eChapters

Introduction to Open Broadcasting Software (OBS)
Open Broadcasting Software, commonly known as OBS, is an open-source platform designed for video recording and live streaming. It has become the software of choice for content creators, gamers, educators, and businesses due to its flexibility, powerful features, and ease of use. OBS is crossplatform, available on Windows, macOS, and Linux, and supports a range of applications from streaming live gameplay to creating professional-looking video presentations and tutorials.
Key Features of OBS
• Customizable Interface: OBS provides a highly customizable interface that allows users to arrange different elements like audio controls, scenes, sources, and transitions. This helps users create a personalized
workspace that suits their specific broadcasting needs.
• Multiple Source Types: OBS allows users to incorporate a variety of sources, such as screen captures, webcams, images, text, browser windows, and more, making it ideal for producing complex and visually
engaging streams.

Extraction of DNA from Tissue
(Using HiPurATM Multi-sample DNA Purification Kit)
Kit contents
Re-suspension buffer, Lysis solution, Prewash solution concentrate, Wash solution concentrate, Elution buffer, Proteinase K, HiElute Miniprep Spin Column (Capped), Collection tubes (uncapped), Collection tubes Dilute the prewash, wash solution and Proteinase K according to the kit.
Protocol
Prepare tissue
Weigh 25 mg of tissue, add approximately 3 ml of 0.8 per cent of normal saline. Homogenize the tissue with homogenizer or with mortar and pestle. Transfer the homogenized tissue with saline to a microcentrifuge tube. Centrifuge at 14000 rpm for 10 minutes. Discard the supernatant and use the pelleted tissue for further lysis.
Digest tissue
Add 180 μl of resuspension buffer and 20 μl of Proteinase K solution to the pelleted tissue in microcentrifuge tube. Mix by vortexing. Incubate the sample at 55oC until the tissue is completely digested (2-4 hours) in a shaking water bath. Vortex briefly after complete digestion.

Today it has been understood that yeasts have great potentials as novel probiotics (Nayak, 2011). They have been found to be safe for human consumption in traditional fermented foods (Jakobsen and Narvhus 1996). Yeasts have a great physiological importance inside the gastrointestinal (GI) tract (Czerucka et al., 2007; Gatesoupe, 2007). Jespersen (2003) has mentioned the role of consuming fermented foods and the health benefits attributed with it.Yeasts are known for prevention of table olive (Arroyo lopez et al., 2012). Bonatsau et al. (2015) have shown multi-functional property of yeast as starters.

Phalsa (Grewia subinaequalis) is one of the hardiest, drought resistant and quick growing fruit crop. Different parts of the plant are used for curing variety of ailments. The ripe phalsa fruits are consumed fresh, in desserts or processed into refreshing fruit and soft drinks enjoyed during hot summers.

Climate and Soil

Phalsa prefers dry conditions and can therefore, withstand drought. It flourishes well under variable climatic conditions, and requires protection from the freezing cold temperature. Also, adequate sunlight and warm or hot temperatures are required for fruit ripening, development of appropriate fruit colour and good eating quality. Kandi areas are, therefore, quite suitable for its cultivation. It can also be grown under irrigated conditions. Phalsa plant grows vigorously under wide range of soils including fine sand, clay or even limestone. However, best results are obtained in well-drained, loamy soils.

Very few traditional systems have a fair level of documentations in place. Most of the little traditions, the flow of knowledge is mainly through word of mouth, however, the great traditions such as Ayurveda and Traditional Chinese Medicine do have a good level of documentation. For instance, Ayurveda means science of life in Sanskrit and aims at holistic management of health and disease. It remains one of the most ancient medical systems widely practiced in Indian subcontinent and has a sound philosophical, experiential and experimental basis541. Charak Samhita and Sushrut Samhita (100-500 B.C.) are main Ayurvedic classics, which describe of over 700 botanicals along with their classification, pharmacological and therapeutic properties542,543. A classic diagnostic text Madhav Nidana includes over 5000 signs and symptoms. During the course of evolution and years actual practice, the interpretation of such documents developed different schools of thoughts and resulted in wide variations in understandings. Similar situation also exists in other systems including TCM. These differences often raise issues related to authenticity and correctness of Traditional Knowledge (TK). Thus systematic documentation, interpretations and harmonization of concepts and practices remain a major challenge in most of the systems of TM. Many governments, professional and community organizations have undertaken such documentation and harmonization exercises. Projects like Traditional Medicine Knowledge Digital Library, AyuSoft, Triskandha Kosha and Medicinal Plants Database of FRLHT are attempting systematic documentation of Ayurveda in India544. Other databases such as The NAPRALERT (NAtural PRoducts ALERT) developed by Professor Norman Farnsworth contain bibliographic and factual data on natural products, including information on the pharmacology, biological activity, taxonomic distribution, ethno-medicine and chemistry of plant, microbial, and animal (including marine) extracts. Another project relates to the Global IP, Benefit Sharing and Traditional Medicine Database. It is being developed as a component of the Global Information Hub on Integrated Medicine from the work of the Commonwealth Working Group (CWG) on Traditional and Complementary Health Systems, now directed by the Malaysian Ministry of Health. This Information Hub is planning to create a network of legal centers, scholars and NGOs working together to develop a comprehensive legislative and policy review, information resource and exchange on issues pertaining to IP rights over traditional medical knowledge and the use of medicinal plants545. Other than such efforts, there is limited systematic documentation of TM knowledge and practices.

10.1 Introduction

Plant breeders come across two categories of characters (traits) for improvement, namely, qualitative characters and quantitative characters. Red vs white flower, round vs wrinkled seed, determinate plant growth vs indeterminate plant growth etc. are examples of qualitative trait differences. There is no overlapping of characters and no confusion during classification. Such trait differences are usually governed by one or two gene pairs, each with large effect that can be measured even in the presence of segregation at other loci and even in the presence of effects of environmental factors. These characters are very less influenced by the environmental factors.

On the other hand, most of the traits of economic importance like yield of fruits of vegetable and fruit crops, yield of vegetative parts in fodder crops, yield of lint in cotton, yield of grains in cereals, pulses, oilseeds etc., plant height, days to maturity etc. are examples of quantitative characters. Variation observed for such traits is continuous. Again, there is overlapping of trait differences. So, one cannot assign individuals to a specific class unambiguously. Such characters are influenced by environmental factors, such that phenotypes are not same when grown over locations and/or seasons.

When different plants/lines of a species exhibit trait differences, it is called variation. Variation is essential for any improvement in a crop. It may be present in the population to be improved or else it may be created by different means such as hybridization, mutation, polyploidy, domestication, plant introduction, somaclonal variation, genetic engineering etc. Now, we shall discuss the types and estimation of variation.

In order for a plant to become diseased, three conditions must be present: (1) a pathogen, (2) a favorable environment and (3) a susceptible host. All the strategies we use to manage plant diseases work to remove or limit one of these factors, thus breaking the plant disease triangle. The key to sustainable plant disease management is to establish an agro- ecological system that is favorable to plant growth and development at the population level and adverse to pathogen evolution and epidemic development based on interactions among plants, pathogens, vectors and environment.

Duration: 60-75 minutes
Objective: Develop creative and analytical skills to identify, analyze, and resolve workplace challenges effectively.
Why It Matters
Problem-solving is critical for overcoming obstacles, improving processes, and driving innovation. Effective problem-solvers think critically and act decisively, while poor problem-solving leads to delays or ineffective solutions. For example, misdiagnosing a client complaint can escalate issues, while a structured approach resolves it quickly. This module equips participants with practical tools to tackle problems confidently.
Learning Outcomes: By the end of this module, participants will:
1. Understand a structured process for problem-solving (define, generate, evaluate, implement).
2. Practice root cause analysis and brainstorming techniques.
3. Apply problem-solving strategies to real-world scenarios.

9.1 Introduction
The term “pasteurisation” is derived from the Frenchman Louis Pasteur, who demonstrated that the preservation of wine was enhanced by heating it to a temperature of 122 to 140 °F, which eradicated spoilage organisms. This occurred between 1860 and 1864. The utilisation of this procedure resulted in the development of a new term, “pasteurisation,” which rapidly acquired popularity in the field of technology. Although Louis Pasteur was a pioneer in the discovery of the use of thermal treatment to preserve wine, the process of pasteurising milk was initially attributed to Dr. Soxhlet of Germany in 1886.

9.2 Heat treatment of milk
In the context of market milk, pasteurisation is currently defined as the process of heating each component of milk to a minimum of 63°C for 30 minutes, 72°C for 15 seconds, or any other temperature and duration that achieves the same level of effectiveness. Equipment that has been certified and functions properly is required for this process. The milk is promptly chilled to a temperature of 5°C or lower after the pasteurisation procedure.

In India, the collection, transport and distribution of raw milk involves a number of agencies and persons at rural and urban level – village producers, milk collectors, halwais (Sweet-makers), milk vendors, dairies, producer-retailers and wholesale or retail markets.

1. Collection

Usually, the milk collectors approach the small village producers to collect milk and the same is sold directly or indirectly (through agents) to a dairy (milk procurement centres) or halwais or city milk vendors or consumers. The producer – retailers themselves, in some cases, supply the milk to the above said parties. The production of milk in small, scattered holdings in village causes problems during its collection. It involves visits to many villages, some of which are not easily accessible for the purpose.

Atuna racemosa subspecies racemosa is shown to be a species with many uses throughout its range from Malaysia to Melanesia. The principle uses described are the seed oil used as anti-inflamatory massage oil and for hair dressing, the seed pulp that is used for caulking and varnishing boats, the wood for house construction, and many parts of the plant as ingredients of local medicines. The medicinal uses of this species deserve further study since it has been shown to have both anti-bacterial and anti-inflamatory action. It is also uncertain whether its distribution over the Pacific is natural or human caused.

Atuna racemosa Rafin. is a widely distributed tree species that ranges from the Malay Peninsula through Malesia, Australasia and to the Pacific islands as far as Fiji, Tonga and Samoa. As with many useful plants, its natural distribution is uncertain because it has been transported around from island to island during the various migrations of the Pacific islanders.

Pecan (Carya illinoensis) is a highly nutritive and energy giving nut fruit. It has nutty flavor and superb tree characteristics. Area under pecannut in J&K is 414 hectares with annual production of 86 metric tonnes.

Climate and Soil

Pecan need warm temperate climate and extremes of both temperate and sub-tropical climates are undesirable. It needs adequate chilling hours ranging from 400-600 hours below 7.2^oC for different cultivars. Mean monthly temperature ranging from 24.0 to 29.5⁰C, with little diurnal variation during growing period is best for growth and fruiting. High humidity prevents pollination, increases the incidence of diseases and premature splitting of nuts.

Although pecan nuts are grown in varied soil conditions, but sandy loam soils (pH 5.0-8.0) which are deep, fertile and are rich in organic matter are best for its growth and fruiting. Symbiotic mycorrhiza and fibrous roots permeate the top 1 meter of soil.

Drying

Drying is one of the age-old techniques for preservation and value addition of food products. Generally, it means removal of moisture from the food products by evaporation. It is aimed at lowering the water content of food stuff, thereby arresting the growth of microbes, action of enzymes and other autolytic chemical reactions (Naidu et al., 2016). This preservation technique is predominately used for food materials which are regarded as “highly perishable”. The benefits of drying includes extended shelf-life, lower storage space, reduced packaging requirements, lower handling and transportation costs, off-seasonal availability and importantly diversified product for the ultimate consumers (Bonaui et al., 1996).

Fish drying

Open sun drying as a method of fish preservation is recorded in history by the primitive societies. However, open sun drying of fish has few draw backs which includes poor product quality, higher microbial contamination and complete dependence of weather conditions for drying (Bala & Mondol, 2001). In the later stages, drying developed into completely a controlled process in which drying air temperature, relative humidity and air velocity were precisely maintained at optimum level. The fish drying process is slightly different from other food materials mainly due to its gel like behaviour until considerable reduction of moisture. Moreover, significant amount of shrinkage takes place in fish during drying in addition to the other irreversible changes.

Diagnostic Symptom

• Circular to oval, dark brown to black spots appear on the leaves.The spots enlarge into round to irregular spots with concentric rings and coalesce causing blighting and withering of leaves.

• These are surrounded by chlorotic zone with grey white necrotic center.

Of late, there has been a growing trend for individuals and companies to “Go Green” or be more environmentally friendly. There has also been a opinion that natural and organic products are healthier or safer. The pest control industry has responded to these changes in our culture and business markets with innovations in products, materials, and methods. Requests for “organic” based services have grown to a point where most pest control providers offer services that use only natural pesticides.

Cardiomyopathies in acts can be classified broadly as Hypertrophic cardiomyopathy and non-hypertrophic cardiomyopathies. Usually, no alarming clinical signs are detected during routine examination in the preclinical stage except cardiac murmurs in some cats with HCM. This is because, that the cats are sedentary and excellent in hiding the signs of illness from the pet parents. Hence the onset is often acute with the sudden appearance of the clinical signs linked to congestive heart failure like severe dyspnoea, cardiogenic shock, aortic thromboembolism, arrhythmias and even syncope and death

Cats with CHF will be presented with severe dyspnoea, due to fulminant pulmonary oedema and or pleural effusion. The pleural effusion associated with CHF is usually a modified transudate, some cases it may be chylous .In echocardiography ,the size of the left atrium should be determined .If the LA size is normal ,then the dyspnoea may not be of cardiac origin .The presence of B-lines in the lung area in association with LA enlargement confirms cardiogenic pulmonary oedema. Stress, anaesthesia, I/V fluids and steroids leads to decompensation and fulminant pulmonary oedema. Thoracocentesis is will be helpful if there is dyspnoea due to significant pleural effusion. At least 300ml of effusion can be usually removed. The dyspnoeic cat is very fragile and should be handled carefully. Butorphanol may be given for anxiolytic effect. Oxygen administration is beneficial. Furosemide when given intravenously has an initial venodilator effect and should be repeated as required monitoring the sleeping respiratory rate (SRR). Addition of nitro-glycerine (vasodilator) reduces preload, decreases left atrial pressure and help in reducing pulmonary oedema. If the clinical signs are suggestive of cardiogenic shock or myocardial failure, Pimobendan I/V or Dobutamine CRI should be considered.

Introduction
Chamomile is generally known as German or Hungarian chamomile. Its herb is commonly used for both medicinal as well as aromatic purposes. It was introduced in India during late fifties by Regional Research Laboratory, Jammu (Handa et. al., 1957) and subsequently in different parts of India. It was successfully grown over saline soils at CIMAP, Lucknow and later at several other locations. It was also introduced at YSPUH&F at Solan (H.P.) under AICRP on M&APs and several parameters were identified for better utilization of inputs for higher production of herb. Its flowers also contain an essential oil with multiple uses.
Origin
It is native to Europe and mainly cultivated in the regions of northern, central and eastern European countries, Mediterranean, N. Africa, United States, Germany, France, Hungary and Russia. It is found grown in wild state in
eastern and southern Europe, West Asia and North Africa.

Introduction
This chapter discusses the crucial final stage of any video or multimedia production: the presentation. A well-produced program needs to be presented effectively to captivate, inform, and engage the intended audience. This chapter explores various facets of presenting a produced program, including preparation, techniques for impactful presentation, adapting to the platform and audience, and post-presentation considerations.
Understanding the Importance of Presentation
• Setting the Tone: A well-presented program captures the essence and quality of the production. Presentation directly influences how audiences perceive the program’s message, style, and relevance.
• Role in Communication: Presentation serves as the final link between the production team and the audience. It ensures the message is not only delivered but also absorbed and remembered.
• Branding and Identity: The presentation also reflects the program’s identity and the reputation of the production team or organization, affecting brand perception and credibility.

Background
As a non-invasive and relatively stress-free method, saliva holds great potential as a diagnostic sample. It has already been utilized in diagnostic tests for rabies and other diseases in dogs. Saliva secretion is the secretion of salivary glands that maintains homeostasis in the oral cavity, protects against pathogens, and helps digest food. It contains inorganic substances, cells (blood and epithelial cells) and microorganisms. Saliva also contains immunoglobulins, enzymes, hormones, and cytokines derived from serum and bronchial secretions. The saliva of dog is being used to detect rabies virus (Kasempimolporn et al., 2000), parvo virus (Kocaturk et al., 2021), cancer and measure stress levels. Obtaining saliva rapidly, painlessly, and uncomplicatedly can be an easy tool for disease screening.
For the identification of serval prognostic biomarkers for various physiological and pathological conductions, saliva proteomics can be very helpful. In humans, saliva contains more than 3000 proteins (Kawas et al., 2012). Several diseases like oral carcinoma, AIDS, hepatitis B, cystic fibrosis, and other diseases like diabetes mellitus. Differential expression of dog saliva proteins compared to human saliva revealed 2532 proteins. Proteins related to apoptosis and adhesion were predominant in the oral proteome of dogs, in addition to proteins related to regeneration and healing processes such as FGF and EGF (Sanguansermsri, et al., 2018).

Overview of Soil Erosion Conservation Measures