eChapters

Introduction

Drought is one of the primary factors responsible for depressing rice yield in chronic areas and in destabilising rice production in drought-prone areas. It is a production constraint common to all rainfed rice cultures: wetland, upland, and deepwater (O’Toole and Chang 1979). Breeding for drought resistance, a major component of the International Rice Research Institute’s (IRRI) Genetic Evaluation and Utilization (GEU) Program since the early 1970s, aims at countering the adverse effects of drought in unfavoured rice production areas.

Rice germplasm has such remarkably rich diversity that a continuous spectrum of genotypes differing in the various physiological mechanisms can be found and is available for genetic manipulation (Chang et al. 1982a). However, effective evaluation methods are essential for unravelling the specific mechanism(s) involved and for devising eficient selection criteria in a breeding program. Moreover, the broad spread in growth duration among rice cultivars makes it impractical to obtain uniform evaluation of a large number of cultivars when they differ in physiological responses that are specific to certain growth stages. Methods that can accommodate large numbers are essential to breeding program.

Principle

The SDS polyacrylamide gel electrophoresis (PAGE) performs:

Separation of proteins according to their molecular weight

Ascertain whether a given protein is actually present in a sample,

Assessment of purity of the preparation

Estimation of the approximate quantity of the protein, and

Measurement of the size of the protein.

Introduction

Electrophoresis is the migration of charged molecules in solution in response to an electric field. Their rate of migration depends on the strength of the field; on the net charge, size and shape of the molecules and also on the ionic strength, viscosity and temperature of the medium in which the molecules are moving. As an analytical tool, electrophoresis is simple, rapid and highly sensitive. It is used analytically to study the properties of a single charged species, and as a separation technique. SDS-PAGE is the most widely used method for qualitatively analyzing any protein mixtures. The method is based on the separation of proteins according to their size and then locating by binding them to a dye. It is particularly useful for monitoring protein purity and determines their relative mass. The SDS-PAGE enables the students to understand the theory behind the techniques in checking the purity of the protein and identifying the protein in a test sample.

Separation of Proteins

Proteins are amphoteric compounds; their net charge therefore is determined by the pH of the medium in which they are suspended. In a solution with a pH above its isoelectric point, a protein has a net negative charge and migrates towards the anode in an electrical field. Below its isoelectric point, the protein is positively charged and migrates towards the cathode. The net charge carried by a protein is in addition independent of its size - i.e.: the charge carried per unit mass (or length, given proteins and nucleic acids are linear macromolecules) of molecule differs from protein to protein. At a given pH therefore, and under non-denaturing conditions, the electrophoretic separation of proteins is determined by both size and charge of the molecules.

Summary

Plants produce over 200,000 metabolites, which are important source of drugs and industrial materials. They have been traditionally classified as primary and secondary metabolites. The primary metabolites like sugars, amino acids and fatty acids that are needed for general growth and physiological development of plants are widely distributed in nature and are also utilized as food by man. Secondary metabolites are natural products, the absence of which does not result in immediate death, but in long term their impairment may lead to malfunction and reduced survivability. These plant secondary metabolites can be divided into groups that share the same core substructure, originated from the same biosynthetic pathways. Secondary metabolites play a major role in the adaptation of plants to the changing environment and in overcoming stress constraints. In this chapter we discussed the structure, properties, classification and functions of some of the important groups of plant secondary metabolites such as phenols, flavonoids, terpenoids and alkaloids. A brief overview of some of the important fungal secondary metabolites, their structures and importance has also been discussed. Biotechnological approaches which can be adopted to engineer plants for production of secondary metabolites have also been discussed.

Introduction

In today’s world, where environmental issues are prominent and there’s a growing realization of our planet’s limited resources, the concepts of sustainable harvesting and conservation have become extremely important. They act as guiding principles, directing us to use resources responsibly and to protect the diverse life and ecosystems on Earth. The paper is designed to explore the core principles of sustainable harvesting and conservation, emphasizing their critical importance and the pressing need for a careful equilibrium between utilizing resources and safeguarding the environment. The paper analyzes laws, policies, and delving into Sikkim’s sustainable conservation and harvesting methods, and argues that successful implementation of these strategies presents Sikkim as a model that can be replicated worldwide. Sikkim’s proactive initiatives, such as banning plastic and harmful substances, as well as implementing measures to reduce pollution, showcase a forward thinking approach to environmental stewardship(Sikkim, ND),. These actions set an example for other areas to emulate, promoting a more sustainable and responsible relationship with our natural surroundings.

Introduction

People visit tourist destinations for the purpose of enjoying the beauty, to relax and rejuvenate. One of the popular destinations chosen by all are hill stations. Darjeeling, also known as the Queen of Hills, is a popular tourist destination visited by six-hundred-thousand Indians and thirty-thousand foreigners annually (Bhattacharya, n.d.). The primary attractions that make Darjeeling worth visiting are the magnificent views of the Kanchenjunga range and the lush tea gardens. As a result of the advancement in technology, the luxury of travel is now feasible to all sections of society because of which tourism in India has rapidly expanded in the past three decades. This is the result of: great progress and advancement of the Indian economy, India’s roaring IT sector and promotion of “Unbelievable India” by the Tourism Ministry. Although tourism has significantly boosted revenue and employment, it comes with its downsides: the harm that it poses to the environment if it goes unchecked. Since the number of tourists is greater than the ability of the environment to cope up with, it puts tremendous pressure upon the environment which can have irreversible negative impacts

Introduction

Among the various countries worldwide, such as China, Kenya, and Sri Lanka, India stands as a significant player. In 2016, China and India collectively accounted for over 60% of the world’s total tea production. Furthermore, India holds the distinction of being the foremost producer of black tea, while China and Japan lead in green tea production. In India, Assam and Darjeeling are recognized as the primary states for tea production, while the Nilgiris and North-East regions also contribute significantly, playing a crucial role in boosting the country’s economic growth.

Assam annually produces over 400 million kilograms of tea, making it the largest tea-growing region globally.Assam has the world’s biggest tea plantations, which are important for India’s economy. The state is near the Brahmaputra River and shares borders with Bhutan, Bangladesh, and Myanmar. Because of its geographical location, this region encounters significant rainfall, especially during the monsoon season, with daily precipitation reaching as high as 250 to 300 mm (10 to 12 inches).A daytime temperature reaches to around 36 °C (96.8 °F), fostering greenhouse-like conditions of intense humidity and heat. The unique malty flavor for which Assam tea is renowned is shaped by the pivotal role played by its tropical climate.

Introduction

The term “green intellectual property” (IP) is used to describe the legal protections afforded to environmentally friendly innovations. It’s a strategy for saving the planet by stimulating development of environmentally friendly products and methods. For a very long time, plastic consumption has been a huge environmental problem. A report on single-use plastics has been released by the United Nations Environment Programme (UNEP), outlining steps towards environmental sustainability. This research analyses the efforts made at the national, state, and local levels to reduce the usage of disposable plastics. Policymakers considering restricting the manufacturing and use of single-use plastics may find the insights it presents helpful. In 2015, the United Nations General Assembly established the Sustainable Development Goals (SDGs) as part of the 2030 Agenda for Sustainable Development. By 2030, the world’s population should be free of poverty, the planet would be in good shape, and people would live in peace and prosperity. Several of these targets address plastic use; for example, 12: Responsible Consumption and Production, 13: Climate Action, 14: Life Below Water, and 15: Life on Land.

The Seed Act 1966 was passed by the Indian Parliament on 29th December 1966 and it came into force from 2nd October, 1969. In addition, Seed Regulation Order was also passed in December 1983.

Indian Council of Food and Agriculture organized SEED WORLD-2019 (World Seed Trade and Technology Congress) from 18-21 September, 2019 at The Hotel Lalit Ashok, Bangalore and presented SEED INDUSTRY SCENARIO REPORT (ICFA, 2019; www.icfa.org.inwww.seedworld.in). The information as given below is largely based on this report and other presentations made during the congress, particularly one by Kapur (2019).

Seed certification is a legal programme to maintain and make available to the public high quality seeds and planting material of genetically distinct crop varieties. Under seed production programme, quality seed is produced by govt. farms research stations, progressive farmers and seed producers using careful quality control, pedigreed plant stock, field inspections during the growing season and seed inspections after the harvest. Certification is an officially recognized method for maintaining varietal identity of the seed. In India certification is carried out by State Seed Certification Agencies.

After harvesting and threshing, the seed usually arrives at the cleaning plant with trash, green leaves, weeds, other crop seeds and insects, etc. If the seed contains leaves, etc. and other high moisture materials, it cannot be accurately cleaned and safely stored until most of the foreign material has been removed. The process of removing the unwanted materials from a seed lot is known as seed conditioning. Seed conditioning is an essential component of seed technology to ensure that the farmers get the seed of high quality.

Seed enhancement is the process or technology to further improve seed performances under very specific regimes and with certain planting equipment. Three major seed enhancement technologies are as follows (Copeland and McDonald, 1995). 
        Seed hydration 
        Biological seed treatments 
        Seed coatings

Seed is the most important component for productive agriculture. Significant advances have been made in production of food crops in India during the last four decades. The quantum jump in the food production primarily accounted for by increased productivity and production in rice and wheat was the result of introduction of improved varieties and their seeds often nicknamed as Green Revolution. The food production which was nearly 50 million tones during 1950 – 51 reached to a level of 210 million tones during 2003 – 04. During the same period the productivity increased about 3 folds (Table 20.1).

Seed quality is a multiple criterion that encompasses several important attributes. A seed scientist may be concerned with each quality characteristic of an individual seed while the seed trader normally considers the quality components of a seed lot. Any individual seed may possess certain measurable seed quality components which include:

Certified seed must meet certain seed standards in order to ensure an adequate level of seed purity along with the reasonable level of seed germination so that the farmer gets a good stand of the crop conforming to the varietal description. The components of the seed standard are as follows :
     The minimum percentage of pure seed 
     The maximum permissible limit for inert matter 
     Other crops seeds  
     Weed seeds 
     The maximum permissible limit for objectionable weeds 
     The maximum permissible limits of seeds of other distinguishable varieties (ODV) 
     Maximum permissible limits of seeds infected by seed bone diseases 
These standards are ensured in the seed crop by strictly adhering to field standards and careful harvesting, threshing, seed drying and then brining the same to the seed processing /seed conditioning plants. The Indian minimum seed standards for different crops are given in Tables 24.1-24.3 (Agarwal, 1995).

The purpose of seed storage is to preserve planting seed from one season  to the next. Further, the objective of seed storage is to maintain seed quality for the longest duration possible. 

Relative Humidity and Temperature Influence Seed Storability

The two most important factors that influence the storability of seed are relative humidity and temperature. The effects of relative humidity and its subsequent effect on seed moisture and the temperature of the storage environment are highly inter- dependent. Harrington (1963) gave two useful thumb rules for relative humidity and temperature in reference to storage life of the seed. These rules are as follows :

1. Nucleus seed: It is the handful of original seed obtained from selected individual plants of a particular variety. It has the highest genetic purity and physical purity.

Principle

Cloning experiments would be very difficult if we do not screen those very few cells that took up the recombinant plasmid DNA, as the transformation efficiency is very low as stated before (usually less than 1 in 10⁵ cells). Hence it is necessary that there should be a selection mechanism (i.e. conditions where cells grow if they express a gene, but die if they do not) to sort out which are transformed cells and which are not make the job easy.

A commonly used selection mechanism is antibiotic resistance. If the E. coli strain used is not resistant to the antibiotic, but the plasmid DNA contains a gene coding for resistance, then only the cells that have taken up the plasmid will be able to grow. Most commonly used plasmids contain a gene for resistance to ampicillin; only cells that have taken up the plasmid will be able to grow and form colonies.

Note: Uptake of plasmid DNA is rare; hence each group of cells visible on a plate represents the offspring of a single cell that will contain a single molecule of plasmid DNA.

Self incompatibility in plants is a mechanism preventing the seed set by self pollination, and is probably way in which out crossing is enforced. Other factors may cause selfed seed may not to set seed due to embryo abortion, but SI is prezygotic and prevents embryo formation. SI is, therefore prevention of fusion of fertile male and female gametes after fertilization. Koelreuter first time in 18^th century reported S.I in Verbascum phoeniceum plants. In self-incompatible pollen grains fail to germinate on the stigma of the flower that produced them. Self incompatibility has been reported in more than 300 plant species belonging to 70 family of angiosperm. It is important out breeding device (cross pollination) for normal fruit set. It is maintained high degree of heterozygosity and can take place at any stage between pollination and fertilization.