eChapters

The tentative diagnosis of viral diseases of livestock can be made by clinical symptoms alone. The clinical diagnosis is substantiated by the laboratory to obtain a precise, correct and prompt diagnosis. The success however depends on the collection of suitable materials in proper transport medium at a particular stage of disease condition and its dispatch to the laboratory in a manner that little or no alterations occurs in the specimen. This would facilitate the isolation and identification of the causative viral agents easily.

On the face of an outbreak of a disease, collection of right clinical specimens in proper preservative, their transportation to the laboratory under ideal conditions and quick processing of specimens in the laboratory to identify the causative agent are crucial to successful control of the disease. This attains more importance when a highly contagious disease appears.

Collection of specimens from a virus-infected host is the first step for demonstration or isolation of a virus. Specimens for virus isolation should be collected at the time when the maximum amount of virus can be expected to be present. i.e. collect the specimens as early as possible after the onset of clinical symptoms. In many viral diseases maximum viral production occurs before the patient becomes ill so that it is ideal to collect the specimen at an even early time.
The nature of the specimen taken in any particular instance is determined by the patient’s clinical syndrome and previous history. They are best considered under three main headings.

1. Specimen for virus isolation/antigen detection/ nucleic acid detection
2. Specimen for serological investigation
3. Specimen for direct examination

To identify parasitic infections, it is essential to examine excretory- secretory products (ESPs), viz., faeces, urine, sputum and nasal discharges as examination of such material is essential for clinical diagnosis. It is extremely rare to get an animal completely free from parasites and at the same time all animals may not show signs of disease. Most of the important helminths and coccidia inhabit intestinal tract. Therefore, clinical diagnosis of gut dwelling helminths and coccidian depends mostly on identification of ova/oocyst in faecal samples

Trace minerals have long been identified as essential components in the diets of domestic livestock species. Trace minerals exist in cells and tissues of the animal body in a variety of chemical combinations, and in characteristic concentrations, depending on the trace mineral consumed and the tissue in which it is metabolized. Concentrations of trace minerals must be maintained within narrow limits if the functional and structural integrity of the tissue is to be maintained and the growth, health, and productivity of the animal are to remain unimpaired. Minerals are as vital to cattle as grass, water or air.

Introduction

The quality of interaction between clinicians and microbiologists has an enormous influence on the effectiveness of the laboratory service. An accurate diagnosis is based on the interpretation of both clinical and laboratory data. Investigation of disease is solely dependent on the quality and appropriateness of the specimens collected. The diagnosis depends on the skill and care with which the clinicians select, collect and transport the specimen to the laboratory. Clinical history, including the tentative diagnosis, should always accompany the specimen. If available, a detailed post-mortem report must also be sent. These set of information will help the microbiologist to select the most appropriate procedure and to furnish a meaningful interpretation of the results.

Specimens need to be collected for establishing a disease diagnosis or monitoring of vaccine response or surveillance. The knowledge of the pathogenesis of the infectious disease is the most important factor for determining the most suitable specimen. The samples need to be appropriate, and adequate in number and amount to provide a statistically valid result. Samples must be taken with care, to avoid undue stress or damage to the bird or danger to the operator. Careful consideration must be given to the collection, containment, and storage of the specimens, including biosafety measures to prevent spillage to the environment or exposure of other birds and humans.

Hard tongue due to fibrous tissue proliferation (termed as wooden tongue) is a characteristic feature.

Swab and smear from abscess and tissue samples including tongue and lymphnodes.

Steps in the Estimation of Trace Elements
Estimation of trace elements in feeds and fodders and other biological materials involves the following steps :

    1.    Production of an acidic solution of the inorganic elements in the biological samples after removal of the organic matter by dry ashing or wet oxidation.

    2.    Removal or masking of interfering substances.

    3.    Determination of the selected elements, usually by using colorimetry.

Results of trace elements determinations are expressed as parts per million (ppm), mg/kg or µg/g dry matter.

Introduction

Colorimetry is a scientific technique widely used across various fields to quantify the concentration of substances based on their color intensity. Colorimetry is a branch of analytical chemistry that focuses on measuring the absorbance or transmission of light by colored solutions. It utilizes the principles of optics and spectroscopy to determine the concentration of a substance in a solution based on its color intensity. This technique relies on the Beer-Lambert law, which states that the absorbance of light by a solution is directly proportional to its concentration and path length.

Principles of Colorimetry

The fundamental principle of colorimetry lies in the interaction of light with matter. When light passes through or is reflected off a substance, certain wavelengths are absorbed, while others are transmitted or reflected. The color we perceive is due to the wavelengths of light that are reflected back to our eyes. In colorimetry, a photometric measurement is made using a colorimeter or spectrophotometer, which quantifies the intensity of light absorbed or transmitted by a sample.

Introduction Colostrum feeding • Colostrum is rich source of gamma globulins (antibodies), vitamins and minerals • Colostrum Temp.: 99ºF-102ºF • Colostrum feeding: first 3-5 days. • Whole milk: after 5 days • Feed the total amount of milk at 3 or 4 equal intervals up to the age of 7 days and then twice daily • Quantity of colostrum: 10 % of body weight. • Overfeeding: calf scour/diarrhea. • Around 0.5% of a cow’s yearly milk production is made up of colostrum. • After birth, the amount of immunoglobulins that can be absorbed from colostrum drops by 1/3 as soon as 6 hours, by 2/3 after 12 hours, and an intestinal barrier forms after 24 hours. • Colostrum must be given in sufficient amounts to the newborn calf because during the first 24 hours after parturition, the permeability of the stomach and the concentration of immunoglobulins drop sharply. • Cow colostrum must meet minimum requirements for both immunological and bacteriological characteristics:

Poultry industry in India has registered a phenomenal growth during the last four decades making it one among the world leaders in poultry production. The development of organized poultry has in fact masked the contribution of backyard poultry or house-hold poultry of rural sector. Backyard poultry production in India is characterized by small flock size consisting of 5-10 predominantly non-descript birds maintained in extensive system under zero input conditions but fetch the owners much needed animal protein and supplementary income. Importance of backyard poultry is well recognized by Government of India and special programmes are formulated for its promotion.

Non-descript Chicken

Maintaining small flocks of local non-descript fowls under free range condition by landless poor, small and marginal farmers are a common sight in rural areas. These birds are very popular due to their adaptability to local agro climatic conditions and management practices with prominent brooding behaviour and mothering ability. But these birds are small in size, poor layers (40-50 eggs/ annum), with small clutch size and intense brooding behavior, thus making them not suitable for commercial exploitation.

Celite Column Chromatography
(Method of Wiseman and Irvin, 1957)
Principle

Celite columns with an internal indicator have been developed for the quantitative separation of silage and rumen volatile fatty acids ranging from butyric to succinic. The aqueous phase employs minimal amounts of sulphuric acid to prevent retention of organic acids, sugar is added to the phase to increase elution resistance. Aqueous samples, 2 ml or less are added directly to a dry column cap. Eluting solvents are mixtures of acetone and petroleum ether. Single zone collections make possible a reduced number of titrations with increased accuracy. The column permits fairly wide separations of lactic acids, often difficult on silicic acid columns.

The method employs celite with alphamine red R as an internal indicator. A distinctive feature is the use of concentrated sugar solution as the stationary phase to resist leaching action of solvents.

Wet litter can be caused by extrinsic or intrinsic factors related to excessive excretion of water. When the moisture content of litter exceeds 35%, it can lead to various issues, including pododermatitis of the foot pads, resulting in poor growth rate in broilers and low fertility in breeder flocks. Wet litter can also contribute to inflammation of the feather follicles. This can lead to gangrenous dermatitis, which is responsible for downgrading at processing or rejection by consumers. Additionally, wet litter is often associated with outbreaks of coccidiosis, as the oocysts of Eimeria spp. require moisture levels over 25% to mature. Necrotic enteritis occurs frequently in houses with areas of wet litter, while damp litter may contribute to the proliferation of toxic fungi.

Introduction

The impacts on the various facets of life – agriculture and food processing – need no longer be addressed in a way that is well established. Nearly every aspect of the planet has shown its effect at different rates, both positively and negatively. As a subtropical region, India is also witnessing different variations in weather and an increase in temperature that affect farming. Only in recent years has the potential to reduce transpiration from plants by the use of chemicals been further explored. These antitranspirants can either control perspiration by filming the leaf surface or by manipulating the aperture of the stomach. Antitranspirants are the materials or chemicals that reduce the water loss from plant leaves by reducing stomata size and number. During transpiration, almost 99 per cent of the water consumed by the plant is lost.

Introduction

Dairy is vital to the economics of many developing countries. Animals are a source of food, more specifically protein for human diets, income, employment and possibly foreign exchange. For low income producers, dairy can serve as a store of wealth; provide draught power and organic fertilizer for crop production and a means of transport. Consumption of dairy and dairy products in developing countries, though starting from a low base, is growing rapidly. Dairying has become an important secondary source of income for millions of rural families and has assumed a most important role in providing employment and income generating opportunity. Indian Dairying is unique in more than one ways. It ranks first with its 199.1 million cattle & 105.3 million buffaloes accounting for about 51 percent of Asia’s and about 19 per cent of world’s bovine population. It also ranks first in milk production with a production of 121.8 million tones in 2010-11 and the demand is expected to be 180 million tones by 2020. To achieve this demand annual growth rate in milk production has to be increased from the present 2.5 % to 5%. Thus, there is a tremendous scope/potential for increasing the milk production through profitable dairy farming. Besides milk, the manure from animals provides a good source of organic matter for improving soil fertility and crop yields.

The profitability of poultry farming primarily depends on reduction in feed cost, which can be achieved by utilizing optimum concentrations of energy and crude protein (CP) from economically viable and nutritionally balanced feed ingredients. Soya bean meal (SBM) is conventionally used as a source of protein in poultry diet. However, the shortage and escalating cost of this prime protein source makes poultry farming uneconomical in many developing countries. Continuous efforts are, therefore, is on in search of viable alternate protein feed ingredients for SBM. 

4.1 Layer Industry
4.1.1 Global scenario
Poultry is raised by approximately 80 percent of rural households in developing countries. Food and Agriculture Organization’s statistics department show that total egg production has grown from 61.7 million tonnes in 2008 to 76.7 million tonnes in 2018. China continues to lead the way, producing 466 billion eggs in 2018, which represents 34 percent of the global market. China has been the world’s largest producer of eggs for the last 30 years. China, European Union (EU), United states of America (USA) and India produce almost 60 percent of the world’s eggs, while the next 6 largest producers take a further 16 percent of the market. This means that the top 10 egg producers account for more than three fourth of the world’s egg production. Asia is the largest eggproducing region, with more than 60 percent of global output. 

Chocolate is a key ingredient in many foods such as milk shakes, candy bars, cookies and cereals. It is ranked as one of the most favourite flavours in North America and Europe. Despite its popularity, most people do not know the unique or igins of this popular treat. Chocolate is a product that requires complex procedures to produce. The process involves harvesting coca, refining coca to cocoa beans, and shipping the cocoa beans to the manufacturing factory for cleaning, conching and grinding. These cocoa beans will then be imported or exported to other countries and be transformed into different type of chocolate products.

In history, 1828 marked the “modern era” of chocolate making when Dutch chocolate maker Conrad J. van Houten patented an inexpensive method for pressing the fat from roasted cacao beans along with other processes to create a fine powder known as “cocoa”. The powder was then treated with alkaline salt that helped the powder mix with water easily. The creation of powdered chocolate made it easier to mix with water, sugar and a possible combination of other ingredients to make chocolate in solid form. Many other chocolate makers began to build on Van Houten’s success to make a variety of chocolate products. In 1894, English chocolate maker Joseph Storrs Fry produced what was arguably the world’s first eating chocolate”

Commercial Advertising

In today’s highly competitive market, businesses rely on advertising as a vital tool to promote their products and services. Commercial advertising plays a crucial role in shaping consumer perceptions, driving sales, and building brand identity. It is a strategic communication process designed to inform, persuade, and remind potential customers about a company’s offerings. This chapter explores the definition, objectives, types, strategies, and impact of commercial advertising in modern business environments.

Definition of Commercial Advertising

Commercial advertising refers to paid marketing communications that aim to promote a product, service, or brand to consumers. It is executed through various media channels, including television, radio, print, online platforms, and social media. The primary goal of commercial advertising is to increase awareness and influence consumer purchasing decisions.

Breeds of rabbits: There is a diverse range of breeds when it comes to domestic rabbits, with each one possessing unique qualities. Currently, there are 38 recognized breeds and 87 varieties of rabbits that are well established all around the world. These breeds and varieties differ in various aspects such as color, size, type of hair coat, and other characteristics. Depending on their purpose, rabbits can be categorized into three groups: those bred for meat production, those bred for fur production, and those kept as companion animals.

Milk, on reception at the dairy factory is subjected to various unit operations on its way to product manufacture. Processing is applied as a means to convert liquid milk into products or to render the milk (and products) safe from harmful organisms. The basic processing operations to which milk is subjected at processing units have been described in this chapter. The common processing operations employed are depicted in Fig. 4.1. Several of these operations are routine compulsory processes common to all dairies, while certain others are undertaken for specialized purposes in some dairies.

4.1. Filtration and Clarification

At the time of milking various foreign materials such as dirt, feed, manure, dandruff, hair, flies, dust particles, worn out cells, cell debris and many other undesirable substances may find their way into milk. The process of removal of this extraneous matter (solid particles of higher density than that of milk) is called filtration or clarification. This can be achieved either by simple filtration through specially woven filter cloth or continuous process of centrifugal clarification that helps