the most popular anaerobic systems to treat the fluctuating organic loads in dairy effluents. Anaerobic filter (AF), Upflow Anaerobic Filter (UPAF), Fixed-bed anaerobic reactor, Anaerobic Hybrid Reactor (AHR) etc. are few other systems that are demonstrably competent removing COD upto 90% and generating biogas. Successful anaerobic systems reported yielded (with upto about 85% CH₄ content) 0.32-0.34m3 CH₄/kg COD removed. Biomethanation process and reactors designs are constantly being optimised for effective treatment of the effluents while maximising the biogas production. The energy-rich gas thus obtained could further be utilised for heat and electricity at the domestic level, and also as a vehicular fuel. Residual digestate can also be used as organic manures (Angelidaki et al., 2007).

ABSTRACT
Rice straw is one of the most abundant lignocellulosic agricultural wastes in India and can be used as a major resource for biogas production. The structure of rice straw is complex and is made up mainly of cellulose, hemicelluloses and lignin. The lignin physically shields the cellulose and hemicelluloses, the main components which can be converted to energy, and hinders the access of microbial enzymes to these carbohydrate moieties. To overcome this making the straw a potential resource of biogas production, effective pretreatment is necessary that can efficiently hydrolyse the straw and make it accessible to cellulolytic enzymes and methanogens. Various pretreatment methods which are employed currently in the market are discussed here. For a process to be economically feasible it is necessary that the pretreatment be efficient as well as cost effective. To gain some insights into this aspect, the techno-commercial feasibility of Biomethanation of rice straw has been discussed.

ABSTRACT
Recent challenges for fossil fuels, associated environmental issues with disposal of organic garbage, and the rising costs for energy has encouraged researchers to search for alternate energy sources. Massive uncontrolled biogas from landfills, sludge and organic material degradation under anoxic conditions is an environmental management issue. Methane emission to atmosphere has a tremendous green house effect, but this can also be a valuable renewable energy resource. Biogas from kitchen wastes through anaerobic digestion by methanogens is such an alternate technology. Kitchen wastes are considered one of the best raw materials for biogasification. Biogas is mainly composed of 50-74% methane (CH₄), 25-28% carbon-di-oxide (CO₂), other volatile compounds including hydrogen sulphide (H₂S), water vapour and other trace gas compounds. The impurities in it, like Hydrogen sulphide (H₂S) which is corrosive, downgrade its quality. Purified biogas of higher calorific value attracts attention as a substitute fuel for additional applications. Its purification using various suitable scalable and economically-viable scrubbing technologies to enhance its quality and performance as an alternate fuel is an important step in this direction. Thus, a technology to generate and purify biogas from kitchen waste has significant prospects in the energy sector, and also as a promising enterprise to employ the youth.

ABSTRACT
Increased environmental pollution and high demand over supply for conventional fossil fuel sources have led to the exploration of other renewable energy sources. Out of the potential alternatives, methane has come up with a promising unconventional fuel source. Several technologies have been developed to establish methanation, including biomethanation, chemical methanation and gas-to-liquid technology. Several factors like process parameters, catalysts and reactors have been studied for process optimisation and making methane production commercially viable. Although methane as a greenhouse gas is a contributor to global warming, its combustion produces relatively ‘environment friendly’ gas CO₂, and H₂O. The released CO₂ is fixed into biomass by plant through photosynthesis process. Only rarely does methane combustion produces CO when combusted in an oxygen-limited condition. Thus, methane production and combustion are carbon neutral process. This chapter focuses on the application of methane as a source of alternate energy. Various technologies used, recent advances and challenges faced in methanation process are discussed.

ABSTRACT
Biomethanation comprises of four interdependent stages in series, hydrolysis, acidogenesis, acetogenesis and methanogenesis. Methanogenesis, the final stage of biomethanation, is accompanied with the reduction of substrates that include organic acids such as CH₃COOH, and others such as CO₂ and CH₃OH to CH₄ by methanogenic and methanotrophic archaea, usually under anaerobic conditions. Strictly anaerobic methanogenic archaea are found in the ruminant intestine, sewage digester, groundwater and deep soil/water etc. In methanogenesis, H₂ acts as the electron donor while substrates CO₂, CH₃OH and probably CoM-S-S-CoB (for CH₃COOH) act as terminal electron acceptors and reduce to methane, a process known as anaerobic respiration. This conversion is associated with the electrogenic proton motive force along with sodium motive force to drive the ATP synthesis by A0A1 ATP synthase, known as respiratory phosphorylation. The mechanism of conversion of methane from CO₂ by methanogens with cytochromes is same as those without cytochromes except that the reduction of heterodisulphide CoM-S-S-CoB is performed by the membrane-bound VhoACG-HdrDE complex for former while by cytoplasmic MvhADG–HdrABC complex for the later.

ABSTRACT
Methanogenesis is an important biogeochemical process takes place in anaerobic ecological sites. It is the last step of terminal electron accepting process of anaerobic respiration. Methanogens synthesise CH₄ using acetate and CO₂ as precursor molecules. Former types of methanogens are referred as acetoclastic methanogens and the later ones as hydrogenotrophic methanogens. After biogenesis of CH₄, it is emitted to atmosphere and causes global warming. In aerobic ecosystem CH₄ is oxidised by methanotrophs to CO₂ a less global warming molecule. However, in anoxic environment CH4 acts as an electron donor and interacts with various electron acceptors. Methane modulates denitrification, Fe reduction, SO₄ reduction and organic matter decomposition. Manuscript elucidates methanogenesis and highlights the pathways of interaction between methanogenesis and cycling of electron acceptors in various anaerobic ecosystems in relation to global C and N balance.

ABSTRACT
Methanogens are primarily anaerobic Archaebacteria representing the most primitive earthly creatures, alive since about 3.5 billion years. While oxygen inhibit their activity, they adapt well in a variety of ecological niche, viz., the intestinal tracts of insects and animals including ruminants, sewage digesters, groundwater and deep soil/water, etc. They are involved in biomethanation that converts organic polymers, including the complex recalcitrant lignocelluloses, to CH₄ and CO₂. Enhancing the process by optimising the physical, chemical and molecular parameters is an interesting biotechnology. Molecular research is rapidly progressing on the genomics, gene and its regulation, and methanogenesis as it expresses. Identifying novel species expressing methanogenesis and manipulating the genetic make-up including through cloning in recent years will set goals and leads for future investigations. The biomethanation enzyme Methyl-CoM-reductase (Mcr) constitutes about 10% of the methanogen protein. Due to its abundance and significance, elucidating its structure and its synthesis and regulation mechanism has received much attention recently. Mcr-coding genes from methanogens have been cloned and sequenced. An approach at the molecular level to enhance biomethanation seems to lie in metagenomics. The microbiology, biochemistry, the roles of the participating microbes, their molecular biology and applications in methanogenesis are discussed in this chapter.

ABSTRACT
Methanogenesis is a ubiquitous process primarily encountered in anaerobic environments. Methanogens are a group of diverse obligate anaerobic bacteria that produce methane from complex organic matter. Despite their differences in morphological characters and habitat, they show coherence in terms of methane-producing metabolic activity. Being distinctive from all other classical prokaryotes, these are grouped under another kingdom of Archea. Other distinctions of the members are based on their unique cell wall structures and membrane components, as well as differences in their highly conserved gene sequences. Methanogens likely owe their cosmopolitan status to their unique mode of energy metabolism of methane generation. They are active in various ecological niches, from rumens of animals to submarine volcanic vents, sewage digestors and landfills. Methanogenic bacteria are abundant in habitats where high affinity electron acceptors are negligible. Methane fermentation involves bacteria that obtain energy by catalysing anaerobically degradable organic matter to CH₄ and CO₂ by consuming H₂, CO₂, and low-carbon organic acids as their substrates. The gaseous products of methanogenesis are greenhouse gases, thus are major contributors to global warming. Methanogens could be used to produce renewable carbon-neutral energy substitute, biogas. Methane cycling archaea play a major role in global methane production as energy for the future.

A. General
        
B. Turf Grass
        
C. Indoor Plants

A. General
        
B. Bulbous Plants
        
C. Greenhouse Technology
        
D. Plant Breeding
        
E. Plant Physiology
        
F. Plant Tissue Culture and Biotechnology
        
G. Post Harvest Technology

Accent : It is created in the garden to avoid the monotonous view. Mostly unusual objects like tall fountain, tree, statue etc are used to create the effect.

Acre: A unit of measurement of land area equivalent to 4000 sq.met or 0.4047 hectare.

Acrylic: It is a glassy thermoplastic that is incorporated in rigid plastic to enhance wither ability.

Aging: Refers to the processes acquiring maturity with the passage of time or normally an increment of time, which may or may not be accompanied by physiological changes including senescence. Aging thus includes a much ‘wider span’ of physiological processes, which may either “weaken the organism or to be neutral”.

Agmark: It is Agriculture Produce, Grading & Marketing Act, 1937 & voluntary in nature.

Anaerobic respiration: An incomplete oxidation of food to release partial energy takes place in the absence of atmospheric oxygen and occurs in cytoplasm.

Analytical chemistry: Deals with detection and estimation of elements and compounds.

A. General
        
B. Leaves and Floral Terminologies
        
C. Diversity in the Flower
        
D. Diversity in Fruits
        
E. Terminologies Related to Seed

• Definition of Bioinformatics
• History of Bioinformatics 
• Structural Bioinformatics
• New Branch of Bioinformatics 
• Bioinformatics - Application in Agriculture
• Analyzing Protein Sequence
• Pairwise Alignment
• Multiple Alignment
• Blast - A Sequence Alignment Tool
• EMBL
• SWISS–PROT
• Rice Genome Project
• Software for Bio-informatics
• Major Categories of Bioinformatics Software
• Internet Sites For Bioinformatics

• Web Portal
• Types of Portals

• Agriculture Database Concepts and Importance
• The Orientation of Topic Selection for the Construction of the Agricultural Database
• The Principle of Specialization in the Construction of the Agricultural Database
• Top Quality Principle of Building Databases
• Agriculture Database Types
• Agriculture Libraries
• Agriculture Journals
• Technical Reports
• Conference Papers
• E-Books in Agriculture

• Database
• Data Model
• Database Software
• DBMS Facility 
• Architecture for a DBMS
• Data Modeling
• Components of a Data Mode
• RDBMS (Relational Database Management System)
• Client/Server Model of Computing
• Entity Relationship Diagram (E-R diagram)
• Entity Relationship Diagram Notations - Front-end and Back-end
• Codd’s Rules
• SQL - Structured Query Language
• Data Definition Language (DDL)
• Data Manipulation Language (DML)
• DCL
• Data Control Language
• Transaction Control (TCL)