eChapters

The impact of nanotechnology in the food industry has become more apparent over the last few years with the organization of various conferences dedicated to the topic, initiation of consortia for better and safefood, along with increased coveragein themedia. Several companies which were hesitant about revealing their research programmes in nanofood, have now gone public announcing plans to improve existing products and develop new ones to maintain market dominance. The typesof application include:smartpackaging, on demand preservatives, and interactive foods. Building on the concept of “on-demand” food, the idea of interactive food is to allow consumers to modify food depending on their own nutritional needs or tastes. The concept is that thousands of nanocapsules containing flavour or colour enhancers, or added nutritional elements (such as vitamins), would remain dormant in the food and only be released when triggered by the consumer.24 Most of the food giants including Nestle, Kraft, Heinz, and Unilever supportspecific research programmes tocapturea share of the nanofood market in the next decade.

The definition of nanofood is that nanotechnology techniques or tools are used during cultivation, production, processing, or packaging of the food. It does not mean atomically modified foodor foodproduced by nanomachines. Although there are ambitious thoughts of creating molecularfood using nanomachines, this is unrealistic in the foreseeable future. Instead nanotechnologists are moreoptimisticabout thepotential to changethe existing systemof food processingand to ensure the safety of food products, creating a healthy food culture. They are also hopeful of enhancing the nutritional quality of food through selected additives and improvements to the way the body digests and absorbs food. Although some of these goals are further away, the food packaging industry already incorporates nanotechnology in products.

Introduction

Nature has been performing wonderful feats for millions of years. All organisms from microbes to humans are powered by highly evolved molecular and cellular machines that operate at nano scale. The capability to manipulate, control, assemble, functionalise, produce and manufacture things at atomic precision provides an opportunity to understand this new and exciting field of science. Nanotechnology is application of science to control the properties and structure of matter at nanometric scale.

Despite substantial advances in the diagnostics and treatment of diseases, parasitic infections are a major global health issue causing significant mortality and morbidity. Currently, chemotherapeutic antiprotozoal drugs, anthelmintic and anti-ectoparasitic agents are used to treat, but resistance to these drugs has developed due to overuse. In this scenario, nanoparticles are proving a major breakthrough in the treatment and control of parasitic diseases. In the last decade, there has been enormous development in the field of nano medicine for parasitic control. Gold and silver nanoparticles have shown promising results in the treatments of various types ofparasitic infections. These nanoparticles are synthesized through the use of various conventional and molecular technologies and have shown great efficacy. 

Agriculture serve as a backbone in improving economic growth of a country as well as it provides a base in meeting various needs of people in direct and indirect ways. The recent advancement in nanotechnology has shown a path to develop traditional agriculture into a sustainable practice. Several nanochemicals emerged as promising agents for better plant growth, used as fertilizer, pesticides, control weeds and also used as antimicrobial agents in food packaging. Nanotechnology may act as sensor for checking soil health of agriculture fields.

Nanotechnology is a multidisciplinary domain dedicated to manipulating materials at the nanometre scale (1-100 nanometres), where distinctive physical, chemical, and biological features arise. This transformative technology has applications across various sectors, including medicine, electronics, energy, agriculture, and environmental protection. The concept originated from Richard Feynman’s 1959 lecture, which envisioned the direct manipulation of atoms and molecules. Since then, advancements in microscopy and materials science have propelled nanotechnology into a significant area of applied science. In healthcare, it facilitates targeted drug delivery systems, improving therapeutic efficacy and reducing negative effects. Nanotechnology is also essential in ecological applications, including wastewater treatment and pollution management, where nanomaterials are employed for efficient filtration and remediation. Despite its potential, it raises ethical, safety, and regulatory concerns, including the long-term effects of nanoparticles on health and the environment. The lack of standardized regulations poses challenges for the safe deployment of nanotechnology in various applications. In agriculture, it facilitates the development of nanofertilizers and nano-pesticides, which improve nutrient delivery and reduce environmental impact. Recent research in India highlights the promising use of nano-bionoculants—nano-formulated bio-fertilizers that enhance plant growth and soil health by encapsulating beneficial microbes. Studies have shown that combining nanomaterials with bioinoculants can significantly improve crop yields and resilience to stress, indicating a shift towards sustainable agricultural practices. Nanotechnology represents a paradigm shift in science and technology. It offers innovative solutions to pressing global challenges while necessitating

Nanotechnology epitomizes one of the most dynamic and transformative fields in modern science and technology. This multidisciplinary domain bridges chemistry, physics, biology, along with engineering, enabling the creation of materials and devices with extraordinary properties and applications. The book chapter reflects the research and patenting trends in nanotechnology. USA leading the global share for most patents granted followed by South Korea, Japan, China and Germany for the top five spots contributing more than 76% of all the granted patents by EPO & USPTO related to nanotechnology. Nanotechnological products in relation to sub-industrial areas of medicine, food, agriculture, cosmetics, environment, and renewable energies are also highlighted in chapter. 

Nanotoxicology is the study of the toxicity of nanomaterials. Because of quantum size effects and large surface area, nanomaterials have unique properties compared with their larger counterparts

Nanotoxicology is that branch of bionanoscience, which deals with the study and application of toxicity of nanomaterials. The nanomaterials, even when they are made of inert elements like gold, become very active at a nanometer range. Nanotoxicological studies are intended to determine whether and to what extent these may pose a threat to the environment and to human beings. For instance, Diesel nanoparticles have been found to damage the cardiovascular system in a mouse model.

Human health and safety

Calls for tighter regulation of nanotechnology have occurred alongside a growing debate related to the human health and safety risks associated with nanotechnology. The Royal Society identifies the potential for nanoparticles to penetrate the skin, and recommend that the use of nanoparticles in cosmetics be conditional upon a favorable assessment by the relevant European Commission safety advisory committee. Andrew Maynard also reports that ‘certain nanoparticles may move easily into sensitive lung tissues after inhalation, and cause damage that can lead to chronic breathing problems’.

Historically, Indian scientists have recognized the importance of conserving plant biodiversity with particular reference to indigenous     and naturalized crops and their wild relatives. Indian scientists have recognized the importance of conserving plant biodiversity as early as 1935 by the ‘Crops and Soil Wing’ of the then Board of Agriculture and Animal Husbandry. Late Dr. B. P. Pal, in his classic paper ‘Search for New genes’ published in 1937 emphasized the importance of germplasm augmentation and utilization in crop improvement programmes. The need was reiterated in a meeting of the Indian Society of Genetics and Plant Breeding in 1941, which inter alia discussed the subject of economic crops. Dr. B. P. Pal, working at the then Imperial (now Indian) Agricultural Research Institute approached the then Imperial (now Indian) Council of Agricultural Research (ICAR) to set-up a unit for assembly of global germplasm under phytosanitary conditions in India. The ICAR scheme for plant introduction started functioning in 1946 under the leadership of late Dr. Harbhajan Singh as the first ‘Operational Scientist’. The scheme was further expanded and strengthened as ‘Plant Introduction and Exploration Organization’ in the Botany Division in 1956 and later established as a separate Division of Plant Introduction in IARI in 1961 headed by Dr. Harbhajan Singh.

The concepts of biosafety and biosecurity are central to efforts to protect human health against the risks posed by exposure to hazardous biological agents. Biosafety, a term used to describe the collection of technologies, processes and practices a term used to describe the collection of technologies, processes and practices aimed at preventing the unintentional exposure to biological agents, has in particularaccrued increasing importance in recent decades as a result of the trend towards globalization and concomitant growth in international communication, transport and trade (WHO, 2020). In this international context, the outbreaks of highly-infectious diseases that have occurred in the last years serve to underscore the urgent need for effective prevention and detection and response to biological risks, in accordance with the International Health Regulations (IHR).

Introduction

Indian Agriculture has made enormous strides in the past 50 years, raising foodgrains production from 50 million tonnes to over 200 million tonnes.  In the process, the country has progressed from a situation of food shortages and imports to one of surpluses and exports.  Having achieved food sufficiency, the aim now is to achieve food and nutritional security at the household level.

The increase in agricultural production, however, has brought in its wake, uneven development, across regions, crops, and also across different sections of farming community.  In the decade of the ‘nineties’, a marked slackening in the pace of growth has occurred, pointing to the need for infusing a new vitality in the agricultural sector.

ABSTRACT

The law of intellectual property has assumed utmost importance the World over, in the recent past and it is being considered as one of the most important subjects of law in view of its implications on economic, social and cultural realms. The term “intellectual property” has come to be internationally recognized as covering, patents, industrial designs, copyrights, trademarks, know-how and confidential information or trade secrets. This paper presents an overview on nature and scope of these elements of IPR.

Introduction

• Thus "genetic enhancement" or "pre-breeding" refers to the transfer or introgression of genes or gene combinations from unadapted sources into breeding materials (FAO, 1996).

• Genetic enhancement plays an important role in utilizing unadapted and unutilized germplasm collections and creating vast genetic variability for development of productive cultivars / hybrids.

• Objective of Genetic Enhancement is broadening the genetic base and increases genetic diversity

• Gene pool – Harlan and de Wet (1971).

• Primary gene pool (GP-1): Members of this gene pool are probably in the same "species" (in conventional biological usage) and can intermate freely.

• Secondary gene pool (GP-2): Members of this pool are probably normally classified as different species than the crop species under consideration (the primary gene pool).

• Tertiary gene pool (GP-3): Members of this gene pool are more distantly related to the members of the primary gene pool. The primary and tertiary gene pools can be intermated, but gene transfer between them is impossible without the use of "rather extreme or radical measures such as embryo rescue (or embryo culture, a form of plant organ culture) induced polyploidy (chromosome doubling) bridging crosses (e.g., with members of the secondary gene pool).

Nerium (Nerium oleander), commonly known as oleander or Kaner, is an evergreen flowering shrub cultivated for loose flowers, landscaping and religious uses. Its flowers are widely used for garlands and temple offerings, while the plant is valued in landscaping because of its drought tolerance and adaptability to poor soils. The chapter presents its origin, distribution, genetics, botanical description and important varieties. It explains climate and soil requirements, propagation, planting, nutrient management, irrigation, pruning and other cultural operations. Pest and disease management is also covered. Special attention is given to harvesting fully open flowers, postharvest handling and shelf-life improvement. The chapter also discusses yield and the plant’s toxic nature.

Northern Blot

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.

Introduction

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression levels during differentiation, morphogenesis, as well as abnormal or diseased conditions. Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence. The term ‘northern blot’ actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane; however the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.

DNA Isolation Protocol
Introduction

Most of the all procedures involved in molecular biology experiments require the purified form of nucleic acid. It forms a fundamental requirements for genome characterization, gene mapping and for identification and isolation of gene for genetic engineering. The most important step involved in removal of proteins which is carried out in different ways. The following protocol is one of the most commonly used protocols in animal, plant and microbial samples.

The search for the chemical basis of genetic material began about one and half century ago. Soon after discovery of Mendelian principles of segregation and independent assortment in 1865, Friedrich Miescher (1844-1895) published a method for separating cell nuclei from the cytoplasm and from these cell nuclei, he extracted an acid material termed as “nuclein” which had unusually large amount of phosphorous. Miescher concluded that this substance was unique and was just not comparable with any other group known at that point of time. Later on nuclein, now named as “nucleic acid” was found to be associated with various proteins and the combined entities were termed as “nucleoproteins”.

Procedure

Place a portion of your RNA sample (approximately 40 mg, hydrated) into a heavy walled Pyrex test tube. Add 1.0 ml of 1 N HCl and seal the tube.

Heat the tube in a boiling water bath for 1 hour.

Cool the tube, open it and place the contents into a centrifuge tube. Centrifuge the contents at 2,000 RPM in a clinical centrifuge to remove any insoluble residue. The supernatant contains your hydrolyzed RNA.

Prepare Whatman filter paper No. 1 for standard one-dimensional chromatography.

Using a micropipette, spot 20 µl of your hydrolyzate onto the paper, being careful to keep the spots as small as possible (repeated small drops are better than one large drop). Allow the spots to completely dry before proceeding.

Place the paper chromatogram into your chromatography tank and add the solvent (acetic acid:butanol:water). Allow the system to function for an appropriate time (approximately 36 hours for a 20 cm descending strip of Whatman #1). Remove the paper and dry it in a circulating air oven at 40° C for about 2 hours.

Locate the spots of nucleotides by their fluorescence under an ultra-violet light source. Expose the paper chromatogram to a UV light source and outline the spots using a light pencil. The order of migration from the point of origin is guanine (light blue fluorescence), adenine, cytilic acid and finally, uridylic acid.

After cafefully marking the spots, cut them out with cutters and place the paper cut outs into separately labeled 15 ml conical centrifuge tubes. Add 5.0 ml of 0.1 N HCl to each tube and allow the tubes to sit for several hours to elute the nucleotides from the paper.

A well-managed nursery is essential for producing healthy, uniform, vigorous, and field-ready planting material. This chapter explains nursery planning, site selection, propagation structures, growing media, containers, trays, irrigation, fertilization, sanitation, pest management, hardening, and transplant quality assessment. Protected raising techniques using shade nets, polyhouses, insect-proof structures, and controlled environments are discussed for improving germination and seedling survival. Particular attention is given to biosecurity, disease prevention, substrate sterilization, water quality, and environmental control. The chapter also introduces quality indicators such as root development, shoot growth, and physiological fitness. Efficient nursery management reduces establishment losses and provides the foundation for productive and profitable horticultural enterprises.

Maize occupies an important position in the world economy, and serves as an important source of food and feed (Gupta et al., 2015a). Together with rice and wheat, it provides at least 30% of the food calories to more than 4.5 billion people in 94 developing countries (Shiferaw et al., 2011). In India, maize is an important cereal too, and is grown on an area of 9.2 million hectares with production of 24.2 million tonnes (www.indiastat.com). The demand for cereals will continue to increase as a consequence of the expanding human population. The world will have around 7.7 billion people by 2020, and it will reach up to 9.3 billion by 2050, and the demand for maize between now and 2050 will double in the developing world (Rosegrant et al., 2009). By 2025, India too would require to double the production (50 million tonnes) of maize grain to meet the domestic demand (Yadav et al., 2016).

Malnutrition caused by inadequate consumption of balanced diet has emerged as one of the alarming problems especially in the under-developed and developing world. Mostly the resource-poor suffer from ‘hidden hunger’, a term often used to describe malnutrition caused due to micronutrient deficiencies in staple food diet (Bouis and Welch, 2010).

An estimated two billion people suffer from micronutrient deficiency, while 795 million people are undernourished (IFPRI, 2016a). Of the 667 million children under the age of five worldwide, 159 million are stunted, while 50 million do not weigh enough for their height (IFPRI, 2016b). An estimated 45% of deaths of children under age five are linked to malnutrition (Black et al., 2013). Malnutrition contributes to global burden of disease, and loss in annual gross domestic product (GDP) in Asia and Africa to extent of 11%. South Asia is home to more than 35% of the world’s poor, and 21.9% of the population of India lives in poverty, and thus vulnerable to various health problems (IFPRI, 2016a). 15.2% of the people in India are undernourished, and more than one third of the world’s underweight malnourished children live in India, posing severe socio-economic loss to the country.