eChapters

Prokinetic drugs are used to stimulate gastrointestinal motility, including the abomasum, in animals like cattle, especially in cases of abomasal disorders such as displaced abomasum or atony. Below are commonly used prokinetic drugs and their doses:
1. Metoclopramide
Mechanism: Dopamine antagonist; enhances gastric emptying andincreases motility. Dose
Cattle: 0.1–0.3 mg/kg subcutaneously or intravenously every 8–12,hours.
Horses: 0.04–0.08 mg/kg intravenously as a constant rate infusion
(CRI).
2. Erythromycin (Macrolide antibiotic with prokinetic activity)
Mechanism: Mimics motilin, stimulating smooth muscle contractions. Dose
Cattle: 8.6–10 mg/kg orally every 8–12 hours.
Horses: 0.5–1 mg/kg intravenously every 6–8 hours.
3. Neostigmine
Mechanism: Acetylcholinesterase inhibitor; increases acetylcholine at neuromuscular junctions.

Objectives

• To keep the laboratory animal house neat and clean
• To maintain healthy stock

The disease may be bacterial, viral, fungal or of parasitic origin. All the measures taken to reduce growth and also to control spread of pathogenic organism is a sanitary measure like ventilation, building without cracks crevices.

Important aspect of good sanitation is insect control or pest control. Most effective measure is to just chase them out. To achieve this :

Vaccination of cattle and prevention of diseases causing huge economic losses is very much essential for economic rearing of livestock as prophylactic and strategic vaccination helps in development of immunity against the vaccinated diseases thereby prevents the animals from developing such infections and consequent diseases. Animals should be vaccinated at specific age and at definite time intervals for optimal protection against the diseases.

Introduction

The control of infectious diseases of bacterial etiology in food animals is often by using collective and simultaneous medication of a group, i.e. the so-called preventive uses prophylaxis and metaphylaxis. These uses are vigorously debated because they involve the mass consumption of antibiotics that is suspected to favour the emergence of resistant bacteria, especially in the gut flora, which is critical for the potential transfer of resistance to humans via the food chain. Metaphylaxis corresponds to the administration of antibiotics to animals experiencing any level of bacterial disease before overt disease occurs, with the time of intervention depending on the detection of disease outbreaks in a few animals in the group. Metaphylaxis is launched after (meta) pathogen contamination, with the goal of the bacteriological cure of infected animals, which subsequently warrants the final protection (phylaxis) against infection outbreaks. In this respect, metaphylaxis should be viewed as a curative treatment occurring early in the time scale of the infection, and should be compared to classical curative treatments of sick animals. The pathophysiological status of the animals and the size of the bacterial load at the infection site are specificities of metaphylaxis that could play an important role in antibiotic pharmacodynamics, including the reduction of resistance selection within the pathogenic inoculum. Recent advances have been made in evaluating the antibiotic doses required for clinical and bacteriological cures, depending on the size of bacterial load at the infection site. The observations that total doses were much lower for early treatments when bacterial loads were small, compared to later treatments of sick animals with higher bacterial loads, reinforces the in vivo relevance of the so called ‘inoculum effect’, whatever its underlying mechanisms.

Productivity measures, such as rate of growth or reproduction have traditionally been used as indicators of biological functioning. If this is regarded as closely related to animal welfare, then high productivity should be evidence for a high state of welfare. In some cases, selection for production has pushed animals to the point where basic biological functioning and body maintenance is compromised. Therefore, Production measures are always misused.  Productivity of the group is a different measure than productivity of the individual. Such group overall is performing poorly but that productivity from a few outstanding individuals makes average productivity look high. Similarly specific individuals who are performing very poorly may be overlooked if average group performance is high. Although commercial organized dairy farms are very limited, but welfare issues are always there in both small and large farm. Some of the important issues in the organized farms are discussed below.

Performing animal means any animal which is used at, or for the purpose of any entertainment to which the public are admitted through sale of tickets. Thousands of wild animals are used worldwide to perform demeaning and unnatural tricks to entertain the public (circuses, side-shows, within zoos, and in advertising, film and television). 
There are number of welfare issues related to performing animals which involves animal fighting, shows, (among domestic animals) or entertaining wild animals.

Pig production is concentrated mainly in the North-eastern corner of the country and consists primarily of backyard and informal sector producers. Though pig population is only 2% of all the livestock animals, Pork production in India  represents 7% of the country’s total meat production. This value is highest compared to any other species of meat animals.

1 Broilers

The broilers are also called young meat chickens; generally attend a body weight of 2 kg at 35-38 days of age with a feed conversion of 1.5-1.8. This improvement in growth is equivalent to reduction of market age by one day per year. This fast growth is possible due to intense feeding good quality feed & growth promoters in less space. However, this type of rearing has challenges many welfare issues in broiler farming.

Sheep and goat rearing in India continues to be traditional even today. This sector is primarily in the hands of poor, landless or small and marginal farmers who raise their animals on natural vegetation, stubbles and supplement tree lopping. The productivity of sheep in India is relatively low with an average body weight gain of 20 to 50 g/day. The major causes for the low productivity are inadequate grazing resources, tropical heat and disease incidences and a lack of awareness about improved management practices.

Long used as beasts of burden, animals in India are still regarded today as little more than commodities rather than sentient beings who feel pain, stress, exhaustion and depression. Low literacy rates and the tendency to follow traditional practices and cultural traditions have left India surely lacking in the area of animal welfare. India holds the majority of the world’s working animal population. Animals that are forced to work in India include bullocks, horses, ponies, mules, donkeys, camels, elephants and dogs. Despite a proud heritage and a culture rooted in tolerance and compassion, animal welfare is largely ignored by Indian society, leading to extreme animal suffering. Our villages and to a great extent, the urban population still allow working animals to be mercilessly exploited in fields, on the streets, in brick kilns, on battlefields, on playgrounds and in timber areas – all in the name of agriculture, entertainment, security and any number of other “justified” pursuits.

India is known for its rich wildlife heritage. In fact, the country is considered one of the best among available mega-biodiversity areas in the world. The landscape and agro-climatic conditions of India make it a unique habitat for wild animals. The government of India has made attempts to conserve wildlife through different projects like Corbett tiger, Elephant projects etc. The wild life management in India is at present ‘preservation-oriented’. This is bound to remain so for a long time because of the significantly depleted status of our wild life population. Indian government in pursuit of conserving the heritage of wilderness has made committed efforts with several wildlife and Forest Acts to ensure protection by declaring the areas as protected under the spectrum of national park and wildlife sanctuaries. The eco-tourism and conservation awareness has created environmental consciousness among the people.

• All green leafy materials in nature have Mg, because it is the structural component of chlorophyll. Wherever such green colour is there, carotenes would be associated. The colour of carotenes is red, but this
red colour is masked by green colour of chlorophyll. Wherever above green colour is there, vitamin K would also be invariably associated. Therefore Mg, vitamin A and vitamin K would be available by – default or what!! {α, β, ϒ, δ – carotene (s)}. Hence, grazing animals generally would be receiving these nutrients. [we humans may also eat fresh green leafy vegetables {spinach (palak), bathua, methi, coriander (dhania), cabbage, knol – khol, capsicum (bell pepper, Shimla mirch) to get above nutrients]
• Vitamins help regulate body functions, keeping the body healthy and promoting resistance to dis – eases.
• Supplementation of micro – nutrients (vitamins and minerals) as pre – mix, have + ve effects on maintenance, growth, and milk production.
• Biotin, niacin, choline improves milk production in dairy cattle.
• Multivitamin tablet = A to Z0NS may be given (preferably natural sources would always be suggested).
• Vitamin ‘B’ complex, vitamins C, K are very much synthesized by rumen microbes.
Importance of vitamins and their requirements in animal health and production:
Tables 2 & 3

Crude protein of feeds contain true protein and non – protein nitrogen. True protein is made up of amino acids and for maximum efficiency, feed must have essential acids in correct proportion, correct balance as well as non – essential amino acids should also be in sufficient amounts. About digestion in simple stomached animals, the true protein is degraded to oligopeptides (less than 10 peptides) in the stomach, subsequently to mono peptide amino acids in the small intestine. Thereafter amino acids are assimilated in the small intestine.
But in ruminants, the situation is complex in the sense that feed proteins get digested in the rumen, but even amino acids are also broken down by the microbes, and thereafter, amino and carboxylic groups are released. Secondly, synthesis of new amino acids or proteins takes place for formation of their own microbial body coat. Because of these reasons, the approach for protein evaluation or expression is different in ruminant animal. Further, non-protein portion of the feed cannot be utilized effectively by non – ruminants like swine and poultry. These animals are usually fed with oil cakes, cereals and
cereal by products, which are poor in non-protein compounds, however, young succulent fodders, clover etcetera are rich in such compounds. In the laboratory, crude protein may be estimated by kjeldahl’s method and true protein may be precipitated from non – protein nitrogen fraction by treating with cupric hydroxide or trichloro acetic acid. The precipitate is filtered off and subjected to kjeldahl’s process. For experimental evaluation of non – ruminants: albino or wistar rats, rabbits, guinea pigs or poultry birds are taken. Casein (milk protein) or albumen (egg protein) are fed to these animals for a period of 4
weeks and any of the following methods are undertaken to look for the protein quality based on response of animals.

A. Answer the following Question

1.    Why availabilityof bacterial protein is low?

Ans: Because, bacterial protein is protected by cell wall (mucopeptide polymer) which are resistant to enzymatic action.

2.    What is isoelectric pH?

Ans: The pH at which an amino acid exists in solution as a zwitter ion is called isoelectric pH that is the pH at which the amino acid is electrically neutral and has no tendency to migrate towards either electrode.

3.    What is ketogenic amino acid?

Ans: Ketogenic amino acid refers to those amino acids which give rise to acetyl CoA and consequently the potential fatty acids producers. e.g. leucine

4.    Why chicks require higher level of arginine?

Ans: Since they do not have urea cycle and feathers are very high inarginine.

5.    Why chicks require high level of glycine?

Introduction

Proteins are the most abundant organic compounds constituting major part of the body. Chemically, proteins are nitrogen containing macromolecules and polymers of L α-amino acids, which serve a wide range of structural and biological functions in animal body. For example, connective tissue made up of collagen, metabolicenzymes, neuro transmitters,signal in gmolecules, structural components of cell membranes and cell organelles, etc. all are chemically proteins.

Before goinginto the details of protein metabolism, first, we need to understand that there are majorly two sources of proteins in our body: dietary protein and body protein. Dietary protein is taken in diet, while body protein indicates the protein and amino acids synthesized in animal body to fulfill the requirements for growth and development. The digestion of proteins will not be discussed here as it has been described in detail in chapter 12. Dietary protein is digested in presence of several enzymes such as trypsin, pepsin, chymotrypsin, aminopeptidases and carboxypeptidases and is converted into mono-, di- and oligopeptides in stomach and small intestine; and finally converted into amino acids. These amino acids are transported to the systemthrough intestinal mucosa via active transport, from where they enter into the cells for further metabolism. Therefore, ‘metabolismof proteins’ is more appropriatelylearnt as ‘metabolism of amino acids’.

Introduction

All the amino acids have in common an amino group and a carboxyl group attached to the -carbon atom; however, they differ in having distinctive side chains, usually called R groups, as is shown in the following  generalised structural formula

Introduction

Aquatic ecosystems are critical components of the global environment and provide a variety of resources for human population, including food, water for drinking and irrigation, recreational opportunities, and habitat for economically important fisheries. Fish is the main source of animal protein for 3 billion people worldwide, providing a valuable protein complement to the food platter worldwide. Fish is also an important source of essential vitamins and fatty acids. Furthermore, it provides an important source of cash income for many poor households and holds a great potential as a source of foreign exchange. However, aquatic ecosystems are increasingly being threatened directly and indirectly by climate change. Global warming with consequent climate variability and extreme weather conditions has a great impact on the aquatic ecosystems resulting in increased temperature variation, ocean acidification and freshwater salinization etc. As per report of IPCC 2018, global warming of 1.5 ºC above pre-industrial levels has been declared (IPCC, 2018) and expected to rise further in future. Thus, variations in temperature, salinity, acidity and dissolved oxygen along with the effects of other anthropogenic activities may exert strong in?uences on aquatic ecosystems and the aquatic animls will be facing these climate-related stressors in near future. Such environmental stressors is projected to alter fundamental ecological processes, geographic distribution of aquatic species, reproductive and immunological performance of species etc.

Protozoa are single celled eukaryotes which are either parasitic or free living belonged to family apicomplexa. These protozoan affects the different birds like chickens, turkey, ducks, pigeons, game birds and also various pet birds. The infection caused by protozoan diseases may affect one system or more than one system of the body depending on the causative agents involved. The common protozoan diseases like leukocytozonosis, chochlosomiasis, cryptosporidiosis, coccidiosis, histoplasmosis causes infection in the ducks. The protozoan diseases cause severe economic loss to the poultry industry due to death of birds, expenditure on the treatment and prevention of disease and loss of egg and meat production.

Introduction

The protozoa (meaning proto- first and zoa mean animals) are considered to primitive and are unicellular organism. The protozoa are Eukaryotic organism having nucleus in which the genetic information is stored in chromosomes of nucleus. In poultry, the protozoan disease categorized into two main group

1. Intestinal protozoan parasites include Eimeria spp. (Coccidiosis), Cryptosporidia (Cryptosporidiosis) Histomoniasis (Histomonas meleagridis), Trichomoniasis (Trichomonas spp.) and Spironucleus (Hexamitiasis).

Coccidiosis is important disease of poultry caused by protozoa of the genus Eimeria. The disease occurs by ingestion of sporulated oocyst ehcih upon action of trypsin rupture in gizzard and proventriculus relealeses sporozoites. The sprozoites penetrate mucosa of intestine and two generation of asexual reproduction occurs (Schizogony) and releases merozoites which mature and produces gamonts. Sexyual reproduction occurs (Gametogony) and produses zygot which mature to form oocysts. The oocysts are passed in faeces. The high moisture (exceeding 30%), immunosuppressive diseses and environmental and managemental stress lead to occurrence of disease. The birds affected with coccidiosis show depression, ruffled feathers, blood tinged diarrhoea and pale comb and wattle. Specific part of intestine is affected by each species of Eimeria. Diagnosis of coccidiosis is done by finding oocyst in faeces under microscope.

Proximate analysis: Weende’s system is followed. Weende was a small village in Germany, near the University of Goettingen at that time (1860), when this system was developed by Hanneburg and Stohmann. Although it is neither true nor approximate, but somewhere in the middle, that is, proximate analysis of the various parameters for assessment purpose; it gives information about the quality of feeding stuff and still followed.