eChapters

Fodder serves as the foundational element of livestock nutrition within natural farming frameworks. Providing animals with a consistent, nutritious, and chemical-free fodder supply throughout the year is essential to maintaining their health, optimizing productivity, and ensuring long-term sustainability. In contrast to commercial models that often depend on externally sourced feed concentrates, natural farming promotes the cultivation of fodder on-farm through organic and regenerative practices. India’s fodder supply is typically divided into the following categories:
• Green Fodder: This includes grasses like Napier, Guinea, and Dinanath, as well as legumes such as Berseem, Lucerne, and Cowpea, and nutrient-rich tree leaves from Moringa, Subabul, and Neem.
• Dry Fodder: Comprising crop residues like wheat straw, paddy straw, maize stalks, and groundnut haulms.
• Pasture and Forest Grazing: Utilization of common lands, silvopasture systems, and forest areas for grazing.
• Fodder Trees and Hedges: Protein-rich varieties like Gliricidia, Sesbania, and Leucaena, which also enhance soil fertility.
In natural farming, fodder crops are grown without the use of chemical fertilizers or pesticides. Farmers enrich soil and seeds with preparations like Jeevamrit and Beejamrit. Mulching helps conserve soil moisture, suppress weed growth, and foster microbial activity. Intercropping and crop rotation with cereals and legumes maintain ecological balance and soil vitality. Zero Budget Natural Farming (ZBNF) methods integrate livestock with crop cycles to regenerate fodder naturally.
The establishment of fodder banks and use of staggered sowing patterns ensures a steady supply even during off-seasons. In arid regions, drought-tolerant and native fodder varieties are prioritized to uphold fodder security.

Introduction

The concept of feeding system of different aquatic components of the aquaculture is not simple like that of domestic animals where feed, feeding and the animals can be observed closely and manipulated at once. In case of aquaculture both biotic and abiotic factors play an important role in feeding as well as the growth of the animal. Factors like water temperature, dissolved oxygen, light intensity, etc. regulate the intake of feeding, its digestion and assimilation. Conditioning factor, feed conversion rate, survival rate, percent weight gain, daily weight gain, specific growth rate are some of the parameters used routinely to monitor the effects of feeding on growth performance of the fish.

In aquaculture, supplementary feed is provided in conjunction with the available natural food such as plankton, periphyton, benthic organisms, aquatic plants etc. Aquaculture is a feed-based industry, with artificial feed accounting for major expenditure exceeding 60% of total operational cost. In India, freshwater aquaculture is mainly carp-based, which accounts for about 89% of total aquaculture production (Ayyappan and Jena, 1998). Successful and sustainable aquaculture depends upon the provision of nutritionally adequate, eco-friendly and economically viable artificial feeds. Effective utilization of feed depends upon many factors such as acceptability of the feed, its digestion, assimilation etc. apart from other rearing management protocols.

8.1. Introduction
Micronutrients are vitamins, minerals, and nutraceuticals that do not provide energy and do not raise any sensation of hunger known as “hidden hunger” or “insensible hunger”. Hidden hunger that causes micronutrient deficiency affects 2 billion people globally. Due to the deficiency of micronutrients, different diseases are noted among public health professionals that reflect poor community nutrition. To overcome these deficiency-related diseases, one of the important approaches is food fortification. Food fortification is a public health policy to reduce the number of individuals, who suffer from micronutrient deficiencies within a population. Common examples include iodine-tagged common salt, vitamin A-containing oil, and iron-enriched rice all of which are examples of food fortification. In the agricultural field, food fortification (FF) and/or food enrichment (FE) is the most efficient strategy to overcome various micronutrient deficiencies by the addition of essential micronutrients (vitamins and minerals) to enhance the nutritional value of cultivated crops. According to World Health Organization (WHO) and Food and Agricultural Organization (FAO) of the United Nations (UN), “Food fortification” is defined as “the practice of deliberately increasing the content of an essential micronutrient, i.e., vitamins and minerals (including trace elements) in a food irrespective of whether the nutrients were originally in the food before processing or not, so as to improve the nutritional quality of the food supply and to provide a public health benefit with minimum risk to health” (Pant and Chinwan, 2014). “Fortificant” refers to the incorporation of substances in the food to provide micronutrients. “Micronutrients” refers to the important dietary components, including vitamins and minerals, that are required in very small amounts but are engaged in very critical roles in various body functions and disease prevention.

Food is defined as any substance, whether processed, semi processed or raw, that is intended for human consumption as well as the inedible parts associated with food that are not intended to be consumed by humans. Food waste is defined as any food and inedible parts of food, removed from the food supply chain that can be recovered or disposed, whereas, food loss is defined as food that unintentionally undergoes deterioration in quality or quantity as a result of food spills, spoils, bruising, wilting or other damages alike owing to infrastructure limitations at the production, storage, processing and distribution stages of food lifecycle. Global food waste management is an issue of concern that requires attention from various stakeholders having diverse expertise. According to statistics, about one third of the food produced for human consumption is either lost or wasted. Globally, the cost of food waste accounts for USD 2.6 trillion, of that USD 1trillion is incurred from greenhouse gas (GHG) emissions, water scarcity, biodiversity loss, increased conflicts and loss of livelihood owing to issues like soil erosion, nutrient loss, reduced yields, wind erosion and pesticide exposure.

A hen can produce eggs without mating by a cock. Such an egg is edible but infertile and will not hatch. If the hen is naturally or artificially inseminated with poultry semen, the spermatozoon unites with the ovum to form a fertile egg that will hatch after incubation. Unlike mammals, the embryo of birds grows independently within an enclosed hard structure called shell and thus, all nutrients required for embryonic development are contained in the egg. The yolk, albumen, shell membrane and shell protect the developing embryo apart from providing well balanced nutrients.

Introduction

In developing countries, ruminants thrive mainly on poor quality feeds and pastures, whose nutritive value is poor. They are having a low content of nitrogen, energy, minerals and vitamins and high content of lignin and silica, which limit their efficient utilization by ruminants. Therefore, these poor quality feeds and fodder needs to be upgraded in terms of their nutritive value using various treatments like physical, chemical, physico chemical and biological methods to improve intake and utilization of nutrients from poor quality roughages. However, supplementation of critical nutrients like energy, protein, minerals, vitamins also improved the productivity and health of the ruminants (Dass et al., 2011). Availability of oil cakes as protein supplement in developing countries is very poor and at a very high price, This has led to use of non-protein nitrogen source as urea, to compensate for the nitrogen deficiency of fibrous feeds (Dutta et al., 2009). Thus, enhancing intake, digestibility and availability of nutrients from poor quality roughages are only possible through optimization of rumen fermentation. The use of solid multi-nutrient block supplementation /urea-molasses blocks/ multi nutrients blocks/ pashu chocolates to provide optimum levels of various nutrients primarily for better growth of rumen micro organisms. They need rich sources of fermentable nitrogen, minerals and vitamins, which are lacking in the fibrous feeds like cereal straws (Sahoo et al, 2009). This offer several advantages such as easy transport, storage and handling, and reduced risks of poisoning as compared with other approaches, such as giving a small amount of urea in drinking water, sprinkling of urea solution on fibrous feeds before feeding, or urea-ammonization of crop residues (Dutta et al ., 2009).

A premixisa mixtureofvitamins, trace minerals, medicaments, feedsupplements and diluents either individually or in a combination. It is a value added solution for feeds with sustainable safety and quality.

The main objective of premixes is to deliver the micro-ingredients in a manner desired by customer. These blends of Micro-ingredients need to be carefully selected, analysed and homogenously manufactured in controlled conditions.

Good quality premixes are manufactured as per following four fundamental principles that determine premix quality and performance :

1. Understanding the chemical and physical attributes in the microingredient to be incorporated.

2. Testing to ensure that all microingredients meet specifications.

3. Controllingthe inclusionin fullproof and secure method.

4. Blending together in speciality mixers to achieve a homogenous mix.

The formulation of ration for various categories of livestock depends upon the nutrient requirements for a particular animal or group of animals and the feeds and fodders available for the purpose. Livestock feeds can be grouped into two major categories – Roughages and concentrates, on the basis of bulkiness and chemical composition and combination of both is essential for a balanced ration.

Roughages are the bulky feeds having crude fiber more than 18% and TDN less than 60% while, Concentrates are feed or feed mixture having crude fiber less than 18% and TDN more than 60%. They are rich in either energy or protein and accordingly are called as energy concentrates (when CP is less than 18%) or protein concentrates (when CP value exceeds 18%). Various energy concentrates are grains and seeds (maize, barley, sorghum etc.), milling byproducts (brans and chunis), roots (tapioca tubers, turnip, potatoes) and molasses.

Introduction

As India is the leading global economy with livestock contributing 4.66% in total GVA to Indian Economy (PIB, 2024) and ranks 1st in Milk Production, 2nd in Egg production, 5th in meat production (BAHS, 2024). Being the largest exporter of milk and meat in the world , this has led to the intensification of animal production due to the increasing demand of major livestock products (MLPs), which has led to an increasing overall use of antimicrobials. Antimicrobials are invariably used to preserve animal and public health, but also as growth promoters at a sub-therapeutic level. The overuse and misuse of antibiotics can contribute to antimicrobial resistance. The growing resistance to antimicrobial drugs could reverse the therapeutic benefits; animals’ resistance to antimicrobial drugs, reduces productivity and leads to economic losses. Unwanted antimicrobials residues may be present in products of animal origins, in animal waste contaminating soil and water and the environment. Seventy-five to ninety percent of antimicrobials used in livestock are excreted, mostly unmetabolized. AMR poses a serious threat to the safety and quality of animal origin food, food security and livelihoods. Unhealthy and unproductive animals are no longer able to generate food products of acceptable safety and quality for human consumption and can no longer contribute to income generation. Insufficient standards for residues in animal food products, reduces the livestock sectors potential of access to trade and increases public health risks (https://www.fao.org/antimicrobial-resistance/key-sectors/animal-production/ en/).

Chromatography is one of the most effective technique for accurate, fast and convenient separation and identification of the components of a mixture. The method was developed by Russian botanist Mikhail Tswett (1906), described by Karl Runge (1855). Martin and Synage (1944) received Noble Prize for developing methodology of partition Chromatography.

Partition chromatography

Partition chromatogaphy is a system comprising of two physically distinguished components - a mobile component or phase and a stationary component or phase. A mixture of substances which are to be separated is placed on the system and separation takes place on the basis of difference in distribution of molecular species in two phases. Relative movement is a result of driving force and retarding effects.

Stationary phase is the sorbent, which may be liquid or paper or gel. If it is liquid then it is held stationary by adsorbing on a solid support or matrix. The mobile phase may be solvent or developer or a gas. Since the two phases are immiscible, the partitioning is defined as the distribution of solute in two immisicible phases which are in contact with one another. When a column is filled with the sorbent and solvent the two phases form a large number of theoretical plates. Each plate contains both the phases. Solutes in solution (mixture of substances to be separated) is layered on the top of the column and when it enters to the column it distributes in the two phases. The ratio of the concentration of a solute in two phases is called its partition coefficient. The resolution of solutes improves as the length of stationary phase increases.

Two procedure are described for the determination of free gosssypol . The first for normal meals and the second for meals which have been chemically treated and so contain dianilinogossypol.

Reagents
 
    1.    Aqueous acetone:  7 parts acetone, 3 parts distilled water (V/V).
    2.    Aqueous acetone-aniline solution: To 700 ml acetone and 300 ml distilled water, add 0.5 ml redistilled aniline. Prepare solution daily.
    3.    Aqueous isopropyl alcohol solution: 8 parts isopropyl alcohol. 2 parts distilled water (V/V).
    4.    Aniline Distill reagent grade aniline over a small quantity of zinc, dust discarding the first and last 10 percent of the distillate. Store refrigerated in a brown glass stoppered bottle. Solution is stable for several months.

The cotton aphid :Aphis gossypii: Aphididae

Both nymphs and adults cause the damage by sucking the plant sap from shoot tips, flowers and leaves.  Severe infestation may result in complete drying of affected parts.  Attacked leaves curl up, wither and fall. These are yellowish green or brown coloured aphids.  The total life cycle from first instar nymph to adult occupies an average of 8-10days.  It reproduces parthenogenetically and viviparously.  The female may produce 10-30 young ones in a life span of 10-12 days.  The nymphs transform into adult in 5-8 days after passing through four moults.

Introduction

The science of Biotechnology has been making every effort to create new opportunities for the welfare of humanity. Modern biotechnological tools are applied in India to boost fish production through aquaculture as well as conservation of the vast aquatic resources of the country for sustainable utilization. Transgenic technology is widely used in biotechnology, from the generation of genetically modified (GM) foods to the production of pharmaceutical proteins. This technology has been successfully used to develop fast growing super-fish stocks for aquaculture. The main reasons for genetic manipulation of species used in aquaculture are to enhance the growth and efficiency of food conservation, to increase tolerance to environmental variables like temperature and salinity, to produce new colour variants of ornamental fishes, to increase the resistance of species to pathogens, etc. Though the conventional selective breeding approach has proven to genetically upgrade rohu19, it is a rather time consuming process. Traditional selective breeding approaches in combination with transgenesis can bring about faster improvement of selected traits.

These include mainly tomato pomace, jack fruit waste, pineapple waste, apple pomace and citrus fruit processing waste.
1. Tomato pomace (Lycopersicon esculentum): Tomato pomace consists of the remnants of tomato processing, including peels, crushed seeds, and minimal pulp, which are left after extracting juice, paste, or ketchup. Following juice extraction, the pomace is typically dried and ground. Due to its high moisture content, artificial drying methods can be costly, making sun drying a more favorable option. The pomace should be dried until it reaches a crisp texture. After drying, it is essential to grind the pomace and incorporate the resulting product thoroughly into the diet. The composition of tomato pomace includes 93.5% DM, 17-22% CP, 39% CF, 11.1% EE, 0.44% Ca and 0.36% P, with 55% TDN. In terms of dietary inclusion, tomato pomace can be incorporated into the rations of adult bullocks and milch cows at levels of up to 50% and 16%, respectively, without negative effects. In dairy cows, the intake of dry matter, milk yield and milk composition remained unaffected. Additionally, fresh tomato pomace can serve as a supplementary feed for growing and finishing pigs. Notably, feeding growing pigs a diet containing 6% fresh tomato pomace resulted in a significant increase in feed consumption compared to those receiving a standard commercial mash.
2. Citrus by-products: Citrus fruits encompass several significant varieties, with the most notable being the orange (Citrus sinensis), lemon (Citrus limon) and grapefruit (Citrus paradisi). The by-products derived from citrus processing are affected by various factors, including the fruit’s origin and the method of processing employed. Consequently, the nutritional value of these by-products varies based on the type of waste and the specific fruit involved. The citrus by-products contain 1.7-7% TA, 1.2-2.1% EE, 5.7-8.6% CF, 2.2-4.2% CP, 65-75% NFE,
10.2-16.5% total sugar, 1.3-6.5% lignin and 4.4-12.8% pectin. Fresh citrus pulp waste is considered palatable for cattle, with a consumption capacity of up to 10 kg per day. Dried citrus pulp can be incorporated into animal feed at levels as high as 45% of the total ration. However, it is important to note that the digestibility of this feed in sheep diminishes when the inclusion exceeds 30%.

Introduction

Nutrients are essential substances present in different types of foods which perform various life-sustaining functions in the body. They are required for growth, maintenance and the reproductive process. Poultry require more than 40 such nutrients, which are classified into six major groups - water, carbohydrates, protein (amino acids), fats, minerals and vitamins. Poultry diets must be formulated to provide all these essential nutrients as per the bird’s requirement to achieve optimum growth and production. Several ingredients including cereal grains, cereal by-products, fats, plant protein sources, animal by-products, vitamin and mineral supplements, crystalline amino acids and feed additives are commonly used to prepare poultry diets. Further, specialist areas such as immuno-nutrition are rapidly gaining attention due to continued advancement in the field of poultry nutrition (Beski et al., 2015).

This chapter introduces the functional properties that make eggs valuable ingredients in food systems. It explains coagulation and heat-setting, foaming and aeration, emulsification, gelation, colour and flavour development, binding, adhesion and thickening. The effects of pH, temperature, processing conditions and other factors on functional performance are examined. Applications in bakery, confectionery, mayonnaise, sauces, meat products and other foods are discussed. The chapter demonstrates that eggs provide multiple technological functions simultaneously, making them particularly valuable and difficult to replace with a single alternative ingredient.

Every nutrient has its specific functions to play in the biological system. As we know there are six nutrients i.e., water, carbohydrate, protein, fat, minerals and vitamins and each of these have different functions, it will be discussed separately for each nutrients.

2.1 Water

Water is the ideal dispersing medium because of its solvent and ionizing powers which facilititates cell reaction and due to its high specific heat which helps to absorb the heat of these reactions with a minimum rise of temperature. Lean adult body contains about 70 percent of water, though the amount varies from embryo to mature animals.

Artificial intelligence offers practical solutions to agricultural problems associated with population growth, food demand, climate change and limited natural resources. This chapter introduces machine learning, deep learning, computer vision and natural language processing, together with enabling technologies such as IoT, remote sensing and cloud computing. It explains their applications in crop monitoring, disease recognition, yield forecasting, irrigation, soil assessment and livestock management. AI can improve decision-making, resource efficiency, automation and precision farming while reducing reliance on manual labour. The chapter also examines adoption barriers, including high costs, limited digital literacy, data-security concerns and weak rural infrastructure, and considers future developments in autonomous and climate-resilient farming.

Fungus is member of group of eukaryotic organisms that includes yeasts and molds. The fungi are heterotrophs and they acquire their food by absorbing dissolved molecules by secreting digestive enzyme in the environment. It is present in common place in the environment and some are normal inhabitants of the skin, gastrointestinal tract and other mucous membrane surfaces. Generally if birds immunity is intact and fully operational then it will overcome the fungal infection in most situations, In case of compromised immune system it will cause development of serious infections. Paramount to properly managing fungal infections in avian species is the ability to recognize infection early in the course of disease, to administer appropriate antifungal medications for the location and severity of infection, and to continually assess a patient’s response to therapy.

1. What is a characteristic feature of fungal cell walls?

a) Peptidoglycans

b) Ergosterol and polysaccharides

c) Antiphagocytic capsules

d) Fruiting bodies

2. How do fungi reproduce in tissues?

a) Binary fission

b) Budding

c) Simple division of round, yeast-like forms or slender, tubular hyphae

d) Spore formation