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As the heart diseases in companion animals is becoming common as in humans, the research in canine cardiac diseases is one of the most extensive studies in the present scenario. As per the available literature, the overall occurrence of various cardiac abnormalities ranges from 0.37 percent to 1.67 percent in various parts of the country. Whereas, in western parts of the globe, nearly 8 million dogs suffer from heart disease accounting 10% of all dogs in the United States. Heart diseases are common among older dogs; up to 75% of senior dogs have some type of heart condition, and unfortunately, most go undiagnosed due to varied reasons. The recognition of canine cardiac diseases has been delayed, and ignored on account of lack of awareness and knowledge by the owner and inadequate diagnostic facility in our country. Physical examination, radiography, electrocardiography and echocardiography are most useful diagnostic procedures employed in diagnosing the cardiac diseases in dogs. However, majority of the acquired cardiac diseases are being managed medically with one or a combination of drugs. Improved diet management and health care of companion animals have led to increased life expectancy, as a result, cardiac diseases have become pivotal causes of death for both dogs and cats. For such reason, a major objective has become to increase the quality of life by reducing the expansion of cardiac diseases via early detection of diseases and provision of effective treatment.

Introduction

The nucleic acids are the molecular depots that are responsible for continuity of life by transfer of hereditary information from one generation to next. The sequence of nitrogenous bases containing programmed instruction determines the structure of every cell constituent and eventually every protein. In 1869, Friedrich Miescher, Swiss chemist, isolated an unknown phosphate-rich substance from pus cells named as nuclein, which was quite different from other organic substances like carbohydrate, protein, and fat. Altmann in 1899 ascertained that the nuclein also had some acidic characteristics and changed its name to ‘nucleic acid’. Later, nucleic acid was also isolated from salmon sperm nuclei as DNA-protaminecomplex. In 1953, James D.Watson and Francis H.C. Crick constructed the model of three-dimensional double helix structure of the DNA molecule which could successfully explain DNA replication.

Scale insects:Pseudaulacaspis cockerelli, Parasaisettia nigra:Coccidae

Attack Adults and nymphs feed on plant sap and cause wilting and drying of seedlings and young plants.
 
Brown soft scale: Lecanium sp. (=Coccus) hesperidum : Coccidae

9.1. Introduction
The three basic need for human survival are- food, clothing and shelter. The requirement of food comes first. Human are omnivorous. They eat mainly natural foods. Before 5000 years ago, cereals were the main food for all civilization and then the complexity of life began to make appearing with the advent of industrialization, flourishing of industry and commerce. Unpolished grains were utilised at first, but in contemporary civilisation, we have begun to prepare them, which results in the loss of essential nutrients. The intake of meats, processed meals, and sugary goods has grown while the general intake of cereals has decreased. People now lack vital nutrients as a result of the departure from natural diets. People began to realise a few years ago that health is a significant concern in addition to flavour, convenience, and quality value. They have thus begun taking nutritional vitamin supplements to make up for the nutrients lost from processed meals. People are increasingly opting for natural nutraceuticals in capsules and tablets, a trend known as medicalization in nutrition, instead of relying on natural food items. Hippocrates emphasized the connection between food and medicine, stating that healthy foods has a strong therapeutic advantages. The ancient Indian medical system known as Ayurveda places a strong focus on the functioning of diet and nutraceuticals in both health and illness.
9.2. What are Nutraceuticals
Stephen De Felice, the founder and chief executive of the Foundation for Innovation in Medicine (FIM), first used the term “nutraceuticals” in 1989 to refer to the broad category of medicines and nutrition. Nutraceuticals are foods or dietary components that not only offer nourishment and energy but also significantly contribute to regulating and preserving the regular physiological processes that keep people healthy. Nutraceuticals included processed meals such as cereal-grains, soups or stews, genetically engineered foodstuffs, herbal items, nutritional supplements, and extracted nutrients. People are increasingly concerned about their healthy wellbeing these days. However, these include a hectic, sedentary lifestyle, unhealthy eating patterns, body image issues, and being afflicted by a number of illnesses. These are the factors driving the global nutraceutical market’s expansion and generating a lot of interest. Numerous health issues of the century, including cancer, type 2 diabetes, heart disease, inflammation, oxidative stress, constipation, and more, can be fought off with the use of these nutraceuticals. Overall, nutraceuticals bring an entirely novel phase in medical science and assist the food companies to promote more creative research strategies. (Dudeja and Gupta, 2017)).

Several centuries ago, Father of medicine Hippocrates, has said “Let food be your medicine”. Our traditional therapies or alternative medicine effectively integrated this concept and include herbal remedies, nutritional interventions like vitamins/minerals supplementation specialized diets etc, and other techniques such as acupuncture, relaxation regimens, and homeopathic medicines. While, in modern medical science medicines are used to cure diseases which may have some inherited side effects. From the final decade of the last century, a renewed interest was seen in traditional therapies and people are thinking of integrated medicine with a focus on prevention of disease rather than its cure. In this context many terms like “dietary supplements”, “nutraceuticals” and “functional foods” were frequently used to address this issue. The term “nutraceutical” was coined from “nutrition” and “pharmaceutical” in 1989 by Stephen DeFelice, MD, founder and chairman of the Foundation for Innovation in Medicine (FIM). Nutraceutical can be defined as, “a food or part of a food, that provides health benefits, including the prevention and treatment of a disease.” However, the term nutraceutical as commonly used in marketing has no regulatory definition. There is confusion between the closely related terms nutraceuticals, functional food and dietary supplement. Kalra (2003) differentiated between these terms as: Functional food provides the body with the required amount of vitamins, fats, proteins, carbohydrates, etc, needed for its healthy survival. When functional food aids in the prevention and treatment of disease(s) and disorder(s) other than anemia, it is called a nutraceutical.

The requirements for a given nutrient is the minimum quantity of that nutrient that will maintain normal growth and reproduction, when all other nutrients are supplied in adequate quantities and at the same time, prevent the development of symptoms of nutritional deficiency.
 
The accurate way to express the nutrient requirements is in amount per day per animal. Poultry are fed in groups and hence, it is not practicable to express the requirements in amounts per day per animal. So, they are expressed in relation to energy content of the diet as poultry eats within limits to satisfy their energy needs. However, the estimation of energy content of the diet is not possible on routine basis and the calculated values may very much from estimated values. Because of these difficulties, the nutrient requirements of poultry are expressed on unit weight basis. 

Pigs are highly prolific among meat producing livestock and also excellent to all other type of farm animals in the efficiency of converting feed into high quality pork. The Nutrient requirements expressed for the mono-gastric animals like poultry and pigs as percent of the diet or the amount per kg of the diet, since they are generally fed in groups rather than individual feeding practiced in case of cattle and buffaloes (where absolute requirements are given). The latest requirements available are NRC nutrient requirements for swine, 1998 (10th revised edition), BIS, 1986 specifications for pig feeds and ICAR, 1998 for growing and finishing swine. Similarly ARC requirements are also available. NRC requirements are on 90% DM basis and are for breeds like Large White Yorkshire, Landrace, Berkshire etc., while BIS specifications are on 100% DM basis and for indigenous pigs, improved pigs and crossbred pigs.

Goats are valued as economical milk producer and main source for meat for a large section of population. The dry matter intake varies from 3% (Meat goat) to 4-6% (Dairy goat) in different breeds of India and it is higher than that of larger farm animals.

1. Nutrient requirements for maintenance

Adult body weight of goats may vary from 15 to 45 kg in different breeds. There are also does and bucks that weight up to 60kg. Nutrient requirements for maintenance of adult goats are, therefore given for body weights ranging from 15 to 60 kg. On an average for maintenance 76 g DM is required for each kg of metabolic body weight and similarly the requirements for DCP and TDN is 3 and 30 g, respectively.

2. Nutrient requirements for pregnant does

Since adult body weight as indicated already varies from 15 to 60 kg, the requirements for pregnant does are also given for body weights ranging from 15 to 60 kg.

Indian breeds of sheep have much lower adult weight (30-40kg) and growth rate in comparison to many foreign sheep breeds (70kg). Hence the requirements are lesser then foreign breeds.

1. Nutrient requirements for maintenance
On the basis of several balance studies and digestibility trials the requirements for Indian sheep breeds has been developed and calculations are made on the basis of 2.73 g of DCP per kg of metabolic body weight and TDN and ME requirements are 27.3 g and 98 Kcal per kg of metabolic body weight respectively. The requirements for different body weight are given in the table below:

Nutrient requirements of the animals in terms of protein, energy, minerals and vitamins are the basis for scientific feeding. The total requirement of an animal includes two components (i) maintenance (ii) production. While maintenance requirement varies with body size of the animal, the requirement for production depends upon its physiological state and production levels.

Such recommendations are based on the results of a large number of feeding experiments, conducted throughout the world. Feeding standards developed by Nation Research Council (NRC) of USA and Agricultural Research Council (ARC) of UK, for livestock and poultry are considered to be the best general guides for computation of ration. However, limited investigations carried out in this country suggest that adoption of foreign standards always leaves a fair margin of safety so far as the feeding of Indian species are concerned. This owes mainly due to differences in size, breed, nutritional values of feeding stuffs and other practices in the agro-climatic conditions of this tropical country. Some efforts have been made by India scientists in this area.

Duck production has an immense role in improving the socio-economical status of rural farmers. In India, duck farming occupies an important place next to chicken farming. Duck contributes about 7-10% of the total poultry population of the world. Ducks are water fowl, hence, they need a semi-aquatic lifestyle. They need a pond, lake, river, canal or any such type of water bodies for their survival. Thus, they are mainly concentrated in West Bengal, Assam, Andhra Pradesh, Orissa, Kerala, Tripura, Tamil Nadu, Bihar, Jammu & Kashmir and Manipur. Ducks are reared for both eggs and meat purposes. Unfortunately, duck farming is not done in an organized way in our country.

Developments of feeding standards

Feeding standards are statements or quantitative descriptions of amounts of one or more nutrients needed by the body. Since the beginning of nineteenth century, scientists worldwide started formulation of standards for feeing the livestock and poultry and as such with the advancement of science the standards were developed from time to time. For convenience all such standards are grouped under major 
3 headings based on principles of standards. These are comparative type, digestible nutrient system and production value type.

Introduction

Nutrition is the fundamental pillar of life and an essential foundation of human and national development. Rightfrom the earliest stage of human development, the fetal stage, at birth, infancy, childhood, adolescence, adulthood and old age, nutrition forms the basis for survival, performance, mental and physical development & economic and social development.Nutritional deficiencies during pregnancy, fetal and early postnatal life can have a negative impact not only on the subsequent growth and development but can also lead to changes in metabolic regulation and homeostasis.

Introduction

"The new science of nutrigenomics teaches us what specific foods tell your genes. What you eat directly determines the genetic messages your body receives. These messages, in turn, control all the molecules that constitute your metabolism: the molecules that tell your body to burn calories or store them. If you can learn the language of your genes and control the messages and instructions they give your body and your metabolism, you can radically alter how food interacts with your body, lose weight, and optimize your health." (Mark Hyman 2006).

Nutrients have long been considered merely as fuel or cofactors however, it is accepted that diet and feeding strategies play an important role in determining the basic physiological processes that influence the health and wellbeing of animals and ultimately influence the efficiency of production. Traditional research related to animal nutrition mainly deals with either deficiency or excess of the particular nutrient which affects their health and productivity. Subsequently, it was appreciated that nutrients can themselves participate in the regulation of metabolic pathways, for instance through the allosteric regulation of specific enzymes, or by modulating hormone secretion. Shifting of balance between health and disease states involves the complex interplay of genes and the environment, which includes nutrients. The use of systems biology approach in nutrition has attracted both scientists and industrialists during recent decades and a link between diet and health is well established. However, it has often been difficult to easily evaluate the effects of specific nutrients, combinations of nutrients and nutrient interactions because of the lack of straightforward biological indicators that respond to even the simplest changes in nutrition or nutritional status.

4.1. Introduction
The food is the main factor known to affect the health of an individual. By interference in the protein synthesis, function and metabolites and alteration in genome expression, the healthy condition must be altered to diseased condition. Side by side we can say that the dietary component directly or indirectly modulates the molecular fingerprint and understanding of which is dealt with nutrigenomics. So, by modulation of a particular gene for a specific disease by nutrition, we can prevent the specific disease and promote the health of a population. Consuming fruits and vegetables on a regular basis has been linked to better health outcomes and diminution of several chronic or life style linked diseases.
Concept of Nutritional Genomics
After completion of human genome sequencing, the unique term “Nutritional genomics” tends to replace the old term “Nutrient-gene interaction”. It has been stated that so many polymorphism can influence protein structure and function. There are several regulatory sites where nutrients can impact the flow of genetic information. The two components of the nutritional genomics field are nutrigenomics, which studies how dietary components interact with the genome to regulate changes in proteins and other metabolism, and nutrigenetics, which determines how dietary components affect genetic variations. (Farhud et al., 2010). Nutrients are as environmental influences can interact with genetic material. It is commonly known that DNA metabolism and repair require a range of dietary elements that serve as cofactors or substrates in metabolic pathways. However, little is known about how cofactors and/or micronutrient excess or deficiency affect the accuracy of DNA replication and repair (Caroline and Fenech, 2008). The response to a particular nutrient, which is determined by the individual genotype, must also be taken into consideration, even though the nutrients can influence the development of a particular phenotypic.

Mice are omnivores and can be fed with commercial feeds like pellets or fed with mixed feed which is locally available cereal, pulses and grains.

However, nutritionally requirement of mice

Carbohydrates - 45-55%

Protein - 16- 24%

Fibre - 4-5%

Fat - 4-6%

Supplement vitamin and minerals.

Rabbits are produced for meat, wool and research and as pets or for a hobby. Meat production is the most important commercial aspect of rabbitry. The domestic rabbit is primarily herbivorous and will consume most types of grains, oil seed cakes, brans, greens and hay. Diets provided, whether home grown or commercially prepared, consist almost entirely of ingredients from plant sources. Although, the producers / farmers mostly rely on home grown feeds, a good portion of the rabbit feed presently used is commercial pelleted feed. Since the rabbit can utilize a certain amount of forage, it has a place in food production by making use of some non-competitive feeds.

Carbohydrates exhibit protein sparing action, because proteins are mainly required for tissue – building, that is, general wear and tear in the body. If there is any emergency, say animal is deficient in calories of the diet, then it will use adipose and protein tissues. It is said that proteins and fats are burnt (or oxidized) in the flame of carbohydrate. It means that certain intermediary compounds of glucose oxidation through Krebs cycle, are absolutely necessary for oxidation of proteins and fats. Again, in any emergency, if glucose level of the body goes down, fats and proteins are taken over and they get metabolized faster than the body can take care of the intermediate products. However, ketone bodies – acetone, acetoacetate β – hydroxy butyrate appear in urine. In addition to above, monosaccharides are very important (vital)m structural components of many compounds which regulate metabolism. Among these are DNA, RNA for transfer of genetic information of the cell, which contain ribose and deoxy ribose sugars. Glucuronic acid occurs in the liver and this combines with toxic chemicals and bacterial by – products and hence, acts as detoxifying agent. Hyaluronic acid (a disaccharide) forms matrix of connective tissue. Heparin, a mucopolysaccharide, is very important anti – coagulant. Chondroitin sulfates are present in cartilage, bone, skin and tendon. Glycosides are widely distributed throughout the plant kingdom and a number of them have been used as drugs for animals. 
Lipids: these are the most concentrated form of the stored energy in animal kingdom, because they provide 2.25 times per unit more energy than carbohydrate. They provide insulation for vital organs protecting them from mechanical shock and also maintain body temperature. The cell membranes have phospholipids, for example – Erythrocytes. Prostaglandins exhibit hormonal activities. Essential fatty acids – linoleic, linolenic and arachidonic acids show deficiency symptoms in their absence. Lipid also delays hunger, because it requires longer time to pass through stomach than carbohydrate or protein. They also help in lubrication.

A. Answer the following questions

1.    What is the DM consumption in adult guinea pig? Ans. An adult animal consumes @ 7-8% of body weight.

2.    Mention daily average feed consumption of guinea pigs?

Ans: Growing Guinea pigs: 20-30g, Adult maintenance: 30-50g, Pregnant and lactating: 40-60g.

3.    Write down the peculiarities of digestive system of guinea pig.

Ans. The entire stomach of guinea pig is lined with glandular epithelium and gastric mucosa does not have a keratinized portion.

4.    Why guinea pig cannot synthesize vitamin C?