eChapters

1.     Animal Biotechnology Laboratory
    
2.    Growing Cells with Serum-Basal Medium Eagle (BME), MEM
    
3.     Media Preparation and Sterilization
    
4.     Contaminations in Animal Tissue Culture

India has rich diversity of goat genetic resources and as per 20^th livestock census (2019), there are 148.88 million goats in India. They are mostly found in arid and semi-arid regions of the country and one of the mainstay of dryland agriculture system. Out of 148.88 million, around half of them (75 to 60 million goats) are found in Rajasthan (20.84 m), Uttar Pradesh (14.48 m), Madhya Pradesh (11.06 m), Maharashtra (10.6 m), Tamil Nadu (9.89 m) Karnataka (6.17 m) and Gujarat (4.66 m). Out of total goat population, only 35 % of the goats are of either pure breed or upgraded nature resembling the some of the local breeds. There is huge scope to improve the productivity through use of assisted reproductive tools like artificial insemination and estrus synchronization in field condition to upgrade the non-descript population in relatively shorter period of time as compared to natural services.

Introduction

Greenhouse gases (GHGs) are the atmospheric trace gases which absorb and emit infra-red radiation and thereby trapping the heat radiated from the earth surface. The commonly known agriculturally important GHGs are carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O). The major anthropogenic sources of CO₂ emission in atmosphere are biosphere, burning of fossil fuels and land use changes in tropical forests. The atmospheric concentration of CO₂ has increased from 280 parts per million by volume (ppmv) in 1750 to 394 ppmv in 2012 and is currently increasing at the rate of 1.9 ppmv year-1 (NOAA, 2012). Atmospheric CH₄ concentration has increased from about 715 to 1826 parts per billion by volume (ppbv) in 2012 over the same period and is increasing at the rate of 7 ppbv year-1 (IPCC, 2007; EPA, 2013). Similarly, the atmospheric concentration of N₂O has increased from about 270 ppbv in 1750 to 325 ppbv and is increasing at the rate of 0.8 ppbv year-1 (IPCC, 2007; EPA, 2013).

1    Normal haemotological values

2    Blood biochemical values

The various steps involved in frozen semen technology are vital as the quality of semen determines the conception rate.  The key factor enabling the successful long term storage of frozen spermatozoa is maintenance at ultra low temperature.  Effective semen storage depends heavily on good identification and stock control.  Frozen semen can be easily damaged in the process of repeated handling to establish identity.

Occasionally some diseases can be noticed in male animals, which are responsible for the reduced reproductive performance of the bucks. Sometimes these diseases can damage or obstruct the male reproductive passage and responsible for the infertility and sterility in the bucks. This type of infertility may be associated with pathology of testes, epididymis, vas deferens, accessory sex glands, and urethra or may be associated with abnormal semen production due to congenital or hereditary causes or due to acquired causes.

Heat stress induced by climate change is a serious issue of livestock industries worldwide (Bajagai, 2011). When environmental temperatures move out of the thermo-neutral zone (or comfort zone) animals begins to experience either heat stress or cold stress. Both these stresses require the animals to increase the amount of energy used to maintain the body temperature so that less energy is available for productive processes. Atmospheric temperature, relative humidity, air movement and solar radiation are the major factors that determine the heat load on animal. Thermoregulation is the ability of the animals to maintain their body temperature in cold or hot environments. It consists of behavioural, physiological and anatomical responses that affect energy metabolism. At lower temperature as a physiological adjustment to maintain the body temperature the animal increases the metabolic heat production by increasing the basal metabolic rate and uncoupling of oxidative phosphorylation. Hence the maintenance energy requirement of animals increases in a cold environment, which reduces the amount of energy available for production. From a practical point of view higher temperatures are much more dangerous for producing animals than a cold environment. Temperatures exceeding the higher critical level compromise animal performance not only by changing the energy and nutrient metabolism, but also by upsetting the body homeostasis, with detrimental consequences both for immune competence and for product quality. In general, livestock with high production potential are at greatest risk of heat stress, and require most attention.

Introduction
Genetic research has been transformed by technological developments, and has by necessity become extremely quantitative, as massive quantities of varying complex data types can now be generated very rapidly. High-throughput data are being used in a variety of basic science investigations that have implications in the diagnosis and treatment of human disease: identification and characterization of genetic variants associated with a particular disease within and across populations; discovery of gene expression signatures associated with disease phenotypes; identification and testing of potential disease biomarkers. Methodological advances in the statistical treatment and interpretation of these data are needed in order to meet the pressing need for powerful, efficient and robust analysis.

Some of the Homeopathic remedies followed for common disease conditions of animals are listed in brief in the following chapter.

Evidence over the past few decades has conclusively established that significant changes in climate are taking place worldwide as a result of enhanced anthropogenic activities. The fast pace of development and industrialization and indiscriminate destruction of natural environment, more so in the last century, have altered the concentration of atmospheric gases that lead to global warming. The major cause for climate change and global warming has been ascribed to the increased levels of greenhouse gases (GHGs) like carbon dioxide (CO₂), methane (CH₄), nitrous oxide (N₂O), chlorofluorocarbons (CFCs) etc. beyond their natural levels due to the uncontrolled activities such as burning of fossil fuels, increased use of refrigerants, and enhanced agricultural related practices.

The arid and semi-arid regions of India are drought prone and the availability vegetation of these regions is more acute during summer when the quality of the pastures becomes vulnerable. Small ruminants in the semi-arid regions need to adopt special physiological function to maintain thermal equilibrium. The reproductive efficiency of different breeds of sheep inhabiting in the semi-arid regions of India is relatively low (Arora and Garg, 1998).The nature of nutritional resources and management problems differs with climate, soil and vegetation, the physical process which governs the animals reproductive performance are the same; the female must reach puberty, show behavioral estrus and shed one or more ova which have then to be fertilized. Dietary nutrition promotes the programming and expression of the metabolic pathways that enable animals to achieve their genetic potential for reproduction. Nutrition has an important impact on the reproductive performance in sheep, but the magnitude of the effect on reproduction may vary with the season (White et al., 1983). Sheep are more prone to neglect compared to cattle and often suffer from lack of feed and fodder. The animals during the grazing are exposed to combined stress (Sejian et al., 2010 ab, and 2011) .This leads to decrease in body weight and loss of reproductive functions. Concurrently, Rhind and Mc Neilly (1986) showed that ewes with low body fat reserves had fewer large ovarian follicles and therefore the potential for subsequent maturation and ovulation declined. 

Increasing evidence over the past few decades indicate that significant changes in climate are taking place worldwide as a result of enhanced human activities. The inventions that were discovered during last few centuries, more so in the last century has altered the concentration of atmospheric constituents that lead to global warming. The major cause to climate change has been ascribed to the increased levels of greenhouse gases like carbon dioxide (CO₂), methane (CH₄), nitrous oxides (NO₂), chlorofluorocarbons (CFCs) beyond their natural levels due to the uncontrolled activities such as burning of fossil fuels, increased use of refrigerants, and enhanced agricultural related practices. The temperature increase is widespread over the globe and is greater at higher northern latitudes. Land regions have warmed faster than the oceans. January 2000 to December 2009 was the warmest decade on recordreported by NASA. These activities accelerated the processes of climate change and increased the average global temperatures by about 0.8°C (1.5°F) since 1880 (NASA,2010). Global average sea level has risen since 1961 at an average rate of 1.8 [1.3 to 2.3] mm/yr and since 1993 at 3.1 [2.4 to 3.8] mm/yr, with contributions from thermal expansion, melting glaciers and ice caps, and the polar ice sheets. Satellite data since 1978 show that annual average Arctic sea ice extent has shrunk by 2.7 % [2.1 to 3.3%] per decade, with larger decreases in summer of 7.4% [5.0 to 9.8%] per decade. Mountain glaciers and snow cover on average have declined in both hemispheres. It has also induced increased climatic variability and occurrence of extreme weather events in many parts of the world.

Yak (Poephagus grunniens)is the most remarkable and multipurpose domestic animal living at high altitudes of the Himalayas. Features common to the environment in which yak live are extreme cold, mountainous terrain, high altitudes with reduced oxygen in the air, high solar radiation and short growing seasons for herbageanda variable assortment of herbage, sparse insome areas. Generally, the yak habitat ranges from 3000-6000 m abovemean sea level (msl), where the alpine pastures are found and there is no absolutely frost-free period during any part of the year. Wilted herbage provides some sustenance for the yak at other times of year, but not insufficient quantity for their requirements (Fig. 9.1). Many of thecharacteristics of the yak can be regarded as adaptations to these conditions, in which cattle of other species have difficulty in surviving (Wiener et al., 2003).The yaks are considered as the life line of the highlanders in remote terrain because yak is the only sustainable livelihood due to non-availability of arable land for major agriculture. Yak husbandry is one of the important and indispensable aspects of the Indianstates, namely, Arunachal Pradesh, Himachal Pradesh, Jammu and Kashmir, Sikkim and Garhwal hills of Uttarakhand where other livestock species can hardly live but yaks can survive, reproduce and produce milk, meat, wool and other by-products.

Veterinarians will be often required to mention the specific animal husbandry terms for various purposes like writing certificates for various schemes, postmortem, attending court of law in veterolegal cases etc. Hence this chapter aims in refreshing the vets with the common terms associated with different domestic animals and animal husbandry activities. 

Partialy Balance Incomplete Block Design (PBIBD)
    
Interblock and Intrablock Information

Buffalo plays an important role in Indian livestock economics.  India has more than the half of the world buffalo population, contributing 50% of total milk production of the country.  The buffalo is said more productive than an average cow due to the higher fat percentage (7%) in the milk, better feed conversion ability and disease resistance.  Buffaloes have lower reproductive efficiency in the form of delayed age of maturity, longer calving interval, weak / silent estrus, seasonality of breeding, poor libido and poor semen quality. A large number of buffaloes do not conceive due to some gynaecological problems and are being slaughtered every year.  The most commonly observed reproductive disorders are detailed below.

Information Sources for Biotechnology
    
Books
    
General Biotechnology Books
    
Animal Biotechnology Books
    
Review Articles
    
Journals
    
Animal Biotechnology Journals
    
Biotechnology Job Listings
    
The Scientist
    
Patents
    
Requirements for Setting up a Biotechnology Laboratory
    
Essential
    
Beneficial
    
Useful
    
PCR
    
Specifications of a Good PCR Macine
    
Gel Documentation System Electrophoresis
    
Laboratory Safety and Hazards

Biotechnology Products and Technologies
    
Bottlenecks in Fast Progress of Animal Biotechnology
    
Why Biotechnology should be Used in Animal Husbandry and Dairy Production
    
Contribution of Livestock Sector to Food Basket
    
Biotechnology and Environment
    
Biotechnology of Nutrient Partitioning
    
Genetic Programming for Better Animals
    
Biotechnology of Reproduction
    
Biotechnology of Animal Health

1.1    General Introduction, History, Ownership of Tangible and Intellectual Property
    
1.2    Patentable Subject Matter
    
1.3    Basic Requirements of Patentability
    
1.4    Novelty, Public Domain, Obviousness and Non Obviousness
    
1.5    Inventive Step (Non-Obviousness) and Industrial Application

Basic thought of Sampling

The data is very important in every field for decision making whether it is medical sciences, engineering sciences, agricultural sciences and management sciences etc. for this we need to plan the correct data. If we don’t have correct data then based on that we cannot plan good decisions and it will provide misleading results. So, to achieve or plan the right data it is necessary to have the right technique which is to be used while collecting data and an appropriate method of data collection is preferred. From this, the two important questions arise (i) what are those methods required to obtain paramount data or sample and (ii) how best to use the data or sample to estimate the characteristic of the whole population i.e., what are those methods by which we can study our population parameters to draw a proper statistical inference.