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Nanoparticle characterization is necessary to establish understanding and control of nanoparticle synthesis and applications. Characterization is done by using a variety of different techniques, mainly drawn from materials science. Common techniques are electron microscopy (TEM, SEM), atomic force microscopy (AFM), dynamic light scattering (DLS), x-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF),ultraviolet-visible spectroscopy and dual polarisation interferometry.

Whilst the theory has been known for over a century (see Robert Brown), the technologyfor Nanoparticle trackinganalysis (NTA) allows direct tracking of the Brownian motion and thismethodtherefore allows the sizing of individual nanoparticles in solution.

Isolation of Protoplasts

Protoplast is the living material of the cell however an isolated protoplast is the cell from which the  cell wall is removed. In plant breeding programme many desirable combination of characters could not be transmitted through the conventional method of genetic manipulation. Higher plants that could lead to the genetic process involving fusion between the subsequent developments of a product to a hybrid plant is known as somatic hybridization. Plant protoplasts can be isolated from cells by two methods:

Data Mining is the process of automatic discovery of novel and understandable models and patterns from large amounts of data. Bioinformatics is the science of storing, analyzing and utilizing information from biological data such as sequences, molecules, gene expressions, and pathways. Development of novel data mining methods will play a fundamental role in understanding these rapidly expanding sources of biological data. Data mining techniques are an automated means of reducing the complexity of data in large bioinformatics databases and of discovering meaningful and useful patterns and relationships in data. Bioinformatics, the application of computational techniques to analyze the information associated with biomolecules on a large-scale, has now firmly established itself as a discipline in molecular biology, and encompasses a wide range of subject areas from structural biology, genomics to gene expression studies. It provides an introduction and overview of the current state of the field. The main principles that underpin bioinformatics analyses look at the Data Mining in Bioinformatics and databases that are commonly used, and finally examine some of the studies that are being conducted, particularly with reference to transcription regulatory systems.

Experiment - 1

Aim: Isolation of plant genomic DNA through modified CTAB method

Principle: Genomic DNA is extracted from plant material requires cell lysis, inactivation of cellular nucleases and separation of the desired genomic DNA from cellular debris. Ideal lysis method is rigorous enough to disrupt the complex starting material (plant tissue), yet gentle enough to preserve the target nucleic acid. The cetyltrimethylammonium bromide (CTAB) procedure is sufficient for the extraction and purification of DNA from plants and plant derived food stuff.  This is particularly suitable for the elimination of polysaccharides and polyphenolic compounds otherwise affecting the DNA purity and hence quality.

Experiment- 1

Aim: Direct DNA delivery to Plant by Particle Bombardment
 
In direct gene transfer method, the foreign gene of interest is delivered into the host plant cell without the help of vector. Klein and Coworkers (1987) evolved a procedure through which the delivery of DNA into cells of intact plant organs or cultured cells is done by a process called Projectile Bombardment. The microprojection (Small and high density particles) are accelerated to high velocity by particle gun apperatus. These particles with very high kinetic energy penetrate the cells and membranes and transmit foreign desired DNA inside of the bombardment cells.

Experiment - 1
 
Aim: Molecular analysis of putative transformed plants by Polymerase Chain Reaction

The expression of transgenes are not identified at that time but it can be achieved through DNA analysis of that plant. The presence of transgene are generally identified of PCR-based detection followed by gel electrophoresis and comparison with standard samples. The main achievement of PCR-based detection is that it is very sensitive. The Polymerase Chain Reaction (PCR) provides a rapid and sensitive method to amplify a specific gene sequence. 

1.     Rules and Regulation for Safety in a Microbiology Laboratory
    
2.     Cleaning of Glass Wares
    
3.     Sterilization

The history of nanotechnology in the modern world dates back to 1959 when American physicist Richard Feynman inspired the scientific community in his lecture “There is a plenty of rooms at the bottom”. Research on nanosized particles dramatically developed into an important field of modern research with potential effects in electronics to medicine and recently in agriculture and food industry.

Nanoparticles exhibit unique properties in terms of chemical, physical, photoelectrochemical and electronic properties when compared to their respective bulk materials. The applications of these nanosized particles are defined in a bigger ways in the field of Biotechnology. All these applications require custom based synthesis methods for specific applications.

Raman Spectroscopy

It is a spectroscopic technique based on inelastic scattering of monochromatic light, usually from a laser source. Inelastic scattering means that the frequency of photons in monochromatic light changes upon interaction with a sample. Photons of the laser light are absorbed by the sample and then reemitted. Frequency of the reemitted photons is shifted up or down in comparison with original monochromatic frequency, which is called the Raman effect. This shift provides information about vibrational, rotational and other low frequency transitions in molecules. Raman spectroscopy can be used to study solid, liquid and gaseous samples.

Genetic architecture of a population is the result of prolonged natural selection. The populations which exist in diverse environments might have been strongly diversified genetically. So, it is necessary to understand the extent and rates of genetic divergence existing between the diversified forms or types. Genetic diversity must be sufficient enough to justify breeding effort. Genetic diversity may be available in improved germplasm or it may exist only in genetically inferior stocks. The availability of statistical tools to quantitatively measure the genetic divergence between two or more populations and the relative contribution of individual characters to the total divergence have permitted to trace the evolutionary patterns in some crops such as rice and tobacco and in choosing the parents for hybridization in crop plants (Rao, 1958; Blackith, 1960; Morishima and Oka, 1960; Murthy et al.,1962). Among the several statistical methods developed for measuring the divergence between population, multivariate analysis (D² statistic) developed by Mahalanobis in 1936, has been found to be potent tool.

Mahalanobis D² analysis is an effective tool in quantifying the degree of divergence at genetic level and provides a quantitative measures of the association between geographic and genetic diversity based on generalized distance.

Intellectual property : Refers to creations of the mind: inventions, literary and artistic works, and symbols, names, images, and designs.

1. ICAR-Central Island Agricultural Research Institute, Port Blair

2. ICAR-Central Arid Zone Research Institute, Jodhpur

3. ICAR-Central Avian Research Institute, Izatnagar

All India Coordinated Research Projects

1. AICRP on Nematodes, New Delhi

2. AICRP on Maize, New Delhi

3. AICRP Rice, Hyderabad

4. AICRP on Chickpea, Kanpur

Agricultural University

17.1 Central Agricultural Universities

1. Central Agricultural University, Imphal Manipur

2. Rani Laxmi Bai Central Agricultural University, Jhansi Uttar Pradesh

3. Dr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur)

Unique Achievement in Plant Breeding

Rice

TN-1: First dwarf variety of rice developed in world

IR-8: Second dwarf and first high yielding variety of rice developed in world

Jaya: First dwarf variety of rice developed in India

Enterobacteria phage ? is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E.coli). It was discovered by Esther Lederberg in 1950. The wild type of this virus has a temperate life cycle that allows it to either reside within the genome of its host through lysogeny or enter into a lytic phase, during which it kills and lyses the cell to produce offspring. Lambda strains, mutated at specific sites, are unable to lysogenize cells; instead, they grow and enter the lytic cycle after superinfecting an already lysogenized cell.

Objectives

• To study working principles of simple microscope
• Study different parts of simple microscope

Introduction

This is the first ever microscope. It was founded by Leeuwenhoek in 17^th century. He combined a convex lens with a specimen holder, for seeing the magnified images of small objects. It looks like a magnifying glass because it has the capacity to magnify between 200 and 300 times. A simple microscope is a small focal lens used to view the magnified images of small objects. It is just like a hand lens and glass with relatively lower magnification power.

This method has been first described by H. H. Lowe in 1927. This is the most widely used method to handle segregating generations following crosses between two parents to develop new cultivars combining desirable traits from both the parents. In this method, superior types are selected in successive segregating generations and a record is maintained of all parent-progeny relationships. The records help the breeder to advance only progeny lines with plants that exhibit genes for the desired traits. Selection begins in F2 generation where individual plants are selected which in judgment of breeder will produce the best progeny in F5/F6 when the selection is finally concluded. In F3 and F4 generations, many loci will have become homozygous and family characteristics begin to appear. By F5/F6 generation, most families are expected to be homozygous and uniform and hence selection within such families is no longer effective. At this stage selection emphasis shifts to the selection among families rather than within families.

Selection of parents is of great significance for the success of this method. Normally, this method is used to replace already established variety. Therefore, one parent should be selected on the basis of proven performance in the particular area where the breeding is in progress. The second parent should be such that it complements the first parent for the character under improvement. Sometimes, three or four parents may also be used to supply the desired character by way of three-way or double crosses.

The application of back-cross breeding first proposed by H. V. Harlan and M. N. Pope in 1922 provides a precise way of improving varieties that excel in a large number of attributes but are deficient in a few key characteristics. F. N. Briggs too started an extensive back-crossing programme to develop bunt resistant varieties of wheat in 1922. As the name implies, the back-cross method of breeding makes use of a series of back-crosses to the variety to be improved during which the character or characters in which improvement is sought is maintained by selection. At the end of back-crossing, the gene (or genes) being transferred will be heterozygous. Selfing at the end of last back- cross produces homozygosity for this gene pair and coupled with selection will result in a variety similar to the well adapted and recurrent parent but superior for the particular characteristic for which improvement programme was initiated.

In back-cross method of breeding, the adapted and highly desirable parent is called the recurrent parent and the source of desirable character being transferred is called as donor parent. It is most effective and easy to apply when the missing trait is qualitatively inherited, dominant and produces a phenotype that is readily observed in a hybrid plant. The procedure for transferring a recessive trait is similar to that for dominant trait but involves an additional step of selfing. Back-crossing is also used to transfer entire sets of chromosomes in a foreign cytoplasm to create a cytoplasmic male sterile (CMS) genotype as has been practiced in maize, onion and wheat, etc. This is done by crossing the donor (of chromosomes) as male until all donor chromosome are recovered in the cytoplasm of the recurrent parent. Back-cross breeding has also been used to develop isogenic lines (lines that differ only in alleles at a specific locus) for traits like disease resistance and plant height, etc.

Mangelsdorf (1951) has opined that hybrid corn has been the most far-reaching development in applied biology. Hybrid cultivars have contributed enormously to world’s food and feed resources. Although dramatically successful at first in corn, hybrids have spread to several other crops both in cross-pollinated and self-pollinated species. Their use is likely to spread more in time to come. Hybrid varieties are those in which F1 populations are used to produce commercial crop. Parents of F1 may be inbred lines, clones, varieties, or other populations. Hybrid breeding is historically rooted in development of hybrids in corn and therefore most of the issues covered in this section relate to hybrid breeding in maize.