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In the existing planetary system, life was established and growing over millions of years on earth. If we track down to the history of evolution, beginning with a living unicellular organisms Amoeba, growing to a gigantic size of Dinosaur and stabilizing at well adaptable stage of an Elephant in animal kingdom or to reach the present status of the intelligent human being, throughout these developmental stages the climatic/meteorological factors play a crucial and supportive role. Further the Development of plant/Animal kingdom is making rapid strides in tropical climatic zones where the temperature ambient ranges between 20⁰C to 40⁰C and the rain fall/humidity factors are moderate. We are fortunate enough that India is geographically located in tropical zone. This classic location advantage of the country in the world geographical map helped the country in keeping India as number one milk producer in the world, no 3 producer in poultry egg production, number 5 in horticulture production i.e fruits and vegetables. Climate change affects livestock both directly and indirectly. Direct effects from temperature, humidity, rainfall, wind speed and other climate factors, aggravates the heat stress in dairy animals, and influences animal performance badly: in growth factors, milk production in Dairy animals, mutton production/ wool production in case of sheep. egg and meat production in poultry, and reproductive aspects in all the species. The indirect impact of these factors on livestock may result on economic, nutritional and health aspects of human society and also its contribution to the economy of the country.

Introduction

Poultry production is one of the fastest growing sectors of livestock industry in developing countries. Environmental variation is one of the major factors that affect sustainability of poultry production systems in tropical climate (Sinha et al., 2017a). Adverse climatic stress declines the production performance of chicken which causes high economic loss to the enterprises as well as the farmers. Heat stress has an adverse effect on egg production, egg weight and shell quality of laying hen (Whitehead et al., 1998). Climatic variables like temperature, humidity, radiation and wind speed that directly affect the mechanism of thermoregulation and rates of heat exchange by all animals (NRC, 2001). Heat stress is a major factor that decreases productivity and reproductive efficiency of livestock due to lower feed intake and negative energy balance (De Rensis and Scaramuzzi, 2003). Moreover, it badly affect the production due to decline feed digestibility such as proteins, fats, starch (Bonnet et al., 1997). In addition, the acute heat stress drastically decreases the reproductive performance of hens due to alterations in acid-base balance and ion exchange mechanism (Mahmoud et al., 1996). Climate change in?uences the emergence of disease and their transmission due to increases vectors, pathogens. All the above effects impact a huge economic loss to the farmers as well as the country.

Agriculture and allied sectors have adversely hit by the COVID-19 scare.The rumors of poultry birds likely the carrier of the virus circulated in social media had led to reduced demand of the chicken meat in several parts of the country in the month of February 2020 even before India reported the first case of COVID-19. The poultry industry in India hit hard because of nationwide lockdown to stop the rapid spread of COVID-19 virus. This lock-down had disrupted the entire supply chain. Before COVID-19 the prices for broiler was about Rs. 80 per kg and Rs. 4 for egg the price dropped to almost no value like Rs. 6 per kg and Rs. 1-1.50 per egg. As a result, the projected loss of the industry was about Rs 25000 crore. (Shukla & Bhattacharyya, 2020).
According to industry sources, the poultry industry which had witnessed steady growth rate in the last two decades because of rising consumer demand for protein rich food especially but, it has incurred huge losses since the beginning of the 2020. The loss incurred by Indian poultry industry is mainly attributed to sharp decline in demand because of disruption of supply chain during lock down. The financial condition of all the stakeholders in the poultry value chain – farmers, feed suppliers and retailers had become risky. The drop in demand and supply chain disruption especially hit the smaller farmers who had limited
resources. Thus, many farmers went for ‘contract farming’ offered by many large poultry players.

Introduction

The process of making the rumen of animals free of rumen protozoa is called defaunation and the animal is called defaunated animal. The role of rumen ciliate protozoa on the performance of host animals became debatable issue when Becker and Everett (1930) demonstrated that rumen protozoa were non-essential for growth in lambs. Nevertheless, the reports of recent years reflect that though protozoa may be non essential for ruminant, still they have significant role to play in the rumen metabolism specially to stabilize the rumen pH (Santra and Karim, 2002a). Rumen protozoa are the largest in size among rumen microbes and contribute 40-50% of the total microbial biomass and enzyme activities in the rumen (Agarwal et al., 1991).

Introduction

Sheep and goats have a vital economic importance in India, inhabitant in semi-arid region and mostly rose under harsh environmental conditions (high ambient temperature, scarcity of feed and water). The arid and semi-arid regions of India are drought prone due to low and uneven rainfall.  The vegetation of arid and semi-arid tract is more acute during summer when the quality of the pastures becomes vulnerable which in turn decreases the body condition score of animals and reproductive efficiency is being compromised (Maurya et al. 2009a & 2010).  The small ruminants are widely distributed in comparison of other domesticated species. These species of livestock is also a source of employment for marginal and land-less farmers particularly in the arid and semi-arid region of India. Most of the Indian farmers have limited resources and small ruminants production offers an opportunity to alleviate financial conditions and improve living standards.

Livestock production plays a critical role in global food security and economic sustainability. However, various stressors can significantly impact the well-being and productivity of livestock animals. Stress in livestock can arise from various sources such as environmental conditions, management practices, transportation, social interactions, and disease. Understanding the impact of stress on livestock production is essential for ensuring animal welfare and optimizing productivity. Although many factors can be involved, climatic factors are among the first and crucial limiting factors of the development of animal production in warm regions. In addition, global warming will further accentuate heat stress related problems in livestock. The first and foremost stress the livestock are exposed as a result of climate change is the heat stress. Among the environmental variables affecting animals, heat stress seems to be one of the intriguing factors making animal production challenging in many geographical locations in the world. Although animals can adapt to the hot climate, nevertheless the response mechanisms that ensures survival are also detrimental to performance. The vulnerability

Impaction of the alimentary tract is a condition where ingesta or foreign material accumulates excessively in one or more segments of the gastrointestinal system, causing a blockage. In ruminants, the rumen and reticulum are common sites of impaction due to their roles as primary fermentation chambers. This condition significantly impacts the animal’s health, productivity, and welfare, and if left untreated, it can lead to severe complications or death.
Pathophysiology of impaction
Impaction occurs when the normal passage of ingesta is hindered due to excessive accumulation of indigestible, poorly digestible, or foreign materials.
This leads to:
1. Decreased motility: Overloading the rumen or reticulum impairs their motility, disrupting the normal mixing and fermentation processes.
2. Reduced absorption: The accumulation hinders the absorption of volatile fatty acids (VFAs) and other nutrients.
3. Fermentation imbalance: The retention of ingesta alters microbial populations, causing reduced fermentation efficiency.
4. Secondary issues: Prolonged impaction may lead to dehydration, metabolic acidosis, rumenitis, and systemic effects like toxemia.

Introduction

Growth enhancement technologies have been widely embraced in the livestock industry to improve growth, efficiency and carcass traits. For over 50 years, estrogenic and androgenic hormone implants were widely used in the cattle feeding industry, and it is estimated that approximately 97 percent of feedlot cattle in the U.S. receive one or more implants during the finishing phase (Barham et al., 2003; Tatum, 2006). There is a large body of literature that indicates the positive growth enhancement effects which implants impart on growing and finishing cattle and producers have extensively used implants to increase live body weight, improve average daily gain and feed efficiency, and reduce the number of days cattle are on feed (Apple et al., 1991; Duckett et al., 1997;Milton and Horton, 1996; Perry et al., 1991). Likewise, there is a growing body of literature that indicates the positive response on growth and carcass characteristics that finishing cattle have to beta-adrenergic agonists. The hog industry, and more recently, the cattle feeding industry have increased utilization of beta-adrenergic agonists to improve the efficiency of production. While the use of Ractopamine hydrochloride (Paylean^®, Elanco Animal Health, Greenfield, Indiana) has been used extensively in hog production since its approval by the Food and Drug Administration (FDA) in 1999, similar pharmaceutical feed additives have only recently been implemented in commercial cattle feeding operations. Beta-adrenergic agonist use in fed cattle has substantially increased following the FDA approval of Ractopamine hydrochloride (Optaflexx^®, Elanco Animal Health, Greenfield, Indiana) in 2003, and the approval of Zilpaterol hydrochloride (Zilmax^®, Merck Animal Health, Summit, New Jersey) in 2006. The use of both Ractopamine hydrochloride (RH) and Zilpaterol hydrochloride (ZH) have shown to elicit similar improvements in live weight gain, average daily gain, and feed efficiency as those reported in hormone implants (Allen et al., 2009; Avendano-Reyes et al., 2006; Beckett et al., 2009; Elam et al., 2009; Gruber et al., 2007; Kellermeier et al., 2009; Scramlin et al., 2010). In addition, these compounds have been reported to have profound effects on hot carcass weight, Longissimus muscle area and carcass cutability (Gruber et al., 2007; Hilton et al., 2010; Rathmann et al., 2009; Scramlin et al., 2010; Shook et al., 2009; Vogel et al., 2009). It is because of this that cattle feeders have drastically increased use of beta-agonists since their FDA approval, and a growing portion of the fed cattle population receive a dietary beta-agonist supplement that is provided within the final 20 to 30 days of finishing.

The profitability of dairy animals depends on milk yield and reproductive efficiency. High-yielding cows require more nutrients to support increased milk production. However, early lactating cows and buffaloes often face a Negative Energy Balance (NEB) due to inadequate energy intake, which hampers their performance. To enhance productivity, it is essential to minimize the extent and duration of NEB. One effective approach is the use of bypass nutrients, which safeguard essential dietary components like fat and protein from rumen fermentation, enabling better digestion and absorption in the lower digestive tract. Additionally, protected starch, chelated minerals, and vitamins can also boost animal performance.
“Bypass Nutrients” are nutrients that undergo minimal fermentation in the rumen, allowing them to remain intact for digestion and absorption in the lower gastrointestinal tract. These slowly degradable nutrients provide a steady nutrient supply to rumen microbes, unlike easily soluble nutrients that lead to sudden spikes in nutrient availability.
Rationale behind the protection of dietary nutrients
The potential benefits of delivering nutrients directly to the intestine in a form available for direct absorption and utilization by the animals are:
1. Prevention of energy losses associated with the degradation and fermentation of nutrients in the rumen, thus sparing available energy for productive purposes.
2. Ensuring that particular nutrients will be available to the host animal in their original form.
3. Taste masking of unpalatable substances.
4. Preventing interactions among nutrients and their oxidation during storage.

Livestock production is an integral part of agriculture and plays a significant economic role in most developing countries. It contributes to poverty alleviation, food security and provides elements that are essential to the national economy. Livestock production accounts for 40% of the gross value of the agricultural production globally and this figure is likely to go up, as the demand of livestock products is increasing rapidly with the increase in income and urbanization (FAO, 2012). For poor farmers, animal ownership ensures varying degrees of sustainable development and economic stability. Livestock provide a stable cash reserve independent of inflation and are an important source of traction. Livestock are closely linked to the social and cultural lives of several million people. Quality and quantity of feed is essential for optimum animal productivity. Inadequacy of quality animal feed resources is most often the crucial factor for livestock production. The success of livestock is greatly dependent on continuous supply of good quality nutritious feed at competitive price. Poor nutrition of animals has been identified as the major constraints to animal production across the developing world (FAO, 2000). Feed is the major input cost in animal production system, accounting for 65-70% of the total rearing cost.

There are 6 nutrients which are very important to animal body – carbohydrates, proteins, minerals, vitamins and water.
Carbohydrates: These are polyhydroxy aldehyde or ketone as in monosaccharide or polymers in oligo or polysaccharides. The question arises whether carbohydrate is a dietary essential !!! Carbohydrate may not be dietary essential, but it is definitely metabolically essentially. The organic compounds – fats, proteins are oxidized only in the presence of carbohydrates. Carbohydrates are the structural components of DNA, RNA, and some other vital organic molecules. Although less than 1% is present in the human body [because, constantly being formed and broken down in metabolism], yet without the presence of this carbohydrate, the existence of living creature is at stake. Carbohydrate in the form of glucose primarily provides energy in the body and excess amount of carbohydrate is stored as glycogen in the liver and muscle. More than 50 % of the energy value of the diet is provided by carbohydrate.
Glucose + 6 O2 6 CO2 + 6 H20 Δ E (675 k. cal).
Lipids: These are important constituents of plant and animal tissue and these can be extractable from biological materials with the usual fat solubles, for example – Ether, Chloroform, Benzene, Carbon tetrachloride, Acetone etcetra. The lipid metabolism is in dynamic state. There is constant mobilization and transportation of fatty acids from the depots. Some portion of absorbed fatty acids are degraded in the same way, while others are combined with glycerol transported back to depots. All these reactions are so balanced, that mixtures of fatty acids in the depots, blood and organs tend to remain at equilibrium
condition.

There are six nutrients which are very important to animal body and they are – Carbohydrates, Lipids, Proteins, Minerals, Vitamins and water.

Carbohydrates: These are polyhydroxy aldehyde or ketone as in monosaccharide or polymers in oligo or polysaccharides. The question arises whether carbohydrate is a dietaryessential !!! Carbohydratemay not be dietary essential but it is definitely metabolically essential. The organic compounds – fats, proteins are oxidized only in the presence of carbohydrates. Carbohydrates are the structural components of DNA, RNA, and some other vital organic molecules. Although less than 1% is present in the human body (because constantly being formed and broken down in metabolism), yet without the presence of this carbohydrate, the existence of living creature is at stake.

Carbohydrate in the form of glucose primarily provides energy in the body and excess amount of carbohydrate is stored as glycogen in the liver and muscle. More than fifty per cent of the energy value of the diet is provided by carbohydrate.

Glucose + 6O₂ → 6 CO₂ + 6H₂O + Δ E (675 k.cal)

They also exhibit protein sparing action because proteins are mainly required for tissue-building i.e. general wear and tear in the body. If there is any emergency, say animal is deficient in calories of the diet, then it will use adipose and protein tissues. It is said that proteins and fats are burnt or oxidized in the flame of carbohydrate.It means thatcertain intermediarycompounds of glucose oxidation through krebscycle are absolutely necessary for oxidation of proteins and fats. Again, in any emergency, if glucose level of the body goes down, fats and proteins take over and they get metabolized faster than the animal body

A. Answer the following questions:

1.    What is Basis of scientific feeding?

Ans: Knowledge of the quantitative needs of the body for various nutrients and the relative value of feeds as sources of them is the basis of scientific feeding.

2.    What is the minimum requirement of animal per group to conduct a feeding trial?

Ans: Minimumanimal per group should be 4.

3.    When you will go for t test and ANOVA test?

Ans: In case of two rations, ‘t’ test and if it is 3 or more then ‘Analysis of Variance’ or ‘ANOVA’ test is applied.

4.    What are the advantages of feeding trials with laboratory animals?

Ans: It involves much less cost of animals, less feed and labour cost,requiredlesstime,individualvariabilitycanbe minimized due to similar genetic and nutritional history which is not possible in case of large animals, large number of animals can be used at a time and easy to slaughter the animals for histological and chemical analysis

5.    What is the purified form of nutrients?

Ans: Casein, urea or purified soybean protein for protein; starch, glucose or sucrose for carbohydrate; oils or lards as source of fat; chemically pure salts as minerals; pure crystalline compounds as vitamins.

Fluids and electrolytes are critical in managing dehydration, electrolyte imbalances, and shock in animals. These life-saving interventions restore homeostasis in various disease conditions. Fluid therapy is tailored based on species, body weight, disease condition, and hydration status, ensuring proper restoration of blood volume, electrolyte balance, and acid-base homeostasis. Principles of fluid therapy
1. Types of fluids
Crystalloids: Normal saline, Ringer’s solution, and lactated Ringer’s solution (LRS). Colloids: Plasma, hydroxyethyl starches, and dextrans. Dextrose solutions: For energy supply and hypoglycemia.
2. Routes of administration:
Intravenous (IV): For severe dehydration and shock.

non-herbaceous plants that are accessible for animal foraging.
Flour: It is soft, finely ground meal of a grain which mainly consists of gluten and starch.
Fodder: Fodder covers various agricultural products specifically intended for the nourishment of domesticated animals. The term “fodder” is particularly associated with the provision of food to these animals, which may include plants that are harvested and delivered to them, as opposed to the food they gather independently through foraging. Any green plant or crop meant for animal feeding for supply of nutrients and bulk.
Forage crop: A crop of cultivated vegetation or portions of plants, excluding detached grains, that is grown specifically for grazing or harvesting to serve as animal feed. Forage and roughage these two terms are used interchangeably. Forages include hay, straw, silage and pasture while roughage include rice husk, groundnut shells.
Forage crops: It denotes the wild as well as cultivated green plants used for feeding of domestic animals like cattle, buffaloes, goat, sheep, horses, etc.
Forage: Forage includes consumable plant materials, including grasses, legumes, herbs and shrubs, as well as hays and silages. These materials are either grazed or ingested by livestock to meet their nutritional needs and dietary requirements within agricultural ecosystems.
Germ: It is the embryo of any seed. eg. Wheat germ meal (25% CP and 7% EE)
Gluten: When flour is washed to remove starch, a tough, viscid nitrogenous substance remains which is known as gluten. eg. Corn gluten.
Herbage: Herbage denotes the various plants, grasses, or vegetation that are specifically designated for the nourishment of livestock. This term generally encompasses the softer, green portions of plants that are appropriate for animal grazing.

Vitamins and minerals play crucial roles in supporting immune function across various animal species. Their supplementation can enhance immune responses, reduce disease susceptibility, and improve overall health. The appropriate doses vary depending on species, physiological state, and environmental conditions.
Cattle
1. Vitamins
Vitamin A: Enhances mucosal immunity; 30,000–50,000 IU/day.
Vitamin D: Supports macrophage function; 10,000–15,000 IU/day.
Vitamin E: Antioxidant, improves phagocyte function; 500–1,000 IU/
day.
2. Minerals
Zinc: Promotes wound healing and immune cell activity; 40–60 mg/kg feed.
Enhances neutrophil and antibody responses; 0.3 mg/kg feed.
Sheep and Goats
1. Vitamins
Vitamin A: 5,000–10,000 IU/day.
Vitamin E: 200–400 IU/day to enhance antioxidant defenses.
Vitamin D: 2,000–3,000 IU/day to maintain calcium balance and immunity.

Desi chickens reared under backyard systems face several constraints such as lower productivity, higher incidence of disease outbreaks, high mortality rates during the early stages of life, and predation. Despite these challenges, the demand for rural backyard poultry is high, particularly among tribal households in rural areas. Small rural producers typically rear colored birds and produce brown-shelled eggs from Desi backyard poultry, catering to the requirements of rural consumers.

To address these issues, there is a need for specific rural poultry production programs. The Indian Council of Agricultural Research (ICAR), New Delhi, initiated a project to upgrade indigenous low input technology birds through the All India Coordinated Research Project on Poultry (AICRPP) in various parts of the country. This initiative aims to evolve improved rural backyard poultry breeds.

Mostly goats are reared in extensive small holder systems in developing countries. Goat breeding programmes in these environments have been limited, amongst other factors, by the non availability of pedigree and performance records for traits of economic importance. Cross-breeding and selection play an important role in increasing production in the goats.The development of breeding strategy for the improvement of goats should be based on genetic parameter estimates of economically important traits. Biological, cultural, statistical, socio-economical and management aspects should also be an important component of the selection process.

Goats were domesticated for the triple purpose of milk, meat and hair. Although the development of milk and hair producing animals did not go together, the development of meat and hair together worked very well and there are several breeds like Angora which in addition to providing fiber, are excellent meat animals too. The development of meat breeds was favored by natural selection. The characters which aided to this aspect were high fertility rates and the relatively more active life which goats were forced to lead in order to get enough of nutrition in poor agricultural lands, deserts etc. where these animals were domesticated. These animals were exposed to the vagaries of the environment resulting in decline in production traits such as milk, meat etc. But meat production was not. Most of the breeds of Asia and Africa are thus predominant meat breeds which have been improved little by conscious effort of breeders. Some of them still have slow growth rate and feed conversion efficiency. The preference for goat meat had eroded the dairy potential of Indian goats, as the fastest growing males are slaughtered for meat purposes and only poor animals are retained for breeding purposes. Careful selection and utilisation of breeding males can alleviate this problem to a large measure and animals for both milk and meat production can be developed.There are several goat breeds that have good potential for meat production. Generally, there have been limited planned selection and breeding programs carried out by animal scientists to improve rate of growth and carcass composition of goats. As a result, many goat breeds deposit less intramuscular and subcutaneous fat. In the past, domesticated meat animals were selected for heavier mature weight and greater rate of gain. Selection for such parameters usually resulted in undesirable characteristics because some of the animals had not only gained weight faster and were heavier at maturity, but also had more subcutaneous and intramascular fat. Since consumers currently prefer leaner red meat, the livestock industry is making significant changes to satisfy these demands. Goat meat has unique attributes which will be beneficial to the present day consumer. Goat meat has 10% more lean carcass than cattle. At the same time the fat content is lower by 47% than beef. These qualities of the goat meat need to be emphasized so that the general public can become aware of this useful product.

Introduction

It is a well known fact that the soluble oxalates are broken down by rumen microbes from buffalo and cattle and one of the end product formed is bicarbonates.

The rumen microbes are obtained from a fistulated buffalo calf fed on wheat bhusa and sarson cake supplemented with 60 g potassium oxalate daily for about three months. Representative sample of rumen ingesta is drawn before feeding and mixed culture is prepared by the method as described Donefer et al., (1960). Mixed culture and basal nutrient mixture (Quicke et al., 1959) of pH 6.9 mixed in equal quantities. Fifty millilitres of this mixture are added into each incubation flask containing one gram of wheat straw, ground to 40 mesh as substrate. In the incubation flasks are added 35,100 and 550 mg oxalic acid in the form of potassium oxalate and 0.24 µCi sodium oxalate 14C. Blank (containing no substrate, no oxalic acid added) and two controls, one having substrate but no added oxalic acid and the other containing the above plus radioactivity, are also run alongwith. All the incubation flasks are taken in duplicate. The flasks are incubated at 39ºC for 24 h  in an incubator and at the end of this period microbial activity is stopped by the addition of few drops of saturated solution of mercuric chloride.

Incubation is a critical process in poultry farming, involving the hatching of fertile eggs to produce chicks. This process can be carried out by natural or artificial means. Natural incubation involves placing eggs under a broody hen or duck, allowing the mother bird to provide the necessary warmth and conditions for hatching. In contrast, artificial incubation uses machines called incubators to create the ideal environment for the eggs to hatch. The incubation period, which varies among different poultry species, remains consistent within the same species regardless of whether natural or artificial methods are used.

Incubation

• It is simply the production of chicks by hatching fertile eggs either by natural or artificial means.