eChapters

Definition of terminologies and concept

Variables: Variables are the characteristic, number or quantity which increases or decreases over time and takes different values in different situations. Variables are those, whose value varies according to the situations. For example, maize yield, Stover, fertilizer, labor, land, pesticides, etc.
 
Dependent variable: Variables whose value is dependent on independent variables. These variables take different values only in response to independent variables. Yield of maize is dependent on amount fertilizer, fertilizer application methods, labor, pesticides etc. So in this case maize yield is dependent variables while amount fertilizer, fertilizer application methods, labor, pesticides are independent variables. However in other situation, maize yield can be used as independent variables also.

PLANT NUTRITION

Plants use inorganic minerals for nutrition, whether grown in the field or in a container. Complex interactions involving weathering of rock minerals, decaying organic matter, animals, and microbes take place to form inorganic minerals in soil. Roots absorb mineral nutrients as ions in soil water. Many factors influence nutrient uptake for plants. Ions can be readily available to roots or could be “tied up” by other elements or the soil itself. Soil too high in pH (alkaline) or too low (acid) makes minerals unavailable to plants.

Crop growth rate (g m^_-2 day^_-1)

Crop Growth Rate is referred to a whole crop, so you need to consider the area of the ground where crop is present. It is measured as mass increase in crop biomass per unit ground area per unit time.
Equation for that is, CGR= W₂-W₁ / t₁-t₂

Biotechnology has the potential to revolutionize many aspects in our lives, from healthcare to agriculture. However, along with its benefits, it also poses significant risks to human health, environment, and ecology. Therefore, it is essential to establish biosafety regulations and risk assessment frameworks to promote safe and responsible use of biotechnology. This chapter compiled an overview of biotechnology, the importance of biosafety regulations and risk assessment, IPR protection, contaminants, risk analysis, risk management in laboratories and fields, biosafety concerns to human health, environment and ecology, biosafety at international and national levels, bioethics, and general principles.

Overview of Biotechnology and its Potential Benefits and Risks

Biotechnology refers to the use of living organisms or their components to develop products and processes for various fields such as healthcare, agriculture, and environmental remediation. Biotechnology applications include genetic engineering, bioremediation, biopharmaceuticals, and biobased products.

Objectives

• Better understand the dihybrid cross.
• Calculation of dihybrid phenotypic, genotypic and testcross ratios.

Principle

A cross is made between two true-breeding parents differing in two pairs of genes, producing an F₁ generation. These plants are inter-crossed to produce an F₂ generation. Mendel second law i.e., Law of independent assortment can be explained by studying the inheritance of two characters at a time, simultaneously. When two phenotypically distinct parents differing with respect to two characters governed by two pair of genes are crossed together. Each pair of genes assorts independently into all possible combination forming four kinds of progeny, two of these are parental type and two are recombinant types.

Introduction

Restriction enzymes, recognizes specific base sequences in double-helical DNA and cleaves at specific places, both strands of a duplex containing the recognized sequences. To biochemists, these exquisitely precise scalpels are marvelous gifts of nature. They are indispensable for analyzing chromosome structure, sequencing very long DNA molecules, isolating genes, and creating new DNA molecules that can be cloned. Werner Arber and Hamilton Smith discovered restriction enzymes, and Daniel Nathans pioneered their use in the late 1960s.

Objectives

• Better understand the phenomenon of multiple alleles.

Introduction

The word allele is a general term to denote the alternative forms of a gene or contrasting gene pair that denote the alternative form of a gene is called allele. These alleles were previously considered by Bateson as hypothetical partner in Mendelian segregation. Mendel and his followers used the term ‘allele’ or ‘alleleomorph’ to denote the alternative form of the normal gene. It means the genes for tall and dwarf characters of pea plant are alleles. The former is normal or wild allele and the later is mutant allele. A gene can mutate several times producing several alternative expressions. When three or more alleles are found for any particular gene, these are called ‘multiple alleles’. These occupy the same locus in homologous chromosomes.

Multiple alleles are defined as genes that are members of the same gene pair and are located on the same locus. All of them control the same character but each of the allele affects that character somewhat differently than the others. In any diploid organism, a particular gene pair is represented by two alleles of the series, one allele on each of the two chromosomes of a homologous pair. This is because an organism has just two chromosomes of each type. These form a homologous pair. However, in the individuals of a population or species, many alternative forms of the same gene may be present. The grouping of all the possible alleles of a gene pair is defined as a system of multiple alleles and their mode of inheritance is called multiple allelism. The multiple allele system can be represented as A1, A2, A3, A4 etc.

Agarose gel electrophoresis is the easiest and commonest way of separating, capable of resolving mixtures of DNA fragment that cannot be separated conveniently by other procedures. The purpose of the gel might be to look at the DNA, to quantify it or to isolate a particular band. The DNA is visualised in the gel by addition of ethidium bromide. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it absorbs invisible UV light and transmits the energy as visible orange light.

Agarose, which is extracted from seaweed, is a linear polymer whose basic structure is shown in figure.

India has a unique social and cultural setting with diverse people, culture and religions. It is one of the oldest and greatest civilizations of the world. Geographically India extends snow clad Himalayan mountains in the north to the tropical rain forests of the south. It is the 7th largest country in the world and well recognised in Asia by its mountains, seas, deserts, and vast stretch of fertile plain land, which give the country a distinct geographical identity.

India has a very rich, diverse religious and cultural heritage. The four main religions of India are - Hinduism, Islam, Christianity and Sikhism. Hinduism is the largest religion in India, with approximately 80% of the population is Hindu while Islam (Muslim) is the second-largest religion having 14% (approx.) share.

Religion in India is characterised by the diversity of religious beliefs and practices. In Indian history, religion has been an important part of the country's culture. Indian subcontinent is the birthplace of four of the world's major religions, namely, Buddhism, Hinduism, Jainism and Sikhism, which are collectively known as native Indian religions and represent 83% of the total population of India approximately.

Hinduism

Hinduism is the main Indian religion. It is considered as a religious and universal order, a way of life which followed by the people who believed in Hinduism. The word ‘Hindu’ refers group of particular people who believes and follow Hinduism, the oldest religion in the world. It has also been described as ‘Sanatana dharma’.

The recent concept of intellectual property emerged in England in the 17^th & 18^th centuries. The term Intellectual Property came in use in the 19^th century and in the late 20^th century. As of now the Intellectual Property has occupied common position in the global legal systems. There are different forms of Intellectual property rights which include patents, copyright, industrial design, trademarks, plant variety rights, trade dress, geographical indications and in some jurisdictions trade secrets too

Major International Treaties Related to IPR & TRIPS

There are different forms of intellectual property like Patents, Copyright, Trademarks, Industrial design, Plant Varieties etc. Various meeting and conventions were also convened for the protection in these intellectual properties at international level from time to time and to bring uniformity and coordination among member countries. These were reflected in different treaties, which are mentioned below. Among all these treaties/conventions the agreement on Trade related aspects of intellectual property rights (TRIPS), which runs under aegis of World Trade Organization is most influential, comprehensive and inclusive of all.

Principle

Monomeric acrylamide (which is neurotoxic) is polymerised in the presence of free radicals to form polyacrylamide. The free radicles are provided by ammonium persulphate and stabilised by TEMED (N’N’N’N’-tetramethylethylene-diamine). The chains of polyacrylamide are cross-linked by the addition of methylenebisacrylamide to form a gel whose porosity is determined by the length of chains and the degree of crosslinking. The chain length is proportional to the acrylamide concentration: usually between 3.5 and 20%. Cross-linking bisacrylamide is usually added at a ratio of 2g BIS 38g acrylamide.

Self-Incompatibility

This is lack of self-fruitfulness and is a condition where the pollen from a flower is not receptive on the stigma of the same flower resulting into no seed setting. This happens despite the fact that pollen and ovule development both are normal and viable. This is caused by genetically controlled physiological hindrance to self-fertilization. This occurs widely among diverse families of flowering plants including the Leguminosae, Onagraceae, Rosaceae, Scrophulariaceae, Solanaceae, Compositae, Cruciferae, Papaveraceae and the Gramineae (now called as Poaceae). The incompatibility reaction is genetically governed by a single locus designated as S with multiple alleles whose number can go more than 100 in some species such as Trifolium pretense. East has estimated that this occurs in more than 3000 plant species distributed over 20 families. Lewis classified incompatibility systems into two main groups, namely, heteromorphic and homomorphic and homomorphic system has further been divided into gametophytic and sporophytic types as illustrated below:

Principle

The SDS polyacrylamide gel electrophoresis (PAGE) performs:

Separation of proteins according to their molecular weight

Ascertain whether a given protein is actually present in a sample,

Assessment of purity of the preparation

Estimation of the approximate quantity of the protein, and

Measurement of the size of the protein.

X-ray diffraction (XRD) is an important experimental tool used in determining crustal structure of material system in bulk and nanoregimes. Therefore, it is important to understand the basic concepts of XRD.

Heterosis or hybrid vigour is the condition where an F1 hybrid falls outside the range of the parents with respect to some character or characters. It is also defined as a manifestation of heterozygosity, expressed as increased vigour, size, fruitfulness and resistance to diseases, insects or climate extremes. It has been observed in nearly every crop including both self and cross-pollinated crops. It is converse of inbreeding depression. However, even the species that show little or no inbreeding depression, often benefit from crossing and resultant hybrids. Therefore, heterosis has been more emphasized than the inbreeding depression. It is defined in two basic ways:

Any phenotype is the result of interaction of genotype and the environment. Any genotype cannot express itself unless proper environment for its expression is made available. Likewise, environment cannot create any phenotype unless a genotype is there to be acted upon by the environment. However, the effect of genotype and the environment is not fixed, its keeps on changing depending upon the relative role of the genotype. Heritability therefore specifies the proportion of the total phenotypic variability that is due to genetic causes. In other words, heritability is defined as the ratio of genetic variance to the total phenotypic variance. Heritability is the degree to which the characteristics of a plant are repeated and expressed in the progeny. This can be expressed quantitatively as follows:

Heritability (h²) = Genotypic variance/Phenotypic variance

Principle

Western blot analysis (also called protein immunoblot) is a useful tool for the detection of proteins in biological samples. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose), where they are probed (detected) using antibodies specific to the target protein.  It is a technique for analyzing and identifying protein antigens: the proteins are separated by electrophoresis in polyacrylamide gel, then transferred (“blotted”) onto a nitrocellulose membrane or treated paper, where they bind in the same pattern as they formed in the gel. The antigen is overlaid first with antibody, then with anti-immunoglobulin or protein A labeled with radioisotope, fluorescent dye, or enzyme. 

The method originated from the laboratory of George Stark at Stanford. The name “Western blot” was given to the technique by W. Neal Burnette and is a play on the name “Southern blot”, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blotting and the detection of posttranslational modification of protein is termed “Eastern blotting”.

The International Union for the Protection of New Varieties of Plants or UPOV is an intergovernmental organization with headquarters in Geneva, Switzerland. UPOV was established by the International Convention. The objective of the Convention is the protection of new varieties of plants by an intellectual property right. The Convention was adopted in Paris in 1961 and revised in 1972, 1978 and 1991. UPOV aims to encourage the development of new varieties of plants for the benefit of society by codifying intellectual property for plant breeders.

The International Union for the Protection of New Varieties of Plants (UPOV) based in Geneva, Switzerland and established in 1961 is an intergovernmental organization to provide and promote an effective system of plant variety protection, with the aim of encouraging the development of new varieties of plants, for the benefit of society. For plant breeders' rights to be granted, the new variety must meet four criteria under the rules established by UPOV as discussed earlier also.