eChapters

1.1 Introduction

Pomegranate (Punica granatum L.) is a well-known table fruit of tropical and subtropical regions of the world. The Romans received it from Carthage, hence the name of the genus Punica. Some botanists place it in the family Lythraceae, of the peculiar type of fruit, called as balausta, most authorities make it the only genus in the family Punicaceae. It belongs to genera Punica and family Punicaceae (Chatterjee & Randhawa, 1952; Joshi, 1956). It is a small tree, measuring less than 4 m when cultivated, although it can reach 7 m in the wild. Recent research findings corroborate traditional use of pomegranate as a medical remedy as all parts of this plant have several bioactive metabolites (Holland et al. 2009). Consequently, its demand has increased tremendously not only in the western world but also in other parts of the globe.

1. Introduction

Medicinal plants are the most important source of life saving drugs for the majority of the World’s population. Plants have been an important source of medicine for thousands of years. Diabetes is one of the major degenerative diseases in the world today. It is a major risk factor for the development of cardiovascular disease. About 70 - 80% of deaths in diabetic patients are due to vascular disease. Diabetes mellitus is also associated with hyperlipidaemia with profound alteration in the concentration and composition of lipid (Odetola et al., 2006). Changes in the concentration of lipid with diabetes contribute to the development of vascular disease. Excessive levels of blood cholesterol accelerate atherogenesis and lowering high blood cholesterol reduces the incidence of CHD Grundy (1986). One of the risk factors for coronary heart disease is elevated Total Cholesterol (TC), Low Density Lipoprotein-Cholesterol (LDL-C) and Lower High Density Lipoprotein-Cholesterol (HDL-C) (Hu et al., 2001; Schaefer, 2002; Moreno and Mitjavalia, 2003; Jain et al., 2007). In particular, hyperglycemia, the primary clinical manifestation of diabetes, is thought to contribute to diabetic complications by altering vascular cellular metabolism, vascular matrix molecules and circulating lipoproteins (Inoguchi et al., 1994).

Objectives

• To keep the laboratory animal house neat and clean
• To maintain healthy stock

The disease may be bacterial, viral, fungal or of parasitic origin. All the measures taken to reduce growth and also to control spread of pathogenic organism is a sanitary measure like ventilation, building without cracks crevices.

Important aspect of good sanitation is insect control or pest control. Most effective measure is to just chase them out. To achieve this :

15.1. IFS Components Tested for Wetlands

Area Allocation;

In 1 ha of land

75 % - Cropping

10 % - Fodder cultivation

3 % - Goat shed

12 % - Fish pond

Possible Integrations:

The increasing awareness of the deleterious effects of indiscriminate use of artificial inputs in agriculture such as chemical fertilizers and pesticides hasled to the adoption of organic farming as an alternative method for conventional farming. Farmers are adopting organic farming due to the advantages like,

• It is self-sustaining and socially and ecologically superior over the conventional farming.
• It makes use of cost effective management practices involving the use of farm inputs produced within the farm.
• It is less expensive.
• It is environment friendly as there is no pollution of soil and water.
• It enriches the soil and the local ecology.
• The produce is free of contamination from chemical residues has better taste, flavour and nutritional value.
• Seeds have more vitality and are good for successive generations.
• Growing markets for safe food

11.1. Support for Start-Up Finance

Access to finance for start-ups is always difficult due to the problem of asymmetric information. Start-ups lack the kind of financial history which banks use to infer the pay back behaviour and the business capacities of the entrepreneur. Innovative start-ups face the additional problem that it is difficult to assess the viability of their business ideas or the prospects of the markets. In addition, banks and other potential investors often decline to get involved when the relatively small amount of capital requested by a new business is out of proportion with the efforts that the investors must make to screen and evaluate the start-up project.

These problems are even greater for companies engaging in IB models, as doing business with the poor is usually riskier - or perceived as more risky - due to BoP customers’ low ability to pay and the greater vulnerability of value chains. Support for start-up finance is therefore essential. Local IB start-ups often lack funding at all stages of the start-up process: the idea stage, when business opportunities are identified, the seed stage, when the market is assessed and the business model developed, and the start-up stage, when the company is formally set up and all the necessary equipment is procured. Different kinds of financial support will be necessary at all stages.

Management system adopted for fruit trees are quite different, especially with regard to nutrient than that adopted for field crops. The shift in management system could be commenced 3-4 years after planting depending on the tree size. Nutrients are required for flowering and fruiting while at the same time trees are allowed to grow and maintain sufficient vigour for producing high yields in following years. To maintain productivity of trees in long run and maintain the sustainability of tree production capacity, application of nutrient should be based on actual requirement and availability of nutrient in the soil. Application of plant nutrients economically at correct time with right amounts in a way that nutrients could be taken up by plants efficiently with minimum losses. The purpose of assessment of nutrient requirement of fruit trees is to keep mineral nutrient levels in the tree with in the desired range to have the growth and development effects and fruiting of trees as desired.

The most important thing in nutrient management for bearing trees is to analyze the importance of timing of nutrient application in relation to tree phenology or growth cycle.

Theory

Area computation is an important part of surveying. The term ‘Area’ is meant the projected area on a horizontal plane. The area is computed from the following methods: (i) Graphical method (ii) Instrumental method and (iii) Use of formulae. In graphical method, the area can be computed by dividing the whole area either into triangles or squares. The area of individual triangle is computed from base and the altitude and summation of individual area gives the total area. A tracing paper is divided into number of small squares is placed over the plan. The number of complete squares within the boundary is counted. The squares more than half are counted as full squares and those less than half are neglected. The total number of squares multiplied by the area of each square gives the total area of the field. In instrumental method, the planimeter is used to measure the area of a plotted plan. In use of formulae, computation of area by formulae depends on the shape of the field.

Introduction

As Rhizobia are slow growers so their enumeration by plating techniques is less recommended. Rhizobia can be enumerated in the presence of a large number of other organisms by most probable number (MPN) method. Soil containing Rhizobia is diluted in liquid and dilutions are used to inoculate roots of legumes. The presence of Rhizobia in a given experimental unit, inoculated by a given dilution, is indicated by the formation of at least one nodule on a root. Thus, the method involves the growth of legumes under conditions such that a single rhizobia cell can multiply and forms at least one nodule on the root.

Most Probable Number Method: This method permits estimation

Principal Scientist and I/c Head, Division of Social Sciences and Training IIHR, Hesaraghatta Lake Post, Bangalore – 560 089, Karnataka

Horticulture value chains are facing growing consumer expectations for variety, food safety and security. Most horticulture supply chains operate in a push-based approach rather than demand driven or pull based, which leads to a mismatch between demand expectations and supply side capabilities. In developed markets in, India, horticulture supply chains are experiencing by excess capacity, lack of differentiation and lower prices and ultimately leading to glut. 326 mt of Horticultural production for year ending 2019-20, the emerging market challenges are more related to supply shortage, lack of product variety, and safety and quality of the produce. 

23.1 Introduction

Excretion is the elimination of nitrogenous metabolic wastes from the gut and skin of the body. Through excretion, certain substances are removed while others are conserved. Fish are either ammonotelic or ureotelic animals. Kidneys play the most important role in excretion to maintain water-salt balance.

Fish gill ammonia excretion has been investigated for 80 years, but its mechanisms are still up for debate. A new paradigm has been established by the relatively recent identification of the ammonia-transporting role of the Rhesus (Rh) proteins, a family related to the Mep/Amt family of methyl ammonia and ammonia transporters in many living organisms. These proteins also occur as glycosylated proteins in fish gills (Wright and Wood, 2009).

A. Definitions of probability

1. A probability relies on having an understanding of the theoretical model defining the set of all possible outcomes of a trial; we evaluate the probability solely on the basis of this model, without recourse to performing the experiment at all. It is often called an a priori probability. So, for example, we know that there are two equally likely outcomes when an unbiased coin is tossed: either a head or a tail. This is the model from which we can deduce that the probability of a defined event, obtaining a head, say, is 1/2 = 0.5.

2. The next approach to defining a probability, and the one commonly used in statistical inference, is to regard a probability as the proportion of times a particular outcome (the event) will occur in a very large number of ‘trials’ or ‘experiments’ performed under similar conditions.

The result of any one trial should be independent of the result of any other trial, so whether or not the event occurs in any one trial should not affect whether or not the event occurs in any other trial.

There streams of thought are presented in this paper:

One, that rural women are not only the warriors, creative actors in the agricultural scene, or rural areas, but also the urban poor, the  migrants , the homeless, the source of labour supply into the expanding urban spaces, including trafficking.
 
I would then argue [two] that this spread of the location of “rural women” is the phenomena that we should now look at, the connectivity between the old divisions of rural and urban, as it indicates the pressures that the current development paradigms operating in our countries, is putting on the RURAL … and therefore that we should look at the macroeconomic policies that are in place, globally and regionally and  nationally.

Objective: To isolate RNA from plants using TRIzol reagent and its quantification by spectrophotometer.

Background information

RNA (Ribonucleic acid) is a polymeric substance present in living cells and many viruses, consisting of a long single-stranded chain of phosphate and ribose units with the nitrogen bases adenine, guanine, cytosine, and uracil, which are bonded to the ribose sugar. RNA is necessary in all the steps of protein synthesis in all living cells and carries the genetic information for many viruses.

The isolation of RNA with high quality is a crucial step required to perform various molecular biology experiment. TRIzol Reagent is a ready-to-use reagent used for RNA isolation from cells and tissues (Chomczynski, 1993).TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. Addition of chloroform, after the centrifugation, separates the solution into aqueous and organic phases. Aqueous phase contains RNA.

After transferring the aqueous phase, RNA can be recovered by precipitation with isopropyl alcohol. DNA and proteins can be recovered by sequential separation after the removal of aqueous phase. Ethanol precipitation needs DNA from the interphase, and an additional precipitation with isopropyl alcohol requires proteins from the organic phase. Total RNA extracted by TRIzol Reagent is free from the contamination of protein and DNA The isolated RNA can be further used in Northern blot analysis, in vitro translation, poly (A) selection, RNase protection assay and molecular cloning.

Paddy is cultivated about 45 m ha in India, less than 1% is under organic cultivation.

16.1. Varieties

Good quality fine varieties like Ponni, Super ponni, insect and disease resistant varities must be selected for getting higher yield and returns.

16.2. Seeds

Organically produced seeds only must be used.

16.3. Seed Treatment

For 1 kg of seeds 20 g Azospirillum, Pseudomonas and 10 g Phospobacteria must be used with rice gruel.

Panchagavya spray @ 1% for treating the seeds.

5.1 Graphical Representation of Data

Diagrams are generally useful for the purpose of publicity and propaganda. No statistical conclusion can be drowning as these provide only an approximate and rough expression about the phenomenon. Thus for a more rigorous and improved representation of numerical statements graphs and charts are used.

Graphs are generally drawn with the help of two perpendicular lines known as x-axis and y-axis. The following are the various types of graphs, which are in common use:

Aim

To identify different parts of prismatic compass and to know their functions.

To practice of taking the angular measurement.

Compass survey principle

Compass surveying is a branch of surveying in which directions of survey lines are determined with a compass and the lengths of the lines are measured with a chain or tape. In practice, the compass is generally used to run a traverse. In compass surveying, a traverse consists of a series of straight lines connected together to form an open or closed polygon as shown in Fig.44.1. The points A, B, C, D and E defining the ends of the traverse lines are called traverse station or traverse points. The lengths of the traverse lines are measured with a chain or tape. At each traverse station, where the traverse lines meet, the angle is measured with a compass.

11.1. Definition

Jones: Variations or modifications of any aspect of social process, social patterns, social interaction or social organization.

Gillin and Gillin: Variations from the accepted modes of life; whether due to alteration in geographical conditions, in cultural equipment, composition on the population or ideologies and whether brought about by diffusion or inventions within the group.

Davis and Mac Iver: Change in the relationships.

Among the epiphyseal and metaphyseal fractures of the different long bones, fractures of the proximal tibia are quite commonly reported and require special attention due to their proximity to the compound stifle joint. Hence, a standard fixation technique is needed to treat such fractures, which also permit early ambulation and preserve range of joint motion in addition to providing rigid fracture fixation. Different treatment modalities have been tried for fixation of metaphyseal fractures with variable results. No single method is appropriate in all circumstances and treatment indication often overlaps. Therefore, in this present chapter, an attempt has been made to evolve a standard technique for the management of such fractures.

Ten clinically healthy mongrel dogs are maintained under uniform housing, feeding and management conditions, acclimatized, immunized against rabies, dewormed and randomly divided into two groups (A and B) of five animals each. In all the animals unilateral metaphyseal fracture is created in the proximal tibia under general anaesthesia with intravenous thiopental sodium (5%). In the animals of group A, the fracture is immobilized with cross intramedullary Steinmann pinning with interfragmentary wiring, whereas, single intramedullary pinning along with interfragmentary wiring is performed in the animals of group B. Antiseptic dressing of the wounds is done daily for 1 week with povidone-iodine. All the animals are administered Enrofloxacin at the dose rate of 5 mg/ kg b.wt intramuscularly daily for 5 days posoperatively. Skin sutures are removed on day 10 post-operation