eChapters

With the current food production level of 275.11 million tonnes, India has to step up food grain production by next decade to feed the burgeoning population (Report Ministry of Agriculture). India with 2.3% of the global geographical area, supports about 16.8% human population, 20% of the world’s livestock. However, per capita availability of land for producing agricultural commodities has declined from 0.34 ha in 1951 to about 0.23 ha in 1980 and 0.15 ha in 0.15 ha during 2015 and it is expected to decline further to about 0.09 ha by 2050 A.D. With the possibility of horizontal expansion or putting more land under cultivation being remote, further augmentation in yield in Indian agriculture will be forced more and more into marginal or problematic soils accompany about 57% geographical area with increasing competition for good quality lands.

11.1 Biomass

Globally, all agricultural countries have promoted the use of biomass sources for many household applications and biomass energy generation. Biomass is defined as the bio residue available from the sum of crop production, agricultural waste, vegetation, forestry waste and organic waste. Biomass is a renewable source of energy and contains a complex mixture of carbon, nitrogen, hydrogen, and oxygen. Various biomass resources are available in different parts of countries and are classified based on their availability. The broad classification of biomass energy is in three formats; residue of agricultural crops, energy plantations, and municipal and industrial waste. Agriculture residues are organic materials produced as the byproduct from processing and harvesting of agriculture crops. 

Crop: Plants grown in association, sown (or planted) and also harvested by human being, are collectively known as crop.
Crop plants have some economic values and there is an interaction or competition among the plants.

System is a set of principles or procedures according to which management is done; it is an organized scheme or method. A system is a group of interrelated or interacting elements (crops or livestock) that act according to a set of rules to form a unified whole.

Growing more than one crop in sequence or simultaneously as a mixed crop is an ancient practice. In Indian Vedas, there is a mention about double cropping. In early periods, the objective to adopt sequential cropping practices was to reduce the risk of total crop failure due to uncertain monsoon and to have a variety of produce to meet food requirement of farmer’s family, fodder to animals and to meet his cash requirements. Other benefits of yield increase was due to complementary effects of the component crops in improving the soil fertility, spreading the labour requirement throughout over a period of time and to reduce damage caused by weeds, pests and diseases were only incidental.

Multiple cropping in simple terms refers to the practices that increase production in a unit area of land through two or more growing crops simultaneously. The importance of intensive cropping in sustainable agriculture has been well understood by the agricultural scientists of late and there has been surge of interest in the research community to explore these complex systems in detail and to see what can be learnt from the farmers in our quest to make cropping system more sustainable progress made in the past through intensive research carried by the various agricultural universities, ICAR, research institutes/ coordinated research projects and international research institutes such as IRRI, ICRISAT, IITA and CIMMYT, it has shown that and more efficient utilization of the natural resources is possible by suitably modifying the present cropping systems in the various regions of the world.

The present cropping intensity in India is 136 per cent. There is a great prospect for increasing the cropping intensity both in irrigated and rainfed areas. In many irrigated areas, cropping intensity can be increased to 200-300 per cent. There is good scope to increase cropping intensity to 180-200 per cent with proper policies and technology options in rainfed conditions also.

Crossandra is an important tropical flowering crop valued for its attractive flowers and continuous production of loose blooms. It has particular importance in southern India, where flowers are widely used for garlands, hair decoration and religious purposes. This chapter covers the crop’s botanical characteristics, origin, distribution, genetics and commercially important varieties. It explains propagation, nursery management, climate and soil requirements, land preparation, planting, irrigation and nutrient management. Important cultural practices and plant growth management are discussed for obtaining regular flowering and good-quality blooms. The chapter also addresses insect pests, diseases, harvesting and postharvest management, including the correct method of flower collection. It provides a comprehensive production package for growers and students.

ABSTRACT

The concept and nature of intellectual property have undergone a paradigm shift during the last few decades. The importance of effective IPR governance has attracted the attention of its stakeholders. Hence, this paper focuses on the role of IPR governance and management practices for improving the quality of relationships among stakeholders besides documenting current and future trends of IPR governance and management.

INTELLECTUAL PROPERTY RIGHTS – AN INTRODUCTION

Intellectual property rights refer to the privileges granted by the government to any individual or organization for creative prowess. In recent years, such rights have gained immense importance. The laws in existence for protection and enforcement of these rights are meant to foster innovation and for regulating the use of inventions.

The integration of bioinoculants and nanoparticles presents a promising strategy for enhancing sustainable agricultural practices, yet significant challenges impede their widespread adoption. Limited awareness among farmers regarding the benefits and applications of these technologies is a critical barrier, emphasizing the need for targeted educational initiatives. Additionally, the high cost of nanoparticle and bioinoculant formulations restricts accessibility, particularly for small-scale farmers. Standardization and quality control protocols are crucial to ensure consistent efficacy, as variability in bioinoculant formulations can compromise their effectiveness. Regulatory frameworks remain complex, further hindering commercialization and large-scale implementation. Environmental concerns are also prevalent, given the potential toxicity of nanoparticles like zinc oxide and silver nanoparticles, which may disrupt soil microbial communities. Despite these challenges, future prospects for bioinoculants and nanoparticles are promising. Integrating these technologies into sustainable farming practices can reduce the reliance on synthetic fertilizers and pesticides, as seen in the use of Azospirillum brasilense encapsulated in chitosan nanoparticles to enhance drought tolerance in maize. Furthermore, the application of Pseudomonas putida encapsulated in alginate nanoparticles has effectively mitigated heavy metal stress in tomato plants. Precision agriculture, employing data analytics and remote sensing, can optimize the targeted delivery of bioinoculants and nanoparticles, maximizing crop productivity and nutrient uptake. However, comprehensive in vivo studies are essential to assess long-term efficacy and environmental impact under field conditions. Future research should focus on developing ecofriendly nanomaterials, refining controlled-release systems, and conducting

Cut greens are essential components of floral arrangements, providing structure, contrast, visual balance and supporting foliage around flowers. This chapter introduces commercially important cut foliage crops and explains their ornamental and economic significance. It covers species such as Adiantum, Aglaonema, Alocasia, Alpinia, Anthurium, Asparagus and Aspidistra, together with their cultivation requirements. Climate, shade, soil, irrigation, nutrition, weed management, pests and diseases are discussed along with harvesting, postharvest treatments, pulsing solutions and vase life. The chapter demonstrates how commercial cut-green production can complement flower enterprises and provide additional income opportunities for floriculture growers.

Introduction

Nitrogen-fixing cyanobacteria are the group of most primitive prokaryotes that have been observed in proterozoic era. Many Proterozoic oil deposits are attributed to the activity of Cyanobacteria, such as Gloeocapsomorpha. Small concentrically layered structures called pisolites are also the result of fossilized bacteria.

Introduction
Cytoplasmic male sterility (CMS), a condition under which a plant is unable to produce functional pollen, is widespread among higher plants. CMS systems represent a valuable tool in the production of hybrid seed in self-pollinating crop species, including maize, rice, cotton, and a number of vegetable crops. Hybrids often exhibit heterosis, more commonly known as hybrid vigor, whereby hybrid progeny exhibit superior growth characteristics relative to either of the parental lines. CMS systems can be of considerable value in facilitating efficient hybrid seed production.
Male-sterility-inducing cytoplasms were recognized in the 1900s. Bateson and Gairdner reported male sterility in flax in 1921, was inherited from the female parent, although genes passed from both the male and female parents affected its expression. Chittenden and Pellow (1927) recognized that male sterility in flax was due to an interaction between the cytoplasm and nucleus, with the male sterility conditioned by the homozygous recessive nuclear genotype in combination with the sterility-inducing cytoplasm.

Dahlia is a highly valued ornamental crop recognized for its large, colourful and diverse flower forms. This chapter introduces its origin, classification, botanical characteristics, genetic diversity and commercially important varieties. It discusses propagation through seeds, tubers and other methods, together with climate and soil requirements, planting, spacing, nutrient management, irrigation and crop regulation. Staking, pinching, disbudding and other cultural operations are explained for producing quality stems. The chapter also covers major insect pests, diseases, harvesting, postharvest handling, grading and yield. Its practical approach makes it useful for commercial growers and students of ornamental crop production.

Abstract

Biological databases that are composed of a large collection of digital nucleic acid sequences, protein sequences, or other polymer sequences stored on a computer, which is made available to the research community worldwide using web servers. The vast amount of information generated in molecular biology is analyzed, curated, tabulated, and arranged in databases. There are different types of biological databases, based on their contents. Besides, the database management system, a program to manage the databases, can be of two types: distributed DBMS and relational DBMS. This chapter briefly narrates the importance and purpose of biological databases.

Deep learning has significantly advanced automated plant phenotyping and disease detection by enabling computers to identify complex patterns in large volumes of plant imagery and sensor data. This chapter examines the fundamentals and applications of deep learning architectures in plant science. It discusses convolutional neural networks for image-based plant analysis and advanced architectures including recurrent neural networks, transformers and hybrid models. Data acquisition through imaging and sensor technologies is also considered. The chapter highlights how deep learning can support automated identification of plant characteristics, disease symptoms and crop health conditions. Challenges related to datasets, environmental variability, computational requirements and model generalization are examined alongside emerging research opportunities.

Deep learning emerged as a serious contender in the field from a 10-year explosive computational development. Deep learning is therefore a special form of machine learning, the algorithms of which are based on the structure and function of the human brain.

Machine learning VS Deep learning

Deep learning is arguably the most efficient form of machine learning. This is so important because they know the best way to solve the problem and how to fix everything. The following is a description of deep learning and machine Learning:

Experiment - 1
 
Aim: Molecular analysis of putative transformed plants by Polymerase Chain Reaction

The expression of transgenes are not identified at that time but it can be achieved through DNA analysis of that plant. The presence of transgene are generally identified of PCR-based detection followed by gel electrophoresis and comparison with standard samples. The main achievement of PCR-based detection is that it is very sensitive. The Polymerase Chain Reaction (PCR) provides a rapid and sensitive method to amplify a specific gene sequence. 

Electrophoresis is a method used to separate charged particles from one another based on differences in their migration speed. In the course of electrophoresis, two electrodes (typically made of an inert metal, e.g. platinum) are immersed in two separate buffer chambers. Gel electrophoresis is a technique used to separate molecules based on physical characteristics such as size, shape, or isoelectric point. A gel is composed of agarose or polyacrylamide. Agarose is used for separating DNA fragments in size range of 100bp to 20kb. Polyacrylamide is preferred for smaller DNA fragments. Separation is achieved by moving the negatively charged nucleic acid molecules through agarose matrix with an electric field. Rate of migration of DNA molecules is inversely proportional to their molecular weight. Smaller molecules move faster than larger ones. But conformation of the DNA molecule is also an important factor. Conformations of a DNA plasmid that has not been cut with a restriction enzyme will move with different speeds such as: supercoiled plasmid DNA will move fastest followed by linearised DNA, while open circular DNA will move at the slowest pace. To visualise DNA, gels are stained with ethidium bromide which intercalates in DNA and fluorescse under UV.

Experiment- 1

Aim: Direct DNA delivery to Plant by Particle Bombardment
 
In direct gene transfer method, the foreign gene of interest is delivered into the host plant cell without the help of vector. Klein and Coworkers (1987) evolved a procedure through which the delivery of DNA into cells of intact plant organs or cultured cells is done by a process called Projectile Bombardment. The microprojection (Small and high density particles) are accelerated to high velocity by particle gun apperatus. These particles with very high kinetic energy penetrate the cells and membranes and transmit foreign desired DNA inside of the bombardment cells.

Experiment - 1 
Aim: Mobilization of recombinant Ti plasmid (with gene of interest) from  
(E. coli) to an Agrobacterium tumefaciens strain. 

Principle: Due to ease of cloning and harvesting plasmids, E. coli is most popular for maintenance of plant transformation vectors. Consequently, the plant transformation vector with cloned gene of interest is mobilized to recipient Agrobacterium strain harbouring vir plasmid. Plasmid are closed, circular DNA molecules that are able for autonomous replication with a host cell. 

Experiment - 1

Aim: Isolation of plant genomic DNA through modified CTAB method

Principle: Genomic DNA is extracted from plant material requires cell lysis, inactivation of cellular nucleases and separation of the desired genomic DNA from cellular debris. Ideal lysis method is rigorous enough to disrupt the complex starting material (plant tissue), yet gentle enough to preserve the target nucleic acid. The cetyltrimethylammonium bromide (CTAB) procedure is sufficient for the extraction and purification of DNA from plants and plant derived food stuff.  This is particularly suitable for the elimination of polysaccharides and polyphenolic compounds otherwise affecting the DNA purity and hence quality.