eChapters

1.     Animal Biotechnology Laboratory
    
2.    Growing Cells with Serum-Basal Medium Eagle (BME), MEM
    
3.     Media Preparation and Sterilization
    
4.     Contaminations in Animal Tissue Culture

A Hospital Management System not only provides an opportunity to the hospital to enhance their patient care but can also increase the profitability of the organization. These kinds of applications enable hospitals and doctors to serve their patients better. Computers are being extensively used for routine administrative purposes by health care professionals.

In veterinary medicine, hospital management software is designed for all types of practices, ranging from small, single-vet ones to large ones with multiple locations and vets, and covers a wide range of hospital administration and management processes. It is an integrated end–to-end management system that provides relevant information across the hospital to support effective decision making for patient care, administration & critical financial accounting in a seamless flow. Specific areas of application include scheduling of appointments, registration of cases including client and patient data, case history and electronic medical records, health care reports, reminders, inventory controls, reports and accounting.

At country level, there is major paradiam shift from cropping systems towards farming system research in XI Plan period. The mandate of Project Directorate for Cropping Systems Research, Modipuram stands changed with focus on Farming Systems and re-named as Project Directorate for Farming Systems Research. w.e.f. 1.4.2009. The All India Co-ordinated Research Project on Cropping Systems with its 69 on-station and on-farm centres representing different agro-climatic zones of the country also stands re-named as AICRP on Integrated Farming Systems. As such, the much needed methodologies and procedures for planning, monitoring and interpretation of farming systems related data are not readily available. Therefore, some methodologies and procedures which can be adopted to execute Farming Systems Research for drawing meaningful conclusions are discussed here.

2.1 What is a frequency distribution?

A frequency distribution shows the frequencies of occurrence of the observations in a data set. Often the distribution of the observed data is called an empirical frequency distribution, in contrast to the theoretical probability distribution determined from a mathematical model.

The class interval is a term used in statistics when we are given a continuous series. Class means a group of numbers in which items are placed such as 0-10, 10-20, 20-30, etc. Class interval refers to the numerical width of any class in a particular distribution.

2.2 Relative frequency distributions

Although creating a frequency distribution is a useful way of describing a set of observations, it is difficult to compare two or more frequency distributions if the total number of observations in each distribution is different. A way of overcoming this difficulty is to calculate the proportion or percentage of observations in each class or category. These are called relative frequencies and each is obtained by dividing the frequency for that category by the total number of observations (column 3 of Table 2.1). The sum of the relative frequencies of all the categories is unity (or 100%) apart from rounding errors.

Correlation coefficient

We measure the degree of association between two variables by calculating Pearson’s product moment correlation coefficient, usually just called the correlation coefficient.

It was developed by Karl Pearson from a related idea introduced by Francis Galton in the 1880s, and for which the mathematical formula was derived and published by Auguste Bravais in 1844. In 1896, Karl Pearson published his first rigorous treatment of correlation and regression in the Philosophical Transactions of the Royal Society of London.

The linear correlation coefficient (sometimes called Pearson's Correlation Coefficient), commonly denoted, is a measure of the strength of the linear relationship between two variables.

• We say that we have perfect correlation if all the points lie on the line; in this case, the value of the correlation coefficient takes one of its extreme values, either +1 or −1 (Figure 7a and 7b).

• We have positive correlation if the sign of the correlation coefficient is positive; then there is a direct relationship between the two variables so that as one variable increases in value, the other variable increases (Figure 7a) or there is a tendency for it to do so.

• We have negative correlation if the sign of the correlation coefficient is negative; then there is an inverse relationship between the two variables so that as one variable increases in value, the other variable decreases (Figure 7b) or there is a tendency for it to do so.

• We have no linear association (i.e. the variables are uncorrelated) if the correlation coefficient is zero; then there is a random scatter of points with no indication of a linear relation between the variables (Figure 7c.). Note that a non-linear relationship between the variables can also give a correlation coefficient of zero.

A probability distribution is a statistical function that describes all the possible values and likelihoods that a random variable can take within a given range. This range will be restricted between the minimum and maximum possible values.

The probability distribution shows how the set of all possible mutually exclusive events are distributed, and can be presented as an equation, a chart or a table.

A variable which can take different values with given probabilities is called a random variable.

There are numerous probability distributions which may be distinguished by whether the random variable is discrete, taking only a finite set of possible values, or continuous, taking an infinite set of possible values in a range of values.

A discrete random variable with only two possible values is called a binary variable, e.g. pregnant or not pregnant, diseased or healthy.

Probability distributions for discrete random variables The probability distribution for a discrete random variable y is the table, graph or formula that assigns the probability P(y) for each possible value of the variable y.

14.1 Computer

Computer is an electronic device designed to accept and store data, process them and produce meaningful results under the direction of detailed step by instructions.

In 1822, Charles Babbage, a professor of Cambridge University designed a machine called an “Analytical Engine”, which could store data and perform arithmetic operations and printing out results. He is known as “Father of Computer”.

14.1.1 Basic anatomy of computer

The word Anatomy of a computer means inner structure of the computer. The study of anatomy of computer is necessary to acquire the knowledge and functioning of its inner components. Thus, essentially a computer consists of the following three main units

Buffaloes have been classified in two main categories namely riverine and swamp buffaloes depending upon variation in their habitat and genome structure. River buffaloes are generally large in size mostly with curled horns and are mainly found in India, Pakistan and some of the west Asian countries, prefer to enter clear water and have 50 (2n) numbers of chromosomes and are primarily used for milk production and also used for meat production and draught power. Swamp buffaloes are stocky animals with marshy land habitats and have 48 (2n) numbers of chromosomes. They were primarily developed for draught power in paddy fields and haulage and are also used for meat and milk production. Swamp buffaloes are mostly found in south East Asian countries and few animals are also available in north eastern states of India.

Functional Genomics

• Expressed genes can be identified in a cDNA library
• A microarray can identify genes that are transcribed
• The coordinate regulation of many genes is revealed by a DNA microarray analysis

Proteomics

The proteome is much larger than the genome

2D gel electrophoresis is used to separate a mixture of cellular proteins

Mass spectrometry is used to identify proteins

Protein microarrays can be used to study protein expression and function

Invertebrate Development

The early stages of embryonic development determine the pattern of structures in the adult organism

The study of Drosophila mutants with disrupted development patterns has identified genes that control development

The generation of a body pattern depends on the positional information that each cell receives during development

The gene products of maternal effect genes are deposited asymmetrically into the oocyte and establish the anteroposterior and dorsoventral axes at a very early stage of development

Gap, pair-rule, and segment-polarity genes act sequentially to divide the Drosophila embryo into segments

The expression of homeotic genes controls the phenotypic characteristics of segments

The developmental fate of each cell in the nematode Caenorhabditis elegans is known

Heterochronic mutations disrupt the timing of developmental changes in C. elegans

Introduction

Poultry rearing in North East is very popular as backyard venture and is an inseparable part of poor tribal people of this region.  Majority of birds reared in backyard yard system are indigenous nondescript in which growth and production is very low (60-70 eggs per bird per annum; 2.5 kg. and 1.5 kg. body weight per bird in males and females, respectively). Due to this reason, the per capita availability of egg and meat is too low as compared to recommended allowance, particularly in rural areas. Moreover, the agriculture of N.E. region is characterized by monocropping system. The system of cropping is not suitable for providing round the year farm income. Therefore, most of farmers of this region rearing pig, goat and poultry in the back yard of their house to support their livelihood throughout the year. The N.E. region is most suitable for rural poultry production having plenty of natural foods i.e. good greenery through out the year, insects and other food resources.  Rural poultry production also has an appreciable advantage of being easy to raise, low input and higher reproduction. Rural poultry farming utilizing surplus coarse grains, kitchen waste and greenery and converting them into protein rich human food and valuable manure.  The manure obtained from poultry having more essential nutrients Nitrogen (three times the cattle manure), Phosphorus, Potash, Magnesium, Sodium and Calcium plus trace minerals, which are very essential for soil fertility and texture.

Gene Frequency

A population, in the genetic sense, is not just a group of individuals, but a breeding group and the genetics of a population is concerned not only with the genetic constitution of the individuals but also with the transmission of the genes from one generation to the next. In the transmission the genotypes of the parents are broken-down and a newest of genotypes is constituted in the progeny, from the genes transmitted in the gametes. The genes carried by the population thus have continuity from generation to generation, but the genotypes in which they appear do not. The genetic constitution of a population, referring to the genes it carries, is described by the array of gene frequencies, that is, by specification of the alleles present at every locus and the numbers of proportions of the different alleles at each locus. If for example, A1 is an allele at the A locus, then the frequency of A1, is the proportion or percentage of all genes at this locus that are the A1 allele. The frequencies of all the allele at any one locus must add up to unity, or 100 percent. 

Heterosis, hybrid vigor, or outbreeding enhancement, is the improved or increased function of any biological quality in a hybrid offspring. The adjective derived from heterosis is heterotic.Heterosis is the occurrence of a superior offspring from mixing the genetic contributions of its parents. These effects can be due to Mendelian or non-Mendelian inheritance.

Introduction

The term "nano" is used to define one that has a size in the nanometer range. Nanotechnology is an interdisciplinary field of medicine, chemistry, physics, and biology that has emerged during the past few decades as a potential area of study. The goal of this interdisciplinary technology is to transform molecules into entities, then do the opposite. Nanobiotechnology is the term for the fusion of nanotechnology with biotechnology. In nanobiotechnology nanoscale principles and techniques are used to explore biosystems and generate novel biomaterials. Biosensing, cell labelling, and molecular imaging can all gain benefit from nanostructure materials in life science, health and biomedicine.

Introduction
• Cytogenetics is essentially a branch of genetics, but is also a part of cell biology/ cytology (a subdivision of human anatomy), that is concerned with how the chromosomes relate to cell behavior, particularly to their behaviour during mitosis and meiosis. Techniques used include karyotyping analysis of G-band chromosomes, as well as molecular cytogenetics such as fluorescent in situ hybridization (CGH). Cytogenetic approaches to studying chromosomes and their relationship to human disease have improved greatly over the past several decades.
As a mature enterprise, cytogenetics now inform genomics, disease and cancer genetics, chromosomes evolution and the relationship of nuclear structure to function. Cytogenetics involves testing samples of tissue, blood or bone marrow in a laboratory to look for changes in chromosomes including broken, missing, rearranged or extra chromosome. Cytogenetics may used to help diagnose a disease or condition, plan treatment or find out how well treatment is working.
Chromosomes
Chromosomes are the rod shaped; dark-stained bodies seen during metaphase stage of mitosis. The chromosomes are the nuclear components of special organization, individuality and function. They are capable of self-reproduction and play a vital role in heredity, mutation, variation and evolutionary development of the species. Chromosomes are thread-like structure located inside the nucleus. Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. The number of chromosomes is constant for a particular species. Therefore, these are of great importance in the determination of the phylogeny and taxonomy of the species. The number of chromosomes varies from species to species but the number remains constant in a species. But sometime, due to certain accidents or irregularities at the time of cell division, crossing over or fertilization some alteration in morphology and number of chromosomes takes place. Humans have 23 pairs of chromosomes-22 pairs of numbered chromosomes, called autosomes, and one pair of sex chromosome, X and Y. Each parent contributes one chromosome to each pair so that offspring get half of their chromosomes from mother and half from their father.

DNA replication. The double helix is unwound and each strand acts as a template. Bases are matched to synthesize the new partner strands.

DNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is “replication” in that each strand of the original double-stranded DNA molecule serves as template for the reproduction of the complementary strand. Therefore, following DNA replication, two identical DNA molecules have been produced from a single double-stranded DNA molecule. Cellular proofreading and error toe-checking mechanisms ensure near perfect fidelity for DNA replication.

In a cell, DNA replication begins at specific locations in the genome, called “origins”.

Unwinding of DNA at the origin, and synthesis of new strands, forms a replication fork. In addition to DNA polymerase, the enzyme that synthesizes the new DNA by adding nucleotides matched to the template strand, a number of other proteins are associated with the fork and assist in the initiation and continuation of DNA synthesis.

Hill cattle in Uttarakhand are multipurpose animals. They are used for milk, draft, manures and ceremonial purposes. Improvement for these attributes depends on definition and subsequent selection for various traits. Based on the criteria for improvement and detailed literature analysis, it is believed that the hill cattle of Uttarakhand is one of the potential population for milk in the state specially in the remote and high altitude areas.. Hill cattle have also been used for dairy improvement as a dam breed for crossbreeding in some cases in the state. It has been observed that hill cattle in the state mostly reared as dual purpose animal i.e milk and draft. Therefore, the hill cattle could be improved for both milk and draft. The aim of the present study is to know the status, characterstics and performance  and to suggest the possible options for improvement in milk productivity of hill cattle in the state.

Method of Maximum Likelihood Estimation (M.L.E.)
    
Method of Moments
    
Method of Minimum X²
    
Interval Estimation