eChapters

A DNA microarray is a multiplex technology used in molecular biology. It consists of an arrayed series of thousands of microscopic spots of DNA oligonucleotides, called features, each containing picomoles (10-12 moles) of a specific DNA sequence, known as probes (or reporters).

Introduction
DNA barcoding is a molecular technique that uses a short section of DNA (genetic marker) extracted from a standardized region of tissue to identify it as belonging to a particular species. A desirable locus for DNA barcoding should be standardized and that should present in most of the taxa of interest and sequencable without species-specific PCR primers. A large database of sequences for the desirable locus needs to be developed. The desirable locus should be short enough to be easily sequenced with current technology, and provide a large variation between species yet a relatively small amount of variation within a species. Although several loci have been suggested, but the mitochondrial Cox1 became a gene of choice for animals and many other eukaryotes. However, the DNA regions that are being used are chosen to optimize the resolution for their specific experimental purposes.

8.1 Sub Cloning
    
8.2 Cloning into a Yeast Artificial Chromosome (YAC)
    
8.3 DNA Libraries

5.1 Replication
    DNA Replication in Bacteria
    DNA Replication in Eukaryotes
    
5.2 Transcription

5.3 Translation

4.1 DNA: The word means
    
4.2 Double Helical Structure: The Watson and Crick Model
    
4.3 Family of DNA 
    
4.4 The Chromosomes

DNA (deoxyribonucleic acid), the giant molecule that carries genetic information in living things, is made up of just a few chemical building blocks that bond together in very particular ways. A typical molecule of DNA consists of two strands that are linked together. A segment can be visualized as a ladder-like structure:

The DNA as made of two strands, a left and a right, which are linked in the middle. In reality, since the ladder is twisted, there really is no “right” or “left,” but we use that terminology here since it applies to the model graphs. The square shapes and diamonds you see in the middles of the “rungs” are the links, which are meant to represent the different chemical bonds between the two strands of DNA.

The selection of right dog for the required purposes like watching, hunting, guarding, showing, companionship and even animal associated therapies nowadays, warrants a detailed information on various dog breeds, like physical and other specific characteristics which may be different to each other. This  chapter  gives important characteristics of dog breeds and guidelines for buying a pup.

Drugs acting on alimentary system

Drugs acting on respiratory system

Drugs acting on urinary system

Drugs acting on genital system

Drugs acting on musculoskeletal system

Antineoplastic drugs

Antimicrobials

Antifungal

Antiprotozoal

Anthelmintics

The drug action can be adversely affected by the presence/concomitant administration of another drug/ agent, may be inhibiting its absorption, metabolism or excretion. Some of the drugs are incompatible with IV fluids, resulting in their inefficacy. The important drug interactions to be taken into consideration while administering a drug, are tabulated in this chapter.

1.     Maintaining Embryonic Stem (ES) Cells
    
2.     Establishing ES Cells
    
3.     Freezing of ES Cells
    
4.     Thawing of Frozen ES Cells
    
5.     Production of Transgenic using ES Cells
    
6.     MTT Assay for Cell Viability

Non-specific uterine infections reduce the reproductive efficiency of livestock.  They are called nonspecific because the initial colonizing bacterium is not known and the specific bacteria causing the signs of infection are not known.  Even though numerous bacteria, in a variety of combinations, have been isolated from infected uteri, Corynebacterium pyogenes and Escherichia coli are usually associated with uterine infections in cattle and sheep.  Empirical therapeutic approach, prescriptions without scientific validation (extra-label use) or inadequate clinical judgment itself to ascertain the nature of uterine infection, obviously due to lack of diagnostic tools are the prime reasons for therapeutic failure for the unsuccessful treatment of uterine infection by large animal practitioners.

1.     Asssay for Catalase
    
2.     Assay for Peroxidase
    
3.     Assay for Poly Phenol Oxidase
    
4.     Assay for Phosphatases

1.     Extraction of b-galactosidase from Yeast Cells
    
2.     Measurement of Enzyme Activity
    
3.     Immobilization of Enzymes
    
4.     Precipitation of Enzyme and Proteins
    
5.     Protien Purification by Dialysis

9.1 Methylases
    
9.2 Polymerases
    
9.3 Nucleases
    
9.4 Ligases
    
9.5 Phosphatase
    
9.6 Topoisomerase
    
9.7 Restriction enzymes

Enzymes are biocatalysts composed of amino acids. Enzyme reactions are highly specific. It binds to the substrate to form a reversible intermediate product called enzyme substrate complex. As the concentration of the substrate increases the rate of formation of the intermediate complex also increases. This increase in velocity of the reaction is in a hyperbolic manner till it achieves the maximum velocity called Vmax .At this point all the enzymes will be in the form of ES complex. Substrate concentration will be in at its optimum level i.e. further increase in substrate concentration will not affect the rate of the reaction. The substrate concentration at which the rate of the reaction is half the maximum velocity Vmax is called the Michaelis-Menten constant, Km. This is constant for the given enzyme acting on the same substrate.

Epigenetics is the study of reversible heritable changes in gene function that occur without a change in the sequence of nuclear DNA and Gene-regulatory information that is not expressed in DNA sequences is transmitted from one generation (of cells or organisms) to the next. Epigenetics has become an integral part of the study of evolution and development during the past 50 years. It was originally defined by Conrad Hal Waddington in 1942 (Waddington, 1942), but it is currently referred to any mechanism that affects genes without changing the nucleotide sequence. These mechanisms are involved in deploying the genetic program for the many processes operating during the lifespan of a cell: development, differentiation, stress response, and pathological conditions, and by the environment to adapt to those situations (Rakyan and Beck, 2006). Epigenetic mechanisms include covalent chemical modification of DNA (methylation) and chromatin (covalent histone modifications), non-coding RNAs, and polycomb group (PcG) genes, and are ultimately related to the regulation of gene expression and chromatin structure.

1.     Equipments

Preliminaries
    
Minimum Variance Unbiased Estimator and Cramer, Rao Inequality
    
Minimum Variance Unbiased Estimator (M.V.U.E.) and Black Wellisation
    
Results on UMVUE and MVUE
    
Efficiency and Consistency
    
Consistency
    
Sufficiency and Completeness
    
Distribution Possessing Sufficient Statistics
    
Sufficient Statistic for Several Parameters
    
Completeness of a Sufficient Statistic
    
Some Important Results in Theorem Forms
    
Minimal Sufficient Statistic
    
Results on Complete Sufficient Statistic

Estrous cycle

After puberty the female enters a period of reproductive cyclicity, which continues throughout most of her productive life.  Estrous cycle is the rhythmic sexual behavioral pattern exhibited by the female, which begins at one estrus (heat) and ends at subsequent estrus.   It is the period between two estruses of the regular cycle.  The reproductive activity of the female is maintained by the co- ordinated functioning of different organs of reproduction (Table: 1.1). Estrous cycle provides repeated opportunities for the females to become pregnant. When conception does occur, the female enters a period of anestrous during pregnancy, which ends after parturition (giving birth) and uterine involution (repair and returning to normal size).

Faulty estrus detection is the most important problem in reproductive management.  There are two requirements for good estrus detection: a skilled observer must spend sufficient time to make observations and cows must show overt signs of estrus during the period of observation.  There are various ways to detect the estrus signs in cows and buffaloes as detailed below.

1.    Palpation of reproductive organs:  Routine rectal examination of all cows between 30-40 days after calving and individual cow that has not been inseminated within two months after calving should be encouraged.   This is to confirm that the reproductive tract is normal and to predict when the next estrus will occur or to identify cows for prostaglandin treatment when estrous cycles are occurring, but estrus has not been detected. 

2.     Teaser bulls:  Teaser bulls are useful in identifying estrus in large number of animals especially buffalo cows.   Teaser bulls can be prepared by vasectomy or by applying apron to a bull. 

3.     Heat detector animals:  Sexually active animals like bulls, cystic cows, hormone treated steer, cows and heifers can be used to identify estrus cows.    They may be fitted with halters containing ink- filled reservoirs and ball paint pen type devices (chin ball markers) that will mark animals that mounted or they can be used without these devices to increase the sexually activity and make visual detection programs more effective.