eChapters

8.1 Introduction

Electron Microscopes were developed due to the limitations of Light Microscopes which are limited by the physics of light to 500x or 1000x magnification and a resolution of 0.2 micrometers. In the early 1930’s this theoretical limit had been reached and there was a scientific desire to see the fine details of the interior structures of organic cells (nucleus, mitochondria etc.). This required 10,000x plus magnification which was just not possible using Light Microscopes. The Transmission Electron Microscope (TEM) was the first type of Electron Microscope to be developed and is patterned exactly on the Light Transmission Microscope except that a focused beam of electrons is used instead of light to see through the specimen. It was developed by Max Knoll and Ernst Ruska in Germany in 1931. The first Scanning Electron Microscope (SEM) debuted in 1942 with the first commercial instruments around 1965. Its late development was due to the electronics involved in scanning the beam of electrons across the sample. Electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology, medicine and material sciences. This wide spread use of electron microscopes is based on the fact that they permit the observation and characterization of materials on a nanometer (nm) to micrometer (µm) scale. Electron Microscopes are scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale. This examination can yield information about the topography (surface features of an object), morphology (shape and size of the particles making up the object), composition (the elements and compounds that the object is composed of and the relative amounts of them) and crystallographic information (how the atoms are arranged in the object). A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that can be detected and that contain information about the sample surface topography and composition. SEM can achieve resolution better than 1 nanometer. The combination of higher magnification, larger depth of focus, greater resolution, and ease of sample observation makes the SEM one of the most heavily used instruments in research areas today.

According to Pasteur statements which one of the following is true

a) Living organisms discriminate between stereo isomers

b) Fermentation

c) Living organisms doesnít discriminate

d) Both a and b

Theory

Research: The word research is composed of two words “Re” and “Search”.
“Re” - ‘Again or over again or a new’ 
“Search” - ‘To examine closely and carefully’ or ‘to test and try’. 
Research is a careful, systematic, patient study and investigation in some field of knowledge undertaken to establish principles / policies. 
Science: It is a systematized knowledge derived through observation, experimentation or any other systematic method or procedure in order to determine the nature of phenomenon or process being studied or the causes behind specific events.

Carbohydrates are the most abundant biomolecules on earth. They occur in nature as glucose, starch and cellulose etc. Each year, photosynthesis converts more than 100 billion metric tonnes of carbon dioxide and water into starch, cellulose and other plant products. Carbohydrates are polyhydroxy aldehydes or ketones and their derivatives. They provide 4k calories energy per gram. They promote utilization of lipids and reduce wastage of proteins. They are readily available in roots, tubers, cereal grains, sugarcane and sugar beet.

Carbohydrates have the general formula C_n( H₂O)n, indicting that they are the hydrates of carbon. Some carbohydrates also contain nitrogen, phosphorous or sulfur. They are characterized by the number of carbon atoms they contain. If n=3, they are trioses, n=4 tetroses, n=5 pentoses, n=6 hexoses and n=7 heptoses. The most common sugars in plants are the hexoses. Two or more simple carbohydrates condense together to form polymer sugars like sucrose, starch and cellulose etc. The basic condensing bond is glycosidic bond.

4.1 Introduction

A huge amount of waste in the form of liquid and solid is produced in the fruit and vegetable processing industry which causes pollution problem if not utilized or disposed off properly. Generally solid waste is dumped in local landfills, eaten and scattered by stray animals, which has now become a major cause of environmental pollution. The waste obtained from fruit processing industry is extremely diverse due to the use of wide variety of fruits and vegetables, the broad range of processes and the multiplicity of the product [79]. The solid waste from fruits and vegetables includes peel, stone, rag, seed, core etc is of economic value and can be used for manufacturing of various by-products [21]. The extent of the waste produced from processing industries of some of the important fruits and vegetables, is given in Table 1.

In India, utilization of fruits and vegetables for processing is hardly 1.8% of the total production. There were 5166 processing units till 1^st Jan, 2009 of fruits and vegetables products throughout the country. About 90% of these units are small scale units with annual production limit of 50-250 tonnes. These units have no organized methods of waste disposal. In certain places this waste is taken away by farmers for their disposal as cattle feed or field manure.

Important Points to Remember

ROSE

• Botanical name: Rosa spp.
• Family: Rosaceae
• 2n = 14
• Origin: North Hemisphere (Himalyas)
• Flower colour: Red (commonly)

Introduction

Technical innovation is a process by which new or improved technologies are developed and brought to widespread use (Ambuj Sagar, 2013). Technical innovations are interlinked by research, development, demonstration and deployment which involves a range of organizations and personals with institutional arrangements underpinning the growth and advancement of different kinds of technologies. In the area of water productivity, technical innovations has helped expand water supplies through improved exploration and recovery techniques, increased water use efficiency, improved water availability and reduced environmental impact of water and its usage.

Introduction

Diffusion is the process by which an innovation is communicated through certain channels over time among the members of a social system (Rogers, 1962, 1983, 1995, 2003). It is a broader term, which encompasses unplanned as well as planned and directed spread of an innovation. Dissemination is planned and directed diffusion of an innovation. In case of Integrated pest management which is a complex sort of decision making process, many technology transfer approaches were/are tried for dissemination of IPM technologies to farmers. These include radio, television, print media, training and visit, field days, demonstrations, extension field visits and participatory learning approaches like participatory technology development (PTD) and farmers field school (FFS). While Training and Visit (T&V) system failed, Farmers Field School (FFS) approach of educating farmers followed by ‘Community IPM’ activities and ‘Farmers to Farmer’ approach registered success in IPM implementation in developing countries (Matteson, 2000).

Introduction

India ranks second in the production of vegetables after China. The existing area under vegetable cultivation in India is around 4.5 million ha. There is a need of around 5-6 million tons of food to feed 1.3 billion India’s population by 2020 AD. The major constraint in vegetable production, include the extensive crop losses due to increased pest infestations directly or due to viral diseases vectored by insects. The extent of crop losses in vegetables varies with the plant type, location, damage potential of the pest involved and cropping season. Vegetables are more prone to insect pests and diseases mainly due to their succulence as compared to other crops. The crop losses to the tune of 40 per cent have been observed in vegetable crops. The increased demand and limited productivity of local cultivars, led to introduction of high yielding varieties and hybrids. As a result, dramatic changes in pest scenario occurred like minor pests assuming major status (eg., Serpentine leaf miner, Liriomyza trifolii a serious problem in tomato hybrids). Chemical pesticides are the sole panacea to mange the pest by vegetable farmers even today. Indiscriminate use of pesticides has led to severe ecological consequences like destruction of natural enemy fauna, effect on non-target organisms, pesticide residues in vegetables and resistance of the pest to pesticides. The modern pest management is aimed at educating the farmers on various issues of pest management like identification of the pests, natural enemies, judicious use of pesticides with alternate methods of pest control like cultural, mechanical, biological and behavioral (pheromones) management.

Network in Project Management

A project network is a graph that shows the activities, duration, and inter dependencies of tasks within our project.

Networking is a crucial aspect of project management that can provide various benefits, including access to new job opportunities, industry insights, and professional development opportunities.

What is network with diagram?

A Brief History of Project Management.

Introduction
The method of selective breeding involves selecting particular breeding prospects to accumulate beneficial alleles and increase the prevalence of desired traits in a population. Characteristics, including growth, disease resistance, reproduction, development, and coloration, are frequently addressed in aquaculture (Maluwa, 2022). Selective breeding, whereby people are picked to have offspring with desired traits, is the foundation of genetic improvement initiatives in all organisms. This is a type of directed evolution in which the breeder determines fitness instead of the organism’s innate capacity for survival and procreation. Darwin’s theory of natural selection was affected by selective breeding, which existed before the mechanics of heredity were discovered. Darwin agreed that deliberate human selection used excellent outcomes (Hill, 2017). By choosing better parents, a species’ desirable features can be improved. Common carp (Cyprinus carpio) were first cultivated in ponds in China 4,000–5,000 years ago, marking the beginning of fish farming. Environmental effects, growing competition for water, and increased demand due to population development and declining wild fish sources are some of the issues aquaculture faces as it grows. Controlled mating and lineage monitoring were two early breeding techniques that resulted in severe inbreeding and decreased output. Mendel’s plant experiments established the groundwork for quantitative and contemporary genetics when they were made public in
1900 (Gjedrem and Baranski, 2010). Mendel’s experiments were confirmed in 1900, marking the beginning of selective breeding for plant production. Fifteen years later, Farm animals were treated using the same methods (Hagedoorn, 1950). Genetically modified stocks are used for most plant and farm animal production. Genetic advancement in aquaculture is still sluggish, though. Although the first aquaculture family-based breeding program began in 1975, by 2010, only 8.2% of aquaculture production was based on genetically modified stocks (Neira, 2010; Rye et al. 2010).

The world turned into a grossly inhabitable during the infamous COVID-19 pandemic, with community health issues everywhere. Each one felt unsafe if other earthly inhabitants were, be it humans or other living beings. Onset of SARS CoV-2 set a ‘new normal’ world order. The ever-evolving and mutating SARS CoV-2 variants extended the pandemic wave after wave thereby complicating the situation, pushing the global healthcare system to its limits and exposing its underbelly. The continually evolving variants were more aggressively transmissive and infective. As the world still reels under tremendous healthcare pressure, other infectious diseases are emerging as are identified by the World Health Organisation (WHO), creating international health emergency situations. The situation becomes alarming with the increasing zoonotic (spillover) and reverse zoonotic (spillback) factors being more proactive in the disease spread. There is a greater need to vaccinate not only humans but also animals, especially the domesticated ones and known reservoirs/carriers. Vaccines must be developed on priority and equitably distributed as the first step to counter a pandemic or potential pandemic. Vaccination is self-limiting due to increasing reported breakthrough infection cases. A strong link of the environmental health to the overall global health needs to be acknowledged, and due diligent measures taken. Hence, there is a strong need of the One Health approach as a 360° solution, and follow it up at global-scale both in letter and spirit. This chapter discusses spillover and spillback of diseases like SARS-CoV-2 and Mpox as case examples, the mitigation strategies and the need for workable One Health models, and challenges and measures to counter future outbreaks. It stresses on foolproof vaccination strategies across to catalyse individual and herd immunities for universal wellbeing.

Introduction

Spatial data is different from a non-spatial data in the sense that it always has a spatial reference system which is nothing but a pair of coordinate values and a system of reference for these coordinates. For example, the locations of benchmark soil series of India can be defined as pairs of longitude and latitude in decimal degree values with respect to the prime meridian at Greenwich and zero latitude at the equator. The World Geodetic System (WGS84) is a frequently used system of reference on the Earth. The different types of spatial data are: (i) point, which is a single point location, such as a GPS reading or a geocoded address (ii) line, which is defined as a set of ordered points, connected by straight line segments (iii) polygon, an area, marked by one or more enclosing lines, possibly containing holes and (iv) grid, a collection of points or rectangular cells, organised in a regular lattice. Among the above said spatial data, the first three are vector data models and represent entities as exactly as possible, while the fourth data model is a raster data model, representing continuous surfaces by using a regular tessellation. In this chapter, we discuss how a spatial data can be handled in R. There are several packages available in R in which spatial data analysis can be handled e.g. sp, rgdal, raster etc. All the contributed packages for spatial data analysis in R address two broad areas: moving spatial data into and out of R, and analysing spatial data in R. In the following section, the sp package for spatial data handling is briefly discussed.

Floriculture is the most important aspect of urban agriculture, which fulfils the need for beautification, recreation, entertainment and income earning. In broader sense, it includes growing flowering and ornamental plants in gardens, rooftops and terraces. Floriculture comprises of house plants, flowering plants, pot plants, cut flowers and decorative foliage. It also deals with cultivation of cacti, orchids, succulents, bonsai, ferns and plants grown in landscape gardening.

Role of flowers in our life

• It is a unique gift of nature to relax human mind
• The fragrance and beauty of flowers bring joy and happiness
• Flowers are used as a symbol of grace and sophistication
• Flowers are used in religious rituals for offering during worship

Enzymes are organic catalysts produced by living organisms. They make possible biochemical reactions. Hence they are called biocatalysts. They are mostly a specialized class of proteins. However, there are some non-protein enzymes like ribozymes, which are RNAses. Enzymes speed up reactions at ambient temperatures. This is in contrast to chemical reactions like saponification of lipids which take place when lipids are boiled for a few hours with concentrated alkali.  Enzymes, namely, lipases, hydrolyze lipids at body temperatures in minutes. They are superior to chemical catalysts by a factor of 107 to 1014 .  An enzyme may be a simple protein or a complex protein. They are produced by cells and are mostly globular proteins. Each cell contains an estimated 3000 different enzymes.

Fruit trees can be planted in the backyard or any open space near the house. In absence of backyard area, fruit trees can also be grown on the rooftop by planting in polythene bag, plastic container, cemented pot, etc. Growing fruits like papaya, banana, lemon, mango, pineapple, coconut, pomegranate, etc. in the premises of house can fulfil the family requirement of fruits. It will help to get fresh fruits from own garden, which are free from any chemical or pesticide. There is a different satisfaction in plucking of fruits grown by own effect.

Mango

Mango (Mangifera indica) is one of the prominent fruit crops of India. It has delicious taste and excellent flavour. Mango fruit is utilized at all the stages of its development. Raw fruits are used for preparation of pickle, chutney and juice. The ripe fruits are consumed directly and are used for preparation of several products like squash, syrup, jam and jelly. Ripe mango contains about 0.6 per cent protein, 0.1 per cent fat, 11.8 per cent carbohydrate, 0.3 per cent minerals and 1.1 per cent fibre. 

Introduction

Potato is mostly considered as a vegetable crop in India, whereas it is a staple food crop in Europe, Canada, Australia, U.S.A, South America, Russia, Asia and other countries. In India, it is cultivated extensively in the hilly tracts during December-March or March-June and in the plains during September-January, as it requires cool and moist climate condition. As per FAOSTAT (2015), the potato production in India during 2013 was 45.34 mt from 1.99 mha area with a productivity of 22.76 t/ha. There are several varieties of potato grown in India and many diseases, some of which cause considerable damage to the crop. Potato crop can be affected by diseases caused by fungi, bacteria, virus, viroids and others by non-parasitic.

Late Blight

Economic Importance: Late blight is one of the most devastating diseases of potato throughout the world. It is recorded in area with frequent cool and moist weather. The disease is believed to have been introduced into Europe from South and Central America and spread to other countries which include the East Coast of Canada, Western Europe, Central and Southern China, South Eastern Brazil and the tropical highland. It created history by causing a famine in Ireland known as Irish Famine during 1845-46 resulting in death of many people due to starvation, dysentery and typhus spread among the farmers and subsequent migration of people from Europe especially from Ireland. Late blight has the tremendous potential to cause upto 70% reduction in the yield in a susceptible cultivar. Region wise economic importance of late blight shows that the disease takes highest toll of potato in Sub-Saharan Africa (44% crop losses) followed by Latin America (36%), Caribbean (36%), South-East Asia (35%), South-West Asia (19%)

• The proportion of bone varies depending on breed and body condition from 12 to 28% in the dressed carcase and approximately 15% in a good large ruminants carcase
• Bone percentage increases with the age and weight of the animal and it is lowest in Aberdeen Angus cattle and it may increase to 28% in low quality large ruminants
• The average bone % in bobby calves is 50%, veal calves 25%, in lamb 17–35%, in pork 12–20% and in poultry 8–17% Important parts of digestive system of meat animals Ox tongue
• Filiform papillae found on the tongue are pointing backward and horny and thus, helps in food prehension
• Actinobacillosis erodes the prominent posterior part of the tongue dorsum
• 10–14 circumvallate papillae are spread in the region of prominent dorsum around the either side of the tongue midline
• Black pigmentation of the tongue surface is fairly normal and not associated with any disease condition
Sheep and goat tongue
• Similar to cattle tongue
• However, papillae are not horny but the centre of the tip is slightly grooved
• Black pigmentation of the surface of the tongue is common in blackskinned sheep.

ABSTRACT

Dysoxylum binectariferum (Roxb.) Hook. is an evergreen tree, indigenous to India and widely distributed in different parts of India. Primarily, this plant is used in the timber market for the preparation of furniture and other wooden goods. Apart from utility in timber market, it has tremendous medicinal properties because of its unique secondary metabolites. Its hydroalcoholic extracts possess a wide range of pharmacological activities including immunomodulatory, anti-inflammatory, anti-cancer, anti-leishmanial, etc. The chromone alkaloid rohitukine, the major constituent of the plant, possess anti-fertility, anti-leishmanial, anti-adipogenic, anti-ulcerogenic, anti-cancer and immunomodulatory activities. The most extensively studied and advanced therapeutic property of this chromone alkaloid is its cyclin-dependent kinase inhibition activity, which has a direct link with its anti-cancer effect. Inspired by this natural product, two clinical candidates (flavopiridol and riviciclib) are discovered and studied in human cancer patients for treating various types of cancer. Another preclinical lead IIIM-290, which is directly derived from rohitukine via synthetic modification, has demonstrated promising oral efficacy in animal models of various cancer types. The high-value medicinal applications of this plant have necessitated the need of bringing this tree under captive cultivation. The present chapter discusses the biology pharmacological and secondary metabolites present in Dysoxylum binectariferum. The preclinical and clinical leads derived or inspired from rohitukine are also discussed in this communication.