eChapters

Objectives

• To study working principles of simple microscope
• Study different parts of simple microscope

Introduction

This is the first ever microscope. It was founded by Leeuwenhoek in 17^th century. He combined a convex lens with a specimen holder, for seeing the magnified images of small objects. It looks like a magnifying glass because it has the capacity to magnify between 200 and 300 times. A simple microscope is a small focal lens used to view the magnified images of small objects. It is just like a hand lens and glass with relatively lower magnification power.

Natural farming provides a holistic, sustainable approach to agriculture that aligns with natural processes while offering significant ecological and economic benefits, especially within the Indian agricultural landscape.
Natural farming rejuvenates soil health by eliminating synthetic fertilizers and pesticides. Instead, it relies on inputs like desi cow dung and urine, mulching, and fermented bio-concoctions such as Jeevamrit. These inputs enhance soil organic matter, improve microbial diversity, and support natural nutrient cycles. As a result, soils under natural farming retain water better, allow for proper aeration, and are more fertile and resilient to climate-induced stressors like drought and floods.
The practice also promotes biodiversity. Without chemical runoff, ecosystems support pollinators, birds, and beneficial insects. Techniques such as intercropping, trap cropping, and the use of native seeds further balance the agro-ecosystem, naturally controlling pests and fostering long-term ecological harmony.
Economically, natural farming significantly lowers input costs. Farmers avoid purchasing costly synthetic chemicals and genetically modified seeds, instead utilizing affordable, farm-made formulations like Neemastra and plant-based repellents. This reduces market dependency and increases self-sufficiency.
States such as Andhra Pradesh and Himachal Pradesh have seen positive economic shifts where natural farmers report reduced debt levels and improved net incomes, Although yields may be marginally lower, the drastic reduction in input costs ensures better profitability.

Brain

The brain, a command centre of the body, is a complex organ. The substance of the brain is made of the grey matter and the white matter. The grey matter is often identified as cerebral cortex but other parts of the brain also contain the grey matter. Inside the brain, the grey matter is organized into large accumulations of neurons which are called the nuclei of the brain. Each nucleus is associated with a specific function. In addition to neurons, there is other type of cells the glial cells called neuroglia. The neurons are responsible for sending and receiving impulses or signals. The glial cells are nonneuronal and provide support and nutrition to neuronal cells, form myelin and facilitate signal transmission in the nervous system. The white matter is made of millions of nerve fibres that are coming from neurons and are going to establish communications with different parts of the brain and spinal cord. Since these nerve fibres are coated with myelin sheath around them these appear white and the area occupied by these nerve fibres become the white matter of the brain.

The brain has three basic components, the forebrain, midbrain and the hindbrain.

Fore brain: The forebrain (Prosencephalon) is the rostral portion of the brain. It consists of cerebrum (Telencephalon) and the thalamus, hypothalamus and pineal gland together constituting the interbrain (Diencephalon).

Mid brain: The midbrain (Mesencephalon) is the middle portion of the brain. It is in the centre lying between the interbrain and hindbrain. It consists of a cranial part of brain stem.

Hind brain: The hindbrain (Rhombencephalon) is the caudal most portion of the brain. It consists of remaining (caudal) part of the brain stem, the medulla oblongata (Myelencephalon) and the cerebellum and pons (Metencephalon).

Horse

The first cervical vertebra (atlas) of horse is more or less similar to that of ox with a few variations as below

The wings of atlas are curved ventrad and laterad. These are thinner than in ox but their borders are thick.

The wings present an additional foramen, the transverse foramen in the caudal part.

A faint groove on the ventral surface of the wing connects the transverse foramen to the intervertebral foramen.

(Method of Hyden 1955a,b)
I. Use of Polyethylene Glycol (PEG)

These compounds are manufactured through the reaction of ethylene oxide with water, ethylene glycol or diethylene glycol to furnish functional  groups (in this case hydroxyl) for the propagation of the reaction. The process results in a mixture of diols of different chain lengths, in which the numbers of molecules of various sizes are presumed to be represented by Poisson’s distribution (Shaffer, Critchfield and Nair, 1950b). Polyethylene glycols 200, 300, 400 and 600 are fluids: compounds 1,000, 1,500, 4000, 6000 and 1,0000 are solids of increasing firmness. PEG compounds of molecular weight above 1000 are not absorbed from the gastrointestinal tract of the rat and man.

Polyethylene glycol has been used frequently as a water soluble marker in studies of absorption in man and animals (Sperber et al., 1953; Hyden, 1955a,b; Corbett et al., 1956; Gray et al., 1960; Weller et al., 1962; Ulyatt, 1964a,b; Walker and Hawley, 1965; Tulloh et al., 1965; Sinha et al., 1970; Nagel and Piatkowshi, 1972).

Aim

To know the method of rehabilitation of downed animal within exercise wheel cart.

Indication

Irreparable trauma to vertebral column,permanent paralysis, downed animal.

The water contents expressed under certain standard conditions are commonly referred to as soil moisture constants. They are used as reference points for practical irrigation water management. The usage of these constants together with the energy status of soil water gives useful knowledge. These constants are briefly explained below:

6.1 Saturation Capacity

Saturation capacity refers to the condition of soil at which all the macro and micro pores are filled with water and the soil is at maximum water retention capacity”. The matric suction at this condition is essentially zero as the water is in equilibrium with free water. Excess water above saturation capacity of soil is lost from root zone as gravitational water.

Growth: Growth involve cell division subsequently cell elongation. Increase in volume that is associated with fruit growth largely result of cell division or cell elongation or both. In some fruit such as apple, extension of intercellular space may also contribute the growth. Generally growth by cell division in early stage whereas, cell elongation during later stage.

Two distinct type of growth curve are observed when increase in such variable such as fresh weight, volume and dry weight of fruit.

1. Single sigmoid curve
2. Double sigmoid curve

In case of single sigmoid growth curve firstly there is rapid increase in size then after decline so there is only one rapid growth is observed e.g. Apple, pear, tomato, cucumber and strawberry.

In case of double sigmoid two rapid growth period are separated by an intermediate period that is less growth or no increasing growth curve. Double sigmoid growth curve can be used as two successive sigmoid curve thus there are three clearly define stage of growth.

Enzymes are biocatalysts that change the rate of reaction without being changed themselves. All (Most) enzymes are proteins but all proteins are not enzymes. Ribonuclease is the enzyme which is non- protein in nature.

Properties of Enzymes

Crop water requirement is the water required by the plants for its survival, growth, development and to produce economic parts. This requirement is applied either naturally by precipitation or artificially by irrigation. Hence the crop water requirement includes all losses like:

• Transpiration loss through leaves (T)

• Evaporation loss through soil surface in cropped area (E)

• Amount of weather used by plants (WP) for its metabolic activities which is estimated as less than 1% of the total water absorption. These three components cannot be separated so easily. Hence the ET loss is taken as crop water use or crop water consumptive use.

• Other application losses are conveyance loss, percolation loss, runoff loss, etc., (WL).

• The water required for special purposes (WSP) like puddling operation, ploughing operation, land preparation, leaching, requirement, for the purpose of weeding, for dissolving fertilizer and chemical, etc.

• Hence the water requirement is symbolically represented as:

Horse

The cutaneous muscle in horse is thin and incompletely developed in the superficial fascia. The cutaneous muscle of the facial region (cutaneous fasciei) is incomplete and poorly developed over the mandibular space and the massetor muscle. Rostrally it passes to the angle of the mouth and blends with the orbicularis oris. The cutaneous muscle of the cervical region (cutaneous colli) is situated along the ventral region of the neck.

The frontalis muscle is absent.

The orbicularis oris forms incomplete ring around the rima oris. The levator nasolabialis is less extensive and is divided into the superior and inferior branches. Between the two branches pass the dilator naris lateralis and levator labii superioris. The levator labii superioris extends from malar, lacrimal and the maxillary bones to the upper lip in a common tendon with the muscle of the opposite side. The zygomaticus is thin, short and weak. It arises from the fascia covering the massetor. The incisivus maxillaris muscle which is absent in ox exists in horse and it lies under the mucous membrane of the upper lip. It originates on the alveolar border of the incisive bone from the level of lateral incisor to the first premolar and it inserts to the upper lip. Its action is to depress the upper lip. The incisivus mandibularis which is also absent in ox exists in horse under the mucous membrane of the lower lip. It originates at the alveolar border of the body of the mandible from the level of lateral incisor to the first premolar and is inserted on the skin of the lower lip and the chin. Its action is to raise the lower lip. The incisivus maxillaris and the incisivus mandibularis muscles acting together cause the closing of rima oris. The depressor labii inferioris is thicker and extends further back. The buccinator is almost similar to that of ox.

Before beginning the actual work on the establishment of a Fruit and Vegetable Processing Industry the following points should be considered:

Availability and possibilities of obtaining Funds: Investment of own money or obtaining loans from banks etc.

The scale of industry (Large, Small, Cottage or Home): Has to be decided based on availability of raw material, funds availability, land, labour and other resources.

The location: The processing unit should be located near to the actual production site of raw material so that the transportation costs of high bulk raw materials is reduced. There is no fun or justification of establishing apple processing industry in Lucknow or Bangalore and mango processing industry in Shimla as the related raw materials would have to be transported through long distances.

Site: Site selected in the location should be sunny area free from dampness and sufficient space for waste disposal should be ensured.

When rocks and minerals under go weathering process large quantities of soluble salts are formed. In humid regions these salts are washed down to the ground water and to the sea. But in arid and semi arid regions they accumulate in the soil. Excessive irrigation and poor water management are the two chief causes of water logging and salt accumulation. An accumulation of salts in soil leads to unfavourable soil water-air relationship and effect the crop production.

The following are the main causes which leads to development of salty soils (salinity or alkalinity)

Ridge gourd also known as ribbed gourd or angled gourd or silky gourd or angled loofah or vegetable gourd (Luffa acutangula (Roxb. L., 2n = 2x, family Cucurbitaceae, mostly monoecious and cross-pollinated but behaving like self-pollinated crop in term of no inbreeding depression) is not a crop of global importance; rather it is grown and consumed primarily in India and the adjoining countries which used to be part of greater India. In India, estimated area is 50,000 ha spread over almost entire country but the leading states are UP, Bihar, West Bengal, MP, Chhattisgarh, and Maharashtra, Karnataka, Tamil Nadu and north east hill region.

Origin and Domestication

Ridge gourd has similar origin as that of sponge gourd (refer to page 428).

The lymphatics of the head and neck region is comprised of the lymph nodes and the lymph ducts. The lymph nodes of the head are the parotid, pterygoid, mandibular, the lateral and medial retropharyngeal, and the rostral and caudal hyoid. The lymph nodes in the cervical region are the superficial and accessory superficial, cranial, middle and caudal deep cervical and the costocervical and subrhomboidal lymph nodes. The lymph from these lymph nodes is finally collected and carried to the common jugular vein by the tracheal and right lymphatic ducts.

Lymph nodes of head

Parotid lymph node

The parotid lymph node is large and elongated gland usually single but sometimes two small glands connected closely with each other by a fibrous tissue lying on the upper part of the lateral surface in the caudal half of the masseter muscle. It is ventral to the temporomandibular joint, partly or completely covered by the dorsal part of the parotid salivary gland and is situated lateral to the maxillary vessels and nerve. The afferent lymph vessels are received to this gland (node) from the muscles of head, ocular and auricular muscles, the parotid salivary gland, the gums, lips, appendages of the eye, ear, lacrimal gland, and from the bones of the head such as frontal, malar, nasal, maxilla, incisive, turbinate, mandible etc. The afferent from the parotid lymph node joins the tracheal duct of the corresponding side.

Herbal galactagogues are medicinal plants used to stimulate, sustain, and boost milk production. Several plants known for their galactagogue activity are being used traditionally in many cultures to stimulate milk production in animals. However, there is a knowledge gap regarding the safety and efficacy of these medicinal plants. Moreover, the mechanism of action of the herbal constituents present in these plants is not yet elucidated. Although, many herbal galactagogues have been reported as safe in domestic animals and can be used as food supplements in the dairy industry. Nowadays, the use of plantderived supplements has increased in the dairy sector promoting a new trend of organic dairy farming. Traditional remedies being used for increasing milk production in animals often incorporate herbal treatments, mainly comprising
of the following important herbs [1-8]:

8.1. Origin and distribution

• Native land is Central Asia, Middle Asia and Northeastern Iran.

• China produces 80% of the world’s supply

• India, Korea, Spain, Egypt, USA, Thailand and other countries are growing garlic.

• In India, cultivated in Gujarat, Haryana, Madhya Pradesh, Odisha, Maharashtra, Uttar Pradesh and Rajasthan

• India exports fresh and chilled garlic, dried garlic, dehydrated garlic flakes, dehydrated garlic powder, and garlic oil to Bahrain, Bangladesh, Germany, Japan.

8.2. Economic importance

• It contains amino acid allicin.

• Used both as a food flavoring and as a traditional medicine

• Allicin, antibacterial substance, the principle of amoebic dysentery.

• The garlic odour is due to diallyl disulfide.

There are eight pairs of cervical spinal nerves in ox. The first of these emerges out through the lateral vertebral (alar) foramen of the atlas vertebra. The second through the lateral vertebral foramen (at the cranial border of the arch) of the axis vertebra and the eighth nerve emerges through the intervertebral foramen between the last cervical and first thoracic vertebra. The third to seventh nerves emerge through the corresponding intervertebral foramen. Each of these nerves divides into dorsal and ventral branch immediately after their exit from the vertebral canal through respective foramina. The dorsal branches are larger than the ventral except for the first and last two and most of them divide in to medial and lateral branches. They supply the dorsal (dorsolateral) cervical muscles, skin and the cervical part of the nuchal ligament. The ventral branches are distributed to the ventral cervical muscles and skin, The ventral branches of the last two cervical nerves are large.

This method has been first described by H. H. Lowe in 1927. This is the most widely used method to handle segregating generations following crosses between two parents to develop new cultivars combining desirable traits from both the parents. In this method, superior types are selected in successive segregating generations and a record is maintained of all parent-progeny relationships. The records help the breeder to advance only progeny lines with plants that exhibit genes for the desired traits. Selection begins in F2 generation where individual plants are selected which in judgment of breeder will produce the best progeny in F5/F6 when the selection is finally concluded. In F3 and F4 generations, many loci will have become homozygous and family characteristics begin to appear. By F5/F6 generation, most families are expected to be homozygous and uniform and hence selection within such families is no longer effective. At this stage selection emphasis shifts to the selection among families rather than within families.

Selection of parents is of great significance for the success of this method. Normally, this method is used to replace already established variety. Therefore, one parent should be selected on the basis of proven performance in the particular area where the breeding is in progress. The second parent should be such that it complements the first parent for the character under improvement. Sometimes, three or four parents may also be used to supply the desired character by way of three-way or double crosses.

Metabolic profile testing (MPT) refers to the use of a battery of tests for the diagnosis of subclinical nutritional and metabolic disease in dairy cows on a herd basis. Typically, blood samples from 5-7 animals per production group are tested for various analytes. MPT is most useful in the diagnosis and management of periparturient disease. Specific nutrient imbalances, either deficiency or excess, in the diet of late-gestation cows have been related to increased prevalence of milk fever, hypomagnesemia, retained placentas, downer cow syndrome, mastitis, udder edema, ketosis, hepatic lipidosis, and displaced abomasums.