eChapters

15.1 Law of Mass Action

Gulberg and Waage, the two Norwegian chemists in the year 1864 stated the law of mass action in this form: ‘The velocity of any chemical reaction at any instant at a given temperature is proportional to the active mass of each of the reactants at that instant present in the system, the active mass in a homogeneous system is defined as the number of gram molecule of the substance present per unit volume i.e., the molar volume’.

Soil sampling is an integral and essential component of soil fertility evaluation and nutrient management research. The effectiveness of soil sampling is a prerequisite for soil testing to achieve its goals for efficient nutrient management and plant nutrition for improved yield and quality. The underlying basis for soil sampling is that a soil sample taken represents the “population” which may be a plot, field or a watershed. It further implies that nutrient status of the representative soil sample(s) determined in a laboratory would reflect nutrient status of a plot, field or watershed and is of interest for correcting nutrient disorders in the field or watershed.

The most important factor that influences the effectiveness of soil sampling is soil heterogeneity. However, in a relatively homogenous group of fields or plots, a small number of samples may be sufficient to represent the population to a more heterogenous group of fields that would require more number of samples to represent the soil population. Whenever, soil samples to be taken in field following points needs to be kept in mind.

Rerd. L.L.Langstroth (Father of modern bee keeping) discovered movable frame hive in 1851.

In 1882, beekeeping was introduced in India.

In 1911, Rerd. Newton introduced beekeeping to South India.

Nano-bioinoculants are poised to transform Indian agriculture with solutions tailored to its diverse agro-climatic regions, prevailing smallholder farm management systems, and environmental sustainability. The chapter explores the progress, present research scenario, and future scope of nano-bioinoculants in India. These have been built upon early nanotechnology explorations catalyzed by government initiatives like the Nano-Mission and augmenting research infrastructure, with ongoing research being marked by crop-based studies of Indian crops and utilizing the immense diversity of indigenous microorganisms in cutting-edge nanomaterials. Through controlling the complex plant-microbe interactions to biotic and abiotic stresses, the reaction of nano bioinoculants is being performed using advanced omics-based studies. Great progress is being made in the development of smart delivery systems for precision agriculture, in conjunction with novel formulation methods such as layer-by-layer assembly and stimulus-response release. The use of sensors, drones, and AI-based systems opens up possibilities for targeted application and real-time monitoring. Though commercialization is picking up speed via new start-ups and businesses, costeffectiveness issues, farmer awareness, and an immature regulatory system continue to be a challenge. Looking at the future, promising directions for intelligent farming highlight the revolutionary significance of such beneficial microbes in shaping a more durable, productive, and sustainable future for agriculture in India.

Composting is organic matter that has been decomposed and recycled as fertilizer and soil conditioner. It is a natural process of decomposition and recycling of organic material into humus rich soil amendment.

At the simplest level, the process of composting requires making a heap of organic material sprinkled with water which break down to humus after a period of weeks or months. However, modern bio composting is a multi-step, closely monitored process with measured inputs of water, air and carbon and nitrogen rich (in proper C:N ratio) materials.

Bio Control Agents explains how natural enemies can suppress agricultural pests and diseases through predators, parasitoids, and pathogens. It traces the historical development of biological control from traditional practices to major scientific successes and institutional development in India. The chapter describes introduction, augmentation, and conservation strategies, followed by detailed accounts of predators, parasitoids, and pathogenic microorganisms. Important bioagents, their characteristics, mechanisms, field applications, and mass-production methods are discussed, including Trichogramma, Chrysoperla, Cryptolaemus, Beauveria, Metarhizium, Trichoderma, Bacillus, and viral agents. The chapter provides a practical foundation for integrating biological control into environmentally responsible crop protection programs. across diverse farming environments and practical field implementation practically.

To many Bio Fertilizer is still an unclear term. Often they are identified as urban and rural organic wastes, plant extracts, organic components etc. However, Bio Fertilizer is mostly commonly referred to the use of soil micro organisms to increase the uptake and availability of plant nutrients.

Bio Fertilizer have gained a good deal of momentum and the industry after a slow start is now gaining more and more importance globally. The major reasons for this are that they:

All organisms require the six elements CHNOPS in sizable quantities, and that these minerals may occur in different forms. Depending on whether the local environment is aerobic or anaerobic, the exact chemical form of any of these elements may change quickly and drastically. Example: : If the soil becomes waterlogged and anaerobic, sulfate will be converted into hydrogen sulfide and nitrate into nitrogen gas or ammonia (via anaerobic respiration). As the water recedes and the soil becomes aerobic again, the ammonia is oxidized back to nitrate in stages. All of this (and many other processes) occur largely because of the activities of soil microbes. Without these microbes, life as we know it would not occur because necessary elements would remain tied up in unusable chemical forms and gradually be removed by sedimentation. To maintain the level of these components it is essential that they undergo a regular process of recycling. Different microorganisms bring about this process of recycling through various transformations.

Carbon Cycle

The most important element in the biological realm and substance that serve as the cornerstone of the cell structure is carbon. It constituents about 40-50% of all living organisms, yet the ultimate source is the CO₂ that exists in a perennially short supply, only 0.03% of the earth’s atmosphere, which undergoes a cyclic change from an oxidized to reduced state. The concentration of carbon in living matter (18%) is almost 100 times greater than its concentration in the earth (0.19%). Hence, living things extract carbon from their non-living environment. For life to continue, this carbon must be recycled. Carbon exists in the nonliving environment as carbon dioxide (CO₂) in the atmosphere and dissolved in water (forming HCO₃") carbonate rocks (limestone and coral = CaCO₃) deposits of coal, petroleum, and natural gas derived from once-living things dead organic matter, e.g., humus in the soil. Carbon enters the biotic world through the action of autotrophs: primarily photoautotrophs, like plants and algae, that use the energy of light to convert carbon dioxide to organic matter through photosynthesis and to a small extent, chemoautotrophs - bacteria and Archaeans that do the same but use the energy derived from the oxidation of molecules in their substrate.

Reducing the input costs in farming, especially agrochemicals and improving the yields of farm products in environmentally sustainable way, is one of the most important challenges. World over, the understanding of alternative eco-friendly inputs and intelligent integration of such products with the farming practices are the areas which need to be emphasized, understood and demonstrated. Our experience of working with farmers in different regions has convinced us that they all are looking for alternative, eco-friendly and cost effective inputs and the only way to convince them is practical demonstrations. 
Eco safe farming is a global challenge and needs combined efforts of NGO_s and farmers bodies, private and public sector industries and scientists in the institutions and universities. There are many challenges and many success stories and some typical once are listed here.

At an Agri Summit, I had a very difficult task to chair a session on “Sustainable Future”, with two well-known but extremely opposite personalities. On my left was a globally well-known environment activist talking about all the evils of chemical fertilizers and chemical pesticides. On my right was the President of Pesticide Association with equally strong views about how the chemical pesticides have greatly helped in feeding the hungry world. My task was further made difficult by a large lobby of green activists in the audience and also a number of seasoned industrialists with opposite views. Such a scenario is becoming increasingly common.

Bio-Inputs introduces the concept, scope, and functional diversity of biological inputs used in sustainable agriculture and natural farming. The chapter classifies bio-inputs by core components, including microorganisms, biological extracts, and biomolecules, and then by functions such as bio-fertilizers, bio-stimulants, biomejorators, and biological control agents. It also presents microbial and biological inputs within Integrated Pest Management, emphasizing biopesticides, biofungicides, and plant-defense elicitors. Examples such as Rhizobium, Trichoderma, Bacillus, Pseudomonas, seaweed extracts, vermiwash, neem extracts, and oligosaccharins illustrate how biological resources can improve nutrient availability, plant growth, crop protection, stress tolerance, and ecological sustainability. in contemporary agricultural practice. for sustainable crop production.

A bioassay, also known as a biological assay, is a scientific experiment used to quantify the impact of a substance on a living organism. According to Finney (1952), bioassay is defined as measurement of potency of any stimulus (physical, chemical, biological, physiological) by means of reactions which it produces in living matter. It is an essential procedure in insecticide development and the assessment of environmental pollutants, as well as the evaluation of the effects of interventions viz. pesticides and herbicides on the environment. In the field of biostatistics, a bioassay refers to a set of techniques used to determine the quantity or intensity of an agent or stimulus based on the response of the subject. Typically, many methods are employed to assess the strength or effectiveness of an insecticide by examining its impact on living organisms.

An insecticide is a pesticide used to kill or eliminate insect pests in agriculture, households, and industries. Judicious use of insecticides may be a factor in the increase of agricultural productivity, but by their nature of having high toxicity to non target organisms and capability to develop resistance through widespread use, most insecticides have high potential to significantly affect and alter ecosystems. Many are toxic to humans and animals (both domestic and wildlife), and can accumulate as concentrates in the food chain and water resources, giving rise to serious environmental contamination and pollution.

Introduction

Soil plays a vital role in fulfilling fundamental human requirements, such as the provision of sustenance and water, while also serving as the primary medium for supporting biodiversity. The sustainability of soil in the 21st century is dependent upon not only the implementation of effective management practices by farmers, foresters, and land-planners, but also on government regulations, policies, marketing strategies, and subsidies (Mishra et al., 2017). The increasing influence of human activities on the Earth’s natural environment has led to significant global challenges concerning the interplay of planetary and public health (J. Huang et al., 2018).

The synthesis of humic substances is the least understood and the most intriguing aspect of humus chemistry. This has been one of the intensely- researched subjects for a long time. Formation of humic substances takes several pathways during the decay of plant and animal remains in soil and the four important ones are shown in Fig. 8.1.

Fig. 8.1: Mechanisms for the formation of soil humic substances

The classical theory by Waksman advocated humic substances as modified lignins (Pathway 1), but a vast majority of current crop of investigators favour a mechanism involving quinones (Pathways 2 and 3). Including sugar-amine condensation pathway (Pathway 4), all the four pathways must be considered as likely mechanisms for the synthesis of humic and fulvic acids in nature. It is absolutely possible that these four pathways may operate in all soils, though not to the same extent or in the same order of importance. 

Bioclimatology examines relationships between climate and living organisms, including crops, livestock and humans. It draws on biology, ecology, meteorology, architecture and physiology to explain how temperature, humidity, radiation and wind influence development, comfort, health and productivity. This chapter introduces bioclimatic indices such as the Temperature-Humidity Index, Humidex, wind chill and thermal-comfort measures. Agricultural applications include crop and livestock suitability, heat-stress assessment, housing design, work planning and development of resilient farming systems. Understanding biological responses to climatic conditions supports appropriate management interventions and helps protect productivity and welfare. Bioclimatic analysis therefore connects atmospheric conditions with practical decisions involving plants, animals and people.

For many soil borne diseases, effective and economic control methods are not available. Several soil borne pathogens have developed resistance to many fungicides. The beneficial microbes are also affected by fungicides. Many fungicides pose health hazards by polluting food and water and cause direct toxicity of heavy metals and carcinogenic risk. In the context of growing worldwide awareness of environmental pollution and development of fungicide resistant strains of pathogens, biological method of suppression of disease causing organisms have been recognized as the safest approach for plant disease management.

Biological control of plant diseases is the suppression of populations of plant pathogens by living organisms. Amongst beneficial microorganisms, isolates can be selected which are highly effective against pathogens and can be multiplied on artificial media. Application of such selected and mass produced antagonists in high densities once or several times during a growing season is called “augmentative biological control”.

Bioconversion refers to the recycling and reprocessing of organic waste materials using the system of biological decomposition or biodegradation into value added products. Composting is one way of conversion of organic waste into manures. It is defined as a method of solid waste management whereby the organic component of the solid waste stream is biologically decomposed under controlled conditions to a state in which it can be handled, stored or applied to the land without adversely affecting the environment.

Composting provides a partial solution to an issue of great concern in many communities. All around the world, landfills are filling up, garbage incineration is becoming increasingly unpopular, and other waste disposal options are becoming ever harder to find. Composting provides a way not only of reducing the amount of waste that needs to be disposed of, but also of converting it into a product that is useful for agriculture. Composting reduces waste, makes us less dependent on landfills, and decreases greenhouse gas emissions. Composting recycles essential nutrients back into the soil and promotes a prolific soil ecosystem.

Introduction

More than a third is covered by drylands in the terrestrial land surface, which have a harsh and vulnerable natural condition (Maier et al., 2014). Due to insufficient water availability, dry areas have a scant population of vascular plants, as well as poor availability of the nutrient turnover with poor availability of the resources as a result of severe temperatures (Adeel, 2008). Biological soil crusts or Biocrusts commonly protects the soil surface in dry environments and it is considered “ecosystem engineers” of arid and semiarid areas, as well as signs of ecosystem health (Briggs and Morgan, 2011; Bowker et al., 2008). They are connected in dryland ecosystem restoration level or degradation (Assouline et al., 2015). When compared to chemical or physical crusts, biocrust is the largest word that explicitly implies that these are the crusts which are dependent on the living organisms’ activity.

Pesticides are the chemical substances that kill pests and herbicides are the chemicals that kill weeds. In the context of soil, pests are fungi, bacteria, insects, worms, and nematodes etc. that cause damage to field crops. Thus, in broad sense pesticides are insecticides, fungicides, bactericides, herbicides and nematicides that are used to control or inhibit plant diseases and insect pests. Although wide-scale application of pesticides and herbicides is an essential part of augmenting crop yields; excessive use of these chemicals leads to the microbial imbalance, environmental pollution and health hazards. An ideal pesticide should have the ability to destroy target pest quickly and should be able to degrade nontoxic substances as quickly as possible.

The ultimate “sink” of the pesticides applied in agriculture is soil. Soil being the storehouse of multitudes of microbes, in quantity and quality, receives the chemicals in various forms and acts as a scavenger of harmful substances. The efficiency and the competence to handle the chemicals vary with the soil and its physical, chemical and biological characteristics.

Biodiversity encompasses the different kinds of life we find in one area— the variety of animals, plants, fungi, and even microorganisms like bacteria that make up our natural world. Each of these species and organisms work together in ecosystems, like an intricate web, to maintain balance and support life. Biodiversity refers to all species and living things on Earth or in a specific ecosystem. Biodiversity brings together the different species and forms of life (animal, plant, entomological and other) and their variability, that is to say, their dynamics of evolution in their ecosystems. Traditionally, there are three levels of biodiversity: genetic diversity, species diversity, and ecosystem diversity. But what does each of these levels really mean.