eChapters

Seed Production

Systemized crop production is known as seed production. In seed production adequate care is given from the purchase of seeds upto harvest adopting proper seed and crop management techniques. The benefits of seed production are

• Higher income
• Higher quality seed for next sowing

I. FILL UPS

1. _____ is the growing of plants from meristematic tissue (or) somatic cells of superior plants on nutrient media under controlled conditions.
2. _____ is a group of undifferentiated palnt cells.
3. Explants used is _____ in shape.
4. Meristem forms _____ at its cut end on which a large number of shoot primordia develop.

1. DEFINITION

Micropropagation is the growing of plants from meristematic tissue or somatic cells of superior plants on nutrient suitable media under controlled aseptic physical conditions.

I. FILL UPS

1. _____ are capable of reducing the age induced damages and restoring the seed vigour by controlling free radical reactions.
2. _____ is the process of soaking the low and medium vigour seeds in water with or without adding chemicals to raise seed moisture and drying back for safe storage of seeds.
3. _____ treatment is similar to the moisture equilibration treatment.

60% - Bottlegourd, pumpkin, bittergourd, watermelon

65% - Cotton varieties, bhendi

I. FILL UPS

1. _____ works on the principle based on gravitational force
2. Gamet divider is otherwise known as_____.
3. Gamet divider works on the principle based on_____.
4. _____ method is suitable for seeds requiring working samples upto 10grams and seeds not chaffy and do not bounce and roll.

1. MIXING AND DIVIDING

The main objective of mixing and dividing of seeds is to obtain the representative homogenous seed sample for analysis by reducing the submitted sample to the desired size of working sample.

2. TYPES OF MECHANICAL DIVIDERS

2.1. Boerner divider

The principle involved in mixing and dividing in gravitational force. It consists of a hopper, a cone and series of baffles directing the seeds into 2 spouts. The baffles are of equal size and equally spaced and every alternate one leading to one spout. They are arranged in circle and are directed inward. A valve at the base of the hopper retains the seeds in the hopper. When the valve is opened, the seeds fall by gravity over the cone where it is equally distributed and approximately equal quantity of seeds will be collected in each spout. A disadvantage of this divider is that it is difficult to check for cleanliness (Fig. 1).

Cultivated plants in general including the spice crops observed today have been evolved through selections from their weedy and wild types. Apart from this, there had been another force which could bring the wild forms into cultivated forms named human intervention which is actually the process of domestication. Natural mutations and genetic recombination after selfing and crossing also played a significant role in the entire process. Whereas, natural forces take a long time to come into act, the techniques of plant breeding was devised and fine tuned throughout passing of times, essentially to accelerate the speed of evolution using these natural phenomenons. It was, therefore, mandatory for us to know the modes of reproduction and pollination to create variability in natural population and make all thise happen to what we see today in organised cultivated forms. Moreover, knowledge on reproduction of different plant species helps the breeder to choose the right breeding procedure to be used for crop improvement. Modes of reproduction help to evaluate the genetic constitution of crop plants by determining the extent of homozygosity or heterozygosity which, in turn, are unanimously applicable to any plant. To comprehend modes of reproduction in spice crops effectively, understanding about the floral biology is absolutely necessary. Plant reproduction, a process by which one species gives birth to its offsprings, may be of two types

Molecular markers may be a short DNA sequence that flanks base pairs like SNP, a Single Nucleotude Polymorphism, small fragment or SSR Single Sequence Repeat or an extended site as in the case of microsatellites. Schematic representation of the SSR assay is shown in Fig. 1. Genetic markers have to be easily identified, linked to a specific locus and polymorphic. Molecular markers are subdivided into codominant monolocus and polyallelic markers and the second group contains dominant, polyallelic and biallelic markers. 

Abstract

People dealing with forest tree seeds are very much concern with the collection and supply of pure and quality seed. Assessing genetic purity of tree seed is always a challenge. Besides, studying tree diversity and tree improvement are also the key components of forestry. With the discovery of DNA based molecular markers these tasks have been made easier and authentic. So, the basic concepts behind different molecular markers were the principal concerns of this chapter. It has been focused here about the pros and cons of the markers along with their applicability. The application may be expanded towards hybrid seed testing and even to clonal forestry.

Vegetables have always been looked upon as an essential part of diet and are necessary for human health and well-being. Vegetables supply many kinds of nutrients, e.g., carbohydrates, proteins, minerals, vitamins, fibers, phytochemicals, etc. and also add flavour, colour and crispness to foodstuffs. For developing modern day vegetables, breeders have utilized an understanding of genetic principles and applied them for the improvement of these crops. They often make crosses (hybridization) between a crop plant and a related or wild species (a wide cross) enabling valuable genes to be used for genetic improvement of the crops. Over the last few decades, molecular breeding is supplementing plant breeder’s efforts for accelerated genetic enhancement bringing about a revolution in this area. This approach demands efficient procedures for the routine introduction of foreign DNA into plant genomes and utilizing them for the production of novel types of vegetable. In this chapter, attempts have been made to review most of the available molecular breeding techniques, which can routinely be employed in various aspects of vegetable improvement.

Cultivars are developed by selecting for desired traits or by removing undesirable traits that are controlled by genes (the blueprint for all characteristics in living organisms) from existing varieties or breeding lines. The plant species, varieties and clones are identified or selected by their physical features since olden times. However, these traits would change with respect to environmental and climatic conditions. The biochemical parameters like enzymes, proteins, alkaloids, etc. are also likely to change due to the stages of development of plant tissue. Successful selection strategies are dependent on the breeder’s ability to distinguish genetic effects from environmental effects on a visible or measurable trait. Therefore, the scientists resorted to use ‘genetic marker’, i.e., any stable and inheritable variation that can be measured or detected by a suitable method and can be used subsequently to detect the presence of a specific genotype or phenotype, which otherwise is very difficult to detect. Presently, the breeders and scientists all over the world are pursuing mostly DNA or molecular markers.

Botany

The plant is highly cross pollinated due to heteromorphism and bees being the major pollinators.

Varieties: PKM 1 & PKM 2

Method of seed production : Seed to Seed.

Land requirement : Uproot all the wild and non specific moringa trees if any near the field within a radius of 1500ft. Good drainage facility is must for moringa.

Isolation requirement : A distance of at least 500 M is necessary for seed production.

Season : Early season (July August), Mid season (September), Late season (October -November). Best season is September October, so that flowering starts during summer which facilities better pollination and seed set.

A. General Morphology

2.1 The Root

It is the organ of a plant that typically lies below the surface of the soil.The root is best defined as the non-leaf, non-nodes bearing parts of the plant’s body. However, important internal structural differences between stems and roots exist. Root spices are, rather surprisingly, rare. Some popular examples of spices having roots are: Apium graveolens (Celery), Armoracia rusticana (Horse radish), Coriandrum sativum (Coriander), Ferula asafoetida (Asafetida), Glycyrrhiza glabra (Licorice), Levisticum officinale (Lovage), (Petroselinum crispum (Parsley), etc. All of the mentioned plants have aromatic roots.Roots of gingiberaceae are adventitious and storage roots arise from among the nodes of these scales.

Mulching is a practice of covering the surface of soil with plastics, organic and non-organic materials like straw, grasses, sands, stones (boulders), synthetic plastics, etc. to reduce evaporation and to moderate wide fluctuations in diurnal soil temperature, especially in the root zone environment. It controls external evaporability and reduces energy supply to the evaporating site by cutting off solar radiation falling on the ground. Its main function is limited to control first stage of drying, which helps in improving moisture status and reducing soil temperature (Loy and Wells, 1975), besides checking seedling mortality and improving crop stand. It also suppresses weed-flora and reduces weed competition with crop for water and nutrients making them available in greater quantities for crop plants. Moreover, mulching helps in increasing downward movement of water, and its storage deep in the profiles escapes evaporation due to reduction in thermal gradients and exchange of vapours. The effectiveness of mulches in conserving moisture has generally been found to be higher under more frequency of rainfall, drought conditions and also during early period of plant growth when canopy cover remains scanty.

Vegetables are cheaper source of carbohydrates, proteins, minerals, vitamins and dietary fibers, and hence, assume a place of very great importance in the diet of individuals. All kinds of vegetable are grown almost universally. India occupies the prime position in the production of vegetable crops and is the second largest producer of vegetables in the world. The area and production of vegetables in India have been increasing during the post-green revolution period, and the country is heading towards another revolution in the form of Golden Revolution. However, there is an utmost requirement for genetic improvement of vegetable crops in terms of productivity, qualitative characters and resistance to biotic and abiotic stresses. For any genetic improvement programme, the presence of genetic variation is pre-requisite and mutation is the ultimate source of this heritable variation.

Mutation technique that holds promise of generating a much wider desirable variability than classical breeding has been refined. Out of 2275 mutant varieties worldwide in all crops, as many as 66 mutants are in vegetables. In terms of percentage, China, India, USSR, The Netherlands, The United States of America and Japan account for 26.8, 11.5, 9.3, 7.8, 5.7 and 5.3%, respectively of mutagen varieties in all crops. Such varieties are 3% in case of vegetables. After mutation treatment by a mutagen to a specific plant, DNA alterations (deletion, duplication, inversion, translocation, or transposition of larger DNA fragments, or simple basic pair changes) occur and lead to the DNA sequence change. Hence, new characteristics of that plant are established. In fact, induced mutations supply us with many new plant lines, which may be used as primary materials for crop breeding. Hence, mutation breeding seems to be an effective and ultimate option to improve vegetable crops in the shortest possible time. A large number of new promising varieties in different crops, including vegetables, have successfully been developed worldwide using various mutagens (Chopra, 2005).

Aims and Objectives

It has become evident that in order to achieve the food production targets of the future, a major effort will be required to enhance the seed replacement rates of various crops. This would require a major increase in the production of quality seeds, in which the private sector is expected to play a major role. At the same time, private and Public Sector Seed Organisations at both Central and State levels, will be expected to adopt economic pricing policies which would seek to realise the true cost of production. The creation of a facilitative climate for growth of a competitive and localised seed industry, encouragement of import of useful germplasm, and boosting of exports are core elements of the agricultural strategy of the new millennium.

Biotechnology will be a key factor in agricultural development in the coming decades. Genetic engineering/modification techniques hold enormous promise in developing crop varieties with a higher level of tolerance to biotic and abiotic stresses. A conducive atmosphere for application of frontier sciences in varietal development and for enhanced investments in research and development is a pressing requirement. At the same time, concerns relating to possible harm to human and animal health and bio-safety, as well as interests of farmers, must be addressed.

Globalization and economic liberalization have opened up new opportunities as well as challenges. The main objectives of the National Seeds Policy, therefore, are the provision of an appropriate climate for the seed industry to utilize available and prospective opportunities, safeguarding of the interests of Indian farmers and the conservation of agro-biodiversity. While unnecessary regulation needs to be dismantled, it must be ensured that gullible farmers are not exploited by unscrupulous elements. A regulatory system of a new genre is, therefore, needed, which will encompass quality assurance mechanisms coupled with facilitation of a vibrant and responsible seed industry.

Introduction

Agricultural soils harbour a rich variety of nematode fauna, where they coexist with a myriad of other microorganisms. It has been estimated that one cubic meter soil of highly fertile field may contain as many as 3×10¹⁴ bacterial cells (300 g), 5×10⁸ protozoa (39 g), 1×10⁷ nematodes (12 g) and fungi (400 g), besides other organisms. The nemic fauna comprises of free-living (saprophagous and microbivorous), predatory forms and plant parasites (phytophagous). In addition, certain animal and insect parasitic forms may spend a part of their life cycle in soil. Plant parasitic nematodes (PPNs) exist in poly-specific communities and attack all types of plants- cultivated or wild. They possess a needle-like stylet or spear at their anterior end, which is used to puncture the plant cells and ingest the cell contents. Majority of PPNs dwell in soil and draw their nutrition from plant roots. Only a few forms attack the above ground plant parts such as stem, leaves, inflorescence and seeds. PPNs may be ectoparasites (feed from outside and do not enter plant tissues) or endoparasites (enter inside the plant tissues). The endoparasites spend a part of their life cycle in soil or may come out into the soil to re-infect the other roots. These may be further categorized into migratory or sedentary types. The migratory forms keep on moving, feeding and laying eggs that are scattered in soil or plant tissues. The sedentary nematodes are more harmful and a particular stage of their life cycle infects the plant and establishes a feeding site by modifying plant tissues by their enzymatic secretions. The females of sedentary nematodes usually attain swollen shapes upon maturity and lay hundreds of eggs in clusters.

Plant parasitic nematodes are ubiquitous, and so far, more than 1800 species have been recorded. They are obligate parasites and must feed upon plant hosts, thus, debilitating the plants to some extent. The extent of direct damage by the nematodes to plants depends on several factors viz., nematode density in soil, nature of parasitism (ectoparasite or endoparasite), host susceptibility, cropping pattern, edaphic factors (mainly soil texture and moisture) and ambient climatic conditions (like temperature and relative humidity). Nematodes, by themselves, rarely kill the plants to ensure their own survival. Nematodes also inflict considerable indirect damage to crops by interacting with other plant pathogens (fungi, bacteria and viruses) to cause disease complexes in which nematodes act as incitants, aggravators, or vectors.

7.1 Plant Tissue Culture

Spices have great socio-economic impact in moden food habit of human but loss of resources day by day due to various reasons (industrialization, high population pressure) make the access of these valuable spices limited to people. Low production is general phenomena of common spices due to lack of high yielding varieties. Hence there is a need to improve the propagation method to meet the gap between demand and supply. Conventional vegatative propagation method is common approach for propagation but low multiplication rate, seasonal dependency and susceptible to abiotic stresses and diseases are major problems to achieve the optimum productivity. To overcome these drawbacks scientists have chosen the plant tissue culture technique (a modern approach of biotechnology which deals with the propagation the valuable/endangered plants under laboratory condition). Details about this technique and its role on spices are given below.

Plant Tissue Culture: Basic Components and Objectives

The technique of growing of a whole plant cell, tissue or organ under an aseptic condition in suitable synthetic medium is termed as plant tissue culture (Thorpe, 2007). De-differentiation and re-differentiation are two essential fundamental processes which help in the physio-morphological development of the plant.

Notified Varieties of Different Seed Spices

Leaves are alternate in distichous arrangement; lamina long and linear and leaf sheath covering the internodes; ligule membranous and auricle claw shaped. The inflorescence is a spike and florets are borne on rachis node. Each spikelet has three florets. In two row barely, lateral spikelets become sterile.

Agathi is a fast growing tree upto 10m. tall. Branching is drooping. The stem bark is deeply furrowing forming cork like plates. Leaves are compound, pinnae, 20 to 30 cm. long glabresence to pubescent. Leaflet oblong rounded 18 to 24 pairs of leaflets that are green to dark green mostly, bitter free. Inflorescence is a raceme 4 to 7 cm long and bearing 2 to 5 flowers that are white, red, yellowish and pink. Flowers are 5 to 10 cm long, 3cm wide, curved calyx bell shaped 2 cm long, 2 lobed, fine teeth like serration, fleshy. 

Crop improvement is a continuous process. Crop improvement aims to genetically enhance, enrich and empower the crop plants with superior performance, enhanced yield level, higher quality of food, nutritional, fodder, energy produce and adaptability to climate change and crop growing environments. The intrinsic virtues of the improved cultivars could be sustained mainly through the production of the quality assured seeds endowed with genetic capabilities. The quality assured seed delivery system is through the seed chain from the basic seeds through Nucleus-Breeder-Foundation-Certified seed. Quality seed production system need to be based on genetic, breeding and seed technological protocols including field and laboratory testing to ensure very high genetic purity, true to type and superiority.

Successful onion cultivation begins with vigorous, disease-free seedlings and their timely establishment in the field. Seed quality, nursery-bed preparation, growing medium, nutrition, irrigation, weed control and protection against pests and diseases determine seedling performance. This chapter explains conventional and modern nursery-management practices, including raised beds, plug trays, protected structures, soilless media, fertigation and climate-controlled production. It also discusses seedling selection, hardening, transplanting age, spacing and field establishment. Mechanized, robotic and precision transplanting systems are examined as solutions to labour shortages and uneven planting. Climate-resilient nurseries, sensors and automation can further improve transplant survival, resource efficiency, uniformity, productivity and sustainability.

Nursery management is indeed a very important operation in successful growing of vegetables especially those, which are raised through seedlings in properly managed nursery beds. Raising nursery from seed provides an easy and convenient way to nourish tender young seedlings in a well-managed small and compact area. The nursery area can be conveniently protected from natural hazards, seed borne diseases and insect-pests and can be well managed economically. Some of the vegetable seeds are very costly, for which it is necessary to raise nursery in the most-optimum conditions. In areas where land holdings are small (in hills), nursery raising helps in making economical use of land for about one month and provides enough time to mature for previous crop, harvesting, threshing and land preparation for next crop. Most of the vegetable crops like cabbage, cauliflower, tomato, eggplant, sweet pepper, hot pepper, onion, celery, parsley, lettuce, etc. can successfully be raised through seed by growing them in the nursery and then transplanting in the field.

“A vegetable nursery is a place or an establishment for raising or handling young vegetable seedlings until they are ready for more permanent planting.”

Nursery can be maintained either for personal or commercial purpose, and each has its own advantages and disadvantages. Seedlings raised for own planting will be true to type, sound and healthy. On the other hand, seedlings purchased from outside may not be true to type, vigorous and healthy and may not arrive at destination in due course of time.

I. FILL UPS

1. _____ number of beds measuring 8 m, 0.6 m are sufficient for raising sufficient seedling for one hectare.
2. Seeds are sown singly at _____distance apart in the furrow.

1. SELECTION OF SITE

Select a location where one could ensure semi-shady conditions. Well drained medium-loam soil; rich in organic matter and neutral soil pH. An irrigation source in the vicinity with natural pH water is highly desirable.

I. FILL UPS

1. _____ is the necessary component for vitamins.
2. _____ is vital for seed formation.
3. _____ is essential for protein synthesis.
4. _____ is essential for nut development in peanuts.
5. _____ is the key element for chlorophyll production.
6. _____ is the integral part of amino acids.
7. _____ is essential for seed and cell wall formation.
8. _____ catalyzes several plant processes.

I. FILL UPS

1. The objectionable weed of paddy is _____.
2. The objectionable weed of sunflower is _____.
3. The objectionable weed of oat is _____.
4. The objectionable weed of napier grass _____

1. PRESENCE OF OBJECTIONABLE WEEDS

Objectionable weeds present in the seed crop of the respective species should be removed and destroyed before flowering. They should be verified during field inspection (Table 1). The maximum permitted limit of objectionable weed in seed crop is presented in table 2.

Okra is a tropical vegetable cultivating for its tender fruits. It is one of the major vegetable crops in India which can be grown in summer as well as rainy season. Fruits are rich in vitamin A, vitamin C and minerals like calcium, phosphorus, iodine, iron, and potassium. Dry fruit skins and fibers are valued for manufacture of paper and card boards. The processed product, dehydrated okra plays a considerable role in export earnings. Dried seeds of okra found to have 20-24 percent protein and 13-22% edible oil hence, used for edible oil production. Mucilaginous stem is used for clarification of cane juice for preparing jaggery. Roasted ground seed are used as coffee substitute.

Cultivated okra is believed to be originated in tropical and subtropical Africa and phytogeographic studies indicated that it arose in Abyssinian region (Ethiopia). However, existence of large number of related species with wide variability and dominant characters suggested India as the secondary centre of origin. Some researchers suggested Hindustani centre of origin mainly India, Pakistan, & Burma.

Morphology and Pollination

Okra is an erect robust herbaceous annual with green or green with reddish tinged stem. Leaves are alternate, 3-7 lobbed, palmate, hirsute and serrate. Flowers are solitary, bisexual, axillary, peduncle is 2 cm long, epicalyx is up to 10 and bracteoles narrow and hairy. Calyx is fused with corolla hence falls with corolla after anthesis. Petals are 5, separate, yellow coloured with crimson spot on claw. Staminal column consists of numerous stamens (80-120) which are united to the base of the petals. Ovary is superior, stigma is small, 5-8 fid and deep red. Fruit is pyramidal and oblong beaked capsule, longitudinally furrowed, hirsute or glabrous, dehisce longitudinally when ripe. Seeds are dark green to dark brown, round and tuberculate.

I. FILL UPS

1. _____ crop stabilizes the blood sugar by curbing the rate at which sugar is absorbed from the intestinal tract.
2. Okra is _____ crop.
3. The isolation distance adopted for okra for foundation seeds is _____.
4. Seed rate of okra is _____.

Onion is one of the oldest cultivated plant species. Onion forms the bulb in the first year and the seed in the second year. The leaves arise from a shortened crown stem. The sheaths of the older or outermost leaves enclose the younger ones. The basal portion of the leaves encircles the stem, and thickens to form the bulb. The stem elongates during the second year forming the flower stalk. The onion has a fibrous root system extending to a depth of over one meter, but most of them are found in upper surface about one-half meter area.

Processing extends onion availability and transforms perishable bulbs into stable, marketable products such as dehydrated flakes, powder, paste and pickles. Onion is also rich in phenolic acids, flavonols, anthocyanins and organosulphur compounds associated with antioxidant, antimicrobial, anti-inflammatory and cardioprotective properties. This chapter explains processing operations, quality preservation and the commercial importance of value-added onion products. It also addresses the large quantities of peels, outer scales and trimmings generated during processing. These residues contain quercetin, polyphenols, dietary fibre and other useful compounds that can be recovered for functional foods, nutraceuticals, natural antioxidants, biofuels and biodegradable materials, supporting waste reduction and a circular bioeconomy.

I. FILL UPS

1. Onion is a bulbous _____ herb.
2. Onion is a _____ pollinated crop.
3. Thin white mass within the seed coat is called _____.
4. _____ method is commonly followed for onion seed production.
5. The minimum isolation distance for foundation seed is _____.

1. INTRODUCTION

Green Revolution Technologies (GRT) such as greater use of synthetic agrochemicals like fertilizers and pesticides, adoption of nutrient responsive high yielding varieties, greater exploitation of irrigation potentials etc. have boosted the production output in most cases. However, continuous use of these high energy inputs indiscriminately now leads to decline in production and productivity of various crops as well as deterioration of soil health and environment. The most unfortunate impacts of GRT on Indian Agriculture are imbalance in production, dependency on synthetic chemical fertilizers, increase in secondary and micronutrient deficiencies, increase in pesticide use, unscientific water management and distribution, reduction in productivity, reduction in quality of the produce, extinction of gene pool, environmental pollution and imbalance in social and economic status.

Moreover, today the rural economy is now facing a challenge of over dependence on outside inputs and day-by-day increase in price of these inputs. Further, Indian Agriculture will face the market competition due to globalization of trade as per World Trade Organization (WTO). Thus, apart from quantity, quality will be the important factor. Such varieties of concern and problems of modern Indian Agriculture gave birth to various new concepts of farming such as organic farming, natural farming, biodynamic agriculture, do-nothing agriculture, eco-farming, etc. Therefore, for sustaining the productivity of the crop, maintaining the soil health and healthy ecosystem, there is a need for adoption of an alternative farming system, may be the organic farming.

Organic farming is not new to Indian farming community. Several forms of organic farming are being successfully practiced in diverse climate, particularly in rainfed, tribal, mountain hills and resource poor areas of the country. It safeguards/improves quality of resources and environment. It is labour intensive and provides an opportunity to increase rural employment.

For a product to be called and labelled “organic”, it should have been produced from start (seed) to end (the produces, the consumer is buying) in an organic way. International Federation of Organic Movement (IFOAM) has clearly laid down the condition that in order to get organic certification to the produces, the seed used for sowing should also have been produced organically. To enter into organic agriculture, timely research has been warranted to study the strategies and efficacies of organic seed production to fulfill the global organic seed demand.

Organic seed production system involves use of organic seed quality enhancement treatments, integrated organic nutrient management practices viz., organic manures, green manures and biofertilizers etc., and integrated organic plant protection viz., agronomic practices, crop rotation, growing border/trap crops and use of botanicals, biopesticides and biocontrol agents apart from encouraging natural parasites, predators and parasitoids etc.

Organically produced seeds are obtained by adapting the organic crop production system. Organic seed is a critical input but specialised efforts to produce organic seeds are not adequately and systematically followed. Accredited organic farming standards recognises the organic seed production as a prerequisite to organic farming. Seed health is important in organic seeds since seeds are not treated with chemical fungicides.

Onion (Allium cepa L.) is one of the oldest and most widely cultivated vegetable crops, with a history of domestication extending over five thousand years. Valued for its culinary, medicinal, nutritional and cultural importance, it has travelled across continents through migration, trade and agricultural exchange. Its broad ecological adaptability has enabled cultivation in temperate, subtropical and tropical regions. The chapter examines probable centres of origin, historical evidence of cultivation, domestication and geographical dispersal. It also explains onion’s nutritional and therapeutic significance, global production status, genetic diversification and role in human civilization, providing a historical foundation for understanding modern onion improvement and cultivation.

What is seed?

“A seed is an embryo, a living organism securely fixed in the supporting or the food storage tissue.”

A true seed is defined as “Structurally a true seed is a fertilized matured ovule, consisting of an embryonic plant, a store of food and a protective seed coat, a store of food consists of cotyledons and endosperm”. In broad sense, “seed is a material which is used for planting or regeneration purpose”. Scientifically, “seed is a fertilized matured ovule together covered with seed coat is called seed” or “it is a propagating material i.e., part of agriculture, sericulture, silviculture and horticultural plants used for sowing or planting purpose”.

The business of Seed Technology is to protect this biological entity and look after its ‘welfare’, while the focus of Food Technology is on the second component – the supporting tissue.

I. FILL UPS

1. Pearl millet is a_____ pollinated crop.
2. An example for protogyny is_____.
3. The isolation distance for foundation seed production of pearl millet is _____.
4. The spacing adopted for pearl millet is _____.
5. Seed yield of pearl millet is_____.
6. The moisture content of pearl millet seed during storage should be_____.
7. The planting ratio for pearl millet hybrid seed production is _____.
8. Presence of fertile counter part of female plants in the female rows is called _____.
9. In some female plants, fertile anthers may be hanging out only from a portion of the panicle. Such plants are called _____.
10. _____ technique is used for hybrid seed production of pearl millet.

Botany : Pea is a self pollinated crop. Time of anthesis is 5.45 to 6.30 AM. Time of dehiscence is 5.45 to 7.30 AM. Duration of pollen futility is on the day of anthesis to 24 hr there after. Duration of stigma receptivity is 48 hr before and 24 hr after anthesis.

Method of seed production : Var: Open pollination ; Hybrids: Emasculation and Pollination

Varieties / Hybrids

Early varieties : Arlcel, Matar Ageta 8, Early Badger, Pant Uphar, Hisar Harit, P.M. 2, Jawahar Matar 4, Meteor, Alaska, Lucknow Boniya, Pant Sabzi Matar 3, Pant Matar 2, VL Ageti Matar 7, Asauj.

Mid season varieties: Bonneville, Lincoln, VL 3, Shalimar Matar, Solan Nirog, Jawahar Matar 1, Pant Uphar, p88, Khapar Kheda, JP. 83, JP71, NP. 289, T19

Late varieties: Kinnauri

TROPICAL AND SUBTROPICAL FRUITS

2.1 Mango
 

Mango is considered to be an allopolyploid. The chromosome number is 2n =40. The polyembryonic seedlings of cv. Vellaikolumban, earlier reported to be 2n= 80 was later shown to be a diploid. In Canary Island, a putative tetraploid viz Gomera-1 has been identified.

Floral biology

Flower starts opening early in the morning from 4-7 a.m. and maximum flowers open between 9.30-10.30 a.m. and complete 11 a.m. Dehiscence of anthers takes place at 11.30 a.m. and it continues up to 3.45 p.m. The pollen grains are oval, or triangular or oblong. Stigma becomes receptive even 18 hours before flower opening. Mode of pollination is entomophily; nectar is present to attract the insects.

The flowering duration is usually of short i.e. 2 to 3 weeks. The mango inflorescence or panicle bears mainly two types of flowers - male and perfect. The number of flowers per panicle varies between 1000 to 6000 depending upon the variety and climatic factors. The percentage of perfect flowers varies between 0.74 per cent in Rumani, 16.41 to 55.7 per cent in Neelum and up to 69.8 per cent in Langra.

Botany: Periwinkles (Catharanthus roseus) belonging to the family Apocynaceae are evergreen perennials that produce bright blue flowers. They grow best in well-draining, moist soil and partial sun to full shade. They work well as sprawling groundcover or border plants and bloom from early spring to early fall. Periwinkles are often grown as transplants, but they can be started from seed about 10 weeks before the last frost with the proper care.

Varieties: Nirmal, Dhawal and Prabal

Soil and climate: It is suited to all types of soil and tropical climatic conditions. Well distributed annual rainfall of 100 cm or more is ideal for raising as a rainfed crop.

Seeds and planting: Propagated through seeds either by direct sowing or throughtransplanting.

Seed rate: 2.5 kg/ha : Direct sowing

0.5 kg/ha : Through seedlings

45 - 60 days old seedlings are transplanted at a spacing of 45 x 20 cm during June - July or September - October.

Pre sowing seed treatment: Fortification with GA3 100 ppm (or) moringa leaf extract 2% for 16 h (or)KH₂PO4 2% for 16 hours

Age of the seedling for transplanting: The seeds are first sown in primary nursery (pot) and after 16 day the seedlings are transplanted to raised nursery and after 24 days transplanted to main field.

4.1 Phases of technology transfer
To translate laboratory research into market ready product, it is important to understand steps or phases involved in this technology transfer process. The process typically includes six main phases, each with a different role (figure 9).
4.1.1 Research
Technology transfer begins with research, the critical stage of exploring and discovering novel knowledge, technologies, or innovations that may have potential commercial applications. The research phase can occur in academic institutions, research organizations, or private sector companies, often funded by public or private sources. Thorough research ensures that the technologies being transitioned are scientifically and technically robust, thereby increasing their potential for market success. Therefore, the research phase involves creating and evaluating new ideas, confirming their feasibility, and identifying IP prospects, all of which are key components of TT success.
4.1.2 Innovation disclosure
Innovation disclosure is the first formal step where the innovation is reported to the appropriate TTO or IP management team. This process ensures that the institution or organization recognizes the innovation and evaluates its potential for protection and commercialization. It includes following information:

Botany

Phyllanthus is an herb common to Central and Southern India. It can grow to 30–60 centimeters in height and blooms with many yellow flowers. All parts of the plant are employed therapeutically. It is a small erect annual herb 10-60 cm tall widespread in tropical areas. Leaves are small elliptical - oblong and flowers are whitish green & minute. It bears ascending herbaceous branches. The bark is smooth and light green. The fruits are tiny, smooth capsules containing seeds.Kizhanelli is a circum-tropical weed, thriving well under tropical conditions. In India, it grows abundantly up to 700m altitude in Punjab, Haryana, Uttar Pradesh, Bihar, West Bengal, Orissa, Madhya Pradesh, Maharashtra, Andhra Pradesh, Karnataka, Tamil Nadu and Kerala.

Major constituents – Phyllanthin (0.4-0.5%) and Hypophyllanthin

Uses- Hepatitis B and Jaundice

Physical purity test determines the proportion of pure seed, other crop seed, weed seed, inert matter, moisture and germination components in the seed lot by weight in percentage in accordance to prescribed Seed Standards. It thus, helps in improving the plant stand, raising a pure crop by eliminating other crop seeds, weed seeds and disease free-seeds. Seed must not be sold until the percentage of pure seed, other crop seed, weed seed, and inert matter are in accordance to seed physical purity standards. Physical purity test is required for seed certification or seed law enforcement and it is a pre-requisite for ger- mination test.

Harvesting is one of the agronomic management practices that require technical knowledge on maturation of the crops. This knowledge is much important in seed production than in commercial production.

Physiological maturation

• It is the stage of accumulation of maximum dry matter within the seeds. The moisture content of the seed at this stage will be in decreasing order (25-30%) and is expressed with maximum dry weigh of seed, germination and vigour potential. The physiological maturation is represented for individual seed and this maturation will not be the same for the population, due to differential flowering habit.
• The attainment of physiological maturation is represented by sigmoid curve of growth pattern. The physiological maturation can be represented both as duration and visible symptoms.

Abstract

The availability of the quality seed is the first and the most important requirement which is of great relevance in tree improvement programme. Unlike agriculture, the forestry seeds have great variation in size, shape, dormancy, viability, moisture content, etc. It needs special techniques for collection, handling, processing and storage of the seeds of the large number of forest species. A strong commitment to improving tree seed technology is required for full utilization of seed supplies with the help of recent advances in seed physiology. Substantial improvements have occurred as a result of seed technology research offering the potential for major advances in traditional seed production and distribution systems.

A TTO fills the important intermediary position of linking research institution with industry so that innovations and new ideas can reach market. To be able to facilitate that effectively, the institutions must have well-defined R&D policies that include Sponsored R&D, Collaborative R&D, and Grant-in-Aid R&D. These ensure that the research remains properly managed, regulatory compliance assured and aligned with institutional priorities. This chapter has discussed about key policies that can help in successful operation of TTO while aligning institution’s mission and interests Various core policies that help in successful technology transfer include:
• Intellectual property policy
• Technology transfer policy
• Revenue sharing policy
• Startup/Spinout/Venture creation policy
• Conflict of interest/Conflict of commitment policy
Each policy has specific objectives. These policies collectively ensure a clear and consistent framework for technology commercialization.
11.1 Intellectual property policy
An IP policy is a formal document that provides guidelines for managing IP by an organisation. A strong IP policy build trust by ensuring efficiency and transparency. Other benefits of IP policy includes creation of environment to encourage innovation and commercialisation of IP. It also lays foundation to set out the rules for public-private partnerships while addressing issues among various stakeholders and ensures compliance. IP Policy is not law, but a proposed course of action.