eChapters

Systematic Sampling (Meaning and Concept)

In this chapter, we are going to discuss the systematic random sampling technique. This is a random / probability / quantitative sampling method. In our previous chapter, we discussed the stratified random sampling technique. We have said that this technique is used when the population is heterogeneous and needs to be stratified into homogeneous groups. We have also discussed steps to be followed when using stratified random sampling as well as the strengths and limitations of stratified random sampling. Systematic sampling is a commonly employed technique if a complete and up-to-date list of the sampling units is available. The procedure involves choosing the very first object arbitrarily and the remaining members of the sample will be selected naturally according to any pre-decided sequence containing regular spacing of units. In this method, units of the population are numerically, geographically, and alphabetically arranged either in ascending or descending order (generally ascending order is preferred). When the ordering of the elements is related to the characteristic of interest,

r DNA Biotechnology
    
Concept of r DNA
    
DNA Preparation from Tissue
    
Reagents
    
Preparation
    
DNA Buffer (Tris-EDTA)
    
RNase A (20 mg/ml)
    
Toe DNA Preparation for Southern Blots
    
Southern Blot
    
PCR
    
Polymerase Chain Reaction
    
The Cycling Reactions
    
Denaturation at 94°C
    
Annealing at 54°C
    
Extension at 72°C
    
DNA Sequencing
    
The Sequencing Reaction
    
Denaturation at 94°C
    
Annealing at 50°C
    
Extension at 60°C
    
Separation of the Molecules
    
Detection on An Automated Sequencer
    
Assembling of The Sequenced Parts of a Gene
    
Freezing of Buffalo Semen (Jindal, 1995)
    
Tissue Culture
    
Preparation of Media
    
Laboratory Procedure for IVF (Kharche et al., 2008)
    
Source of Oocytes
    
Preparation of Estrous Goat Serum
    
Collection of Oocytes
    
Laparoscopic Ovum Pick-up
    
Oocyte Retrieval from Ovaries Collected form Slaughter 
    
House
    
Slicing Technique
    
Follicle Puncture Technique
    
Follicle Aspiration Technique
    
Follicle Isolation Technique
    
Evaluation of Oocytes
    
In vitro Maturation of Oocytes
    
Preparation of Cells for Co-culture
    
Assessment of Nuclear Maturation After in vitro Maturation of Oocytes
    
Analysis of Nuclear Status is Ascribed to One of the Following
    
Sperm Preparation for in vitro Fertilization of Oocytes
    
In vitro Fertilization of Oocytes
    
Assessment of in vitro Fertilization of Oocytes
    
Assessment of Cleavage
    
In vitro embryo culture

7.1 Gel Electrophoresis
    
7.2 Polymerase Chain Reaction (PCR)
    
7.3 Restriction Fragment Length Polymorphisms (RFLPs)
    
7.4 Random Amplification of Polymorphic DNA (RAPD)
    
7.5 Amplified Restriction Fragment Length Polymorphism (AFLP)

Test for Significance for Population Mean
    
Test for Significance of Population Proportion

2.1     Regulation of the Cell Cycle 
    
2.2     Mitosis
    
2.3     Meiosis

1.1     Prokaryotic 
    
1.2     Eukaryotic
    
1.3     Cell orgenellie

Abstract
This paper presents a lucid and application-oriented delineation of the theory of probability which has proved itself as a basic tool in the theoretical and applied researches in almost all disciplines of science, specially, in the problems encompassing the real-life issues. Bio-informatics has recently emerged as a potential discipline of science and, needless to mention that the application of the probability theory has delved deep to elucidate the laws under its ambit. The inference procedures based on biochemical systems require the formulation of stochastic models (owing to the prevalence of low to moderate levels of stochasticity and noise in their dynamics) which, in turn, are developed using the probability laws in abundance. In Systems Biology, the estimation of parameters poses a prime task in biokinetic models developed on experimental data, and such estimation procedures have been possible only by applying appropriate tools derived using probability. Likelihood-free Bayesian inference that explicitly accounts for discrepancies between the model and the data, termed Approximate Bayesian Computation under model uncertainty is an off-shoot of the celebrated, Bayes' theorem of probability.

Preliminaries and Test Based on N. P. Lemma
    
More on Testing of Hypothesis

Infertility means reduced fertility or the cow is ultimately capable of becoming pregnant and giving birth to a live calf but the interval may be much longer than 12 months. 

I. Problems leading to infertility

1. No signs of estrus

When the animal is pregnant and for a short time after calving the cows may not show estrus signs. A thorough palpation of the ovaries and tubular genital tract should be performed and a repeat examination may be necessary.

The camel is an important animal component of the fragile desert eco-system. With its unique bio-physiological characteristics, the camel has become an icon of adaptation to challenging ways of living in arid and semi-arid regions. The proverbial Ship of Desert earned its epithet on account of its indispensability as a mode of transportation and draught power in desert but the utilities are many and are subject to continuous social and economic changes. The camel has played a significant role in civil law and order, defence and battles from the ancient times till date. The world famous Ganga-Risala of erstwhile Bikaner State was accepted as Imperial Service Troup and participated in World War I and II.

The ultrasonography is a technique which uses sound waves of very high frequency to scan the various organs of the body. The ultrasound waves of 2-10 mHz frequency are used in veterinary practice. A quartz crystal when stimulated by short pulse current ultrasound waves are emitted. The ultrasound waves emitted are directed towards a specific target organ are reflected back as echoes. The echoes are received by the same quartz crystal and are processed in the electronic circuit and finally displayed as image on the monitor.

10.1 Plasmids
    
10.2 Retrovirus

The UN designation of 2013 as the International Year of Water Cooperation aptly demonstrates growing gap between water supply and demand. These symptoms, which are already visible in a few regions around India, are soon to assume a national proportion and may become permanent feature of the water sector in the country, unless suitable policies are adopted quickly to manage water demand and supply at different levels. Water demand is growing fast due to a rapid population growth and economic activity, but water supply is not growing at the same rate because of serious ûnancial and physical limits for supply augmentation. Water resources developed at present amount to about 680 km³, which constitutes 61% of the utilizable potential of 1122 km³. However, it is difficult to add supply beyond this level due to heavy costs, environmental concerns and interstate water conûicts. In contrast, the total demand for water is projected to reach 784–843 km3 by 2025 and 973–1180 km³ by 2050 (Ministry of Water Resources, 2000). Recent research studies predict that, if the demand– supply gap continues to increase, nine basins that have over four-ûfths of the total water use in India will face physical water scarcity by 2050 (see Amarasinghe et al., 2007). For a heavily populated, monsoon-dependent and rural-based country, such as India, water scarcity of this magnitude will not only lead to serious water conûicts among sectors and regions, but also have a devastating effect on the food and livelihood fronts.

India has shown to the world that with 2.4 % of the land and 4 % of the water resources available at the global level, it can provide the food security to its millions. A record production of almost 265 million tonnes in 2013-14 is a testimony to the strides taken in the field of agriculture. However, the agriculture sector faces many new challenges in the future and climate change (global warming) is perhaps the most formidable one.

The new emerging demands of the relatively more-affluent Indian population, particularly its middle class, coupled with a net cultivated area unlikely to exceed 143 Mha in 2050 as well as an estimated rainfed agriculture to cover around 45 % of the net sown area, are further compounded with the harsh reality that highly productive agricultural land is being continuously lost out to the industry and urban sectors. How will the country meet the target of 400 Mt of food grain production is the mute question, in a situation where natural resource base is continuously degrading and climate change with its attendant impact is adversely affecting the agricultural production system (Singh, 2014).

Introduction

In many developing countries including India, agricultural sector is of vital importance to the economy and welfare of the inhabitants. Agriculture contributes to 14% of the Indian GDP and supports the livelihood of two-thirds of the population. Despite tremendous technological developments, vagaries of weather continue to plague the agriculture production. Any shortfall in agricultural production will have its cascading effect on the economy of the country. In recent times, climate change and variability are confounding the troubles already faced by Indian agriculture and the farming community. Loss in crop production and farm income due to unexpected weather hazards is beyond the carrying capacity of resource poor farmers of the country. With the growing commercialization of agriculture, the magnitude of crop loss due to unfavourable weather hazards is increasing leading to suicides of farmers. The State and National governments on their part are coming to rescue of farming community by implementing various relief measures like reduction or suspension of land revenue taxes, loan waiving, relief from calamity relief fund etc. Though these measures are helpful, farmers cannot expect them as a right and they put lot of burden on the country’s exchequer.

Introduction

Adoption of green revolution technologies during 1960s led to increased productivity and elimination of acute foodgrain shortages in India. These technologies primarily involved growing of high-yielding dwarf varieties of rice and wheat, increased use of chemical fertilizers and other agrochemicals, and spread of irrigation facilities. This was also accompanied by other modern methods of cultivation, which included maximum tilling of land, virtually clean cultivation with complete removal of crop residues and other biomass from the field, fixed crop rotations mostly involving cereals, and elimination of fertility- restoring pulses and oilseed crops in the highly productive north-western plain zone of the country.

Constantly emerging and reemerging vaccine-preventable infectious diseases recently around the globe challenged the health infrastructure and has been a critical concern for the medical fraternity. There are 21 other neglected tropical diseases, as classified and recently updated by the World Health Organisation, many of which are seemingly vaccine-preventable. The unprecedented scenario that the world encountered during the recent COVID-19 pandemic was also a ‘silver lining in the dark cloud’ for primarily the vaccinologists. The scientific world witnessed stupendous developments in vaccine development. One such instance was the cutting-edge mRNA vaccine development platform. Once a vaccine is successfully designed, the next step is scaling it up in a milieu that is conducive for its mass production. There are numerous ways to achieve it, from biological system (animal models like the chick embryo, mouse cell-lines, suspension-cultured tobacco cells) to the non-biological (bioreactor) settings. This chapter provides a comprehensive overview of the intricacies in vaccine development, from designing to large-scale manufacturing (mass production). It delves into key scientific guiding principles behind developing and formulating vaccines, adopting both the traditional and emerging bioprocessing and bioseparation engineering and technology. It also explores the technical and management challenges faced before and during scaling up, including maintaining product efficacy, ensuring high quality for human administration, and navigating regulatory landscapes, among others. The chapter offers a holistic understanding of bioprocessing continuum, to educate and inform the research (academia) and development (bioindustry) community for more promising and effective strategies in the future to optimise vaccine production, thus contributing to the global public healthcare initiatives.

The following plants have been worked out in the laboratory of Prof. N. Raaman: Cynodon dactylon, Artemisia pallens, Leptadenia reticulata, Clausena dentata, Tylophora indica, Aristolochia bracteolata, A. indica, Cissus quadrangularis, Hypericum mysorense. and Solanum trilobatum. Out of some of the data of these plants have been included in the previous chapters. In this chapter, compound isolation (CD1) from Clausena dentata (worked by Dr. R. Kamaraj) and characterization of the compound are given in detail.