eChapters

Introduction

Gastro-intestinal parasites are considered as the 'silent killers' and remain hidden or undiagnosed by the livestock owners or veterinarians as the infected animals manifest meagre clinical signs. These parasites often have detrimental effects on the growth and development of animals where they can potentiate mortality especially among young, weak or nutritionally compromised animals. They negatively impact animal health in terms of reduced weight gain, anorexia, digestive disturbance, impaired reproductive performance, condemnation of affected organs, mortality in infected animals and ultimately affect animal production, contributing to huge economic losses to the livestock owners. At present, chemotherapy is the most practical and widely practiced approach to curb these infestations. But frequent and indiscriminate use of anthelmintics imposes a threat on the sustainability of current control measures. Thus, there is an urgent need for incorporation of alternate control approaches while designing our worm control programs, so that we can reduce the usage of anthelmintics and prolong their effectiveness. The effectiveness or success of any worm control strategy is evaluated based on integrated parameters like the ultimate goal of increasing production, minimizing risks regarding drug resistance and considering consumer's concerns about health and environment-associated problems. In general, gastro-intestinal nematode control strategies can target either the parasite population inside the host or the free-living stages of parasites in the environment. Here, we are discussing some of the components of sustainable control measures for these parasites in detail.

Sweet potato weevil: Cylas formicarius: Apionidae

It is a specific pest of sweet potato and the most important one. Adult weevils are ant-like, slender bodied having elongated snout-like bluish-brown head with geniculate antenna, bright red thorax and leg tips and bluish-brown elytra. Females make small cavities on the tubers or stems and lay eggs singly. Each female lays 180-200 eggs. Grubs are elongate, legless, pale-yellowish white in colour with smaller brown head. Pupation takes place in larval tunnels in the tuber. Incubation, grub and pupal stages last for 5 to 8, 14 to 17 and 4 to 5 days respectively. Life cycle is completed in 4-5 weeks and adults live for 95-110 days. The grubs bore into stems, tunnel inside and feed on soft tissues. Grubs and adults bore into tubers both in field and storage godowns. Affected tubers develop dark patches, which later start rotting. Feeding holes and adult emergence holes can be seen on vines and tubers.  In severe infestation the collar region of the plants shows unusual thickening due to proliferation and hypertrophy of tissues causing a characteristic terpenoid odour.  Even the slightly damaged tubers are unsuitable for cooking and consumption.  Hence yield loss in practice may come to 100% in many cases.

A. Answer the following questions:

1.    What is flushing?

Ans: It means putting the sow on a more nutritious ration before breedingseasonto improvephysical condition of the female, prominent heat symptoms, increases ovulation rate, shortens period between weaning to successful conception, more uniform litter size and minimizes embryonic death, prompt post weaning estrus.

2.    What is piglet anaemia?

Ans: It is a condition occurs 2-4 weeks after birth when piglets do not have access to soil or pasture and are housed in concrete floor. Piglet anemia is characterized by labored spasmodic breathing(popularlyknownasthumps); hemoglobinlevelfalls to 3-4mg/100ml of blood, poor growth rate, wrinkled skin, rough coat, lack of healthy pink color of visible mucous membrane.

3.    Why pig industry in India has not yet been developed?

Ans: It is mainly due to the fact that pig is not used as meat by majority of meat eaters.

4.    Why it is advisable to offer cereal based ration to the piglets only after two weeks?

India enjoys one of the largest livestock wealth in the world India has continued to experience an increasing growth in its livestock sector. Between 2010-11 and 2019-20, gross valueadded (GVa) from livestock sector grew at an extraordinary rate of 7.6% annually. Among the livestock species, pig finds an important place as it being reared by socio-economically weaker sections of the rural India) Pig as compared to other livestock species has a great potential to contribute to faster economic return, because of certain important inherent traits like high fecundity, better-feed conversion efficiency, early maturity and short generation interval. Pig farming requires small investment on its housing and equipment. It has immense potential to ensure nutritional and economic security for the weaker sections of the society.The pig grows fast and is a prolific breeder, farrowing 10-12 piglets at a time. It is capable of producing two litters per year under good management conditions. The carcase yield is high (65 -80% of live weight). Pigs convert inedible feeds, forages, and certain grain by products into valuable nutrition’s mean. The faeces of pigs are useful in maintaining soil fertility. Pig population According to the 20 th Livestock census of India pigs comprises 1.7 % to the total livestock population (http://dahd.nic.in). The total Pigs in the country are 9.06 million in the current Census, declined by 12.03% over the previous Census. Out of the total pigs 20.98% are either exotic or crossbred

Origin and Domestication of Pigs

Pigs are nonruminant, even-toed ungulates and belong to suborder Suina which includes hippopotamuses, peccaries and pigs. All true pigs are members of the family Suidae which includes five living genera namely Sus, Babirousa (babyrusa), Hylochoerus (giant forest hog), Phacochoerus (warthog), and Potamochoerus (bushpig and red river hog) that can be called as pigs. European breeds are believed to be descendants of the European wild pig Sus scrofa and Asiatic or oriental breeds originated from the Eastern Asiatic banded pig, Sus vittatus a successor of Sus crystatus.

Strengths
• Poultry farming generates early and regular income and has shorter generation interval.
• Poultry and poultry products constitute an important component of human diet. The consumption is also increasing at a rapid rate due to low fat content, easy availability and cost effectiveness.
• Poultry is the least cost alternative food source next to fish only and produces more animal protein from the same amount of feed. Two eggs provide 160 calories of energy constituting more than 20 percent of the daily requirement of Proteins, Vitamin A, D & B12, Riboflavin, Folic acid, Pantothenic acid, Phosphorus and Iodine along with fat.
• According to nutritional Advisory committee of India, at least half an egg should be made available to an average individual which workout to be 180 eggs/annum. Poultry farming require less area with high and quick return than any other animal husbandry and agriculture activities.
• Good infrastructure like road, rail, ports, airports, reliable electricity power and easily approachable to major cities of adjoining states.

A suitable site for housing is very important. It is better to select a site which has good drainage, adequate shade like under trees, and with sufficient surrounding space from strong winds. Basically the shelter must protect rabbits from extreme temperatures and predators like snakes, mongoose, dogs and other animals.

Rabbits can be raised inside the building also or outside in hutches with a protective roof. A hutch is a small building containing cages for housing a few rabbits (6 to 12) a back-yard scale. They are generally made of wood or stone one and chicken wire mesh. The floor may be made of a stronger ranger mesh or soil floor bedded with straw. Hutches can also be constructed with local building materials such as tree and woven split bamboo, etc.

Introduction

Substances found in many plants are generally related to one of the phenols, pyrogallol or catechol. Tannins are solids, usually extracted by hot water insoluble in ether, chloroform, carbon disulphide, benzene; soluble in alcohol ether mixture, alcohol, water and in ethyl acetate, possessing a bitter astringent taste.

Tannic acid is Pentadigalloyl glucose, on hydrolysis yields digallic acid and glucose. Tannins are water soluble glucosides of polydepsides, polymeric ester of gallic acid. It is hydrolysed enzymatically and some efforts have, therefore, been made to preserve tannic, if it is to exert its activity in the duodenum. On the basis of physical or chemical principles which they employ, the methods of tannin analysis  can be grouped as follows.

Tea tortrix/flushworm: Homona coffearia:Tortricidae

The tortrix is  an important pest of tea leaves. The larvae make leaf nests by webbing the leaves using silken threads and feed from inside. A single caterpillar makes several cases. Young larvae prefer tender leaves, while the older larvae are seen in mature leaves. The economic produce being the leaves, and since chemical control is not advised, especially during the picking phase, the pest becomes a management problem.

Techniques to Conduct Digestion Trial Under Stall Conditions

Digestibilty definition. The digestibility of a food is most accurately defined as that  proportion which is not excreted in the faeces and which is , therefore, assumed to be absorbed by the animal. It is commonly expressed in terms of dry matter and as a percentage, the digestibility coefficient. For example, if a cow ate 11 kg of hay containing 10 kg of dry matter and excreted 4 kg dry matter in its faeces, the digestibility of the hay dry matter would be

Introduction

Germfree (GF) animal is one which is isolated from all demonstrable living microorganisms like bacteria, virus, fungus, parasite, algae, yeast. The aim of GF work is isolation (Prevent contamination from air, water, food and environment). It can be attained mechanically and scientifically. GF animal research began in 1895 but in 1897 Nuttall and Thierfelder started to rear GF guinea pigs in their lab. Chicks are classical animal for GF research. Rats and mice are reared through reproduction. Permanent breeding colonies of these have been established.

Synonyms : Axenic animal –meaning free from strange. Gnotobiotic – known life derived from Greek word-Gnoto & biota means known flora & fauna.

Gnotobiote (Gnotobiotic animal ) : Is an animal derived by aseptic caesarian or sterile tatching of eggs that is restored & continuously maintained with Germ free technique under isolator condition in which flora & fauna is clearly defined.

Flora animal: A gnotobiote maintained under isolator condition in international association with one or more known types of microorganisms.

Committee recognized throughout world on laboratory animal called International Committee on Laboratory Animal (ICLA) This committee is responsible to make the rules and regulations and laws which governs production, maintenance and usage of laboratory animal. This committee has defined Specific Pathogen Free (SPF) animal which are free of specified micro organisms and parasites but not necessarily free of the ones which are not specified. Example pasteurella free SPF animal.

Synonyms of SPF Animals

• Disease free animal
• Healthy animal
• Pathogen free animal
• Clean animal
• Caesarian derived animal

The main aim for the production of GFA is for isolation of animal free of all organism.

Introduction

During the last decade, there has been a dramatic increase in use of artificial insemination (AI) by commercial swine producers in many European countries. Till 1990, less than 7% of sows & gilt in the USA were bred by Artificial Insemination. Today, over 70% of the animals are bred by AI. Maybe in a couple of years all swine producers will probably utilize this technology.

In India, it is still in infant stage. Some research work has already been done in scattered ways in various Research Institutes but it is still in the four walls of the laboratory. Some Govt. farms have been doing AI in swine with liquid semen.

Pig is the most important livestock in all Northeastern Hill States and there is no superior indigenous germplasm available in this region. Productivity of local pigs is very low. All through, improvement programmes had undertaken in different state Govt. farms, Central Govt. farms through upgrading with the help of suitable exotic pig breeds like Hampshire, Large Black, and Large White Yorkshire etc. Superior germplasm was distributed to farmers for better productivity. But, improvement till date is not impressive due to lack of superior germless, high feed cost, lack of breeder farmers and lack of incentives to the poor farmers. Maintenance of breeding boar for small farmers is very expensive not only in individual capacity but also on community basis. So, AI is the only tool for quicker, safer, quicker and economic why out for swine improvement like cattle.

The most of the animal population of the world?s lies in regions where frequent seasonal stressors adversely influence the health and productivity. The adaptation of animals to thermal stress is a physiological; biochemical process under endocrine control opens new opportunities to use of endocrine regulation as means of improving thermal tolerance. The animals raised in the semi-arid tropics, usually exhibit low productivity because of shortages of feed and harsh climatic conditions (Maurya et al., 2004). Heat stress can impact animal production and profitability in dairy cattle by lowering feed intake, milk production and reproduction. The animal production is affected by elevated ambient temperature which intern affects the availability of quality feed fodder and water. The indirect effects of climate driven changes in animal performance result mainly from alterations in the nutritional environment of the grazing livestock (Topp and Doyle , 1996). In tropical region most of the time is being spent by animals in the search of feed and fodder. The excess walking stress in the livestock reduces their body condition score and oftenly animal exposed to negative energy balance (Maurya et al., 2010). Under present climate conditions, the lack of ability of animals to dissipate the environmental heat determines that, in many areas in the world, animals suffer heat stress during, at least, part of the year. Heat stress has a variety of detrimental effects on livestock (Fuquay, 1981), with significant effects on milk production and reproduction in dairy cows (Johnson, 1987). The severity of stress and milk production by a cow depends on THI, length of heat stress period, air flow, size of cow, dry matter intake, water availability and coat colour.

At the moment India is world leader in milk production, closely followed by the USA, with a production volume of 84 million tons. More than half of the milk is produced by buffaloes. India has about three times as many ?dairy? animals as the USA, the vast majority (over 80 percent) being kept in herds of 2 to 8 animals. Annual milk yield per dairy animal is about one tenth of that achieved in the USA and about one fifth of the yield of a New Zealand dairy cow. As per India?s National Dairy Development Board (NDDB), the country produced 121 mt of milk in 2010-11 fiscal, which comprised about 17% of global production.

Livestock are homeotherms and has to maintain their body temperature at a fairly constant level through various means of heat gain and heat loss mechanisms. In order to survive in an environment for longer period the energy gain must equal to energy loss. The remarkable interaction has occurred between climate, soil, plants and animals during course of evolution. The maintenance of high level of production in hot regions is difficult as the large amount of heat has to be dissipated besides heat imposed by the environment. Similarly in cold regions, the animal has to produce extra heat to meet the thermoregulatory demand. Considerable variations occur between different species, and between different individual within a species in their capacity to adjust to environmental stress. The farm animals prefer an internal body temperature range from 36-40°C. Normal body temperature of livestock species are:

Cattle: 101-102^oF, Buffalo: 100.5-102°F, Sheep: 102-103°F and Goat: 102.5-103^oF

All the metabolic reactions are under control of various enzymes. The optimum ranges of temperature for activities of these enzymes incidentally fall in the range of normal body temperature. The body keeps its core temperature constant at about 37°C by physiological adjustments controlled by the hypothalamus. A group of neurons, located in anterior hypothalamus, are sensitive to change in skin as well as blood temperature. This hypothalamic centre acts as a “thermostat”. When core body temperature deviated from the thermostat temperature, numerous thermoregulatory mechanisms are initiated to bring back the core body temperature normal. However, in certain instance eg. fever the set point temperature of thermoregulatory control centre is raised. The hypothalamic thermostat works in conjunction with other hypothalamic, autonomic and higher nervous thermoregulatory centers to keep the core temperature constant. Some of these thermoregulatory responses are involuntary, mediated by the autonomic nervous system, some are neurohormonal and others are semi-voluntary or voluntary behavioral responses.

Introduction

Test of pollen viability (extent of pollen viability) is important in pollination experiments to ensure fertility/seed setting. It is also important in monitoring state of pollens during storage in ecological/ taxonomic studies as well as during research on pollen biochemistry, genetics, stigma interactions, and incompatibility studies. Pollen viability refers to the ability of pollen to accomplish post-pollination events to achieve fertilization. Pollen viability test has gained importance particularly in plant breeding and hybrid seed production experiments/ practices. In this chapter, you will be acquainted with the basics of pollen viability test. However, for more professional knowledge on the topic readers are suggested to refer specialized books.

Pollen Viability Test

It is important to know the extent of viability of pollens used in pollination experiment, in finding fertility of a plant of interest, to monitor state of pollen on storage, in ecological/taxonomic studies, as well as in research on biochemistry, genetics, stigma interaction, incompatibility assay, etc. of pollen. Several pollen viability tests have been developed time-to-time. It is recommended to initially use different tests for a given pollen type to find out the most appropriate method that provides reliable result on pollen viability. It is important to note that every procedure requires standardization of protocol according to the sample used. Following different tests are used for assessing pollen viability.

1. In-vitro Pollen Germination Test

It is a simple, speedy, and quantitative test for assessing pollen viability. In this method, a collection/sample of pollen is germinated in vitro and observed under microscope. The percentage of germinating pollens (producing pollen tube) after appropriate treatment and a given period of time is determined. The percentage provides an index of viability of the pollen sample.

Introduction

Seed is a living entity (mature ovule) consisting of a tiny plant in embryonic stage along with the stored food protected by an outer layer known as seed coat. Seed is an important biological input in agriculture, which is economically important for successful crop husbandry. Cotyledons serve as the source of nutrients (proteins, carbohydrates, and oils) that not only nurture the plant at the early developmental (seedling) stages of plant but also the vegetarian (heterotroph) organisms. Being one of the important inputs in agriculture, viability of seed needs to be assessed to know the germination percentage of seeds used to raise a crop. Since viability of seeds may deteriorate on storage depending on the storage conditions. Therefore, often it becomes important to test viability/germinability and vigour of seeds before being used for raising a crop or conducting an experiment. In this chapter, we briefly discuss about the different methods which can be used for testing the viability of seeds.

Seed Viability

Seed viability can be defined as the capability of the seed to germinate and produce a normal seedling within a specified period. In other words, seed viability indicates how many seeds in a lot are alive and could develop into plants under appropriate field conditions. It is very important that most of the stored seeds are capable of germinating when sown in a field. They must possess higher viability at the beginning of storage and maintain it over the storage. A seed with high initial viability survives longer in storage; however, the viability deteriorates slowly at first and then rapidly as the storage prolongs. It is important to know the duration of storage after which a considerable decline in viability occurs under given storage conditions so that to take necessary action to revive the seeds. Excessive deterioration in vianility of seeds may lead to loss of the seed/material/ germplasm.

Nutrition involves various bio-chemical and physiological activities which transformfeed elements into bodyelements. These feed elements are nutrients which are digested, absorbed, utilized to build and renew the components of the animal body. As a result, animal grows and produces-milk, eggs, wool with the help of energy so produced in the body. Afterweaning, most of our farmanimals obtainall of their feedsupply from plants.Barringcarnivores, theplant kingdom is the original and essential source of all animal life, because plants are able to utilize the energy of the sun to build substances which nourishthe animal. Plants make use of carbon dioxide, water and mineral salts to form carbohydrates, fats and proteins which are utilized in the life processes of animal body. Thus plants store and animals dissipate energy.

Plants and their parts

Animal body and plants contain the same substances but they differ in relative amounts. There are also much larger compositional differences among species than do animals. Water is the principal constituent of living plants as well as animal body. With the maturity of seed, water content decreases. The dry matter of plants consists principally of carbohydrates, which serve as both structural and reserve material, while in animals, protein comprises the structure of the soft tissues and fat is the reserve. Thus, animal body contains only a trace of carbohydrate, though occurring as much less than 1 per cent at any given moment, because it is constantly beingformed and broken down in metablolism. Whereas, this is the principal constituent of most of the plant species. Protein is the main constituent of active tissues and thus leaves are much richer in this nutrient than are stems. Grass hays contain less protein than leguminous hays. When theplant matures, there isgradualmovementof protein fromthe vegetative parts to the seed. Thus, the seed contains a higher percentage of protein than the rest of the plant, as in grains and stovers.

Introduction

Canine atopic dermatitis (CAD) is a chronic inflammatory skin disease marked by itching (pruritus), redness (erythema), and recurrent infections. While traditionally considered an immune-mediated condition, recent studies highlight the significant role of skin barrier dysfunction in its development. A key aspect of CAD is the compromised skin barrier, which increases permeability to allergens, irritants, and microorganisms, thereby worsening the disease. The skin barrier is a complex, dynamic structure essential for protecting dogs from environmental challenges. The stratum corneum—the outermost layer of the epidermis—serves as a crucial defense against environmental insults, allergens, and pathogens. In dogs with atopic dermatitis, this barrier often becomes impaired, leading to increased transepidermal water loss (TEWL), greater allergen penetration, and secondary infections. This dysfunction both results from and contributes to the condition, creating a self-perpetuating cycle of barrier disruption, inflammation, and immune dysregulation.

In Vedas the cow has been regarded as the mother of Rudras, the daughter of Vashu and the sister of Aditayas and also the use of cow ghee in religious ceremonies had been mentioned in Rig Veda and there is mention of the cow as “Kam Dhenu” in ancient writings. In Shiva temples are found the references of sculpture of “Nandi Bulls”. In Hindu mythology, the male buffalo thought to be the vehicle of the God of death “Yama” and also regarded as physical form of the demon Mahishasur, a symbol of inertia-”Tamas”.