eChapters

4.1 Introduction
4.1.1 Overview of Genomics in Biotechnology
The genome of an organism serves as the ‘blueprint of life’ (Baeza, 2024) and functions as an information repository (Goldman & Landweber, 2016), containing the necessary information for the synthesis of molecules, cells, and tissues (Abera et al., 2017). A study of the entire collection of genes within an organism’s genome is known as genomics (NIH, 2022; WHO, 2025). Genomics encompasses aspects of genetics, focusing on the comprehensive characterization of an organism’s entire set of genes rather than on individual genes (Baeza, 2024). The investigation of living organisms has undergone a transformation through the application of genomics and related fields, providing unique insights that enhance various applications aimed at improving human life, economic conditions (Kalaitzandonakes et al., 2023), and environmental sustainability (Mudaliar et al., 2023).
According to a genomic analysis, 80% of the 6,000 rare disorders have genetic causes (Brittain et al., 2017). Cancer, a genetic disease, has revealed through DNA sequencing the mutational events and oncogenic drivers (D. Wang et al., 2023). Genomic tests contribute significantly to the prediction and prevention of diseases, including familial hypercholesterolaemia (FH) (Brittain et al., 2017). Agricultural industries are currently confronting multiple challenges, including global changes in climate (Bai et al., 2024), growing populations (Putri et al., 2019), resource depletion (Feng et al., 2023), reduction of cultivable land (Mirzabaev et al., 2023), and the prevalence of pathogens (Hossain & Roslan, 2023). Advances in genomic technologies, such as DNA sequencing and gene editing, have transformed the field. DNA sequencing includes; Nextgeneration sequencing (NGS) (Marudamuthu et al., 2023), Ribonucleic acid sequencing (RNA-seq) (Upton et al., 2023), whereas, gene editing methods includes; Clustered Regularly Interspaced Short Palindromic Repeat- CRISPR associated protein 9 (CRISPR/ Cas9) (Q. Liu et al., 2021), Transcription activator-like effector nucleases (TALENS) Zinc-finger nucleases (ZNF) (Malzahn et al., 2017) and Oligonucleotide directed mutagenesis (ODM) (Ravikiran et al., 2025) as well as doubled haploids (Chen et al., 2024), molecular markers (Kumar et al., 2024) and mapping populations (Temesgen, 2021), offers potential solutions against such agricultural challenges (Hossain & Roslan, 2023). Genome sequencing techniques are applied to various fields of industrial biotechnology such as; strain development or improvement (Salazar-Cerezo et al., 2023), enzyme discovery (Zaparucha et al., 2018) and analysis of microbial community (Hosokawa et al., 2022). The potential to enhance microbial strains through the incorporation, deletion, or alteration of

The biological diversity of each country is a valuable and vulnerable natural resource. Sampling, identifying, and studying biological specimens are among the first steps toward protecting and benefiting from biodiversity. Knowledge about biodiversity and the ability to identify organisms that comes with it are global public goods. That is, this knowledge can create benefits for all countries - benefits that no one country can create by itself.

DNA Barcoding: Documenting Biodiversity with a Gene Sequence

“DNA barcoding” is an exciting new tool for taxonomic research. The DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus belongs. The Consortium for the Barcode of Life (CBOL) is promoting international partnerships that will enable people in all countries to better understand and protect their biodiversity.

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism’s mitochondrial DNA to identify it as belonging to a particular species. It is based on a relatively simple concept: most eukaryote cells contain mitochondria and mitochondrial DNA (mtDNA) has a relatively fast mutation rate, which results in significant variance in mtDNA sequences between species and, in principle, a comparatively small variance within species.

6.1 World Trade Organization (WTO) and Trade-Related Aspects of Intellectual Property Rights (TRIPS)
6.1.1 Introduction
The globalization of trade and the rapid advancement in innovation—particularly in biotechnology—have necessitated the harmonization of intellectual property rights (IPR) across nations. The World Trade Organization (WTO), established in 1995, is the apex multilateral body that regulates international trade among member states. Within its legal framework, the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS) plays a foundational role in setting minimum standards for IPR protection and aligning national laws with global trade norms.
In the realm of biotechnology, TRIPS has profound implications on patentability of life forms, technology transfer, public health, biodiversity, and traditional knowledge.
6.1.2 World Trade Organization (WTO): An Overview
• Established: January 1, 1995
• Headquarters: Geneva, Switzerland
• Membership: 164 members (as of 2024), including India
• Primary Objectives:
• Facilitate free and fair global trade
• Administer trade agreements
• Resolve trade disputes
• Monitor national trade policies
• Promote cooperation with other international organizations (e.g., WIPO, WHO)

Introduction 
Chapter 16, “Global Warming and Food Security” delves into the intricate relationship between global warming and food security. The chapter opens by defining the multifaceted concept of food security, which includes availability, access, utilization, and stability. It emphasizes the critical importance of these components in ensuring a stable and reliable food supply. The chapter then explores various challenges to food security, highlighting the impact of rapid population growth, climate change, land and water scarcity, poverty, inequality, and conflicts. It underscores how these challenges are compounded by global warming, which exacerbates environmental stressors such as rising temperatures, altered weather patterns, and extreme weather events. The implications for agriculture are profound, affecting crop yields, livestock health, and overall food production. The section on the causes and consequences of global warming outlines key phenomena such as risi ng temperatures, changing weather patterns, melting glaciers, and ocean acidification. It illustrates how these factors disrupt agricultural systems and food distribution networks, leading to increased food insecurity. To address these issues, the chapter discusses various strategies for enhancing food security in a changing climate. These include adopting climate-smart agricultural practices, diversifying crop and livestock systems, developing climateresilient crop varieties, and implementing efficient water management strategies. It also highlights the importance of policy and institutional interventions, supportive measures for smallholder farmers, and research and development initiatives. The chapter concludes with a discussion on the need for collaboration and partnerships among various stakeholders, including governments, international organizations, and the private sector, to effectively address the dual challenges of global warming and food security. Global warming, a consequence of human-induced climate change, has emerged as one of the most pressing challenges of our time. As the Earth’s average temperature continues to rise, it brings about far-reaching impacts that extend to various sectors, including food and agriculture. The intricate relationship between global warming and agriculture is of paramount importance, as agricultural systems are both vulnerable to climate change and significant contributors to greenhouse gas emissions. Understanding the impacts of global warming on

After the DNA is run on the gel, the polymorphic bands or the bands cosegregating with a target trait are tagged. The bands identification is either done by calculating their relative mobilities (Rm / Rf values in case of isoenzymes) or by calculating their molecular weights (in case of proteins and DNA).

There are several methods to calculate molecular weights of DNA bands on the gel. Graphical approach is one of them. The approaches include (1) use of computer software to generate molecular weights and (2) running unknown DNA fragments with the fragments of known molecular weights (standard markers available from private companies, see examples in the figure). An example is given here (see figure) to illustrate the method to calculate molecular weights of unknown DNA fragments. This method is usually called as Graphical Approach.

Introduction 
To fight climate change and lessen dependency on fossil fuels, the world urgently needs renewable and environmentally friendly energy sources. Hydrogen has become a promising energy carrier due to its versatility and capacity to generate energy from renewable resources without releasing greenhouse gases into the atmosphere. The world's demand for energy has been steadily increasing as a result of urbanization, economic expansion, and population rise. With an estimated 10 billion people on the earth by 2050, energy consumption is only expected to increase. Fossil fuels, which include oil, coal and gas have been widely used to generate energy and are predicted to do so at least until 2050 (see Fig.6.1) [1]. Figure 1. Global primary energy consumption is broken down by energy source [2, 3]. These fuels generate a large amount of greenhouse gases (GHGs) during combustion, especially carbon dioxide (CO2), which is connected to climate change and global warming. In addition, burning fossil fuels releases particulate matter (PM), sulfur oxides (SOx), nitrogen oxides (NOx), and other pollutants that are harmful for human health. In addition, the unequal distribution of fossil fuel supply has led to increased instability globally and worries about energy security. Numerous nations started making investments in energy from renewable sources as concerns about the environment and energy security have grown. Because they emit little to no greenhouse emissions, renewable energy resources like hydroelectricity, wind, and solar power contribute to lowering the energy industry's overall environmental impact (see Fig.6.2) [4].

Introduction 
Nanotechnology has become a highly influential field in science, showcasing diverse applications and promising trends over the past few years. Nanoscience has been a source of inspiration for material science researchers, encouraging the exploration of innovative methods to synthesise novel structures with significant properties and applications(Kolahalam et al. 2019). Nanoparticles are the core of nanotechnology, with “nano” denoting sizes smaller than a billionth of a hair, specifically below 100 nm (Volath et al. 2008).The focus on small-sized nanoparticles (1–100 nm) has become a global research priority attributable to their remarkable applications in physical, chemical, and biological sciences (Jeevanandam et al., 2018). The term “nanotechnology” was coined by Nobel Prize-winning physicist Richard Feynman in his renowned 1959 lecture titled “There’s Plenty of Room at the Bottom.”(Boverhof et al., 2015).Feynman proposed the advanceof smaller devices down to the molecular scale, asserting that it was not the laws of nature but rather the lack of appropriate equipment and approaches that limited progress in working at atomic and molecular scales(Clunan et al., 2014). In recent times, nanotechnology has transitioned into a mainstream and crucial innovation. When materials are manipulated at the nanoscale, their properties undergo significant transformations, leading to remarkable applications in physics, space science, chemistry, medicine, energy renewal, healthcare, environmental science, mechanics, electronics, civil engineering, optics, and more [Figure. 1](Mourdikoudis et al., 2018; Joshi et al., 2008; Suresh Kumar et al., 2019); AarthyePandian et al., 2019).

Group dynamics and formation represent fundamental processes in community mobilization that determine how individuals come together to form collective entities capable of achieving shared goals while building social capital and institutional capacity that can sustain long-term community development efforts. Understanding these processes provides essential insights into how communities can organize themselves effectively while creating groups that can navigate complex challenges and maintain cohesion through the inevitable difficulties and conflicts that arise during collective action initiatives. The science of group dynamics encompasses the complex social, psychological, and organizational factors that influence how groups form, develop, function, and achieve their objectives while building understanding of the internal processes that enable or constrain group effectiveness and sustainability. This knowledge has evolved through decades of research in social psychology, organizational behavior, and community

Place of Microorganisms in Living World

Haeckel’s kingdom Protista

E.H. Haeckel, 1866 proposed this concept. Apart from plant and animal kingdom, the third kingdom “Protista” was developed by him. The protista includes unicellular organisms that are typically neither plant nor animal. These organisms, the protists, include bacteria, algae, fungi and protozoa. Bacteria are referred as lower protists and others are called as higher protists.

Whittaker’s five kingdom concept

R.H.Whittaker (1969) proposed the concept of five kingdom. He divided the living things in to 5 kingdoms.

Regulations should make it easier to utilise creative, long-lasting solutions, allowing for the selection of the most environmentally friendly pest management method. This can be accomplished by using expedited registration, priority registration, and a combination of comparative evaluation of pest control techniques and the substitution principle, which allows a natural pest control technique to take the place of a synthetic pesticide.

Introduction

The rumen ecosystem is essentially a fermentation system that houses a vast array of different microbes. Ruminants typically browse low energy forages, such as grasses or other plant material, rich in fibre but not easily accessible to the ruminant without rumen fermentation. These forages are literally food for the microbes present in the rumen first and then, in turn, the products of these microbes are the feed for the ruminant. Microbes break down forage in the anaerobic rumen environment and the products of microbial fermentation provide the ruminant with metabolisable compounds, such as short chain fatty acids, while the microbes themselves account for around 90% of the amino acids entering the lower intestine. There are many significant challenges to investigate rumen ecology.

Introduction 
Due to the depletion of their raw material supplies, traditional fossil fuel use should be reduced. There is significant hope for biofuel production (Gangl D et al., 2015 ; Seo et al., 2017), and in the past decade, interest in using microalgae has been steadily increasing (Gangl D et al., 2015). The algae group consists of various photosynthetic eukaryotic species that are typically found in both freshwater and marine environments. With an estimated 30,000 to 1 million species, algae range from single-celled cyanobacteria to multicellular forms, each having distinct traits. Microalgae, specifically, grow rapidly and absorb carbon dioxide as they develop (Guiry and M. D., 2012). Many industries have benefited from the utilization of microalgae species for the good of humanity. Food, medications and antibiotics, wastewater treatment, biofuel, biofertilizers, and CO2 fixation are a few of the significant uses for microalgae (Singh et al., 2018 ; Ananthi et al., 2021). Compared to first-generation biofuels, the manufacturing of algae-based biofuel is more advantageous to the environment (Alalwan et al., 2019). Microalgal biofuel production is a greener alternative to first-generation biofuels (Alalwan et al., 2019). It can be cultivated in less ideal locations, such as the ocean or non-arable land, and doesn’t require fertile soil, which helps prevent rivalry with food crops for freshwater and farmland (Winckelmann et al., 2015; Correa et al., 2020). Microalgal production systems can help treat wastewater or convert CO2 into oxygen without interfering with the food supply (Onyeaka et al., 2021; Prasad et al., 2021). Nanotechnology and nanomaterials are becoming increasingly popular across many fields for their useful properties, with global research exploring their role in biodiesel production and processing. To speed up the development of biodiesel production, advanced nanotechnology is being emphasized to achieve higher yields at lower costs. Microalgae are a promising source for biodiesel production, and various nanoparticles can significantly boost the efficiency of harvesting them. Reusing nanomaterials and combining cell harvesting, disruption, and extraction processes also help reduce costs. Moreover, different nanocatalysts can enhance the efficiency of biodiesel conversion (Verma and Kuila, 2020). Currently available techniques for microalgal harvesting include chemical, mechanical, biological, and electrical ones to some extent. Harvesting microalgal biomass is most often done by mechanical means since they are the most dependable (Grima et al., 2003 ; Christenson et al., 2011). But, in order to increase productivity and reduce operating and maintenance expenses, these procedures are frequently followed by biological or chemical flocculation phases. For maximum separation efficiency and minimum cost, two or more of these techniques are frequently used. For instance, processing costs can be significantly decreased by combining centrifugation and flocculation-sedimentation (Schlesinger et al., 2012). Novel approaches like biological treatments have the potential to further lower operating expenses.

Herbal Based Formulations presents plant-derived preparations used for pest, disease, viral-vector, growth, and development management. The chapter includes neem seed kernel extract, Vitex, onion, tobacco, chilli-garlic, ginger, custard apple, eucalyptus, lantana, mint, marigold, papaya, aloe, pomegranate waste, and other botanical preparations. It organizes formulations according to their intended agricultural function and provides examples of decoctions, extracts, sprays, and fermented products. The chapter demonstrates the diversity of locally available plant resources that can be incorporated into natural farming practices. It also highlights the practical importance of botanical inputs for reducing dependence on synthetic crop-protection products while supporting sustainable farm management. effectively.

The biocontrol use had almost completely disappeared due to the growth and success of the synthetic pesticide industry in the mid-1940s. The publication of Rachael Carson's 'Silent Spring' (Carson 1962) which condemned the use of agricultural pesticides and emphasized their harmful environmental effects on wildlife. Due to public outrage over this controversial book, there was a need for pesticide alternatives, which presents an opportunity for wider use of biological control (Barratt et al. 2010; Gay 2012).

History of Soil Microbiology

There is enough evidence in the literature to believe that microorganisms were the earliest of the living things that existed on this planet. Man depends on crop plants for his existence and crop plants in turn depend on soil and soil microorganisms for their nutrition. Scientists from the beginning studied the microorganisms from water, air, soil, etc. and recognized the role of microorganisms in natural processes and realized the importance of soil microorganisms in the growth and development of plants.Thus, microorganisms have been playing a significant role long before they were discovered by man. Today, the soil is considered to be the main source of scavenging the organic wastes through microbial action and is also a rich storehouse for industrial microflora of great economic importance.

Unlike soil science whose origin can be traced back to Roman and Aryan times, soil microbiology emerges as a distinct branch of soil science during the first half of the 19^th century. Some of the notable contributions made by several scientists in the field of soil microbiology are highlighted in the following paragraphs.

Anton Von Leeuwenhoek (1673) discovered and described microorganisms through his own made first simple microscope with a magnification of 200 to 300 times. He observed minute, moving objects which he called “animalcules” (small animals) which are now known as protozoa, fungi, and bacteria. He for the first time made the authentic drawings of microorganisms (protozoa, bacteria, fungi).

1.1 Introduction
The concept of invention is the fundamental requirement for design thinking. Since the birth of mankind, people started inventing many concepts, which are mainly observations of facts and innovation of ideas for producing products that are useful to the society. The design thinking requires unconventional thinking to design processes for maximal operational efficiency of manufacturing the products, which give maximum satisfaction to customers with more value for money. Hence, the team that is involved in the design thinking projects may gain the required skills by knowing several inventions of the past. Hence, a review of inventions is presented in this chapter.
1.2 Tracing Inventions
The content at https://www.explainthatstuff.com/timeline.html gives a complete account of inventions starting from 4–5 billion years ago up to 2022. The article on solar cell energy gives an account of the sun started producing energy. About 1.2 million years ago, humans invented fire, which has been further innovated for biofuels, candles, IC engines, aviation engines, etc. In the 7th century B.C., ants were killed by burning in the heat produced by concentrated sun rays using magnifying lens. Greeks and Romans utilised burning mirrors to light candles during religious ceremonies in the third century B.C. About 3.5 million years ago, stone, wood, antlers, and bones were used by humans to make first kind of tools. These were further improved to make tools for machines. About 4000 BCE back, iron was used to make decorative ornaments. About 3500 BCE, people invented the concept of wheel, which brought quantum revolution in transportation.

Obviously human have had to deal with microbes even before the recorded history. The first record of human using microorganisms comes from ancient tablets from mid east.

Babylonians were using yeast to make beer over 8000 years ago and acetic acid bacteria to make vinegar over 6000 years ago. About 5000 years ago, Persia (Now Iran) region recorded the wine making.

The Romans had God for specific microorganisms. The roman God of mold and mildew were “Robigus” and “Robigo” which mean crop rust. (Rust is one of the plant disease caused by fungus). God Robigus was very much feared because of crop lost. About 2000 years ago, Romans proposed that diseases were caused by tiny animals. But, fundamentalist religions had a strong hold over the progress. The real microbiology history starts from 1600s, when people began to make crude lenses and microscopes.

The following are the scientists and their role in the development of microbiology: