eChapters

Abstract

The advent of modern chemical industry has resulted in the release of huge amounts of novel organic compounds, as industrial by-products, pesticides, other agrochemicals etc into the environment. These new compounds tend to resist biodegradation, with potential consequences such as persistence in the environment or bioaccumulation in food chains. Early detection of toxic chemical compounds in the environment, particularly in water, and their biological effects on organisms has therefore become increasingly important. The traditional approach to environmental pollution assessment is based on chemical analytical methods which only provide information about the absolute concentrations of known chemicals in the environmental sample without an adequate interpretation of its toxicity to biota. In order to get more relevant information about environmental pollution risk, it is therefore inevitable to supplement the chemical analytical data with the results of methods providing information on biological impacts. The negative biological effects of pollutants present in all kinds of environmental samples can be assessed using different living organisms or cells as ‘analytical devices’. The biological response following the exposure of living organisms or cells to environmental sample usually gives an information on toxicity, genotoxicity, estrogenicity etc. of the whole mixture of chemical compounds. According to the technical principle, methods of biological monitoring can be classified to bioassays, biosensors, immunoassays, and ecological methods. Besides being sensitive only to the bioavailable fraction of pollutants, biotests also have the power to assess the integrated effect of interacting chemical compounds and to detect the compounds, which are toxic only due to bioactivation.

9.1. Molecular breeding and marker assisted selection

Using conventional breeding, developing new varieties takes atleast 15-20 years.

Molecular breeding shortened the time to 7-10 years.

One of the tools for faster variety development is Marker Assisted Selection (MAS).

MAS-refers to use of DNA markers to assist phenotypic screening.

Plants that possess particular genes or quantitative trait loci (QTL) may be identified based on their genotype rather than their phenotype.

During most of the years, there will be loss of agricultural production by at least 15 to 20 per cent per annum due to unfavourable weather situations. The farmers practicing agriculture expect uniform returns every year as the investment costs are much higher and cannot afford loss in production. Of late, the inter-seasonal and intra seasonal variations in weather are very frequent. The weather conditions observed were found to exceed even beyond known limits. Exceptional deviation from any one single weather parameter like air temperature, rainfall and wind can trigger considerable loss in production or sometimes even crop failure (Fig.12.1). Fig. 12.1: Components of a microclimate and its interlinkages Large scale weather modification is not practicable to a greater extent as uniform results cannot be expected under these conditions. There is need to explore cost effective management options that may significantly alter some weather parameter that is detrimental to crop production at field level by the farmers themselves. Therefore, microclimatic modification is an emerging technology to alter or change the unfavourable weather conditions detrimental to crop growth using cost effective and easily manageable techniques for minimizing the loss in crop production. The options for microclimatic modification have to be decided based on whether and the nature of weather aberrations. For example, dry spells are most common during rainy season in most of the arid and semi-arid regions. The farmers growing crops under rainfed conditions suffer considerable loss in production due to occurrence of dry spells as the crop experiences moisture stress. These dry spells may occur any time during the growing season. In such situations, the farmer needs to adopt a management option even before sowing a crop. Sometimes, the occurrence of weather aberration cannot be anticipated and the farmer has to prepare for adopting a management option based on weather forecast. Such measures are temporary and needs to be adopted to ward off the unfavourable weather conditions to a certain extent. Microclimatic modification can be a permanent, seasonal or temporary option depending upon the climate of the region. The crop microclimate refers to the climate just above and within the crop canopy and in the soil root zone that can be influenced by the day-today management practices. Microclimate modification is a measure to change or regulate the weather parameter at farm level. The microclimate management option also depends upon the nature and type of the soil as well.

Abstract

Tremendous hike inworld population has increased thrust on the limited resources which has considerably affected the viability of the food. The emerging challenge for policy makers and food scientists is to put forward viable solutions. Utilization of underutilized crops/plants that act as good sources of starch could be explored in a better way for the sustenance of the limited sources like food crops/plants. The demand for starch and the foods containing starch has been increasing continually. Since, the starch in its native form has certain limitations in its functional properties to be used for different applications. Therefore, it becomes important to explore certain technologies which could be used for modification of starch. Among different technologies gamma irradiation ensures food safety while inducing physicochemical changes of the components. Although, the chemical modifications have been used widely so far but the concerns related to the chemical residues left are diminishing their demand. Hence, the physical processes particularly the gamma irradiation process has emerged as an efficient alternative with some benefits over other process like eco-friendly, economical, reliable and safe for altering the physico-chemical and biological characteristics of the product. Gamma Irradiation of starch has been successfully employed for the achievement of some desired physico-chemical, biological and functional properties of the foods and other related items. In this chapter, valuable impact of gamma irradiation on the physico-chemical, biological and functional properties of starch has been summarized.

8.1. Documented ITKs on Moisture Conservation

The ITKs were documented under following specific categories

• Agronomic Measures
• Tillage
• Bunding & Terracing (Mech. & Vegetative barrier)
• Land Configuration
• Soil Amendment / Mulching
• Erosion Control & Runoff Diversion Structures
• Water Harvesting, Seepage Control & Ground Water Recharge

India is an agricultural country. The geographical area of India is about 328 mha out of which 147 mha of land under cultivation and 84-mha land is under irrigation. The population is increasing at an alarming rate that requires producing more food for the growing population. This problem can be solved either by increasing the land under irrigation or using different soil water conservation techniques. It is well known fact that during rainfall a considerable amount of water goes to sea due to high rate of runoff. It is highly needed to trap as much as water falling in the form of rainfall and recharge the groundwater so that it is made available for irrigation purposes.

Moisture plays by far the most important role in soils as regards vegetation. It is through the medium of water that the plants obtain their quota of mineral food vital for their growth. If water is gradually withheld from the soil, at a certain stage, the plant will begin to wilt and soon dry up. The soil may still contain some moisture but the plant is unable to make use of it. The minimum moisture in the soil below which wilting will take place is called the Wilting Coefficient and at one time great importance was attached to it. The determination of this Coefficient, however, is tedius. One must grow a number of plants and slowly watch them wilt and determine the moisture content at that point. Moisture content of the soil is determined by heating a weighed quantity of the sample in an oven kept at 100-110 ^oC. The moisture is expressed as percentage on the oven-dry soil.

Clouds play very important role on weather and climate. The formation of clouds takes place when water condenses in the sky. Clouds are visible accumulation of tiny water droplets and ice crystals in the Earth’s atmosphere. The moisture is always present in the atmosphere though it may not be visible. Clouds form when air becomes cooler until the water vapour present in it condenses to liquid form. The condensation over oceans occurs around solid particles like salt, dust, smoke etc. suspended in the atmosphere. The type of clouds depends upon temperature and wind. The clouds are classified based on physical appearance and the height at which clouds are formed (Fig. 7.1).

Term moisture stress is generally applied to the stomata opening and transpiration increases with time until they close due to high temperature.

5.1. Causes of moisture stress in plants

Main reason is extent of transpiration which is affected by leaf size and composition, size and distribution of stomata on leaf, atmospheric humidity, temperature, wind velocity and day length.

High atmospheric temperature due to intense sun and increased transpiration causes closure of stomata and wilting of leaves even, if soil moisture content is not limiting.

This deficit is made up during night due to decreased transpiration.

In plants, moisture content decreases either due to increased transpiration or reduction in absorption or both.

Abstract

Anantapur is the driest district of Andhra Pradesh and hence, agriculture conditions are very often precarious. Groundnut grows where other crops fail and thus it is the predominant crop of Anantapur district. Groundnut is grown in about 7.5 lakh ha in Anantapur district; however the average yields are low at 500 kg ha^-1. Among various available vegetation indices, normalised difference vegetation index (NDVI) is widely used for all reasons, which is a single numerical indicator of presence and condition of green vegetation. NDVI mapping at a regional scale helps to assess the spatial changes in the vigour of green vegetation and thus occurrence of any moisture stress. Climate tools have a great role in understanding the crop performance and estimating the yields. This study was taken up by using freely available MODIS data to understand NDVI in terms of abiotic stresses over Anantapur district and linking with the actual rainfall conditions, groundnut crop acreage and production. Results indicate that low groundnut yields in general could be related to NDVI-based stress measurements and rainfall quantum and distribution in the area however, with a few exceptions. It is hoped that by combining improved practices through IWM with climate-adapted crop varieties, rainfed farmers of Anantapur district can sustain their crop production under present climate variability and become resilient to future climate change.

Keywords: Climate, MODIS, NDVI, Anantapur, Groundnut, Integrated Watershed Management

Abstract

Nitrogen is the most essential nutrient for all plants and is also the major limiting factor in plant productivity. High yielding varieties of all crops are responsive to nitrogen but its ever-increasing use has shown detrimental impact on the environment along with extremely low use efficiency. Nitrogen use efficiency (NUE) in crop plants depends on various internal and external factors, which are dealt in detail. There has been a significant interest in genetic engineering of the crops to improve NUE. The use of biotechnological interventions by manipulating genes of the nitrogen utilization pathway to improve NUE has not been very successful. But transgenics/mutants with modified capacities for nitrate uptake, assimilation and remobilization have enhanced our understanding of the genetic control of NUE. In both cellular, and at whole plant level the mechanisms involved in N remobilization from the senescing organs towards the grain have recently gained importance but their understanding is still preliminary. Recent evidences have shown that grain filling during later stages of crop growth is supported by N recycling.

Introduction

Food supply is a major concern in the near future as the human population touches 9 billion in 2050 and therefore, more food has to be produced. This has to be achieved in the face of climate change amounting to constant pressure of biotic and abiotic stresses. However, Swaminathan (2007) opined that science and technology can play a very important role in stimulating and sustaining an evergreen revolution leading to long-term increases in productivity without associated ecological harm. Rice is considered as global grain since it is consumed by more than half of the world population. In India rice supplies calorie requirement to more than 70 per cent of its population. This is evident from the fact that rice is cultivated in all the 30 states and 5 union territories of the Indian subcontinent of diverse ecologies ranging from below sea level to mountainous regions. Though India tops in rice acreage (43.61 mha) its productivity of 3.19 t/ha (rough rice) is far below world average of 4.12 t/ha and that of Asia’s average of 4.19 t/ha (Shobharani et al., 2010). This low productivity is mainly due to unfavourable ecologies of the varied ecosystems which, rice encounters during critical stages of its growth. Salinity and submergence are major abiotic stresses, next to drought, limiting rice production in South East Asia including India.

In India nearly 8 mha are salt affected of which 2 mha are coastal saline and 3.4 mha are sodic (Singh, 1994). Rice is predominant crop in these areas especially in vast stretches of eastern and western coasts of India. The presence of salts, in excess to plant growth, in the soil solution makes the soil detrimental to crop growth. The source of the excess salt may be from soil or irrigation water. The content and composition of salts (ions) in tandem with types of soil and weather conditions prevailing during the crop period decides the severity of salt stress. Similarly, f lash floods or short-term submergence regularly affect rice growing areas in South and Southeast Asia. Even more favorable irrigated areas experience flooding problems during the monsoon season. It is also prevalent in rainfed lowlands where most of the present day rice varieties do not survive complete submergence for more than a week. In India 30% of the rice growing area is prone to such menace resulting in severe yield losses (Neeraja et al., 2007).

Molecular markers may be a short DNA sequence that flanks base pairs like SNP, a Single Nucleotude Polymorphism, small fragment or SSR Single Sequence Repeat or an extended site as in the case of microsatellites. Schematic representation of the SSR assay is shown in Fig. 1. Genetic markers have to be easily identified, linked to a specific locus and polymorphic. Molecular markers are subdivided into codominant monolocus and polyallelic markers and the second group contains dominant, polyallelic and biallelic markers. 

The diversity of the haloarchaea found within a crystallizer pond of a solar saltern located in Siridao and Ribandar, Goa, India was examined. Both cultured-based and culture independent methodology were employed which included  surface spreading of samples on different media containing 25% crude salt/NaCl, DNA extraction from sediment and water samples, amplification of 16S rRNA gene and DGGE.

ABSTRACT

Frankia is a gram-positive, microaerophilic, actinomycete that enters into symbiotic associations with a number of woody dicotyledonous plants called actinorhizal plants. Frankia can fix atmospheric nitrogen through such associations. The actinorhizal-Frankia association is beneficial for the environment, since it increases the fertility of soils. Extensive studies on the biochemical and molecular aspects helped in the classification of Frankia. PCR-RFLP studies of the 16S rRNA and the nif regions of Frankia revealed considerable information regarding the diversity of Frankia strains, isolated from different parts of the world. The completion of three whole genomes of Frankia has bolstered the study of the molecular organization of the genome. Comparative genomics study of the three complete genomes revealed that, the strains reflected the biogeographical history of the host plants they infected. Striking similarity in the codon usage patterns highlights the fact that they may have evolved as a unit. In the present review we highlighted the taxonomy of Frankia and the host plants, the Frankia-actinorhizal association, regulation of infectivity, biochemical and molecular characterization, phylogeny and genomics of Frankia and stressed the need for metabolomic study of the Frankia-actinorhizal association.

Monitoring and evaluation constitute systematic processes of tracking, assessing, and learning from program implementation and outcomes to improve performance, demonstrate accountability, and inform decision-making. These complementary yet distinct functions serve as essential management tools that enable organizations and programs to understand whether they are achieving their intended objectives, efficiently using resources, and creating desired changes in target populations or systems. The integration of monitoring and evaluation creates powerful feedback loops that enhance program effectiveness while providing evidence for policy development, resource allocation, and strategic planning. Monitoring is defined as the systematic and continuous collection, analysis, and use of information about program implementation, progress toward objectives, and changes in context that may affect program performance. It represents an ongoing process that tracks inputs, activities,

Key words : GPS, GIS, DSS, SDSS, RS, Digital Elevation Model (DEM), Ground Water Exploration, Conjunctive Use of Surface and Ground Water, Digital Terrain Modeling (DTM).

Introduction

Water is one of the most precious gifts of nature to living beings and particularly to the mankind. On account of erratic distribution of rainfall both in time and space, the country is subjected to cycles of floods and droughts. With the increase in demand for water for various purposes and to mitigate the twin problems of floods and droughts faced by the country, a coordinated approach for optimum utilization and proper management of this precious resource is of prime importance. In addition, proper development and management of both irrigation and drainage systems are essential. This would require integrated management of surface water as well as gorund water. All the water related problems whether mitigating droughts or floods or better irrigation management practices, dictate for adoption of state of the art water management practices. Water management deals with the control, distribution and allocation of water flows and the treatment of rivers and catchments, consequently, solutions to many of the problems in water management require the knowledge and expertise from diverse sources.

Abstract

While harnessing the benefits of genetically modified (GM) crops, in terms of increased yield, better nutritional quality and resistance to biotic and abiotic stresses, it is important to undertake biosafety and risk assessment studies in a systematic manner to allay the concerns of their impact on the environment and especially on the genetic diversity. Since Bt cotton is the only commercialized GM crop in India, this paper reports on the monitoring of unintentional introgression of transgene(s)/ regulatory elements of four commercialized Bt cotton events, covering 99.5% of the area under cultivation, in 180 ex situ cotton accessions conserved in the National Gene Bank at National Bureau of Plant Genetic Resources (NBPGR), New Delhi, using polymerase chain reaction (PCR)-based assays. Based on the real-time event-specific and conventional transgene-specific PCR assays, no amplification signals specific to two major Bt cotton events MON531, MON15985; transgenes/markers/ regulatory elements of 4 widely cultivated commercialized Bt cotton events, i.e., cry1Ac, cry2Ab, marker genes, i.e., nptII (neomycin phosphotransferase), aadA (aminoglycoside-3’-adenyltransferase) and uidA (?-D-glucuronidase) marker genes, CaMV35S, nos (nopaline synthase) promoters and nos terminator were detected in any of the cotton accessions. The study shows that the cotton accessions conserved in National Gene Bank are GM free and the study can be used as a model for monitoring the adventitious presence of unintended mixing of cotton seed during seed production or harvesting in Bt cotton growing states of India. On similar lines, adventitious presence of transgenes can be checked in ex situ collections of different crops conserved in the genebanks.

Monitoring the microclimate inside protected cultivation systems—such as greenhouses, polyhouses, net houses, and other semi-controlled environments— plays a crucial role in modern agricultural practices that aim to enhance crop productivity, quality, and resource efficiency. As global demand for high-quality and high-yield crops continues to rise, protected agriculture has become a key solution for mitigating the effects of climate variability, pest infestations, and limited available land. However, the effectiveness of these systems largely depends on the ability to continuously monitor and regulate the essential microclimatic factors that directly influence plant health, disease resistance, and overall productivity. Microclimate refers to the localized climate conditions within a specific area— in this case, within the protected structure—that can differ significantly from external environmental conditions. Key parameters typically monitored include temperature, relative humidity, solar radiation or light intensity, carbon dioxide (CO?) concentration, vapor pressure deficit (VPD), and air movement or circulation. These factors are interrelated, and even minor fluctuations can greatly impact plant health, photosynthesis, transpiration, and reproduction. Thus, accurate and continuous monitoring of these variables is essential for effective climate control and optimal crop growth.
Temperature is arguably the most critical variable, as it controls metabolic processes, enzyme function, flowering time, and fruit development. A deviation from the ideal temperature range can either accelerate or stunt plant growth, leading to reduced yields or even crop failure. In tropical or semi-arid climates, temperature spikes inside protected structures can result in heat stress, while in temperate or mountainous regions, low temperatures can hinder growth. To mitigate this, temperature sensors—such as thermistors, RTDs, or thermocouples— are placed at different heights and zones within the structure to ensure uniform monitoring. These sensors typically transmit real-time data to centralized systems that control heating, cooling, and ventilation systems. Along with temperature,