eChapters

In this chapter we will look at the Expert system which can guide and assist an individual once it is provided with the enough Knowledge to do so. Here we will discuss about expert system and its architecture with proper steps of making an expert system in any field by several tools which are available to do so. For expert systems the most important is Knowledge Engineering which is the collection of Knowledge from an expert and Engineering it in such a form that a machine could understand it.

Introduction

One of the pioneering fields of AI study is Expert Systems (ES). It is proposed by researchers in the computer science department of Stanford University. An expert system is a computer system which emulates a human expert’s decision-making skills. Expert systems are developed by reasoning through information structures, representing laws, rather than through traditional procedural code, in order to overcome complex problems.

Expert Programs use professional expertise systematically to address human expert problems. An expert is an individual with experience in a specific field. The expert has expertise or special credentials which most people do not learn or care about. An expert can solve or solve problems more effectively than people can solve. Expert System actually emulates all these qualities of expert and solve the problem in a better way and fast.

Today, Expert System (ES) is used in the fields of finance, research, engineering, technology, medicine and many others where there is a well-defined problem area. The fundamental principle is that an expert method should also define the steps to illustrate why a problem can be solved. Unless an individual can justify his reasoning for Expert System.

Several substrates, like proteins (enzymes), are very sensitive to alteration in the concentration of the solution they are placed in. Hence is the importance of a buffer which controls the chemical properties of the medium. Literally a “buffer” has the ability to resist changes in the hydrogen ion concentration. Although, buffers do contain other molecules, that might, in addition to the hydrogen ion concentration, influence the ionic strength, the activity of enzymes, and other parameters of the experiment.

In any biological experiment, appropriate buffer must be selected based on their effect on the experiment. Ideal buffers control pH and ionic strength without interacting adversely with the experiment undertaken. For example, although phosphate is a common buffer, it may not be suited when phosphate is a substrate or product of the reaction being studied. Also, some proteins interact poorly with some buffers. For example, Tris is not ideal as it exhibit high pKa and the large change in pKa when temperature changes, although it is cheap and most proteins are stable in Tris buffers, moreover it seldom reacts with reaction substrates hence, Tris buffer is commonly used in biochemical experiments.

Molecular biology labo-ratories are becoming very common in every University and research institutes and a good laboratory manners is extremely important as many of the equipments are very expensive and sensitive. Hence familiarity with equipments becomes very crucial before starting any experiment.

The Laboratory Worker is Surrounded by Many Dangers such as from..........

Handling of infectious materials.

Handling of broken glassware.

Accidental spill of corrosive reagents.

Swallowing of corrosive reagents such as concentrated Sulphuric acid,

Hydrochloric acid, sodium hydroxide, trichloroacetic acid etc.

Swallowing of infectious specimen.

Inhalation of poisonous fumes.

Infection in the conduct of necropsies.

Potential hazards in the form of inflammable chemicals and gas leakages.

Introduction
According to the U.S. Food and Drug Administration (FDA), Good Laboratory Practices (GLP) is a quality system that ensures the nonclinical safety studies are appropriately designed, conducted, and documented to generate reliable data. The laboratory techniques are defined as the processes and practices that are recommended for equipment use in the laboratory, while laboratory methods are based on established scientific principles involving biology, chemistry, and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol in blood to analysing DNA to growing microscopic organisms that may be causing an infection. One of the most significant parts of laboratory techniques and laboratory methods is the proper validation of the equipments (old or new equipments) as well as all the testing procedures/ methods (established or newly developed methods). Analytical method validation is a critical process to ensure the quality and safety of products. The objective of the validation of an analytical method is to demonstrate that it is suitable for its intended purpose; the results are authentic and universally acceptable. An analytical method is suitably well considered when the results generated through different method are consistent, reproducible, and thus, reliable.
The validation is required when
• A new method has been developed
• Revision of established method
• Use of established methods in different laboratories and different analysts, etc.
• Comparison of methods required
• When quality control indicates method changes

Summary

Lac is a resinous exudation from the body of female scale insect Laccifer lacca Kerr. belongs to family Lacciferidae of phylum Arthropoda, class  Insecta and order Hemiptera. The lac insect lives on native trees in India, China,Thiland, Burma/Myanmar and Malaysia. In India it is chiefly grown on trees like Kusum, “Palas”, and “Ber. It is also purposely cultured on shoots of these tree species. Lac cultivation is an alternative agricultural profession and provides subsidiary source of income for a large number of farmers mainly in Jharkhand, Chhattisgarh, West Bengal, Odisha, North-East states and other parts of the country. India, which is the highest producer of lac, contributes around 55% of the total world requirement followed by China and Thiland. The country exports around 80-90% of its production.

Year    Contributor    Description
1756    Duharmel du Monceau H.L.    Discovered callus formation in decorticated elm tree. 
1839    Schwann, T.H.    Proposed the view that each living cell of a multicellular organism would be capable of developing independently if provided with proper external conditions.
1853    Trecul, A.    Performed experiment on callus formation by decorticated trees such as Robinia, Pawlonia and Ulmus.

A cereal is any grass cultivated for the edible components of its grain (botanically, a type of fruit called a caryopsis), composed of the endosperm, germ, and bran. The term may also refer to the resulting grain itself (specifically “cereal grain”). Cereal grain crops are grown in greater quantities and provide more food energy worldwide than any other type of crop and are therefore staple crops. Edible grains from other plant families, such as buckwheat, quinoa and chia, are referred to as pseudo cereals. In their natural, unprocessed, whole grain form, cereals are a rich source of vitamins, minerals, carbohydrates, fats, oils, and protein. When processed by the removal of the bran and germ the remaining endosperm is mostly carbohydrate. In some developing countries, grain in the form of rice, wheat, millet, maize etc. constitutes a majority of daily sustenance. 

Sugarcane, (Saccharum officinarum), perennial grass crop of the family Poaceae, primarily cultivated for its juice from which sugar is processed. Most of the world’s sugarcane is grown in subtropical and tropical areas. The plant is also grown for biofuel production, especially in Brazil, as the canes can be used directly to produce ethyl alcohol (ethanol). The by-products from cane sugar processing, namely the straw and bagasse (cane fibres), can be used to produce cellulosic ethanol, a second-generation biofuel. Other sugarcane products include molasses, rum, and cachaça (a Brazilian alcohol), and the plant itself can be used as thatch and as livestock fodder.

Oilseed crops have been grown all over the world and are considered important crops due to their economic value. Oilseed crops are primarily grown for edible oil. Recently, oilseeds attracted more attention due to an increasing demand for their healthy vegetable oils, livestock feeds, pharmaceuticals, biofuels, and other oleochemical industrial uses. The increased interest resulted in an 82% expansion of oilseed crop cultivation areas and about a 240% increase in total world production over the last 30 years.

Pulses are high in protein, fibre, and various vitamins, provide amino acids, and are hearty crops. They are most popular in developing countries, but are increasingly becoming recognized as an excellent part of a healthy diet throughout the world. Pulse crops are one of the most sustainable crops a farmer can grow. It takes just 163 litres of water to produce 0.45 kg (1 pound) of pulses, compared with 818 litres for soybeans. They also contribute to soil fertility by fixing nitrogen in the soil.

In this chapter, we explore Latin square design (LSD), a specialized form of experimental design renowned for its efficiency in controlling variation. This chapter thoroughly discovers the foundational principles, structure, and application of LSD in experimental research. It details mathematical models for LSD and guides readers through the interpretation of ANOVA tables. Furthermore, the chapter provides a systematic approach to conducting LSD analysis using R software.

Latin Square Design

The LSD is a statistical design used extensively in experiments, especially when specific conditions are met:

• Controlled variation in two directions: LSD is particularly suited for experiments where variation can be controlled along two different factors. This design structures the experimental units into a square matrix, allowing two sources of variability to be accounted for.

• Adherence to experimental design principles: Like other designs, LSD follows the key principles of randomization, replication, and local control. Randomization in LSD is applied within the rows and columns of the square matrix, ensuring unbiased treatment comparisons.

• Efficient use of resources: LSD allows for an effective utilization of resources by controlling two variables simultaneously. This makes it a more efficient design compared to RBD or CRD when there are two sources of nuisance variables.

I. MANDATE OF COPYRIGHT LAW

The importance of intellectual property rights has arisen in recent years to a great extent. The law of copyrights protects original forms of expression i.e. Novels, Movies, Musical compositions and computer software etc. Earlier it was mainly confined to the -published Work and owner of it could prevent others from getting it published in the form of books or articles in order to get benefit in terms of money or goodwill or reputation. In case of such undue enrichment, the owner had a valid cause of action. The owner of the copyright has a valid cause of action against the violator. At present even in case of its adoption and translation or abridgement, it is obligatory to have consent of author. The main purpose and object of copyright law is to protect the expression in any other form from unauthorized use. Since 1847, the copyrights law is in developing stage so, many legislations has been enacted.1 With the changing of times, the meaning of expression also changes and the style of exhibit the things also changed. The right of author who gives expression in any form is protected from unauthorized use.

Formation of a Rectangle

Experiments are usually laid out in square or rectangular plots for data and yield estimation, even though the field may be irregular. The plot is usually placed on the contour, parallel with a road, or in a north-and-south alignment. To mark a plot, construct a right angle and then form a rectangle or square.

As we have seen how artificial Intelligence can be useful in agriculture but the nest question always come in mind is that from where and how we can start to learn and what are the model of learning an artificial Intelligence. As Artificial Intelligence is a huge part of Computer Science which means we need an language as medium to perform it and we always prefer Python for that which is very dynamic and easy to learn. In this chapter we will learn about python basics so that in further implementation of artificial Intelligence it will be helpful to us.

I. Introduction

Python is a remarkable language that can claim to be easy and strong. You will be amazed to see how simple it is not to focus on the language you are programming, but on solving the problem. Guido van Rossum was the creator of the Python language which is named after the BBC show “Monty Python’s flying Circus”.

Python is formally introduced as:

“Python is an easy to learn, powerful programming language. It has efficient high-level data structures and a simple but effective approach to object-oriented programming. Python’s elegant syntax and dynamic typing, together with its interpreted nature, make it an ideal language for scripting and rapid application development in many areas on most platforms.”

I. ROLE OF INTELLECTUAL PROPERTY

The international community has set itself the target of reducing the proportion of people below poverty line to half by 2015, along with associated specific targets for improving health and education and environmental sustainability. The Millennium Development Goals, adopted at various international fora, recognize the crucial importance of reducing poverty and hunger, improving health and education, and ensuring environmental sustainability. It is important to know whether and how intellectual property rights (IPRs) could play a role in helping the world meet these targets — in particular by reducing poverty, helping to combat diseases, enhancing access to education and contributing to sustainable development, protecting our cultural heritage, safeguarding the food security for the country. It is also necessary to deliberate whether and how they create obstacles to meeting those targets and, if so, how those obstacles can be removed.

There are strong views from both sides on the protection and enforcement of IPRs though there is no data to check these views objectively. Some argue strongly that IPRs are necessary to stimulate economic growth, which in turn, helps in poverty reduction. By stimulating invention and new technologies, IPRs help to increase agricultural or industrial production, promote domestic and foreign investment, facilitate technology transfer and improve the availability of medicines necessary to combat diseases. They are also of the view that there is no reason why a system that has worked for developed countries would not work for developing countries. Others argue equally vehemently the opposite. IPRs do little to stimulate invention in developing countries, because the necessary human and technical capacity may be absent there. In assessing these opposing arguments, it is important to remember the technological disparity between developed and developing countries as a group. What role the intellectual property can play to plug this gap is a serious subject for every developing nation? For this, it is also pertinent to know about the IPRs, the challenges they pose to the legal system in general and to the Indian legal system in particular, and their basic rationale to take a considered approach in their implementation.

8.1 Key legal statutes and regulatory foundations
Various policy measures were also undertaken by the Government of India in due course of time. The Government of India enacted four science policies after gaining independence, i.e. Scientific Policy Resolution (1958), Technology Policy Statement (1983), Science and Technology Policy (2003) and Science Technology and Innovation Policy (2013). The 1983 Technology Policy Statement possessed a simple goal of creating indigenous technology and boosting efficient absorption and adaptation of imported technologies suited to the nation’s priorities and. The Technology Policy Statement of 1983 was followed up through the establishment of the Technology Information Forecasting and Assessment Council (TIFAC), an autonomous organization entirely owned by the Government of India under the Department of Science and Technology during 1988. TIFAC also offers some funding and supports development and commercialization of technologies through the home-grown technology scheme, among its other operations. The Technology Development Board Act (1995) was also a significant policy move of the government in the same direction. Also in recent times, the Government of India also unveiled its own National IPR Policy 2016 with the motto cum slogan “Creative India, Innovative India”. In addition, the policy is aligned to a number of other schemes of the Indian Government like “Make in India”, “Atal Innovation Mission”, “Start-Up India” and “Stand-Up India”, all aimed at encouraging creativity, innovation and entrepreneurship in the nation.

Introduction

This led to an extended debate and international co-operation in the recognition of plant-related intellectual property rights, resulting in a greater attention being paid to questions of ownership of genetic resources. At the same time concerns for sustainable use of biological diversity were also raised.

One of the major events was the Convention on Biological Diversity (CBD) which came into force in 1993, which was adopted during the Rio Earth Summit of the United Nations. It was the first legally binding institutional mechanism, providing for conservation and sustainable use of all biological diversity and intends to establish the process of the equitable sharing of benefits arising out of the use of biodiversity.

The Convention on Biological Diversity

The CBD was conceived in the 1980s and was negotiated under the leadership of the United Nations Environment Programme (UNEP). It was adapted in June 1992 at Rio de Janeiro, at the Earth Summit, and is effective since December 1993.

Lentil (Lens culinaris, 2 n = 2 x = 14, family Fabaceae/Leguminosae, subfamily Papilionaceae, essentially self-pollinated annual) has average global area, production and yield during 2015-17 as 5.56 million ha, 6.55 million tons of production and 1178 kg/ha as per FAOSTAT. The top ten lentil producing countries globally are Canada, India, Turkey, USA, Nepal, Australia, Ethiopia, Bangladesh, China, and Kazakhstan. India produced 0.94 million tons of lentil from 1.60 million ha with a productivity of 587 kg/ha during 2010-11. The corresponding figures for the year 2018-19 are 1.56 million tons, 1.51 million ha and 1033 kg/ha indicating a significant gain in the productivity over the years (Directorate of Economics and Statistics, 2020). The top states in lentil production in India are Madhya Pradesh (40%), UP (30%), West Bengal (11%) and Bihar (9%). Rest of the states is insignificant. The plant was given the scientific name Lens culinaris in 1787 by Medikus, a German botanist and physician.