eChapters

Objectives

• Better understand the various types of gene interaction.

Introduction

Gene interaction is the phenomenon of two or more genes that affect each other’s expression in different ways in the development of a single character of an organism. It is the modification of normal phenotypic expression of gene due to interaction between alleles or non-alleles. In such situation, two or more than two genes may interact to give rise to a particular phenotype

Objectives

• Better understand the dihybrid cross.
• Calculation of dihybrid phenotypic, genotypic and testcross ratios.

Principle

A cross is made between two true-breeding parents differing in two pairs of genes, producing an F₁ generation. These plants are inter-crossed to produce an F₂ generation. Mendel second law i.e., Law of independent assortment can be explained by studying the inheritance of two characters at a time, simultaneously. When two phenotypically distinct parents differing with respect to two characters governed by two pair of genes are crossed together. Each pair of genes assorts independently into all possible combination forming four kinds of progeny, two of these are parental type and two are recombinant types.

Objectives

• Differentiate between fixatives and stains.
• Describe methods of staining.
• List the commonly used fixatives and stains and their composition.
• Describe the uses of above mentioned solutions and stains.

Introduction

Every time a part of an organism is removed, the action of bacteria will soon disintegrate, dry or putrefy it. They must be kept as close to normal as possible or in other words preserved and fixed in order to study these. Chemicals are required in many practical of Biology. Some of these chemicals preserve and specifically colour certain cell parts while others are used as solutions. You will learn about certain fixatives (preservatives) and stains in this chapter.

Objectives

• Find out the number and types of gametes and gametic recombination in monohybrid, dihybrid and trihybrid crosses.

Principle

Gametes are reproductive cells that join together to form a new cell called a zygote during sexual reproduction. Pollen in seed-bearing plants, is the gametophyte producing male gamete. Female gametes (ovules) are included in the ovary of plant. At maturity a sexually reproducing individuals forms the gametes. Homozygote generate only one form of gamete. Homozygous referring to a diploid cell or organism possessing two identical alleles of a specific gene (e.g. AA or aa). Heterozygote generate different types of gametes. Heterozygous referring to a diploid cell or organism possessing two different alleles of a specific gene (e.g. Aa).

Objectives

• Better understand the monohybrid cross.
• Calculation of monohybrid phenotypic, genotypic and testcross ratios.

Principle

Monohybrid cross deals with the study of inheritance of one pair of contrasting characters. The alleles of contrasting character selected in the monohybrid cross will show exact dominance and recessive relationship. It is also defined as a cross is made between two true-breeding parents differing for a single trait, producing an F₁ generation. These plants are intercrossed to produce an F₂ generation. Monohybrid crosses study led to the Mendel’s law of segregation. Monohybrid cross was first produced in pea by Mendel to explain the “law of segregation”. When parents with distinct phenotypes are crossed, in F₁ generation, only one type of plants appears phenotypically. However, segregation can be observed in F₂ progeny and the population can be divided into two phenotypes classes. After counting the individuals. It is observed that 75% of them express the dominant phenotype and 25% of them express recessive phenotype.

Objectives

• To study working principles of simple microscope
• Study different parts of simple microscope

Introduction

This is the first ever microscope. It was founded by Leeuwenhoek in 17^th century. He combined a convex lens with a specimen holder, for seeing the magnified images of small objects. It looks like a magnifying glass because it has the capacity to magnify between 200 and 300 times. A simple microscope is a small focal lens used to view the magnified images of small objects. It is just like a hand lens and glass with relatively lower magnification power.

Introduction
A study protocol tool is a very important comprehensive document that deliberately outlines the whole research rationale design and plan for the successful execution of the epidemiological/ an experimental/ a clinical study. It serves as a blueprint for how the study will be conducted, ensuring consistency, transparency, and adherence to ethical guidelines. The document contains the background information available in literatures on the basis of which the researchers plan to conduct the study with objectives that will answer research related questions with ensuring adherence to ethical standards.
Basic Elements of a Study Protocol
The study protocol includes different components including background Information available in literature for research plan, objectives or purpose of conducting the research, explanation of the study design, selection and exclusion of subjects, treatment of subjects, assessment of outcome safety and efficacy. The purpose of conducting research includes questions like why the study needs to be done and what will be its relevance to scientific society or environment. To achieve these objectives the researchers, design their research work for proper execution of research objectives. The study which is to be designed has structured framework for researchers to answer their research questions and obtain reliable and valid results. The study design not only helps the researcher in learning technical skills but also discovering the creative voice (planning and execution). The study design includes different methods, processes and necessary resources for accomplishment of research problems. The study design also incorporates the methods of data collection and statistical analysis which meets the requirements of desired outcome. The implementation of an appropriate study design guarantees a methodical and exacting research process, resulting in more precise findings and the appropriate type of analysis to address the research-related queries. This makes the conclusions more reliable and legitimate. By ensuring that the data are reliable, repeatable, and validated, a well-designed study contributes to the advancement of knowledge. Additionally, it helps to control biases and confounding variables that could affect the results and helps to clarify the methodology of the study, which

ABSTRACT

This paper presents few measures to be taken up by academic institutions, professional societies, industrial bodies and governmental agencies for the propagation of information of IPR in terms of encouragement of publication of books on IPR, establishment of IPR cells in academic and professional institutions and making IPR education as part of curriculum in Universities and institutions of higher learning.

Modern agriculture faces the challenge of increasing food production while minimizing environmental impacts like soil degradation, water pollution, and biodiversity loss. To address these issues, integrating microbial biotechnology with nanotechnology has emerged as a promising approach. Nanoparticles (NPs) with plants and microbes, enhances nutrient uptake, plant growth, and stress tolerance. Bioinoculants, which contain beneficial microorganisms, promote plant health, but are limited by stability and environmental factors. The synergistic use of nanoparticles and bioinoculant offers a novel solution, improving crop yield, nutrient uptake, and resilience to stress, reducing dependence on chemical inputs. This integration supports the development of more resilient, resource-efficient, and environment-friendly agricultural systems.

3.1 Introduction

The concept of nanoscience was first described in 1959 by physicist Richard P. Feynman in a lecture to the American Physical Society and it was Japanese researcher Norio Taniguchi who coined the term nanotechnology in 1974 to describe precision engineering with tolerances of a micron or less. Eric Drexler in 1986 in his book Engines of Creation, brought nanotechnology into the public domain.

Techniques have been developed to produce nanotubes in sizeable quantities, including arc discharge, laser ablation, high pressure carbon monoxide (HiPCO),andchemicalvapor deposition (CVD).Mostof these processes take place in vacuum or with process gases. CVD growth of CNTs can occur in vacuum or at atmospheric pressure. Large quantities of nanotubes can be synthesized by these methods; advances incatalysis and continuous growthprocessesare making CNTs more commercially viable.

Aim

To synthesize chitosan/nanosilica coating solution for fruit coating aiming at increasing shelf life.

Introduction

Application of edible coatings is promising to improve the quality and extend shelf life of fruits and vegetables (Lin et al., 2011). Chitosan, a versatile biopolymer derived from deacetylation of chitin had been widely applied in the fresh-keep field owing to its good biocompatibility, biodegradability, antibacterial activity and capacities to form film (Lin et al., 2011).

The most commonly used technique has been discussed and we have tried to generalize the type of particle that can be synthesized by each of these methods. Though it is not a rule that is applicable to all cases but it can be applied to most cases.

Polymerization Radical Polymerization

The polymerization could be a free radical polymerization or an atom transfer radical polymerization. The process of atom transfer radical polymerization (ATRP) is better than the free radical polymerization as control of molecular weight and size of the particle can be achieved. The coating of polymer on silica nanoparticles is generally done by ATRP. The surface of the silica particle is modified with a suitable initiator.

Aim

To synthesize iron (III) oxide (Fe₂O₃) nanoparticles

Introduction

Iron oxide nanoparticles have a number of applications in catalysis, sensors and high sensitive biomolecular magnetic resonance imaging (MRI) for medical diagnosis and therapeutics. They can be synthesize by the bottom-up approaches including the sol-gel technique.

Aim

To synthesize silver (Ag) nanoparticle.

Introduction

The Ag nanoparticles easily interact with other particles and increases their antibacterial efficiency. The Ag nanoparticles can be synthesized using various methods: biological, chemical electrochemical, γ-radiation, photochemical, laser ablation etc (Udapudi et al., 2012). The most popular preparation of Ag colloids is chemical reduction of Ag salts by sodium borohydride or sodium citrate (Udapudi et al., 2012).

16.1 Introduction

The stabilization of ultra fine as nano-particles in suspension is very important for both controlling the particle size and for developing process based application of these suspensions to achieve a desired result. Generally, two approaches, namely, Top Down and Bottom-Up approaches are followed for the preparation of ultra fine or nano particles. In the Top-Down method, production starts from the micro-scale and the particles size is reduced to nano-size through repeated impact and collision in high-energy ball mills. However, controlling of size, morphology, surface properties and electrostatic charge is often difficult. In the Bottom-up approach, production of nano-particles starts with the individual atoms and building up to a nano-structure by chemical processes.

Solid state materials can exists in various forms namely:

1. Single crystals (preferred for authentic characterization of structure and physical properties)
2. Polycrystalline powders that are agglomerates of large number of crystallites and are used for characterization when single crystal cannot be easily obtained
3. Nanocrystalline or nanostructured materials that posses large surface area
4. Thin films that can be grown in the best crystalline form for many applications
5. Amorphous systems that have no long-range translational order

Abstract

Synthetic biology is a multidisciplinary field of study that seeks to understand living systems and organisms, and utilizes engineering principles to design and create new biological components, devices, and systems, as well as modify existing natural systems. This chapter provides an introduction to synthetic biology and describes the procedures and methodologies involved in the design and construction of novel biological systems. The applications of synthetic biology are vast and include the development of biosensors, gene circuits, metabolic pathways, and genetically modified organisms for use in a variety of industries, including healthcare, agriculture, energy, and environmental remediation.

A variety produced by crossing inter se a number of genotypes selected for good general combining ability in all hybrid combinations with subsequent maintenance of the variety by open-pollination. According to Hayes and Garber (1919), the synthetic production of an improved variety by inbreeding and cross-breeding seems a reasonable plan. Synthetic cultivar is a commercial entity and a combination of number of inbred lines, sibbed lines, clones or other populations of cross-pollinated crops. The features of the components are as follows:

1. They will have been tested for their combining ability.
2. They will be preserved for future syntheses of the synthetic variety.
3. The component lines will be combined in a manner so as to allow random mating.

Synthetic varieties are normally used in cross-pollinated crops in those situations where hybrid development and organized seed production are in the preliminary stages. Inbred lines are common components of synthetics because they are easy to maintain and can be used again and again as per requirement of reconstituting the synthetics. When selfing is not possible, sibbing may be used. In case of perennials, clones are used as components. Combining ability of the component lines is determined in different ways. General combining ability is desired more than the specific combining ability because there are several components to a synthetic variety and each is required to combine well with the rest other lines. Random crossing of components of a synthetic variety is assumed and each line should contribute the same amount of seed to the next generation. Because a synthetic variety is a mixture of many genotypes, it might change under selection pressure during multiplication.

Summary

Synthetic seed technology is an alternative to traditional micropropagation for the production and delivery of cloned plantlets. Several aspects of the technique are still underdeveloped and hinder its commercial application. This technology may be of greater value in breeding programs and allows the propagation of many elite genotype-derived plants in a short time and would provide an easy and novel propagation system for the elite as well as difficult to root species. Also, encapsulation of propagules that were produced in vitro could reduce the cost of micropropagation of plantlets for commercialization and final delivery to the farmer field. This technology also employed for germplasm storage and exchange purposes.