eChapters

13.1. Introduction

Water is chemically benign. However, its interference in free gas exchange in soil becomes detrimental to crop growth when soils are subjected to waterlogging or plants are submerged with water.Waterlogging of soil refers to a soil condition when the soil attains a moisture regime to such an extent that oxygen is not available in the pore space in enough quantity for plant roots to respire adequately. On the contrary, detrimental gases such as carbon dioxide, methane, hydrogen sulphide, ethylene etc. accumulate in the root zone and adversely influence the growth of plants. Thus, anaerobic condition prevails in waterlogged soil causing depletion of oxygen and increase of CO₂ in root zone, which severely affects aerobic micro-organisms while harmful organisms proliferate and restrict the plant growth. Waterlogging leads to nutrient deficiencies or nutrient toxicities, root tip decomposition and eventually, reduced growth or death of the plant. Physical, chemical and biological activities in the soil are also disturbed due to low temperature. However, this is important to note that plants differ among themselves in their demand for oxygen. Even oxygen demand for same plants differs with its growth stage. Therefore, it is well understood that there is no universal level of soil oxygen that can identify waterlogged conditions for all plants. Mostly waterlogging prevails in the land due to non-permeability of the soil material, the presence of an impervious layer in soil horizon, or a high-water table.

12.1. Introduction

All soils invariably contain soluble salts. However, physico-chemical and biological soil properties start degrading when excessive amount of salts are accumulated in soil, particularly in rhizosphere, due to several anthropogenic activities and environmental conditions, and crop production is severely affected. Then we refer those soils as salt-affected soil.

The need of intensive agriculture to keep pace with the increasing world population for their food requirement necessitates to bring more agricultural land area under irrigation. However, irrigation system without taking enough drainage provision and poor water quality and its poor management may make the situation worst due to the development of salt-affected soils that ensue unproductive soil and low crop yield. Crops grown on salt-affected soils suffer from high osmotic stress, nutritional disorders due to nutrient (N, Ca, K, P, Fe, Zn) deficiency, specific ion toxicities, poor soil physical conditions and as a consequence, a reduced crop productivity.

Although it is considered that the rocks and minerals on weathering lead to salt accumulation in the soil, but the salt-affected soils are not truly originated from the accumulation of such salts in situ. On the contrary, occurrence of salt-affected soils is due to receiving salts that come from other locations with water. Sea water inundation in coastal belt, irrigation with poor quality water including saline groundwater, poor drainage system, irrigation adopting unscientific ways leading to waterlogging, shallow water table etc. contribute heavily toward development of salt-affected soils. Salt-affected soils may occur under almost all climatic conditions. Their distribution, however, is relatively more extensive in the arid and semi-arid regions compared to the humid regions because of poor precipitation to leach soluble salt adequately. Since evapotranspiration greatly exceeds precipitation in these climatic conditions, the soluble salts move upward with water due to capillary action and are left behind on the surface soil and the rhizosphere, once water is evaporated. The commanding ions of accumulated salts in affected soil are sodium, potassium, magnesium, calcium, chlorides, sulphates, carbonates and bicarbonates. The nature and properties of salt-affected soils are so diverse that they require specific approaches for their reclamation and management. Therefore, it is necessary to know their origin, formation, nature, chemistry and properties in detail to classify them into groups for their proper management to maintain sustainable agriculture.

11.1. What is acid soil?

Acid soils refer to those soils that are characterized with the presence of higher concentration of H⁺ in soil solution and at exchangeable sites or characterized with its high proton donating capacity such as aluminium (Al³⁺) on hydrolysis gives rise to proton. Both H⁺ and Al³⁺ are responsible to contribute soil acidity. Al³⁺ causes soil acidity indirectly by generating H⁺ through its hydrolysis, while H⁺ contributes directly. Therefore, due to more abundance of H⁺, they exhibit low soil pH and low base saturation (Ca²⁺, Mg²⁺, Na⁺ and K⁺). Such soil is considered as problematic soil because plant growth is affected adversely under acidic conditions and they suffer root injuries when grown in acid soils. Besides, soil acidity interferes in the soil nutrients availability to plants, making them either deficient to plants (such as phosphorus, calcium, magnesium, molybdenum) or toxic to plants for their more availability. Under acidic condition, Al, Mn and Fe are found in more soluble form. Thus, they are taken up by the plant in such a higher level that they become toxic to plants. Phosphorus becomes immobile due to the formation of insoluble compounds with aluminium and iron under acidic conditions. Ca, Mg and Mo also become unavailable in acid soil and show their deficiency symptoms. Soil acidity also affects soil microbial growth and thus, in turn, affects the nutrient dynamics in soil. Soil acidification is induced heavily under intensive agricultural practices such as increasing leaching due to more irrigation practices, addition of fertilizers, build-up of soil organic matter and many other possible ways.

10.1. Introduction

The soils possessing characteristics that are not suitable and economical for crop growth are termed as Problem soils. These characteristics may be of physical, chemical and biological. For cultivation of crops in these soils, it is necessary to follow specific management interventions and proper reclamation measures. Frequently we come across another term, ’degraded soil’. Degraded soil is also a problem soil. However, there exists a hair-line difference between problem and degraded soil. Problem soils refer to the soils in which the inherent soil characteristics, obtained from its genesis, pose problems for their optimal use, while degraded soils are developed due to anthropogenic activities by unwise management interventions.

10.2. Types of problem

Mainly there are four types of problem that may exist in soils: physical problems, chemical problems, biological problems and nutritional problems as a result of physical, chemical and biological constraints in soils.

9.1. Introduction

Soil biology is a branch of science that studies the soil inhabiting organisms in relation to their ecology, functions, behaviour and impact on various soil processes and plant growth. Soil fertility is directly dependant on humus content as well as the presence of microorganisms in soil, although surface soil does not contain more than 0.5% by weight of living organisms. However, such a small portion of soil constituent as compared to other organic and inorganic constituents plays a significant and major role in transformation of plant nutrients from their unavailable to available form from organic wastes, soils and fertilizers. They have a direct role in improving soil tilth, providing an appropriate environment for root growth, decomposing organic residues to form humus and in turn releasing nutrients for plants use, releasing many plant growth promoters, fixing inert atmospheric nitrogen in plant usable forms and they promote many other important processes without which plants cannot survive. Absence of living organisms in soil leads to its infertility. An understanding of microbial processes in soil environment, keeping in view of soil nutrient status, carbon sequestration, soil health and quality, overall sustainability in agriculture and also global warming in respect of greenhouse gas emissions (nitrous oxide and methane) through agricultural practices, is very important in regulating nutrient release at different plant growth stages. If we are in a state to understand how various biological processes influence various changes in soil environment or in the opposite way that how different soil conditions alter the soil biota in number as well as in their functions and diversity, it is easier to implement soil management practices that promote to gain more benefits.

Microorganisms are ubiquitous in nature. Possessing a diverse habitat, they are found everywhere in soil, air and water at a varied temperature and pH. One may find the highest genetic diversity of life within the domain of microorganisms. But unfortunately very few are known to us, so far only 1% of the microbial species on the earth are culturable. However, it is believed that soil of one gram may contain hundreds of millions to billions of microbes which may represent several thousand microbial species. In respect of the population, bacteria top the list followed by actinomycetes, fungi, algae and protozoa in accordance with a decreasing numerical order. Out of three important soil fertility components viz. physical, chemical and biological; the biological component is least understood due to its wide complexity.

8.1. What is an ion exchange reaction and its application in soil system

Ion exchange is a reversible chemical reaction between two substances, either between two electrolytes or between an electrolyte solution and a solid complex as well as between two solid phases (if in close contact with each other), where ions of equal charge may be interchanged in equivalent proportion.

Organic colloids and inorganic micelles (clays) are sites of ion exchange. Soil contains silicate clay minerals which exhibit charges due to isomorphous substitution as well as due to the pH dependant charges for ionisation of hydrogen and hydroxide functional groups. Organic matter present in soil also contributes to the pH dependent charges due to the presence of various active functional groups. Thus, the organic and inorganic colloids present in soil bear positive or negative charges. Electric charge on soil particles is neutralized by equivalent amount of oppositely charged exchangeable counter ions, held by coulomb forces and van der Waals forces. Ca⁺², Mg⁺², H⁺, K⁺, Na⁺, NH₄ ⁺, Al⁺³, Fe⁺³, Fe⁺², Mn⁺², etc. are the common cations present in soil where Ca⁺² serves as the dominant ion. However, Na⁺ and Al(OH)₂ ⁺ are the dominant cations found in the exchangeable phase in strong alkali and strong acid soils, respectively. SO₄ ^- 2, Cl^-, HCO₃ ^-1, NO₃ ^- etc. are the most common anions found in soil to neutralize positively charged soil colloids.

7.1. Introduction

Like all other living organisms such as humans, animals, and microorganisms, plants also need elemental nutrients for their growth and development. They assemble the nutrients from soil, water and air. However, to obtain economical crop yield, nutrients are also supplied to the crops from external sources such as chemical fertilizers and manures and using biofertilizers. It is the present-day need to handle the soil fertility status in a more realistic and careful way to obtain maximum crop harvest. Under poor management practices, soil may become infertile and loose its importance in agricultural use. In modern days, huge food demand for the burgeoning human population does not permit to show luxury to use only fertile soil. Moderately fertile soil or even degraded soil should also be managed properly so that they may be used to grow crops. For this, plant nutrients status of the soil plays a significant role along with other soil properties. For sustainable soil fertility management, parameters such as crop to be raised, its nutrient demand at various growth stages, current nutrient status of the soil, amount of nutrient application based on the soil testing results etc are important to be considered. Balanced nutrition to the plants is the prerequisite to obtain maximum benefit. If nutrients supplied to the plants are low in quantity than their requirement, crop production is lowered down. Similarly, if the nutrients are provided in more amount in the soil or unbalanced way through fertilizer or manure applications, total crop yield is suffered. It is a very common example that excess of phosphorus leads to zinc deficiency in most crop plants and it has been observed that excess N is responsible for potassium starvation in potato. Again, excess of iron, manganese, zinc, copper shows plant toxicity. Addition of nutrients to soils in excess also causes environmental pollution when the excess nutrients are lost from soil by leaching, surface run-off and volatilization. Unscientific way of soil fertilization leads to increasing cost of cultivation and imparts adverse effect to the environment instead of doing any good. Therefore, one should have a clear knowledge on soil fertility to obtain a sustainable productivity.

6.1. What is colloid and colloidal system?

A colloidal particle may be defined as a substance which is microscopically dispersed evenly throughout another phase and is possessing a size of 10^-7 to 10^-3 mm and representing a larger surface area per unit mass. Thus, a colloidal system refers to a two-phase system in which small particles of one phase which might be of solid, liquid or gas is suspended evenly in the second phase of solid, liquid or gaseous material. These colloidal systems may be of solid in liquid or solid in gas or liquid in liquid or liquid in gas. Unique examples of liquid in liquid colloidal system includes the fat droplets dispersed in water of milk. When you put floor disinfectant liquid in water, it forms a white emulsion. Thus, emulsion becomes a colloidal system of liquid in liquid. Likewise, in fog, water droplets are suspended in gaseous air. So, fog is an example of a colloidal system, constituting of liquid dispersed in gas. When we refer smoke, it contains carbon particles (solid) suspended in air (colloidal system of solid and gas). However, smog is composed of smoke and fog. In case of soil colloidal system, soil colloids, which are of tiny particles (<0.001 mm size) and may be of organic and inorganic nature, are suspended in soil solution (water) and referred to as sol. Colloidal chemistry, which involves in the surface reactivity and charge characteristics of colloids, plays a pivotal role in total soil chemistry.

5.1. What is soil organic matter?

Plant and animal residues which are not yet added to the soil are considered as organic materials. However, once they are added to soil, they become soil organic matter. Therefore, organic materials contributing to the soil matrix are referred as Soil Organic Matter (SOM). Sometimes SOM is also used to highlight only the non-living organic materials produced by living organisms. But SOM should be regarded as a broad term that includes biomass, partially decayed residue, and highly decomposed and transformed organic substances, humus. Agricultural soil contains nearly 1-5% of SOM, which is regarded as least amount in soil as compared to other three soil components, minerals (45%), air (25%) and water (25%). However, it contributes enormously to soil productivity. Soil productivity and fertility exhibit direct positive relationship with SOM content in soil. Plants and animals are considered as primary and secondary sources of soil organic matter, respectively. Plant residues and application of organic manures e.g., farmyard manure, compost or green manuring help to build up organic matter in soil and thus, enrich the soil with organic carbon and other plant nutrients. Under natural conditions, roots of grasses in grasslands, the leaves including shoot portion of the large trees in forest lands and after harvesting the crop remnants in agricultural field provide enough incorporation of organic materials in soil. SOM plays a vital role to maintain sustainability in agricultural production through producing healthy soils and thus, healthy crops.

4.1. What is pH?

pH is used to classify substance as acidic, neutral and alkaline, based on the prevalence of free H⁺ ions. Water molecules on autoionization dissociate to produce H⁺ and OH^- in equal number, although degree of dissociation is very small because of its stability (about one molecule in 10 million is ionized at any one time). H⁺ ions later attached to un-ionized water molecule to give rise hydronium ions, (H₃O)⁺

3.1. What is weathering?

Weathering may be defined as the transformation process of solid rocks, involving the disintegration and decomposition of rocks and minerals, into soil.

There are different types of weathering: physical, chemical and biological.

3.1.1. Physical weathering

In physical weathering the chemical composition of the weathered products remains same as that of the parental rocks. It is involved in fragmentation of rocks into comparatively smaller sizes. It does not transform the parental rock into a newer one that may differ from the parental rock in chemical composition. The process is totally physical in nature. But this is also true that physical weathering encourages chemical weathering, and both may be carried out simultaneously because physical weathering promotes more surface area of parental rocks for more interaction with agents of chemical weathering.

2.1. Minerals

Primarily, minerals are usually inorganic substances possessing a definite chemical composition and a definite molecular arrangement which is usually expressed in geometric form. The mineral matter is made up of various elements, combined to form compounds. Usually, minerals consist of more than one constituent elements. Quartz contains two elements, silicon and oxygen. Other minerals may contain many elements, such as amphibole consists of several elements that include sodium, calcium, magnesium, iron, aluminium, silicon, and oxygen. Some elements exist as such without forming compounds. They contain exceptionally one element. Graphite (C), diamond (C), and Sulphur (S). Minerals are naturally occurring crystalline solid where the atoms are arranged in a regular three-dimensional array. A crystal is a solid, formed by a repeating three-dimensional pattern of atoms, ions, or molecules & possessing fixed distances between the different parts. Minerals that do not grow in these regular or crystalline patterns are called amorphous solids. When we talk about ‘naturally occurring’, we exclude the laboratory-synthesized crystalline solids. Glass and amber are also naturally occurring solid, but they are not crystalline and thus, they are not considered as minerals, rather they are referred as amorphous solids.

1.1. What is Earth?

The Earth is a layered body consisting of three parts: core, mantle, and crust. The radius of the Earth is nearly 6370 km of which crust, mantle and core share a depth of 40, 2850 and 3480 km, respectively (Figure 1.1). Exterior of the Earth contains the outer lithosphere which is surrounded (70% of surface) by water (hydrosphere). Solid and liquid sphere are surrounded by gas (atmosphere). The atmosphere is of 320 km above the lithosphere / hydrosphere. It is believed that there is a progressive increase in temperature with depth, if one goes to the core from the Earth crust. There is a rise of approximately about 1°F for every 64 feet depth on an average. Therefore, one could imagine that the interior of the earth is in molten condition.

At this moment, the majority of energy is obtained from fossil fuels and requires switching over to alternative energy sources is the need of the hour. Fossil fuels are nonrenewable energy source and also have seriously negative impacts on the environment. The use of fossil fuels cause excessive global climate change because emissions of greenhouse pollutants and the formation of compounds CO_x, NO_x, SO_x, C_xH_y, ash and other organic compounds that are released into the atmosphere. The increase in greenhouse gas emission will result in global warming, climate change, environmental degradation and health problems.

Biofuel, any fuel that is derived from biomass - that is, plant or algae material or animal waste. Since such feedstock material can be replenished readily, biofuel is considered to be a source of renewable energy, unlike fossil fuels such as petroleum, coal, and natural gas.

Bacterial biofertilizers are supplied as carrier based inoculants. Peat or lignite is used as carrier material. Carrier based bacterial inoculants are applied by the following methods.

1. Seed treatment or seed inoculation.
2. Seedling root dip and
3. Main field application

Seed Treatment

One packet of the inoculant (200 g) 7is mixed with 200 ml of rice kanji to make a slurry. The seeds required for an acre are mixed in the slurry so as to have a uniform coating of the inoculant over the seeds and then shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is sufficient to treat 10 kg of seeds.

Biofertilizers are one of the important components of organic farming and sustainable agriculture. Awareness among farmers is increasing on the biological fertilizers. But, the success of biofertilizer inoculation depends on the quality of the inoculant used. Because farmers are unable to judge the quality of the inoculants at the time of purchase or even at use. The inoculant producers are negligent to institute quality control programmes. Unfortunately much of the inoculant produced in the world today is relatively of poor quality. In such situation maintaining high quality inoculant products depend on the internal implementation of effective quality control systems by manufacturer. A major goal of a quality standard is to control the quality of inoculant to be used in any country.

Mass Production of Bacterial Inoculants

Strain Selection and Improvement

In biofertilizer technology, strain selection occupies the foremost criteria, so as to explore its biological potentialities for the beneficiary crop. The selected strain used in biofertilizer production must be efficient to grow fast besides retain desirable potentialities of biological activities, which should be present in desired high numbers and must be stable. The strain must be improved periodically so as to sustain the productivity.

Chemical pesticides though extremely effective in controlling pest population suffer from disadvantages. They have a broad toxic range and potentially toxic to non target organisms. Other constraints that have emerged with the use of chemical pesticides such as development of resistance and resurgence in insects, residual toxicity, secondary outbreak of minor insect pests, etc., In this juncture, the biopesticides have proven to be effective tool in managing the devastating insect pests of various crops.

The biopesticides are types of pesticides derived from natural materials such as microorganisms, plants, animals and certain minerals. They are living organisms or their products or byproducts, which can be used for the management of pests that are injurious to crop plants. Biopesticides have a vital role in crop protection, although most commonly in combination with other tools including chemical pesticides as part of integrated pest management (IPM). Microorganisms help to regulate the abundance of many pests or potential pests without any cost to society. Microbes can be increased artificially and thereby achieve faster, higher and more consistent control of the target pests that will occur naturally. Insect pathogens that are transmissible in nature from one host to another offer an important bonus effect that chemical insecticides do not have. A pathogen will kill pests exposed and many continue killing them by natural transmission from one individual host to another through subsequent host generation.

Biopesticides generally have several advantages compared to conventional pesticides. While chemical pesticides are responsible for extensive pollution of the environment, a serious health hazard due to the presence of their residues in food and development of resistance in targeted insect pest populations, biopesticides, in contrast, are inherently less toxic to humans and the environment, do not leave harmful residues, and are usually more specific to target pests. The agents employed as biopesticides, include parasites, predetors and disease causing fungi, bacteria and viruses, which are the natural enemies of pests. Utilization of naturally occurring parasites, predators and pathogens for pest control is a classical biological control. These bio agents can be conserved, preserved and multiplied under laboratory condition for field release. Once these bio-agents are introduced in the field to build their population considerably, they are capable of bringing down the targeted pest population below economic threshold level (ETL).

For many soil borne diseases, effective and economic control methods are not available. Several soil borne pathogens have developed resistance to many fungicides. The beneficial microbes are also affected by fungicides. Many fungicides pose health hazards by polluting food and water and cause direct toxicity of heavy metals and carcinogenic risk. In the context of growing worldwide awareness of environmental pollution and development of fungicide resistant strains of pathogens, biological method of suppression of disease causing organisms have been recognized as the safest approach for plant disease management.

Biological control of plant diseases is the suppression of populations of plant pathogens by living organisms. Amongst beneficial microorganisms, isolates can be selected which are highly effective against pathogens and can be multiplied on artificial media. Application of such selected and mass produced antagonists in high densities once or several times during a growing season is called “augmentative biological control”.

Mycorrhizal symbiosis is the most prevalent and important mutualistic association in plant kingdom. ‘Mycorrhiza’- the word means fungus - root, this association was reported by A.B.Frank in the year 1885. Nearly 95% of plant species form this mycorrhizal association. This serves as critical linkage between plant roots and soil and characterized by the movement of plant produced carbon to fungus and fungal acquired nutrients to plants. Through its hyphae this fungus absorbs the available P from soil and transports directly to root cortex. Mycorrhizal associations vary widely in structure and function. There are seven types of mycorrhizae (Table 1). These are endo (arbuscular), ecto, ectendo, arbutoid, monotropoid, ericoid, and orchidaceous mycorrhizae. Among them, endomycorrhizae and ectomycorrhizae are the most abundant and widespread