eChapters

Pankaj Kumar Singh and Chandramoni

Introduction

The term “probiotic” is derived from Greek and means pro: for and bios: life (for life) in contradiction to antibiotic which means: against life. The term probiotic was first introduced by Lilly and Stillwel (1965) to describe growth-promoting factors produced by microorganisms. Parker (1974) first specified designation “probiotic”. He defined probiotics as microorganisms or substances, which contribute to the balance of the intestinal micro flora. Crawford (1979) defined probiotics as a culture of specific living microorganisms, primarily Lactobacillus spp. that are implanted in the organism and ensure the rapid and effective establishment of a beneficial intestinal population. Fuller (1989) discussed the definition given by Parker (1974) and considered it too broad, as cultures, cells, and metabolites are also included in antibiotic preparations. He redefined “probiotic” as a live microbial feed additive, which beneficially affects the animal by improving its microbial balance. Havenaar et al. (1992) pointed out that the definition of “probiotic” made by Fuller (1989) was restricted to feed supplements, animals, and their intestinal tract. Therefore, they generalized Fuller’s definition of “probiotic” as a mono or mixed culture of living microorganisms, which beneficially affect the host by improving the properties of the indigenous micro-flora. Through 1989, United States Department of Agriculture (USDA) advised manufactures to use the term: “direct-fed microbial” (DFM) instead of “probiotic” (Miles and Bootwalla, 1991). The USFDA defined DFM as a source of live naturally occurring microorganisms, including bacteria, fungi, and yeast. Vanbelle et al. (1990) pointed out that most researchers considered “probiotic” for selected and concentrated viable counts of lactic acid bacteria. Koh et al. (1992) pointed out that as a biological product for newly hatched chicks a bacterial culture producing acetic acid could be used. Such a culture might be supplied to the chicks either through their drinking water or by the feed. For controlling the biological balance in the chicken’s intestinal tract different probiotics may be used. The US National Food Ingredient Association presented, probiotics (direct fed microbial) as a source of live naturally occurring microorganisms and this includes bacteria, fungi and yeast (Miles and Bootwalla, 1991). According to the currently adopted definition by FAO, probiotics are: “live microorganisms which when administered in adequate amounts confer a health benefit on the host” (FAO/WHO, 2001). More precisely, probiotics are live microorganisms of nonpathogenic and nontoxic in nature, which when administered through the digestive route, are favorable to the host’s health (Guillot, et al., 1998; Thomke and Elwinger, 1998,).

Care and treatment of animals is possible by the combined efforts of all the stake holders of animals shared by the owners, consumers and veterinarians. Although, veterinary services are available throughout the country, involving different stakeholders including veterinarian, there is an entrenched lack of empathy and a profound disconnect among stakeholders when it comes to identifying with the pain and discomfort of animals. 
Veterinarians participate in two ways in animal welfare and animal ethics. One is the role of the veterinarian as an individual member of a profession. The other is the collective role of the profession, as expressed by the professional bodies.

The influence of the vagaries of weather on all species on the earth is ubiquitious. It exerts its profound influence on the health, moods and activities of the humans, on all the Agriculture operations right from the seeding stage to the harvesting stage and on the health, behaviour and production of the animal life. Crop growth, is influenced by the variation of the meteorological parameters such as temperature, rainfall and wind etc. It is estimated that in some countries the losses due to adverse weather conditions amount to as much as 30% of the annual agricultural production. This type of losses, many times prove disastrous in the countries whose economy is mainly agriculture based, like that of ours. While the favourable weather conditions increase crop yield, the adverse weather, damages the crop giving rise to very low yield. The weather elements play an important role in governing the crop performance. Similarly weather elements also found to be controlling the Livestock production. It hence becomes necessary for the agriculturists and the veterinarians to have some basic knowledge of Meteorology to get better equipped for problem solving in their respective fields. All the weather phenomenon whether beneficial or disastrous are enacted in the natural theatre which is called the atmosphere. Hence to start with one has to study the characteristics of the atmosphere before proceeding further in meteorology.

The atmosphere is a thin layer of gases surrounding the Earth?s surface, which is retained by the earth?s gravity. The atmosphere protects life on the earth by absorbing ultra violet solar radiation and reduces the temperature extremes between the day and night. The atmosphere has no abrupt cut-off. It slowly becomes thinner and fades away into space. There is no finite boundary between the atmosphere and space. Three quarters of the atmosphere by mass is within 11 km of the planetary surface. In fact half of the atmosphere is contained within 6km from the surface. The Karman line, at 100 km is frequently used as the boundary between the atmosphere and space. The atmosphere contains by volume roughly 78% nitrogen and 21% of oxygen, trace amount of other gases and water vapour. This mixture of gases is known as air. The atmosphere also contains water vapour in smaller quantities. The amount of all these gases except water vapour is more or less constant while the water vapour is highly variable and yet its presence is essential for the development of any weather phenomenon. Based on the thermal characteristics, chemical composition, movement and density, the atmosphere is divided into four distinct layers. They are troposphere, stratosphere, mesosphere & thermosphere. The first 64–80 km above the earth contains 99% of the total mass of the earth?s atmosphere and is generally of a uniform composition, except for a high concentration of ozone, known as the ozone layer, at 12–30 mi (19–50 km). Because of the pull of gravity the density of the atmosphere and the pressure exerted by air molecules are greatest near the earth?s surface. Air pressure decreases quickly with altitude, reaching one half of its value at about 5.5Km. The density of air at sea level is about 1.2 kg/m³. The atmospheric density decreases as altitude increases.

Roots: The main distinguishing characteristic of roots is their high moisture content, which may vary from 75 to 90 percent. Their CF content ranges from 4 to 13 percent. The organic substance of the root is highly digestible and mostly composed of sugars (50-70% DM). Glucose and sucrose is the primary sugar. Although the CP content of roots is often low, the rumen’s protein degradability is extremely high (80-85%). Mangles, swedes, turnips, carrots, sugar beets and fodder beets are among the most popular root crops used to feed farm animals. Although sugar beet is a significant root crop, it is typically not fed to animals as such because it is cultivated largely for its sugar content. Nonetheless, sugar beet pulp and molasses (from both sugar beet and sugarcane) are significant and nutrient-dense animal products that are by-products of the sugar extraction companies. Although pigs are fed roots with higher DM concentrations, like fodder beet, they are not a favourite diet for pigs or poultry due to their bulkiness.
1. Swedes and turnips: Turnips (Brassica campestris) & swedes (Brassica napus) share a lot of chemical similarities, but turnips typically have less DM than swedes. Glucose and fructose are the primary sugars found.
2. Turnip (Brassica rapa): Turnips are root vegetables of cruciferous family. They are usually grown in temperate climates. Humans like a smaller variety, while animals are provided a larger variety. It has 8–10% DM, 12–13% CP and 75–80% TDN. Cultivars with yellow flesh have higher protein content than those with white flesh. Instead of passing through the animal, the volatile material that taints milk is absorbed by the milk from the air. Both turnips and swedes can taint milk if they are offered to dairy cows during or just before milking time.

Hard Red scale: Aonidiella aurantii :Diaspididae

Both adults and young scales suck the sap from the stem and cause spot yellowing and loss of vigour of the plant. Infested shoots bear reddish brown encrustations of the scale and in case of severe damage the shoots dry up.

The process of civilization and materialist life style has forced man to make laws which govern or rule the conduct of the people of society or community. In the earlier days the relation of human and animal was interdependent and mutual. Now a days man has become self-centered and ignorant of his surroundings. This has led to cruelty on the animals in many circumstances. Therefore, in pursuit of prevention of the cruelty to animals following laws, regulations have made by the government.

Principle

Polyethylene glycol (PEG) is a high molecular weight compound which is inert to rumen fermentation. The concentration in the rumen liquor declines with the passage of digesta from rumen to lower G.I. tract and is proportional to time. Protein free filtrate of rumen liquor gives turbidity in the presence of trichloro acetic acid and barium chloride which is a measure of PEG concentration at different post feeding hours.

Introduction

In ruminants,nutritional studies are very complicated unlike non ruminants where it is quite simple. In non-ruminants, analysis of feed taken and faeces voided by the animal gives overall picture of digestion and nutrient availability to the animal. In ruminants, since it has a complex microbial digestive system (rumen) within the animal digestive system, the analysis of the feed taken and faeces voided will not express the whole processes in the GI tract of the animal. To have complete picture, it is necessary to study at the site of microbial digestion (rumen). For such study, samples from the rumen have to be drawn and analyzed for metabolities, microbial enzymes etc.

A. Multiple choices (you have to choose most appropriate answer among 4 alternatives)

1.    Which of the following bacteria in rumen is not cellulolytic

a. Bacteroides succinogenes

b. Ruminococcus flavefaciens

c. Ruminococcus albus

d. Succinivibrio dextrinosolvens

2.    Which of the following bacteria does not interact with anaerobic fungi

a. R. albus

b. R. flavifaciens

c. Butrivibrio fibrisolvens

d. Fibrobacter succinogenes

Ruminitis is an inflammatory condition affecting the rumen wall, primarily caused by disruptions in the rumen’s microbial environment and metabolic processes. It is often linked to excessive consumption of rapidly fermentable carbohydrates, leading to rumen acidosis and subsequent damage to the ruminal epithelium. If untreated, ruminitis can progress to severe systemic complications such as liver abscesses and endotoxemia, significantly impacting animal health, productivity, and profitability in livestock systems. Nutritional management plays a central role in both preventing and treating ruminitis.
Causes
1. High-starch diets: Diets rich in grains and low in fibre promote rapid fermentation, leading to an overproduction of volatile fatty acids (VFAs) m and lactic acid, lowering rumen pH (rumen acidosis).
2. Low-fibre diets: Insufficient fibre intake reduces chewing and saliva production, which are essential for buffering rumen acidity.
3. Inconsistent feeding practices: Irregular feeding schedules or abrupt dietary changes disrupt microbial populations in the rumen.
4. Other factors: Overfeeding of easily fermentable feeds (e.g., silage or molasses), Stress or illness reducing feed intake and altering rumen function.

For animal rearing it is pertinent to know the technique of keeping and preserving the feed required for the livestock. Apart from the rich pasturage, one may have the longevity of their feeds is immense concern for many. Animal feeds are an amalgam of diverse ingredients, including vegetable oil, sunflower oil. There are also animal fats, like – lard, tallow etcetera, which are included in the fodder. These elements give fodder the targeted input to animal husbandry. The prime factor that occurs and becomes a challenge for many livestock rearing, the cost for their feeds, which would provide quality and quantity in their output. Once the feed is rightly chosen for the animal, that one is rearing, the next challenge arises. Here is somehow animal feeds can be preserved safely, thereby increasing the longevity of the feeds - * Stay safe from the Sun; * Metal cans; * Storage bunkers; * Sliding metal roof bunkers;
* Commodity sheds; * Plastic containers or drums; * Re – usable freezer; * Wood bins.
Important dots
• All feed and ingredients have to be stored in a cool place [ideally below 77 0 F or (77 0F –32) X 5/9 = 25 0C), although this is not possible at outside locations under summer situations].
• Feed has to be kept dry to prevent fungal or bacterial growth.
• Prevention of entry for rodents or insects is essential.
• Stable form of vitamins is important to use.
• Anti – oxidants are important for preserving fats, oils in ingredients and feed.
• Expiry dates are important on the containers, for all feed materials for their shelf – life (for ground grains = 1 month after milling)
• For fats and oils: Opened container: 1 month; un – opened or stabilized: 1 - year post mixing.

Introduction

The dairy sector is one of the most important food sectors, which introduced the physical, chemical, microbiological and sensorial criteria duringprocessing steps and in the final dairy products. Several agents/ingredients of non-dairy origin are commonly used to maintain the quality of milk and milk products during processing, transportation and storage. In general, such ingredients are known as food additives and it can be antioxidants, coloring agents, flavoring compounds, preservatives(naturals and synthetic),artificialsweeteners, vitamins, minerals, stabilizers, emulsifiers and curing agents. From the safety point of view, some toxic agents can come into contact of dairy products duringmilking, storage or processing; such as fungal toxins, heavy metals, antibiotics or radionuclide’s, etc., which need to be identified and processed accordingly. Although addition of food additives in food/dairy product is controlled under country specific food laws or Codex Alimentarius Commission (CAC), few dairyproducts such as butter and unflavored fermented milk products are highly restricted for food additives.

For a researcher, laboratory is another home where he/she spends much time every day. As we take care of cleanliness, safety, and design of our home, we must take care on these aspects of laboratory too. It is the responsibility of the laboratory personnel to ensure cleanliness/safety of the laboratory. Since every laboratory chemical and equipment have the potential to cause harms if not used with appropriate precautions. Hence, as a laboratory user, it is our responsibility to follow the basic safety/biosafety rules and regulations set up by the Principal Investigator of the laboratory, Institutional Biosafety Committee, as well as by other regulatory authorities, if any. We must be aware of the general/personal protective measures, cleanliness and laboratory waste disposal prior to joining a research laboratory. In this chapter we will briefly discuss about personal safety, chemical safety, cleanliness, waste disposal, etc.

A. Personal Safety

1. Personal Protective Equipment (PPE)

Personal protective equipment (PPE) is special arrangement to protect the worker from specific hazards because of using a hazardous substance. PPE does not reduce or eliminate the hazard, but protects the wearer from exposure to hazardous material. PPE should not be reused or brought outside the laboratory as these might increase the risk of spread hazardous material or contamination. Used PPE must be kept inside the laboratory in a designated waste storage area adjacent to the work area.

Sales management as the term implies means management of sales. Initially the concept consisted of managing the sales workforce. Now-a-days, it includes managing of all the sales related activities like advertising, sales promotion, physical distribution, pricing and product merchandising.

Blood Sampling

Blood samples shall be taken in the early morning hours before offering any diet to human subjects or ration to livestock. The syringe and needle should be autoclaved, needle should be free from any blockage etc. In the case of ruminants, blood sample is taken directly from jugular vein, while in swine, blood sample is drawn from ear vein, while in human subjects, blood is taken from prominent vein on the inner surface of the yellow usually the (medium basilic). The container (test tube etc.) should be dried and autoclaved. We should separate plasma (from anticoagulent mixed blood) and serum from ordinary blood by centrifugation immediately. We should remember by heart  that blood samples should not be kept in deep freezer, because there are chances of haemolysis as well as an abnormal distribution of certain ions and enzymes between the cells and the serum. We should never force the blood through the needle as such type of practice may result in extensive hemolysis. We should discard first few drops of blood, while taking sample. We should prepare protein free filtrate immediately. Protein-free filtrate may be kept in a much better way than whole blood or plasma.

For any statistical analysis, the required primary data are usually collected by the census or sampling methods. In the census method, the data are collected from all the individuals. E.g., If we want to study the weight of 100 cows, we have to make use of all 100 cows for data collection. That is, we collect data from the entire population or universe. However, in the sampling method, the data are collected from a small group of the population which is termed as the sample. A sample is a portion of the population selected to represent the population. E.g., we collect data only from a sample of cows, say 20 cows drawn from the population.

Sanitation in the Rabbitry is the best disease-prevention system. Daily cleaning of cages or hutches, containers and surrounding is the easiest way to ensure sanitary condition and hence control disease. The most common diseases found in rabbits are cocciodosis. Pasteurella type pneumonia and enteritis. There are generally caused by lack of sanitation in the rabbitry. With strict sanitation practices such as cleaning all cages and water containers everyday, removal of manure, soiled bedding. Contaminated feed and collecting roughage from uncontaminated areas can drastically reduces chances of any disease in the rabbitry.

Scale: Saissetia coffeae: Coccidae

Scale insects

Brownish black scale infests lower surface of leaves along with mid ribs and mid veins.They also cover petioles and tender shoots.  Due to draining of sap, leaves turn yellow and drop, plants become stunted.

The study of animal nutrition is a complex and important field that requires a detailed understanding of the various nutrients that are essential for the proper functioning of an animal’s body. These nutrients, including proteins, carbohydrates, vitamins, minerals, and fats, play a crucial role in metabolism and serve different functions such as providing energy, promoting growth and development, maintaining bodily functions, and supporting immunity. It is imperative to have a thorough knowledge of their quantitative requirements and the sources from which they can be obtained for scientific feeding practices. This knowledge has been gained through extensive research and experience over the years. Therefore, it is crucial for students studying nutrition to understand the methods used to acquire this knowledge and how it continues to evolve through ongoing research. This in-depth understanding will enable them to make informed decisions when it comes to providing optimal nutrition for animals.
Advantages of Scientific Feeding
Scientific feeding offers numerous advantages compared to standard feeding practices.
1. It guarantees that animals receive the correct amount and balance of nutrients, leading to improved health and a decreased risk of metabolic disorders.
2. It enhances feed utilization efficiency, resulting in lower production costs and reduced environmental impact in animal agriculture.
3. It improves the quality and safety of animal products, boosting their market value and consumer acceptance.
Challenges of Scientific Feeding
Despite its benefits, scientific feeding also encounters some challenges.
1. It requires skills and knowledge that small farmers may not have or can’t afford.

Introduction

The original concept of feeding bacterial direct fed microbial (DFM) to livestock was based primarily on potential post–ruminal effects, including improved establishment of beneficial gut microflora (Fuller, 1999). Calves fed Lactobacillus acidophilus have been reported to have reduced incidence of diarrhoea (Beecham et al., 1977) and reduced intestinal coli form count (Bruce et al., 1979). But now it is known that certain bacterial DFM also have beneficial effects in the rumen and one of the important groups of this type of bacteria is propionic acid producing bacteria (Elsden, 1945; Johns, 1951a; Langa, 2010). This group of bacteria includes Propionibacterium spp., Veillonella spp., Megasphaera spp., Selenomonas spp. and Succiniclasticum spp. (Table 1). Propionic acid producing bacteria (PAPB) are secondary bacteria which enhance propionate production by utilizing the end products of primary fibrolytic or starch utilizing bacteria which produce succinic acid and lactic acid (Swenson and Reece, 1996). Inoculation of in vitro fermentation with the lactate utilizing ruminal bacterium Megasphaera elsdenii prevented lactate accumulation and produced propionic acid when a highly fermentable substrate was used (Kung and Hession, 1995). Propionibacteria are also rumen active bacteria which can produce propionic acid by utilizing glucose, lactic acid and succinic acid (Elsden, 1945; Grinstead and Barefoot, 1992; Hugenholtz et al., 2002). Ghorbani et al. (2002) also reported that Propionibacteria convert lactate and glucose to acetate, propionate and CO₂. They also reduce nitrate to nitrite and reduce nitrite load by 40-60% and minimize changes in blood nitrite and met hemoglobin (Rehberger et al., 1993). Succinic acid is an important end–product, but in practice this is converted into propionic acid by other bacteria such as Selenomonas ruminantium (Swenson and Reece, 1996; McDonald et al., 2002; Langa, 2010); such interactions between microorganisms are an important feature of rumen fermentation. Veillonella gazogenes are also active in the rumen and produce propionic acid by fermenting succinic acid (Johns, 1951a). Moreover in low quality forage-based diet Fibrobacter succinogenes are predominant fibrolytic bacteria which digest fibre faster and produce sufficient amounts of succinic acid in the rumen (Cheng et al., 1984; Miron and Ben-Ghedalia., 1992) and synergistic increases in propionate production and fiber digestion was found in co-cultures of Fibrobacter succinogenes and Selenomonas ruminantium (Sawanon et al,2003), and of Ruminococcus flavefaciens and Selenomonas ruminantium (Sawanon et al., 2006). These findings suggest that positive interactions occur between bacteria, mostly via metabolite exchange.