eChapters

1. Introduction:- Ensuring Quality and Success in Assisted Reproductive
Technology through Good Laboratory Practices Assisted Reproductive Technology (ART) in animals has become an increasingly vital set of techniques with wide-ranging applications across agriculture, wildlife conservation, and biomedical research. These technologies, encompassing procedures such as in-vitro fertilization (IVF), embryo transfer, and cryopreservation of gametes and embryos, offer solutions to infertility, enable genetic improvement in livestock, and play a crucial role in preserving endangered species. As the application of ART expands throughout the globe, the need for stringent quality control and standardization becomes paramount to ensure the reliability and reproducibility of outcomes. Good Laboratory Practices (GLP) represent a well-established quality system that provides a framework for the organizational processes and conditions under which non-clinical studies are planned, performed, monitored, recorded, reported, and archived. Originally developed to ensure the integrity of safety data for pharmaceuticals and other regulated products, the fundamental principles of GLP are highly relevant to the field of animal ART. This report aims to explore the application of GLP in ART and to thoroughly investigate its contribution to achieving successful outcomes in various ART procedures and across different animal species. By examining the core principles of GLP and their practical implementation in animal ART laboratories, this analysis will highlight the critical role of standardized practices in enhancing the reliability, ethical conduct, and overall success of animal reproductive technologies.
2. Good Laboratory Practices (GLP) in Reference to ART
Good Laboratory Practices are broadly defined as a quality system that governs the organization and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, reported, and archived. This system is designed to ensure the quality and

Introduction
Defining GLP: Good Laboratory Practices (GLP) are a comprehensive set of quality system principles designed to ensure the reliability, reproducibility, and integrity of non-clinical health and environmental safety studies. These principles establish a framework for planning, performing, monitoring, recording, reporting, and archiving studies. Adherence to GLP is crucial for generating data that can be trusted and utilized for regulatory submissions and scientific advancements.
Purpose of GLP
• To ensure the quality and integrity of non-clinical and clinical laboratory studies.
• To provide assurance that data submitted to regulatory authorities is truly reliable.
• To standardize procedures and practices in non-clinical and clinical studies.
Scope of GLP
• Primarily applies to non-clinical health and environmental safety studies.
• These studies often support applications for research or marketing permits for regulated products (e.g., pharmaceuticals, pesticides, food additives).
Key Elements of GLP
• Organizational structure and personnel responsibilities.
• Standard operating procedures (SOPs).

Introduction 
Toxicological studies play a crucial role in assessing the safety and potential hazards of chemicals, pharmaceuticals, and environmental pollutants. To ensure the reliability, consistency, and integrity of such studies, the concept of GLP has been established. GLP is a set of principles that govern the planning, execution, monitoring, recording, and reporting of laboratory experiments, particularly those related to safety assessments. The primary objective of GLP in toxicological studies is to ensure that the data generated is of high quality, reproducible, and acceptable to regulatory agencies worldwide. This is essential for evaluating the potential risks associated with exposure to various substances, aiding in the development of safer drugs, chemicals, and consumer products. Compliance with GLP guidelines minimizes errors, prevents fraudulent practices, and enhances transparency in research, ultimately contributing to public health and environmental safety. The assessment of potential hazards posed by chemical, pharmaceutical, agrochemical, and other substances to human health and the environment lies at the heart of toxicological studies. These investigations, conducted in non-clinical settings, provide crucial data that informs regulatory decisions, guides product development, and ultimately safeguards public well-being. The consequences of inaccurate or unreliable toxicological data can be far-reaching, potentially leading to the approval of unsafe products or the unwarranted restriction of beneficial ones. Recognizing the critical importance of the integrity of these studies, the concept of Good Laboratory Practices (GLP) has emerged as a cornerstone, establishing a robust framework for their planning, conduct, monitoring, recording, reporting, and archiving

1. Introduction: Setting the Stage for GLP in Artificial Insemination
Artificial Insemination (AI) stands as a cornerstone technology in modern animal agriculture, playing a pivotal role in the genetic enhancement of livestock across a diverse range of species. This technique, particularly prevalent in cattle and swine, allows for the widespread dissemination of superior genetic traits from carefully selected sires, leading to significant improvements in productivity, such as increased milk yield in dairy cattle and enhanced growth rates in beef cattle. Beyond genetic advancement, AI offers considerable advantages in disease management by mitigating the risks associated with natural mating, thereby controlling the transmission of reproductive tract infections. Furthermore, AI provides economic benefits to livestock producers by reducing the need to maintain live bulls, which require substantial resources, and by offering access to a broader spectrum of genetics than would be feasible through natural service alone. The application of AI is not limited to traditional livestock; it is increasingly employed in the breeding of horses and smaller ruminants, as well as in the management of pet populations, indicating its growing importance in animal reproduction across various sectors. The expanding scope of AI underscores the necessity for robust quality control measures to ensure its efficacy and safety across different animal species and production systems. The success of any AI program is intrinsically linked to the quality of the semen used, making the operational standards of AI laboratories paramount. Optimal conception rates rely on semen with high viability, motility, and morphological integrity, which can be compromised by inadequate handling and unsanitary 130 Concepts of Good Laboratory Practices (GLP) conditions during collection, processing, storage, and insemination. Poor semen management can lead to reduced fertility and the potential spread of diseases, thereby undermining the benefits of AI. To ensure consistent and successful outcomes, standardized procedures throughout the AI process are essential, providing a framework for optimizing techniques and minimizing the risk of errors. In this context, Good Laboratory Practices (GLP) emerges as a critical quality paradigm. Defined as a managerial quality control system, GLP encompasses the organizational processes and the conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, archived, and reported. While traditionally applied to the safety testing of chemicals and pharmaceuticals, the core principles of GLP, with their emphasis on systematic processes, controlled conditions, and rigorous data management, hold significant relevance for ensuring the quality and reliability of operations within animal AI laboratories. The genesis of GLP regulations in response to concerns about the integrity and consistency of scientific data further underscores its fundamental importance in any laboratory setting where the trustworthiness of results is paramount. This report aims to provide a comprehensive analysis of the GLP paradigm within the context of animal AI laboratories. Its objectives include defining GLP and its core principles, investigating the specific application of these principles in the various stages of animal AI, exploring the benefits and challenges associated with GLP implementation, reviewing relevant regulatory guidelines and standards across different regions, analyzing the impact of GLP adherence on AI techniques and outcomes, identifying effective data representation strategies, comparing the operational differences between GLP and non-GLP compliant laboratories, and discussing emerging trends and future perspectives on the role of GLP in advancing the field.

Introduction

India is transforming from the phase of revolution to commercial mode of agriculture, a step ahead of self-sufficiency. It further evolves to reach a goal of self-reliant on agriculture and allied sector. The present phase demands several technological innovations, their application and implementation in the market. Meanwhile this phase needs due attention for handling the issues on conservation of natural resources, especially germplasm resource, biodiversity conservation, socio-cultural-economic values of our country, etc. The whole process requires good investment on research and development, various economic measures and implementation. It is only possible with vibrant governance and policy implementation of a Government. Already Government of India has taken several measures to transform agriculture. Recent analysis showed that Indian agriculture is the silent ever green revolution. Food production is kept pace with the growing population since independence. Further, it needs several innovations on technologies coupled with policy support to fill the demand. India is placed 49^th position in the world on innovations. Analysis suggested the huge potential for innovation due to its huge human capital, which perhaps need for the country like India to meet the food demand of 333 million tonnes by the year 2050. These innovations must be focussed for growth to efficiency, employment generation, food security to nutrition and health, shortage management to surplus management, input intensive to knowledge intensive agriculture, climate change and sustainability, production and producers, policy interventions, regulations and reforms. The Government emphasize usage of e-trading platforms to sell the farmers’ produce of their choice rather using mandis. This will enable contract farming and inter-state trade. However, major innovations and investment must be implemented on transportation, logistics, and digital access and payments to enable widespread prosperity. Thus, focus must be on the complete agricultural value chain. In fact, focus needed on value addition, rural infrastructure, logistics, warehousing, and quality certification of agriculture produce. India has greatest challenges to face in the future for food and nutritional security, adaptation and mitigation to climate change and sustainable use of essential and scared resources like water, land and energy.

The rapid adoption of Large Language Models requires organizations to manage not only model performance but also ethical, regulatory, operational and lifecycle risks. This chapter presents an integrated perspective on governance, optimization and lifecycle management of LLMs. It examines governance principles based on fairness, accountability, transparency, human-centered design and responsible AI. Regulatory developments and policy gaps are considered alongside the challenges of auditing opaque models, controlling hallucinations and establishing accountability across organizations and jurisdictions. The chapter also explores optimization methods such as pruning, quantization, model compression, instruction tuning, LoRA and QLoRA to improve efficiency while maintaining useful capabilities. Lifecycle management extends from data collection and model design through training, evaluation, deployment, monitoring, updating and eventual decommissioning. Real-world cases demonstrate the importance of continuous testing, feedback, security controls and governance throughout the model lifecycle. Emerging threats, including prompt injection, data poisoning, misinformation, privacy leakage and malicious use, further emphasize the need for coordinated oversight. The chapter ultimately advocates a lifecycle-based governance framework combining technical optimization, regulatory compliance, ethical safeguards and continuous monitoring

The Tenth Five Year Plan (GoI 2002), for 2002 through 2007, has put emphasis on natural resource management through rainwater harvesting, groundwater recharging measures and controlling groundwater exploitation, watershed development, treatment of waterlogged areas. With regard to application of agricultural inputs like fertilizer and pesticides, the Plan stated that factors such as imbalanced use of nitrogenous (N), phosphatic (P) and postassic (K) fertilisers, increased deficiency of micronutrients and decreased soil organic carbon would be addressed through a holistic agri-environmental approach stressing Integrated Plant Nutrient and Pest Management. Further, the Tenth Plan document recognizes organic farming as a ‘thrust area’ in the sustainable use and management of resources in agriculture.

The trajectory of Indian agriculture and its associated environmental problems has brought about recognition that future agricultural growth and productivity will have to occur simultaneously with environmental sustainability. The environmental challenges, especially in terms of land degradation and groundwater depletion, water logging and excessive use of chemical inputs are posing problems for the future of Indian agriculture. To address the problems, policies have laid emphasis on promoting sustainable agriculture including organic farming. Differential approaches and policy instruments, however, will be required to address these problems. The shift from input-intensive to sustainable, particularly organic farming is a difficult task as it involves a number of policy measures dealing with a variety of issues ranging from the transfer of information and technology to the development of markets. Another difficult task, and perhaps more difficult, relates to marginal and small farmers – which comprise a substantial part of Indian agriculture. Although these marginal and small farmers have been considered organic by ‘default’, severe resource constraints make a shift to the modern sense of organic farming prohibitive.

The Indian government has been undertaking measures to promote organic farming with the aim to improve soil fertility and help to double the farmers’ incomes by the year 2022. The Prime Minister had visited Sikkim—which is India’s first organic state—and encouraged other states to replicate the “Sikkim model”. Some of the policy initiatives to promote organic farming and exports include development of an organic regulation for exports by the Agricultural and Processed Food Products Export Development Authority (APEDA), removal of quantitative restriction on organic food exports, providing subsidies to farmers under the Paramparagat Krishi Vikas Yojana (PKVY) in partnership with the state governments, and other schemes such as the Mission Organic Value Chain Development for North Eastern Region. Despite these initiatives, a recent survey-based study covering 418 organic farmers across different states of India suggests that a move to organic farming methods may not be that easy and organic farmers are not getting the expected premium price for their produce.

Introduction

Sustainable agriculture focuses on practices that ensure the long-term well being of the environment, society, and economy. In India, the government has instituted various policies and initiatives to encourage sustainable farming practices and enhance farmers' income. Embracing sustainability is crucial for making agriculture both profitable and environmentally friendly for future generations, and technology emerges as the key enabler for achieving this goal. Technological advancements empower farmers to make informed decisions regarding optimal planting and harvesting times, as well as the judicious use of water and fertilisers. This leads to reduced waste, increased crop yields, and ultimately higher profits for farmers. Additionally, technology plays a significant role in mechanisation, encompassing the use of modern agricultural implements that multitask while minimising soil impact (McFadden et al., 2023). Addressing major challenges such as crop residue management through environmentally friendly technological methods not only enhances efficiency and productivity but also reduces the physical labor required, transforming farming into a less strenuous occupation.

The successful transformation of agriculture through artificial intelligence and digital technologies requires supportive policies, institutions, infrastructure, and farmer-oriented programmes. This chapter examines the role of government policies and institutional support in promoting smart farming and digital agricultural innovation. Effective policies can facilitate investment in research and development, digital infrastructure, farmer training, technology access, rural connectivity, data governance, and sustainable agricultural practices. Institutional mechanisms are particularly important for overcoming barriers faced by smallholder farmers, including high technology costs, limited digital literacy, and inadequate infrastructure. The chapter emphasizes collaboration among governments, research institutions, universities, private enterprises, and farming communities to promote inclusive technology adoption, strengthen resilience, and achieve sustainable food security.

India is a leading producer and exporter of onion, but its productivity, market stability and post-harvest efficiency remain constrained. Farmers face fragmented holdings, climate uncertainty, price fluctuations, inadequate storage, technological gaps and significant post-harvest losses. This chapter reviews government programmes and policies related to financial support, irrigation, infrastructure, crop insurance, market access, farmer organizations, storage, processing and agricultural research. It also discusses export-quality requirements, grading, packaging and pesticide-residue standards. Research priorities include stress-resistant varieties and hybrids, drip irrigation, fertigation, growth regulation, sprout control and non-thermal storage treatments. Coordinated policies and technological dissemination can improve farmer income, productivity, quality and market resilience.

Underutilized crops can be found in many different agricultural ecosystems, but they are mainly grown by small landholders in the marginal areas especially in hill agriculture. Some crops remain underutilized because they are in some way not competitive with other species in the same agricultural environment. They are usually characterized by having local importance in consumption and production systems, requiring relatively low inputs, adapting to specific agro-ecological niches, receiving scarce attention by national agricultural and biodiversity conservation efforts, mainly consisting of local types or landraces, and being cultivated with indigenous knowledge. Many underutilized species are adapted to low-input agriculture and depended on by a large number of people in marginal areas in hill agriculture of developing countries.

This chapter attempts to provide a detail picture of one such underutilized crop i.e. Grain Amaranth. In an effort to explain the decrease in grain amaranth cultivation, fanciful myths have arisen. The mystery is especially intriguing when one considers that maize, with which amaranth co-evolved, was selected and developed into a major world crop. The small seed size of amaranth may have been a partial cause for the reduction in amaranth cultivation. A small seeded crop, such as amaranth, requires greater attention to detail in the early parts of the growing season than does a larger seeded crop, such as maize.

This chapter discussed all the factors related to its cultivation, the constraints in the production and importance of grain amaranth in the economy of hill agriculture etc.

Abstract
Grain legumes, also known as pulses, are vital to sustainable agriculture due to their roles in enhancing soil fertility, providing essential nutrition, and supporting environmental sustainability. By establishing symbiotic relationships with nitrogen fixing bacteria, legumes naturally enrich soil with nitrogen, reducing reliance on synthetic fertilizers and fostering sustainable farming practices. Their inclusion in crop rotations not only boosts soil health but also supports food security, especially in developing countries where they are a key source of plant based protein and nutrients. The impact of climate change, including rising temperatures, altered precipitation patterns, and increased frequency of extreme weather events, presents significant challenges to agriculture. In this context, grain legumes demonstrate considerable climate resilience. Their drought tolerance, heat resistance, and ability to fix atmospheric nitrogen make them well-suited to withstand climate stress. Legumes exhibit mechanisms such as deep root systems, efficient water use, and adaptability to diverse environmental conditions, contributing to their robustness in the face of climate variability. To bolster the role of grain legumes in climate-resilient agriculture, climate-smart practices and technological advancements are essential. These include optimizing planting strategies, adopting water-efficient methods, and breeding for improved resilience traits. Addressing challenges such as climate variability, pest pressures, and resource limitations is crucial for maintaining the benefits of legumes. Future efforts should focus on developing robust management
practices and improved varieties to ensure the continued contribution of grain legumes to food security and environmental sustainability.
Keywords: Climate Resilience, Drought Tolerance, Grain Legumes, Heat Resistance, Sustainable Agriculture, Water Use Efficiency

Abstract
Grain legumes, a group of crops which belong to Fabaceae family, are primarily grown for their edible seeds. Pulses contain a high amount of protein (20-25%), vitamins, and minerals. Consumption of pulses can be beneficial for cardiovascular health, weight management, for diabetics, cancer management, bone health etc. So, grain legumes can play a significant role to address future global food security, environmental sustainability, and nutritional needs. The nutritional value of grain legumes can be significantly enhanced through biofortification, agronomic intervention, processing, and value addition. So, diversification of food habit with pulses and cereals can solve the prolong problem of malnutrition in developing countries including India. Despite such great importance, global food legume production has
been found to increase at a very slow pace. Keywords: Biofortification, Environmental sustainability, Food security, Grain legumes, Nutritional security
Introduction
Grain legumes or pulses are a group of crops which belong to family Fabaceae, are primarily grown for their edible seeds. The common examples of grain legumes include: bean (Phaseolus spp.), lentil (Lens culinaris), chickpea (Cicer arietinum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupinus spp.), broad bean (Vicia faba), igeon pea (Cajanus cajan), grass pea (Lathyrus sativus), black gram (Vigna mungo), green gram (Vigna radiata). Grain legumes can play a significant role to address future global food security, environmental sustainability, and nutritional needs. Pulses are the second most important food crop after cereals and an important source of proteins, vitamins, and minerals. Pulses are popularly known as “Poor man’s meat” and “Rich man’s vegetable”. Pulses contain a high amount of protein (20-25%), which is almost double and triple of the protein content of wheat and rice, respectively and so can contribute significantly to the nutritional security of the country. Besides, they may serve the purpose of protein rich fodder and good quality green manure crop. Pulses can thrive well in low and marginal land with less external inputs. They act as erosion resisting crops because of its deep tap root
system, leafy growth, and close spacing. Pulses can also be grown as paira crop or inter-crop in different growing situations. Being a leguminous crop, it can fix atmospheric nitrogen with the help of its root nodules, resulting in low requirement of external fertilizer. Keeping the importance of pulses in mind, United Nation’s Food and Agricultural Organization (FAO) also declared 2016 as the ‘International Year of Pulses’.

Cereals and pulses are the staple food in India, thus it has to be stored by producers in their homes and by traders and government agencies. Variations in the season i.e., warm and high humidity weather is highly conducive to proper development and rapid multiplication of storage insect pests. Almost all insect pests of stored grains have a remarkably high rate of multiplication and within one season, they may destroy 9-10% of the grains and contaminate the rest with undesirable odors and flavors. The type of insects infesting grains vary and include weevils, moths and beetles. Apart from this it is necessary to control or kill egg, larval, pupal and adult stages of the insect. After proper cleaning and drying the produce is stored where damage can be caused due to cross-infestation or infestation already left in the produce. Grains are stored in different types of receptacles and to protect it, different protectants are being used since from the early days. The success in the use of these protectants (organic, inorganic or inert) depends upon the effect on the grain, killing of the insects, practicability, period of storage and lastly the physical and chemical state of treated seeds.

1. Inert dusts

Inert dusts are fine powders chemically inactive but cause considerable insecticidal activity by mechanical abrassing and physical absorption of moisture proofing layers of insect integument. The use of inert dust was initiated sometimes during World War II. Some common inert dusts are ashes, clays, gypsum, lime, magnesite, Nakki, silica, talk, phosphates, diatomaceous earth. It was reported that though most of the dust proved effective and convenient to use but it is difficult to remove it and clean as it goes inside the infested and damaged grains. The dust can only kill larvae and adults moving in between the grains. Once any stage of insects entered inside the grain, it is beyond the reach of the dust.

Solution:

Steps used for plotting the bar graph are as:

Step 1: Open Excel sheet and feed the above data as given in figure. 9.1.

Step 2: Select data and go to insert menu and select column chart. (figure 9.2)

Step 3: Select 2-D column, option first from dialog Box (figure 9.3).

Step 4: The desired output will be as shown (figure 9.4).

Remark The data labels, axis name, title of the graph etc. can be inserted through selecting option formatting chart area and add data labels by right clicking on the graph output (figure 9.5).

The growing demand for sustainable materials has encouraged the development of green composites based on bio-based polymers and renewable reinforcements. This chapter explores advanced carbon nanomaterials, including graphene, graphene oxide, carbon nanotubes, carbon nanofibers, and carbon quantum dots, as functional reinforcements for green composites. It discusses their synthesis, functionalization, incorporation into biopolymer and bio-based matrices, and influence on mechanical, electrical, thermal, and barrier properties. Particular attention is given to green synthesis approaches using biomass-derived resources, surface modification, dispersion, environmental considerations, scalability, cost, and life-cycle assessment. The chapter also considers future opportunities for multifunctional and commercially viable sustainable nanocomposites.

Graphene and related carbon nanomaterials have attracted considerable attention as reinforcements for high-performance green composites. This chapter examines graphene, graphene oxide, reduced graphene oxide, carbon nanotubes, and graphene nanoplatelets and their integration with bio-based polymers and natural fibres. It discusses synthesis and fabrication methods, characterization techniques, mechanical properties, electrical and thermal performance, barrier properties, and applications. The chapter also considers dispersion and compatibility challenges associated with incorporating nanomaterials into green matrices. Graphene-based composites are explored for energy storage, environmental treatment, biomedical applications, sensors, packaging, automotive, and aerospace uses. Emerging trends focus on multifunctionality, scalable production, cost reduction, and sustainable processing.

Green business, is enterprise that has no negative impact on the global or local environment, community, society, or economy—a business that strives to meet the triple bottom line. Often, sustainable businesses have progressive environmental and human rights policies. In general, business is described as green if it matches the following four criteria:

1. It incorporates principles of sustainability into each of its business decisions.

2. It supplies environmentally friendly products or services that replace demand for non-green products and/or services.

3. It is greener than traditional competition.

4. It has made an enduring commitment to environmental principles in its business operations.

A sustainable business is any organization that participates in environmentally friendly or green activities to ensure that all processes, products, and manufacturing activities adequately address current environmental concerns while maintaining a profit. In other words, it is a business that “meets the needs of the present world without compromising the ability of the future generations to meet their own needs.” It is the process of assessing how to design products that will take advantage of the current environmental situation and how well a company’s products perform with renewable resources.

Green composites are emerging as promising alternatives to conventional synthetic composites in response to climate change, resource scarcity, environmental degradation, and increasing demand for sustainable materials. This chapter reviews the current landscape of green composites and examines the challenges associated with their broader adoption. It discusses technological innovations, smart sustainable materials, bio-based nanocomposites, hybrid systems, environmental and economic implications, and the relationship between green composites and sustainable development. Processing limitations, high costs, regulatory uncertainties, and commercialization challenges are considered alongside opportunities for technological advancement. Life-cycle assessment and supportive policies are important for evaluating environmental impacts and accelerating the transition toward circular and sustainable material systems.