eChapters

Bulk population method of breeding is defined as the growing of genetically diverse populations of self-pollinated crops in a bulk plot with or without mass selection, followed by single plant selection. It is applicable to self-pollinated crops and not applied to cross-pollinated crops. Bulk population breeding is a strategy of crop improvement in which natural selection effect is solicited more directly in the early generations of the procedure by delaying stringent artificial selection until later generations. The Swede, H. Nilsson-Ehle, developed the procedure. H.V Harlan and colleagues provided additional theoretical foundation for this method through their work in barley breeding in 1940s. As proposed by Harlan and colleagues, the bulk method entails yield testing of the F2 bulk progenies from crosses and discarding whole crosses based on yield performance. In other words, the primary objective is to stratify crosses for selection of parents based on yield values. The current application of the bulk method has a different objective.

The first step in applying the correct rate of fertilizer is calculating crop nutrient requirements. A soil test is the only way to measure how much P₂ O₅ and K₂ O are available in soils, and soil tests are available through several private and public laboratories.

Fertilizer Calculation

To compute the equivalent amount of fertilizer necessary to supply the corresponding amounts of nutrients, the following formula is used:

NTSYSpc 2.0 software is used for various types of analysis including calculation of similarity index values and construction of dendrogram. Construction of dendrogram using gel data in the form of bands. This software accepts zero-one data.

Modes of Culture 

Callus is a mass of undifferentiated plant tissues formed from plant cells or tissue cuttings when grown in culture. Tissues and cells of plant cultured in a liquid medium aerated by agitation grow as suspension of single cells and cell clumps. For growth, the cells require to divide, whereas, the cells breaking up from explant are mature, nondividing. Hence, the differentiated tissue undergoes modifications to become meristematic. This phenomenon of a mature cell reverting back to meristematic state to form undifferentiated callus tissue is called dedifferentiation.

Canopy management is central to achieving balanced vegetative growth, efficient light utilization, flowering, fruiting, and high-quality produce. This chapter explains the principles and objectives of training and pruning in horticultural crops. Different training systems are related to crop architecture, growth habit, planting density, and production objectives. Pruning techniques are discussed for regulating shoot growth, removing undesirable branches, improving air circulation, and maintaining productive canopy structure. Light interception and distribution within the canopy are also examined because photosynthetic efficiency strongly influences yield and fruit quality. Modern high-density orchards require precise canopy management to balance productivity, plant size, mechanization, and long-term orchard sustainability.

10.1 Introduction

A single-walled carbon nanotube (SW-CNT) is a nano scale tube formed by a cylindrical shell of single atomic layer of carbon atoms. Nanotubes have diameter of a few nm and length up to 100 nm so that they form extremely thin wires. The atomic structure of SWCNT can be formed by wrapping a stripe of single atomic layer of graphite sheet al.,ong a certain direction, and this direction determines the diameter and chirality of the nanotubes. Experimental and theoretical studies have found that these nano-meter sized CNTs have novel electronic properties, which can be metallic or semiconducting, depending on their radius or chiralities (Mintmire 1992, Hamada 1992, Saito et. al., 1992, Rao 1997, Wildoer 1998 and Odom et al., 1998).

Carnation is one of the world's most important commercial cut flowers and is extensively produced under protected conditions for domestic and export markets. This chapter examines its origin, distribution, genetics, botanical characteristics, types and commercially important varieties. It describes protected cultivation, greenhouse design, climate management, soil, propagation, planting, irrigation, fertigation, pinching, disbudding, staking and growth regulation. Major pests and diseases, harvesting, grading, postharvest handling, vase life and yield are also discussed. The chapter incorporates modern cultivars, disease-resistant varieties and genetically modified colour developments, making it valuable for contemporary commercial carnation production and floriculture education

The Cartagena Protocol on Biosafety entered into force on 11 September 2003. It is a legally binding international agreement under the United Nations’ Convention on Biological Diversity (CBD).

What is a protocol ?: A protocol is a binding international instrument, separate from, but related to, another treaty. It is a separate instrument, that must be individually negotiated, signed and eventually ratified. It is only binding on States that become parties to it. It thus has its own parties, and creates separate rights and obligations for them, as any other treaty.

The unique characteristic of a protocol is that it is related to a ‘parent’ treaty, through substantive, procedural, and institutional links. Most importantly, a protocol under a specific treaty must comply with the parent treaty’s provisions authorizing and regulating the adoption of protocols under its auspices. 

6.1 Introduction
The success of design thinking depends on its applicability in industries and service organization. The applications of design thinking applied to product development should focus on the needs of users. The design thinking can also be applied to design new processes as well as improve an existing process.
6.2 Case Studies
The section presents several case studies of applying design thinking in realities as listed below.
1. Design thinking in eye care
2. Design thinking in foundry
3. Design thinking in FMG company
4. Design thinking in auto industry
5. Design thinking at retail outlet
6. Design Thinking in Flight Reservation System
7. Design thinking in winding fabric on roller in textile mill
6.2.1 Case study: Design Thinking in Eye Care
Eye care is a crucial part of health care system. There are many factors that are responsible for poor eye vision. Normally in the early stages, viz. children and adults up to 40 or 45 years of age may have eye power issues, which are managed

1. Introduction
Animal experimentation remains a cornerstone of scientific research in many fields, from medical advances to pharmaceutical testing. However, the ethical treatment of laboratory animals has become a point of significant concern worldwide. India, through the Committee for the Control and Supervision of Experiments on Animals (CCSEA), ensures that ethical standards are maintained in research institutions. Established under the Ministry of Environment, Forests, and Climate Change (MoEF&CC), CCSEA is tasked with overseeing the use of animals in experiments, ensuring that procedures adhere to ethical guidelines and international norms. CCSEA’s guidelines are in line with international standards set forth by organizations such as the International Council for Laboratory Animal Science (ICLAS) and the European Union (EU), but they are tailored to address local needs and conditions. The guidelines, which focus on the welfare of laboratory animals, emphasize the ethical review process, animal care, and the importance of minimizing animal suffering. They also stress the need for researchers to implement the 3Rs—Replacement, Reduction, and Refinement—to minimize the use of animals and ensure humane practices in research. This chapter explores the CCSEA guidelines in detail and compares them with animal welfare regulations from around the world, including the United States, European Union, Japan, and Australia. By understanding the similarities and differences between these regulations, researchers and policymakers can ensure the ethical treatment of laboratory animals on a global scale.
2. Objectives of CCSEA Guidelines
The main objective of the CCSEA guidelines is to ensure that laboratory animals are treated ethically and humanely while also supporting the validity and scientific rigor of research. The guidelines encourage researchers to prioritize animal welfare while minimizing unnecessary suffering. Specifically, CCSEA focuses on:
1. Ethical Treatment of Animals: Ensuring that animals are housed in suitable conditions, given adequate veterinary care, and provided with a comfortable environment to reduce stress.
2. Justification of Animal Use: Ensuring that animals are used only when necessary, and that their use is scientifically justified.
3. Pain and Stress Minimization: Ensuring that experimental procedures cause the least pain, distress and suffering possible.

Cell Lysis Procedure

Pellet the cells in a microfuge tube: add 1.5 ml culture, centrifuge for 15 seconds at maximum speed, discard the supernatant, and then repeat the procedure. This will result in the cells from 3 ml of culture in a single tube.

Resuspend the cells in 1 ml Lysis buffer.

Take 300 µl aliquot for Bradford protein assay.

Add 50 µl lysozyme, and mix gently.

After 10-20 minutes at room temperature, microfuge for 10 minutes to pellet cell debris.

Use supernatant for LDH assay.

Cell

Cells (small, membrane-bounded structures filled with a variety of chemicals in an aqueous solution that interact to give life) are the basic unit of life. Cells are capable of acquisition and use of energy, have the capacity to reproduce and respond to the environment and are able to carry out a variety of chemical reactions. They maintain a relatively constant internal environment despite changes in the external environment. There are two types of cells, namely, prokaryotes and eukaryotes.

The prokaryotic (pro = before, karyote = nucleus) cells are the simple form of cell types which include the various types of cells of bacteria and their relatives, all belonging to kingdom Monera. They possess a cell wall constructed of peptidoglycan, a substance unique to prokaryotes. Beneath the cell wall, there is a plasma membrane that encloses a cytoplasm containing DNA, RNA, proteins and various small molecules.

Celosia is an attractive flowering crop valued for its distinctive inflorescences, bright colours and use as loose and ornamental flowers. The crop includes forms such as Cristata, Plumosa and Spicata, with the characteristic brain-like appearance of cockscomb resulting from fasciation. This chapter deals with the origin, distribution, genetics, botanical features and important varieties of Celosia. It explains climatic and soil requirements, propagation, land preparation, nutrient management, planting, irrigation and cultural practices for commercial production. The chapter also covers plant growth regulation, insect pests and diseases, harvesting, postharvest management and yield. Its practical approach makes it relevant to students, growers and floriculture entrepreneurs.

Centre of origin are also known as centre of diversity. N. I. Vavilov in 1926 has proposed that crop plants evolved from wild species in the areas showing diversity and termed them as primary centers of origin. Primary centers, crops moved to other areas with the movements of man. But in some areas, certain crop species show considerable diversity of forms although they did not originate there. Such areas are known as secondary centers of origin of these species. Genetic diversity is essential for crop improvement. Vavilov has suggested eight main centers of origin of crop plants.

1. Chinese Centre

This centre is considered to be one of the earliest and largest independent center of origin of cultivated plants. This centre includes mountain regions of central and western China. The endemic species listed from this centre include, soya bean, radish, turnip, pear, peach, plum, colocasia, buckwheat, opium poppy, brinjal, apricots, oranges, china tea etc.

2. Himalayan Centre

This centre is also known as the Indian centre of origin. This centre includes regions of Assam, Burma, Indo-china and Malayan Archipelago. The crops originated from this centre are rice, red gram, chick pea, pea, mung, brinjal, cucumber, sugar cane, black pepper, moth bean, rice bean, cotton, turmeric, indigo, millets etc.

Crop Domestication

Domestication can be loosely defined as the process of bringing of a wild species under the management of man. Domestication is the process by which genetic changes (or shifts) in wild plants are brought about through a selection process imposed by humans. Because of the role of humans, the process results in characteristics that are beneficial to humans but some that would be disadvantageous for plants in their natural habitats. It is an evolutionary process in which selection (both natural (frost tolerance) and artificial (large attractive fruits)) operates to change plants genetically, morphologically, and physiologically. 

13.1 Challenges in the technology transfer process
Technology transfer (TT) is more complex and involves the complex interrelationships between universities, research institutes, the private sector, the state, and society. Though TT is all about translating research findings into purposeful solutions that can support the economy and society, it is not a linear process. The challenges can be described as legal, institutional, cultural, financial, policy, and human resource. All this is accompanied by bottlenecks that inhibit or decelerate the diffusion of promising technologies.
13.1.1 Legal and intellectual property challenges
The vital component of technology transfer is the proper management of intellectual property (IP), which is often the most challenging aspect.
• Ambiguities in IP ownership: During multi-institutional or shared funder research, ownership is quite controversial. As an example, when a government lab and a university collaborate on the project, there is ambiguity as to who would have the ultimate licensing authority over the technology: the university, the lab, or the funding agency. This kind of fight hinders commercialisation and scares off industry partners.
• Lengthy patent filing and prosecution timelines: In several jurisdictions, the filings, examination, and granting of a patent may take between three and five years or more. Such delay is not compatible with the rapid cycle of invention in other fields such as ICT or biotechnology, where patent claims may become invalid before they are granted.
• High costs of IP protection: International direct patenting, like under the Patent Cooperation Treaty (PCT), is costly and involves legal services, fees, and upkeep costs. These costs are excessively high, which cannot be easily compensated by universities and startups, and where technologies are not secured or limited to local markets.

Introduction
The accurate identification of crop varieties is crucial for agriculture, plant breeding, and intellectual property protection. DNA-based techniques have emerged as powerful tools for distinguishing between crop varieties due to their precision and reliability. However, several challenges persist in the adoption and implementation of these techniques. Conventionally, morphological descriptors are routinely used for establishing the identity of varieties. But these morphological descriptors suffer from many drawbacks such as influence of environment on trait expression, epistatic interactions, pleiotrophic effects etc. Furthermore, the paucity of sufficient number of these descriptors for unequivocal identification of increasing number of reference collection of varieties enforces to look for alternatives. Electrophoresis of seed proteins and isozyme analysis have overcome these limitations to some extent but now many powerful DNA based techniques are available. Since all genetic differences between individuals are laid down in the primary sequence of their genomic DNA, the straightforward method of identification of crop varieties and parental lines would be to determine this sequence for the genome under comparison. Sequencing requires huge monitory investment and it is practically impossible to sequence the whole genomes of all varieties. But with the automation in sequencing now possible, short stretches of amplified DNA can be sequenced. However, a more practical strategy would be to limit the comparisons to specific regions of the genome, which frequently differ between individuals.

Raman Spectroscopy

It is a spectroscopic technique based on inelastic scattering of monochromatic light, usually from a laser source. Inelastic scattering means that the frequency of photons in monochromatic light changes upon interaction with a sample. Photons of the laser light are absorbed by the sample and then reemitted. Frequency of the reemitted photons is shifted up or down in comparison with original monochromatic frequency, which is called the Raman effect. This shift provides information about vibrational, rotational and other low frequency transitions in molecules. Raman spectroscopy can be used to study solid, liquid and gaseous samples.

Introduction

The metal particles in nanometer scale shows exciting optical and electronic characteristics when compared with respective bulk metals. Because of the inherent electronic properties of the metal and metal oxide nanoparticles these nanostructure materials are applied in various disciplines in recent days. Mostly these nanoparticles can be synthesized either top down or bottom up approaches. The conversion of bigger size particle or matter into tiny particles is called top down approach where as smaller particles or atoms joined together forming particles in regular shape within nanometer scale dimension is called bottom up approach. The formation of nanoparticles by chemical reduction is an example for the later one and the reduction size with the help of mechanical crusing leads to the formation of fine nanoparticles is named as top down approach. Mostly nanoparticles can be synthesized by chemical method because of its easiness to synthesis particles with uniform size and high purity.

Chemical synthesis is spontaneous organization of molecules into stable, structurally well-defined aggregates at the nanometer length scale. Chemical synthesis is largely an art form (i.e., theory often does not quantitatively predict how to make new materials from molecules).Yet, chemical synthesis can be quite powerful (e.g., proteins and complex organic molecules can be made from simple reagents) Semiconductor nanocrystals have been synthesized via “natural self-assembly” in two steps:

1.Capped cluster molecule forms spontaneously from molecular reagents in solution

2.Cluster molecules crystallize out of solution