eChapters

6.1 Introduction

Plant breeding is the indispensible component of elevating production and productivity of spices by evolving high yielding and/or with good quality type. Thereby, the practice of identification and selection of desirable traits and introgression of those characters in a single plant is being done since the beginning of organized agricultural activities by humankind. The act of plant breeding was reshaped and got a momentum with a scientific basis after the discovery and rather rediscovery of Mendel’s laws. Worldwide, humankind could enjoy the direct benefits of methodical plant breeding during green revolution. Manipulation of genetic architecture for creating variation in the population and successive selection is the basic of all kinds of crop breeding. But the process of creation of variability and selection differs from crop to crop depending on their mode of reproduction. It is comparatively easier to get a new homogeneous population in self pollinating plants. Therefore, segregating generations are handled differently in these two categories of self and cross pollinated crops.

Olericulture, a Latin word, is used to designate the science, culture and management of vegetable crops. In India, about more than fifty vegetable crops are grown in different parts, depending on their climatic requirement. A number of vegetables have similarity in their method of cultivation, which makes the package of practices followed by the farmers for their cultivation very complicated and brings about a lot of repetition. Whichever the method is adopted for the classification of vegetables can systematize the preparation and presentation of the material to some extent and can avoid the unnecessary duplication of some of the principles and methods of vegetable cultivation. In different vegetables, different plant parts are used as food in different forms and they belong to different botanical families with varying cultural and climatic requirements but common insect-pests and diseases along with their common control measures.

Various methods may be used to classify all the vegetable crops into different categories but no single method of classification would be useful to serve the purpose for different groups of scientists like taxonomists, botanists, physiologists, horticulturists, agronomists, and breeders. Under field conditions, the classification based on cultural practices has been proved the best adaptable and very useful for the growers. Vegetable crops can be classified in the following ways for the convenience of different groups of scientist and growers:

Olericulture, a Latin word, is used to designate the science, culture and management of vegetable crops. In India, about more than fifty vegetable crops are grown in different parts, depending on their climatic requirement. A number of vegetables have similarity in their method of cultivation, which makes the package of practices followed by the farmers for their cultivation very complicated and brings about a lot of repetition. Whichever the method is adopted for the classification of vegetables can systematize the preparation and presentation of the material to some extent and can avoid the unnecessary duplication of some of the principles and methods of vegetable cultivation. In different vegetables, different plant parts are used as food in different forms and they belong to different botanical families with varying cultural and climatic requirements but common insect-pests and diseases along with their common control measures.

Various methods may be used to classify all the vegetable crops into different categories but no single method of classification would be useful to serve the purpose for different groups of scientists like taxonomists, botanists, physiologists, horticulturists, agronomists, and breeders. Under field conditions, the classification based on cultural practices has been proved the best adaptable and very useful for the growers. Vegetable crops can be classified in the following ways for the convenience of different groups of scientist and growers:

Climate change exposes onion production to irregular rainfall, heat, drought, flooding and increasing pest and disease pressure. These stresses can reduce yield, damage bulb quality, increase storage losses and threaten smallholder livelihoods. This chapter uses research literature and case studies to examine resilience at farm, community and supply-system levels. It emphasizes location-specific varieties, crop rotation, micro-irrigation, efficient input use, digital forecasting, protected production, improved storage and reliable cold chains. Farmer organizations, extension services, subsidies and supportive institutions are also important. Sustainable onion farming requires agronomic, technological, socioeconomic and policy measures to function together, enabling growers to adapt to climatic uncertainty while conserving resources and maintaining profitability.

The importance of fruits and vegetables in human nutrition is well known. Vegetables are a rich and comparatively cheaper source of minerals and vitamins. Besides, their consumption in sufficient quantities provides taste and palatability, increases appetite and provides a fair amount of fibers. These are currently reckoned as an important adjunct for the maintenance of good health and beneficial in protecting against some degenerative diseases, thus, they form a vital part of human diet. Besides, vegetables now a day are being considered as an asset for providing a good source of income to the growers.

Vegetables originated in different parts of the world, some came from tropical or subtropical countries and others from temperate zones, some from humid areas and others from climates that are more arid. Most of the countries in the world are gifted with varied agro-climatic environments for growing an array of vegetable crops to combat the present imbalanced diet of a vast population of the world, particularly in the developing countries. Production of vegetables in developed countries is much higher, for instance in Japan, nearly 40% of the diet of the people is vegetables. To meet out the full dietary needs of the common man, to eliminate malnutrition, deficiency of minerals and vitamins and also to relieve the stress for increased production of cereals, there is a greater need for growing vegetables on larger scale. As such, for increased production of nearly 247 known vegetable crops, a sound knowledge of the various aspects of vegetable production technology, environmental stresses and constraints, techniques of raising, pre- and post-harvest technology, marketing and seed production techniques is of utmost importance. Breeding and selection of new cultivars have allowed for a greater adaptability to less favourable growing conditions than was possible in the past but the inherent agroclimatic requirements of a specific kind of vegetable have not changed materially. Time or season of planting of a particular vegetable crop is determined by its environmental requirement. Knowledge of specific agroclimatic conditions required for a particular crop could enable the grower to select the best season of planting in any particular area (Singh et al., 1991).

Clonal crops belong to at least 34 families including herbs, shrubs, trees and vines. Clonal crop improvement dates back to 10,000 years ago. The diversity of clonal crops and their wild relatives are very significant but not yet fully understood and exploited. Many crops represent combination of sexual and clonal reproduction. Different parts of the clonal crops which may or may not be the same as consumed parts have been shaped as clonal propagules such as stem, setts, tuber, rhizome, corms and bulbs among others.

Botanical description :Botanically, cluster bean is known as Cyamopsis tetragonoloba (L) Taub. (2n = 2x = 14) belongs to the family Papilionaceae.

Floral biology :Cluster bean is a self-pollinated crop having about 9 percent maximum natural out crossing. The plants are normally fully fertile, a few semi-sterile ones have also been reported.

Isolation distance :Cluster bean is a self-pollinated crop. Isolation distance of 50 metres for foundation and 25 metres for certified seed is essential.

Sowing :Cluster bean can be sown twice in a year, February - March in Northern plains and December - January in Southern plains.

Seed rate : In order to sow one hectare area about 30-40 kg seed of cluster bean is required.

Nutrition : The yield of cluster bean has been maximum when the crop was applied with 40 kg nitrogen and 60 kg phosphorus per hectare. The application of micronutrient to cluster bean crop proves very beneficial. Two sprays of molybdenum at 0.15 percent at 15 and 30 days after seedlings emergence give better yield.

Botany: The Cocoa (Theobroma cocoa) belonging to the family Sterculiaceae. Cocoa tree is a shade tolerant, moisture loving, understory rainforest tree. The trees live for up to 100 years, but cultivated trees are considered economically productive for only about 60 years. Naturally Cocao grows to a height of 15 meters, but cultivated trees are trimmed shorter to make harvesting easier. The leaves of Cocoa are smooth bright green, oblong, about 15cm by 8cm. The seeds are encased in a large colourful pod which grows close to tree after a flower. The large pod is green while maturing and turns yellow, orange, red or purple when ripe. They range from about 10 cm to greater than 40 cm in length.

Soil and climate: Potash rich alluvial soils friable in nature with high humus and moisture retentivity with a pH of 6.6-7.0 are suitable. Cocoa is normally cultivated at altitudes upto 1200 m above MSL with an annual rainfall of 150 cm and a relative humidity of 80 % and annual mean temperature of 24^oC to 25^oC. Cocoa can be grown as intercrop in coconut and arecanut gardens.

Botany: Coconut palm (Cocos nucifera) belonging to the family Palmae is a slender palm tree widely distributed throughout the tropical regions,with a size that can vary between 12 to 30 meters high. Each inflorescence is a branched flower cluster (panicle), located at the base of the leaves. Its flowers, male and female (monoecious), can be white to pale yellow. Fruiting occurs throughout the year, and the fruit is a drupe with a dry fibrous mesocarp popularly known as coconut.

Coconut fruit has an ovoid shape, constituted by 3 angles and 3 germination holes at the end, with a size between 20 and 30 centimeters in diameter. Coconut fruit consists of a brown fibrous bark and a hard shell, booking a seed inside. The central cavity of the fruit has an aqueous or milky liquid called coconut water that is a nutritional mixture aimed for coconut to grow. When the fruit ripens , this fluid accumulates fat and it is called coconut juice or milk. The seed of the coconut palm is the second largest known , after the coco-de-mer (Lodoicea maldivica ), that can reach 40–50 cm in diameter and weigh from 15 to 30 kg. The seed of coconut is hollow inside and it is covered with a white pulp, oily and edible.

Medicinal uses:It is said to have analgesic, antirheumatic, antibacterial , anticancer, antioxidant, hypoglycemic, hypotensive and immune stimulating properties. Its oil also has numerous cosmetic applications

Botany: Coffee trees are evergreen shrubs of the rubiaceae family. Coffee of commerce consists of seeds of coffee which when roasted, ground and soaked in hot water yields a fragrant stimulating infusion used for preparing the beverage called Coffee. It is native of America and introduced in India in 1936 near Chickmanglur in Karnataka.

Coffee plants have bright green opposite leaves with smooth margins. Its flowers, white, grow in clusters in the axils of the leaves and are aromatic. From them, fruits are born.They are red drupes with the size of a cherry The outside of the fruit is fleshy and inside it there are two seeds or coffee beans, surrounded by a membranous layer of leathery texture, hence this layer is commonly known as the “parchment”. Some species of trees only produce one seed per fruit are known as “pearl coffee”. In double seed varieties, the two seeds that grow inside each fruit press each other, so they stop the growth in the part which is in contact, so this becomes flat.

Soil and Climate: Coffee arbica comes up well in high altitude from 800 to 1650 meters but -C. canephora Pierre ex A.Froehner adopts well to lower elevations. At high altitude the crop is often late and susceptiable to frost and high winds.Evenly distributed rainfall of 2250 mm is essential. Heavy rainfall is not counducive as it encourages rotting of leaves and plants are easily susceptible to fungal diseases.

I. FILL UPS

1. The botanical name of radish is _____.
2. The botanical name of knol-khol is _____.
3. The botanical name of cauliflower is _____.
4. The botanical name of cabbage is _____.
   1. The botanical name of carrot is _____.

Cole crops refers to vegetable crops originated from a common ancestor, colewort or wild cliff cabbage (B. oleraceae var oleraceae or Brassica sylvestris L.) and they belong to family Brassicaceae. They prefer low temperature for growth and development hence mainly grown as temperate vegetables in temperate and subtropical climatic zones. This group of vegetable includes cabbage, cauliflower, knol khol, brussels sprout, sprouting broccoli, kale, Chinese cabbage etc. They are Mediterranean vegetables since its centre of origin is considered to be Mediterranean region. Later on, it spread into different parts of the temperate and subtropical countries. In India, cultivation of cole crops was started in the hills where temperate climate prevails and in states like Uttar Pradesh, Orissa, Bihar, Assam, West Bengal, Maharashtra and Karnataka as cool season crop. But with the introduction of tropical types which are more tolerant to high temperature cultivation, cole crops are taking new pace in the cropping system of nontraditional areas. Cabbage and cauliflower became the most widely grown cole crops though cabbage, knol khol and curly kale were the first cultivated types and cauliflower came in the mid-way followed by brussels sprout and sprouting broccoli. The genetic designation of different vegetables under cole crops were described by several workers of which Linnaeus (1753) designated all the cole crops under one species, Brassica oleraceae and in 1969, Neuwhof classified them into different forms as B oleracea var capitata L. (cabbage); B oleracea var botrytis L. (cauliflower); B oleracea var gemmifera DC. (Brussels sprout); B oleracea var italica L. (sprouting broccoli); B oleracea var gongyloides L. (knol khol) and B oleracea var acephala DC. (kale).

Cole crops are usually biennial herbs with tap root system and erect stem. They are highly cross-pollinated and temperature sensitive for the production of head and curd. They are classified as early, mid and late varieties based on the temperature requirement and duration. Among this, early varieties are found to be high temperature tolerant and late varieties are low temperature tolerant. The flowering in cole crops requires a chilling temperature of 4-70C for about 6 8 weeks which transforms the juvenile phase into reproductive stage with exceptions of some early varieties of cauliflower and sprouting broccoli. Upon receiving the chilling temperature, the central stalk will develop into reproductive shoot or multiple reproductive shoots will be formed from the curd. The earlier is seen in cabbage, brussels sprout and kale whereas, the latter in cauliflower and sprouting broccoli. The seed production of cole crops belonging to temperate groups are mostly undertaken in temperate hills especially in the hills of Uttaranchal and Himachal Pradesh whereas, seeds of tropical types are produced in plains and lower hills of India.

Varieties: No named variety

Soil: Red sandy soil and sandy loam soil are highly suitable. Soil drainage is essential and hard pan and water logged soils should be avoided.

Climate: Suitable for plain and lower hills. Requires 70 cm annual rain fall

Propagation: Terminal three to four nodal cuttings measuring 10 cm length is used

Land Preparation: Apply 15 t FYM/ha. Ridges are formed at a spacing of 60 cm. Cuttings have to be planted at a spacing of 45 cm.

Nutrient management: Nutrients Quantity (kg / ha) N 30 P 60 K 50 The above nutrients can be applied in two split doses viz., 30 and 45 days after planting. In micronutrient deficient soils, ZnSO4 can be applied as basal fertilizer.

PLANT EXPLORATION AND GERMPLASM COLLECTION OF PLANT GENETIC RESOURCES

Plant exploration and collection activities have been undertaken for a wide range of needs - by naturalists, travelers and plant hunters over the past several centuries in the quest for new and better economic plants in the southeast Asian region, ornamentals in the Himalayas and many others; by taxonomists for working out plant diversity and delineating phyto- geographic differences, and providing a basis for pinpointing areas with high species diversity and changes over time; world-wide collection, augmentation and characterization of crop plant diversity by Vavilov and co-workers (1926) for understanding the patterns of crop plant diversity and delineating centres of diversity; search for wild forms of species, and wild relatives of crop plants for working out primary centres of origin and crop genepools; biosystematic study, survey and in situ study and collection in protected areas for determining patterns of diversity build-up in crop and wild species. Concept of centres of origin of crop plants, importance of the wild genepool of crops, dependence on wild plants as a source of edible and otherwise useful species ensuing from these efforts provide the basis for systematic exploration and collection of plant genetic resources.

A

Abnormal seedling: Abnormal seedlings are those seedlings which do not show the capacity for continued development in to normal plants when grown in good quality soil under favourable conditions of water supply, temperature and light.

Abortion: Loss of reproductive structures (e.g. a flower or an ovule) during development or arrest of development of any organ so that it remains imperfect or fails to reach maturity.

Absolute humidity: The amount of water vapour present in a given volume of atmosphere, usually expressed in g water per kg dry air, or g water per cubic meter air.

Plant genetic resources represent both the basis for agricultural development and a reservoir of genetic adaptability that acts as a buffer against environmental changes. The erosion of these resources threatens world food security. From an agricultural utilitarian standpoint, plant genetic resources can be considered limited and perishable natural resources. PGR forms a distinct group and include all plant species, which contribute to people livelihoods by providing food, medicine, feed for domestic animals, fibres, clothing, shelter, energy and other uses and are subject to cultivation by man as well as the wild relatives of these species. Genetic diversity simply means all the variety of genes that exist in a particular variety or species. They provide the raw material (genes) which, when used and combined in the right way, produce new and better plant varieties, and are an irreplaceable source of such characteristics as resistance to disease, local adaptation, and productivity. Plant genetic resources are now, and will continue to be in the future, of inestimable value independently of whether scientists use them by means of conventional plant breeding or modern genetic engineering. These genes are dispersed throughout local cultivars and natural plant populations that have been selected over thousands of years by farmers and nature for their characteristics of adaptation, resistance and/or productivity. The road to a continuous increase in the production and quality of food lies through the protection and efficient utilization of plant genetic resources; this requires their conservation, evaluation, documentation and exchange.

Introduction
Understanding the concepts of technology transfer, licensing, and commercialization is crucial as it plays an important role in understanding how scientific discoveries leads to generation of new technologies further making them the impactful solutions that benefit industries and society. In the context of Indian HEIs, this especially important because a large volume of impactful research often remains underutilized due to limited commercialization channels. This chapter explains the terminology, essential components of technology transfer, outlines its significance for the innovation ecosystem. It also outlines the evolution of India’s technology transfer ecosystem, and emphasizes while understanding its potential to boost India’s economy, encourage entrepreneurship, and strengthen global competitiveness. From initial idea to a market-ready product is complex and multiphased process. The stepwise process is shown in Figure 1. It highlights a structured pathway consisting of five core phases: Idea/Concept, Technology development, Intellectual Property (IP) rights, Technology transfer, and Technology commercialisation.
Phase 1: Idea/Concept stage
This process of technology transfer starts with the Idea/Concept phase, where an invention is created. Each phase plays an important role in transforming raw scientific knowledge into innovative solutions that benefit society and generate economic value. In this phase breakthrough ideas emerge, within labs. Researchers explore and develop initial proof-of-concept or prototypes to validate their ideas. At this stage, the focus is primarily on knowledge creation rather than commercialization. This pathway of technology transition is summarized in Figure 1.

The basic feature of the Pre-British India was a village, which was economically a water-tight compartment fulfilling the three basic functions of economy, i.e., production, distribution and consumption, within the village community without outside world dependence, except the salt and kerosene. Every village was a self-sufficient unit with dependence on agriculture carried out by community people of different castes having the occupational specialization with interdependence, viz. jajmani system and alike.

Developments in the means of communications and the opening of Suez Canal in 1869 introduced fundamental and far-reaching changes in the social system and economic life of Indians by transforming self-sufficient village economy to new modes and means based on competition, market and price mechanism. This change was deliberated by Britishers for meeting the requirement of raw material and the diversification to tea, jute and indigo took place. Cotton also got importance but agriculture did not find place for vegetables. Famines were very common and crop production depended on monsoon rains to the large extent. Britishers developed cotton, jute and mineral trade for their vested interests.

Britishers emphasized the development of railways for better mobility of their troops, commercial crop produce and minerals but the Report of Famine Commission 1901 bitterly criticized the Government for the step-motherly treatment given to irrigation facilities as compared to railways. The irrigation policy of the Government in 19th century was determined purely from concerns for adequate returns on investment and profit was of Rs. 81 lakhs after paying 4% interest on amount invested on irrigation development in 1896-97. Irrigation Commission was established and a sum of Rs. 42 crore was spent on irrigation by 1911. By 1930, about 30 million acres were brought under canal irrigation, which was far larger than the irrigated area in any other country of the world. Even in the USA, the area under canal irrigation was two-third that of India. Vegetables cultivation did not find place in Indian agriculture and also in diet of villagers. Their cultivation remained restricted to the periphery of the urban centres for meeting the demand of upper class elites only.

Botany:Coriandrum sativum belonging to the family Umbelliferae. It is an annual soft shrub that attains a height of 1 to 3 feet. Leaves are in basal cluster varies from 3 to 15 cm in length and are oblong to ovate in shape. Leaflets are 1 to 2 centimeter in length and are ovate to round. Flower is of white color which can vary from light purple to creamish in color. These appear in umbles. Fruit is roundish in shape bearing color that varies from yellow to brown. It is tripped and gets divided in to two then crushed with fingers. The plant flowers and fruits in late winters.

Land Requirement: Land to be used for seed production of coriander should be free from volunteer plants

Varieties: CO 1, CO 2, CO 3, CO (CR) 4, GAU 1, UD 1, UD 2, UD 20 and UD 21

Soil: Well drained black cotton soil and red loamy soil. For rainfed cultivation soil should be clay in nature and the pH should be 6 – 8.Coriander performs well at a temperature range of 20 – 25 °C.

Climate: Cool and comparatively dry, frost free climate

I. FILL UPS

1. _____ is called as ‘king of fibres’ and ‘white gold’.
2. _____in the cotton meal is toxic to monogastric animals.
3. A purple spot is found in the claw of the petals is called _____
4. The isolation distance adopted for cotton is _____ for foundation seed.

Botanical description

Cowpea belong to genus Vigna and species unguiculata (L) Walp (Syn V. sinensis Savi) of the family Leguminosae sub family Fabacae.

Floral biology

The flowers of cowpea are hermaphrodite. Self-pollination is effected though with some cross-pollination.

Climate and Soil

Cowpea is a warm season crop, and therefore it can be grown both in spring and in rainy seasons in the plains of India. It cannot tolerate cold weather, heavy rainfall and water logging.

I. FILL UPS

1. Cowpea is a _____ crop.
2. Isolation distance for cowpea foundation seed production is _____.
3. Spacing adopted for cowpea is _____.
4. Seed rate for cowpea seed production is _____.
5. The maximum permitted limit of offtypes for CS is _____.

Cucurbits account for the largest group of tropical vegetables, consisting of cucumber, muskmelon, bitter gourd, bottle gourd, ridge gourd, sponge gourd, snake gourd, pointed gourd, bitter gourd, ivy gourd, wax or ash gourd, chow chow or chayote, pumpkin, summer squash, winter squash, watermelon etc. They all belongs to the family Cucurbitaceae. They are herbaceous in nature and are characterized by tendril-bearing vines and adaptation to warm climate of the tropics and subtropics. Among the different genera of cucurbit vegetables, three genera viz., Cucumis (cucumbers, melons), Cucurbita (pumpkins, squash), and Citrullus (watermelons), are economically important in the world while the others hold regional importance. Most of the cucurbits are grown for its immature fruits and are consumed as salads or cooked vegetable. While, muskmelon and watermelon are used fresh as dessert fruits. Pumpkin and squashes are used in confectionaries besides as cooked vegetables. Mature ash gourd fruits are used in sweet candy preparation.

Cucurbits are low in calories, minerals, or vitamins. However, bitter gourd is rich source of vitamin C, the squash, pointed gourd, and muskmelon contain good amounts of carotene, kakrol is high in protein, and pointed gourd is rich in calcium. Bitterness in some species of cucurbits was attributed by the varying degree of bitter principles, cucurbitacin. Bitter gourd is known for its hypoglycemic activity due to phytoceutical, cheratin in the fruit, which lowers blood sugar level.

Storage of paddy seed lots after harvesting is one of the crucial aspects of processing shopfloor. Paddy due to increased mechanised harvesting may loose vital seed quality parameters. Since seed growers cannot buy a combined harvester themselves-they resort to hiring of this machine. Sometimes in the hurry of completing harvesting they harvest the crop at un-safe moisture content. This un-safe moisture content may go up to 50-60% also. Harvesting of seed crop with high moisture has adverse effect on storability. Ensuring safe seed moisture is very critical especially during rabi-summer seed production cycle. Most of the growers depend on sun drying for reducing the seed moisture. But paddy should not be dried >3% in one cycle. Follow tempering and convection cooling after every cycle and repeat the cycle till seed attains safe moisture content (14%).

Objective

    1.    Design drum priming (2-3 kg and drying unit with house hold or commonly available materials.
    2.    Standardization of time for seed priming for different crops.

Probable drum priming unit components

A] A vertical drum which need to be rotating
B] A motor which will rotate
C] A desktop assembly
D] A simple two way seed drier (Fig not given here)

I. FILL UPS

1. _____ is the most important source of varietal deterioration during seed production.
2. _____ in oats is caused due to mutation.
3. In peas, _____ is caused due to mutation.
4. _____ is another most important source of varietal deterioration due to introgression to genes from unrelated stocks.
5. _____ is the major source of genetic contamination and varietal deterioration in cross fertilized crop.

Genetic purity (trueness to type) of a variety can deteriorate due to several factors during production cycles. The best means to insure genetic purity therefore, would be to overcome to the greatest possible extent the various factors responsible for genetic deterioration. The important factors of apparent and real deterioration of varieties as listed by Kadam (1942) are as follows:

Developmental variations

Mechanical mixtures

Mutations

Natural crossing

Minor genetic variations

Selective influence of diseases

The technique of the plant breeder

Mechanical damage is inflicted on seeds during harvesting, threshing drying, processing and handling. Injury may occur from any kind of physical abuse. Threshing abuse probably is most serious but, seed conditioning and handling also contribute to seed injury. High temperatures during drying or drying too quick or excessively can cause injury. Susceptibility to mechanical injury increases as the moisture content of the seed decreases. Effect of all individual impacts received by the seed appears to be cumulative in total seed damage. Cereals are largely immune to mechanical injury presumably because of the protective outer structures, the palea and lemma. Large sized legumes are more suscepitable. Small seeds tend to escape injury during harvest and seeds that are spherical tend to suffer less damage than elongated or irregularly shaped ones.

1. VARIETY

Variety is a seed/crop which suit the prevailing agroclimatic conditions, high yielding and possessing desirable attributes such as disease resistance, earliness and grain quality. Similarly, the seed should be pure, appropriate classes, and obtained from an authorised official agency.

I. FILL UPS

1. _____ is a crossing between two genetically dissimilar parents.
2. Detasseling is done in _____.
3. _____ is an example for chemical hybridizing agent.
4. _____ is called as pollen parent.
5. _____ is the fertile counter part of A line.
6. DUS stand for _____.

Dietary constitution of Indian people is mostly cereal based. However, the cereals supply chiefly carbohydrates. Therefore, there is deficiency of protein, minerals and vitamins, especially vitamin A, riboflavin, vitamin C, etc. in the diet. The dieticians advocate the intake of 125 g leafy vegetables, 75 g other vegetables and 100 g root and tuber vegetables (total 300 g) everyday because vegetables provide all the nutrient components like carbohydrates, protein, fat, minerals, vitamins and water along with roughages, which are the essential constituents of a balanced diet.

India has attained food security by increased cereal production due to green revolution. In case of vegetables, although there has been increase in production from 15 million tonnes during 1950 to 94 million tonnes in 2005 but still more vegetables are needed to meet the minimum requirement of at least 250 g per day per capita. There has also been increasing demand for fresh export and for processing industries. Moreover, there is a pressure for increasing vegetable supply caused by constantly increasing population in a world of limited acres.

Addition of new agricultural land is of course not possible. In this situation, plant breeding can be expected to contribute substantially to greater vegetable productivity. This can be accomplished not only by the breeding of basically higher yielding varieties but also by the development of varieties that help to stabilize production through resistance to various biotic and abiotic stresses.

Genetic diversity is one of the fundamental character present in any population or species. It is the total variation either phenotypic or genotypic which have occured in a population through due course of evolution. Variation in any species depends on mutation, substitution (insertion/deletion) and response to selection. However, comprehensive studies on theoretical and empirical perspectives for diversity analysis may be found by evolutionary biologists to understand the factor that influences levels of genetic diversity in natural population (Lewontin, 1974). In brief, nucleotides/ protein codes are the basic unit to quantify total amount of variation. Bioinformatic tools and techniques help us to measure inferring relationships between genes, proteins or organisms through use of these DNA/protein sequences by integration of mathematical algorithms, statistical and computer databases.

Diversity analysis is popularly known as phylogenetic analysis in bioinformatic terms. The basic principle of phylogeny is analysis/alignment of characters; whether it is morphological or molecular data. For instance, in case of morphological data of a crop species, the leaf colour/stem colour/fruit colour/grain size etc. may be compared however sequence data is problem of interest for molecular phylogeny. The evolutionary history of any crop species/ organisms inferred from phylogeny analysis mostly depicted through treelike diagrams/ dendrograms. In this chapter, phylogenetic analysis based on only nucleotide/protein sequences are discussed. For analysis of phylogenetics, default assumptions (Baxevanis and Ouellette, 2006) are:

Previous Year Questions

1. What is DNA fingerprinting? Discuss the applied aspects in modern agriculture. (15M, CSE 2021)

• The term DNA fingerprinting in its original sense refers to the method developed in 1985 by Sir Alec Jeffreys (Jeffreys et al., 1985) and his associates for the simultaneous detection of highly variable DNA fragments by hybridisation of specific multilocus probes to electrophoretically separated restriction fragments?

• The DNA fingerprints, resembling barcodes, are unique to the individual and hence can be used in much the same way as conventional fingerprints - to identify individuals with absolute certainty?

DNA Fingerprinting Techniques

• A large number of fingerprinting techniques are in currency, which are variations and combinations of restriction fragmentation and polymerase chain reaction (PCR).

• Restriction fragment length polymorphism (RFLP) involves digesting DNA with restriction enzymes, separating the resultant DNA fragments by gel electrophoresis, blotting the fragments to a filter and hybridising probes to the separated fragments. Specific probe-enzyme combinations give highly reproducible patterns.

• With the advent of PCR technology a number of simpler techniques have become available for molecular characterisation.

• These include, random amplified polymorphic DNA (RAPD) and its several variants, intersimple sequence repeats (ISSR), amplified fragment length polymorphism (AFLP) and sequence tagged microsatellites sites (STMS).

1. DUS TEST

A variety shall express distinct character(s) for verification, uniformity within population and stability for expression of such characters over the year and at different locations, then only its genetic purity is confirmed.

1.1. Requirements

Authentic seeds of existing varieties, pure seeds of variety under tests, list of characters to be examined and their expression.

This test is conducted in the most optimum conditions of soil and climate and the crop is exposed to the best agronomic management conditions to ensure full expression of the characters.

1.2. Distinctness

The variety under test is compared with the existing varieties (having similar plant height, growth habit, days to flowering, days to maturity and reaction to diseases) for qualitative morphological characters at seed, seedling and plant levels. Biochemical differences may also be considered. To establish the distinctness, the test is performed for 2 years for self pollinated crops.

Introduction

In order to implement the sui generis system for plant variety protection for granting PBR to a breeder or farmer or institution, DUS testing is compulsory. A new variety shall be registered, if it conforms to the criteria of novelty, distinctness, uniformity and stability. An extant variety, a variety about which there is a common knowledge shall also be registered within a specified period, if it conform such criteria as distinctness, uniformity and stability.

Once a valid application for PBR is accepted the seed / plant material of the variety will be requested for official DUS tests. These official DUS tests need to be conducted by crop based institutions on behalf of the PPV & FR authority by following universally acceptable test procedures. This necessitated in the development of test guidelines, descriptors and procedures to be followed in each crop. National Test Guidelines have been developed for 96 crops representing harmonized approach for the testing of new varieties which will form the basis for DUS examination. These National Test Guidelines contain details on i) subject of the guidelines, ii) material required, iii) conduct of tests, iv) methods and observations, v) grouping of varieties, vi) characteristics and symbols, vii) table of characteristics, viii) explanations on table of characteristics, ix) literature and x) technical questionnaire. The registration was open for 107 crop species for new variety and for 114 crop species for extant / farmers varieties.

Onion is a major global commodity, but its value chain is characterized by price volatility, post-harvest losses, fragmented marketing and wide differences between farm-gate and retail prices. This chapter analyses production, trade, marketing and supply-chain trends from global and Indian perspectives. It examines India’s role as a leading producer and exporter and discusses the APMC system, intermediaries, storage deficiencies, Farmer Producer Organisations and export-policy interventions. Digital platforms and technologies—including e-NAM, blockchain traceability, IoT-enabled storage and artificial-intelligence-based price forecasting—are considered for improving transparency, efficiency and coordination. Strategic interventions are proposed to create a more resilient, inclusive, competitive and technology-enabled onion economy

Seed is the primary and essential starting point of a wide range of crops, including the majority of vegetables except those vegetative propagated (e.g- potato and sweet potato). Seedling emergence and field stand establishment is one of the problems facing the growers, especially in early planting where adverse conditions are prevailed (low temperature and high soil moisture). Seed germination and seedling emergence are affected by many factors such as: seed genotype, seed quality (seed viability and seed vigor) and the environmental conditions (moisture and temperature) prevailing during seed germination and subsequent seedling emergence, assuming that the seed is not dormant.

This practical in continuation of the practical no.10 explore the effects of screen sizes on different varieties. The screen sizes vary not with crops but with varieties also. To know the effect of screen sizes among the varieties we need to take the top and middle screens as standard or as recommended. These methods can be applied to any crop and to different varieties.

Elephant foot yam is an underground stem tuber belonging to the family Araceae, one of the most popular tuber crops, extensively used as a favourite vegetable by millions of people in India. It has both nutritional and medicinal value and is usually consumed as cooked vegetable. It has high dry matter production capability per unit area than most of the other vegetables. Elephant foot yam is a remunerative and profitable stem tuber crop. The crop is gaining popularity due to its shade tolerance, easiness in cultivation, high productivity, less incidence of pests and diseases, steady demand and reasonably good price.

I. FILL UPS

1. The developing ovule is commonly attached to the placenta by the _____.
2. The scar on the ovule made where the funiculus detaches at maturity is known as the _____.
3. The point where the integuments meet at the nuclear apex is _____.
4. Opposite to the micropyle, the region of integumentary origin and attachment is called _____.
5. Between the chalaza and the hilum is an area called _____.
6. _____ is an example for species with developed perisperm.

Conventional fertilizers often exhibit low nutrient-use efficiency, resulting in nutrient losses, soil degradation, water contamination and unnecessary production costs. Nano-fertilizers offer controlled and targeted nutrient delivery through formulations engineered at the nanoscale. Their high surface-area-to-volume ratio can improve nutrient availability, plant uptake, growth, bulb quality and storage performance while reducing conventional fertilizer requirements. This chapter explains the nature, modes of action and potential benefits of nano-fertilizers in onion production. It also considers nano-biofertilizers, smart nutrient-delivery systems and nano-sensors. Particular attention is given to manufacturing cost, nanotoxicity, effects on soil microorganisms, farmer accessibility and the need for standardized safety and regulatory frameworks.

Abstract

Forestry in India is very important for its wood, fuel and many non-wood products which were being used from ancient times by our forefathers for different personal and commercial use. Farmers face lots of problem in forestry because of its long gestation period, dependence on nature, bulkiness of outputs, season oriented production, perishable in nature, lack of processing centres for value addition at farm and intermediate levels, price fluctuation of forestry products, forced sale/distress sale of tree growers, low wage rate to workers in forestry activities and farmers in sufficient knowledge on market information. Establishment of institutional setup to promote forestry is urgent in the recent times.

Onion growth, bulb initiation, yield, quality and storage life are strongly influenced by temperature, photoperiod, soil, moisture and crop establishment. Different cultivars require specific day lengths and temperature ranges, making regional variety selection and planting schedules essential. This chapter explains the environmental requirements of onion and the consequences of unsuitable conditions, including premature bolting, double bulbs, poor development and disease susceptibility. It examines soil preparation, planting geometry, direct seeding, transplanting and precision establishment. Modern approaches—including sensor-based environmental monitoring, Internet of Things systems, predictive modelling, precision seeding and machine-learning-supported input management—are presented as tools for improving uniformity, resource efficiency and climate adaptability.

The establishment of a technology transfer office is an important and complex process for an organization. It needs careful planning to ensure its long-term sustainability. Before establishing a TTO, it is essential to evaluate whether institution’s core mission includes research commercialization, and if the volume and quality of its research output justify the need for an inhouse TTO. For institutions that may not meet all criteria, those institutions can collaborate or outsource for TTO services. It is also important to decide about its operational scope, funding, management, and for what kind of issues the office should develop a policy. There is no “right” way to set up a TTO, but successful TTO does require considering some key issues. This chapter outlines key steps and considerations for establishing a TTO. It also explains what physical infrastructures are needed, manpower, outlines the responsibilities and powers of TTOs.
2.1 Evaluating the need for a TTO
Establishing a TTO is an important step for any research focused organization. But before initiating a planning process for a new TTO, the organization must review its relevance by considering a few fundamental points:
Key considerations for institutions
1. Is institution’s mission focused on “research commercialization” If an institute’s primary mission is education, or it does not support research it might not be justified to create a TTO. For such institutions, it usually makes better sense to seek alternative solutions to the occasional technology transfer without the expense of a formal office. But institutions with strong research commercialization focus, a TTO has the ability to contribute meaningfully to the mission. The Association of University Technology Managers (AUTM), USA has identified following key motivations for initiating academic technology transfer:

1. SEED TESTING

The science of evaluating the planting value of seed has been developed to achieve the following objectives for minimizing the risks of planting low quality seeds.

To determine the seed quality and its suitability for planting

To identify seed quality problems and their probable cause.

To determine the need for drying and processing, if any.

To determine if seed meets established quality standards for labeling.

To establish quality and provide a basis for price and consumer discrimination among lots in the market.

1. INTRODUCTION

Field run seeds obtained from the seed production field posses high moisture and physical contaminants like trash and other inert materials (sand, stones, soil etc.,), diseased and pest damaged seeds, other crop seeds, weed seeds, deteriorated and damaged seeds, off-size and ill filled seeds, plant remnants etc. Seed processing is must to dry the seeds to safe moisture level, remove unwanted and inert materials as detailed above.

Seed qualities such as vigour and viability are improved by certain physical characteristics of the seed i.e., shape, size, large, denser and sound seed, etc. So, if grading is done to obtain a particular range of size, shape, length and density of the seeds, the quality of the lot is upgraded.

Seed processing refers to all the steps necessary for preparation of harvested seed for marketing namely handling, drying, shelling, preconditioning, cleaning, size grading, treating and packaging.

The physical properties of seeds are a basic activity for designing processing equipments and precision planters. Since these characters vary with crop or variety, one needs to have the data handy to be used on shop floor. This data also helps to quantify processing efficiency, presence of dormancy and suitability for seed treatments for all existing and new crops or varieties.

There are stages in seed supply chain where seed losses happen at harvesting and post harvest stage. These losses may occur due to higher moisture at harvest, due to harvesting methods, processing methods, at seed storage, during transport etc. Estimation of these losses is an important function of seed inventory management. Estimating seed losses help to improve processing and seed distribution efficiency. These simple statistical tools will help to measure quantitative post harvest seed losses. Seed testing can be used for measuring qualitative post harvest seed losses.

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

Exotic vegetables are those vegetables that are introduced or not common in India. Its area under cultivation is comparatively less and most of the growing area surrounds the targeted markets especially five star hotels and restaurants. Now a days, there is an increasing trend in cultivation of these high value vegetables because of increased demand and good profit. Farmers could sell these vegetables at a at a higher price when compared to traditional vegetables. They are mostly cultivated in net houses or poly houses. Some of the exotic vegetables popular in India are asparagus, celery, broccoli, lettuce, parsley etc.

Asparagus (Asparagus officinalis)

Asparagus is a dioecious monocot which produces tender succulent above ground stems known as spears from fleshy underground crown. These tender spears are used as vegetable, for cooking purpose and for the preparation of soups. The white crystalline substance, asparagine present in the juice of young shoots have diuretic properties and is used in cardiac dropsy and chronic gut. Asparagus is mainly cultivating in countries like USA, Germany, Spain, France etc. while in India, it is cultivating only in a negligible area. Asparagus is grouped under cool season vegetables. But newly emerging spears are susceptible to frost though a wide range of environmental conditions can be tolerated by mature asparagus. Asparagus is native to temperate Europe and Asia and has been cultivating for 200 years.

Morphology

Asparagus is perennial in nature and produce erect branched stem and grows upto a height of 1 to 3 m. The crop yield continuously for 10-15 years from the initial year of spear production. Asparagus roots are tuberous and the leaves are narrow and needle like. Male and female flowers are produced in different plants.