eChapters

Dr. Chauhan is an exceptional individual who had achieved a significant milestone in his academic and professional journey. Completing a Bachelor of Science (Honours) with an impressive 80.3%, Dr. Chauhan’s accomplishment is a reflection of his hard work, dedication, and unyielding passion for the field of veterinary science. This remarkable achievement not only underscores his
academic brilliance but also cements his position as an inspiring figure in the veterinary community.
From an early age, Dr. Chauhan demonstrated an innate love for animals and a deep sense of responsibility toward their well-being. These qualities formed the foundation of his journey in veterinary science. His BVSc&AH (Honours) degree, completed with such distinction, is not just a testament to his intellectual capabilities but also to his tireless commitment to improving animal health and welfare. Pursuing a degree in Veterinary science is no small feat. It demands a unique combination of scientific expertise, practical skills, and emotional resilience. For Dr. Chauhan, the rigorous academic journey involved mastering a wide array of subjects, including animal Anatomy, Physiology, Pathology, Pharmacology, and Microbiology. Each subject posed its own set of challenges, yet he tackled them with unwavering determination and an insatiable thirst for knowledge. His exemplary performance, as evidenced by his impressive score of 80.3%, highlights his ability to excel even under pressure. What sets Dr. Chauhan apart is not just his academic excellence but also his holistic approach to learning. Beyond textbooks and lectures, he actively sought opportunities to gain hands-on experience, volunteering at animal shelters and participating in community outreach programs. These experiences not only enriched his understanding of animal care but also shaped his perspective as a compassionate and empathetic veterinary professional.

Estimation of Cellulose in Feeds and Fodders
Principle

The feed well ground is digested with acetic acid which dissolves all other constituents like protein, disaccharides, fatty acids, etc., and cellulose and mineral contents are unaffected. The residue is made acid and alcohol free by washings. Alcohol removes water, benzene removes pigments and fat and at the end the sample is dried and ashed. The difference between the dried weight before ashing and weight after ashing gives cellulose content of the feed sample.

Poultry sector is expected to play the most crucial role in transforming the nutritional demography of country with ever growing share of protein based cheap human diets. Some of the specific challenges the poultry sector is faced with are as follows:
a) Regional imbalances in poultry production
The commercial layer farming is yet to make a dent in some of the region of country. However, broiler farming is gradually catching up in majority region.
b) Underexploited poultry diversity
Among poultry species, chicken production has already acquired large scale commercial dimensions in some states due to its better efficiency. However, there is a scope for exploitation of other domesticated poultry species.
c) Rising feed cost
The growth in poultry has outpaced the growth in cereal production leading to severe feed shortages and consequent rise in feed cost. Cereal by-products and oilseed residues usually constitute more than 80 percent of poultry diet. Coarse cereals also form the staple diet of millions of marginal farmers and landless labourers. These coarse cereals are the most important and most widely used poultry feed ingredients all over the world. The demand for coarse cereals is continuously increasing at 4 percent per year due to ever increasing population and their use in livestock rations.

Introduction

The camel is an important animal part of the desert eco-system. With its unique characteristics, the camel has becomes adapted to tough way of living in arid and semi arid region. The camel called “Ship of desert”, because he can walk across the desert, even on a hot afternoon and Hot sand also can’t affect it. He can run on hot sand@ 25 km per hour with his thick and padded feet, so hot sun does not burn them. In desert, there are no rivers or lakes, then also it can’t affect to camel because he can drink 46 litres of water at a one time. He can eat a lot of food at one time, so he doesn’t need to eat for several days. Camel eats leaves, but there are no trees in a desert, so he can eat thorns with the help of thick lips and thick tongue. Camels in India are reared under extensive type of system. Camels are also kept in small numbers under intensive system, particularly for the routine work such as camel cart traction, transport for men and materials, agriculture operation. Seasonal movement of camel herds to the areas having comparatively better pasture facilities is a quite common practice. A camel is even toed ungulates that having distinctive fatty deposit on its back (hump). Camels have been domesticated as livestock. They provide food (milk + meat) and textile (fibres and felt from hairs). Camels are working animals especially suited to desert habitat as vital means of transport for passengers and goods. There are three species of camels.

The term “chelate” comes from the Greek word “chel,” meaning “claw,” indicating that the element is tightly held or clawed by the ligand. The term was coined by Morgan and Drew in 1920, defining it as a chemical compound in the form of a heterocyclic ring with a metal ion attached by coordinate bonds to at least two non-metal ions, known as chelation. A chelate is a type of mineral complex, which is a mixture of a mineral and an organic compound like a protein or polysaccharide. Chelation refers to the bond formed between a metal ion (mineral) and a ligand (protein or amino acid chelating agent). Chelates are created by reacting a mineral salt with an enzymatically prepared mixture of amino acids and small peptides under controlled conditions. The ligand binds the metal at multiple points, forming a ring structure with the mineral, resulting in a chelate.
In animal feed, chelated minerals, such as iron, manganese, cobalt, copper, and zinc, are commonly used to enhance bioavailability and support metabolic functions. These trace elements are transitional elements on the Periodic Table, forming stable complexes or chelates due to their ability to form coordinate covalent bonds.
The primary goal of chelates is to improve mineral absorption in animals during periods of high nutritional demand, such as pregnancy, lactation, weaning, and environmental or disease stress. Chelates have been studied for their effects on immunity, reproductive performance, and overall herd health. To maximize mineral absorption, chelated compounds should be stable in the rumen.
Chelates are stable, electrically neutral complexes that protect trace minerals from chemical reactions during digestion, ensuring their availability to the animal. Chelated minerals are used to increase bioavailability and uptake, reaching the plasma intact and separating at the site of action. They can replace a portion of inorganic minerals in animal diets and are suitable for all species. Soluble chelated trace minerals are designed for use in drinking water supplies.

Preparation of Samples

Before withdrawing portions for analytical determinations, bring the sample to a temperature of 26 to 28ºC and mix throughly by pouring gently into a clean dry receptacle and back, until a homogenous mixture is assured. If lumps of cream do not completely disappear, warm the sample to about 40ºC, mix throughly, then cool to 26 to 28ºC. In case a measured volume is required in a determination, bring the temperature of the sample to about 27ºC before pipetting. All samples shall be allowed to stand for three to four minutes after mixing to allow air bubbles to rise ; the sample bottle shall then be inverted three or four times immediately prior to taking the milk for analysis. If a sample is curdled, excessively churned or decomposed, the sample should not be taken up for analysis.

The chemical composition and digestibility of feedstuffs influence their nutritive value for animal production. Therefore, assessment of nutritive value of feedstuffs continues to be one of the top priority areas in animal production research. Although traditionally the feedstuffs were evaluated by performance studies and digestion trials, such techniques are expensive and requires more time and labour. Continued search for alternatives to animal feeding experiments have led to the development of feed evaluation systems that are rapid and economical.

In general, there are two kinds of laboratory analyses — Chemical analyses and Biological tests with rumen micro-organisms or purified enzymes. Biological tests give direct estimates of digestibility, but are lengthier and more expensive. Chemical analyses are cheaper and rapid, but do not give direct estimates of nutritive value. The statistical association between the chemical components and quality need to be established to predict the nutritive value.

Preparation and processing of samples for chemical analysis: The preparation of samples depends upon the purpose of analysis and the nature of the constituents that are to be determined. The preparation of samples for analysis is as important as the analytical procedure and hence, it should be done with utmost care. (A) Smpling of wet material: (1) Plant material; (2) Silage; (3) Cattle faeces; (4) Sheep / goat faeces; (5) Poultry excreta; (B) Sampling of air – dry material: (I) Hay or straw; (II) Concentrates.
Small quantities of feed samples are collected from several locations, all sides, in the middle and are mixed well after chaffing. Such mixture is subjected to quarter sampling, by spreading on levelled ground in circular form. This circle is now divided into 4 equal quarters. Any 2 diagonally opposite quarters are selected and other 2 are rejected. Selected portions are mixed properly and again spread on levelled ground in circular form. This mixture is again divided into 4 equal quarters and any 2 diagonally opposite quarters are selected, while remaining 2 are rejected. This procedure is repeated till required quantity of sample is obtained. This representative sample is brought to laboratory as early for drying in a hot air oven. Dried samples are ground to pass through 2 mm sieve in a Willey mill (large samples may be ground through hammer mill), as described in chapter 10. Sample is collected in an airtight plastic bag and stored in desiccator.

This chapter introduces the chemical composition and nutritional significance of eggs. It examines egg proteins, yolk lipids, triglycerides, phospholipids, cholesterol, vitamins, minerals, trace elements, carbohydrates and pigments. The chapter explains the functional and high-biological-value nature of egg protein and examines bioactive components, digestibility and protein-quality indices. It also considers the relationship between eggs and human health, including the cholesterol debate, amino-acid profile and comparative protein quality. The chapter establishes the nutritional foundation for understanding eggs as an important food resource.

This chapter introduces the chemical nature of wool as a keratin-based protein fibre rich in sulphur-containing amino acids. It examines keratin structure, peptide and disulphide bonds, amino-acid composition, amphoteric behaviour and reactions with acids, alkalis, oxidising and reducing agents. The chapter explains the chemical basis of dyeing, swelling, felting, shrinkage and finishing treatments. Understanding these properties is essential for controlling wool processing while preserving fibre strength, appearance and functional performance.

Silage fermentation is a complex process involving biochemical changes by microbial populations and the ensiling environment. The chemistry and microbiology of silage fermentation are linked, but the microorganisms are the dominant factor in the fermentation process, and they have the main influence on the quality of the end product. Many species of microorganisms are involved in the fermentation process, and their relative importance varies according to the prevailing conditions. As these conditions change during the fermentation process, the microorganism population changes accordingly. Lactic acid bacteria and clostridia are two main categories of microbes responsible for biochemical changes in silage.

Artificial insemination (AI) technology is one of the important managemental tools for the successful poultry industry. It has several uses and a few limitations also. This technique is performed to avoid the spread of venereal diseases by natural mating and to increase the dissemination of genetic material to a large number of birds. This technique include: semen collection, semen evaluation (physical and biochemical characteristics), semen dilution and preservation and deposition of semen in the female genitalia. Semen dilution is one of the most critical components of AI technology. Several suitable semen dilutors are reviewed for low temperature storage up to around 48 hours.

Chilli thrips: Scirtothrips dorsalis, Thrips palmi: Thripidae

The adults of this insect are small brownish black with fringed wings. The nymphs are slender and yellowish straw coloured, later acquiring more brownish hue. They lacerate the tissues and feed on the exuding sap. Leaves become crinkled, curled upward and shed. The attack can be confused with chilli mosaic symptom.  In capsicum and chilli, they also feed beneath calyx of flowers and fruits causing flower/fruit drop and reduction in yield and market value of capsicum. Buds become brittle and drop down. Plants get stunted and bronzed. The fruits if at all formed are crinkled and small.

Chrysomelid beetle : Aulacophora foveicollis : Chrysomelidae

Adults cut tender shoots and feed on leaves resulting in holes and loss of leaf. Grubs feed on roots and underground stems leading to yellowing,wilting and drying of attacked plants.

Leaf-and shoot folders: Hymenia recurvalis : Pyraustidae

Pycnarmon cribrata;Phostria piasusalis

Nutrition plays a pivotal role for the animal research, be it health or production. Research in animal nutrition employs many basic techniques and chromatography is one of them. Chromatographic processes can be defined as separation techniques involving mass transfer between stationary and mobile phases. Various types of chromatography have one application or the other in analysis of food/feed components (Day and Williamson, 2001), quantitation of nutrients as well as the characterization of the effect of nutrients in biological samples like serum/plasma or tissues (Merken and Beecher, 2000).

A. Principle

Basically all chromatographic systems consist of the stationary phase that may be solid,gel,liquid or a solid/liquid mixture that is immobilized and the mobile phase, which may be gas or liquid and it flows over or through the stationary phase. This partition is achieved in one of the following ways:

Adsorption chromatography : It is one of the oldest types of chromatography. It utilizes a mobile liquid or a gaseous phase that is adsorbed onto the surface of a stationary solid phase. The equilibrium between the mobile and the stationary phase accounts the separation of different solutes e.g. adsorption chromatography, hydrophobic interaction chromatography.

Partition chromatography : It is based on a thin film formed on the surface of a solid support by a liquid stationary phase. Solutes equilibrate between the mobile and the stationary liquid (e.g. partition chromatography, reverse phase chromatography, gas-liquid chromatography, etc.).

Chrysomelid beetle : Aulacophora foveicollis : ChrysomelidaeAphids : Macrosiphoniella  sanborni; Myzus  persicae; Brachicaudus  helichrysi; Aphis  gossypi; Aphis fabae; Aulocorthum circumflexum: Aphid

Chrysanthemum   is infested by different species of aphids. Aphids are small, soft bodied insects and their colour varies with species. Both nymphs and adults cluster on the growing stem tips and under surface of leaves. As a result, the plants show loss of vigor, distortion of leaves and flowers, yellowing, premature leaf fall and stunted growth. The damaged plants bear only undersized buds and they remain unopened. Peak damage period is November-December. Secretion of honeydew by the aphids favor development of sooty mould which hinders photosynthesis and the plants become stunted. They are also vectors of virus diseases.

Cinnamon butterfly: Chilasa clytia clytia : Papilionidae

Larvae defoliate plants. The larvae are pests on many crop plants and weeds and are common in the plains. The larvae are jet black at hatching. They develop a series of broad white patches. The adult is a common butterfly rich velvety brown, and spotted with white dots. The hind wings have arrow shaped streaks and crescent shaped spots.

Introduction
The development of the domestic phenotype mirrors the domestication process. Are certain behavioral patterns typically lost when animal are domesticated? Why do domesticated animals exhibit some behaviors more frequently than their wild counterparts? Darwin (1868) believed that in captivity biological traits are lost through disuse. Hemmer (1990) proposed that domestic animals have experienced a kind of regressive evolution, in which their behavior is less influence by environmental stimuli than that of their wild counterparts. The Earth’s rotation around its axis influences our physiology and behaviour. Animal species that are compelled to be active continuously typically exhibit poor performance, health, and survival. However, we examine the evidence of animals displaying extended periods of activity with weakened or nonexistent circadian rhythms and no observable
negative consequences. Available reports suggested that there is a greater prevalence of around-the-clock and ultradian activity patterns than commonly acknowledged.
This is especially true among herbivores, animals in Polar Regions and constant physical environments, animals in specific life-history stages, and highly social animals. Various mechanisms exist, but research indicates that certain circadian pacemakers still function in animals that are active throughout the day and night. The prevalence of continuous activity in various animals and habitats, along with a wide range of underlying mechanisms, suggests a pattern of convergent evolution. The circadian system’s organizational principles and complexity allow for potential chrono-biological plasticity. There could be compromises between the advantages of consistent daily patterns and adaptability, which, due to reasons that are not well comprehended, result in the functional suitability of irregular daily patterns only in specific environments and for specific ways of life.

Citrus butterfly: Papilio demoleus, P. polytes : Papilionidae

The pest is distributed all over the world and has a host range that includes citrus and other rutaceae plants like Aegle marmelos. Adult has the head and thorax black with a creamy-yellow coloration on the underside of the abdomen. Wings are dull black, orna-mented with yellow markings and a distinct red patch on the hind wing. The underside is orange and yellow coloured and can be seen when it flirts for honey collection or just before egg laying. The Antenna is black and clubbed.