eChapters

A. Answer the following questions

1.    Why deficiency of potassium in livestock is very rare?

Ans: The potassium uptake by plants is about 10 times more than sodium. So, deficiency of potassiumin livestock is veryrare.

2.    HowZn helpsin vitamin Autilization?

Ans: Zn is a component of alcohol dehydrogenase which has a significant role in converting retinol to retinaldehyde or vice versa foreye vision, reproductionandutilization of Vitamin A

3.    What is parakeratosis?

Ans: This is a Zn deficiency disease in pig characterized by hyper keratinisation, thickening and reddening of skin followed by eruption and scab formation giving an appearance of mange.

4.    Why ruminant have additional requirement of vitamin B12? Ans: Theruminants have an additional requirementfor thevitamin B₁₂ because of itsinvolvementin themetabolismof propionic acid.

5.    Why perosis is also called as slipped tendon?

A. Answer the following questions

1.    Why vitamin Ais called anti-infective vitamin?

Ans: Vitamin A is concerned with the synthesis of muco-polysaccharides distributed throughout the body as in respiratory tract,alimentary tract,reproductive tract,genitor-urinarytractetc. In thedeficiencyof vitaminA,theepithelium lining of these tracts becomes keratinized with the result that it becomes prone to infection. That’s why it is also called as anti-infective vitamin.

2.    Why ducks are less effective than chickens in converting tryptophan to niacin?

Ans: Ducks have a 3-4 folds higher level of picolinic acid carboxylase than chicken which enzyme converts the tryptophanto picolinic acid instead of niacin.

3.    Whyvitamin Cis dietaryessential tohumans, monkey, guinea pigs?

Ans: In these species, due to lack of L-gulonolactone oxidase enzyme which is required for synthesis of vitamin C (from 6 carbon sugars like glucose/ galactose in liver or kidney),they can not synthesize their own requirement in their bodies.

4.    What is vitamin?

A. Answer the following questions

1.    What is omega carbon atom?

Ans: The methyl carbon atom at the distal end of the fatty acid chain is called omega carbon atom

2.    Why mammals can not synthesize essential fatty acid?

Ans: Because of their inability to introduce double bond between the 9th carbon atom and the terminal methyl group of the fatty acid chain.

3.    What is acid value?

Ans: It is defined as the number of milligram of KOH required to neutralize the free organic acids in 1 gm of fat.

4.    What is saponification number?

Ans: It is defined as the number of milligrams of KOH required to neutralize the free fatty acid and saponify the esterified fatty acids in 1 gm of fat.

5.    What is iodine number?

A. Answer the following Question

1.    Why availabilityof bacterial protein is low?

Ans: Because, bacterial protein is protected by cell wall (mucopeptide polymer) which are resistant to enzymatic action.

2.    What is isoelectric pH?

Ans: The pH at which an amino acid exists in solution as a zwitter ion is called isoelectric pH that is the pH at which the amino acid is electrically neutral and has no tendency to migrate towards either electrode.

3.    What is ketogenic amino acid?

Ans: Ketogenic amino acid refers to those amino acids which give rise to acetyl CoA and consequently the potential fatty acids producers. e.g. leucine

4.    Why chicks require higher level of arginine?

Ans: Since they do not have urea cycle and feathers are very high inarginine.

5.    Why chicks require high level of glycine?

A. Answer the following question:

1.    What is carbohydrate?

Ans: Literally means hydrates of carbon. Carbohydrates are polyhydroxy (more than one OH group) aldehyde or polyhydroxy ketoneas in monosachharides; their olymers as in oligo and polysaccharides; their reduction products as polyhydric alcohols; oxidation products as aldonic, uronic, sacharic acid; substitution products as amino sugars.

2.    What is sugar?

Ans: The term sugar is generally restricted to those carbohydrates containing less than 10 monosaccharide residues.

3.    What is dextrin?

Ans: It is the intermediate products of the hydrolysis of starch and glycogen.

4.    What is exergonic reaction?

Ans: That portion of energy change during a reaction which is available to do work is termed the free energy change, designated, “G. when “Gis negative the reaction is said to be exergonic.

5.    What is substrate level phosphorylation?

Ans: Whenproductionof ATPfromADPtakesplacedirectlyduring a reaction, the process is known as substrate level phosphorylation.

6.    Why carbohydrate is a metabolically essential?

A. Answer the following questions:

1.    What is proximate analysis?

Ans: Proximate analysisis a systemforapproximatingthenutritive valueof a feedstuff without actually usingit in a feedingtrial.

2.    Why proximate analysis is called Weende analysis?

Ans: In 1865, two scientists namely Wilhelm Henneberg and FriedrichStohmanndeviseda methodcalledWeende analysis/ proximate analysis at Weende Experiment Station, near the University of Goettingen, Germany. Hence, it is called ‘Weende Analysis’.

3.    Write the six proximate principles?

Ans: Water (moisture), ether extract (crude fat), crude fibre, total ash, crude protein and nitrogen free extract (NFE).

4.    Why ether extract is also called as crude fat?

Ans: As it is composed of true fats (glycerides of fattyacids) along with other substances like hydrocarbons, free fatty acids, fat solublevitamins,pigmentslike carotene & chlorophyll,waxes, sterols,lecithin(phospholipids), alkali substances,volatile oils and resins.

5.    What is NFE?

A. Answer the following questions:

1.    What is concentrate ingredients?

Ans: These are feedstuffs having more than 60% TDN, less than 35% cell wall contents and less than 18% crude fibre on dry matter basis.

2.    What is soilage?

Ans: Pasture whether natural or cultivated when cut and fed green to an animal in its own stall is called soilage.

3.    What is husk?

Ans: Dry outer covering of grains and grams e.g. gram husk, rice husk.

4.    What is fodder?

Ans: Any green plant or crop meant for animal feeding for supply of nutrients and bulk.

5.    What is leguminous fodder?

A. Answer the following?

1.    Why does camel mostly eat carbohydrates during desert travel?

Ans: Because increasedrespiration needed to oxidize body fat and protein which result in an increased loss of water by evaporation whereas carbohydrates oxidation results in lower loss of water.

2.    Why birds require less water than mammals?

Ans: Because uric acid is the end product of protein metabolism and is excreted in nearly solid form with minimum loss of water. Higher evaporative loss in birds is partially balanced bytheir reducedurinarywaterexcretionrelative to mammals. Besides, the breakdown of protein to uric acid provides more metabolic water than does its catabolism to urea.

3.    What is TDS?

Ans: TDS or total dissolved solids or salinity is a measure of the usefulness of water for animals or for crop irrigation.

4.    What is metabolic water?

Ans: When prganic compounds such as fat,protein, carbohydrates are oxidized in the animal body, water us produced as end product along with other usable products. Similarly, when proein, fat and carbohydrates are synthesized liberation of water takesplace. The water which is available to the animal body by such biochemical reaction is known as metabolic water.

5.    What is bound water?

A. Answer the following question?

1.    Mention the factors which affect the chemical composition of green forage?

Ans: Soilcompositionandhealth,applicationofmanureandfertilizer, frequency of irrigation, stage of growth, frequency of cutting and variety of strain.

2.    How does the age of plant affect the chemical composition of forage?

Ans: As the plants advance in age, dry matter, crude fibre and ash increase whereas the crude protein and ether extract decreases.

3.    What is food?

Ans: This is the combination of materials that provides nutrients

4. What is feed?

Ans: It refers to the food but most commonly used to animal food.

5. What is diet?

Ans: It is defined as the mixture of feedstuffs used to supply nutrientsto anyindividual.

6.    Whether mineral content of feeds remain fixed?

A. Write the full form of the following:

i.         AAFCO: Association of American Feed Control Officials.

ii.        AAS: Atomic Absorption Spectrophotometry

iii.       HPLC: High Performance/Pressure Liquid Chromatography

iv.       GLC: Gas Liquid Chromatography

v.        AFRC: Agriculture & Food Research Council.

vi.       AICRP: All India Coordinated Research Project.

vii.      AOAC: Association of Official Analytical Chemists

viii.     ARC: Agricultural Research Council (United Kingdom)

ix.      BIS: Bureau of Indian Standard

x.      CIRC: Central Institute for Research on Cattle, Meerut

xi.     CIRG: Central Institute for Research on Goats, Makhdoom.

xii.    CLFMA: Compounded Livestock Feed Manufacturers Association (of India)

xiii.   COP 22: 22nd Conference of Parties.

r DNA Biotechnology
    
Concept of r DNA
    
DNA Preparation from Tissue
    
Reagents
    
Preparation
    
DNA Buffer (Tris-EDTA)
    
RNase A (20 mg/ml)
    
Toe DNA Preparation for Southern Blots
    
Southern Blot
    
PCR
    
Polymerase Chain Reaction
    
The Cycling Reactions
    
Denaturation at 94°C
    
Annealing at 54°C
    
Extension at 72°C
    
DNA Sequencing
    
The Sequencing Reaction
    
Denaturation at 94°C
    
Annealing at 50°C
    
Extension at 60°C
    
Separation of the Molecules
    
Detection on An Automated Sequencer
    
Assembling of The Sequenced Parts of a Gene
    
Freezing of Buffalo Semen (Jindal, 1995)
    
Tissue Culture
    
Preparation of Media
    
Laboratory Procedure for IVF (Kharche et al., 2008)
    
Source of Oocytes
    
Preparation of Estrous Goat Serum
    
Collection of Oocytes
    
Laparoscopic Ovum Pick-up
    
Oocyte Retrieval from Ovaries Collected form Slaughter 
    
House
    
Slicing Technique
    
Follicle Puncture Technique
    
Follicle Aspiration Technique
    
Follicle Isolation Technique
    
Evaluation of Oocytes
    
In vitro Maturation of Oocytes
    
Preparation of Cells for Co-culture
    
Assessment of Nuclear Maturation After in vitro Maturation of Oocytes
    
Analysis of Nuclear Status is Ascribed to One of the Following
    
Sperm Preparation for in vitro Fertilization of Oocytes
    
In vitro Fertilization of Oocytes
    
Assessment of in vitro Fertilization of Oocytes
    
Assessment of Cleavage
    
In vitro embryo culture

Animal Science Biotechnology Research Centres
    
Current Status and Some of the Achievements in Animal Heatlh
    
National Bioresource Development Board
    
Animal Biotechnology
    
Biotechnology Parks and Incubators
    
National Institute of Immunology, New Delhi
    
Bose Institute, Kolkata
    
Jawahar Lal Nehru University, New Delhi
    
National Centre For Cell Science, Pune
    
Centre for Dna Fingerprinting and Diagnostics (Cdfd) Hyderabad
    
National Institute for Plant Genome Research (Nipgr) New Delhi
    
Institute of Bioresources and Sustainable Development,Imphal
    
Institute of Life Sciences, Bhubaneshwar
    
Bharat Immunologicals and Biologicals Corporation Limited
    
International Centre for Genetic Engineering and Biotechnolgoy (Icgeb), New Delhi
    
Animal Biotechnology Research Abroad

Checks and Balances in Biotechnology Applications
    
Biosafety Issues in Animal Biotechnology Application
    
World Organization for Animal Health (OIE)
    
Recombinant DNA Safety Guidelines (1990)
    
Foreign Trade (Development and Regulation) Act, 1992 (2006)(Draft Amendment)
    
Revised Guidelines for Research in Transgenic Plants and Guidelines for Toxicity and Allergenicity Evaluation of Transgenic Seeds, Plants and Plant Parts (1998)
    
Guidelines for Generating Preclinical and Clinical Data for rDNA Vaccines, Diagnostics and other Biologicals (1999)
    
The Food Safety and Standards Act (2006)
    
Transgenic Animals Bioreactors
    
First Patent in Biotechnology
    
Biotechnology - Ethical Concerns
    
Some Priority Areas in the Field of Biotechnology
    
Suggestions for Future Development of Biotechnology Activities in the Field of Animal Health and Production in India
    
Epilogue

Information Sources for Biotechnology
    
Books
    
General Biotechnology Books
    
Animal Biotechnology Books
    
Review Articles
    
Journals
    
Animal Biotechnology Journals
    
Biotechnology Job Listings
    
The Scientist
    
Patents
    
Requirements for Setting up a Biotechnology Laboratory
    
Essential
    
Beneficial
    
Useful
    
PCR
    
Specifications of a Good PCR Macine
    
Gel Documentation System Electrophoresis
    
Laboratory Safety and Hazards

Environmental Biotechnology
    
Global Warming and Animal Health and Production
    
Genome Mapping
    
Human Genome
    
Livestock Genomics
    
Cattle Genome
    
Pig Genome
    
Bioinformatics
    
Major Branches of Bioinformatics Include
    
List of Bioinformatics Software Commonly Used in Animal Genomics Research
    
Sequence Analysis Software
    
Genetics Analysis Software
    
Functional Genomics Software
    
Biofuels
    
Advantages of Bioethanol
    
Ecological
    
Economical
    
Looking to the Future: The Potential of Second-Generation Biofuels
    
Future Role of Biofuels Depends on Profitability and New Technologies

Vaccines
    
Veterinary v/s Human Vaccines
    
Modern Veterinary Vaccines
    
rDNA Technology to Prepare Vaccines
    
Veterinary Viral Vaccines
    
Live Vaccines
    
Killed Vaccines
    
Genetically Engineered Viral Vaccines
    
Veterinary Bacterial Diseases Vaccines
    
Veterinary Parasitic Vaccines
    
Vaccines against cancer
    
Swine Flu
    
Foot and Mouth Disease
    
Adenovirus Vector Vaccine
    
Johne’s Disease
    
Diseases of Poultry
    
Newcastle Disease in Poultry
    
Avian Influenza (Bird Flu)
    
Rabies
    
Disease Diagnostics
    
The Nucleic Acid-hybridization Technique
    
Diagnostics Based on pcr
    
Diagnosis by Restriction Fragment Length Polymorphisms and Related DNA-based Approaches
    
Diagnosis by DNA Probes and DNA Microarray Technology
    
Antigen-Capture Enzyme-Linked Immunosorbent Assay (ELISA) Proteomics
    
Disease Diagnosis Kits Developed
    
Brucelosis
    
Rabies
    
Canine Distemper and Canine Parvo Virus
    
Canine Hepatitis Virus
    
Foot and Mouth Disease
    
New Castle Disease
    
Egg Drop Syndrome Virus-76 (EDS-76)
    
Proteomics for Disease Diagnostics
    
Nanotechnologies in Diagnosis and Vaccine Development
    
Monoclonal Antibodies
    
Immunotherapy
    
Problems
    
Animal Genomics for Disease Resistance
    
Gene Knockout Technology
    
Gene Pharming
    
Gene Therapy
    
Types of Gene Therapy
    
Germ Line Gene Therapy
    
Somatic Gene Therapy

Reproductive Biotechnology
    
Artificial Insemination (AI)
    
History and Future Perspectives of Artificial Insemination
    
Semen Cryopreservation
    
Buffalo
    
Goat
    
Cryoprotection and Anti Oxidants
    
Cryopreservation and Genetic Stability of Livestock Spermatozoa
    
Insemination Technique
    
Cattle and Buffalo
    
Sheep and Goat
    
Conception Rate with Artificial Insemination
    
Multiple Ovulation and Embryo Transfer (MOET)
    
Advantages of Embryo Transfer
    
MOET Includes the Following Steps
    
Superovulatory Treatments
    
Insemination
    
Recovery of Embryos
    
Synchronization of Recipients
    
Cattle
    
Buffaloes
    
Goats
    
Horses
    
In vitro Maturation, fertilization and culture of Oocytes
    
Historical Development
    
In Vitro Maturation of Oocytes
    
In vitro Sperm Capacitation
    
Scope
    
Limitations
    
Embryo Cryopreservation
    
Ultrasonography in Reproductive Management
    
Ultrasound-guided Transvaginal Oocyte Pick-up (OPU)
    
Embryo Cloning or Nuclear Transfer
    
Blastomere Separation
    
Embryo Splitting
    
Nuclear Transfer Cloning (NT)
    
Embryonic Stem Cell Complementations
    
Limitations and Future Prospects of Cloning
    
Sexing of Embryos and Separation of X and Y Chromosomes Bearing Sperms
    
PCR  in Sex Determination
    
Transgenic Animal Production
    
Gene Transfer Through Direct DNA Microinjection
    
Gene Transfer with Retroviral-Vector Assistance
    
Gene Transfer through Production of Germ-line Chimeras
    
Parthenogenetic Embryos: An Emerging Tool in Reproductive Biotechnology for Multiplication of Superior Germ Plasm
    
Applications of Parthenogenesis
    
Livestock Genomics and Marker Assisted Selection (Use of DNA Level Markers)
    
Stem Cells
    
Vaccine for Fertility Control and Increase Prolificacy
    
Auto-immunization to Increase Prolificacy
    
Biotechnology of Conservation of Livestock Breed Biodiversity
    
Gene for Prolificity: Booroola Merino
    
Role of Biotechnology in Enhancing Production
    
Milk
    
Biotechnology of Rumen Manipulation
    
Genetically Modified Ruminal Microorganisms

Biotechnology of Animal Feeding
    
Probiotics
    
Biotechnology Crops as Animal Feed
    
Biotechnology of Animal Products
    
Some New Products made Possible by Biotechnology Include

Biotechnology Products and Technologies
    
Bottlenecks in Fast Progress of Animal Biotechnology
    
Why Biotechnology should be Used in Animal Husbandry and Dairy Production
    
Contribution of Livestock Sector to Food Basket
    
Biotechnology and Environment
    
Biotechnology of Nutrient Partitioning
    
Genetic Programming for Better Animals
    
Biotechnology of Reproduction
    
Biotechnology of Animal Health

A  disaster  (from  Latin  meaning,  “bad  star’)  is  the  impact  of  a  natural  or man-made event that negatively affects life, property, livelihood or industry often  resulting  in  permanent  changes  to  human  societies,  ecosystems  and environment.  The  event  itself  is  not  a  disaster;  it  is  the  impact  that  is  a disaster. Their possibility of occurrence, time, place and severity of the strike can  be  reasonably  and  in  some  cases  accurately  predicted  by  technological and scientific advances. Hence, we can to some extent reduce the impact of damage  though  we  cannot  reduce  the  extent  of  damage  itself.  Despite  the severe impacts suffered – including trauma, starvation, dehydration, infection, disease and shock-animals are often the silent victims of disasters, and there is a real need around for the provision of effective training and management programmes to ensure that their survival and welfare are adequately accounted for. This demands the study of disaster management in methodical and orderly approach.The World Health Organization (WHO) defines a ‘disaster’ as any occurrence that  causes  damage,  destruction,  ecological  disruption,  loss  of  human  life, human suffering, deterioration of health and health services on a scale sufficient to  warrant  an  extraordinary  response  from  outside  the  affected  community or area (WHO, 1999). It is an event, concentrated in time and space, which causes social, economic, cultural and political devastation and which affects both individual people and communities (Kumar, 1998). 

“It  is not uncommon for animals to exhibit behavioral changes before an impending disaster. Wild animals often vacate areas, gather in strange groupings and sometimes will even enter into human habitats they normally avoid,” animal behavior expert Diana L.Guerrero said.Three days before the earthquake, thousands of toads roamed the streets of Mianzhu, a hard-hit city where at least 2,000 people have been reported killed.