eChapters

Introduction

Chestnut (Castanea sativa Mill.) belongs to the family Fagaceae and is a deciduous tree. It is also known as sweet chestnut and has a chromosome number of 2n=24. Chestnuts have been used for centuries for various purposes such as food, timber, and medicine. They are an important source of carbohydrates and contain several nutrients such as vitamins, minerals, and fiber (Laribi et al., 2020). In India, chestnuts are mainly grown in the hilly regions of Jammu and Kashmir, Himachal Pradesh, and Uttarakhand.

Origin and distribution

Chestnut is believed to have originated in the Mediterranean region and was later introduced to other parts of the world. It is widely grown in Europe, Asia, and North America. In India, chestnuts were introduced by the British in the 19th century and were mainly grown as an ornamental tree. It was only after Independence that chestnuts were recognized as a potential crop for the hilly regions of the country (Bhat et al., 2016).

Area and production

The total area under chestnut cultivation in India is estimated to be around 1000 hectares, with an annual production of around 1000 metric tons (Bhat et al., 2016). The major states growing chestnuts are Jammu and Kashmir, Himachal Pradesh, and Uttarakhand.

Introduction

Cranberry (Vaccinium macrocarpon Aiton) belongs to the Ericaceae family and is native to North America. Cranberries are also known by various other names, including American cranberry, bearberry, and lingonberry. The plant has a diploid chromosome number of 24 and grows in acidic, nutrient-poor soils in wetlands and bogs. It is an important commercial crop worldwide due to its high nutritional value, antioxidant properties, and health benefits. Cranberries are primarily consumed as juice, dried fruit, or in the form of sauces and jellies. They are a rich source of vitamins C and E, dietary fiber, anthocyanins and essential minerals such as potassium, manganese, and copper. The fruit is also used in the pharmaceutical industries due to its anti inflammatory, antimicrobial, and anti aging properties (Santos et al., 2017; Wang et al., 2017).

The composition of cranberries is quite remarkable, with high levels of antioxidants, including phenolic compounds such as flavonols, anthocyanins, and proanthocyanidins. These compounds are responsible for the fruit’s vibrant red color and are thought to provide many of the health benefits associated with cranberries. Cranberries are an important crop in the United States, Canada, and Chile, with significant economic value. They are primarily consumed as juice or in processed forms, such as sauces and jams. Cranberries are also used in the production of dietary supplements and cosmetics.

1.0 Introduction

Kiwi or kiwifruit (Actinidia deliciosa) belongs to the Actinidiaceae family that is a fruiting vine originated from Yangtze Valley of China. Kiwifruit was named because it resembles a flightless bird, kiwi which is New Zealand’s national symbol. It is also referred as “New Zealand’s horticultural wonder”. In china kiwi was named as Yang Tao and also as “China’s miracle fruit”. Over the past century, kiwis have also acquired a variety of strange names, including “melonette”, “wood berry”, “strange fruit”, “monkey peach, “macaque pear”, “mihoutau”, “Chinese gooseberry” (Tyagi et al., 2015) “vine pear”, “sun peach” and the particularly enticing moniker “hairy bush fruit” (Hugh Cambbell and Julia Haggerty., 2008). Kiwifruit is a nutrient dense fruit which is exceptionally known for its flavour and vitamin C content (Guroo et al., 2017). Digestive potential, antioxidant property, healthy metabolism, immune function and protection from coronary artery disease are the therapeutic benefits of kiwi (Feruguson., 2003; Boeing et al., 2012; Singeltary., 2012). It reduces the risk against cancer (Puri and Manjula., 2018), arteriosclerosis, cardiovascular diseases and irritable bowel syndrome (Chang et al., 2012) as it is a rich source of ascorbic acid and polyphenols. Due to palatability, aromatic compounds present in kiwifruit attract many consumers. Unique flavour and aroma is increased due to the presence of aldehyde E-2-hexenal, methyl benzoate, ethyl butanoate and esters. China tops in kiwifruit production followed by Italy. Two biggest exporters of Kiwi in world are Italy and New Zealand (Testolin & Ferguson, 2009). Kiwifruit has excellent pharmacological profile and it helps in development of nutritional supplements for welfare of mankind. Farmers can earn high return per unit area as it bears heavily every year and has no crop failure. Thus, kiwifruit has bright prospects in our country.

Introduction

The olive fruit, scientifically known as Olea europaea L., is the edible fruit of the olive tree. It belongs to the Oleaceae family, which encompasses various other plants such as jasmine and lilac (Bartolini & Prevost, 2002). The scientific name Olea europaea reflects its European origin, with the genus name, Olea, derived from the Latin word “oleum” meaning oil, emphasizing the fruit’s high oil content. The species name, europaea, signifies its prevalence in Europe (Vossen, 2007). In India, the olive fruit is commonly referred to as “Jaitoon” or “Jaitooni” (Bhardwaj & Gupta, 2019). These names are derived from the Hindi language and are used to denote both the tree and its fruit. The olive tree has a diploid chromosome number of 2n = 46 (Besnard et al., 2008). This indicates that each cell of the olive tree contains 46 chromosomes, arranged in 23 pairs. Understanding the chromosome number is essential for genetic studies and breeding programs as it helps in comprehending the genetic diversity and variability of the olive fruit.

Importance

The production of olive fruit in India has gained significant importance in recent years due to various factors. Firstly, olive fruit is a valuable source of edible oil. Olive oil is renowned for its health benefits, being rich in monounsaturated fats, antioxidants, and vitamins (Vossen, 2007). The cultivation of olive fruit provides economic opportunities for farmers and contributes to the country’s agricultural diversification.

Introduction

Pear (Pyrus communis L.) is one of the most important temperate fruit crops after apple in the family Rosaceae. It is diploid and a member of the Pomoideae subfamily, having chromosome number 2n=2X=34. The pear is a tree and fruit in the Rosaceae family, which also includes apples and quince. The pear has a fibrous center and is a mildly sweet fruit. It is abundant in dietary fiber, plant components, and vital antioxidants. Pears come in a variety of shapes, including round, oval, and elongated, and they can be smooth or bumpy in texture. The fruit has a thin skin that can be smooth or rough, and it ranges in color from green to yellow to red. The flesh of the pear is white, yellow, or cream-colored and is juicy and sweet, with a slightly gritty texture. Later in the 19th century, better pear cultivars were introduced in India. Pears come in second place among temperate fruits in India in terms of both production and land area. Due to the pear’s extensive climatic and soil tolerance, it is grown in both temperate and subtropical climate.

Importance and composition and uses

Pears are a good source of fiber and vitamin C, and they are relatively low in calories. Pear fruits are suggested for diabetic patients because of their low sugar content, which also lowers blood pressure and regulates heart rate. The 3.1 gm of dietary fibre it contains are very beneficial for intestinal health. About 27 to 41 mg of phenolics are present per 100 mg of fruit, making it a strong source of antioxidants. Moreover, pears have demonstrated vasodilator capabilities, and their components anti-arrhythmic effects often lower blood pressure and regulate heart rate. Fruits include the folic acid that makes up the vitamin B complex. It contains the glycoside arbutin, which has potential as a treatment for urinary tract infections. The body’s uric acid is helped to leave the body with its help. The pears’ leaves are effective in treating swelling and blisters. Fever, anaemia, and general malaise are also treated with their assistance. A destion can be made from the bark of pears. 

Importance and uses

Walnut is a valuable nut crop that is grown in several regions of the world, including India. The nut is a rich source of several nutrients, including protein, fiber, omega-3 fatty acids, and antioxidants. Walnuts have several health benefits, including reducing the risk of heart disease, improving brain function, and reducing inflammation. They are also used in several industries, including food, pharmaceuticals, and cosmetics.

Walnuts have been an important food source for humans for thousands of years. In ancient times, they were believed to have medicinal properties and were used to treat several ailments (Martinez et al., 2010). Today, they are used in the food industry to produce confectionery, bakery products, and as an ingredient in several cuisines. They are also used in the pharmaceutical and cosmetic industries due to their anti inflammatory and anti-aging properties (Alasalvar et al., 2010; Bolling et al., 2011).

Walnuts are a rich source of antioxidants, which are important for maintaining good health. Antioxidants help to neutralize free radicals in the body, which can cause cell damage and lead to several chronic diseases, including cancer and heart disease (Alasalvar et al., 2010). In addition, walnuts are an excellent source of omega-3 fatty acids, which are important for brain health and reducing inflammation in the body (Bolling et al., 2011). Walnuts also contain fiber, which is important for maintaining healthy digestion and reducing the risk of colon cancer (Martinez et al., 2010).

Introduction

The pistachio nut (Pistacia vera L.), a small tree native to Afghanistan, Central Asia, and Iran. The Pistacia genus is a member of the Anacardiaceae family, which also includes the cashew, mango, sumac, and poison ivy plants. The tree yields seeds, which are frequently eaten as food. The genus Pistacia has about 20 species including Pistacia vera, Pistacia atlantica, Pistacia terebinthus, Pistacia khinjuk, and Pistacia lentiscus (Parizi et al., 2016). Western Asia is the pistachio nut home region, and traders brought them to the Middle East, the Mediterranean nations, and Europe. Pistacia vera L. is the only species in this genus commercially grown for edible nuts. It was probably first grown in areas close to where it grew wild, as remains of pistachio nuts dating to the 6 millennium BC have been discovered in both Afghanistan and south-eastern Iran in recent times. Pistachio cultivation progressively spread to the West from the old Persian Empire, where it was widely grown. the word “Pistachio” etymology most likely stems from the Avestan word “pistak” from ancient Persian (Mandalari et al.2022).

Health benefits

In pistachio nuts more than 50% of the fat is unsaturated. There are also 20% proteins, 20% complex carbs, and pro-vitamin A. Dried fruit pistachios nut has the highest potassium content that is very good for health. It is also great supplier of energy, magnesium, phosphorus, and iron. The heart’s functioning can benefit from the consumption of this fruit. Moreover, it participates in cell metabolism, and the potassium it contains controls the water content of the cells. The production of hemoglobin and myoglobin depends on iron, consumption of pistachio nuts fulfill the iron requirements. It contributes to cellular respiration, intellectual growth, and immune system amplification. A crucial component of bones and teeth is phosphorus. Moreover, magnesium aids in the formation of bones and the production of proteins. This nut is good source of phosphorus and magnesium also.

Introduction

Plum, scientifically known as Prunus domestica, is a popular fruit-bearing tree belonging to the Rosaceae family. Renowned for its delicious and versatile fruits, plum cultivation has a long history that spans different cultures and regions. Plums are enjoyed worldwide both in their fresh form and processed into various culinary delights. Because of its temperate character, plum is a significant crop in hills, but it is just a modest fruit crop in Punjab and the north Indian plains. Because it requires little freezing and has a shorter juvenile phase, it is ideal for planting as a filler fruit plant in mango, litchi, and pear orchards. There are a few solid-block plum plantations in the Amritsar, Fazilka, Ambala, and Kurukshetra regions as well. Due to the trees’ abundant f lowering, high fruit set, and early ripening habits of varieties suitable for plains, they are extremely productive. A fully developed tree can typically produce 50–60 kg of fruit. Plum trees are deciduous and typically bear fruits during the summer season. The fruits come in a wide range of colors, including shades of purple, red, yellow, and green, with variations in size and flavor. This diversity in plum varieties allows for a delightful selection for consumers, catering to their individual preferences.

Introduction

Raspberry, scientifically known as Rubus idaeus L., belongs to the family Rosaceae. It is also known as “red raspberry” or “European raspberry,” and its chromosome number is 2n=14. Raspberry is an important and popular fruit crop due to its rich flavor, high nutritional value, and various medicinal properties. Raspberry is rich in vitamins, minerals, dietary fiber, and antioxidants, making it a valuable component of a healthy diet (Rahman et al., 2020). Raspberry is widely used in the food industry for jam, jelly, juice, ice cream, and other processed products.

Origin and Distribution

Raspberry is believed to have originated in Eastern Europe and Western Asia (Farrar, 2016). Raspberry was introduced to India during the British era and was initially cultivated on a small scale in the hilly regions of the country. Today, raspberry cultivation has spread to various parts of India, including Himachal Pradesh, Jammu and Kashmir, Uttarakhand, and Sikkim. The major raspberry-growing states in India are Himachal Pradesh and Jammu and Kashmir.

Introduction

Strawberry (Fragaria x ananassa Duch.) is a perennial herb that belongs to the Rosaceae family (Bayat et al.2019). Strawberry is sweet and has a distinct fragrance. Strawberries are a popular fruit that are known for their sweet taste, bright red color, and high nutritional value. It is abundant in several vitamins and nutrients, including carotene and anthocyanins. It is popularly regarded as the important table fruit of millions of people around the globe and well-liked by consumers. Strawberry, which has 98% edible component, is regarded as a whole fruit. typically eaten fresh and raw. They are tasty, healthful, and low in calories. Strawberries grow well in India in conditions that are often temperate in the north, subtropical in the plains, or tropical at high altitudes. The primary reason strawberry production is still in its infancy in India, despite its handsome fruits and palatable flavour, is that it is a perishable crop (Pramanick et al. 2012). The strawberry is a fruit that grows all over the world. The Roman poets Virgil and Ovid wrote about it in the first century, and gardeners have been growing strawberries in England since the sixteenth century (Boriss et al. 2006).

Cape gooseberry (Physalis peruviana L.)

Cape gooseberry, scientifically known as Physalis peruviana L. (Physalis edulis Sims) belongs to family Solanaceae. Fruits are enclosed inside a bladder- like husk which turns papery when mature making all seeds inside visible. There are more than 70 species under the genus Physalis. Out of these species only few of them are of economic value. Cape gooseberry is most widely known among these species as it bears superior fruit in comparison to other species. The fruits are good in taste and a good source of minerals and vitamin such as vitamin B complex, vitamin C and pro-vitamin A, rich in pectin.

Species diversity

Cape goosberry is also known by different names in Latin America: capuli, aguaymanto, tomate sylvestre, or uchuba, in Bolivia; uvillain, in Peru; capuli or motojobobo embolsado, in Colombia; topotopo, chuchuva, in Ecuador; uchuva, vejigón or guchavo in Venezuela; capuli, amor en bolsa, or bolsa de amor, in Chile; cereza del Peru in Mexico; teparee, tiparee, makowi, etc., in India. Physalis pruinose L. commonly known as strawberry tomato, husk tomato or ground cherry is also grown for its small yellow fruits. In mild-temperature climates, the fruit is used to make sauce, pies, and preserves. Unripe druits are toxic. In Australia, the plant is thought to have caused illness and death in cattle.

Introduction

Walnut (Juglans regia L.) is an important temperate nut crop grown in various parts of the world. It belongs to the family Juglandaceae and is commonly known as English walnut, Persian walnut, or common walnut. It has a chromosome number of 2n=32 (Kalia et al., 2016). Walnuts are a rich source of omega-3 fatty acids (Husain et al., 2013). The walnut fruit is a drupe that contains a nut surrounded by a hard shell. The nut is enclosed in a fleshy green or brownish-yellow hull, which splits open when the fruit is ripe. Walnut is an important nut crop due to its high nutritional value and medicinal properties. The kernel of the walnut is rich in protein, fat, and minerals such as calcium, phosphorus, and magnesium. It also contains vitamins B1, B2, and E. In addition to being used as food, walnut has been used in traditional medicine for the treatment of various ailments such as diabetes, cardiovascular diseases, and cancer (Liu et al., 2017). Walnut oil is rich in linoleic acid and is used in the production of cosmetics and soap. The leaves and bark of the tree are used in traditional medicine to treat various ailments such as diabetes, fever, and inflammation.

Abstract
Aquatic sustainability is vital for maintaining the health and productivity of aquatic ecosystems. Human activities have significantly impacted these environments, leading to pollution, habitat destruction, and biodiversity loss. Green technologies offer innovative solutions to address these challenges. Key components of aquatic sustainability include biodiversity, water quality, ecosystem health, resource management, and social equity. Aquatic sustainability is crucial for food security, economic benefits, climate regulation, cultural and recreational values, and ecosystem services. Current challenges facing aquatic ecosystems include pollution, overfishing, habitat destruction, climate change, invasive species, plastic pollution, and habitat degradation. Green technologies play a crucial role in addressing
these challenges by reducing pollution, promoting sustainable fisheries management, mitigating climate change, restoring habitats, conserving water, and monitoring aquatic ecosystems. Green technologies such as advanced water filtration systems, nanotechnology, bio-inspired filters, desalination technologies, UV-C light, ozone, eco-friendly wastewater management, integrated constructed wetlands, biological treatment systems, remediation technologies, natural infrastructure solutions, and biodegradable materials offer sustainable and effective solutions for aquatic sustainability.
Keywords: Aquatic sustainability, Pollution, Green energy, Nanotechnology

1. Introduction:- Understanding Good Laboratory Practices (GLP)
1.1 Definition and Core Principles of GLP
The Organisation for Economic Co-operation and Development (OECD) defines Good Laboratory Practice as “a managerial quality control system that governs the organizational process and the conditions under which non-clinical health and thus, environmental safety studies are well planned, executed/ performed, vigilantly monitored, properly recorded, reported, and finally archived”. This definition highlights that Good Laboratory Practices (GLP) is a comprehensive framework focused on ensuring the quality and integrity of the entire study lifecycle, rather than dictating the scientific validity of the research itself. The primary goal of GLP is to ensure process integrity and data traceability, which are essential for the regulatory acceptance of safety data for a variety of products. These products include pharmaceuticals, pesticides, cosmetics, veterinary drugs, food additives, feed additives, and industrial chemicals, all of which undergo non-clinical testing to assess their potential health and environmental risks. It is important to note that GLP principles specifically apply to these non-clinical studies and exclude research involving human subjects. The OECD Principles of GLP are internationally recognized and serve as the basis for GLP regulations in numerous countries worldwide. These principles outline the responsibilities of key personnel involved in non-clinical studies, including test facility management, the study director, study personnel, and quality assurance personnel. Furthermore, they establish minimum standards concerning the suitability of facilities and equipment to perform studies, as well as the necessity for comprehensive Standard Operating Procedures (SOPs) to guide all aspects of laboratory operations. The core principles of GLP encompass a wide range of elements critical to ensuring the quality and integrity of laboratory studies. These OECD principles of Good Laboratory Practices (GLP) include: the proper organization team and personnel staff members within the testing facility, the well establishment of a robust quality assurance programme, the better suitability and proper maintenance of various necessary facilities, the efficient management of apparatus, materials, and reagents, the characterization and control of test systems, the handling of test and reference items, the development and adherence to Standard Operating

Contemporary tilapia cultivation is aligned with the principles of circular bioeconomy, aiming to minimize resource inputs while maximizing waste and effluent reuse. This strategy involves closing the loops of various economic and ecological resources as well as decentralizing the production systems for localized production and consumption. Growing concerns about disease outbreaks along with market demands for environmentally responsible and sustainable aquaculture practices are propelling a transformative shift. This shift is characterized by structural advancements in water and effluent reuse through closed-loop recirculation systems that repurpose waste as valuable nutrients. Today one of the pivotal trends in tilapia farming is the adoption of circular bio-economy principles which is exemplified by diverse recirculation systems including Biofloc Technology (BFT), Partitioned Aquaculture Systems (PASs), Recirculatory Aquaculture Systems (RAS), bio RAS, , In Pond Recirculation Systems (IPRS) and Integrated Multitrophic Aquaculture (IMTA). The trajectory of tilapia culture often aligns with the trajectory of urban agriculture as well as waste fermentation. Low-demand water recirculation systems are poised to spearhead a disruption in industries across five key sectors (material, information, energy, transport, and food/ health) that rely heavily on extraction. This transformation is driving the transition towards a more sustainable and localized model.

The age-old struggle between crops and diseases has been a perpetual challenge for farmers and agricultural stakeholders worldwide. Weather patterns play a pivotal role in the development and spread of crop diseases, making it essential to integrate weather wisdom into crop disease management strategies. By analyzing historical and real-time weather data, farmers and agricultural experts can anticipate, prepare for, and respond to disease outbreaks more effectively, minimizing crop losses and ensuring food security. Effective crop disease management based on weather wisdom involves a three-pronged approach. It begins with caution, where historical climate data and disease surveillance reports are analyzed to identify potential correlations between weather patterns and disease outbreaks. This information helps farmers and agricultural experts to recognize early warning signs of disease susceptibility, such as temperature fluctuations, precipitation patterns, and humidity levels. For instance, excessive rainfall can lead to an increased risk of fungal diseases, while drought conditions can exacerbate bacterial diseases (Fig. 13.1). The confirmation phase involves closely monitoring real-time weather forecasts and disease surveillance data to detect early warning signs of an impending disease outbreak. Advanced technologies, such as satellite imaging and sensor-based monitoring systems, provide valuable insights into crop health and disease development. By combining these data streams, farmers and agricultural experts can confirm the presence of disease-causing pathogens and take targeted action to prevent their spread. Finally, a call to action is triggered, prompting farmers and agricultural stakeholders to implement integrated disease management strategies. These may include cultural practices, such as crop rotation and sanitation, biological controls, such as introducing beneficial microorganisms, and chemical controls, such as targeted fungicide applications. By leveraging weather wisdom, farmers can optimize their disease management strategies, reducing the reliance on chemical pesticides and minimizing environmental impact. By embracing weather wisdom, crop disease management can be transformed from a reactive to a proactive approach. Farmers and agricultural stakeholders can stay one step ahead of disease-causing pathogens, protecting their crops and ensuring a stable food supply. As we explore the intersection of weather wisdom and crop disease management, it becomes clear that this integrated approach holds tremendous potential for revolutionizing the way we predict, prevent, and respond to crop diseases.
13.1 Caution
Approaches to Caution Farmers on Crop Diseases, Information Generation, and Communication using Mobile Technology To caution farmers on crop diseases, it is essential to adopt a multi-faceted approach that leverages mobile technology to generate and disseminate critical information. This approach should focus on empowering farmers with timely and accurate information, enabling them to take proactive measures to prevent and manage crop diseases. Firstly, a robust disease surveillance system should be established to monitor and track crop disease outbreaks in real-time. This can be achieved through a network of field scouts, extension agents, and research institutions that report disease incidence and severity to a central database. Mobile-based applications can be used to facilitate data collection, transmission, and analysis, ensuring that critical information is captured and disseminated promptly. Once disease outbreaks are detected, mobile technology can be harnessed to generate and disseminate alerts to farmers. This can be achieved through Short Message Service (SMS) or voice messages, which can be sent to farmers’ mobile phones, warning them of potential disease risks and providing advice on management strategies. Mobile-based applications can also be used to push disease alerts, advisories, and management recommendations to farmers, ensuring that they receive critical information in a timely and accessible manner. To enhance the effectiveness of these alerts, it is essential to ensure that they are tailored to the specific needs and contexts of farmers. This can be achieved

The effects of weeds, pests, and diseases result in the loss of between 20 40% of the world’s potential agricultural yield each year. By reducing crop loss from pest infestations, the use of pesticides can boost agricultural output by 25–50%. Only 25 to 30 percent of the area that is now being grown is protected by pesticides. Because domestic pesticide use is among the lowest in the world, there is significant room for expansion. India uses 0.6 kilogramme of pesticides per hectare compared to the global average of 3 kg per hectare. The use of crop protection products must be enhanced. There are now 1175 compounds accessible in the world, however only roughly 292 of them are registered in India.

Advantages of Using Pesticides

Pest are an ecological problem and therefore our control strategy must be ecologically sound. Modern agriculture is a combination of both and human intervention is necessary, whether it be pulling out weeds by hand, use of pesticides or genetic engineering. Control methods evolve over time as knowledge and techniques improve. This includes the development of chemical means of control which become very important because of a number of advantages

Pest are an ecological problem and therefore our control strategy must be ecologically sound. Modern agriculture is a combination of both and human intervention is necessary, whether it be pulling out weeds by hand, use of pesticides or genetic engineering. Control methods evolve over time as knowledge and techniques improve. This includes the development of chemical means of control which become very important because of a number of advantages

During recent time, world agriculture is in search of sustainability in farming. However, it is tedious job for the developing countries because of poor input management and degradation of resources, ever-growing human population and increasing demand for food and livestock feed, and shrinkage of land and other natural resources.

ABSTRACT

Due to the exorbitant price of the popular gelling agent agar, Plant Tissue Culture (PTC) has gradually become a costly technique in recent times. So, alternative sources of agar needs to be popularized which at one hand should be able to meet all the positive properties of agar and on the other hand be cost effective as well. The seed husk obtained from Plantago ovata Forsk. is such an alternative which was successfully used as a gelling agent for media preparation in PTC experiments for in-vitro shoot and root development in Nicotiana tabacum L. and Ocimum gratissimum L. The nodal explants were grown in MS basal medium (MSo) supplemented with 3% sucrose and were gelled with progressive concentrations of isabgol husk to ascertain the optimum concentration for better survival and growth response, while 0.8% agar acted as a negative control. The response of the explants on media gelled with husk was compared to that of media gelled with 0.8% agar. It was inferred that 0.3% husk in the medium was optimum for best growth response which was comparable to that of agar. Consequently, the estimated cost of a liter of MSo reduced by almost Rs.61.15/- (compared to Himedia’s Agar) to Rs.177.57/- (compared to Sigma’s Agar) after using isabgol seed husk as a gelling agent in media preparation.