eChapters

Introduction
Understanding the concepts of technology transfer, licensing, and commercialization is crucial as it plays an important role in understanding how scientific discoveries leads to generation of new technologies further making them the impactful solutions that benefit industries and society. In the context of Indian HEIs, this especially important because a large volume of impactful research often remains underutilized due to limited commercialization channels. This chapter explains the terminology, essential components of technology transfer, outlines its significance for the innovation ecosystem. It also outlines the evolution of India’s technology transfer ecosystem, and emphasizes while understanding its potential to boost India’s economy, encourage entrepreneurship, and strengthen global competitiveness. From initial idea to a market-ready product is complex and multiphased process. The stepwise process is shown in Figure 1. It highlights a structured pathway consisting of five core phases: Idea/Concept, Technology development, Intellectual Property (IP) rights, Technology transfer, and Technology commercialisation.
Phase 1: Idea/Concept stage
This process of technology transfer starts with the Idea/Concept phase, where an invention is created. Each phase plays an important role in transforming raw scientific knowledge into innovative solutions that benefit society and generate economic value. In this phase breakthrough ideas emerge, within labs. Researchers explore and develop initial proof-of-concept or prototypes to validate their ideas. At this stage, the focus is primarily on knowledge creation rather than commercialization. This pathway of technology transition is summarized in Figure 1.

Need for certification

Forest restoration and reforestation experts know the effect the right seed source can have on the long-term economic and ecological success of tree planting efforts. However, unlike stock quality (such as size and health), genetic quality (seed source) cannot be observed by the consumer at the time of stock purchase. Where once the provincial government controlled the source of seed and its destination through the provincial nursery system, seed supply is now in the private sector, and the source is unknown or a matter of trust. Tree planting in general needs help, and the wrong seed source is only one of the many reasons that your planting project may not be successful. Besides, the identity of the source will be tracked throughout the chain of custody until it is presented, as a labeled, certified product to the consumer. The seed source may be from a single parent tree or preferably from a large healthy stand of trees. But if seed is bulked from many locations, the label on the resulting seedlings can only indicate the greater area. For example if a nursery bulked its seed from many counties across a specific place, they can only certify and market that crop as? specific place? not as one from a specific county that may have been a source for the seed. For this reason we will have to encourage collectors and growers to bulk only by tree seed zones to promote certification program for the context of successful tree planting.

Seed testing

No matter how good processed seed may look, the actual value is the number of seedlings it will produce. Testing of seeds is therefore imperative to determine and identify the changes that have occurred from the time of collection until the seeds are sold or sown at one time. The critical step in evaluating a seed lot is the drawing of representative sample. Place all the test samples in a bucket, mix them thoroughly and subdivide to obtain sample size. Improvements in seed processing can only be obtained with quality control. These evaluations should be made in the processing plant and at a seed testing laboratory. The National Tree Seed Laboratories offers a free service to evaluate processing machinery. You need only collect a small sample of seed after it has been processed by each machine and send it to the laboratory. Be sure all the seed comes from the same lot so all samples are related. Each machine must be adjusted for efficient operation, and proper adjustment requires monitoring of the operation.

Seed dormancy

Plants evolve many mechanisms that ensure their survival. For instance, some species produce prodigious number of seeds so that even if only a small proportion germinate and grow, some seedlings will survive. Alternately, dormancy can be defined as: a seed characteristic, the degree of which defines what conditions should be met to make the seed germinate. This definition may be more useful in understanding dormancy because it suggests that dormancy is variable, rather than constant, which is the situation that we generally encounter in the native populations of many species. The staggering of germination (dormancy) is an advantage for seeds that mature in late summer to early fall, since immediate germination would leave vulnerable seedlings exposed to harsh winter conditions.

The decision whether to extract the seed from the fruit at a central seed processing depot or close to the site of, and soon after, collection must be made in the light of local conditions. The reduction in bulk and weight of the collected material greatly facilitates transport and for this reason the extraction of the seed from relatively bulky fruits may be desirable at the site close to the collection area. In some cases it has also been found that early extraction of the seed is essential to maintain maximum viability. The use of the sun drying to speed extraction of the seed is common for many conifers and trees with woody dehiscent fruits. However, too rapid drying of fruits may sometimes cause them to remain closed and prevent later extraction, a condition known as “case-hardening” of cones. This is true for the cones of some pines if they are collected before peak maturity. In such cases it is necessary to keep the fruits under cover, with a good circulation of air, for one or two weeks before more rapid drying is attempted.

The collection of cones/fruits and seeds is the first step in the harvest, processing, and storage of seed in which man interacts with nature in determining ultimate seed quality. Successful collection, therefore, depends on understanding seed maturation and dispersal characteristics of each species, knowing local weather trends, and evaluating crop quality. Collection of propagating material is an expensive process and it is therefore also important that areas wherein cones and seeds are to be collected are evaluated to determine whether a sufficiently heavy crop to permit economic collection of seeds is present and whether the seed zone and elevation represented by the candidate area is appropriate for the production of seedlings needed in the reforestation program. Remember that properly collected, processed, and stored seeds may remain viable for a decade, so evaluation of seed needs may be projected well in advance of current harvest schedules through proper planning and organization of seed collection.

Seed bearing plants

Seed-bearing plants are broadly categorized in two major types: the gymnosperms and the angiosperms which are together grouped in Division Spermatophyta in the older system of classification for the plant kingdom. Consequently, seed plants are presently classified into multiple phyla. Still, for the purpose of learning basic concepts, it is convenient to group plants informally as spermatophytes (sperm "seed" + phyte "plant"). The spermatophytes are the most complex and evolutionarily advanced plants. Unlike ferns and bryophytes which reproduce and disperse by unicellular spores, spermatophytes reproduce and disperse by means of multi-cellular seeds which consist a protective seed coat surrounding an embryonic sporophyte and its food supply. This group of seed plants exhibits an alternation of generations in which the sporophyte is dominant. Furthermore, the gametophyte is microscopic and heterosporous with the latter term meaning that these seed plants produce two types of spores: microspores and megaspores that give rise to separate male gametophytes (micro-gametophytes) and female gametophytes (mega-gametophytes) respectively. The megaspore is never released from the sporangium; thus, the female gametophyte develops within the sporangium while being still attached to the sporophyte.

Previous Year Questions

1. Intellectual property rights (IPR) are the rights given to persons over the creations of their minds: inventions, literary and artistic works, and symbols, names and images used in commerce. They usually give the creator an exclusive right over the use of his/her creation for a certain period of time?

2. Plant varietal protection?

• The importance of intellectual property was first recognized in the Paris Convention for the Protection of Industrial Property (1883) and the Berne Convention for the Protection of Literary and Artistic Works (1886). Both treaties are administered by the World Intellectual Property Organization (WIPO).

Previous year questions

1. Introduction?

2. Role of private? Introduction

• The Indian seed industry used to be dominated by public sector seed companies. However, following the easing of government regulations and the implementation of a new seed policy in 1988, the private sector seed companies have started playing a major role in seed development and marketing.

• More recently, the government’s decision to embrace biotechnology as a means of achieving food security has attracted several leading biotechnology-focused multinational seed companies to India.

• The composition of the seed industry, by volume of turnover, has reportedly reached a ratio of 60:40 between the private and public sectors.

Previous Year Questions

1. What is DNA fingerprinting? Discuss the applied aspects in modern agriculture. (15M, CSE 2021)

• The term DNA fingerprinting in its original sense refers to the method developed in 1985 by Sir Alec Jeffreys (Jeffreys et al., 1985) and his associates for the simultaneous detection of highly variable DNA fragments by hybridisation of specific multilocus probes to electrophoretically separated restriction fragments?

• The DNA fingerprints, resembling barcodes, are unique to the individual and hence can be used in much the same way as conventional fingerprints - to identify individuals with absolute certainty?

DNA Fingerprinting Techniques

• A large number of fingerprinting techniques are in currency, which are variations and combinations of restriction fragmentation and polymerase chain reaction (PCR).

• Restriction fragment length polymorphism (RFLP) involves digesting DNA with restriction enzymes, separating the resultant DNA fragments by gel electrophoresis, blotting the fragments to a filter and hybridising probes to the separated fragments. Specific probe-enzyme combinations give highly reproducible patterns.

• With the advent of PCR technology a number of simpler techniques have become available for molecular characterisation.

• These include, random amplified polymorphic DNA (RAPD) and its several variants, intersimple sequence repeats (ISSR), amplified fragment length polymorphism (AFLP) and sequence tagged microsatellites sites (STMS).

Previous Year Questions

1. Different Classes of Seeds?

2. Various parameters of field & seed standards for breeder seed production?

3. Factors that affect the longevity of seeds during storage in warehouses?

4. Initial Seed Quality?

5. Seed Testing?

Different Classes of Seeds

1. Nucleus Seed: It is handful of seed maintained by concerned breeder for further multiplication. The nucleus seed will have all the characters that the breeder has placed in it and it is of highest genetic purity. The quantity of nucleus seed is in kilograms.

2. Breeder Seed (BS): Breeder seed is a seed or vegetative propagating material directly controlled by the originating or sponsoring plant breeder of the breeding programme or institution and or seed whose production is personally supervised by a qualified plant breeder and which provides the source for the initial and recurring increase of foundation seed. Breeder seed is exempted from certification. However, the breeder seed crop is being inspected at appropriate stages by a Monitoring Team consisting of breeder, representative of NSC and representative of Seed Certification Agency. The breeder seed lots are affixed with Golden Yellow tags. The genetic purity of breeder seed crop should be maintained at 100 per cent.

Previous Year Questions

1. What is seed treatment?

2. Factors affecting the seed quality of a crop during seed production?

3. Seed treatment campaign – Role & importance?

4. Agronomic and genetic principles of quality seed production?

5. Varietal seed production and hybrid seed production?

Seed Treatment

Seed treatment refers to the application of fungicide, insecticide or both to the seeds to disinfect(deep seated) and disinfest (over seed coat) them from seed borne or soil borne pathogenic organisms and storage insects. It also refers to subjecting the seed to solar energy exposure or immersion in conditional water.

Spices are those plants, the products of which imparts strong flavour and aroma to the food. This is attributed to the presence of compounds such as volatile oils present in the plant parts. India is renowned for wide variety of spices and is designated as land of spices. Spices are indispensable ingredient in almost all Indian dishes and cuisines foreign invaders invaded India for spices in ancient times. India is figured as the largest producer, consumer and exporter of spices and spice products in the world. Turmeric and Ginger are the major rhizomatous spices of vegetable origin widely cultivating and using in India. They are known for its antimicrobial and antioxidant properties, hence using in medicines for the treatment of various ailments. Apart from these rhizomatous spices, India is also contributing a major share in the production of seed spices. Around twenty spices among sixty-three spices growing in our country are classified as seed spices. Major seed spices of India are coriander, cumin, fennel, fenugreek, dill, ajowain, celery, anise, nigella, caraway etc. These seed spices are extensively cultivated in the arid to semi-arid region of India as Rabi crop.

It is estimated that, spice production in our country increased from 67.64 lakh tonnes in 2014-15 to 106.79 lakh tonnes in 2020-21 with an annual growth rate 7.9%. There is a corresponding increase in the area of cultivation also. An increase in area from 32.24 lakh hectare to 45.28 lakh hectare was observed. Among the major spices, cumin (14.8%), garlic (14.7%), ginger (7.5%), fennel (6.8%), coriander (6.2%), fenugreek (5.8%), red chilli (4.21%) and turmeric (1.3 %), showed significant growth rate in production. The export of spices contributes around 41% of the total export earnings from all horticulture crops in the country and it ranks fourth among agricultural commodities, after marine products, non-basmati rice and basmati rice.

Exotic vegetables are those vegetables that are introduced or not common in India. Its area under cultivation is comparatively less and most of the growing area surrounds the targeted markets especially five star hotels and restaurants. Now a days, there is an increasing trend in cultivation of these high value vegetables because of increased demand and good profit. Farmers could sell these vegetables at a at a higher price when compared to traditional vegetables. They are mostly cultivated in net houses or poly houses. Some of the exotic vegetables popular in India are asparagus, celery, broccoli, lettuce, parsley etc.

Asparagus (Asparagus officinalis)

Asparagus is a dioecious monocot which produces tender succulent above ground stems known as spears from fleshy underground crown. These tender spears are used as vegetable, for cooking purpose and for the preparation of soups. The white crystalline substance, asparagine present in the juice of young shoots have diuretic properties and is used in cardiac dropsy and chronic gut. Asparagus is mainly cultivating in countries like USA, Germany, Spain, France etc. while in India, it is cultivating only in a negligible area. Asparagus is grouped under cool season vegetables. But newly emerging spears are susceptible to frost though a wide range of environmental conditions can be tolerated by mature asparagus. Asparagus is native to temperate Europe and Asia and has been cultivating for 200 years.

Morphology

Asparagus is perennial in nature and produce erect branched stem and grows upto a height of 1 to 3 m. The crop yield continuously for 10-15 years from the initial year of spear production. Asparagus roots are tuberous and the leaves are narrow and needle like. Male and female flowers are produced in different plants.

Leafy vegetables are rich source of vitamins like A, C and minerals like calcium and iron. Most of the leafy vegetables are quick growing types and shows good response to manures and fertilizers. Their easiness in cultivation and adaptation to diverse agroclimatic conditions makes it suitable for various cropping systems. Some of them are annual in nature while some others are perennial. Leaves as well as tender stems are uses as vegetable in the cooked form. They are reported to have antinutritional factors, oxalates and nitrates compared to other vegetables. The annual leafy vegetables are widely cultivating in our country and among them, amaranth, palak, spinach and fenugreek are the major ones. These crops are short duration in nature, completes their entire life cycle by 80-90 days and produce good yield and brings monetary returns to farmers in short time.

Amaranth (Amaranth sp.)

It is herbaceous fast growing annual leafy vegetable grown for leaves as well as grains. Vegetable amaranth is reported to be equal or superior in taste to that of spinach. Leaves and succulent stem are good source of vitamin A, vitamin C, calcium and iron and are used as vegetable. High oxalate and nitrate content are reported in some species of amaranth.

The major vegetable species of amaranth cultivating in India is A. ticolor. It is suggested to be originated in South or South east Asia particularly in India. Another vegetable type, A. dubius shows diversity in Central America, Indonesia, Asia and Africa. A. lividus is the popular vegetable type in South and Central Europe. Other leafy species are A. viridis, A. tristis and A. spinosus. A. cruentus, A. hypochondriacus and A. caudatus are cultivating for grain purpose. Taxonomists suggested two sections in Amaranthus namely blitopsis which include leafy types and amaranthus which constitutes grain types.

Potato, the third most important food crop in the world after rice and wheat is becoming the main source of food and income in developing countries. India stands second in production and area of potato in world after China leaving behind the Russian Federation. In India, potato is an important component in cereal and vegetable-based cropping systems for its ability to produce high dry matter/ha in short duration and high yield compared to cereals. It is grown as mixed crop, intercrop, relay or sequential crop along with other vegetables, cereals and cash crops.

Potato is originated in South America in the high Andean hills and towards the end of 16th century it was first introduced into Europe by Spanish conquerors. In the early 17th century, it was brought to India by British missionaries or Portuguese traders. Thus, potato developed as a temperate crop and was later distributed throughout the world. Cultivated potato consists of two sub species, Solanum tuberosm ssp. andigena and S. tuberosum ssp tuberosum. Subspecies andigena is the primitive form which is adapted to shorter day length and high altitudes of mountains (above 2000). The presently cultivating tuberosum subspecies is adapted to long or short day condition of tropical regions and lower altitudes (500-200m above MSL) and is believed to be evolved from andigena types through selection.

Morphology and Floral Biology

Potato is herbaceous in nature with erect to prostrate stem. Stem is variably coloured from green, purple or mottled green or purple. Leaves are pinnate which ends in single terminal leaflet. Stolon arise from the portion of the plant below the ground. The stolon bears rudimentary leaves and tip is hook shaped. The sub apical portion at the tip of stolon enlarges to produce tubers. Hence, potato tubers are the modification of underground stem with shortened axis and poorly developed eyes. The leaf scar with a subtended lateral bud having underdeveloped internodes is referred to as the eye of the tuber. Potato is a long day plant. Moderate humidity and long day condition favours flowering. Hence flowering and fruit set is observed in hilly tracts. Inflorescence is produced terminally and is usually cymose. Flowers are bisexual, actinomorphic, and hypogynous with 5 calyx, 5 corolla and 5 stamens. The colour of corolla varies from white, pink, lilac, blue and purple. Pollen shedding is through the pores at the tip of the anthers. Mature fruit is a berry. Self-incompatibility is observed in diploid species and tetraploids are self-compatible.

Root crops are cool season vegetables growing for its fleshy root developed from the tap root and hypocotyl region. They are grown during winter in tropical and subtropical condition, throughout the year in hills and spring-summer in high altitude hills. The major root crops growing in our country include carrot (Umbelliferae), radish (Brassicaceae), beet root (Chenopodiaceae) and turnip (Brassicaceae). Two distinct types viz., asiatic or tropical types and European types present in these root crops except beet root. In beet root, European or biennial types only exist. The tropical types are annuals that does not require chilling treatment for flowering. Therefore, seed production of these types is taken up in plains but European types are biennials and they require vernalization treatment after vegetative phase for flowering. Hence, the seed production of biennial types is possible in hills. The seed productions of Asiatic varieties of root crops are done in Punjab, Haryana, U.P. and parts of Rajasthan and Gujarat. Whereas, the seed production of temperate varieties is done in Saproon valley, Kullu valley, Kalpa valley of H.P., Kashmir valley of J&K and selected location of Northeast region specially Sikkim and Arunachal Pradesh. The seed production of beet root is being done in high altitude hills of J&K. All the crops belonging to this group are similar in the cultural practices though they come under different family. All root crops prefer well drained loose and friable soil. Ridge or bed planting is mainly following in all root crops. They are direct sown crops and does not respond to transplanting. They are highly cross pollinated.

The two methods of seed production following in root crops are seed-to-seed method and root-to-seed method. The first method is followed for the production of truthfully labelled seed or certified seed as the seed yield will be more, but quality remains inferior to the second method. The breeder seed as well as foundation seeds require utmost quality hence root-to-seed is preferred for its production. In this method, the matured roots are harvested and the roots are inspected for its trueness. Only true-to-type roots are selected for replanting in the field for seed production and undesirable ones are discarded. The selection and rouging in seed crop are done on the basis of foliage characters and external and internal root characters (colour, shape, size, flesh colour, core size, pungency, indistinct rings etc.). The roots after selection are prepared for replanting by cutting one-third top (shoot) portion and one-fourth to one-half root cut. the prepared stecklings are replanted to obtain good yield of quality seeds. In case of turnip and beet, after selection, the tap root and tops of the roots are trimmed without injuring the crown portion and is replanted.

Bulb Crops

Bulb crops are cool season vegetables that are grown during the winter months in plains. They are mainly growing for its fleshy leaf bases or fleshy axillary buds or blanched stem and leaves. Major bulb crops that are widely cultivating in our country comprises of onion, garlic and leek. They possess a characteristic odour due to the presence of volatile oil, allyl propyl disulphide (onion) and diallyl disulphide (garlic). They are either seed propagated as in onion and leek or through cloves as in garlic or through bulblets in multiplier onion. Seed propagated bulb crops are annual for bulb production and biennial for seed production. Seed production of bulb crops can be undertaken in plains especially in the central part of the country and southern plains whereas, northern plains hold the problems of Stemphylium and purple blotch during the seed crop and subsequent lower seed yield makes northern plains unfit for seed production. But these areas can be utilized for seed production by following the delayed planting of seed crop and avoiding the climate favouring the diseases.

Onion (Allium cepa)

Onion is a popular vegetable grown for its pungent bulbs. Bulbs are consumed as raw vegetable, as cooked vegetable or in preserved forms. Onion is reported to be originated in Central Asia and Mediterranean region is considered as the secondary centre of origin. It is adapted to a wide range of climate and it is being cultivated in India in three seasons viz., kharif, late kharif and rabi. India is having the largest area under onion cultivation in the world. It is also contributing substantially towards the export of the value-added products from our country. White or yellow onions are commonly using for producing dehydrated products whereas, the red ones are more preferred for direct consumption.

Cole crops refers to vegetable crops originated from a common ancestor, colewort or wild cliff cabbage (B. oleraceae var oleraceae or Brassica sylvestris L.) and they belong to family Brassicaceae. They prefer low temperature for growth and development hence mainly grown as temperate vegetables in temperate and subtropical climatic zones. This group of vegetable includes cabbage, cauliflower, knol khol, brussels sprout, sprouting broccoli, kale, Chinese cabbage etc. They are Mediterranean vegetables since its centre of origin is considered to be Mediterranean region. Later on, it spread into different parts of the temperate and subtropical countries. In India, cultivation of cole crops was started in the hills where temperate climate prevails and in states like Uttar Pradesh, Orissa, Bihar, Assam, West Bengal, Maharashtra and Karnataka as cool season crop. But with the introduction of tropical types which are more tolerant to high temperature cultivation, cole crops are taking new pace in the cropping system of nontraditional areas. Cabbage and cauliflower became the most widely grown cole crops though cabbage, knol khol and curly kale were the first cultivated types and cauliflower came in the mid-way followed by brussels sprout and sprouting broccoli. The genetic designation of different vegetables under cole crops were described by several workers of which Linnaeus (1753) designated all the cole crops under one species, Brassica oleraceae and in 1969, Neuwhof classified them into different forms as B oleracea var capitata L. (cabbage); B oleracea var botrytis L. (cauliflower); B oleracea var gemmifera DC. (Brussels sprout); B oleracea var italica L. (sprouting broccoli); B oleracea var gongyloides L. (knol khol) and B oleracea var acephala DC. (kale).

Cole crops are usually biennial herbs with tap root system and erect stem. They are highly cross-pollinated and temperature sensitive for the production of head and curd. They are classified as early, mid and late varieties based on the temperature requirement and duration. Among this, early varieties are found to be high temperature tolerant and late varieties are low temperature tolerant. The flowering in cole crops requires a chilling temperature of 4-70C for about 6 8 weeks which transforms the juvenile phase into reproductive stage with exceptions of some early varieties of cauliflower and sprouting broccoli. Upon receiving the chilling temperature, the central stalk will develop into reproductive shoot or multiple reproductive shoots will be formed from the curd. The earlier is seen in cabbage, brussels sprout and kale whereas, the latter in cauliflower and sprouting broccoli. The seed production of cole crops belonging to temperate groups are mostly undertaken in temperate hills especially in the hills of Uttaranchal and Himachal Pradesh whereas, seeds of tropical types are produced in plains and lower hills of India.

Leguminous vegetables include wide range of vegetables which can grow in climatic conditions extending from warm to cool weather. They are rich in protein, essential amino acids and fibre hence, recognized as an important component in ensuring nutritional security. They are growing for its pods for vegetable purpose, seeds as pulse or for dual purpose. They are excellent green manures that fix atmospheric nitrogen into soil and cultivation of leguminous vegetable crops is recommended as an integral component in sustainable soil fertility management. Leguminous vegetables include tropical types namely cowpea, hyacinth bean, winged bean, jack bean, sword bean while, cool season legumes include French bean, garden peas, lima bean and broad bean. They are mostly self-pollinated owing to its cleistogamous flowers. Pole or bush types are available in these crops and all are seed propagated. Among the different leguminous crops, French bean, garden peas and cowpea are under commercial cultivation while other leguminous vegetables are region specific in cultivation.

Cowpea (Vigna unguiculata)

Cowpea is a warm season legume grown both as vegetable crop as well as pulse crop. Tender pods are used as vegetable and dry beans are used as pulse. Cowpea is mainly growing for its seeds as pulse than as vegetable crop. The vegetable types bear stringless pods of more than 30 cm long, fleshy pericarp and higher monosaccharide to polysaccharide ratio. Cowpea is believed to be originated and domesticated in West or Central Africa especially Nigeria where diversity was noticed abundant. The cultivated cowpea, Vigna unguiculata is divided into different cultivar groups viz., pulse type as Vigna unguiculata var. unguiculata, Vigna unguiculata var. cylindrica or var. biflora as dual purpose type for fodder and dry seeds and vegetable type as Vigna unguiculata var. sesquipedalis (yardlong bean/string bean/asparagus bean). The cultivar groups, biflora and sesquipedalis are suggested to be originated from ssp. unguiculata in India and South East Asia respectively.