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The importance of Plant Pathology has been realized over the years owing to number of crop failures in different parts of the world. Plant disease control is indispensable for qualitative production and to compete in global agricultural trade. There is great need of sensitization of our research in various aspects which shall guide the generation to come for managing plant diseases not only for enhancing crop production but also in facilitating trade by conserving the biodiversity and environment.

Research Trend in Plant Pathology

Like in other sciences, there has been a change in the trend of research in Plant Pathology with passage of time. Up to the late 1960s most of the interest in plant pathology was concentrated in crop diseases mostly caused by fungi. Physiology of microorganisms and physiology of parasitism became increasingly popular fields of interest in 1960s.

The advancements in the field of informatics, biology and allied fields of technology led to the emergence of an inter-disciplinary field known as ‘Bioinformatics’. This field is an expanding and challenging tool for the collection, storage, organization, integration, analysis and simulation of biological data for utilization in biotechnology.

Collection of Data for Bioinformatics

An epoch making revolution in Genomics in recent years in deciphering plant, animal and microbial genome sequences, Proteomics, Statistics, Instrumental techniques, Structural and Computational Biology have given input to fragmented pieces-and-bits of information on biological materials. Each data is a discrete piece of important but defragmented information regarding a molecule (protein, RNA, DNA, sugars or lipids etc) or a reaction or at best a pathway, supposed to be occurring in a living system independently at a given time and space. However, as in a living system each molecule or a process exists in unison with other entities of its kind, these fragmented data are to be stored, organized, and then integrated and analysed to form an integrated part of a whole chain of events – now called a ‘SYSTEM’. Storage and organisation of data Explosion of knowledge in the field of biology and generation of new informations (data) needs well storage, organization and a faster distribution among the scientific workers. The advent of super computers, high speed computing and development of algorithms for data formatting, assists in the storage of data at the site of origin and distributed through the hyperlinks in world wide web (www).

ABSTRACT

This paper presents an outline on the patenting system prevailing in India. 

INTRODUCTION

Ideas and knowledge are increasingly important parts of trade. Most of the value of new medicines and other high technology products lies in the amount of invention, innovation, research, design and testing involved. Hence, protection of such values is paramount. This paper details how these values are protected in India.

Drug designing is the approach of finding drugs by design based on their biological targets. The substance used for medical purpose, either alone or in mixture that changes the state or function of cells, organs or organism as a whole is known as a drug. So typically a drug target is a key molecule involved in particular metabolic or signaling pathway that is specific to disease condition or pathology, or to the infectivity or survival of a microbial pathogen. Drugs may be designed that bind to the active region and inhibit the key molecule that causes disease. However these drugs would also have to be designed in such a way that they should not affect any other important molecules similar to the key molecule. These can also be used to enhance the normal pathway of metabolism by promoting specific molecules that may have been suppressed in disease state.

ABSTRACT

This paper presents few measures to be taken up by academic institutions, professional societies, industrial bodies and governmental agencies for the propagation of information of IPR in terms of encouragement of publication of books on IPR, establishment of IPR cells in academic and professional institutions and making IPR education as part of curriculum in Universities and institutions of higher learning.

A detailed treatment on the concepts of mutagenic effectiveness and efficiency was given by Konzak et al. (1965). They proposed the term “effectiveness” as a measure of gene mutations in relation to the dose and “efficiency” as an estimate of the mutation rate in relation to other biological effects induced, such as lethality, injury or sterility. To obtain high efficiency, the mutagenic effect must greatly surpass other effects in the cell, such as chromosomal aberrations, and toxic effects which generally lead to damage

Germination between M₁ and M₂ had a non-significant relationship in germination between M₁ and its corresponding M₁ progenies in M₂ (Table 27). Probably the reduction in the germination in M₁ might be very highly and significantly influenced by the conditions prevailing in germination and physiological disturbances that would be strictly associated in M₁ alone. Whereas the survival of the seedlings between M₁ and M₂ showed a strong positive relationship. The differential behaviour of the mutagens affecting germination and survival was also evident as discussed elsewhere.

However, seedling height on 10^th day sorted a differential relationship between M₁ and M₂. It was negative and non-significant. A non-significant relationship was exhibited for seedling height on 30^th day between M₁ and M₂ in all the doses. It may be interpreted that extent of physiological disturbances that occurred in the early stages and the extend of recovery mechanism that operated subsequently may be the major cause for this; not withstanding the haplontic and diplontic selection that operated in the biological system. Further the type of physiological disturbances happened, whether the enzyme systems affected or a substantial amount of toxic substances produced besides the genetical causes have not been studied in the present investigation. Moreover if the reduced growth was due to mitotic arrest in the meristamatic regions of the roots of M₁ plants, the disorder may not be completely inherited to its offspring’s (M₂ plants), in that sense selection at the cell level damages might occur in the M₁ itself and the selection for a cell with normal mitosis and extent of chromosomal damages that will be tolerated alone might be selected and hence the effect in M₁ need not be always related with M₂; it also depends upon the potency of the cell to recover from the mutagen induced damages. The ability of the cell in general, should be more in M₁ than in M₂ recover the disorder after several cell generations.

Polygenic characters exhibit a wide range of expression but the action of genes may be either additive or non-additive. According to Fisher et al. (1932), Smith (1936) Lush (1945), Panse (1946) and Mather (1949) the genetic advance can be achieved through selection of the heritable variation in only due to additive. So, selection for a particular character based on phenotypic variability without estimating the heritable part of it will not be successful. Heritability estimates along with genetic advance are normally more helpful in predicting the gain under selection than heritability estimates alone (Singh, 1997). Therefore, the heritability enters into every formula connected with breeding methods and many practical decisions are taken considering the heritability value.

The frequency distribution of the populations of M₂ and M₃ generation for each genotype was extracted according to Panse and Sukhatme (1961). Skewness and Kurtosis for all the characters were calculated as per the standard method. Skewness refers to the symmetry of the frequency distribution. A measure of skewness is obtained as making use of the second and their moments about the mean. It is defined as,

Eastern blotting is a technique to detect protein post translational modifications (PTM) and is an extension of the biochemical technique of western blotting. There are multiple versions of the method, but in general proteins or lipids are blotted from one or two-dimensional SDS-PAGE gel on to a PVDF or nitrocellulose membrane are analyzed for post-translational protein modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the PTM and the probe, distinguishing them from a standard Far-western blot. The most common application is lectin blotting, however, a variety of other probes can be used for detecting different PTMs.

History and Multiple Definitions

Definition of the term Eastern blotting is somewhat confused due to multiple sets of authors dubbing a new method as Eastern blotting, or a derivative therof. All of the definitions are a derivative of the technique of Western blotting developed by Towbin in 1979. The current definitions are summarized below in order of the first use of the name, however all are based on some earlier works. In some cases, the technique had been in practice for some time before the introduction of the term.

Practical have a significant role in the study of any theoretical subject. Whatever a student learns in theory, he verifies it by actual doing with his own hands.  Thus, these reduce any doubts and misbelieve from his mind. These verifications by actually doing the things are performed in the laboratories.  A laboratory is a place with the basic and essential equipments and adequate facilities for students and teachers to perform and observe experiments.  Plants will continue to provide novel products as well as chemical models for new drugs in the coming centuries.  The arrival of chemical analysis and the characterization of molecular structures have helped in precisely identifying these plants and correlating them with their activity under controlled experimentation.

Bioinformatics can be defined as “the collection, archiving, organization and interpretation of biological data” (Orengo et al., 2003). By extension, structural bioinformatics deals with the collection, archiving, organization and interpretation of 3-D structural data of bio macromolecules. Structural bioinformatics emerged with the first crystal structures of haemoglobin and myoglobin. These structures were analyzed and contributed significantly to our understanding of the principles of life at a molecular level. A repository of all publicly accessible protein crystal structures, the Protein Data Bank (PDB) was established by Walter Hamilton et al., at Brookhaven National Laboratory in 1971. Until the late 1980s, structural biologists often knew all the available structures of bio macromolecules and much of the analysis, such as structure comparison and hydrogen-bonding analysis was done manually without much computer intervention. As the number of available structures increased, so did the need for software tools for structure analysis. Structural bioinformatics encompasses a wide variety of applications, including structure visualization, validation, classification, structure-based function prediction, identification of targets for structural genomics projects and prediction of the structures of individual bio macromolecules and complexes as well the binding modes of small molecules inside proteins of pharmaceutical or biotechnological interestStructural and theoretical analyses of proteins are central to the understanding of complex molecular mechanisms and are fundamental to the drug discovery process.

Biodiversity conservation and sustainable use has, for many people, become associated mainly with issues related to plants and wild animals. Although much less discussed, the loss of farm animal genetic resources may well be much more serious than in crops because the gene pool is smaller and very few wild relatives remain. The fact that 32% of livestock breeds worldwide are at risk of becoming extinct and that the rate of extinction continues to accelerate (FAO, 2000) is thus a serious cause for concern. Animals of different characteristics and hence outputs suit differing local community needs.

The union territory of A & N Islands constitutes the farthest and the remotest part of Indian union. The islands are situated in the Bay of Bengal surrounded by sea. The neighboring countries are Burma in north east and Singapore, Malaysia and Indonesia in south east. The Union territory of A & N Islands is situated between 60 and 140 north latitude and 920 and 940 east longitude forming a broken row of continuous islands from. North to South direction. They can be divided into two groups namely Andaman and Nicobar groups. The dreaded 100 channel which is about 145 Kms wide and 400 fathoms deep, separates the two groups

Among several respiratory diseases affecting man, bronchial asthma is the most common disabling syndrome, nearly 7 to 10 per cent of the world population suffer from bronchial asthma, which is a chronic illness involving the respiratory system in which the airway occasionally constricts, becomes inflamed, and is lined with excessive amounts of mucus, often in response to one or more triggers. These episodes may be triggered by such things as exposure to an environmental stimulant (or allergen), cold air, warm air, moist air, exercise or exertion, or emotional stress. In children, the most common triggers are viral illnesses such as those that cause the common cold. A variety of exogenous determinants of asthma were also been, some of which are air pollution, tobacco smoking, diet, occupation and respiratory infection, these pollutants may serve as inciters or triggers of asthmatic reaction in airways that are already hyper responsive, they may exert direct toxic influence on the respiratory epithelium or may augment or modify immune responses to inhaled antigens. During an asthma episode, inflamed airways react to environmental triggers such as smoke, dust, or pollen. The airways narrow and produce excess mucus, making it difficult to breathe. In essence, asthma is the result of an immune response in the bronchial airways. As according to the World Health Organization over 80% of the people in developing countries depend upon traditional medicine for their primary health care. Medicinal plants are considered as source of various alkaloids and other chemical substances essential for mankind.

There aremany different points of view aboutthe nanotechnology. These differences start with the definition of nanotechnology. Some define it as any activity that involves manipulating materials between one nanometer and 100 nanometers. However the original definition of nanotechnology involved building machines at the molecular scale and involves the manipulation of materials on an atomic (about two-tenths of a nanometer) scale.

The debate continues with varying opinions about exactly what nanotechnology can achieve. Some researchers believe nanotechnology can be used to significantly extend the human lifespan or produce replicator-like devices that can create almost anything from simple raw materials. Others see nanotechnology only as a tool to help us do what we do now, but faster or better.

The third major area of debate concerns the timeframe of nanotechnology-related advances. Will nanotechnology have a significant impact on our day-to-day lives in a decade or two, or will many of these promised advances take considerably longer to become realities?

Finally, all the opinions about what nanotechnology can help us achieve echo with ethical challenges. If nanotechnology helps us to increase ourlifespans orproduce manufactured goodsfrom inexpensive raw materials, what is the moral imperative about making such technology available to all? Is there sufficient understanding or regulation of nanotech based materials to minimize possible harm to us or ourenvironment? Only timewill tell how nanotechnology will affect our lives.

After the DNA is run on the gel, the polymorphic bands or the bands cosegregating with a target trait are tagged. The bands identification is either done by calculating their relative mobilities (Rm / Rf values in case of isoenzymes) or by calculating their molecular weights (in case of proteins and DNA).

There are several methods to calculate molecular weights of DNA bands on the gel. Graphical approach is one of them. The approaches include (1) use of computer software to generate molecular weights and (2) running unknown DNA fragments with the fragments of known molecular weights (standard markers available from private companies, see examples in the figure). An example is given here (see figure) to illustrate the method to calculate molecular weights of unknown DNA fragments. This method is usually called as Graphical Approach.

A fundamental problem facing researchers today is how to make effective use of available wealth of online background domain knowledge to improve their understanding of complex biological system. Effective use of such online background domain knowledge plays a crucial role in all stages of integrated biological studies.

In the past years, research in molecular biology and molecular medicine has accumulated enormous amounts of data. This includes genomic sequences gathered by the Human Genome Project, gene expression data from micro array experiments, protein identification and quantification data from proteomics experiments, and SNP data from high-throughput SNP arrays. However, the understanding of the biological processes underlying these data lags behind. There is a strong interest in employing methods of knowledge discovery and data mining to generate models of biological systems. Mining biological databases put forward challenges which knowledge discovery and data mining have to address in the future. Important background knowledge in bioinformatics is often contained in documents, such as scientific publications or database annotations. Analyzing data from biological databases often requires the consideration of data from multiple relations rather than from one single table. Recently, approaches (such as propositionalization algorithms) are being studied that utilize multi-relational data and yet meet the efficiency requirements of large-scale data mining problems.

Sterilization Techniques

Sterilization: All the materials, e.g., vessels, instruments, medium, explants, etc., used in culture work must be freed from microbes. This can be achieved by one of the following approaches:

Algae refer to an assemblage of polyphyletic organism that conducts oxygen evolving photosynthesis other than land plants. They are distributed widely throughout India in the sea, in fresh water and in moist situations on land. Algae also form an important component of biodiversity of Andaman and Nicobar Group of Islands. Database for Algae resources of Andaman and Nicobar Islands are created with an aim of providing an information pool to the researchers working in fields related to fresh water and marine water algae in Andaman and Nicobar Group of Islands. About 313 fresh water algal species belonging to 15 families and 57 marine algal species belonging to 18 families has included in this database among which 40 species has been found to be first time reported in India. Algae have a wide application in a variety of Industries such as Food, Fodder, and Fertilizer etc. They are also important sources of Protein, Iodine, Vitamins, Minerals and Substances of Antibiotic Nature.

Micropropagation /Clonal propagation

Micropropagation or clonal propagation is the growing of plants from meristematic tissue or somatic cells of superior plants on nutrient suitable media under controlled aseptic physical conditions. Sexually propagated plants (through generation of seeds) demonstrate a high amount of heterogeneity since their seed progenies are not true to type whereas asexual reproduction (by multiplication of vegetative parts) gives rise to genetically identical copies of parent plant. Thus, it permits perpetuation of the parental characters of the cultivars among the plants resulting from micropropagation. Micropropagation proves useful for propagation of: