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Abiotic stresses include salinity, drought, flood, extremes in temperature, heavy metals, radiation etc. They are the foremost factors that causes the loss of major crop plants worldwide. This situation is going to be more rigorous due to increasing desertification of world’s terrestrial area, increasing salinization of soil and water, shortage of water resources and environmental pollution.

The inadequacy of water availability, including precipitation and soil moisture storage capacity, in quantity and distribution during the life cycle of a crop to restrict the expression of its full genetic yield potential”. Generally drought stress occurs when the available water in the soil is reduced and atmospheric conditions cause continuous loss of water by transpiration or evaporation. Drought stress tolerance is seen in almost all plants but its extent varies from species to species and even within species.

The disease , referred as the disorder of crop plants caused by fungi, mycoplasma, bacteria and viruses. Plant resistance is one of the most effective and ideal approach to combat diseases and insect-pest. Resistance is the capacity of plants to resist, withstand and overcome the attack of pathotypes.

Mechanism of Disease Resistance

Different mechanism of disease resistance are as under:

1. Disease escape

The ability of susceptible host plants to avoid attack of disease due to environmental factors like early maturing varieties, shift in the date of planting, change the field, balanced application of NPK etc. Example., early maturing varieties of groundnut and potato may escape from ‘Tikka’ and ‘Late blight’ diseases respectively.

2. Disease tolerance

The ability of the plants to tolerate the attack of the pathogen without loss or little loss in the yield. This endurance is brought about by the influence of external characters. Generally, tolerance is difficult to measure since it is confounded with partial resistance and disease escape. Disease tolerance is of three types:

Crop breeders are continuously engaged in the improvement and development of crop varieties, in search of a genotype which will prove superior to the existence cultivars. A strain is duly released by appropriate duly empowered legal organization. Only when it is officially released, it is called variety, Before release, it is said to be strain or line. The release of new crop varieties consists of the following major steps:

1. Development of new strains

The new strains are developed by ICAR Crop Research Institutes and State Agricultural Universities for specific purposes. Various breeding methods are used for development of new strains. Some varieties are developed by selection without hybridization such as pure-line variety, mass selected variety and clonal variety. Development of some varieties involves hybridization and selection. This includes varieties that are developed by pedigree, bulk and backcross methods including multiline cultivars and population improvement methods.

2. Evaluation of performance

This trial is conducted at the research station, where new strains has breed. The object is to make sure that new strains are better in performance than the existence variety, so that it can be promoted to the trials where new strains has to be compete with other strains, developed by other scientists/researchers.

The term ideotype was proposed by Donald in 1968. Ideotype is a biological model which is expected to perform in a predicted manner in a defined environment. A crop ideotype is a plant model that is expected to perform better in a particular environment. The concept of ideotype changes from crop to crop, climate to climate and the concepts and policies of the community concerned. Ideotype breeding is the development of a plant type, expected more quantity of grains, with ideal character. Plant characters are selected from an agronomic point of view.

Molecular markers, including DNA based genetic markers have found their applications in almost all areas of biology, more so in evolutionary biology, genetics and breeding in order to identify a particular sequence of DNA in a pool of unknown DNA. For many applications, DNA markers are preferred over other molecular markers since DNA is stable within an organism over time and among stages of development whereas other molecules are dynamic. A molecular marker is a DNA sequence that is readily detected and whose inheritance can be easily monitored. The uses of molecular markers are based on the naturally occurring DNA polymorphism. Most commonly used molecular markers in biological science include:

1. Restriction Fragment Length Polymorphism (RFLP)

RFLP is the first molecular marker technique which was invented by Botstein et al. (1980). The technique is based on restriction fragment length polymorphism; a molecular marker based on the differential hybridization of cloned DNA to DNA fragments in a sample of restriction enzyme digested DNAs; the marker is specific to a single clone/restriction enzyme combination. It consists of the following steps:

Quantitative traits are governed by many genes or polygenes genes with small individual effects. They are often described by their gene action rather than by the number of genes by which they are governed. There are four types of gene action: additive, dominance, epistasis, and overdominance. Because gene effects do not always fall into clear-cut categories, and it should be pointed out that gene action is conceptually the same for major genes as well as minor genes, the essential difference being that the action of a minor gene is small and significantly influenced by the environment. A general way of distinguishing between these types of gene action based on interaction among alleles is as follows:

While dealing with polygenic traits and the continuous variation in plant breeding programs, it is important to know as to what extent the phenotype is decided by the genotype and to what extent by the environment. It is well known fact that what we observe i.e. phenotype is the result of genotype, environment and interaction between the genotype and the environment. The total variance is phenotypic variance or the variance of phenotypic values and various components of variance are as shown in Table 6.1.

The response to selection (R) or selection advance or genetic gain is the difference between the mean phenotypic value of the offspring of the selected parents and the whole of the parental generation before selection. The response to selection is simply the change of population mean between generations following selection. Similarly, the selection differential (S) is the mean phenotypic value of the individuals selected as parents expressed as a deviation from the population mean (i.e., from the mean phenotypic value of all the individuals in the parental generation before selection). Response to selection is related to heritability by the following equation:

Random Mating

Random mating occurs when each female gamete has an equal chance of being fertilized by any male gamete of the same plant or with any other plant of the population and, furthermore, there is an equal chance for seed production. It is not possible to achieve true random mating in plant breeding because selection is involved. Consequently, it is more realistic to describe the system of mating as random mating with selection. Whereas true random mating does not change gene frequencies, existing variability in the population or genetic correlation between close relatives, random mating with selection changes gene frequencies and the mean of the population, with little or no effect on homozygosity, population variance, or genetic correlation between close relatives in a large population.

To start with let the self-incompatibility be defined.

• Self-incompatibility is the mechanism by which plant can prevent selffertilization.
• Self-incompatibility is defined as inability of a plant producing functional gamete to achieve self-fertilization.
• Self-incompatibility is a genetically based mechanism which controls mating within angiosperm species by imposing a ‘barrier’ between pollination and fertilization.
• The term incompatibility is used to describe the failure of pollen tube to penetrate the full length of the style and to effect fertilization.

A line homozygous at all loci, ordinarily obtained by successive self-fertilizations in plant breeding is called a pure line. This concept was proposed by Danish biologist, W.L. Johannsen in 1903 while conducting a series of selection experiments for seed weight in common bean, which is strictly self-pollinated species. This is applied in self-pollinated crops, like wheat, rice, pulses, several vegetable crops, namely, tomato, chilli, eggplant, okra, cucurbits, etc. This is also applied to develop inbred parental lines in maize and several other cross-pollinated crops. However, ultimately these parental lines are used in development of new hybrids. This could be summarized as follows:

• Lines that are genetically different may be successfully isolated from within a population of mixed genetic types.
• Any variation that occurs within a pure line is not heritable but due to environmental factors only. Consequently, as Johansen’s bean study showed, further selection within the line is not effective.

A variety produced by crossing inter se a number of genotypes selected for good general combining ability in all hybrid combinations with subsequent maintenance of the variety by open-pollination. Synthetics development is common in cross- pollinated crops in the early phases of breeding. The synthetic method of breeding is suitable for improving cross-fertilized crops. It is widely used to breed forage species. Successful synthetic cultivars have been bred for corn, sugar beets, and other species like cauliflower in India. The suitability of forage species for this method of breeding stems from several biological factors. Forages have perfect flowers, making it difficult to produce hybrid seed for commercial use. The use of male sterility may facilitate controlled cross-pollination, which is difficult to achieve in most forage species. To test individual plants for use in producing the commercial seed, it is essential to obtain sufficient seed from these plants. The amount of seed obtained from single plants of these species is often inadequate for a progeny test. Furthermore, forage species often exhibit self-incompatibility, a condition that inhibits the production of selfed seed. Synthetic cultivars are also used as gene pools in breeding progeny. Synthetic cultivars are advantageous in agricultural production systems where farmers routinely save seed for planting. One of the well-known and widely used synthetic has been the Iowa stiff- stalk synthetic (BSSS) of maize.

Composites defined as advanced generation populations maintained by random mating in the material constituted through mixing of equal quantity of seeds of crosses showing less inbreeding depression in F2 over F1. The components of composites inbreds, open-pollinated populations, locally available open-pollinated cultivars, hybrids, advanced generation lines, improved populations. Mixing the seeds of several phenotypically outstanding lines produces a composite variety and encouraging open pollination to produce crosses in all combinations among, the mixed lines. The lines used to produce a composite variety are rarely or not tested for combining ability with each other like as done in case of synthetics where the combining ability of the component lines is invariably tested for GCA. Composite are commercial varieties and are maintained by open - pollination in isolation. While mixing the seeds of various genotypes, care should be taken that the component lines are similar in maturity height, seed size, colour, etc. so that the composite maintained through random mating in isolation manifests a reasonable degree of phenotypic uniformity. The variety is maintained by open pollination. Farmers can use their own seed for 3 to 4 years.

Bioinformatics is a recently emerged frontier discipline, which helps in analyzing biological data with the help of information technology and statistical software packages. In a broader sense, bioinformatics is the application of computer technology in management of biological information. This interdisciplinary scientific tool having no barriers between different branches of science like biology, biotechnology, mathematics, computer science and information technology, has great influence on advanced bio-science research, particularly dealing with genes, proteins and cell biology.

Bioinformatics is the branch of study that deals with the use of information technology (IT) for managing data of biological science. Bioinformatics drew public attention with the announcement of the draft sequence of human genome by the then US President Bill Clinton along with Craig Venter and Francis Collins in 2000 (Uma and Sathyanathan, 2004).

Abstract

The two most important tasks for a patentee to undertake after being granted a patent, in any patent regime, would be to pay the renewal fees necessary to keep the patent alive, and provide proof of having worked the patent in the jurisdiction. This article seeks to highlight features of India’s legal requirements with regard to the working of patents on a commercial scale. The number of patents granted in a country is an indicator of the technological strength of the country. However, it should be noted that actual technological growth depends upon the working of patents. Working of a patent means the commercial exploitation of the invention that is embodied in the patent.

Soil is formed by disintegration and decomposition of rocks, minerals and organic matter, Soil is a habitant for plant growth and serves as a storehouse of water and food nutrients for plants, Soil is not same everywhere and varies morphologically, physically, chemically and mineralogically from one point to another both in horizontal and vertical directions. Development of soil informatics is necessary to classify the soils, to assess the productivity of soils for various crop productions and to use the soil for various purposes such as land capability classification, soil irrigability classification, soil rating and soil mapping.

Morphologically soil may appear red, yellow, black, brown or ashy in colour. Soil may vary from sandy to clayey in texture. Depending upon the nutrient reserve capacity, the soil may be fertile or infertile. On the basis of climate, a soil may be classified as laterite soil. Red earth and red loam soil, podzol soil or desert soil.

Different kinds of soil contain minerals of various types and amounts and so exhibit distinct physio co-chemical properties. Although various methods have been used in the past to classify the soils, the most modern and scientific methods is based on dominants pedogenic processes acting on a place to from a soil. The new method of soil classification has two important characteristics-Firstly soil classification is based on certain diagnostic parameters which could be estimated quantatively, and secondly depending on diagnostic parameters, a scientific method is being followed to classify a soil from higher to lower levels such as order, suborder, great group, subgroup, family and series.

If the 1990s were the decade of genomics, the first ten years of the new century are set to become the decade of proteomics. For the first time, the technologies of proteomics make it possible to generate quantitative protein expression data on a scale and sensitivity comparable to that achieved at the genetic level. This advance has major implications for our understanding of cellular organisation in health and disease and for pharmaceutical and agricultural biotechnology. Indeed, proteomics is already yielding important findings, across a wide range of applications. Most proteomics research, however, is directed towards the more proximal goal of investigating protein expression and function under specified physiological conditions.

The rapid evolution of proteomics has continued during the past years with a series of innovations in the core technologies of two-dimensional electrophoresis and mass spectrometry, and a diversity of productive research programmes. Well annotated proteomics databases are now emerging in a number of fields to provide a platform for systematic research, with particularly promising progress in clinical applications such as cardiology and oncology. Large-scale quantitative research, comparable in power and sensitivity to that achieved for gene expression, is thus becoming a reality at the protein level.

Gene silencing is the suppression of gene expression through nucleotide sequence-specific interactions that are mediated by RNA. Many manifestations of RNA silencing-RNA interference (RNAi) in animals, quelling in fungi and post-transcriptional gene silencing (PTGS) in plants, appear to be related. RNA silencing is experimentally activated by double-stranded RNA (ds RNA) and is used as a powerful technique for specific inhibition of gene expression in a variety of organisms. In a natural context, ds RNA may be produced from rearranged loci by transcription from converging promoters or by host-or viral-encoded RNA-dependent RNA polymerases (RdRP). A key component of RNA silencing is 21-23 nt RNA known as short interfering RNA (Si RNA). In Drosophila, the siRNA is derived from ds RNA by the action of an RNase-III like enzyme named DICER. The siRNA guides a multi-subunit endnuclease, referred to as the RNA-induced silencing complex (RISC) and it specifically degrades RNA that share sequence similarly with the ds RNA.

Biotechnology provides significant opportunities and greater effects in horticulture, where even minor changes such as quality, colour, aroma, flavour, structure, disease-pest resistance and postharvest behaviour bring tremendous achievements commercially. Tissue culture, genetic engineering, molecular breeding, biofertilizers, biopesticides and postharvest biotechnology are important aspects of biotechnology in horticulture such as fruit, vegetable, ornamental, spice, medicinal, aromatic and plantation crops.

Tissue Culture

Tissue culture is the true reality of the plant biotechnology. The annual demand of tissue cultured production constitutes nearly 10 per cent of the total world biotech business, amounting to 15 billion US dollars. The global production of tissue culture industry is expanding @ 15% per annum (Chadha, 2000). In India, more than 130 tissue culture units are presently producing > 300 million plantlets per annum (Choudhury and Tejaswini, 2000).

Introduction

In the perspective of the accelerating national growth rate of 8% recorded at the end of the 10th Plan to a projected growth rate of 10% by the end of the 11th Plan, yielding an average GDP growth rate of about 9% it was appearing that the nation is going to have a strong footing of development. However, it could not be fully achieved due to several reasons including the global recession and other factors related to international economic setback which may deter achieving a projected growth rate of 10% in the 12th Plan. In consideration of the international scenario of growing complicacy in economic growth more emphasis should be given on further efficient utilization of natural resources and intellectual properties of grass root innovators to improve per capita income leading to a promising national structure of economics ensuring wide spread benefits to the society. In this regard, true development of a nation like India can be primarily realized by recognition of wide spread enthusiasm of its innovative human resources living in village and rural areas. It can be made possible only through working with them by appreciating their struggle for survival using all round innovative measures coming out of their daily life experiences and traditional knowledge. Technopreneur Promotion Programme (TePP) is a unique scheme of Government of India for development of society especially by promoting the intellectual properties of grass- root level innovators coming out from the society with a source of inspiration arising out of their daily life struggle for existence. In spite of its limited publicity in remote areas an awareness of this programme has often been found to raise great hope to the villagers to do something for their society exploring the ideas generated from the experiences of life with the help of local resources. Such naturally grown innovation can be found undoubtedly novel and the idea can change their world of dreaming for a more prosperous life by bringing changes in socioeconomic pattern of trade and employment in rural areas with proper support from Government if India through the scheme like TePP.