eChapters

9.0. Introduction

Enzymes are organic biocatalysts which initiate and control biological reactions. A catalyst is an agent affecting the velocity of a chemical reaction without appearing among the final products of the reaction. Unlike general catalysts, enzymes are specific in nature and function at a temperature of approximately 37^o C and pH close to neutrality. All enzymes are proteins but all proteins are not enzymes. These are distributed among the cell and tissues of plants and animals. Use of malted barley in brewing, yeast in the production of alcoholic beverages and wrapping of meat in the bruised leaves of papaya tree for tenderizing meat are techniques that have been used for centuries. In bread and cheese making also, enzymes have been exploited from generation to generation. Thus, enzymes have been used without knowing the nature of enzyme as catalysts and reactions involved. However, it is only recently that nature and action of enzyme is known and its importance in food industry is realized.

Development of the country build is upon the aspect of the human measures. Imaginative, inventive, truthful and persevering persons head the nation in the virtuous path and guide to all aspects of growth in every field. Entrepreneurs drive nation’s growth. Entrepreneurs are familiar for their specific features Acknowledging this reality on growth of entrepreneurship. Entrepreneurship is an ingenious and imaginative reaction to the surrounding. The expression “entrepreneurs” normally mention to person who tackle recently developed capabilities that are imaginative and inventive. Analysis towards the entrepreneurial character has manifest that majority of the successful entrepreneurs share a defined category of personality features. This acquires the peculiarity as to. The existing analysis is an effort to inform the main features of entrepreneurs in food processing enterprises of Assam.

Introduction

The camel is an important animal part of the desert eco-system. With its unique characteristics, the camel has becomes adapted to tough way of living in arid and semi arid region. The camel called “Ship of desert”, because he can walk across the desert, even on a hot afternoon and Hot sand also can’t affect it. He can run on hot sand@ 25 km per hour with his thick and padded feet, so hot sun does not burn them. In desert, there are no rivers or lakes, then also it can’t affect to camel because he can drink 46 litres of water at a one time. He can eat a lot of food at one time, so he doesn’t need to eat for several days. Camel eats leaves, but there are no trees in a desert, so he can eat thorns with the help of thick lips and thick tongue. Camels in India are reared under extensive type of system. Camels are also kept in small numbers under intensive system, particularly for the routine work such as camel cart traction, transport for men and materials, agriculture operation. Seasonal movement of camel herds to the areas having comparatively better pasture facilities is a quite common practice. A camel is even toed ungulates that having distinctive fatty deposit on its back (hump). Camels have been domesticated as livestock. They provide food (milk + meat) and textile (fibres and felt from hairs). Camels are working animals especially suited to desert habitat as vital means of transport for passengers and goods. There are three species of camels.

The state of Uttaranchal was carved out of Uttar Pradesh on 9^th November 2000 as the 27th state of the union of India and renamed as Uttarakhand. The state is located in western Himalayan region and is one of the geographically smallest states of the country. However, the state is enriched with livestock biodiversity represented by cattle, buffaloes, goats, pigs, horses, mules and poultry.  The climate of the state varies from subtropical in the valleys to temperate on higher slopes with a summer monsoon. The temperature ranges from -5.4ºC (Muketshwar, higher hills) to 40.2ºC (Pantnagar, plains) but it drops below freezing point in many parts of high mountainous areas of the region during winter. Preponderance of hilly terrains and forestland restricts the size of the agricultural land/ holding. The average rainfall recorded in the year 2008 was 1605 mm.  Higher rainfall, abundance of natural pastureland and suitable climatic conditions makes the state favorable for livestock development. The human population was 84.89 lacs with a density of 159 people per sq km during the year, 2001.

Introduction

In India there are about 200 distillery having installed capacity 1555.5 million liters per annum out of which 50% are of 30 KL/ per day capacity about 50% of remaining distillery are of 45 to 60 KL/ per day capacity there are few distilleries which having go 120 KL/ per day capacity. Distillery waste is one of the major industrial waste having high organic and inorganic constituents in the recent past the treatment and disposal of distillery waste has become a subject of debate of each and every forum.

Introduction

Chickpea (Cicer arietinum L. ) popularly known as Gram, Bengal gram, Egyptian pea, Chana, or Kabuli chana, Garbanzo bean is one of the first grain legumes to be domesticated by humans in old world (Van der Maesen, 1972). The genus Cicer belongs to the sub-family Papilionaceae of the family Leguminoceae (Bentham and Hooker, 1970) now popularly known as ‘Fabaceae’ and have chromosome number 2n = 2x = 16. The origin of the crop is considered to be Western Asia from where it has been transferred in India and other parts of the world (Rathore and Sharma, 2003). Importance of pulses is relatively more in our country as its contribution in nutrient supply is far more in Indian diet than that in Asia and world as a whole (Ali,2002).

Cheese is a milk product of ancient origin and portrays the ethos of civilization and its cultural evolution. Cheese is one of the oldest foods of mankind. It is commonly believed that cheese evolved in the Fertile Crescent between the rivers Tigris and Euphrates in Iraq some 8000 years ago. The so-called Agricultural Revolution occurred here with the domestication of plants and animals.

It seems that the cheese originated accidentally as a result of the activities of nomadic tribes. Since animal skin bags were a convenient way of storing liquids for nomadic people, these were used for storing surplus milk. Fermentation of the milk sugars in the warm climate prevailing would cause the milk to curdle in the bags. The swaying animals would have broken up the acid curd during journeys, to produce curds and whey. The whey provided a refreshing drink on hot journeys, while the curds, preserved by the acid of fermentation and a handful of salt, became a source of high protein food supplementing the meager meat supply.

This activity gave rise to the assumption that cheese was evolved from fermented milks. It is perhaps more probable that the crude fermentations progressed in two ways. In one direction towards the production of liquid fermented milks such as dahi, yoghurt, laban, koumis and Kefir and in the other direction through the drainage of whey through a cloth or perforated bowls, to leave solid curds which when salted became cheese.

It was a prominent article of the Greek and Roman diet as much as 2500 years ago. It is referred to in the Old Testament several times.

The term “chelate” comes from the Greek word “chel,” meaning “claw,” indicating that the element is tightly held or clawed by the ligand. The term was coined by Morgan and Drew in 1920, defining it as a chemical compound in the form of a heterocyclic ring with a metal ion attached by coordinate bonds to at least two non-metal ions, known as chelation. A chelate is a type of mineral complex, which is a mixture of a mineral and an organic compound like a protein or polysaccharide. Chelation refers to the bond formed between a metal ion (mineral) and a ligand (protein or amino acid chelating agent). Chelates are created by reacting a mineral salt with an enzymatically prepared mixture of amino acids and small peptides under controlled conditions. The ligand binds the metal at multiple points, forming a ring structure with the mineral, resulting in a chelate.
In animal feed, chelated minerals, such as iron, manganese, cobalt, copper, and zinc, are commonly used to enhance bioavailability and support metabolic functions. These trace elements are transitional elements on the Periodic Table, forming stable complexes or chelates due to their ability to form coordinate covalent bonds.
The primary goal of chelates is to improve mineral absorption in animals during periods of high nutritional demand, such as pregnancy, lactation, weaning, and environmental or disease stress. Chelates have been studied for their effects on immunity, reproductive performance, and overall herd health. To maximize mineral absorption, chelated compounds should be stable in the rumen.
Chelates are stable, electrically neutral complexes that protect trace minerals from chemical reactions during digestion, ensuring their availability to the animal. Chelated minerals are used to increase bioavailability and uptake, reaching the plasma intact and separating at the site of action. They can replace a portion of inorganic minerals in animal diets and are suitable for all species. Soluble chelated trace minerals are designed for use in drinking water supplies.

Preparation of Samples

Before withdrawing portions for analytical determinations, bring the sample to a temperature of 26 to 28ºC and mix throughly by pouring gently into a clean dry receptacle and back, until a homogenous mixture is assured. If lumps of cream do not completely disappear, warm the sample to about 40ºC, mix throughly, then cool to 26 to 28ºC. In case a measured volume is required in a determination, bring the temperature of the sample to about 27ºC before pipetting. All samples shall be allowed to stand for three to four minutes after mixing to allow air bubbles to rise ; the sample bottle shall then be inverted three or four times immediately prior to taking the milk for analysis. If a sample is curdled, excessively churned or decomposed, the sample should not be taken up for analysis.

The chemical composition and digestibility of feedstuffs influence their nutritive value for animal production. Therefore, assessment of nutritive value of feedstuffs continues to be one of the top priority areas in animal production research. Although traditionally the feedstuffs were evaluated by performance studies and digestion trials, such techniques are expensive and requires more time and labour. Continued search for alternatives to animal feeding experiments have led to the development of feed evaluation systems that are rapid and economical.

In general, there are two kinds of laboratory analyses — Chemical analyses and Biological tests with rumen micro-organisms or purified enzymes. Biological tests give direct estimates of digestibility, but are lengthier and more expensive. Chemical analyses are cheaper and rapid, but do not give direct estimates of nutritive value. The statistical association between the chemical components and quality need to be established to predict the nutritive value.

Preparation and processing of samples for chemical analysis: The preparation of samples depends upon the purpose of analysis and the nature of the constituents that are to be determined. The preparation of samples for analysis is as important as the analytical procedure and hence, it should be done with utmost care. (A) Smpling of wet material: (1) Plant material; (2) Silage; (3) Cattle faeces; (4) Sheep / goat faeces; (5) Poultry excreta; (B) Sampling of air – dry material: (I) Hay or straw; (II) Concentrates.
Small quantities of feed samples are collected from several locations, all sides, in the middle and are mixed well after chaffing. Such mixture is subjected to quarter sampling, by spreading on levelled ground in circular form. This circle is now divided into 4 equal quarters. Any 2 diagonally opposite quarters are selected and other 2 are rejected. Selected portions are mixed properly and again spread on levelled ground in circular form. This mixture is again divided into 4 equal quarters and any 2 diagonally opposite quarters are selected, while remaining 2 are rejected. This procedure is repeated till required quantity of sample is obtained. This representative sample is brought to laboratory as early for drying in a hot air oven. Dried samples are ground to pass through 2 mm sieve in a Willey mill (large samples may be ground through hammer mill), as described in chapter 10. Sample is collected in an airtight plastic bag and stored in desiccator.

Life is fundamentally a complex interplay of chemical interactions and reactions. Chemistry examines the composition and properties of matter, the interactions between chemical components, and the mechanisms by which complex structures are formed. Biological chemistry, or Biochemistry, delves into these principles within the context of living systems, highlighting the intricate relationships between molecular structure and function. Understanding fundamental chemistry and its application to anatomy and physiology is crucial because the human body is composed entirely of chemical substances. Basic chemistry encompasses the study of matter, its various states, and energy in all its forms. In order to understand the basic chemistry of living let us start from the scratch of learning and remembering everything that we have learnt so far. Matter and Its Properties Anything with mass and occupying space is referred to as matter. Solid, liquid, and gas are its three main states. Mass, distinct from weight, represents the quantity of matter in an object and remains constant regardless of location. In contrast, weight depends on gravitational force. 

This chapter introduces the chemical composition and nutritional significance of eggs. It examines egg proteins, yolk lipids, triglycerides, phospholipids, cholesterol, vitamins, minerals, trace elements, carbohydrates and pigments. The chapter explains the functional and high-biological-value nature of egg protein and examines bioactive components, digestibility and protein-quality indices. It also considers the relationship between eggs and human health, including the cholesterol debate, amino-acid profile and comparative protein quality. The chapter establishes the nutritional foundation for understanding eggs as an important food resource.

4.1 Introduction

The egg is made up of three primary components: the egg white, the egg yolk, and the egg shell. The calcite crystals of the shell are enmeshed in a protein and carbohydrate complex matrix. About 58% of the weight of an egg is made up of the viscous, colourless liquid termed egg white inside the shell. About 75% of the weight of an egg’s edible component is made up of water, although proteins and lipids make up the majority of its nutritional worth (Table 4.1). There are also trace quantities of minerals, often known as ash, and carbohydrates, which take the form of simple sugars including glucose, sucrose, fructose, lactose, maltose, and galactose.

Chemical immobilization of elephants needs an understanding of itís special features as a priority. Lungs are adhered to the chest wall by fibrous tissue and there is no pleural space. This fact must be taken into consideration when the elephant is cast or put into positions that may inhibit respiration.The trunk of the elephant is a vital organ. An injured or paralyzed trunk is a serious problem; if it cannot be corrected, the elephant will starve.

Chemical restraint is a form of animal restraint technique in which a drug or a chemical is used to restrict the movement (walking, running, aggression) of an animal or sometimes just to sedate or to calm down the animal. Chemical restraint is a safe and effective capture method when applied correctly and with due precaution.

Introduction

Packaging of food material is an essential component of food industry since packaging provides protection, physical tampering resistance and unique appearance to the food products which attracts the customers (Robertson, 2006). The handling of food products would be inefficient and costly if they are not packed properly (Robertson, 2006). The style and design of packaging influence their sale as well as demand. The main aim of packaging is to corroborate the safety and quality of food by preventing microbial contamination (Lee K.M, 2010). Plastics, papers, glass and metals are the common materials used for packaging of food products (Arvanitoyannis and Bosnea, 2004). Among these plastics are most commonly used packaging material because of their low cost, light weight, design freedom and high durability (British Plastic Federation). Various additives are added to the plastics to achieve desired physical and chemical properties. These additives are stabilizers, plasticisers, lubricants and f lame retardants. These additives have potential to transfer from the packaging materials to the food stuffs(K Bunia et al., 2013).

The efficient food packaging solutions are the need of the hour for the Earth. The food losses including the production, post harvest, processing stages of the supply chain and food wastage includes loss of edible food occurring at the f inal stages of food chain that is during retail supply or ultimate consumption. The food born illnesses cannot be ignored which are a consequence of the contamination of food by bacteria, viruses, parasites, new chemical entities or toxicants. We need to optimise the food supply chain, increase food shelf life and consciousness amidst food producers to food consumers. The active and intelligent packaging may help the globe through sufficient food for the increasing population. Scavengers/ absorbers are important solutions which may absorb substances from the packaging inner atmosphere (ethylene absorbers, oxygen scavengers; emitters and antioxidants).

Introduction

The mammary glands of female mammals secrete milk, a necessary food that is liquid and meets the newborn’s nutritional needs. All mammals consider milk to be a complete food for their newborns. It is considered a necessary food for humans and can be consumed as a product or as liquid milk. It contains almost all essential nutrients except iron, which is very low in milk. In essence, milk is an emulsion of protein and fat in water, supplemented with carbohydrates, vitamins, and minerals.

Definition of Milk

• Milk is a complex biological fluid that differs from species to species in both composition and physical properties. It is the full lacteal secretion of a mammary gland that is obtained by milking mammals for a minimum of 72 h after calving or until the milk is free from colostrum.

• FSSAI defines milk as the normal mammary secretion derived from the complete milking of healthy milch animals. It should be free from colostrum.

From a chemical perspective, milk is a dynamically balanced mixture that comes in different forms such as emulsion, colloidal suspension, and true solution. It also contains varying amounts of proteins, fats, carbohydrates, salts, and water.

Gross Composition of Milk

Milk composition varies among different mammals. The average chemical composition of milk in various species is given in Table 1. Buffalo milk and sheep milk contain higher amounts of fat, while human milk comparatively has the highest lactose content. Human milk has lower protein content as compared to milk of other species.

Salinity is a measure of the total concentration of dissolved solids, which is usually expressed in parts per thousand (ppt). Chloride, sulphate, bicarbonate, and bromide are anions (- charged). Cations that are positively charged are sodium, magnesium, calcium, potassium, and strontium. The main solids are sodium and chloride.

This chapter introduces the chemical nature of wool as a keratin-based protein fibre rich in sulphur-containing amino acids. It examines keratin structure, peptide and disulphide bonds, amino-acid composition, amphoteric behaviour and reactions with acids, alkalis, oxidising and reducing agents. The chapter explains the chemical basis of dyeing, swelling, felting, shrinkage and finishing treatments. Understanding these properties is essential for controlling wool processing while preserving fibre strength, appearance and functional performance.