eChapters

7.1. INTRODUCTION

The cultivation of wheat (Triticum spp.) reaches far back into history. Wheat was one of the first domesticated food crops and for 8000 years has been the basic staple food of the major civilizations of Europe, West Asia and North Africa. Today, wheat is grown on more land area than any other commercial crop and continues to be the most important food grain source for humans. Its production leads all crops, including rice, maize and potatoes.

Wheat is special in several ways. It is grown on more than 240 million ha, larger than for any other crop, and world trade is greater than for all other crops combined. The raised bread loaf is possible because the wheat kernel contains gluten, an elastic form of protein that traps minute bubbles of carbon dioxide when fermentation occurs in leavened dough, causing the dough to rise (Hanson et al., 1982). It is the best of the cereal foods and provides more nourishment for humans than any other food source. Wheat is a major diet component because of the wheat plant’s agronomic adaptability, ease of grain storage and ease of converting grain into flour for making edible, palatable, interesting and satisfying foods.

17.1. INTRODUCTION

Yam bean is one of the adaptable, low-maintenance, and nutritious food crop grown in moderate soil. It has many synonyms, in India it is known as Mishrikand (Hindi), Keshar or Ram-kaseru or Shankalu (Bengali). The root crops produced by legumes have long recognized as important and the FAO (1979) has recommended them as a source of human nutrition. The tuber is rich in starch (15-20%), 3-4% sugar, and have considerable amount of protein (2-3%). The harvested crop keeps well for months under ambient temperature. Kale (2006) observed relatively high iron contents in storage roots (up to 130 mg/kg of storage root dry matter). It can also be used for alcohol production.

The crop is very hardy, resistant to common insect pests and diseases. Most important is that the crop is not grazed neither by goats nor cows. The crop grows well in hot-humid environment and requires long, warm frost-free growing season. A moist, light textural soil is preferred for production of tuberous roots. As a legume crop, it can be grown without nitrogen fertilizer. Under good growing condition its root nodules habour symbiotic colonies of nitrogen-fixing bacteria- Rhizobium spp. (Nielsen et al., 1998b). A season crop of yam beans can release as much as 53 tons of usable nitrogen per acre into the soil. Mycorrhyzae also associated with the crop, thus facilitating phosphorus uptake. Thus, yam bean is highly suited to the needs of small farmers, and from both an ecological and a socioeconomic perspective

Introduction

Intellectual Property emerged in new type of property referring to creation of the mind in today’s globalized world. While IP such as copyrights, patents, and trademarks are centuries old, but the extension of IPRs to living beings and knowledge related to them is relatively recent. Similarly Intellectual Property Rights (hereafter IPRs) mean legal protection to protect data from new inventions. In exchange for being required to reveal the formula or strategy underlying the technique, these rights enable the owner to stop imitators from commercializing the inventions or processes for a limited time. Biological Diversity refers to all kinds of living organisms found in an area which maintain balance and support life hence may be called as the cornerstone of sustainability of all living creations i.e. the hallmark of the life of living beings since origin. In general, all organisms, including flora and living things, microbes, and their ecosystems, are together referred to as biodiversity.

Abiotic stresses include salinity, drought, flood, extremes in temperature, heavy metals, radiation etc. They are the foremost factors that causes the loss of major crop plants worldwide. This situation is going to be more rigorous due to increasing desertification of world’s terrestrial area, increasing salinization of soil and water, shortage of water resources and environmental pollution.

The inadequacy of water availability, including precipitation and soil moisture storage capacity, in quantity and distribution during the life cycle of a crop to restrict the expression of its full genetic yield potential”. Generally drought stress occurs when the available water in the soil is reduced and atmospheric conditions cause continuous loss of water by transpiration or evaporation. Drought stress tolerance is seen in almost all plants but its extent varies from species to species and even within species.

Unique Achievement in Plant Breeding

Rice

TN-1: First dwarf variety of rice developed in world

IR-8: Second dwarf and first high yielding variety of rice developed in world

Jaya: First dwarf variety of rice developed in India

Introduction

Rhizobia and Frankia are soil bacteria that exhibit symbiosis with their particular hosts owing to their ability to understand the intricate signals excreted by the host roots. Rhizobia exhibit symbiosis with legumes and one species member of Ulmaceae family Parasponia andersonii while actinomycetes Frankia forms the group of nitrogen-fixing bacteria that live in symbiosis with a large spectrum of dicot plants distributed among eight families consisting of over 24 genera and around 200 species of angiosperms (Benson and Silvester, 1993) and are collectively responsible for 15% of the biologically fixed nitrogen in the world. This Frankia-woody tree or dicotyledonous plant interaction results in evolution of the actinorhizal plants and the symbiotic interaction is described between Frankia (actino) and plant roots (rhiza).

The success of a TTO relies on a systematic approach encircling factors like assessment, valuation, marketing, negotiation, and post-transfer management (fi gure 8). This chapter covers the activities that are essential for the effective functioning of a TTO. Other activies Technology Assessement /Valuation Financial Managemnt Major Activities of technology transfer office Commercialization IP Management Fostering Innovation
Fig 8: Various activities of TTOs.
3.1 Technology assessment - A pillar of innovation commercialization
The process of transitioning an innovative technology to commercial reality requires a stringent and formal process known as technology assessment. This early stage is crucial as it determines the viability of an invention by analysing practicability of the invention as well as its uniqueness and the likelihood of it being adopted in the target markets. In the absence of this preliminary examination, the chance of technology’s potential being mismatched to the market can arise.
a) Scientific merit and technical feasibility
A key benchmark in assessing technologies involves the identification of
scientific validity and technical performance. An innovation’s viability depends on the fact that it complies with good scientific practice or the innovative application of a new paradigm that has empirically confirmable validity. It is important to determine whether the invention’s underlying mechanism can be reliably proven through established scientific practice. A working prototype allows for empirical testing in different conditions and serves as proof of practical application. In addition to that, scalability has to be tested critically. It often happens that innovations perform well in a controlled laboratory setting but lose efficiency or cost-effectiveness in the case of mass production. The viability of the implementation on a grand scale becomes a core concern in this phase of the evaluation.

The whole genome sequencing helps the researcher to identify the desired gene and make it useful for the breeding program by transferring that particular gene into improved cultivar. Since, the whole genome sequence is not available for all crops and animals; there is a need of advanced techniques to overcome this problem. One such technique is TILLING (Targeting Induced Local Lesions IN Genomes) and its related technique called Eco-TILLING. TILLING is a reverse genetic approach used to identify the point mutation created by chemical mutagen in the mutagenized population. The chemical mutagen is most widely used and it will create allelic series of mutations which include nonsense, missense, or splice site mutation. The individuals with mutation were pooled by two-dimensional or three-dimensional pooling techniques. These mutations were identified by the formation of heteroduplex complex in the mutated region of the gene. Then, the heteroduplex is cleaved by S1 nuclease family enzyme and were examined in gel electrophoresis which distinguishes heteroduplex from homoduplexes. Now, the identified mutants in gel electrophoresis were subjected to sequencing for confirmation. Eco TILLING is similar to the TILLING except for the mutant population, as we use the natural mutant population in Eco-TILLING; whereas in TILLING we use the artificial mutant population and also pooling is done with reference sample in Eco-TILLING. The advantage of TILLING is that it is a cost-effective, rapid screening of mutant population and a nontransgenic approach as it does not require biosafety measures.

Introduction

Sugarcane is a vital commercial crop of our country that occupies around 5.11 million ha of land with an annual cane production of around 400.16 million tonnes and yield of 78.25 t/ha. Sugarcane occupies 2.67% of the cultivated land area and about 7.5% to the agricultural production in the country (DAC, 2020). This crop is a source of earning and livelihood for about 35 million farmers and approximately equal number of labourers. After textile industry, the sugar industry holds its place for the largest agro-based industry in country. Around 40-50% of the cane is utilised in 435 sugar industries present in India for the manufacture of about 15 million tonnes of sugar.

This crop is also a source of raw material for two important small scale industries, i.e., gur and khandsari. These industries utilises about 50-55% of the sugarcane produced for production of about 10 million tonnes of these products altogether. Besides, sugarcane crop is also the producer of molasses, chief by product, which is the main source for production of alcohol. In the year 2018-19, about 13.79 million tonnes of molasses is produced by our country. Apart from this, the left over fibrous material of sugarcane termed as sugarcane bagasse is also a source of production of electricity in the sugar mills. The left over extra bagasse is now-a-days utilised for even production of paper in paper industries. Also, co-generation of power by this product of sugarcane as fuel is considered to be best practical thing in most of the sugar mills. Using this as a raw material, about 3500 MW power may be generated each year without the use of any extra fuel and money being less than normally utilised in case of fuel production from thermal powers. Press mud is also one of the by-products obtained from sugarcane which is considered to be rich source of organic matter and micro and macro nutrients. Even the green tops of sugarcane are being used as cattle fodder. Besides, the cool sugarcane juice full of energy is in great demands in urban area to quench the thirst of people, especially in hot summer seasons (Anonymous, 2008). The trend of sugarcane production over the years is given

The income of farmers is declining day by day due to increasing cost of inputs including wages of farm labor. The net income can only be increased by reducing cost of inputs and by increasing yield of currently adopted cropping systems. The income of farm can be increased by growing horticultural crops (vegetables, flowers, fruits etc.) as well as medicinal, aromatic or spice crops. However, these crops cannot be grown on large scale due to their market problems and involvement of high labor. So, in order to improve profit from ongoing cropping systems, some latest crop production practices are being discussed in this chapter.

Two cornerstone methodologies in genetics, forward genetics and reverse genetics, illuminate the intricate connection between gene sequences and their functions. Forward genetics traditionally entailed laborious steps, beginning with the observation of mutants and culminating in the identification of causal genetic alterations. While effective, this approach was time-consuming and limited in scalability. Recent innovations have revolutionized forward genetics with next-generation sequencing (NGS), genome-wide association studies (GWAS), CRISPR (clustered regularly interspaced short palindromic repeats)-based screens, and advanced computational techniques. These advancements deliver precision and efficiency, enabling researchers to explore gene function on an unprecedented scale. In contrast, reverse genetics initiates its journey armed with sequence data and harnesses the manipulation of genes to observe resultant phenotypic shifts. Traditional reverse genetics, though invaluable, faced challenges like limited precision and scalability. Cutting-edge approaches have transformed the landscape. Techniques such as prime editing, base editing, high-throughput functional genomics, single-cell genomics, and synthetic biology offer unparalleled precision and capacity. They empower scientists to scrutinize gene function at the single-cell level, conducting precise genome editing and large-scale functional genomics studies. These strides in forward and reverse genetics have charted new territories for genetic research.

Introduction

Farm mechanization is application of machine power to work on land, usually performed by animate and mechanical power. It is also defined as an economic application of engineering technology to increase the labour efficiency and productivity. However, it not only includes production, distribution and utilization of a variety of tools, machinery and equipment but also planting, harvesting and primary process. Mechanization has a major impact on demand and supply of farm labour, agricultural profitability and a change in rural dynamics.

Introduction

Livestock is an important component of Indian agriculture contributing 28% of agricultural gross value added (GVA) of the country. Agriculture, as such, contributes 16.4% of country's GVA (Economic survey, 2017-18). Livestock as a source of income has seen a steady increase from 4% to 13% in a decade time (2002-2012). India, with 2.29% of the world land area, is maintaining about 10.71% of the world’s livestock population. The milk production in the country increased from 17 million tonnes in 1950-51 to 187.7 million tonnes in 2018-19. The feed and fodder play an essential role in the health and productivity of livestock. Even though India is the world largest milk producer with 21% of global production, the productivity (1700 kg/year) is far below the world average (2574 kg/year) (FAO STAT, 2018). One of the main responsible factors (50.2%) for this low productivity is the deficiency in feed and fodder. Although forage crops are the critical component of livestock-based farming system, the area under cultivated fodder is static to around 8.4 million ha (5.23%) since last two decades due to the competition of land use with other crops. Availability of the fodder determines the productivity and profitability of the livestock rearing because fodder and feeds constitute about 60% of the total cost of milk production, which can be lowered by enhancing the green fodder-based feeding system. The traditional grazing lands are gradually diminishing because of urbanization, expansion of the cultivable area, grazing pressure, industrialization etc. These factors coupled with stagnation of area under cultivated fodder resulted in a severe shortage of feed and fodder to the extent of 26% in dry crop residues, 35.6% in green fodder and 41% of concentrates (IGFRI, 2011). This gap in demand and supply may further rise due to consistent growth of livestock population at the rate of 1.23% in the coming years. The share of the in-milk population to total population has increased from 23% to 27% in the case of cattle and from 33.64 to 34.74% in buffaloes from 2012 to 2019. The year-on-year growth rate of milk is approximate 6%. Thus, to sustain this growth rate and for further expansion to meet the demands of the ever growing human population, livestock needs a sustainable supply of feed material. To reduce the demand and supply gap, the production and productivity of fodder crops need to be enhanced. The productivity of cultivated fodder crops is low, due to the least attention and allocation of minimal production resources and lack of dissemination of the production techniques to stakeholders involved in the forage resource development. The present chapter highlights the recent advances in the forage crop production technologies. The comprehensive compilation of information at one place provides the opportunity to get the holistic picture of forage production altogether.

Nutrient use efficiency of crops is being improved through temporal and spatial management of the form and amount of nutrient inputs as being demonstrated by various agronomic practices. In addition, understanding of the genetic basis of nutrient efficiency would lead to the development of nutrient use efficient varieties thus reducing the application of nutrients. Earlier studies on genetics of nutrient use efficiency were limited as increasing productivity with heavy nutrient inputs was the main focus. But in the backdrop of environmental degradation due to the excessive nutrients and its impact in climate change, nutrient use efficiency in crops is need of the hour for sustainable and eco-friendly agriculture.

Introduction
Crop breeding programmes are under increasing pressure for developing cultivars that possess the ability to adapt to diverse environments while maintaining higher quality and quantity, in response to the escalating worldwide demand for food. The primary goal of plant or crop breeding is to attain genetic enhancement, leading to the creation of new varieties that demonstrate higher yield and superior quality. Hence, the central aim of crop breeding initiatives is to develop novel cultivars that demonstrate improved resistance against fungal, bacterial, insect, or viral pathogens, alongside increased tolerance to abiotic stresses such as drought low temperature, salinity, dehydration, and heavy metal toxicity. Additionally these breeding efforts aim to improve quality attributes, including taste, size shape, colour, and cooking convenience (Lenaerts et al., 2019). The ability to anticipate emergent phenotypes resulting from genetic or environmental disruptions necessitates the utilization of methodologies capable of identifying metabolic pathways or canopy structures suitable for change. The insilico tools have the potential to guide breeding endeavours aimed at developing novel germplasm that exhibits robust adaptation to diverse environmental conditions. The precise simulation of organisms in a computational environment is a current and effective approach to enhance prediction abilities. Significant progress has been made in the field of predictive medicine through the implementation of community projects that utilize integrative, multi-scale modeling techniques. However, there are a lot of crop models that are just geographically and temporally restricted, which limits their ability to go beyond the data that is now available and, consequently, leads to insufficient forecasts regarding crop reactions in novel circumstances. The plant community has launched or continued a coordinated endeavour focused on the development of a virtual plant modelling through consolidative and multi-scale mapping, models and simulations (Marshall-Colon et al., 2017).

Introduction

Maize (Zea mays L.) is one of the most versatile promising crops having wider adaptability under diverse agro-climatic conditions. Worldwide, maize is known as “Queen of cereals” because it has the maximum genetic yield potential amongst the cereals. It is cultivated on nearly 197 million ha in about 170 countries having wider diversity of climate, soil, biodiversity and management practices that contributes 36% (1135 million tonnes) of the global grain production. The United States of America (U.S.A.) is the largest producer of maize contributes nearly 33% of the total production in the world and other important growing countries are China, Brazil, India, Argentina, Indonesia, Ukraine and Mexico (Table 1).

Introduction

The various kinds of plants present on earth have been used over the millennia for human welfare in the promotion of health and as drugs and fragrance materials. About 80% of the world’s inhabitants rely mainly on herbal medicines for their primary health care, while medicinal plants continue to play an important role in the health care systems of the remaining 20%. The use of herbal medicine and medicinal plants in most developing countries, as a normative basis for the maintenance of good health, has been widely observed (UNESCO, 1996). According to WHO (1998), herbal medicines are finished, labelled medicinal products that contain as active ingredients, above ground or underground parts of plants or other plant materials, or combinations thereof, whether in the crude state or as plant preparations. The plant materials referred to above include juices, gums, resins, fatty oils, essential oils and any other substances of this nature. Herbal medicines may contain excipients in addition to the active ingredients (WHO, 1991). Remarkably, even today there is no real definition for this special group of plants that has been accompanying mankind throughout history. Most frequently, medicinal plants are defined as feral and/or cultivated plants that, based on tradition and literature records, can be directly or indirectly used for medical purposes. The basis of medicinal use is that these plants contain so called active ingredients (active principles or biologically active principles) that affect physiological (metabolic) processes of living organisms, including human beings.

The conception about aromatic plants is even less definite. The term aromatic indicates plants having an aroma; being fragrant or sweet-smelling, while the word aroma is supposed to imply also the taste of the material (aromatic herbs). Since, large number of plants possess both medicinal and aroma properties which leads to complexity and overlapping uses of active ingredients, making impossible to establish rigid categories or a practical classification for medicinal and aromatic plants. For example; Anise, dill, coriander, thyme, etc. are equally known as medicinal, spice and essential oil crops. Thus, frequently these plants are simply referred to as medicinal plants, disregarding their specific features. More recently, the term “Medicinal and Aromatic Plants” (MAPs) has been used in a slightly broader sense, distinguishing the fragrant (aromatic, ethereal) ingredients containing group of medicinal plants.

1. Introduction
Fish and fisheries remains to be an important sector for Indian economy providing food, nutrition as well as employment to a large share of the population. Initiating with a production of merely 7.7 lakh ton of fishes in 1951-52, the country was on its way of increasing the production steadily and has now attained the phenomenal success of producing 162.5 lakh ton during 2021-22. Of this total amount, 41.3 lakh ton has been obtained from marine fisheries and 121.2 lakh ton from inland resources (Anon, 2022). For the inland fisheries production, the contribution of fish culture sector was much lower than that from capture fisheries for several decades during the initial phases. However, the production of culture fisheries maintained a consistently increasing trend and has now surpassed in magnitude the production from capture fisheries sector (Fig.1). With the potential areas for fish culture being gradually used up for different purposes, maintaining such high production rates in coming days will be possible only through optimum management of various factors which govern the productivity of the ponds. Among such factors, one important aspect is nourishing the quality of pond soil and water.

Introduction

Oilseeds are the third most important group of crops after cereals and pulses in India. These are important food crops which also find their place in industrial use. Oilseeds have been an integral component of Indian agriculture since time immemorial. On the oilseeds map of the world, India occupies a prominent position, both in terms of acreage and production. India is the 4^th largest edible oil economy in the world and contributes about 10% of the world oilseeds production, 6-7% of the global production of vegetable oil, and nearly 7% of protein meal. The oilseeds production statistics of the world for the year 2016-17 includes a production of 549.98 million tonnes from an area of 234.57 million ha with the productivity of 2.34 tonnes/ha. The top countries include U.S.A., China and India with U.S.A. ranking first in all area, production and productivity of 39.25 million ha, 126.94 million tonnes and 3.23 tonnes/ha, respectively. Next is China which ranks 2^nd in production and productivity but 3^rd in terms of area; the area, production and productivity being 22.72 million ha, 54.92 million tonnes and 2.42 tonnes/ha, respectively. India ranks 2^nd in terms of area, i.e. 33.83 million ha but 3^rd in both production and productivity being 36.32 million metric tonnes and 1.07 tonnes/ha respectively.

Low bioavailability of phosphorus (P) in soils is one of the major limiting factors influencing crop production throughout the world. Plants, however, are known to possess potential adaptive mechanisms at morphological, physiological, biochemical, and molecular levels to overcome P deficiency (Vance et al., 2003). Such adaptive mechanisms mainly include an increase in total root length and root hair growth (Bates and Lynch, 2000), enhanced organic acid (Singh and Pandey, 2003), acid phosphatase (Pandey, 2006) and ribonuclease (RNase) secretion into the rhizosphere (Hocking, 2001), increase in expression of proteins such as phosphatase, inorganic phosphate (Pi) transporter, RNase and phosphoenolpyruvate carboxylase (PEPcase) in plant tissues.