eChapters

Horticultural crops include fruits, vegetables, flowers, plantation crops, spices, aromatic plants and medicinal species. They contribute substantially to nutrition, farm income, employment, exports and livelihood security. However, their productivity and quality are highly sensitive to temperature, rainfall, humidity, drought, flooding, salinity and other climate-related stresses. Changing climatic conditions affect flowering, fruit setting, maturity, nutritional composition, pest incidence and postharvest quality. Annual and perennial crops respond differently depending on their location, growth stage and environmental requirements. This chapter examines the influence of major climatic factors on horticultural production and discusses adaptive practices required to maintain productivity, quality and economic sustainability under changing environmental conditions.

Climate refers to the long-term pattern of temperature, rainfall, humidity, wind and other weather parameters within a geographical region. Human activities such as industrialization, urbanization, deforestation, land-use change and intensive agriculture have accelerated climate change by increasing greenhouse-gas emissions. Rising temperatures, irregular monsoon rainfall, droughts, floods, cyclones and elevated atmospheric carbon dioxide now affect crop growth, development and productivity. Different crops and developmental stages vary in their tolerance to these stresses. Consequently, farmers are modifying cropping patterns, sowing periods and crop choices. This chapter examines the effects of changing climatic conditions on major agricultural crops and identifies suitable adaptation and mitigation measures.

6.1 Nematodes as Biological Models
A model organism can be a non-human species (e.g., microbe, plant, or animal) with extensive genomic research data. These organisms are used to study and understand specific biological phenomena, with the expectation that discoveries made in these models will provide new insights into “big biological questions” (Semprucci et al., 2025). Model organisms are widely employed to explore potential causes and treatments for human diseases when direct experimentation on humans is unfeasible or ethically problematic. This approach is supported by the common descent of all living organisms, as well as the conservation of metabolic and developmental pathways and genetic material over the course of evolution. The use of model organisms is not confined to biomedical research. Significant advancements in developmental biology, eco-toxicology, fertilization processes, speciation, symbiosis, and sexual selection have been achieved using these models. Furthermore, emerging animal models—species with less neurological complexity than those protected by legislation—are increasingly recognized as a valid application of the Three Rs principle (Replacement, Reduction, and Refinement). To support this approach, databases of invertebrate species currently used or with potential applications in biomedical research are continually being developed and updated.
Nematodes, particularly Caenorhabditis elegans, are widely used as model organisms in biological research due to their simplicity, ease of study and shared biological processes with other animals, including humans. C. elegans, a small, free-living nematode, has a short life cycle, a known number of cells and a well-mapped genome, making it an ideal system for studying developmental biology, genetics and other aspects of animal biology.

5.1 Mass Multiplication Techniques
Entomopathogenic nematodes (EPNs) are currently produced by different methods either in vivo or in vitro (solid and liquid culture) (Friedman, 1990). Each approach has its advantages and disadvantages relative to production cost, technical know-how required, economy of scale, and product quality, and each approach has the potential to be improved. Following production, a variety of formulation and application alternatives are also available (Shapiro- Ilan et al., 2012). Mass multiplication of EPNs can be achieved through in vivo (using insect hosts) and in vitro (using artificial media) methods, with the latter being more efficient for large-scale production.
1. In Vivo Multiplication
Method: Involves rearing insect hosts (like Galleria mellonella or Corcyra cephalonica) and infecting them with EPNs, allowing the nematodes to multiply within the host’s cadaver.
Process
• Rearing of insect hosts.
• Inoculation of host larvae with EPNs.
• Collection of dead, infected larvae.
• Extraction of infective juveniles (IJs) from the cadavers using methods like the White trap.
Advantages: Simpler and requires less capital outlay.
Disadvantages: Labor-intensive and may not be as efficient for large-scale production.

Entomopathogenic nematodes (EPNs) are obligate parasites that infect and kill insects. Their short life cycles, simple rearing requirements, and straightforward molecular manipulations render them ideal to study host– parasite interactions. Additionally, their efficacy in reducing herbivore damage in the field contributed to their use as biological control agents in agriculture (Xi Zhang et al., 2021). EPNs comprise three genera, Heterorhabditis, Steinernema, and Oscheius. Infective juveniles (IJs) are third-stage free-living nematode larvae (iL3) that locate, select, and infect a host by entering through a natural orifice or by penetrating through the cuticle. Upon infection, IJs release venom proteins and regurgitate an endosymbiotic entomopathogenic bacterium, leading to a fatal toxemia and septicemia of the host. Juveniles then undergo a transition from free-living to parasitic lifestyle and resume growth and development by feeding on the infected flesh. Adult nematodes reproduce inside the host generating several new generations of nematodes. Resource depletion and elevated nematode densities induce the production of ascaroside C11 ethanolamine, an ascaroside triggering the production of next generation IJs, often through Endotokia matricida. The newly hatched IJs emerge from the resource-depleted host and search to infect new hosts.
Juvenile host-seeking strategies are typically classified along a gradient ranging from ambushing to cruising (Xi Zhang et al., 2021). Ambusher nematodes are stationary and infect mobile hosts. Attachment to a mobile host can be achieved through nictation, which corresponds to the nematode standing on its tail, curling, and propelling itself in the air. Cruiser nematodes disperse in the soil and locate sedentary hosts. Nematodes with intermediate strategies can ambush or disperse depending on the soil matrix and host

Entomopathogenic / Entomophilic / Insecticidal/ Entomogenous nematodes can be found in the orders Aphelenchida, Ascaridida, Mermithida, Oxyurida, Rhabditida, Spirurida and Tylenchida. Phylogenetic relationships reveal that nematodes have established trophic associations with insects numerous times (Adams and Nguyen, 2008). Nematodes may have a proclivity (exaptation) for negotiating the natural defenses of insects and their associations with insects are tremendously fruitful (ecologically and evolutionarily).
3.1 Evolution of Parasitism in Insect-transmitted Plant Nematodes
Nematode-insect associations have evolved many times in the phylum Nematoda, but these lineages involve plant parasitism only in the Secernentean orders Aphelenchida and Tylenchida. In the Aphelenchida (Aphelenchoidoidea), Bursaphelenchus xylophilus (Pine wood nematode), B. cocophilus (Red ring or Coconut palm nematode) (Parasitaphelenchidae), and the many potential host-specific species of Schistonchus (fig nematodes) (Aphelenchoididae) nematode-insect interactions probably evolved independently from dauerforming, mycophagous ancestors that were phoretically transmitted to breeding sites of their insect hosts in plants (Giblin-Davis et al., 2003). Mycophagy probably gave rise to facultative or obligate plant-parasitism because of opportunities due to insect host switches or peculiarities in host behavior. In the Tylenchida, there is one significant radiation of insect-associated plant parasites involving Fergusobia nematodes (Fergusobiinae: Neotylenchidae) and Fergusonina (Fergusoninidae) flies as mutualists that gall myrtaceous plant buds or leaves. These dicyclic nematodes have different phases that are parasitic in either the insect or the plant hosts. The evolutionary origin of this association is unclear.

Nematodes and insects probably have influenced the shape of each other’s evolution from as early as the Devonian (409 million years ago). Over the course of their shared history, nematodes have established many different types of associations with insects.
2.1 Evolution and Types of Insect–Nematode Associations
The majority of nematodes associated with insects (Rhabditida, in particular) are not true parasites, but have established only phoretic relationships. These phoretic nematodes usually are referred to as insect “associates.” Nematodes in phoretic relationships with insects usually rely on their host to transport them to food resources or improve their chances of finding a mate (or both) (Adams and Nguyen, 2008). Bacterial feeding soil nematodes often are found associated with insect cadavers and incorrectly inferred to be contributing factors to insect pathology. However, it has been proposed that nematode parasitism in some groups may have been initiated by necrogenous nematodes. Early stages in the evolution of insect parasitism may have involved necrogeny (feeding and reproducing on the bacteria of decaying insect cadavers). The association with decaying environments or insect cadavers may have become successively more intimate, as the nematodes began to utilize other saprophagous insects for transportation to new sources of food. Eventually, the nematodes could have changed from being passengers to parasites, invading and feeding on the tissues of their hosts.
i. Facultative Parasitism: Nematodes that are facultative parasites can infect a healthy insect and receive nourishment at the expense of its host, yet retain the ability to complete its life cycle independent of its

1.1 Nematodes in Agriculture
Nematodes offer several ecosystem services that affect the nitrogen cycle, decomposition capacity, and control of pests within soil systems. Firstly, the effects on the nitrogen cycle have generated a growing interest in the beneficial effects of nematodes to improve soil nitrogen content. Specifically, soil disruption, during activities such as tillage, increases nitrogen availability as well as the abundance of bacteria that compete for nitrogen (Ducker James, 2021). In response to more bacteria, bacterial feeding nematodes also increase in abundance, and this stabilizes nitrogen availability and increases nitrogen mineralization in soil once again, which is key for crop growth and soil fertility. Secondly, nematodes are involved in the decomposition of organic matter in the soil. Free-living nematodes are particularly essential as they recycle nutrients in the soil, which is matched by the indirect effect of bacteria and fungi-feeding nematodes that feed on the bacteria and fungi which decompose organic matter. Together, the feeding activity of these nematodes accelerates the decomposition process and makes minerals and nutrients accessible to growing plant roots.
Thirdly, nematodes are valuable biological agents of pest control. Predatory nematodes regulate populations of other organisms in soil as they feed on organisms such as protozoa, insects, and other nematode species. As a result, nematodes moderate the population of other nematodes, and studies have shown that nematodes such as the Steinernematidae and Heterorhabditidae can kill soil-based insect pests within 48 hours. Finally, nematodes have become key biological indicators of soil health. The diversity and richness of nematodes communities are particularly insightful as they act as proxies to reveal information on the levels of nutrients, fungal availability, and bacteria,

6.1 Introduction
The success of design thinking depends on its applicability in industries and service organization. The applications of design thinking applied to product development should focus on the needs of users. The design thinking can also be applied to design new processes as well as improve an existing process.
6.2 Case Studies
The section presents several case studies of applying design thinking in realities as listed below.
1. Design thinking in eye care
2. Design thinking in foundry
3. Design thinking in FMG company
4. Design thinking in auto industry
5. Design thinking at retail outlet
6. Design Thinking in Flight Reservation System
7. Design thinking in winding fabric on roller in textile mill
6.2.1 Case study: Design Thinking in Eye Care
Eye care is a crucial part of health care system. There are many factors that are responsible for poor eye vision. Normally in the early stages, viz. children and adults up to 40 or 45 years of age may have eye power issues, which are managed

5.1 Introduction
One of the main methods for cost avoidance and prevention is value engineering/ value analysis. “Society of American Value Engineers (SAVE)” states that value Analysis is the systematic use of known procedures which determine the function of a product or service, define a monetary value for the function, and provide the essential function reliably at the lowest overall cost. If these are applied to a new product, it is called value engineering. By analysing the function of any product, material part, component, system, or service, it is possible to find extraneous expenditures connected with them and effectively remove them without compromising the product’s quality, functional dependability, or ability to provide services. The principles and steps of value analysis/ value engineering will supplement the effort of design thinking.
5.2 Symptoms for Introducing Value Analysis (VA)
If one or more of the following symptoms exist, starting a VA programme is expected to yield very positive effects.
i. Sales of the company’s products are declining.
ii. The prices of the products of the company are greater than those of the products of its rivals.

4.1 Introduction
Companies that produce tangible items or offer services must drastically increase their productivity in order to maintain their operations in the face of intensifying global competition. A single quantum jump productivity enhancement technique or multiple concurrent incremental improvement productivity strategies can both be used to achieve this goal. If a business chooses the second choice, integrating various tactics for a significant increase in the total productivity would be quite difficult. The first alternative, a single productivity improvement plan, will make it possible for the business to increase productivity with the same or greater success. The only difficulty with this strategy is locating the probable problem area and then using process improvement techniques to achieve the anticipated dramatic increase in productivity. Business process reengineering refers to any significant adjustment of business processes that results in a dramatic increase in an organization’s productivity. Price, quality, after-sales support, adherence to delivery schedules, technological breakthroughs, etc. are some elements that influence market competition. Therefore, the business should focus its efforts on each of these areas in order to achieve notable improvements in terms of decreased operating costs and higher sales revenue from the sale of its goods and services to clients. Improved pricing for a company’s goods or services can provide it an advantage over competitions. Applying various cost-cutting strategies to lower variable costs and overhead costs will lower the price of the good or service. The other elements, viz. quality, after-sales support, delivery schedule adherence, and technological advancements are all directed in this direction

3.1 Introduction
Since the dawn of humanity, the expansion of invention and technological innovation has made it possible to make a variety of goods to meet its needs. To get a competitive edge in the market, the manufacture of a product has three main goals, viz. efficient product planning, efficient manufacturing, and effective cost control.
These can be achieved through a random thinking, if the designer is fortunate to have the best design of a product of interest, which satisfies the needs of its customers. But it will rarely happen. Design thinking maximizes the possibility of happening of the best design in the journey of design a product in shorter possible time.
Design thinking, an emerging field in this direction, tries to create a product by utilizing the following steps.
i. Empathize
ii. Define
iii. Ideate
iv. Prototype
v. Test
3.2 Definition of Design Thinking
A design methodology called “Design Thinking” offers a problem-based approach to problem solving. By understanding the human needs which changes quickly, re-framing the issue in human-centric ways, generating a lot of ideas during brainstorming sessions, and taking a hands-on approach during prototyping and testing, design thinking is incredibly helpful in handling complicated challenges that are ill-defined or unknown. Anyone can use the Design Thinking approach to tackle complex problems that arise in our society, such as those in our businesses, governments, and other spheres of influence, if they have an understanding of these five steps of the process.

2.1 Introduction
As time passes, the customers are used to the practice of having short life for the products, which they buy and use. This is because of the globalization of business and growing global competition all over the world. The companies engaged in manufacturing and selling the products which are used at large volume by households and partly by industries, introduce new models to attract customers and to have more market share. This in turn has impacts on customer buying and retention decisions of those products. Though the companies introduce new models, they are expected to keep the prices of the products at affordable ranges so that there is always a larger customer segment to buy those products. This is like egg or chicken situation, because companies introduce many new models to out beat competition and in turn customers expect improved versions of those models in shorter time intervals. This forces companies to continuously engage themselves to come out with new models or improved models which have convergence of technologies to give maximal customer satisfaction. So, creativity in product design becomes an essential requirement of the product development teams of the companies.
2.2 Product Design
Product design aims to include the following tasks to solve the problems of the users or address expected needs of customers in a given market. These tasks are human intensive.
i. Understanding end-users’ requirements
ii. Imagining alternate product ideas to address their needs

1.1 Introduction
The concept of invention is the fundamental requirement for design thinking. Since the birth of mankind, people started inventing many concepts, which are mainly observations of facts and innovation of ideas for producing products that are useful to the society. The design thinking requires unconventional thinking to design processes for maximal operational efficiency of manufacturing the products, which give maximum satisfaction to customers with more value for money. Hence, the team that is involved in the design thinking projects may gain the required skills by knowing several inventions of the past. Hence, a review of inventions is presented in this chapter.
1.2 Tracing Inventions
The content at https://www.explainthatstuff.com/timeline.html gives a complete account of inventions starting from 4–5 billion years ago up to 2022. The article on solar cell energy gives an account of the sun started producing energy. About 1.2 million years ago, humans invented fire, which has been further innovated for biofuels, candles, IC engines, aviation engines, etc. In the 7th century B.C., ants were killed by burning in the heat produced by concentrated sun rays using magnifying lens. Greeks and Romans utilised burning mirrors to light candles during religious ceremonies in the third century B.C. About 3.5 million years ago, stone, wood, antlers, and bones were used by humans to make first kind of tools. These were further improved to make tools for machines. About 4000 BCE back, iron was used to make decorative ornaments. About 3500 BCE, people invented the concept of wheel, which brought quantum revolution in transportation.

Introduction
Good Laboratory Practice (GLP) has been a cornerstone of laboratory operations, ensuring quality, reliability, and reproducibility in research and development across various fields, including pharmaceuticals, biotechnology, and environmental science. As we move further into the 21st century, the landscape of GLP is evolving rapidly, influenced by technological advancements, regulatory changes, and the increasing complexity of scientific research. This article explores future trends and innovations in GLP, delving into key areas such as automation, data management, regulatory shifts, and sustainable practices.
1. The Role of Automation in GLP
1.1 Increased Efficiency and Accuracy
One of the most significant trends in GLP is the integration of automation. Laboratory automation includes the prompt use of technology to perform tasks in a much efficient way with minimal human interventions, thus significantly increasing efficiency and accuracy of laboratory practices. Automated systems can handle repetitive tasks such as liquid handling, sample preparation, and data analysis, reducing the potential for human error.
Benefits of Automation
• Reduction of Human Error: Automation minimizes the chances of mistakes that can occur when humans perform these repetitive tasks, thereby improving the reliability of the results.
• Speed up the Research Process: For example, robotic systems can be used in drug discovery. These systems can conduct high-throughput screening, which means they can quickly test thousands of different compounds for potential effectiveness as drugs.

1. Introduction
 Laboratories form the backbone of research, clinical diagnostics and industrial quality control. Good Laboratory Practices (GLP) serve as the foundation for ensuring the quality, integrity, and reliability of scientific research, particularly in different pharmaceuticals drug testing, environmental studies and chemical testing. Good Laboratory Practices (GLP) in animal husbandry and dairy research play a crucial role in ensuring the accuracy and reproducibility of scientific studies related to livestock management, dairy production animal health and promoting animal welfare. Originally developed to prevent fraud and ensure regulatory compliance, GLP has evolved in response to advancements in technology, increased regulatory demands and the growing human and animals need for sustainability in laboratory operations. Laboratory settings are inherently fluid, with regulatory frameworks and technological progress constantly reshaping the environment. Studies indicate that even minor lapses in quality control can trigger significant inconsistencies in test results, outcomes that can have serious repercussions in both clinical and research domains. Therefore, cultivating a philosophy of ongoing improvement is crucial for addressing these evolving challenges, meeting the rising demand for safe, superior dairy products and embracing innovation. This article, synthesize the latest research findings and guidelines, delve into trusted best practices and outline practical strategies for embedding continuous improvement into everyday laboratory operations. A study published in the Journal of Laboratory Quality demonstrated that the adoption of structured quality indicators significantly minimized error rates and bolstered patient safety in clinical laboratories. Their research also provided a comparative analysis of different continuous improvement strategies, including the Plan-Do-Check-Act (PDCA) cycle and Lean Six Sigma, highlighting their respective benefits in lab environments. Based on these insights, the authors advocate for a comprehensive quality management system that seamlessly integrates automation, comprehensive staff training and routine audits to enhance process efficiency. Ultimately, the findings underscore that continuous improvement is an enduring journey, one that evolves along with advances in science, technology and regulatory standards.