eChapters

2.1 Beakers

Beakers are the one of most important vessels used in laboratory which is made up of heat resistant glass.

They are available in various capacities ranging from 10 to 3000 mL.

For convenience of pouring solution, spout is provided in the beaker.

They are used to perform titrations & for boiling solutions, etc.,

Beakers are constructed in tall and low forms.

Ultrasound is an excellent diagnostic modality for detecting visceral abnormalities. Detection of masses, nodules or effusions through sonography provides non-specific diagnosis. For specific/definite diagnosis tissue samples are necessary for histopathological examination. The techniques of ultrasound guided fine needle aspiration and core biopsy are of great help in establishing etiological diagnosis.

Division of PHT & Agricultural Engineering, IIHR, Hessaraghatta Lake Pos Bangalore – 560 089, Karnataka

The supply chain management is the management of a network of interconnected businesses involved in the ultimate provision of product and service required by the end customer (Mentzer, 2007). The horticultural supply chain includes entire vertical chain of activities from the supply of input (seed, fertilizer, chemicals) through production, postharvest operations, distribution and retail. Presently, not many details are available in the public domain related to the supply chain management of agricultural commodities in India. There is a need for a new revolution to bring down the prices of agricultural produce for consumers through an efficient supply chain management and incentivize farmers to increase their production (Roy, 2015). 

Extensive studies have shown that much of the humified material in soil is finely bound to colloidal clay. However, it is uncertain to what proportion of the clay surface in any given soil is coated by organic substances as this will depend on the organic matter content and the type and amount of clay.

The ways in which organic substances are retained in soil are as follows:

1. As insoluble polymeric complexes of humic and fulvic acids.
2. As polymeric complexes of humic and fulvic acids bound together by di- and trivalent cations such as Ca²+, Fe³+ and Al³+.

Introduction

The study of fish health is crucial for understanding aquatic ecosystems and ensuring the well-being of aquatic species. This report provides a detailed account of a hands-on activity involving necropsy and health examination of fish. Through this practical experience, we aimed to gain insights into the common ailments affecting fish populations and develop skills in identifying pathological conditions.

The necropsy process involves the post-mortem examination of fish specimens, enabling us to observe and analyze internal organs, tissues, and external features. By combining practical techniques with theoretical knowledge, we seek to contribute to the broader understanding of fish health and disease.

Poultry hatchery is a place where the hatching egg is transformed into a one day old chick, during 21 days. The environment of a poultry hatchery is very susceptible to contamination by microorganisms which can adversely affect hatchability of the eggs and can result in embryonic deaths. E. coli, Staphylococcus species, Streptococcus species, Aspergillus fumigates and Pseudomonas species are mainly isolated from hatchery and it adversely affects chick quality and cause embryonic deaths. Poor standards of hatchery hygiene may lead ultimately to an explosion of pathogenic organisms resulting in severe economic loss.

India’s growing aquaculture industry requires the implementation of standardized laboratory techniques for detecting fish pathogens to uphold fish health, ensure biosecurity, and meet international trade standards. Both national and international diagnostic guidelines play a vital role in ensuring accurate identification of diseases, surveillance, and certification.
Domestically, the Indian Council of Agricultural Research (ICAR), particularly through institutes like ICAR-Central Institute of Fisheries Education (CIFE) and ICAR-National Bureau of Fish Genetic Resources (NBFGR), has developed diagnostic methods suited for Indian conditions. These include techniques such as histopathology, bacteriology, ELISA, PCR, and qPCR, specifically validated for key cultured species like Labeo rohita, Catla catla, Penaeus vannamei, and Clarias batrachus. The National Surveillance Programme for Aquatic Animal Diseases (NSPAAD) also outlines protocols for disease diagnosis and reporting, aligning with the standards set by the World Organisation for Animal Health (WOAH), previously known as OIE.
At the global level, WOAH offers comprehensive diagnostic manuals for key aquatic animal diseases like white spot disease (WSSV), infectious pancreatic necrosis (IPN), and epizootic ulcerative syndrome (EUS). These manuals detail procedures for collecting samples, using PCR primers, conducting serological tests, and identifying histopathological features. Adhering to these protocols is essential for maintaining the credibility of Indian exports to markets such as the EU, USA, and ASEAN countries. Additionally, laboratories with ISO/IEC 17025 accreditation ensure quality control and global recognition of their test results.

This chapter introduces the relationship between sheep breed, production system and the type and quality of wool produced. It classifies sheep into fine-wool, longwool, medium or crossbred, carpet-wool and hair breeds. Particular attention is given to Merino and Indian breeds such as Chokla, Magra, Marwari, Nali, Deccani, Gaddi, Rampur Bushair and Bhakarwal. The chapter also examines extensive, pastoral, mixed and intensive production systems and their influence on wool characteristics and economic value.

17.1. The Nigerian Unemployment Milieu: Control Measures taken by the Government

At this juncture, the little efforts that have been made by the government to control unemployment to an extent shall be examined. Records from the Central Bank of Nigeria (1987), show the following efforts made by the government:

1. Youths Empowerment/ employment and skills programme. This was done in terms of provided minor jobs or ‘white-colar’ jobs.

2. Agricultural programmes such as the ‘Operation Feed the Nation’ by the Obasanjo Military Administration.

3. Provision of small scale industries and graduates employment.

4. Special public works (which were temporary). Some of these efforts are still in place while others are forgotten. For example, on the pages of the Businessday Newspaper, on August 4th (pg 13), 12th (pg 49), 21st and 27th, 2009 were the following headings revealed:

Water used for irrigation contain some salts, but quantities and kinds vary greatly depending on the source of water. Since sodic status of soils can be greatly influenced by salts in irrigation waters, successful irrigation practices require the evaluation system to judge irrigation water quality and methods employed. The actual damage done by salt to plants or soils depends on the concentration in soil solution rather than the quality of irrigation water applied. Thus, the use of some water source for irrigation might lead to severe salt problem in soils where drainage is restricted.

Criteria for evaluation of irrigation water

Various criteria considered in evaluating the quality of irrigation water are:

1. Salinity hazard

The most satisfactory method for rating the salt content of irrigation water involves measuring electrical conductivity. Various conductivity limits have been proposed to indicate the degree of salinity problems that might be anticipated with use of irrigation water on crops. There are four classes of salinity hazard such as C₁, C₂, C₃, and C₄.

Introduction

Commercially laying of poultry birds carried out for a laying year or cycle after that as egg production decreases then they are usually culled when birds starts shedding of feathers. If we still keep these birds after allowing shedding and renewing of feathers birds take sufficient rest to builds up its body reserve of nutrients for next laying cycles. Their production regains 80-83% of the first laying cycle therefore recycling of laying can be carried out by shedding and renewing of feathers which is nothing but the “molting.”

Introduction
Antimicrobial resistance (AMR) is a critical public health problem globally, which can shake the foundation of modern healthcare. Infections caused by drugresistant organisms could lead to increased mortality and prolonged duration of hospitalization, causing a huge financial burden to the affected persons, healthcare systems and hinder the goals of sustainable development. With rising AMR, antibiotics are increasingly becoming ineffective for treating diseases.The post antibiotic era is not a dreadful imagination but a potential reality unless effective global actions are initiated now (1). AMR is not the only problem of human health, it widely affects the animal husbandry as well as environment. In India in 2019, there were 297,000 deaths attributable to AMR and 1,042,500 deaths associated with AMR. India has the 145th highest age-standardized mortality rate per 100,000 population associated with AMR across 204 countries.The number of AMR deaths in India is higher than deaths from neoplasms, respiratory infections and tuberculosis, enteric infections, diabetes and kidney diseases and maternal and neonatal disorders.Out of 10 million projected human deaths in 2050, one-fifth may be from India (2).Annually, more than 50 000 new-born are estimated to die from sepsis due to pathogens resistant to first-line antibiotics. The median cost of treatment of a resistant bacterial infection in India is estimated to be more than a year’s wages of a rural worker. There are five pathogens to be aware of in India (number of deaths associated with AMR in parenthesis): Escherichia coli (152,700), Klebsiella pneumoniae (123,200), Staphylococcus aureus (111,400), Acinetobacter baumannii (103,500), and Mycobacterium tuberculosis (98,600).If there is no timely containment, AMR is likely to cause nearly 10 million deaths by 2050 and resulting in significant economic losses. It would also impact nutrition security, livelihood and hinder the attainment of the Sustainable Development Goals. In 1928, penicillin was developed and socalled magic drug was subsequently commercialised and used globally. When antibiotic resistance ?rst emerged, novel antibiotics to treat these bacteria were developed. However, it is dif?cult to develop effective new antibiotics every time as it is time consuming and costly and if developed a new resistance mechanism emerges in bacteria. There is a demand for new strategies to treat bacterial infections, such as endophytes therapy, phage therapy, antimicrobial peptides, CRISPER cas and so on.

Analysis of materials or samples is essential to understand their nature and quality. In recent years it has become mandatory to display the composition of food as well as non-food items. This is because of the need for their safety from the point of view of their day to day use. Environmental awareness has further imposed many restrictions on items of wide ranging nature. Analytical information gained from a laboratory is highly useful in knowing the qualitative and quantitative composition of the material.

Clasping stemmed Solena or diversely leaved Melothria or creeping cucumber (Solena amplexicaulis Lam. Syn. Bryonia amplexicaulis (Lam), Melothria heterophylla (Lour), Melothria angulata Chakrav) is an indigenous cucurbit vegetable occuring in nature nearby cultivated areas on hedges or in open fields In Greek Solen means’ a tube,pipe’ refering to the nature of its flowers. The vernacular names are Bon-kundari, kudari, makal, mahakal (bengali), gometti (marati), kanduri, kuduri (bundelkhand), bankakra (champa), gometi (gujarati, anatmul, mahakal, taarali, kaduri (hindi),makirla (chattirgarh), kaage mari balli (kanarese) etc. This is a perennial vine, dioecious in nature. Tender fruits are used as salad, curry and  fried vegetable. It has significant medicinal value also.  In tribal regions of Orissa, fruits of Melothria are considered as delicacy. Visualizing the delicate nature of its fruits, it has great potential as a salad crop in  future.

Accurately in the clinical diagnosis of a disease in experimental animals frequently depends on the result of laboratory examination of blood specimen from the diseased animals. A prerequisite for good experiment result is the proper collection and handling of the blood samples. If properly collected samples can not be sent to the laboratory quickly. It must be preserved or stored in such a manner that the necessary tests may be conducted. The technician/researchers

Going to collect the blood from a laboratory animal should be aware of the purpose of blood collection, the sites of blood collection, the technique of blood collection, the Use of various anti coagulants, the techniques of blood examination.

Blood Examination of Animal may be Necessary

‘FRUIT’, “a food, drink and medicine, neatly packed by nature in attractive, handy and easy-to-open containers”, is a sensible definition indicating significance of fruits in human life. In botanical terms, fruit is a ripened ovary and in horticultural science it is the plant product, which is edible on ripening. From the nomadic age to present day civilized life, man has used fruits as food. Human civilization is irrevocably linked with the culture of fruit plants and trees. References of larges number of fruits in the very ancient literature of different languages throw light to this fact.

Fruits exhibit vast diversity in various forms, shapes and aspects. According to climatic requirements, they are temperate, tropical and subtropical crops. Major tropical and subtropical fruits include mango, banana, citrus, grapes, litchi, pineapple, papaya, sapota, guava, mangosteen, avocado, jack, ber, passion fruit etc. Fruits can be grown in very rich and favourable climatic conditions, but some fruits are adapted to adverse conditions of dessert (date palm), moisture-stress arid zones (aonla, ber, custard apple) and saline regions. Fruit plants vary in morphological traits such as plant size and shape. They may be herbaceous as banana and pineapple, shrubs as cherries and phalsa, small trees as ber and limes, big trees as mango and jack and vines as passion fruit and grapes. Fruits are of different sizes and shapes also. The range is from very small berries of phalsa to very big fruits of durian and jack. Taste wise variation is also very clear in fruits. We have very sweet fruits (litchi, sapota, dates and banana) acidic/sour ones (aonla, tamarind, bilimbi) and fruits with specific sugar/acid blend (varieties of mango, grapes, oranges).

Soil aggregates refer to a group of two or more primary particles which cohere to each other more strongly than to surrounding particles. Under field conditions, these units also cohere to some degree with neighboring aggregates and form larger aggregates. The aggregates form and break easily into smaller aggregates during tillage and by disruptive action of water and air. The aggregates maintaining their identity are those in which the cohesive forces among particles are greater than the disruptive forces. A quantitative characterization of aggregates is being done by determining aggregate stability and size distribution of aggregates. The aggregate stability refers to the resistance of soil aggregates to breakdown by water, air, and mechanical manipulations. Numerous methods have been proposed to determine size distribution and stability of soil aggregates (Kemper and Rosenau, 1986). The size distribution of wet and dry aggregates determines overall tilth, size of pores and susceptibility of aggregates to movement by water and wind.

Clinical Significance The primary aim of laboratory diagnosis is to assess the carbohydrate metabolism impairment in diabetes mellitus, inducing the following: 1. Determination of glucose concentration in fasting blood and demonstration of postprandial glycosuria. 2. Assessment of glucose tolerance

It is well known that organic matter plays a unique role in the binding of pesticides in soil, and that this phenomenon is usually the most important cause for interaction of pesticides in the soil environment. Fulvic or humic acids are most commonly associated with the binding interactions. Binding can take place with either the original pesticide or a transformation product, the reaction being induced by abiotic or biotic agents (microbial or plant enzymes). The reactions or processes involved are apparently the same as those responsible for the formation of humic substances, i.e., for the humification process.

Artificial insemination (AI) technology is one of the important managemental tools for the successful poultry industry. It has several uses and a few limitations also. This technique is performed to avoid the spread of venereal diseases by natural mating and to increase the dissemination of genetic material to a large number of birds. This technique include: semen collection, semen evaluation (physical and biochemical characteristics), semen dilution and preservation and deposition of semen in the female genitalia. Semen dilution is one of the most critical components of AI technology. Several suitable semen dilutors are reviewed for low temperature storage up to around 48 hours.