eChapters

Evolution is a self-occurring natural phenomenon by which new organisms develop gradually as a result of change in genetic pool of a population. It is a very widely and historically studied phenomenon which still surprises scientists due to its underlying complexity. The three processes which contribute most to evolution are genetic drift (also known as random drift or allelic drift), natural selection and mutations. Genetic drift and natural selection act at a population level, whereas mutations act at individual level. For evolution to occur, the changes these processes bring about must be heritable, which they are.
 
Genes are present on chromosomes inside nucleus of the cell and the collection of all the genes in a cell is known as the genome of that cell. Genome size and complexity varies from species to species. Simple organisms, such as bacteria and protozoans have a very simple genome, with very few genes, whereas complex higher organisms such as humans have a large genome, with thousands of genes. The position of a gene on a chromosome is known as a locus. Organisms may have more than one copy of a chromosome in the cell.

Q.1.    What is the most fundamental assumption to be followed by random variable for using the standard method of parametric statistics or parametric inference ?

    Answer
    
    The most fundamental assumption required to be followed by random varibale for using the parametric statistics to have statistical inference drawn from certain set of data is that the random variables have normal distribution.

Non-Probability sampling methods are also known as non-random sampling methods or deliberate sampling. These types of methods are reliant on the researcher’s ability to select members at random. These sampling methods are not fixed or pre-defined selection processes making it difficult for all elements of the population to have equal opportunities to be included in the sample.

Types of non-probability sampling techniques

1. Judgmental or Purposive sampling

This technique involves the purposive selection of units of samples based on the judgement of the researcher. The researcher selects the sample on the basis of his a priori knowledge and experience.

Non-Response

Non response occurs when respondents are not available or do not occur in the survey and refuse to respond by any means. This non response is mainly of two types either item non response or unit non response.

If we are conducting an online survey and mailing a questionnaire to various persons. But sometimes we get a reply for some questions and sometimes not i.e., in case of questions in which income is concerned. In some cases, the mail is ignored due to lack of time. So, if the whole questionnaire is ignored by any means, then it is known as non-response and if some of the questions are ignored due to their fussy nature such type of response is known as a unit non response. Such types of non-response problems are very common in surveys. Whenever we carry out a survey non response always exists because it is almost impossible to get every response from each individual.

The individual chosen for the sample is not ready to participate in the survey is known as non-response. This type of selection bias is unit/item non response.

The basic knowledge of normal physiological values in different animal species is essential for selection of the animal, diagnosis of any abnormality if any and its correction. The chapter provides the normal physiological values viz temperature, pulse, respiration etc in different domestic animals.

Introduction

Until the 1960’s poultry in India remained largely a backyard dual purpose activity for eggs and meat. People from low social order and economically weaker sections in villages and suburbs generally took to poultry keeping primarily to supplement their diet with more nutritious eggs and meet, and secondly to enrich their livelihood. Despite on a small scale, the activity attracted sizeable proportion of population contributing immensely to the egg and meat supply. The Indian society, though largely vegetarian, gradually accepted eggs and meat in their diet. As demand for poultry products increased, small units transformed into larger well organized farms paving way for a poultry products supply network throughout the country.

One Health is defined as “the collaborative effort of multiple disciplines working locally, nationally, and globally to attain optimal health for people, animals and the environment.” The concept of ‘One Health’ dates back to the Greek physician Hippocrates (ca. 460 BCE–a.370) who in his text “On Airs, Waters and Places” stated:

Whoever wishes to investigate medicine properly, should proceed thus: in the first place to consider the seasons of the year, and what effects each of them produces, for they are not all alike, but differ much from themselves in regard to their changes.

6.1    Copyrights
    
6.2    Trademarks
    
6.3    Geographical Indications
    
6.4    Trade Secrets

Introduction
At the beginning of the 21^st century, with a population of 6.1 billion in 2000 and expected to be headed for 9.1 billion by 2050 (UN News Centre, 2005), the challenge of yet again increase food production by many folds only in 50 years has become a daunting task in itself. The situation is further worsen because now, we need to increase food production in sustainable way by 2050 on approximately the same area of arable land using less resources, particularly, fossil fuel, water and nitrogen, at a time when we must also mitigate some of the enormous challenges associated with climate change. Furthermore, there is the critical and urgent humanitarian need to alleviate poverty, hunger and malnutrition which is afflicting more than 1 billion people

4.1    Provisional and Complete Specifications
    
4.2    Publication
    
4.3    Request of Examination
    
4.4    PCT Application: Gateway to Foreign Patents

5.1    TRIPS
    
5.2    WTO
    
5.3    WIPO
    
5.4    Paris Convention
    
5.5    Berne Convention
    
5.6    Budapest Treaty
    
5.7    Madrid Agreement Marks (MAM)
    
5.8    The Patent Law Treaty (PLT)

2.1    Specification Drafting
    
2.2    Methods and Precautions in Drafting Patent Specification
    
2.3    Disclosure Requirements
    
2.4    Patent Claims
    
2.5    Filing of A Patent Application

Introduction

Microbes (bacteria, archea and eukarya) are single celled organisms that rule the planet. Microbes occupy every possible niche, from the high atmosphere to the deep ocean, from hot springs to artic ice, from hot deserts to deep forests including many regions that cannot yet be accessed for study. The biosphere contains between 1030 and 1031 microbial genomes, which is about 2-3 times more than the number of plant and animal cells combined (67). In fact, studies have shown that microbes associated with the human body are about ten times more than the number of human cells (5).  Microbes play a critical role in the life cycle of all living organism and environment in general.  Some microbes cause disease, but the most of them are either innocuous or play a role in physiology and cellular response including immune response, digestion and vitamin production of various organisms.

The Fourth Assessment Report of the United Nations Intergovernmental Panel on Climate Change (IPCC), after analysis of more than 6000 peer reviewed publications has assessed potential effects of changing climate on various spheres of life (Solomon et al., 2007). Under different scenarios, the estimated temperature rise of 1.1 to 6.4 °C in the global surface temperature is predicted at the end of 21st century (period 2090-2099 relative to 1980-1999). Global average sea level in the last interglacial period (about 125,000 years ago) was likely 4 to 6 m higher than during the 20th century, mainly due to the melting of ice in the polar regions. It has been detected in changes of surface and atmospheric temperatures in the upper several hundred metres of the ocean, and in contributions to sea level rise (Solomon et al., 2007). The change in climate is concurrent with the changes in global levels of carbondioxide, methane, oxides of nitrogen etc, loss of biodiversity, natural habitat and other natural resources. The IPCC report also estimates a confidence level of 90% that there will be more frequent warm spells, heat waves and heavy rainfall and a confidence level of 66% that there will be an increase in drought, tropical cyclones and extreme high tides. The magnitude of the events will vary depending on the geographic zones of the World (Solomon et al., 2007).

• In male animals, endocrine regulation of reproduction is mainly controlled by pituitary gonadotrophins and gonadal androgens. It is not same as in females as there is only negative feedback controls in case of male while it is positively as well as negatively feedback control in females. In other words the synchrony of pituitary gonadotrophins with gonads in male is non cyclic while in female it is cyclic i.e. after castration the pulse frequency of LH and FSH is retained
• Sex Hormones are sex specific e.g. estrogen and progesterone works primarily in females and testosterone in males but sex specificity is limited as far as sexual behaviour is concern. As estrogen injection in castrated buck recovers the maleness while testosterone injection in ovariectomized female maintain the receptivity for male up to certain extent.
• Hypothalamus acts as interface between nervous system and endocrine system. GnRH synthesized in neurons in hypothalamus, released at nerve endings episodic manner and transferred via hypophyseal portal system to anterior pituitary to stimulate the secretion of gonadotrophins.

Abstract

DNA barcoding, an additional global standard taxonomic tool for identifying plant species helps the non-taxonomists also to identify the plant species in a more reliable way with the help of devices including nanotechnology. Researchers proposed various genes or gene portions as potential barcodes either singly or in combination, yet there is no standard/ universal barcode DNA region for plants. The more accurate plant DNA barcode region would have to be from highly variable markers in the nuclear genome preferably from multiple loci and in combination with variable DNA regions of chloroplast genome, to resolve the problems that arise due to hybridization, allopolyploidization and introgression. Nonetheless, the phylogenetically informative sites might not be the potential DNA barcode regions as the former requires synapomorphies unlike autapomorphies with high levels of informative characters in the latter DNA taxonomy, but could provide insights into genetic distances between species.

1.     Isolation of Plant DNA
    
2.     RAPD Analysis of Genomic DNA
    
3.    Agro Bacterium based Transformation in Plants
    
4.     Assay for Gus Operon
    
5.     Confirmation of Transformation using Polymerized Chain Reaction (PCR)
    
6.     Primer Designing
    
7.     Isolation, Purification and Identification of Plant Secondary Metabolites
    
8.     Evaluation of Antioxidant Activity of Plant Extract
    
9.     Estimation of Total Phenolic Content in A Plant Extract
    
10.     Estimation of Total Flavanoid Content

1.     Plant Tissue Culture Laboratory
    
2.     Cleaning and Sterilization of Glass Wares
    
3.     Media Sterilization
    
4.     Media Composition and Media Preparation
    
5.     Callus Culture in Carrot
    
6.     Culture of Shoot Tip (Vanilla)
    
7.     Anther Culture of Datura
    
8.     Subculture of Carrot Callus
    
9.     Hardening of Plantlets
    
10.     Isolation of Protoplast from Plant Leaves

1    General post mortem examination and detection of date and time of death

2    Pathogenomonic lesions of different diseases

3    Livestock insurance

4    Pet-dog insurance

Introduction

India is an agriculture based country with 60% of the manpower employed in agriculture (World Bank, 2010). Few of the main environmental issues are soil erosion, water pollution from raw sewage and runoff of agricultural pesticides. There is a need to maintain a sustainable environment and a sustainable agricultural system.  Sustainable Agricultural system is a self sustaining system, is dependent up the ability to bring together and manage simultaneously the soil, water, plant and animal resources with the limitation of climatic and environment in such a way that the agricultural production/profitability can be maintained in the future too. So initiatives need to be taken to achieve a sustainable agricultural system. Tools have been developed to aid agriculturist, extension personnel and policy makers. The World Wide Web has made the tools easy accessible.