eChapters

Decision Support Systems convert farm data into recommendations that improve livestock productivity, health and sustainability. This chapter explains data-driven, model-driven and knowledge-based systems and their principal components: data management, analytical models and user interfaces. Technologies such as IoT, artificial intelligence, machine learning and cloud computing support applications in feed optimization, disease prediction, reproductive management, milk production and animal-welfare monitoring. Dairy and poultry examples demonstrate how DSS can reduce operational costs and strengthen farm decisions. Implementation challenges include inadequate data quality, high costs, limited technical expertise and maintenance requirements. Future systems are expected to integrate blockchain, robotics, Edge AI and increasingly autonomous management technologies.

Bioinformatics is a branch of biological science which deals with the study of methods for storing, retrieving and analyzing biological data, such as nucleic acid (DNA/RNA) and protein sequence, structure, function, pathways and genetic interactions. It generates new knowledge that is useful in such fields as drug design and development of new software tools to create that knowledge. Bioinformatics also deals with algorithms, databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, structural biology, software engineering, data mining, image processing, modeling and simulation, discrete mathematics, control and system theory, circuit theory, and statistics.

Rural poultry production constitutes vital component of agricultural economy in India. The organized sector of poultry industry is contributing nearly 70% of the total output and the rest 30% by the unorganized sector. In unorganised sector, poultry are reared in free range extensive system with very little input in the form of grain or farm by-products, birds have to scavenge from limited amount of feed resource. The productive out put of these birds are very low (60-70 eggs per bird per annum; 2.0 kg. and 1.5 kg. body weight per bird in males and females, respectively). Moreover, still more than 50 % population of poultry in India comprises of non-descript / deshi birds.

15.1 Sample Objective Question

1. Which one is related to deposition of Micro-organism?
   1. Madrid Agreement
   2. Berne Convention
   3. Budapest Treaty
   4. Patent Cooperation Treaty.
2. WTO was officially commenced in the year...........
   1. 1970
   2. 2003
   3. 2005
   4. 1995
3. Which one is related to protection of Trademarks
   1. Madrid Agreement
   2. Berne Convention
   3. Budapest treaty
   4. None of these
4. One of the four patent office is...........
   1. Patna
   2. Banglore
   3. Varanasi
   4. Mumbai

This chapter describes various multivariate techniques which are useful in animal breeding. The genetic correlation with multiple response variables is already explained in the chapters on Correlation and Genetic Parameters. The other techniques including MANOVA, Principal Component Analysis, Factor Analysis, Cluster Analysis and Discriminant Analysis are explained.

Multivariate analysis is referred to a set of data with multiple response variables in general and such data is encountered often in animal breeding analysis. The simplest form of multivariate analysis is the genetic correlation where two or more traits are analysed together as response variables with factors affecting them on the right-hand side (RHS) and details of these are already explained in earlier chapters.

This chapter introduces the essential components of laboratory work that form the foundation for veterinary parasitology and molecular biology research. A well-equipped laboratory along with proper safety protocols and correctly prepared reagents, is the key to reliable experimental outcomes and a secure working environment.
The following sections describe the standard operating procedures and laboratory biosafety, standard equipment used in research work, and the fundamental principles of solution preparation. These basic laboratory skills are essential prerequisites for the specialised techniques covered in subsequent chapters on veterinary parasitology, molecular biology and bioinformatics. Understanding and applying these foundational concepts allows researchers to maintain experimental consistency, ensure reproducible results, and conduct investigations safely and efficiently. The methods and principles outlined here will be regularly applied throughout the various techniques detailed in this manual, serving as essential knowledge for all laboratory personnel as well short-term visitors.
1.1 Laboratory Standard Operating Procedures and Safety
Laboratory safety is fundamental to protecting personnel, maintaining sample integrity, and ensuring reliable experimental outcomes. This section outlines essential laboratory conduct, safety protocols for personnel and equipment, waste management, and emergency protocols for standard laboratories carrying out diagnostics as well as research.

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

Fracture healing is a complex process in which damaged bone restores its original architecture through a cascade of molecular and cellular events. The process of fracture healing is unique, in that it does not form a poorly organized replacement matrix, otherwise known as scar tissue, but rather regenerates the original matrix and retains its mechanical properties. Normal fracture and bone repair follows a known sequence of events includes: haematoma formation, inflammation, angiogenesis, osteogenesis, and bone remodeling.

For the repair of bone defects, a tissue engineering approach would be to combine cells capable of osteogenic (i.e. bone-forming) activity with an appropriate scaffolding material to stimulate bone regeneration and repair. An ideal tissue-engineered bone substitute should possess 3 elements: osteoprogenitor cells, osteoinductive factors, and an osteoconductive scaffold, and is considered as a potential substitute for autologous bone transplantation. Tissue engineering approaches have proven very effective in bone regeneration recently, and the successful repair of bone defects has been demonstrated in larger animals like canine, goat and sheep.

Stem cells from adult tissues are attractive materials for cell therapy, gene therapy, and tissue engineering. These cells generally have restricted lineage potential when compared to embryonic stem cells, and this may be advantageous in certain therapeutic applications. Till and McCulloch in 1961 describes the presence of hematopoietic progenitor cells in bone marrow. After this, the concept of MSCs is brought to light by Friedenstein and co-workers who demonstrated the osteogenic potential of BM cells by heterotrophic transplantation.

Camels could be exploited for augmenting the country’s milk production capacity as a supplementary milk resource in India for the benefit of increasing human population. Since milk production potential of camels in India have remained unexploited and considering its therapeutic utility it can fetch good prices. Camels are raised for milk, meat, fibre (wool and hair), transport and other work; their dung is used as fuel. Milk is often the most important camel product and is the staple food of nomads.

The camel is an important species of the Indian fragile desert ecosystem, a proven icon of adaptation with its unique bio-physiological characteristics and has formidable ways of living in harsh situations of arid and semi-arid regions. The proverbial Ship of Desert earned its epithet on account of its indispensability as a mode of transportation and draught ability in desert. However, its utilities are subject to continuous social and economic changes. The camel has also played a significant role in civil law and order, defence and battles from the ancient times till date. Camels formed an important component of the Mauryan Army (C.322-232 BC) and continued through the Mughal period (1200-1700 AD) to the present times.

The main purpose of this lecture note is to provide basic information on patent drafting skills and train applicants for drafting a patent in clear and concise manner.

We will see the components of Complete Specification through the following Case studey (1) of a solar water heater. The figures are provided below for reference and better understanding.

True/False

Q.1.    A hypothesis that sets the fundamental objective of scientific study is called the “Research hypothesis”.
Q.2.     A statistical assumption or statement about the unknown parameters of a population is known as Statistical hypothesis.

True/False

Q.1.    According to Prof. RA Fisher “ANOVA” is the separation of variance assignable to one group of causes from the variance assignable to other group of causes.
Q.2.    The basic purpose of ANOVA technique is to test the equality (homogeneity) of several population means from the same 
experiment.

A Patent is a written document explaining an invention with exclusive right. It is used to protect an inventor/scientist and to prevent other from making profits without permission. It is also used as an authority paper permitting license holders to duplicate the results. A patent application requires a combination of legal documentation and technical papers. This issue makes patent writing a very different writing style.

In India, patent applications can be filed by a patent agent or an individual having some scientific and technical background to understand the invention. However, no one can understand the invention better than the inventors themselves. Document preparation requires healthy discussions between the inventor and agent. For inventors, a good understanding on patent writing style can greatly facilitate the documentation process. Before filing the patent application inventor must be sure about that the subject is eligible for patent protection, the invention has something new, the invention is not obvious and is not easily predictable from general consensus, and the invention has practical application.

Biology is in the middle of a major paradigm shift driven by computing technology. Due to the impact of information technology, biological sciences have been rapidly becoming much more computational and analytical. Rapid progress in research in the field of genetics and related field combined with the tools provided by modern biotechnology has generated massive volumes of genetic and protein sequence data over last two decades. Compilation, storage, analysis for extraction of information and knowledge from this data becomes a challenging task as usual analytical procedures are not directly applicable to these data sets.
 
Bioinformatics has been defined as a means for analysing, comparing, graphically displaying, modeling, storing, systemising, searching, and ultimately distributing biological information, which includes sequences, structures, functions, and phylogeny. Thus, bioinformatics may be defined as a discipline that generates computational tools, databases, and methods to support genomic and post-genomic research. It comprises the study of DNA structure and function, gene and protein expression, protein production, structure and function, genetic regulatory systems, and clinical applications. Bioinformatics applications need knowledge from computer science, mathematics, statistics, medicine, chemistry and biology.

Information Sources for Biotechnology
    
Books
    
General Biotechnology Books
    
Animal Biotechnology Books
    
Review Articles
    
Journals
    
Animal Biotechnology Journals
    
Biotechnology Job Listings
    
The Scientist
    
Patents
    
Requirements for Setting up a Biotechnology Laboratory
    
Essential
    
Beneficial
    
Useful
    
PCR
    
Specifications of a Good PCR Macine
    
Gel Documentation System Electrophoresis
    
Laboratory Safety and Hazards

Natural Gene Flow in Plants

More than 70% of plant species, for example potato, oat, rape-seed, wheat, and kiwifruit have come from spontaneous interspecific or intergeneric hybridization although the extent of natural hybridization differs among the plant genera and families. It creates genetic variation and produce recombinant genotypes that may exhibit heterosis.The stabilization of these hybrids often through polyploidy (autopolyploidy or allopolyploidy) can result in the formation of new biological species (Masterson, 1994), thereby, having impact on both ecological and evolutionary processes (Martinsen et al., 2001). In addition, species genomes are often differentially permeable to introgression if they are under simple genetic control (unlike many genes contribute to hybrid fitness with linkages) and certain portion of the genome can readily incorporate alien alleles. Introgression is a selective process of gene flow that filters deleterious genes and takes only beneficial genes (incorporating the alleles from one taxon into other).

Abstract

As we have entered in the post genomic era where we have successfully completed the full genome sequencing of many important crop species and now the future challenge is to ascertain the functions of all genes present in plant genome.

1. Introduction:- Ensuring Quality and Success in Assisted Reproductive
Technology through Good Laboratory Practices Assisted Reproductive Technology (ART) in animals has become an increasingly vital set of techniques with wide-ranging applications across agriculture, wildlife conservation, and biomedical research. These technologies, encompassing procedures such as in-vitro fertilization (IVF), embryo transfer, and cryopreservation of gametes and embryos, offer solutions to infertility, enable genetic improvement in livestock, and play a crucial role in preserving endangered species. As the application of ART expands throughout the globe, the need for stringent quality control and standardization becomes paramount to ensure the reliability and reproducibility of outcomes. Good Laboratory Practices (GLP) represent a well-established quality system that provides a framework for the organizational processes and conditions under which non-clinical studies are planned, performed, monitored, recorded, reported, and archived. Originally developed to ensure the integrity of safety data for pharmaceuticals and other regulated products, the fundamental principles of GLP are highly relevant to the field of animal ART. This report aims to explore the application of GLP in ART and to thoroughly investigate its contribution to achieving successful outcomes in various ART procedures and across different animal species. By examining the core principles of GLP and their practical implementation in animal ART laboratories, this analysis will highlight the critical role of standardized practices in enhancing the reliability, ethical conduct, and overall success of animal reproductive technologies.
2. Good Laboratory Practices (GLP) in Reference to ART
Good Laboratory Practices are broadly defined as a quality system that governs the organization and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, reported, and archived. This system is designed to ensure the quality and

I. INTRODUCTION

Over the last few decades, protection of intellectual property rights (IPRs) has become a global issue. The existing international intellectual property rights regime has been governed by the Trade- Related Aspects of Intellectual Property Rights (TRIPS) Agreement.1 Inspite of maturity of intellectual property laws in post TRIPS era it still desiderate to established coherence with human rights laws particularly right to health and maintain a distance with the socio-economic dimension of intellectual property protection. On the other hand the international human rights instruments from the very beginning appear to be considerable to make certain links with intellectual property rights.2 The issue that is now debated that the adoption of the TRIPS Agreement provisions have serious impact on the realization of certain basic human rights such as the right to health because of non availability and affordability of much needed medicines for the poorer populations of the world particularly in majority of developing countries. Meanwhile TRIPS flexibilities as confirmed by the Doha Declaration on Public Health were adopted in 2001 by the global community to address medicines as public health issue.3 Keeping in view the above developments the problem of right to health and the relationship between medical patents and access to medicine in the case of the HIV/ AIDS and T.B epidemics is complex challenge in the context of the 2030 Agenda for Sustainable Development Goals in 2015 adopted by Member States of the United Nations in Sep, 2015. One of the aims of the agenda is to ensure healthy lives and promote the well-being of people of ages.4 In such a situation it is high time to revisit the existing intellectual property regime in the broader context to protect everyone who is likely to be negatively affected by intellectual property standards imposed by developed nations. Under this backdrop the present paper wishes to examine the various kinds of potential impacts of intellectual property regime on the realization of access to medicine as part of human right to health as universal entitlements amongst the most disadvantaged and marginalized peoples and communities in the broader context of sustainable development goals.

Introduction
Veterinary embryology is a fundamental branch of veterinary science that focuses on the study of the development of embryos in animals, tracing the journey from the moment of fertilization to the point where a fully developed fetus is formed. This field is not just an academic endeavor but a critical component of veterinary practice, research, and animal husbandry. It offers profound insights into the origins of life, the complex processes that drive the formation of tissues and organs, and the ways in which genetic and environmental factors interact to shape the development of an organism. The field of veterinary embryology is vast and encompasses several stages of development. The process begins with gametogenesis, where the reproductive cells—sperm in males and ova in females—are produced through a process of meiosis. These gametes are haploid, meaning they contain half the number of chromosomes found in somatic cells. The union of these gametes during fertilization restores the diploid number of chromosomes, marking the beginning of a new individual organism. The fertilized egg, or zygote, is the starting point of embryonic development. Following fertilization, the zygote undergoes a series of rapid cell divisions known as cleavage. During cleavage, the single-celled zygote divides without growing, resulting in a cluster of smaller cells called blastomeres. This process continues until the formation of a structure known as the blastocyst, which consists of an inner cell mass that will eventually develop into the embryo, and an outer layer of cells called the trophoblast, which will form the placenta. The blastocyst is a critical stage in development as it is involved in implantation into the uterine wall, a necessary step for establishing a successful pregnancy. The next major phase in embryonic development is gastrulation, a highly orchestrated process that results in the formation of three primary germ layers: the ectoderm, mesoderm, and endoderm. These layers are the foundation for all the tissues and organs in the body. The ectoderm gives rise to the nervous system, skin, and sensory organs; the mesoderm