eChapters

1. Introduction

Medicinal plants continue to be an important therapeutic aid for alleviating the ailments of humankind. The search for eternal health and longevity for remedies to relive pain and discomfort drove early man to explore his immediate natural surroundings to the use of many plants, animal products and minerals etc for the development of a variety of therapeutic agents. Today, there is a renewed interest in traditional medicine and an increasing demand for more drugs from plants sources. This revival of interest in plant derived drugs is mainly due to the current widespread belief that “green medicine” is safe and more dependable than the costly synthetic drugs, many of which have adverse side effects.

Vitex negundo or Five-Leaved Chaste Tree is commonly known as Nirgundi in India. It is a large aromatic shrub belonging to the Verbenaceae family of plants. It is distributed throughout the greater part of India up to an altitude of 1500 m in the outer Himalayas. It is a gregarious shrub that is found abundant along the banks or rivers, in moist situations, open waste lands and near the deciduous forests. It is widely planted as a hedge plant along the roads and between the roads.

13 1. Rishi Krishi

Drawn from Vedas, the Rishi Krishi method of natural farming has been mastered by farmers of Maharashtra and Madhya Pradesh. In this method, all on-farm sources of nutrients including composts, cattle dung manure, green leaf manure and crop biomass for mulching are exploited to their best potential with continuous soil enrichment through the use of Rishi Krishi formulation known as “Amritpani” and virgin soil. 15 kg of virgin rhizosperic soil collected from beneath of Banyan tree (Ficus bengalensis) is spread over one acre and the soil is enriched with 200 lit Amritpani.

It is prepared by mixing 250 g ghee into 10 kg of cow dung followed by 500 g honey and diluted with 200 lit of water. This formulation is utilized for seed treatment (beej sanskar), enrichment of soil (bhumi sanskar) and foliar spray on plants (padap sanskar). For soil treatment it needs to be applied through irrigation water as fertigation. The system has been demonstrated on a wide range of crops i.e. fruits, vegetables, cereals, pulses, oilseeds, sugarcane and cotton.

Important Commercial Mango Varieties

Northern Region: Langra, Dashehari, Chausa, Bombay Green, Amrapali, Sukul, Sipia

Eastern Region: Himsagar, Langra, (syn. Malda), Krishna-Bhog, Zardalu, Gulabkhas, Fazli.

Southern Region: Banganapalli, Bangalora, Mulgoa, Neelum, Swarnarekha,

Western Region: Alphonso, Kesar, Pairi.

Losses resulting from pre harvest and harvest drop of fruits have long been a serious problem to the fruit growers. As the fruits of some species and varieties approach the picking maturity, they tend to loosen from the stalk and considerable quantities may drop prior to and during the picking operation. Such fruits are badly damaged and if salvaged have a very low value compared with those picked from the tree. In certain fruit crops, the problem of fruit drop is very serious. Whereas in some, the fruit drop starts right from the time of fruit set and is severe at a number of stages of fruit development. In some fruit crop serious drop occurs before harvest.

Introduction

The rubber tree is the most important commercial source of natural rubber, an industrial raw material of strategic importance, recovered from its latex. Hevea was first used as a source of rubber by the French. The name rubber is derived from the quality of material in rubbing pencial marks. It was introduced to tropical Asia in 1876 through Kew Garden in the UK with seeds brought from Brazil by Sir Henry Wickham. It is now distributed in the tropical regions of Asia, Africa and America. The major rubber-growing countries are Thailand, Indonesia, Vietnam, Cote d’Ivoire, China and India Thailand has the maximum area under rubber, but China has taken the credit for maximum rubber production. India is the second largest producer and consumer of natural rubber in the world. In India, rubber is grown over an area of 7.57 lakh hectares with a production of 12.57 lakh tonnes, 9% of the total world natural rubber output. In India, Kerala is the predominant rubber-growing state. International Rubber Research and Development Board (IRRDB), Kuala Lumpur, Malaysia.

• Association of Natural Rubber Producing Countries (ANRPC), Kuala Lumpur, Malaysia
• Rubber Research Institute of India (RRII-1955) is located at Kottayam, Kerala

Moments

In statistics, moments are measures of the shape and variability of a data set. They are used to describe the location and dispersion of the data. There are several types of moments that can be calculated, each providing different information about the data set.

Moments is a term which refers to a measure of force with respect to its tendency which provides rotation. The strength of the tendency depends upon the amount of force and distance from the point of origin where force is exerted.

So total moments of all the forces about origin, if total moment is divided by the total force, which is called moment actually arithmetic mean for frequency distribution.

Hence, arithmetic mean is called first moment about the origin

• Arithmetic mean of various powers expressed as deviation from mean in any distribution is called the moment of the distribution.

Objective

After performing this experiment you will be able to learn:

Special quality assurance needs, good manufacturing practices, HACCP, validation protocols, etc.

Introduction

Packaging is a very important element in product quality maintenance that is why quality of packaging has great influence and its quality assurance is necessary. Physical flow in the supply chain may deteriorate some packaging features, so their monitoring and proper conditions of storage, transport and suitable protection are important.

The quality of packaging is essential; it is an important element of product quality and supply chain effectiveness. Packaging is influenced by quality of raw materials, proper conduct of the production, storage, transport and distribution processes in a company. That is why a lot of companies implement quality assurance or management systems. In regards to packaging risk categories there are different requirements.

Emerging Infectious Diseases
Emerging infectious diseases are those due to newly identified and previously unknown infections which cause public health problems either locally or internationally.
Re-emerging Infectious Diseases
Re-emerging infectious diseases are those due to the reappearance and increase of infections which are known, but had formerly fallen to levels so low that they were no longer considered a public health problem.
What Causes Emergence or Re-emergence of Infectious Diseases?
Several factors contribute to the emergence and re-emergence of infectious diseases, but most can be linked with the increasing number of people living and moving on earth: rapid and intense international travel; overcrowding in cities with poor sanitation; changes in handling and processing of large quantities of food; and increased exposure of humans to disease vectors and reservoirs in nature. Other factors include a deteriorating public health infrastructure which is unable to cope with population demands, and the emergence of resistance to antibiotics linked to their increased misuse.
Avian Influenza
Avian influenza is a zoonotic, globally important disease of birds that can be categorised as either low pathogenic (LPAI) or highly pathogenic (HPAI) according to the virulence of the virus in animals. Outbreaks of LPAI are common around the world but LPAI typically causes no clinical signs or only minor illness in infected birds. LPAI strains are generally less of a threat to human health; in addition, LPAI can have significant economic repercussions as a result of export restrictions and culling of birds, particularly in developing countries where the use of vaccines and other veterinary medicines is difficult
due to weak veterinarian services and small farming units. Compared with LPAI, HPAI is more readily detected in poultry due to very high levels of mortality in infected birds, and can cause potentially catastrophic economic consequences, from significantly reduced livestock populations to lost export markets. Though relatively rare, sporadic human infections of HPAI have occurred in cases of close contact with infected birds and caused serious illness
and even death.

A watershed is an area that provides pathway to the surface or sub surface water flow to a given drainage system. The watershed is a hydrological unit. The area of watershed may vary from few hectares to thousands of square kilometres. The watershed development involves effective management of land and vegetation in order to conserve and improve the quantity of water. The watershed development is a comprehensive and sustainable solution to water conservation (Fig.13.1).
The watershed development has several advantages. It helps in
• Conservation, upgradation and utilization of natural resources particularly land, water and vegetation
• Minimise the inequalities between rainfed and irrigated areas
• Promoting cost effective adoptable simple technologies
• Supply water for domestic ,agricultural and industrial use
• Poverty alleviation and employment generation
• Reducing chemical pollution
• Adopting flood control measures by constructing water storage or impounding structures

Principle

The infectivity of a virus may be neutralized by specific antibody when they are mixed and incubated. In some cases, the addition of homologous or guinea pig complement to the mixture enhances neutralization, particularly when early antiserum is used. Serum must be inactivated by heating at 560C for 30 minutes to inactivate complement or to remove non-specific virus inhibitors. Serum-virus mixtures are inoculated into appropriate cell cultures, which are then incubated until the only virus controls develop cytopathic effects. Antibody by neutralizing the infectivity of the virus, protects the cells against virus destruction.

1. Introduction

Food poisoning is a major concern for both the food industry and consumers inspite of different preservation practices some species of pathogenic bacteria causes food spoilage leading to outbreak of illness. Spices and herbs have been added to food since ancient times, not only as flavouring agents, but also as food medicine and as food preservative (Nakatani, 1994). Additives need for food preservation may be harmful to human health and has led to use of natural products especially plant extracts, their essential in food preservation.

More than 90 percent of the cases of food poisoning each year are caused by Staphylococcus aureus, Salmonella typhi, Vibrio cholerea, Proteus mirabilis, Pseudomonas aeruginosa, Clostridium perfringens, Campylobacter, Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus and Entero-pathogenic Escherichia coli. These bacteria are commonly found on many raw foods and cause severe disorder in humans. Spice posses anti adhesive properties that prevent the microbe to the host tissue, thus preventing the primary infection. It also possesses a rich array of phytochemical and a rich source of antioxidant. Some studies claim that the phenolic compounds present in spices and herbs might also play a major role in their antimicrobial effects.

Objective: To amplify DNA using RAPD primers and study on agarose gel.

Background information:

RAPD markers are decamer (10 nucleotide length) DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals. RAPD is applied to analyze the genetic diversity by random primers. Unlike traditional PCR analysis, RAPD does not require any prior DNA sequence information of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions complementary with primers' sequence. For example, no fragment is produced if primers annealed too far apart or 3' ends of the primers are not facing each other. Therefore, if a mutation has occurred in the templ that was earlier complementary with primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. RAPD is a dominant marker means it cannot distinguish between heterozygous; single copy and homozygous; two copies. Rarely RAPD markers may be Co-dominant where different fragment length of DNA is amplified from the single locus. PCR is a molecular technique used for amplification of DNA, a complete enzyme based chemical reaction, therefore, the concentration and quality of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible. Mismatches between the primer and the template may result in the total absence of PCR product or decreased amplified product. Thus, the RAPD results can be difficult to interpret.

COMPOSITION OF DIET

Objectives

• To study the general considerations on feeding aspects of laboratory animals.
• To get acquainted with the specific nutritional requirements of laboratory manures.
• To familiarize with the watering system.
• To know the daily feed schedule of different species of laboratory animal.

Materials Required

Laboratory animals, feed ingredients, water, green vegetables.

For having good growth and production, proper balanced diet should be fed, thus nutrients can be classified into 6 classes:

A washing machine laundry machine, clothes washer, or washer) is a device used to wash laundry. The term is mostly applied to machines that use water as opposed to dry cleaning (which uses alternative cleaning fluids, and is performed by specialist businesses) or ultrasonic cleaners. The user adds laundry detergent which is sold in liquid or powder form to the wash water.

Soil organic matter is among the most complex materials existing in nature. In addition to the organic constituents present in undecayed plant and animal tissues, soil organic matter contains living and dead microbial cells, microbially synthesized compounds and an endless array of derivatives of these materials produced as the result of microbial activity. Soil organic matter probably contains most of the naturally occurring organic compounds. Some components of soil organic matter are no doubt distinctive to the soil environment, particularly those involving inorganic-organic complexes.

6.1. Composition of Soil Organic Matter

The composition of soil organic fraction is as follows:

1. Components of organic residues undergoing decomposition

Wet litter can be caused by extrinsic or intrinsic factors related to excessive excretion of water. When the moisture content of litter exceeds 35%, it can lead to various issues, including pododermatitis of the foot pads, resulting in poor growth rate in broilers and low fertility in breeder flocks. Wet litter can also contribute to inflammation of the feather follicles. This can lead to gangrenous dermatitis, which is responsible for downgrading at processing or rejection by consumers. Additionally, wet litter is often associated with outbreaks of coccidiosis, as the oocysts of Eimeria spp. require moisture levels over 25% to mature. Necrotic enteritis occurs frequently in houses with areas of wet litter, while damp litter may contribute to the proliferation of toxic fungi.

22.1 Introduction

The blood vascular system of fishes is modified to suit the aquatic mode of life. Fish contains the simplest single-circuit closed blood vascular system.

The structure of the heart in fish varies little. In fish, all of the chambers of the heart are essentially the same. The size and shape of the component chambers may differ. A large heart is found in extremely sluggish fishes with low oxygen requirements. The number and arrangement of valves in the conus arteriosus are the most distinguishing features in various fishes.

· Plants have structural and biochemical defense mechanisms against pathogens.

· Structural defense mechanisms can be pre-existing (e.g. wax, thick cuticle) or post-infectional (e.g. cork layer formation, tyloses).

· Pre-existing biochemical defenses include inhibitors and phenolics; post infectional defenses include phytoalexins and plantibodies.

· Disease development depends on a successful host-pathogen interaction; defense mechanisms are ineffective during a compatible reaction.

Hydrometeorology forecasting is limited to forecasting of occurrence of rainfall to a greater extent. Although several countries all over the world have developed and established hydrometorological forecasting with focus on occurrence of floods and droughts, still there is very limited success. Floods are associated with occurrence of continuous heavy rainfall due to movement of low-pressure systems like cyclones and depression overflow into rivers, reservoirs and breaching of bunds. In countries like India, the floods occur on the average in 70 lakh hectors of area and the economic loss due to floods and expenditure on relief operations mounting year after year besides loss of life. Foods occur at all of a sudden leaving little time for planning mitigation or rescue operation. Flood forecasting in based on impact assessment of heavy rainfall assessments to identify the exact area that is likely to be affected. It requires more intensive research. Forecasting of droughts is still an area of serious concern. There is need to strengthen regional capabilities to develop forecasting techniques for occurrence of flood and droughts through proper analysis of historical data in conjunction with meteorological forecasts and remote sensing data. Though flood alerts are issued, the identification of exact area and the extent to which flood impacts are not very clear most of the times heavy rainfall warnings are not just sufficient until and unless the vulnerable areas can be clearly specified. The medium range forecasting will be extremely useful for issuing flood alerts.
14.1. Forecasting Droughts
Long range forecasting of seasonal rainfall can indicate the possibility of receiving excess or deficit rainfall over larger regions. In India, there is considerable progress in development of long range forecast of Southwest monsoon rainfall. This forecast also indicating the regions which may get surplus or deficit rainfall and for indifferent parts of a given region as well. However, there is serious dearth of local expertise to further analyse and interpret the long-range forecast for a given area on the basis of local climate data. The advantages of reliable long-range forecast are still unexpected. Almost one fifth of the area in India gets affected by draught most of the years. The drought affects are more severe in arid and semi arid regions. Prediction of occurrence of drought and its intensity, period of occurrence and the areas to be affected by drought is very important. During recent years, there is considerable increase in the occurrence of droughts due to climate change which is attributed mainly to atmospheric pollution. The physics of the pollution is consistent and not the policies support in handling hydrometerological disasters. There are instances where in flood and droughts occurred in the same

Natural gas: Produced from gas wells or oil wells rich in natural gas. It is composed mainly of methane, but it also contains small amounts of ethane, propane, hydrogen, helium, carbon dioxide, nitrogen, hydrogen sulphate, and water vapour. It is stored either in the gas phase at pressures of 150 to 250 atm as CNG (compressed natural gas) or in the liquid phase at 162° C as LNG (liquefied natural gas).

Natural-draft cooling tower: Uses the naturally occurring density gradients between the inside air-water vapour mixture and the outside air which create an airflow from the bottom to the top of a wet cooling tower.

Needle valve: A valve in which the flow of liquid or gas is regulated by the adjustment of a pin or needlepoint, which sets in a cone-shaped depression having a small hole at the bottom.

Negative: One of the two terminals in an electrical circuit, presumably the one through which the current returns to the source.

Negative camber: The condition existing when two tyres are closer together at the top than at the bottom.