eChapters

Q1.    What is the etiology of Staphylococcus aureus mastitis?
    Ans.    Coagulase positive Staphylococcus aureus.

Q.2.    It causes contagious or environmental mastitis?
    Ans.    Contagious mastitis

Q3.    What are the important animal risk factors of S. aureus mastitis?
    Ans.
    (i)    Presence of coalgulase –ve Staphylococci and Corynebacterium bovis in the mammary gland act as protective factor for S. aureus mastitis.
    (ii)    Abrasion of teat orifice epithelium is a major risk factor of S. aureus mastitis.
    (iii)    Cows with increased parity are more prone to clinical and subclinical S. aureus mastitis.
    (iv)    Presence of different periparturient diseases like parturient peresis, retained placenta, dystocia, ketosis and ulcers in different organs are also risk    factors for S. aureus mastitis.
    (v)    Animals having very low SCC in milk are more prone to S. aureus mastitis.

Sir Alexander Ogston, a physician from Scotland, first elucidated the role of Micrococcus in formation of pus. He proposed the name ‘Staphylococcus’ (In Greek ‘staphyle’ means bunch of grapes and ‘kokkos’ means berry). Anton J. Rosenbach isolated two different species of Staphylococcus and named them according to the pigmented appearance of colonies i.e. Staphylococcus aureus (in Latin ‘aurum’ means gold) and Staphylococcus albus (in Latin ‘albus’ means white). The studies of Bumm (1885) established the relationship of Staphylococcus with human infections. Nocard (1887) and Guillebeau (1890) isolated Staphylococcus from mastitis in sheep and cattle, respectively and established its pathogenic role in animals.

Fill in the blanks

    Q1.    …………… is a contagious pathogen in bovine mastitis
    Ans.    Streptococcus agalactiae

    Q.2.    Streptococcus agalactiae causes ……… (acute/chronic) mastitis if control procedure of mastitis is not used.
    Ans.    Chronic

Answer the following
    Q.1.    Mention the strategy to reduce the prevalence of Streptococcus agalactiae mastitis at herd level.
    Ans.    Good hygienic measures, proper disinfection and efficient treatment of clinical cases.

    Q.2.    What is the relation between SCC and Streptococcus agalactiae infection?
    Ans.    The possibility of Streptococcus agalactiae infection is high with high bulk milk somatic cell count.

    Q.3.    What are the common sources of Streptococcus agalactiae infection in a dairy herd?

Rivolta (1873) first described the chain forming cocci in pus collected from the equine infection. Rosenbach (1884) established the genus Streptococcus’ Nocard and Mollereau (1887) first observed the relationship between the bovine mastitis and Streptococcus agalactiae. Lancefield (1933) classified beta-haemolytic streptococci on the basis of group specific-carbohydrate antigens.

Properties of genus

1. Morphology: Streptococci are gram positive cocci, capsulated, non-sporing and non-motile. Each coccus cell is 1µm in diameter. They are arranged in a pair or chain of cells as the cell division occurs in one plane(Figure 1 & 22). The chain formation and the chain length are variable. Streptococcus equi ssp. equi is a constant chain producer. Young cultures are gram positive whereas bacteria present in the pus and older cultures in the laboratory are often stained as gram negative.

Streptomyces are soil-borne bacteria known for production of several antibiotics, antifungals, antivirals, antitumoral, anti-hypertensives and immunosuppressives. Krainsky (1914) first described S. griseus and named the species Actinomyces griseus. Waksman and Henrici (1948) changed the name to Streptomyces griseus.

Dermatophilus congolensis was first described from clinical case of exudative dermatitis in cattle in Congo by Van Saceghem (1915). It is primarily known as an animal pathogen causing acute, sub acute or chronic skin disease. The first cases of human infection with D. congolensis were reported in 1961 in four people who developed skin infection after handling an infected deer in USA.

Since the periods of Hippocrates and Lord Buddha cholera like symptoms was described in human. The first outbreak of cholera was described in India in 1817. Filippo Pacini (1854), an Italian anatomist, observed a gram-negative bacterium associated with human cholera. Robert Koch (1884) first isolated and confirmed the association of ‘comma-shaped bacteria’, later designated as Vibrio cholerae with the disease in Calcutta (India). In 1959, Sambhu Nath De, Professor of Pathology at Nilratan Sircar Medical College in Calcutta discovered the exotoxin produced by V. cholerae and a simple method (rabbit ligated ileal loop assay) to study the in vitro effect of exotoxins. The Ganges delta of India and Bangladesh is considered as the worst affected place with cholera although the infection is widespread throughout the world. The spread of cholera in different continents occurred through six ‘waves’ (‘pandemics’) since 1817. The current seventh pandemic (7P) was originated in Indonesia (Sulawesi) in 1961 and spread throughout Asia, Africa and Latin America. In 1963, the seventh pandemic reached South Asia and circulated for more than 50 years with intermittent transmission to other continents. The recent major 7^th pandemic outbreak was detected in Haiti causing more than 9400 deaths in 2010 which was originated through patients from endemic countries.

In 1886, Escherich observed Campylobacter like organisms in stool samples of children with diarrhoea. In 1913, McFaydean and Stockman first confirmed the bacteria as Campylobacter in foetal tissues of aborted sheep. Confirmatory tests were also carried out by Smith in 1918 when similar bacteria were isolated from aborted bovine foetuses. The bacteria were then known as Vibrio foetus. In 1963, due to certain differentiating characteristics, the organisms were separated from Vibrionaceae family and the new genus Campylobacter (means ‘curved rod’) under Campylobacteraceae family was proposed.

The following objectives are accomplished by semen dilution/ extension

• Preservation of semen potential over a long period of time.
• Increase in the number of services per ejaculate. Ideally approximately 40 does can be inseminated from diluted/ extended semen of a single ejaculate.
• Extenders provide the spermatozoa with a medium in which they are able to remain viable and fertile for a prolonged time period. An ideal extender should be able to provide a conducive environment to facilitate sperm survival and modify the sperm structure to prepare them for freezing through the following properties:
• It should be isotonic with blood and should maintain this during preservation.
• It should have capacity of maintenance of pH (6.6-6.8) with high buffering capacity in that range.

Goats remain an important and instant source of income as well as livelihood for the majority (70-90 %) of small and marginal farmers in India. Goats are the animals with multiple utility like milk, meat, hide, fibre and manure. They are more tolerant to harsh environment (better resistance to heat stress and drought, better utilization and digestibility of pastures) and give more production per unit investment. Goats are spontaneously ovulating, poly-estrous animals and show seasonal pattern in reproductive activity related to the annual variations of photoperiod. Onset and length of their breeding period throughout the year is dependent on different environmental and physiological factors viz. latitude and climate, feed and fodder availability, breed and breeding system. This in turn affects round the year availability of meat and milk to full fill the market demands. When kids are born out of season, adequate supply of pastures along with good management practices must be ensured in order to minimize kid’s mortality and promote their optimum growth.

Abstract
In-silico analysis of nucleic acids and proteins from fishes has enriched our understanding to several fundamental problems of classical fish biology and genetics. Mining of data which is analogous to information discovery is the process of automating knowledge discovery. Analysis of data in all possible manner and recognition of a pattern which has a scientific basis by an efficient algorithm is the fundamental principle of Bioinformatics. Ever increasing number of Bioinformatics tools as computational packages are now a days used in tandem to analyze sequence of a loci and to model a novel gene product. High efficiency algorithms with excellent homology searching functions have lead to this advancement. Cat fishes, a group of diverse bony fishes have been studied with respect to genetic analysis in several species and several peptides. Tracking of the evolutionary foot prints using genomic libraries and between expressed sequences has been generating much interest.

Mammals are homeothermic, meaning that they have the ability to control their body temperature within a narrow range in an environment whose temperature may change over a wide range.

India is experiencing a phenomenal spurt in the growth of livestock and poultry sectors through various timely and quality measures scientifically over a few decades. Milk production rose by a large leap from about 17.0 million tones in 1950 to about nearly 100 million tones in 2005 standing first in the world for maximum quantum of milk production. From about a ‘naught’ during 1950, the commercial layers have increased to 220 millions in 2005 producing 4500 crores of eggs every yearranking 5th in the world. The meat production is absolutely fascinating with 20 million birds in 1960 to about 2100 million in 2005 (Table 10.1).

Increase in ambient temperature, drought, rainfall, desert, feed and fodder scarcity, water scarcity and other climatic stresses are very common to tropical climatic conditions. The animals living in tropical climate have to undergo some modification for their survival under such extreme conditions. Interestingly, higher percentage of livestock is found in tropical and sub-tropical regions of the world. These areas are rich in animal resources. There are several descriptive and non-descriptive breeds of bovine distributed in different agro-climatic conditions of India. They possess several traits which make them adaptive to such climatic conditions. Identifying those traits and propagation of such traits is beneficial in near future in respect of climate change scenario. Climate change is the major concern for the improvement and sustainability of livestock under tropical climatic conditions. The rising of temperature and humidity is one of the major threats to animal’s productive performance that leads to great economic losses. Upadhyay et al. (2009) estimated 2% loss of milk production due to thermal stress in India. It has also been expected that 3.2 million tons reduction by 2020 and more than 15 million tons by 2050. The decline in milk production reported to be higher in crossbreds (0.63%) followed by buffaloes (0.5%) and indigenous cattle (0.4%) (Upadhyay et al., 2009). 

The climatic changes and global warming may impact the economic viability of livestock production systems in a significant manner. The possible consequences of increased ambient temperature on the livestock include reduced feed intake, reduced weight gains, lower milk production and lower conception rates during summer periods. Though animals can, to some extent, adapt to higher temperatures with prolonged exposure, the production losses are likely to be exhibited in response to higher temperature events. However, potential direct and indirect impacts of climate change on livestock production have not yet been thoroughly explored. Changes in availability and quality of fodder have also been identified as one of the major impacts of climate changes leading to lowered productivity of livestock species. Climate change could also affect the distribution of vector-borne livestock diseases. These changes occur as a result of shifts in the geographical ranges of ticks, mosquitoes, flies and other vectors.

Introduction

Spatial planning tools brings together multiple users of the earth to make informed and coordinated decisions about how to use of resources  and helps to organize, analyze and present information from a number of different sources.  Thus, viewing from single interest or multiple use viewpoints is enhanced and can make a very important contribution to environment. Use of Remote Sensing (RS) and Geographical Information Systems (GIS) tools has the capacity to bring together experts from a variety of disciplines to address complex spatial problems.

Introduction
Science and its development have had a great impact on the biological research. The main problem arises to understand and interpret the data which are the results of the higher dimensional research in the above mentioned field, and it is extremely difficult some times to give answers for complex and tedious biological questions generated through the large datasets. Parallel developments of statistical methods provide relaxation over these issues. The statistical method by bioinformatics presents many new and logical problems for researchers, scientists, and scholars related to these communities. Thus the knowledge of statistical concepts and techniques are prime important in this chain. The effort is made to cover stochastic theory, finite Markov chains in learning models to diffusion processes in population genetics, advanced statistical methods with Hidden Markov model and provide some literature on Bayesian computation like MCMC and their resources. Fortunately the applied aspect of probability theory and theory of stochastic processes seem to have been extensively used in the research and development level of biological field but the lacking of required material is a very problematic aspect in the system with proper and systematic ways.

All living organisms need to reproduce in order to maintain their species and improve the economics of animal husbandry. Since the beginning of time, different methods of successful reproduction have been created, including artificial insemination and in vitro fertilization. To shield sperm from damaging elements like freezing, osmotic shock, oxidative stress, and cell damage by ice crystals, semen extenders were developed. Semen extenders maintain sperm’s characteristics, such as its morphology, motility, and viability as well as its membrane, acrosomal, and DNA integrity. To improve semen quality for fertilization, semen extenders must offer a suitable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity. As a result, this compilation of research offers exact information on various semen extenders, preservation mechanisms, and crucial semen extender additions in goats. By incorporating various types of additives in semen extenders for cryopreservation, significant improvement is reported in post thaw sperm quality in terms of increased % motility, viability, PMAI, MMP and reduced DNA fragmentation, capacitation like changes reflected by less CTC pattern B and AR, Tyrosine Phosphorylated pattern A, E and AE.

The greenhouse effect is thought to be due to the absorption of solar infrared (IR) radiation by gases and the earth’s surfaces, which, as a result, is heated and then reemit IR radiation at low frequency with a high absorptive power. In fact greenhouse gases in the atmosphere are essential for maintaining life on earth, as without them the planet would be permanently frozen because all of the incoming heat from the sun would be radiated back into space by the earth’s surface (Moss, 1993). The threshold concentration of these gases at which their greenhouse effect would be minimized is not known, but it is accepted that their concentrations in the atmosphere should not be allowed to continue to rise. In recent years, there has been an increase in public concern over environmental damage originating from animal feeding operations.

Introduction

The proteome is often described as the set of all expressed proteins in a cell, tissue or organism. It is the systematic analysis of cellular proteins, that is, their identity, quantity and function. One advantage of proteomics over transcriptome is that mRNA represents an early stage of gene expression whereas the study at the proteome level describes the state of protein which is the final functional molecule of the cell. Thus, it gives a clear picture of the cell function that may not be concluded from mRNA study. In fact we can say that proteins are more than just information carriers so it gives more information with respect to only nucleic acid based studies.  Proteomics covers two-dimensional gel electrophoresis (2-DE), image analysis, mass spectrometry, amino acid sequencing, and bio-informatics to resolve comprehensively, to quantify, and to characterize proteins. Biomarker discovery as an important aspect of proteomics technology is highly appreciable when it comes to diseases. Today,  a better understanding of the disease mechanisms leading to discovery of new disease specific biomarkers are very much possible due to the sequencing of the human genome. It has accelerated the proteomic technologies for accepting the challenges so as to meet the diseased related investigations.

In recent past, extreme weather events are causing great concern in different sectors contributing to Indian economy. India Meteorological Department is doing yeomen’s service by providing advance information including monitoring of the extreme events longs with proper advisories to the farming community by using state of art instruments & technology through efficient delivering mechanism of the information and ultimately help the farmers from incurring great loss. In many cases, the most severe impacts are felt when several extreme events occur together. The major extreme weather events related to agriculture are Tropical cyclones, floods, heavy rain and landslides, cold waves, fog, snow storms, Heat waves, Hailstorms, thunderstorms and dust storms, drought.

The analysis of chromosome behaviour at meiosis can provide valuable information about the genomic composition of both species and hybrids. The basis for this analysis is the idea that chromosomes that pair and undergo chiasma formation are homologous. Where two genomes are present, as in diploids, the expectation is that only bivalents will be observed and where there are multiple genomes, as in polyploids, two general patterns of pairing are possible. In autopolyploids, configurations of multiple chromosomes are to be expected since all the component genomes are homologous and have the possibility of pairing with each other, whereas in allopolyploids, with different genomes, bivalent formation is the expectation. Thus, meiotic analysis can provide an oversight for genome analysis to investigate genetic relationships between the species. Further, chromosomal rearrangements are correlated with pollen fertility in species and F₁ hybrids.  Observations on pollen formation, size and viability can provide useful information, as low pollen viability is often indicative of meiotic irregularities and large sized pollen can indicate the formation of unreduced gametes. In addition, the occurrence of nucleolar dominance has been recorded in many interspecific hybrids.

Computation has become an essential tool in life science research. This is exemplified in genomics, where first microarrays and now massively parallel DNA sequencing have enabled a variety of genome-wide functional assays that require increasingly complex analysis tools. The problem of accessibility of computational tools has long been recognized. Without programming or informatics expertise, scientists needing to use computational approaches are impeded by problems ranging from tool installation; to determining which parameter values to use; to efficiently combining multiple tools together in an analysis chain. The severity of these problems is evidenced by the numerous solutions to address them. Kowledge on software  and web-based interfaces for tools all improve the accessibility of computation. These approaches each have advantages, but do not offer a general solution that enables a computational tool to be easily included in an analysis chain and run by scientists without programming experience.However, making tools accessible does not necessarily address the crucial problem of reproducibility.