eChapters

Early passage cell lines are relatively unstable and go through a period of adaptation to culture. However, between 5^th and 35^th generation (human diploid fibroblasts), the culture is fairly consistent although it varies from one cell to another cell. Finite cell lines should be preserved after adaptation but before senescence (getting older). Cells should be preserved to protect cell line instability, availability in case of contamination, incubator failure or accidental loss.

Frequency Distribution: A tabular presentation of the data in which the fre- quencies of values of a variable are given along with them is called a frequen- cy distribution. Two types of frequency distribution are available

1. Discrete Frequency Distribution or Ungrouped Frequency Distribution
2. Continuous Frequency Distribution or Grouped Frequency Distribution

Objective (a): Prepare a discrete frequency distribution from the following sentence in English: “Today there is hardly a phase of Endeavour which does not find statistical devices at least occasionally useful.”

Theory: This problem relates to discrete frequency distribution i. e. a frequency distribution which is formed by distinct values of a discrete or continuous variable.

8.1 Sub Cloning
    
8.2 Cloning into a Yeast Artificial Chromosome (YAC)
    
8.3 DNA Libraries

Telemedicine is a rapidly developing application of clinical medicine wherein medical information is transferred via telephone, the Internet or other networks for the purpose of consulting, and sometimes remote medical procedures or examinations. Telemedicine may be as simple as two health professionals discussing a case over the telephone, or as complex as using satellite technology and video-conferencing equipment to conduct a real-time consultation between medical specialists in two different countries. Telemedicine generally refers to the use of communications and information technologies for the delivery of clinical care.

Care at a distance (also called in absentia care), is an old practice which was often conducted via post; there has been a long and successful history of in absentia health care, which, thanks to modern communication technology, has metamorphosed into what we now know as modern telemedicine.

The terms e-health and tele-health are at times wrongly interchanged with telemedicine. Like the terms “medicine” and “health care”, telemedicine often refers only to the provision of clinical services while the term tele-health can refer to clinical and non-clinical services such as medical education, administration, and research. The term e-health is often, particularly in the UK and Europe, used as an umbrella term that includes tele-health, electronic medical records, and other components of health IT.

1.     Maintaining Embryonic Stem (ES) Cells
    
2.     Establishing ES Cells
    
3.     Freezing of ES Cells
    
4.     Thawing of Frozen ES Cells
    
5.     Production of Transgenic using ES Cells
    
6.     MTT Assay for Cell Viability

A nimal disease monitoring describes the ongoing efforts directed at assessing the health and disease status of a given population. The disease can be a specific infectious disease/diseases or health in general, whereas the monitoring activities are the routine recording, analyses and distribution of information related to the disease (or health). The term disease surveillance is used to describe a more active system and implies that some form of directed action will be taken if the data indicates a disease level above a certain threshold.

In today’s click-and-access kind of world, Information Technology is finding applications in every field and animal disease monitoring & surveillance is no exception. The various technologies which are applied are Geographic Information Systems, Global Positioning System, database management, computer aided design (CAD), computer network technologies, artificial intelligence (AI), expert systems etc.

Objective: The distribution of age of males at the time of marriage was as follows

Introduction
Assisted reproductive technology (ART) refers to treatments and procedures in the field of reproductive biology that are utilised to achieve pregnancy by artificial or partially artificial means.
Artificial insemination
Artificial insemination (AI) is the earliest form of assisted reproductive technology. The technology has been of immense relevance in enhancing production and health features through the appropriate use of better male animals, as well as in eliminating certain venereal illnesses.
• Semen collection: The man is typically allowed to mount a phantom during the process. In cattle, an artificial vagina is used)
• Semen evaluation: Evaluating semen is crucial before processing. The most basic semen evaluation involves examining the concentration, progressive motility, and morphological normality of the spermatozoa)
• Semen dilution, packaging and freezing: Following examination, the semen is ready for dilution and packaging. Spermatozoa require storage mediums that are particular to each species. After packaging, the semen dosages must be properly maintained until insemination. Cryopreservation is typically used for cattle.
• Insemination: Insemination should occur during the female’s oestrus, when she is receptive to copulation.
Multiple Ovulation and Embryo Transfer (MOET)
The main processes are to stimulate a donor female to create suitable embryos, then remove the embryos from her and transfer them to unmated recipient females at equivalent post-ovulatory reproductive phases, ensuring that the donor’s embryos are carried to term in the recipients’ uteri. Multiple ovulations are caused by superovulation or superstimulation, a procedure that increases the number of ovulations in a donor beyond what is normal for a given species. Embryos are recovered 6-8 days following the start of oestrus. Most embryos are transferred during the late morula or blastocyst stage.

The tentative diagnosis of viral diseases of livestock can be made by clinical symptoms alone. The clinical diagnosis is substantiated by the laboratory to obtain a precise, correct and prompt diagnosis. The success however depends on the collection of suitable materials in proper transport medium at a particular stage of disease condition and its dispatch to the laboratory in a manner that little or no alterations occurs in the specimen. This would facilitate the isolation and identification of the causative viral agents easily.

Starting from the middle of the 20^th Century, information has become a commodity. Those best able to access information will be more successful than their peers in their future careers. Technological competence will be as important as medical competence to practice at the highest levels. Advances in computer technology have made access to veterinary and medical databases much simpler than in the past. It is quite likely that in the future, it will be a malpractice for practitioners not to access online information essential to the treatment of their patients. Wireless digital transfer of information will make access to necessary information independent of time and place.

Veterinary medicine and educational delivery are becoming an increasingly international market. Those who are prepared to deliver material in this manner will have a competitive advantage. Use of digital technologies will allow veterinary colleges to share information, including class material, more efficiently. This will enable each college to market specific educational information for students at other veterinary colleges or continuing education modules to graduate veterinarians around the world. On-line certificate programmes in specific veterinary topics will be developed. Use of computer technologies can enrich the teaching environment. By digitizing library, classroom, and laboratory visual images, students can access these materials at any time or place, freeing them from library and classroom schedules. Medical colleges have found that students heavily use these image resources. Computer technologies provide both horizontal and vertical integration of the professional curriculum, allowing students to explore information to the depth at which they are comfortable, without regard to their “year of study” in a traditional “linear” curriculum. Allowing students to see future applications of current learning material provides additional incentive to master basic material. Computers are a tool, just like a textbook, notebook, or calculator.

Bovine
 
1.    PPR    Eye, mouth, rectal swab in PBS on ice, 10 ml blood  in anti-coagulant at height of temperature, Prescapular lymph node, spleen, piece of intestine on ice, lung, liver, spleen, tonsil in 10% neutral formol saline solution.

Computers take on an increasingly important role in today’s world. In this regard, their utility as tools for imparting high quality professional knowledge to veterinarians is much needed. e-learning covers a wide set of applications and processes, including computer-based learning, web-based learning, virtual classrooms, and digital collaboration. The delivery of content could be via electronic media, including the internet, intranets, extranets, satellite broadcast, audio/video tape, TV and CD-ROM. Information and Communication Technology (ICT) in the form of on-line teaching, face-to face video-cases and video-performances has recently been applied in the veterinary profession all over the world. ‘m-learning’ is the new buzz word almost indicating learning “anywhere” using mobile phones and Bluetooth or WiFi technologies. However, in the veterinary profession, connectivity is an issue as high quality network service is lacking in most remote areas. Some other terms frequently interchanged with e-learning are online learning, online education, distance education, distance learning, technology-based training, web-based training and even computer-based training (generally thought of as learning from a CD-ROM). e-learning is a broad term used to describe learning done at a computer.

Processing

On arrival in the laboratory, the specimens are processed immediately or refrigerated until processed. For inoculation into animal/cell culture/embryonated eggs (EE), swabs are shaken in fluid medium, faeces are dispersed in fluid medium and tissue specimens are homogenized in a high speed blender/pestle and mortar with the help of an abrasive like powdered glass or sand. Good quality hard sand should be properly washed in distilled water, treated with HCl, washed thoroughly, dried and sterilized before use. Generally the infective tissue is homogenized in a few ml of buffer usually having a pH of 7.2 to 7.3 to make about 10% tissue suspension. The suspension is centrifuged at about 1000g for 10-15 minutes to sediment the tissue debris and coarse materials.

Moments

In statistics, moments are measures of the shape and variability of a data set. They are used to describe the location and dispersion of the data. There are several types of moments that can be calculated, each providing different information about the data set.

Moments is a term which refers to a measure of force with respect to its tendency which provides rotation. The strength of the tendency depends upon the amount of force and distance from the point of origin where force is exerted.

So total moments of all the forces about origin, if total moment is divided by the total force, which is called moment actually arithmetic mean for frequency distribution.

Hence, arithmetic mean is called first moment about the origin

• Arithmetic mean of various powers expressed as deviation from mean in any distribution is called the moment of the distribution.

Principle

The infectivity of a virus may be neutralized by specific antibody when they are mixed and incubated. In some cases, the addition of homologous or guinea pig complement to the mixture enhances neutralization, particularly when early antiserum is used. Serum must be inactivated by heating at 560C for 30 minutes to inactivate complement or to remove non-specific virus inhibitors. Serum-virus mixtures are inoculated into appropriate cell cultures, which are then incubated until the only virus controls develop cytopathic effects. Antibody by neutralizing the infectivity of the virus, protects the cells against virus destruction.

A. Definitions of probability

1. A probability relies on having an understanding of the theoretical model defining the set of all possible outcomes of a trial; we evaluate the probability solely on the basis of this model, without recourse to performing the experiment at all. It is often called an a priori probability. So, for example, we know that there are two equally likely outcomes when an unbiased coin is tossed: either a head or a tail. This is the model from which we can deduce that the probability of a defined event, obtaining a head, say, is 1/2 = 0.5.

2. The next approach to defining a probability, and the one commonly used in statistical inference, is to regard a probability as the proportion of times a particular outcome (the event) will occur in a very large number of ‘trials’ or ‘experiments’ performed under similar conditions.

The result of any one trial should be independent of the result of any other trial, so whether or not the event occurs in any one trial should not affect whether or not the event occurs in any other trial.

5.1 Graphical Representation of Data

Diagrams are generally useful for the purpose of publicity and propaganda. No statistical conclusion can be drowning as these provide only an approximate and rough expression about the phenomenon. Thus for a more rigorous and improved representation of numerical statements graphs and charts are used.

Graphs are generally drawn with the help of two perpendicular lines known as x-axis and y-axis. The following are the various types of graphs, which are in common use:

6.1 Correlation

The term correlation indicates the relationship between two such variables in which with changes in the values of one variable, the values of the other variable also change. In short, the tendency of simultaneous variation between two variables is called correlation.

FOR GROUPED DATA

Objective: Data regarding lives of two new models of refrigerators collected in a recent survey are

8.1 Introduction

The normal distribution was discovered by De Moivre in the year 1733. Normal distribution is probably the most important of all theoretical distributions for the reason that so many physical measurements and natural phenomena have observed frequency distributions which very closely resemble the normal distribution.